MBOAT2
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30014008 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of MBOAT2 vs. Ensembl Human
Match: MBOAT2 (membrane bound O-acyltransferase domain containing 2 [Source:HGNC Symbol;Acc:HGNC:25193]) HSP 1 Score: 270.781 bits (691), Expect = 2.130e-81 Identity = 164/445 (36.85%), Postives = 243/445 (54.61%), Query Frame = 2
Query: 98 QPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVL---DNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFD-----PDYFKTDEYLQSGMFY--KYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQ--LTFY 1390
QP+S+AV L +DQ NF+ CQL A F YL++ K S RH++ + G+ L +FCFG H L QS Y +M + ++ + F L +L+V + R+Y +DYG Y+ D +GP+MI TQK++SLA +HDG +K+E L R A+ + P+LLE+ SY F GI+ GP DY+ FIEGR + +N KE+ + + + + P V+ LV GL +F H P + DE+ Q+ + K I L I R KYYFAW++ N +G GF G+ E +D++ N +IE+ TS K +DNWN+ T WL+ Y+R + T+ F+ S+IWHG YPGYYL F++ L T A+RA R R + + L L YDV+T ++ +A++Y +PF L ++ LTFY
Sbjct: 13 QPLSNAVQLPIDQVNFVVCQLFALLAAIWFRTYLHSSKTSSFIRHVVATLLGLYLALFCFGWYALHFLVQSGISYCIMIIIGVENMHNYCFVFALGYLTVCQVTRVYIFDYGQYSADFSGPMMIITQKITSLACEIHDGM-FRKDEELTSS-QRDLAVRRMPSLLEYLSYNCNFMGILAGPLCSYKDYITFIEGRSYHITQSGENGKEETQYERTEPS-----PNTAV-VQKLLVCGLSLLF---HLTICTTLPVEYNIDEHFQATASWPTKIIYLYISLLAARPKYYFAWTLADAINNAAGFGFRGYDENGAARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRVCYERTSFS-PTIQTFILSAIWHGVYPGYYLTFLTGVLMTLAARAMRNNFRHYFIEPSQLKLFYDVITWIVTQVAISYTVVPFVLLSIKPSLTFY 445
BLAST of MBOAT2 vs. Ensembl Human
Match: MBOAT1 (membrane bound O-acyltransferase domain containing 1 [Source:HGNC Symbol;Acc:HGNC:21579]) HSP 1 Score: 231.876 bits (590), Expect = 3.001e-67 Identity = 149/451 (33.04%), Postives = 232/451 (51.44%), Query Frame = 2
Query: 80 SGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGR--QEEVLDNEKEKEYFRK--NKSTILAGWPEIGF-YVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFF 1411
+GS P+S+ +G+ +DQ NF+ CQLVA F F YL S RH + + G+ IFCFG HL + Y +M S + + + +L++ H++R+Y + YG T D +GPLMI TQK+++LAF +HDG ++ E L + HR +K P+ LE+ SY+ F ++ GP DY+ FIEG+ ++L+ +++ F S A ++G V L L F D+F + + Y Y+++ + KYYFAW++ N +G GFSG + +D++ N + +KIE TS K ++NWN+ T WL+ Y R+P Y T+ F+ S++WHG YPGYY F++ L T A+RA R R + +L YD T + LA++Y PF L V+ T ++ M+F
Sbjct: 15 TGSTYLHPLSELLGIPLDQVNFVVCQLVALFAAFWFRIYLRPGTTSSDVRHAVATIFGIYFVIFCFGWYSVHLFVLVLMCYAIMVTASVSNIHRYSFFVAMGYLTICHISRIYIFHYGILTTDFSGPLMIVTQKITTLAFQVHDGLG-RRAEDLSAEQHRLAIKVK-PSFLEYLSYLLNFMSVIAGPCNNFKDYIAFIEGKHIHMKLLEVNWKRKGFHSLPEPSPTGAVIHKLGITLVSLLLFLTLTKTFPVTCLVDDWFVHKASFPARLCYLYVVMQA----SKPKYYFAWTLADAVNNAAGFGFSGVDKNGNFCWDLLSNLNIWKIETATSFKMYLENWNIQTATWLKCVCYQRVPW-YPTVLTFILSALWHGVYPGYYFTFLTGILVTLAARAVRNNYRHYFLSSRALKAVYDAGTWAVTQLAVSYTVAPFVMLAVEPTISLYKSMYF 458
BLAST of MBOAT2 vs. Ensembl Human
Match: LPCAT3 (lysophosphatidylcholine acyltransferase 3 [Source:HGNC Symbol;Acc:HGNC:30244]) HSP 1 Score: 102.449 bits (254), Expect = 3.391e-22 Identity = 99/422 (23.46%), Postives = 185/422 (43.84%), Query Frame = 2
Query: 230 HIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMS-TKSARYFILGFVLLFLSVAHLARMYYDY-GGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRI------PRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIR--PLVHKAS----LNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTP 1453
H+ G+S+ F FG+Q++H L + ++++ M T +A F + +L LA YY G Y + T P + T KL LA + DG K++ +K+A+ P+LLE + +++ +VGP + Y++ ++G E++D K ++I+ + + + + LL+ PH DY T++Y +++ + + I V KY W + I +GLGF+GF E+ K +D N + E I ++N+ T W+ ++ R+ + G +F+ ++WHG + GY +CF L R + I+ P + K + L Y ++ + L + Y F ++ ++F+ H+ + L F+ P
Sbjct: 71 HLFHTFTGLSIAYFNFGNQLYHSLLCIVLQFLILRLMGRTITAVLTTFCFQMAYL----LAGYYYTATGNYDIKWTMPHCVLTLKLIGLAVDYFDG---GKDQNSLSSEQQKYAIRGVPSLLEVAGFSYFYGAFLVGPQFSMNHYMKLVQG---ELIDIPG------KIPNSIIPALKRLSLGLFYLVGYTLLS----PHITEDYLLTEDYDNHPFWFRCMYMLIWGKFVLYKYVTCWLVTEGVCILTGLGFNGFEEKGKAKWDACANMKVWLFETNPRFTGTIASFNINTNAWVARYIFKRLKFLGNKELSQGLSLLFL--ALWHGLHSGYLVCFQMEFLIVIVERQAARLIQESPTLSKLAAITVLQPFYYLVQQTIHWLFMGYSMTAFCLFTWDKWLKVYKSIYFLGHIFFLSLLFILP 470
BLAST of MBOAT2 vs. Ensembl Human
Match: MBOAT2 (membrane bound O-acyltransferase domain containing 2 [Source:HGNC Symbol;Acc:HGNC:25193]) HSP 1 Score: 88.9669 bits (219), Expect = 7.611e-20 Identity = 62/189 (32.80%), Postives = 94/189 (49.74%), Query Frame = 2
Query: 98 QPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYL 661
QP+S+AV L +DQ +R++ +SP+ VC L H L QS Y +M + ++ + F L +L+V + R+Y +DYG Y+ D +GP+MI TQK++SLA +HDG +K+E L R A+ + P+LLE+ SY F GI+ GP DY+
Sbjct: 13 QPLSNAVQLPIDQA----------------ARHMIPGCLSPS-------VCLTYLAESTGEKYALHFLVQSGISYCIMIIIGVENMHNYCFVFALGYLTVCQVTRVYIFDYGQYSADFSGPMMIITQKITSLACEIHDGM-FRKDEELTSS-QRDLAVRRMPSLLEYLSYNCNFMGILAGPLCSYKDYI 176
BLAST of MBOAT2 vs. Ensembl Human
Match: LPCAT3 (lysophosphatidylcholine acyltransferase 3 [Source:HGNC Symbol;Acc:HGNC:30244]) HSP 1 Score: 86.2705 bits (212), Expect = 1.972e-17 Identity = 74/295 (25.08%), Postives = 134/295 (45.42%), Query Frame = 2
Query: 230 HIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMS-TKSARYFILGFVLLFLSVAHLARMYYDY-GGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWL 1108
H+ G+S+ F FG+Q++H L + ++++ M T +A F + +L LA YY G Y + T P + T KL LA + DG K++ +K+A+ P+LLE + +++ +VGP + Y++ ++G E++D K ++I+ + + + + LL+ PH DY T++Y +++ + + I V KY W + I +GLGF+GF E+ K +D N + E I ++N+ T W+
Sbjct: 71 HLFHTFTGLSIAYFNFGNQLYHSLLCIVLQFLILRLMGRTITAVLTTFCFQMAYL----LAGYYYTATGNYDIKWTMPHCVLTLKLIGLAVDYFDG---GKDQNSLSSEQQKYAIRGVPSLLEVAGFSYFYGAFLVGPQFSMNHYMKLVQG---ELIDIPG------KIPNSIIPALKRLSLGLFYLVGYTLLS----PHITEDYLLTEDYDNHPFWFRCMYMLIWGKFVLYKYVTCWLVTEGVCILTGLGFNGFEEKGKAKWDACANMKVWLFETNPRFTGTIASFNINTNAWV 345
BLAST of MBOAT2 vs. Ensembl Celegans
Match: mboa-3 (Lysophospholipid acyltransferase; Membrane Bound O-Acyl transferase, MBOAT [Source:UniProtKB/TrEMBL;Acc:G5EC53]) HSP 1 Score: 216.853 bits (551), Expect = 2.784e-62 Identity = 127/465 (27.31%), Postives = 229/465 (49.25%), Query Frame = 2
Query: 65 MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIR-PLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPK 1456
+ FY GSKI +P+++ +G+ D+ NF+ G +++ + + +++ R ++ V G+ + FCFG I HLL ++ Y +M +L F + +L H R + Y++D+TGP+M+ +K + +AFNL DG+ K++ + ++ +L + P+LLEF S+MF F ++ GP DY++F+ +EK + K G FY L F + + + + + T Y F + I F +R YYFAW SG GFSG+ +E + + N +++E+ S K +D WN+ T +WLR+ Y+R P++ T+A + S++WHG GYY+ F + LFT A++ R+ +R + + YD+ + ++ +AL Y F + + + + +++FI H+ + + F PK
Sbjct: 4 IHTFYDGSKILEPLAETIGIEADRVNFVLSLFACFGISYVYRKTCSLKQVNRQVRTVVPAVVGIGIVFFCFGRAIKHLLANALGSYAIMYFAPPTQVHKIVLLFSMGYLFFIHCYR-WAILESYSLDVTGPIMVAVEKATMMAFNLKDGK--AKDQSKLTEEQKRESLKEIPSLLEFMSFMFNFQTVLTGPANNYYDYIKFL---------DEKHLVADKHGKLPSPTGAAMKKFY--QSLFFLAIVVTLGGKYKVEDVGTQPYFALPFFQWFFWWFITIFFIRCAYYFAWVFADAICNMSGFGFSGYDKEGNAEWKLCTNVLPYQVEMAQSLKETLDGWNIQTGFWLRKVGYERAPKSIRTVATYTLSAVWHGVSIGYYMAFFTCGLFTVAAQTFRRSMRWRFLDDHNKKFAYDIFSFIISKIALAYATYSFVTMHMYPAYDVLKRVYFIPHIVAFAVIFALPK 454
BLAST of MBOAT2 vs. Ensembl Celegans
Match: mboa-3 (Lysophospholipid acyltransferase; Membrane Bound O-Acyl transferase, MBOAT [Source:UniProtKB/TrEMBL;Acc:G5EC53]) HSP 1 Score: 216.853 bits (551), Expect = 2.784e-62 Identity = 127/465 (27.31%), Postives = 229/465 (49.25%), Query Frame = 2
Query: 65 MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIR-PLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPK 1456
+ FY GSKI +P+++ +G+ D+ NF+ G +++ + + +++ R ++ V G+ + FCFG I HLL ++ Y +M +L F + +L H R + Y++D+TGP+M+ +K + +AFNL DG+ K++ + ++ +L + P+LLEF S+MF F ++ GP DY++F+ +EK + K G FY L F + + + + + T Y F + I F +R YYFAW SG GFSG+ +E + + N +++E+ S K +D WN+ T +WLR+ Y+R P++ T+A + S++WHG GYY+ F + LFT A++ R+ +R + + YD+ + ++ +AL Y F + + + + +++FI H+ + + F PK
Sbjct: 4 IHTFYDGSKILEPLAETIGIEADRVNFVLSLFACFGISYVYRKTCSLKQVNRQVRTVVPAVVGIGIVFFCFGRAIKHLLANALGSYAIMYFAPPTQVHKIVLLFSMGYLFFIHCYR-WAILESYSLDVTGPIMVAVEKATMMAFNLKDGK--AKDQSKLTEEQKRESLKEIPSLLEFMSFMFNFQTVLTGPANNYYDYIKFL---------DEKHLVADKHGKLPSPTGAAMKKFY--QSLFFLAIVVTLGGKYKVEDVGTQPYFALPFFQWFFWWFITIFFIRCAYYFAWVFADAICNMSGFGFSGYDKEGNAEWKLCTNVLPYQVEMAQSLKETLDGWNIQTGFWLRKVGYERAPKSIRTVATYTLSAVWHGVSIGYYMAFFTCGLFTVAAQTFRRSMRWRFLDDHNKKFAYDIFSFIISKIALAYATYSFVTMHMYPAYDVLKRVYFIPHIVAFAVIFALPK 454
BLAST of MBOAT2 vs. Ensembl Celegans
Match: mboa-4 (Membrane Bound O-Acyl transferase, MBOAT [Source:UniProtKB/TrEMBL;Acc:H9G2W5]) HSP 1 Score: 202.216 bits (513), Expect = 1.132e-56 Identity = 142/491 (28.92%), Postives = 242/491 (49.29%), Query Frame = 2
Query: 74 FYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIG-SMSNIFSGLGFSGFSEER-KPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLN-LPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLAPLFSSASGDHSPRVADKISGDDISV 1537
FY + +PVS A+ + +DQ NF+ L++ G G+ F R D S T R + G+ T FC+G+ I HL Y++M ++ + + F + +L + H R Y Y +D+TG +M+ K++ LA + DG + ++ ++ A+ + P+LL+F SYMF F ++VGP + + F+E + V NEK + + + + I F V ++ L + + DP +EY + +++ S + R YYFAW+I S+ NI SG G+ G ++E +P + N +E G + K ++DNWN+ T+ WLR VY+R+ Y TLAV+V + WHG GYY F++ ALFT ++ R+ +R S++ L YD+ L+ A+ Y PF + + +++ K+ I H ++ +Y P++ SP+ KI+G + S
Sbjct: 15 FYDPVTLLRPVSRAISMNLDQLNFVTVLLLSFGLGYWF-RVQFCDA-SRTTRAAVTTSVGLIFTYFCYGNAIAHLFINGFGSYLLMVSVPPQHVHKSVFAFAMGYLVLIHSYRWMYQ-KTYCLDVTGSMMVAVGKITLLASAITDG--MGRDPKALNSGQKRDAVKEVPSLLDFASYMFNFQTVIVGPMNHYSTWSAFLELKH--VPKNEKTGKPYDPTSTAMKKFEAAIAFSVVYTILGSYLPMSLTN--DPS---INEY---NLLIWWLITVAASTVHRLPYYFAWTISDSICNI-SGFGYDGLADETLEPKWSRTTNVKPLLVEFGQNYKEMVDNWNIWTVAWLRRVVYERVEGPYRTLAVYVTGAAWHGLAVGYYFSFLTSALFTLSAATFRRCMRHRFLGNSMHKLMYDIFGMLVSKFAIGYIHWPFYVMHFWPSLFMYRKLLMIPHFIALFIYLYLPQI----------FSPQPKSKITGPNASA 479
BLAST of MBOAT2 vs. Ensembl Celegans
Match: mboa-4 (Membrane Bound O-Acyl transferase, MBOAT [Source:UniProtKB/TrEMBL;Acc:H9G2W5]) HSP 1 Score: 177.178 bits (448), Expect = 9.587e-48 Identity = 135/491 (27.49%), Postives = 230/491 (46.84%), Query Frame = 2
Query: 74 FYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIG-SMSNIFSGLGFSGFSEER-KPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLN-LPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLAPLFSSASGDHSPRVADKISGDDISV 1537
FY + +PVS A+ + +DQ NF+ L++ G G+ F R D S T R + G+ T FC+G+ I HL Y++M ++ + + F + +L + H R Y Y +D+TG +M+ K++ LA + DG +P + +K +K ++VGP + + F+E + V NEK + + + + I F V ++ L + + DP +EY + +++ S + R YYFAW+I S+ NI SG G+ G ++E +P + N +E G + K ++DNWN+ T+ WLR VY+R+ Y TLAV+V + WHG GYY F++ ALFT ++ R+ +R S++ L YD+ L+ A+ Y PF + + +++ K+ I H ++ +Y P++ SP+ KI+G + S
Sbjct: 15 FYDPVTLLRPVSRAISMNLDQLNFVTVLLLSFGLGYWF-RVQFCDA-SRTTRAAVTTSVGLIFTYFCYGNAIAHLFINGFGSYLLMVSVPPQHVHKSVFAFAMGYLVLIHSYRWMYQ-KTYCLDVTGSMMVAVGKITLLASAITDGM---GRDPKALNSGQKRDAVK--------------ETVIVGPMNHYSTWSAFLELKH--VPKNEKTGKPYDPTSTAMKKFEAAIAFSVVYTILGSYLPMSLTN--DPS---INEY---NLLIWWLITVAASTVHRLPYYFAWTISDSICNI-SGFGYDGLADETLEPKWSRTTNVKPLLVEFGQNYKEMVDNWNIWTVAWLRRVVYERVEGPYRTLAVYVTGAAWHGLAVGYYFSFLTSALFTLSAATFRRCMRHRFLGNSMHKLMYDIFGMLVSKFAIGYIHWPFYVMHFWPSLFMYRKLLMIPHFIALFIYLYLPQI----------FSPQPKSKITGPNASA 464
BLAST of MBOAT2 vs. Ensembl Celegans
Match: mboa-6 (Lysophospholipid acyltransferase 5 [Source:UniProtKB/Swiss-Prot;Acc:O01925]) HSP 1 Score: 115.161 bits (287), Expect = 6.562e-27 Identity = 94/335 (28.06%), Postives = 158/335 (47.16%), Query Frame = 2
Query: 227 RHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDIT--GPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCS----SIWHGYYPGYYLCF 1213
+H+ ++ G+SL +F GS + H + + + N M+ A + V L HL Y+ + T DIT P I T + L +++DG +K E L+ D + A+ P LLE ++ +F G +VGP L+ + F+ G + LD++ + KS L P IG ++ C + +L + Q YF +D Y F+++ +T+ L KY W I ++I SGLG +G E +D V++ K E G S+++++N GT + + ++ R+ LA V + +IWHGY+ GY+L F
Sbjct: 46 QHLFFVIVGLSLWMFNCGSSVIHPILSIFGAFFITNFMAGTDASIYAAHIVFL----GHLLIGYWFHETDTYDITWTTPFCIMTLRFIGLVMDVYDGA--QKPEHLKPD-QKLTAISDKPGLLEIAAFGLFFQGTLVGPQFTLSKFRSFVNG---DWLDSDGQPP-----KSAFL---PSIGRFLAGC-TYMVLHQWGQFWIPDQYFNSDAYNNLSFFWRWSWVTLWFRLTMYKYCAMWLITEGASILSGLGHNGKDAEGNDRWDGVRDLHIIKWETGHDYNSVVESFNCGTNTFAKNHIHRRLRWVNNKLASHVITLSYLAIWHGYHLGYFLLF 361
BLAST of MBOAT2 vs. Ensembl Fly
Match: oys (gene:FBgn0033476 transcript:FBtr0339416) HSP 1 Score: 298.516 bits (763), Expect = 4.981e-90 Identity = 167/483 (34.58%), Postives = 260/483 (53.83%), Query Frame = 2
Query: 62 IMSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEG----------------RQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQ-SGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLN-LPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPK 1456
I ++ Y+GS+ F ++D VGL+VD NF+ CQ+ A +F L+ K+S RH L G++ FCFG Q H+ G YI++ + + +L + +L HL R YDYG Y +DITGPLMI TQK++SLAF++HDG ++ +E L + + HA+ K P+ LE+FSY+++F I+ GP VF DY+ F+EG ++E VL+ K RK +G V + + + I+ P D K D+++ + M YKY + + +R KYY AW + SGLGF+G+ ++ +D++ N + E T+ + I+NWN GT WLR VY+R+P+ YGTL F S++WHG+YPGYYL F + A+ A+R GR+ R + + YD+LT L+ + L Y PF LE + ++ + + +H+ S++ F+ PK
Sbjct: 8 IENDCYNGSRTFTWLADMVGLSVDLVNFLICQISALFLASLFRSMLHPSKVSSKLRHTFALSIGLAFGYFCFGQQAIHIAGLPAICYIVIRTQDPRIVQRAVLLVAMSYLLCVHLMRQLYDYGSYALDITGPLMIITQKVTSLAFSIHDG-FVRGDEELTK-AQQYHAIRKMPSALEYFSYVWHFQSILAGPLVFYKDYIEFVEGYNLLSTPPGNGNLDSSKREVVLEPSPTKAVIRK----------VVGSLVCAFIFMKFVKIY--PVKD---MKEDDFMNNTSMVYKYWYAMMATTCIRFKYYHAWLLADAICNNSGLGFTGYDKDGNSKWDLISNINVLSFEFSTNMRDAINNWNCGTNRWLRTLVYERVPQQYGTLLTFALSAVWHGFYPGYYLTFATGAVVVTAARTGRRLFRHRFQSTQVTRMFYDILTCLITRVVLGYATFPFVLLEFMGSIKLYLRFYLCLHIISLVTIFILPK 473
BLAST of MBOAT2 vs. Ensembl Fly
Match: oys (gene:FBgn0033476 transcript:FBtr0088434) HSP 1 Score: 298.516 bits (763), Expect = 4.981e-90 Identity = 167/483 (34.58%), Postives = 260/483 (53.83%), Query Frame = 2
Query: 62 IMSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEG----------------RQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQ-SGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLN-LPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPK 1456
I ++ Y+GS+ F ++D VGL+VD NF+ CQ+ A +F L+ K+S RH L G++ FCFG Q H+ G YI++ + + +L + +L HL R YDYG Y +DITGPLMI TQK++SLAF++HDG ++ +E L + + HA+ K P+ LE+FSY+++F I+ GP VF DY+ F+EG ++E VL+ K RK +G V + + + I+ P D K D+++ + M YKY + + +R KYY AW + SGLGF+G+ ++ +D++ N + E T+ + I+NWN GT WLR VY+R+P+ YGTL F S++WHG+YPGYYL F + A+ A+R GR+ R + + YD+LT L+ + L Y PF LE + ++ + + +H+ S++ F+ PK
Sbjct: 8 IENDCYNGSRTFTWLADMVGLSVDLVNFLICQISALFLASLFRSMLHPSKVSSKLRHTFALSIGLAFGYFCFGQQAIHIAGLPAICYIVIRTQDPRIVQRAVLLVAMSYLLCVHLMRQLYDYGSYALDITGPLMIITQKVTSLAFSIHDG-FVRGDEELTK-AQQYHAIRKMPSALEYFSYVWHFQSILAGPLVFYKDYIEFVEGYNLLSTPPGNGNLDSSKREVVLEPSPTKAVIRK----------VVGSLVCAFIFMKFVKIY--PVKD---MKEDDFMNNTSMVYKYWYAMMATTCIRFKYYHAWLLADAICNNSGLGFTGYDKDGNSKWDLISNINVLSFEFSTNMRDAINNWNCGTNRWLRTLVYERVPQQYGTLLTFALSAVWHGFYPGYYLTFATGAVVVTAARTGRRLFRHRFQSTQVTRMFYDILTCLITRVVLGYATFPFVLLEFMGSIKLYLRFYLCLHIISLVTIFILPK 473
BLAST of MBOAT2 vs. Ensembl Fly
Match: oys (gene:FBgn0033476 transcript:FBtr0345630) HSP 1 Score: 298.516 bits (763), Expect = 4.981e-90 Identity = 167/483 (34.58%), Postives = 260/483 (53.83%), Query Frame = 2
Query: 62 IMSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEG----------------RQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQ-SGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLN-LPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPK 1456
I ++ Y+GS+ F ++D VGL+VD NF+ CQ+ A +F L+ K+S RH L G++ FCFG Q H+ G YI++ + + +L + +L HL R YDYG Y +DITGPLMI TQK++SLAF++HDG ++ +E L + + HA+ K P+ LE+FSY+++F I+ GP VF DY+ F+EG ++E VL+ K RK +G V + + + I+ P D K D+++ + M YKY + + +R KYY AW + SGLGF+G+ ++ +D++ N + E T+ + I+NWN GT WLR VY+R+P+ YGTL F S++WHG+YPGYYL F + A+ A+R GR+ R + + YD+LT L+ + L Y PF LE + ++ + + +H+ S++ F+ PK
Sbjct: 8 IENDCYNGSRTFTWLADMVGLSVDLVNFLICQISALFLASLFRSMLHPSKVSSKLRHTFALSIGLAFGYFCFGQQAIHIAGLPAICYIVIRTQDPRIVQRAVLLVAMSYLLCVHLMRQLYDYGSYALDITGPLMIITQKVTSLAFSIHDG-FVRGDEELTK-AQQYHAIRKMPSALEYFSYVWHFQSILAGPLVFYKDYIEFVEGYNLLSTPPGNGNLDSSKREVVLEPSPTKAVIRK----------VVGSLVCAFIFMKFVKIY--PVKD---MKEDDFMNNTSMVYKYWYAMMATTCIRFKYYHAWLLADAICNNSGLGFTGYDKDGNSKWDLISNINVLSFEFSTNMRDAINNWNCGTNRWLRTLVYERVPQQYGTLLTFALSAVWHGFYPGYYLTFATGAVVVTAARTGRRLFRHRFQSTQVTRMFYDILTCLITRVVLGYATFPFVLLEFMGSIKLYLRFYLCLHIISLVTIFILPK 473
BLAST of MBOAT2 vs. Ensembl Fly
Match: oys (gene:FBgn0033476 transcript:FBtr0345629) HSP 1 Score: 279.256 bits (713), Expect = 4.787e-83 Identity = 157/460 (34.13%), Postives = 243/460 (52.83%), Query Frame = 2
Query: 131 DQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEG----------------RQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQ-SGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLN-LPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPK 1456
D NF+ CQ+ A +F L+ K+S RH L G++ FCFG Q H+ G YI++ + + +L + +L HL R YDYG Y +DITGPLMI TQK++SLAF++HDG ++ +E L + + HA+ K P+ LE+FSY+++F I+ GP VF DY+ F+EG ++E VL+ K RK +G V + + + I+ P D K D+++ + M YKY + + +R KYY AW + SGLGF+G+ ++ +D++ N + E T+ + I+NWN GT WLR VY+R+P+ YGTL F S++WHG+YPGYYL F + A+ A+R GR+ R + + YD+LT L+ + L Y PF LE + ++ + + +H+ S++ F+ PK
Sbjct: 11 DLVNFLICQISALFLASLFRSMLHPSKVSSKLRHTFALSIGLAFGYFCFGQQAIHIAGLPAICYIVIRTQDPRIVQRAVLLVAMSYLLCVHLMRQLYDYGSYALDITGPLMIITQKVTSLAFSIHDG-FVRGDEELTK-AQQYHAIRKMPSALEYFSYVWHFQSILAGPLVFYKDYIEFVEGYNLLSTPPGNGNLDSSKREVVLEPSPTKAVIRK----------VVGSLVCAFIFMKFVKIY--PVKD---MKEDDFMNNTSMVYKYWYAMMATTCIRFKYYHAWLLADAICNNSGLGFTGYDKDGNSKWDLISNINVLSFEFSTNMRDAINNWNCGTNRWLRTLVYERVPQQYGTLLTFALSAVWHGFYPGYYLTFATGAVVVTAARTGRRLFRHRFQSTQVTRMFYDILTCLITRVVLGYATFPFVLLEFMGSIKLYLRFYLCLHIISLVTIFILPK 453
BLAST of MBOAT2 vs. Ensembl Fly
Match: nes (gene:FBgn0026630 transcript:FBtr0075019) HSP 1 Score: 101.679 bits (252), Expect = 2.617e-22 Identity = 93/383 (24.28%), Postives = 167/383 (43.60%), Query Frame = 2
Query: 104 VSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLN--TDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIG---FYVKSCLVFGLLTIFVQPHFDPD-YFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIP------RAYGTLAVFVCSSIWHGYYPGYYLCFM 1216
++ VG+ V+ + L ++ ++++ DK T H+ CG L F +G +H L ++ Y ++ + K+ + + FV +S L Y Y + T P I ++ F++ DG +K+ L +D ++ AL K P+LLE ++ ++ G +VGP Y F++G FR+++ + AG G FY+ C V GL + PD YF T E+ Q + +L + KY W + + I GL + G + +P + N +E G + + + ++NV T W+ + +Y R+ +YG F+ ++WHGY+ GYY+ F+
Sbjct: 17 IASGVGVPVEALRLLLTILAGYPVAALYQKFISVIADK---TVHHMFFAGCGAGLCYFNYGLDTYHSLIAILTTYFLVLLLRKKTQIFLAINFVF-HMSYLLLGYFYTSSNDYDILWTMPHCILVLRMIGYGFDITDG--LKEESELSKD-QKETALKKPPSLLELLAFSYFPSGFLVGPQFPFRRYKAFVDGE-------------FRQHEGNVEAGVRRFGAGAFYLIVCQV-GL-------RYLPDSYFLTPEFAQVSFVKRIYLLGFWAKFSLYKYISCWLLTEGALICIGLTYKGEDKNGQPDWSGCSNVKLKLLETGNTMEHYVQSFNVNTNQWVGQYIYKRLKFLNNRTISYGAALGFL--AVWHGYHSGYYMTFL 369
BLAST of MBOAT2 vs. Ensembl Zebrafish
Match: mboat2b (membrane bound O-acyltransferase domain containing 2b [Source:ZFIN;Acc:ZDB-GENE-041001-185]) HSP 1 Score: 276.944 bits (707), Expect = 3.483e-84 Identity = 160/461 (34.71%), Postives = 247/461 (53.58%), Query Frame = 2
Query: 80 SGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLV-RQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTP 1453
+GS + QP+S+ L +DQ NF+ CQL A F YL+ K SP RH++ + G+ +FCFG H L QS Y +M + + L L +LS + R+Y +DYG Y+ D TGP+M+ TQK++S+AF +HDG +K E L + + A+ + P+LLE+FSY F GI+ GP DY+ FIEG D E + + K++ + E+ + +C + L+ + V F + D+++ + FY ++ +S L R KYYF W++ N +G GF+G+ + P +D++ N IEL TS K +DNWN+ T +WL+ Y+R P T A F+ S++WHG YPGYYL F++ + T A+RA R +RP + S YDV+T +A+ Y +PF L V + + +F +H+G +LL P
Sbjct: 10 TGSNLLQPISEMTNLPLDQVNFVVCQLCALLTAVWFRLYLHPSKTSPFVRHVVATLLGIYFALFCFGWYALHFLLQSGITYGIMILTGVEHMHKYCLVVALGYLSFCQITRVYVFDYGMYSADFTGPMMVITQKITSVAFEIHDGMA-RKVEQLNQG-QKLLAIRRMPSLLEYFSYNCNFMGILAGPTCSYNDYIAFIEGTPYRHRDLEPKGKVNGKSRLNDPSPNTEVIRKLSTCFISLLVFLSVCKVFPVERNVDDDFIATSPFYVQVVYLYLSMLTTRPKYYFVWTLADAINNAAGFGFNGYDSDGSPRWDLLSNIRILNIELATSFKVFLDNWNIQTAHWLKRVCYERCPYN-PTAATFLLSAMWHGAYPGYYLTFLTGIVITLAARAVRHNVRPHFLGSPSHKFVYDVITWAATQIAICYTVVPFVLLSVGPSLKFYRSWYFCLHIGCILLAIALP 467
BLAST of MBOAT2 vs. Ensembl Zebrafish
Match: mboat2a (membrane bound O-acyltransferase domain containing 2a [Source:ZFIN;Acc:ZDB-GENE-041210-257]) HSP 1 Score: 275.789 bits (704), Expect = 1.140e-83 Identity = 165/466 (35.41%), Postives = 249/466 (53.43%), Query Frame = 2
Query: 80 SGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGR---QEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPH--FDPDYFKTDEYLQSGMFYKYIMLTIVSFL-VRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTP 1453
+GS + QP+S + L +DQ NF+ CQL A F F YL+ K SP RH++ + G L +FCFG H L QS Y +M S + + + +LS H+ R+Y +DYG Y+ D TGP+M+ TQK++SLAF +HDG +K+ E L+ + A+ K P+LLE+ SY F GI+ GP DY+ FIEGR + + N KE F++ + + P+ K C LTI++ H + + ++ S FY ++ +S L +R KYYF W++ N +G GF+G++ + P +D + N IE TS K +DNWN+ T WL+ Y+R P T A F+ S++WHG YPGYYL F++ T A+RA R +R + ++L L YDV+T +A+ Y +PF L V + + + +H+ VLL + P
Sbjct: 8 TGSTLLQPISKMIDLPLDQVNFVVCQLFALVTAFWFRLYLHPSKTSPFIRHVVATLLGFYLALFCFGWYALHFLVQSGLAYGVMIFTSVEHMHKYCFVVTMGYLSFCHVTRVYVFDYGMYSADFTGPMMVITQKITSLAFEIHDG-LVKREEHLKPS-QKYLAVRKMPSLLEYLSYNCNFMGILAGPTCSYNDYIDFIEGRGYESKHLETNGKENGKFKQIEPS-----PKRDVIQKVCTCALCLTIYLVIHTACPTERVTHESFMASNPFYIQVLFLYISMLSLRPKYYFVWTLADAINNAAGFGFNGYNTDGTPKWDRISNLRILNIEFATSFKMFLDNWNIQTALWLKRVCYERCPYN-PTAATFLLSAMWHGVYPGYYLTFVTGIAVTMAARAVRHNVRQHFLGSSTLKLIYDVITWFSTQIAICYTVVPFVLLAVGPSIKFYRSWYCCIHLICVLLALVLP 465
BLAST of MBOAT2 vs. Ensembl Zebrafish
Match: mboat2b (membrane bound O-acyltransferase domain containing 2b [Source:ZFIN;Acc:ZDB-GENE-041001-185]) HSP 1 Score: 265.003 bits (676), Expect = 1.592e-79 Identity = 157/442 (35.52%), Postives = 238/442 (53.85%), Query Frame = 2
Query: 80 SGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLV-RQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEV--QLTFY 1390
+GS + QP+S+ L +DQ NF+ CQL A F YL+ K SP RH++ + G+ +FCFG H L QS Y +M + + L L +LS + R+Y +DYG Y+ D TGP+M+ TQK++S+AF +HDG +K E L + + A+ + P+LLE+FSY F GI+ GP DY+ FIEG D E + + K++ + E+ + +C + L+ + V F + D+++ + FY ++ +S L R KYYF W++ N +G GF+G+ + P +D++ N IEL TS K +DNWN+ T +WL+ Y+R P T A F+ S++WHG YPGYYL F++ + T A+RA R +RP + S YDV+T +A+ Y +PF L V L FY
Sbjct: 80 TGSNLLQPISEMTNLPLDQVNFVVCQLCALLTAVWFRLYLHPSKTSPFVRHVVATLLGIYFALFCFGWYALHFLLQSGITYGIMILTGVEHMHKYCLVVALGYLSFCQITRVYVFDYGMYSADFTGPMMVITQKITSVAFEIHDGMA-RKVEQLNQG-QKLLAIRRMPSLLEYFSYNCNFMGILAGPTCSYNDYIAFIEGTPYRHRDLEPKGKVNGKSRLNDPSPNTEVIRKLSTCFISLLVFLSVCKVFPVERNVDDDFIATSPFYVQVVYLYLSMLTTRPKYYFVWTLADAINNAAGFGFNGYDSDGSPRWDLLSNIRILNIELATSFKVFLDNWNIQTAHWLKRVCYERCPYN-PTAATFLLSAMWHGAYPGYYLTFLTGIVITLAARAVRHNVRPHFLGSPSHKFVYDVITWAATQIAICYTVVPFVLLSVGPSLKFY 518
BLAST of MBOAT2 vs. Ensembl Zebrafish
Match: mboat1 (membrane bound O-acyltransferase domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-041114-98]) HSP 1 Score: 245.358 bits (625), Expect = 2.013e-72 Identity = 158/481 (32.85%), Postives = 247/481 (51.35%), Query Frame = 2
Query: 80 SGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEG-----RQEEVLDNEKEKEYFRK--NKSTILAGWPEIGFYVKSCLVFGLLT--IFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLAPLFSSASGD 1489
+G PVSD VG+ +DQ NF+ CQL F F +L+ + +P RH + + G S +FCFG H+ ++ Y +M S ++ + + + +L+ ++R++ +DYG + D +GPLM+ TQK++SLAF ++DG K+ E +D R+ A+ P+LLE+ SY F ++VGP DY FI+G R + V D ++ + K S + ++ V L F ++T ++ + DP F T+ S + Y ++ + R K+YFAW++ + +G G SG +E + +D+V N ++IE TS KS IDNWN+ T WL+ YDR P Y T FV S+IWHG YPGYY F++ T A+R+ R+ R + L YDV+T L + Y +PF L ++ T + M+F VHV S+L L P F + D
Sbjct: 14 TGCTWLSPVSDQVGVPLDQVNFIACQLFGLAAAFWFRLFLSPQRATPEVRHAVAAILGASSVMFCFGWYAVHIFILVLACYGIMIKASVRNVHRYTMVASIGYLTACQVSRVFIFDYGILSTDFSGPLMMITQKITSLAFQVYDGMGRKQEE--LKDDQRQLAVHSKPSLLEYLSYNINFLSVLVGPCSNYKDYEEFIKGQHVQHRLKRVKDGSLQQNGWDKVPEPSPVRTVITKVLVCVCCLLWFFIITRAFPIKYNVDPQ-FITEASFISRLAYAFVSIQAA----RPKFYFAWTLADAVHNAAGYGVSGVNERGEVSWDLVSNVRIWEIETATSFKSFIDNWNIQTGLWLKRVCYDRAP-FYRTPLTFVLSAIWHGVYPGYYFTFITAIPITLAARSVRRNFRKYFLSSRQLKACYDVVTWGATQLTICYTVMPFLLLALEPTLKYYRSMYFHVHVISILAMILLPGKPKSFKPENHD 486
BLAST of MBOAT2 vs. Ensembl Zebrafish
Match: mboat1 (membrane bound O-acyltransferase domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-041114-98]) HSP 1 Score: 180.644 bits (457), Expect = 6.401e-50 Identity = 114/355 (32.11%), Postives = 186/355 (52.39%), Query Frame = 2
Query: 80 SGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEG-----RQEEVLDNEKEKEYFRK--NKSTILAGWPEIGFYVKSCLVFGLLTIF--VQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRE 1114
+G PVSD VG+ +DQ NF+ CQL F F YL+ + +P RH + + G S +FCFG H+ ++ Y +M S ++ + + + +L+ ++R++ +DYG + D +GPLM+ TQK++SLAF ++DG +K E L+ D R+ A+ P+LLE+ SY F ++VGP DY FI+G R + V D ++ + K S + ++ V L F ++T ++ + DP F T+ S + Y ++ + R K+YFAW++ + +G G SG +E + +D+V N ++IE TS KS IDNWN+ T WL+
Sbjct: 14 TGCTWLSPVSDQVGVPLDQVNFIACQLFGLAAAFWFRLYLSPQRATPEVRHAVAAILGASSVMFCFGWYAVHIFILVLACYGIMIKASVRNVHRYTMVASIGYLTACQVSRVFIFDYGILSTDFSGPLMMITQKITSLAFQVYDGMG-RKQEELKDD-QRRLAVHSKPSLLEYLSYNINFLSVLVGPCSNYKDYEEFIKGQHVQHRLKRVKDGSLQQNGWDKVPEPSPVRTVITKVLVCVCCLLWFFIITRAFPIKYNVDPQ-FITEASFISRLAYAFVSIQ----AARPKFYFAWTLADAVHNAAGYGVSGVNERGEVSWDLVSNVRIWEIETATSFKSFIDNWNIQTGLWLKR 361
BLAST of MBOAT2 vs. Ensembl Xenopus
Match: ahcyl1 (adenosylhomocysteinase like 1 [Source:Xenbase;Acc:XB-GENE-942022]) HSP 1 Score: 268.47 bits (685), Expect = 1.265e-80 Identity = 162/481 (33.68%), Postives = 250/481 (51.98%), Query Frame = 2
Query: 65 MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVL--------DNEKEKEYFRKNKSTI---LAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQ--SGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIR-PLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLA 1462
M+ +GS + P+S AV L VDQ NF+ CQ+ A F YL++ K SP RH++ + G+ L +FCFG H + QS Y +M A ++ F F L +L++ +AR+Y +DYG Y+ D +GP+MI TQK++SLAF + DG K+ E R+ A+ + P+LLE+ SY F GI+ GP DY+ FIEGR ++ D + E++ N + I + + FY+ + + P + DE Q S + + I L + R KYYFAW++ N +G GF G+ + + +D+V N + +IE TS K IDNWN+ T WL+ Y+R + T F+ S+IWHG YPGYY+ F + +L T A+R R R +H + L YDV+T + +A++Y PF L V+ + +S ++ +H+ +L+ P A
Sbjct: 1 MATSTTGSTLLLPLSRAVQLPVDQVNFVACQVFALLAAIWFRIYLHSSKTSPLIRHVVATLLGLYLALFCFGWYALHFIVQSGIAYYIMIAGRLENMHKFCFVFALGYLTLCQIARVYIFDYGQYSADFSGPMMIMTQKITSLAFEIRDGIFCKEEE--LTPSQRRLAVRRMPSLLEYLSYNCNFMGILAGPLCSYKDYIAFIEGRSYQLKQFEANGKEDPKHEQKEPCPNSAVIHKVIVSAVSLAFYMTATRIL------------PVEYNIDEQFQETSSVLNRIIYLYLSLMAARPKYYFAWTLADAINNAAGFGFQGYDKNGEENWDLVSNLNIKQIEFSTSFKMFIDNWNIQTALWLKRVCYERATYS-ATAKTFLLSAIWHGVYPGYYMTFFTGSLMTLAARRIRNNARHHFLHSSELMFVYDVITWIATQVAISYTVAPFVLLSVKPSLQFYSFWYYCLHIACILVLLAMPARA 466
BLAST of MBOAT2 vs. Ensembl Xenopus
Match: ahcyl1 (adenosylhomocysteinase like 1 [Source:Xenbase;Acc:XB-GENE-942022]) HSP 1 Score: 231.876 bits (590), Expect = 4.504e-67 Identity = 156/481 (32.43%), Postives = 243/481 (50.52%), Query Frame = 2
Query: 65 MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVL--------DNEKEKEYFRKNKSTI---LAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQ--SGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIR-PLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLA 1462
M+ +GS + P+S AV L VDQ NF+ CQ+ A F YL++ K SP RH++ + G+ L +FCFG H + QS Y +M A ++ F F L +L++ +AR+Y +DYG Y+ D +GP+MI TQK++SLAF + DG K+ E R+ A+ + P+LLE+ SY F GI+ GP DY+ FIEGR ++ D + E++ N + I + + FY+ + + P + DE Q S + + I L + R KYYFAW++ N +G GF G+ + + +D+V N + K L S + + Y LR C Y+R + T F+ S+IWHG YPGYY+ F + +L T A+R R R +H + L YDV+T + +A++Y PF L V+ + +S ++ +H+ +L+ P A
Sbjct: 1 MATSTTGSTLLLPLSRAVQLPVDQVNFVACQVFALLAAIWFRIYLHSSKTSPLIRHVVATLLGLYLALFCFGWYALHFIVQSGIAYYIMIAGRLENMHKFCFVFALGYLTLCQIARVYIFDYGQYSADFSGPMMIMTQKITSLAFEIRDGIFCKEEE--LTPSQRRLAVRRMPSLLEYLSYNCNFMGILAGPLCSYKDYIAFIEGRSYQLKQFEANGKEDPKHEQKEPCPNSAVIHKVIVSAVSLAFYMTATRIL------------PVEYNIDEQFQETSSVLNRIIYLYLSLMAARPKYYFAWTLADAINNAAGFGFQGYDKNGEENWDLVSNLN-IKYLLYISLI-------LCSFYSLRVC-YERATYS-ATAKTFLLSAIWHGVYPGYYMTFFTGSLMTLAARRIRNNARHHFLHSSELMFVYDVITWIATQVAISYTVAPFVLLSVKPSLQFYSFWYYCLHIACILVLLAMPARA 457
BLAST of MBOAT2 vs. Ensembl Xenopus
Match: kcna4 (potassium voltage-gated channel subfamily A member 4 [Source:Xenbase;Acc:XB-GENE-492827]) HSP 1 Score: 227.254 bits (578), Expect = 1.742e-65 Identity = 144/446 (32.29%), Postives = 220/446 (49.33%), Query Frame = 2
Query: 134 QFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQ-EEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQ--PHFDPDYFKTDEYLQSGMFYKYI-MLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTP 1453
Q NF+ CQLVA F F YLN P RH GV +FCFG H+ + Y +M S + + + +L++ + R+Y ++YG + D +GPLMI TQK+++LAF LHDG + + L +D +++++ K P+ LE+ SY F ++ GP DY+ FIEGR + L N K Y + + P K C+ L +F+ F Y + ++ + F K + I + + KYYFAW++ N +G GF+G E+ K +D++ N + + IE TS K IDNWN+ T WL+ YDR P+ Y T F+ S +WHG YPGYY F++ A+RA R R AS YD++T LA+ Y PF L + ++ ++F +H+ L+ + P
Sbjct: 25 QVNFVACQLVALLVAFWFRIYLNPSNAHPAVRHAFATFVGVYFAVFCFGWYSLHIFTLVLLCYCIMITASVSNVHRYSFIMAMGYLTLCQINRVYIFNYGILSTDFSGPLMIITQKITTLAFQLHDGFG-RSFKVLSKD-QQQYSIKKKPSFLEYLSYHLNFMSVLAGPCSNFQDYIAFIEGRHIQSKLLNSKGNGYTKLPNPS-----PNEVVIYKLCIAAASLAVFMTFTKAFPILYVVDETFMNTVPFLKRLGYFYIATQACKPKYYFAWTLADAVNNAAGYGFNGVDEKGKFRWDLISNLNIWNIETATSFKMYIDNWNIQTAAWLKRVCYDRAPK-YRTGLTFILSGVWHGVYPGYYFTFVTAIPVMLAARAMRNNFRHYFTTSASWKFLYDIVTWTATQLAICYTVAPFVLLAAGPSVQLYKSLYFYLHIVCFLVLIILP 462
BLAST of MBOAT2 vs. Ensembl Xenopus
Match: kcna4 (potassium voltage-gated channel subfamily A member 4 [Source:Xenbase;Acc:XB-GENE-492827]) HSP 1 Score: 217.624 bits (553), Expect = 1.505e-62 Identity = 146/435 (33.56%), Postives = 209/435 (48.05%), Query Frame = 2
Query: 119 GLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQ-EEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQ--PHFDPDYFKTDEYLQSGMFYKYI-MLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQL----EVQLTFYI 1393
L + Q NF+ CQLVA F F YLN P RH GV +FCFG H+ + Y +M S + + + +L++ + R+Y ++YG + D +GPLMI TQK+++LAF LHDG+ + R Y RK P+ LE+ SY F ++ GP DY+ FIEGR + L N K Y + + P K C+ L +F+ F Y + ++ + F K + I + + KYYFAW++ N +G GF+G E+ K +D++ N + + IE TS K IDNWN+ T WL+ YDR P+ Y T F+ S +WHG YPGYY F++ A+RA R R AS YD++T LA+ Y PF L VQL Y+
Sbjct: 14 SLLLLQVNFVACQLVALLVAFWFRIYLNPSNAHPAVRHAFATFVGVYFAVFCFGWYSLHIFTLVLLCYCIMITASVSNVHRYSFIMAMGYLTLCQINRVYIFNYGILSTDFSGPLMIITQKITTLAFQLHDGKLL---HLFIRLYVRKK-----PSFLEYLSYHLNFMSVLAGPCSNFQDYIAFIEGRHIQSKLLNSKGNGYTKLPNPS-----PNEVVIYKLCIAAASLAVFMTFTKAFPILYVVDETFMNTVPFLKRLGYFYIATQACKPKYYFAWTLADAVNNAAGYGFNGVDEKGKFRWDLISNLNIWNIETATSFKMYIDNWNIQTAAWLKRVCYDRAPK-YRTGLTFILSGVWHGVYPGYYFTFVTAIPVMLAARAMRNNFRHYFTTSASWKFLYDIVTWTATQLAICYTVAPFVLLAAGPSVQLYKYV 434
BLAST of MBOAT2 vs. Ensembl Xenopus
Match: kcna4 (potassium voltage-gated channel subfamily A member 4 [Source:Xenbase;Acc:XB-GENE-492827]) HSP 1 Score: 216.083 bits (549), Expect = 5.278e-62 Identity = 146/443 (32.96%), Postives = 213/443 (48.08%), Query Frame = 2
Query: 98 QPVSDAVGLTVDQF-NFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQ-EEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQ--PHFDPDYFKTDEYLQSGMFYKYI-MLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQL----EVQLTFYI 1393
+ + +GL + + NF+ CQLVA F F YLN P RH GV +FCFG H+ + Y +M S + + + +L++ + R+Y ++YG + D +GPLMI TQK+++LAF LHDG+ + R Y RK P+ LE+ SY F ++ GP DY+ FIEGR + L N K Y + + P K C+ L +F+ F Y + ++ + F K + I + + KYYFAW++ N +G GF+G E+ K +D++ N + + IE TS K IDNWN+ T WL+ YDR P+ Y T F+ S +WHG YPGYY F++ A+RA R R AS YD++T LA+ Y PF L VQL Y+
Sbjct: 4 RQIRSTLGLPSNVYVNFVACQLVALLVAFWFRIYLNPSNAHPAVRHAFATFVGVYFAVFCFGWYSLHIFTLVLLCYCIMITASVSNVHRYSFIMAMGYLTLCQINRVYIFNYGILSTDFSGPLMIITQKITTLAFQLHDGKLL---HLFIRLYVRKK-----PSFLEYLSYHLNFMSVLAGPCSNFQDYIAFIEGRHIQSKLLNSKGNGYTKLPNPS-----PNEVVIYKLCIAAASLAVFMTFTKAFPILYVVDETFMNTVPFLKRLGYFYIATQACKPKYYFAWTLADAVNNAAGYGFNGVDEKGKFRWDLISNLNIWNIETATSFKMYIDNWNIQTAAWLKRVCYDRAPK-YRTGLTFILSGVWHGVYPGYYFTFVTAIPVMLAARAMRNNFRHYFTTSASWKFLYDIVTWTATQLAICYTVAPFVLLAAGPSVQLYKYV 432
BLAST of MBOAT2 vs. Ensembl Mouse
Match: Mboat2 (membrane bound O-acyltransferase domain containing 2 [Source:MGI Symbol;Acc:MGI:1914466]) HSP 1 Score: 266.929 bits (681), Expect = 3.237e-80 Identity = 164/461 (35.57%), Postives = 248/461 (53.80%), Query Frame = 2
Query: 98 QPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVL---DNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIF--VQPHFDPDYFKTDEYLQSGMFY--KYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTP 1453
QP+S+AV L +DQ NF+ CQL A F YL++ K S RH++ + G+ L FCFG H L QS Y +M +S + F L +LSV + R+Y +DYG Y+ D +GP+MI TQK++SLA+ +HDG +K+E L R A+ + P+LLE+ SY F GI+ GP DY+ FIEGR V +N K++++ + + S A + LV GL +F + P + DE+ Q+ + K L + R KYYFAW++ N +G GF G+ + +D++ N +IE+ TS K +DNWN+ T WL+ Y+R + T+ F S+IWHG YPGYYL F++ L T A+RA R R + L L YD++T + + ++Y +PF L ++ +F +S ++ +HV S+L+ L P
Sbjct: 13 QPLSNAVQLPIDQVNFVVCQLFALLAAVWFRTYLHSSKTSSFIRHVVATLLGLYLAFFCFGWYALHFLVQSGISYCIMIIAGVESMQQCCFVFALGYLSVCQITRVYIFDYGQYSADFSGPMMIITQKITSLAYEIHDGM-FRKDEELTPS-QRGLAVRRMPSLLEYVSYTCNFMGILAGPLCSYKDYIAFIEGRASHVAQPSENGKDEQHGKADPSPNAA-------VTEKLLVCGLSLLFHLTISNMLPVEYNIDEHFQATASWPTKATYLYVSLLAARPKYYFAWTLADAINNAAGFGFRGYDKNGVARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRVCYERATFS-PTIQTFFLSAIWHGVYPGYYLTFLTGVLMTLAARAVRNNFRHYFLEPPQLKLFYDLITWVATQITISYTVVPFVLLSIKPSFTFYSSWYYCLHVCSILVLLLLP 463
BLAST of MBOAT2 vs. Ensembl Mouse
Match: Mboat1 (membrane bound O-acyltransferase domain containing 1 [Source:MGI Symbol;Acc:MGI:2387184]) HSP 1 Score: 243.432 bits (620), Expect = 1.023e-71 Identity = 149/453 (32.89%), Postives = 235/453 (51.88%), Query Frame = 2
Query: 80 SGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTI-------FVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGR-KYIRPLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFF 1411
+GS P+S +G+ +DQ NF+ CQL A F F YL+ K SP RH + + G+ +FCFG HL + Y +M S + + + +L++ H++R+Y + YG T D +GPLMI TQK+++LAF +HDG +K E L + HR A+ P+LLE+ SY F ++ GP DY+ FIEGR + E + ++ ++ P K C+ L + F D+F S ++Y Y+++ + KYYFAW++ + +G GF+G + K +D++ N + +KIE TS K ++NWN+ T WL+ Y+R+P Y T+ F+ S++WHG YPGYY F++ T A+RA R Y + + + YDV+T + LA++Y A PF L V+ T ++ +FF
Sbjct: 14 TGSTCLHPLSQLLGIPLDQVNFVACQLFALSAAFWFRIYLHPGKASPEVRHTLATILGIYFVVFCFGWYAVHLFVLVLMCYGVMVTASVSNIHRYSFFVAMGYLTICHISRIYIFHYGILTTDFSGPLMIVTQKITTLAFQVHDGLG-RKAEDLSAEQHRL-AVKAKPSLLEYLSYHLNFMSVIAGPCNNFKDYVAFIEGRHIHM--KLLEVNWTQRGFQSLPEPSPMGAVIQKLCVTLMSLLLFLTLSKSFPVTFLIDDWFVHKANFLSRLWYLYVVMQA----AKPKYYFAWTLADAVHNAAGFGFNGMDTDGKSRWDLLSNLNIWKIETATSFKMYLENWNIQTSTWLKCVCYERVPW-YPTVLTFLLSALWHGVYPGYYFTFLTGVPVTLAARAVRNNYRHHFLSSKARKIAYDVVTWAVTQLAVSYTAAPFVMLAVEPTISLYKSVFF 457
BLAST of MBOAT2 vs. Ensembl Mouse
Match: Mboat2 (membrane bound O-acyltransferase domain containing 2 [Source:MGI Symbol;Acc:MGI:1914466]) HSP 1 Score: 230.335 bits (586), Expect = 6.097e-67 Identity = 151/460 (32.83%), Postives = 230/460 (50.00%), Query Frame = 2
Query: 98 QPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVL---DNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIF--VQPHFDPDYFKTDEYLQSGMFY--KYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTP 1453
QP+S+AV L +DQ NF+ CQL A F YL++ K S RH++ + G+ L FCFG H L QS Y +M +S ++ P+MI TQK++SLA+ +HDG +K+E L R A+ + P+LLE+ SY F GI+ GP DY+ FIEGR V +N K++++ + + S A + LV GL +F + P + DE+ Q+ + K L + R KYYFAW++ N +G GF G+ + +D++ N +IE+ TS K +DNWN+ T WL+ Y+R + T+ F S+IWHG YPGYYL F++ L T A+RA R R + L L YD++T + + ++Y +PF L ++ +F +S ++ +HV S+L+ L P
Sbjct: 13 QPLSNAVQLPIDQVNFVVCQLFALLAAVWFRTYLHSSKTSSFIRHVVATLLGLYLAFFCFGWYALHFLVQSGISYCIMIIAGVESMQH-------------------------------PMMIITQKITSLAYEIHDGM-FRKDEELTPS-QRGLAVRRMPSLLEYVSYTCNFMGILAGPLCSYKDYIAFIEGRASHVAQPSENGKDEQHGKADPSPNAA-------VTEKLLVCGLSLLFHLTISNMLPVEYNIDEHFQATASWPTKATYLYVSLLAARPKYYFAWTLADAINNAAGFGFRGYDKNGVARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRVCYERATFS-PTIQTFFLSAIWHGVYPGYYLTFLTGVLMTLAARAVRNNFRHYFLEPPQLKLFYDLITWVATQITISYTVVPFVLLSIKPSFTFYSSWYYCLHVCSILVLLLLP 431
BLAST of MBOAT2 vs. Ensembl Mouse
Match: Mboat2 (membrane bound O-acyltransferase domain containing 2 [Source:MGI Symbol;Acc:MGI:1914466]) HSP 1 Score: 185.267 bits (469), Expect = 1.753e-51 Identity = 116/340 (34.12%), Postives = 181/340 (53.24%), Query Frame = 2
Query: 458 LMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVL---DNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIF--VQPHFDPDYFKTDEYLQSGMFY--KYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTP 1453
+MI TQK++SLA+ +HDG +K+E L R A+ + P+LLE+ SY F GI+ GP DY+ FIEGR V +N K++++ + + S A + LV GL +F + P + DE+ Q+ + K L + R KYYFAW++ N +G GF G+ + +D++ N +IE+ TS K +DNWN+ T WL+ Y+R + T+ F S+IWHG YPGYYL F++ L T A+RA R R + L L YD++T + + ++Y +PF L ++ +F +S ++ +HV S+L+ L P
Sbjct: 1 MMIITQKITSLAYEIHDGM-FRKDEELTPS-QRGLAVRRMPSLLEYVSYTCNFMGILAGPLCSYKDYIAFIEGRASHVAQPSENGKDEQHGKADPSPNAA-------VTEKLLVCGLSLLFHLTISNMLPVEYNIDEHFQATASWPTKATYLYVSLLAARPKYYFAWTLADAINNAAGFGFRGYDKNGVARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRVCYERATFS-PTIQTFFLSAIWHGVYPGYYLTFLTGVLMTLAARAVRNNFRHYFLEPPQLKLFYDLITWVATQITISYTVVPFVLLSIKPSFTFYSSWYYCLHVCSILVLLLLP 330
BLAST of MBOAT2 vs. Ensembl Mouse
Match: Lpcat3 (lysophosphatidylcholine acyltransferase 3 [Source:MGI Symbol;Acc:MGI:1315211]) HSP 1 Score: 98.2117 bits (243), Expect = 5.946e-21 Identity = 100/422 (23.70%), Postives = 177/422 (41.94%), Query Frame = 2
Query: 230 HIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMS-TKSARYFILGFVLLFLSVAHLARMYYDY-GGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRI------PRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIR------PLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTP 1453
H+ G+S+ F FG Q +H L + ++++ M T +A L F + +L LA YY G Y + T P + T KL L + +DG K L + +K+A+ P+LLE + +++ +VGP + Y++ + G+ ++ + STI P + + LV+ + + PH DY T++Y +++ + + I V KY W + I SGLGF+GF E +D N + E I ++N+ T W+ ++ R+ + G +F+ ++WHG + GY +CF L + IR L +L Y ++ + L + Y F ++ ++F+ HV + L F+ P
Sbjct: 71 HLFHTFTGLSIAYFNFGHQFYHSLLCVVLQFLILRLMGRTVTAVITTLCFQMAYL----LAGYYYTATGDYDIKWTMPHCVLTLKLIGLCIDYYDGG--KDGNSLTSE-QQKYAIRGVPSLLEVAGFSYFYGAFLVGPQFSMNHYMKLVRGQLTDIPG--------KMPNSTI----PALK-RLSLGLVYLVGYTLLSPHITDDYLLTEDYDNRPFWFRCMYMLIWGKFVLYKYVTCWLVTEGVCILSGLGFNGFDENGTVRWDACANMKVWLFETTPRFNGTIASFNINTNAWVARYIFKRLKFLGNKELSQGLSLLFL--ALWHGLHSGYLICFQMEFLIVIVEKQVSSLIRDSPALSSLASITALQPFYYLVQQTIHWLFMGYSMTAFCLFTWDKWLKVYRSIYFLGHVFFLSLLFILP 470
BLAST of MBOAT2 vs. UniProt/SwissProt
Match: sp|Q5ZKL6|MBOA2_CHICK (Membrane-bound O-acyltransferase domain-containing protein 2 OS=Gallus gallus OX=9031 GN=mboat2 PE=2 SV=1) HSP 1 Score: 285.804 bits (730), Expect = 1.956e-86 Identity = 170/478 (35.56%), Postives = 263/478 (55.02%), Query Frame = 2
Query: 65 MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGR----QEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIF---------VQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTP 1453
M+ +GS + QP+S+AV L VDQ NF+ CQL A F YL++ K SP RH++ + G+ L +FCFG H + QS Y +M + ++ + F L +L+V + R+Y +DYG Y+ D +GP+MI TQK++SLAF +HDG +KNE L R A+ + P+LLE+ SY F GI+ GP DY+ FIEGR Q+ + +++ +Y + + S P I + L+ GL +F V+ + D ++ T + +FY Y+ L R KYYFAW++ N +G GF G+ + +D++ N +IE TS K +DNWN+ T WL+ Y+R + T+ F+ S+IWHG YPGYYL F++ L T A+RA R IR V ++ L YD++T + +A++Y +PF L V+ +F +S +F +H+ S+L+ + P
Sbjct: 1 MATSTTGSTLLQPLSNAVRLPVDQVNFVVCQLFALLAAIWFRTYLHSSKTSPFIRHVVATLLGLYLALFCFGWYALHFVIQSGISYYLMIIIGVENMHKYCFVFALGYLTVCQITRVYIFDYGQYSADFSGPMMIITQKITSLAFEIHDGM-FRKNEDLTPS-QRCLAVRRMPSLLEYLSYNCNFMGILAGPLCSYKDYITFIEGRSYQLQQSEANGKEDTKYEQTDPS------PNIAV-AQKLLICGLSLLFHMTITKTLPVEYNIDENFRATASW-PVRVFYLYLSL----MAARPKYYFAWTLADAINNAAGFGFRGYDKNGVTRWDLISNLRIQQIESSTSFKMFLDNWNIQTALWLKRVCYERATFS-PTIQTFILSAIWHGVYPGYYLTFLTGVLMTLAARAIRNNIRHYFVESPAVKLCYDIITWMTTQVAISYTVVPFVLLSVKPSFTFYSSCYFCLHIASILVLLVFP 463
BLAST of MBOAT2 vs. UniProt/SwissProt
Match: sp|Q6ZWT7|MBOA2_HUMAN (Lysophospholipid acyltransferase 2 OS=Homo sapiens OX=9606 GN=MBOAT2 PE=2 SV=2) HSP 1 Score: 270.781 bits (691), Expect = 1.023e-80 Identity = 164/445 (36.85%), Postives = 243/445 (54.61%), Query Frame = 2
Query: 98 QPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVL---DNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFD-----PDYFKTDEYLQSGMFY--KYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQ--LTFY 1390
QP+S+AV L +DQ NF+ CQL A F YL++ K S RH++ + G+ L +FCFG H L QS Y +M + ++ + F L +L+V + R+Y +DYG Y+ D +GP+MI TQK++SLA +HDG +K+E L R A+ + P+LLE+ SY F GI+ GP DY+ FIEGR + +N KE+ + + + + P V+ LV GL +F H P + DE+ Q+ + K I L I R KYYFAW++ N +G GF G+ E +D++ N +IE+ TS K +DNWN+ T WL+ Y+R + T+ F+ S+IWHG YPGYYL F++ L T A+RA R R + + L L YDV+T ++ +A++Y +PF L ++ LTFY
Sbjct: 13 QPLSNAVQLPIDQVNFVVCQLFALLAAIWFRTYLHSSKTSSFIRHVVATLLGLYLALFCFGWYALHFLVQSGISYCIMIIIGVENMHNYCFVFALGYLTVCQVTRVYIFDYGQYSADFSGPMMIITQKITSLACEIHDGM-FRKDEELTSS-QRDLAVRRMPSLLEYLSYNCNFMGILAGPLCSYKDYITFIEGRSYHITQSGENGKEETQYERTEPS-----PNTAV-VQKLLVCGLSLLF---HLTICTTLPVEYNIDEHFQATASWPTKIIYLYISLLAARPKYYFAWTLADAINNAAGFGFRGYDENGAARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRVCYERTSFS-PTIQTFILSAIWHGVYPGYYLTFLTGVLMTLAARAMRNNFRHYFIEPSQLKLFYDVITWIVTQVAISYTVVPFVLLSIKPSLTFY 445
BLAST of MBOAT2 vs. UniProt/SwissProt
Match: sp|Q3T1J2|MBOA2_RAT (Membrane-bound O-acyltransferase domain-containing protein 2 OS=Rattus norvegicus OX=10116 GN=Mboat2 PE=2 SV=2) HSP 1 Score: 267.7 bits (683), Expect = 1.346e-79 Identity = 169/490 (34.49%), Postives = 253/490 (51.63%), Query Frame = 2
Query: 98 QPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVL---DNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIF--VQPHFDPDYFKTDEYLQSGMFY--KYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTP-KLAPLFSSASGDHSPRVADKISGDDISV 1537
QP+S+AV L +DQ NF+ CQL A F YL++ K S RH++ + G+ L FCFG H L QS Y +M +S F L +LSV + R+Y +DYG Y+ D +GP+MI TQK++SLA+ +HDG +K+E L R A+ + P+LLE+ SY F GI+ GP DY+ FIEGR + +N KE+++ + S A + LV GL +F P + DE+ Q+ + K L + R KYYFAW++ N +G GF G+ + +D++ N +IE+ TS K +DNWN+ T WL+ Y+R + T+ F S+IWHG YPGYYL F++ L T A+RA R R V L L YD++T + ++Y +PF L + +F + ++ +H+ S+L+ L P K +P + + P A K + S+
Sbjct: 13 QPLSNAVQLPIDQVNFVVCQLFALLAAVWFRTYLHSSKTSSFIRHVVATLLGLYLAFFCFGWYALHFLVQSGISYCIMIIAGVESMHQCCFVFALGYLSVCQITRVYIFDYGQYSADFSGPMMIITQKITSLAYEIHDGM-FRKDEELTPS-QRGLAVRRMPSLLEYVSYTCNFMGILAGPLCSYKDYIAFIEGRASHMAQSGENGKEEQHGKAEPSPNAA-------VTEKLLVCGLSLLFHLTISSMLPVEYNIDEHFQATASWPTKATYLYVSLLAARPKYYFAWTLADAINNAAGFGFRGYDKNGVARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRVCYERATFS-PTVQTFFLSAIWHGVYPGYYLTFLTGVLMTLAARAVRNNFRHYFVEPPQLKLFYDIITWAATQITISYTVVPFVLLSINPSFTFYRSWYYCLHICSILVLLLLPVKKSPRKKNTEENAQPSWAKKFDERENSL 492
BLAST of MBOAT2 vs. UniProt/SwissProt
Match: sp|Q8R3I2|MBOA2_MOUSE (Lysophospholipid acyltransferase 2 OS=Mus musculus OX=10090 GN=Mboat2 PE=1 SV=1) HSP 1 Score: 266.929 bits (681), Expect = 2.264e-79 Identity = 164/461 (35.57%), Postives = 248/461 (53.80%), Query Frame = 2
Query: 98 QPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVL---DNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIF--VQPHFDPDYFKTDEYLQSGMFY--KYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTP 1453
QP+S+AV L +DQ NF+ CQL A F YL++ K S RH++ + G+ L FCFG H L QS Y +M +S + F L +LSV + R+Y +DYG Y+ D +GP+MI TQK++SLA+ +HDG +K+E L R A+ + P+LLE+ SY F GI+ GP DY+ FIEGR V +N K++++ + + S A + LV GL +F + P + DE+ Q+ + K L + R KYYFAW++ N +G GF G+ + +D++ N +IE+ TS K +DNWN+ T WL+ Y+R + T+ F S+IWHG YPGYYL F++ L T A+RA R R + L L YD++T + + ++Y +PF L ++ +F +S ++ +HV S+L+ L P
Sbjct: 13 QPLSNAVQLPIDQVNFVVCQLFALLAAVWFRTYLHSSKTSSFIRHVVATLLGLYLAFFCFGWYALHFLVQSGISYCIMIIAGVESMQQCCFVFALGYLSVCQITRVYIFDYGQYSADFSGPMMIITQKITSLAYEIHDGM-FRKDEELTPS-QRGLAVRRMPSLLEYVSYTCNFMGILAGPLCSYKDYIAFIEGRASHVAQPSENGKDEQHGKADPSPNAA-------VTEKLLVCGLSLLFHLTISNMLPVEYNIDEHFQATASWPTKATYLYVSLLAARPKYYFAWTLADAINNAAGFGFRGYDKNGVARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRVCYERATFS-PTIQTFFLSAIWHGVYPGYYLTFLTGVLMTLAARAVRNNFRHYFLEPPQLKLFYDLITWVATQITISYTVVPFVLLSIKPSFTFYSSWYYCLHVCSILVLLLLP 463
BLAST of MBOAT2 vs. UniProt/SwissProt
Match: sp|Q8BH98|MBOA1_MOUSE (Lysophospholipid acyltransferase 1 OS=Mus musculus OX=10090 GN=Mboat1 PE=1 SV=1) HSP 1 Score: 243.432 bits (620), Expect = 7.155e-71 Identity = 149/453 (32.89%), Postives = 235/453 (51.88%), Query Frame = 2
Query: 80 SGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTI-------FVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGR-KYIRPLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFF 1411
+GS P+S +G+ +DQ NF+ CQL A F F YL+ K SP RH + + G+ +FCFG HL + Y +M S + + + +L++ H++R+Y + YG T D +GPLMI TQK+++LAF +HDG +K E L + HR A+ P+LLE+ SY F ++ GP DY+ FIEGR + E + ++ ++ P K C+ L + F D+F S ++Y Y+++ + KYYFAW++ + +G GF+G + K +D++ N + +KIE TS K ++NWN+ T WL+ Y+R+P Y T+ F+ S++WHG YPGYY F++ T A+RA R Y + + + YDV+T + LA++Y A PF L V+ T ++ +FF
Sbjct: 14 TGSTCLHPLSQLLGIPLDQVNFVACQLFALSAAFWFRIYLHPGKASPEVRHTLATILGIYFVVFCFGWYAVHLFVLVLMCYGVMVTASVSNIHRYSFFVAMGYLTICHISRIYIFHYGILTTDFSGPLMIVTQKITTLAFQVHDGLG-RKAEDLSAEQHRL-AVKAKPSLLEYLSYHLNFMSVIAGPCNNFKDYVAFIEGRHIHM--KLLEVNWTQRGFQSLPEPSPMGAVIQKLCVTLMSLLLFLTLSKSFPVTFLIDDWFVHKANFLSRLWYLYVVMQA----AKPKYYFAWTLADAVHNAAGFGFNGMDTDGKSRWDLLSNLNIWKIETATSFKMYLENWNIQTSTWLKCVCYERVPW-YPTVLTFLLSALWHGVYPGYYFTFLTGVPVTLAARAVRNNYRHHFLSSKARKIAYDVVTWAVTQLAVSYTAAPFVMLAVEPTISLYKSVFF 457
BLAST of MBOAT2 vs. TrEMBL
Match: V9XRU3 (MBOAT2 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 387.497 bits (994), Expect = 2.795e-127 Identity = 186/188 (98.94%), Postives = 186/188 (98.94%), Query Frame = 2
Query: 977 FSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLAPLFSSASGDHSPRVADKISGDDISVH 1540
F GFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNW VGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLAPLFSSASGDHSPRVADKISGDDISVH
Sbjct: 17 FXGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWXVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLAPLFSSASGDHSPRVADKISGDDISVH 204
BLAST of MBOAT2 vs. TrEMBL
Match: A0A267DR73 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016791g1 PE=3 SV=1) HSP 1 Score: 375.941 bits (964), Expect = 1.588e-118 Identity = 205/512 (40.04%), Postives = 294/512 (57.42%), Query Frame = 2
Query: 65 MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTF-RHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRR-------------------------DYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKE-KEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEER-KPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKA-SLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLAPLFSS-ASGDHSPRVAD 1510
MS+FYSGS + P GLTVD+ NF+ CQLVA G F +Y + S R+ + V G L +FCFG QI H+L Q ++M + AR+ F + +LS HL R +YDYGGYT+DITGPLMI TQ+LS+LAFN+HDG +K E R + +H + +P+LL++++Y+ +F GI+VGPFVF D+ ++EG D KE +E++ + S IL K L F L+T+ +Q F ++ D + + K+ +T+ F++RQ+YYFAWS+G +S + SG GF+G +++ K Y V+NFDF +EL TS K IDNWN+ T++WLRECVY+R+P + TLAVF+ S+ WHG+YPGYYL F+S+A FT +R R+++RP + + L YDV+T L + LNY A F L++ W M+FIVHV + L+Y L P SS S +PR A+
Sbjct: 1 MSDFYSGSYLLSPFVPYTGLTVDKLNFIVCQLVAIALGRPFQKYFAAEPGSSNMARYAMSFVVGCLLCLFCFGHQILHVLFQGTVCLLIMLYADRRYARFAACIFAIAYLSACHLMRQFYDYGGYTLDITGPLMISTQRLSTLAFNIHDGIVVKAMEEADRKKKTDDQTEQQESDKSSEKVKPNLTERQMQHFVRDFPSLLQYYTYLVHFQGILVGPFVFYVDFRNYMEGTIPMPSDLTKEQREHYLRGTSRILV-------VAKWTLFFALVTLTMQTVFPLNFLAEDGFQSYSLISKWFYMTLACFVIRQRYYFAWSLGELSYLSSGCGFTGINKDTGKAEYKTVQNFDFNGVELSTSMKMAIDNWNISTVHWLRECVYERVPSKHATLAVFIASATWHGFYPGYYLLFISVAFFTTVARLARRHLRPHFQGSETAKLAYDVVTGLASLTTLNYGAGAFALLDLYKGLRFWWSMYFIVHVIAGLMYLGFVTLKPASSSRRSAPEAPRRAE 505
BLAST of MBOAT2 vs. TrEMBL
Match: A0A1I8HGA1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016791g3 PE=3 SV=1) HSP 1 Score: 374.785 bits (961), Expect = 4.027e-118 Identity = 204/512 (39.84%), Postives = 294/512 (57.42%), Query Frame = 2
Query: 65 MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTF-RHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRR-------------------------DYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKE-KEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEER-KPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKA-SLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLAPLFSS-ASGDHSPRVAD 1510
MS+FYSGS + P GLTVD+ NF+ CQLVA G F +Y + S R+ + V G L +FCFG QI H+L Q ++M + AR+ F + +LS HL R +YDYGGYT+DITGPLMI TQ+LS+LAFN+HDG +K E R + +H + +P+LL++++Y+ +F GI+VGPFVF D+ +++G D KE +E++ + S IL K L F L+T+ +Q F ++ D + + K+ +T+ F++RQ+YYFAWS+G +S + SG GF+G +++ K Y V+NFDF +EL TS K IDNWN+ T++WLRECVY+R+P + TLAVF+ S+ WHG+YPGYYL F+S+A FT +R R+++RP + + L YDV+T L + LNY A F L++ W M+FIVHV + L+Y L P SS S +PR A+
Sbjct: 1 MSDFYSGSYLLSPFVPYTGLTVDKLNFIVCQLVAIALGRPFQKYFAAEPGSSNMARYAVSFVVGCLLCLFCFGHQILHVLFQGTVCLLIMLYADRRYARFAACIFAIAYLSACHLMRQFYDYGGYTLDITGPLMISTQRLSTLAFNIHDGIVVKAMEEADRKKKTDDQTEQQESDKSSEKVKPNLTERQMQHFVRDFPSLLQYYTYLVHFQGILVGPFVFYVDFRNYMDGTIPMPSDLTKEQREHYLRGTSRILV-------VAKWTLFFALVTLTMQTVFPLNFLAEDGFQSYSLISKWFYMTLACFVIRQRYYFAWSLGELSYLSSGCGFTGINKDTGKAEYKTVQNFDFNGVELSTSMKMAIDNWNISTVHWLRECVYERVPSKHATLAVFIASATWHGFYPGYYLLFISVAFFTTVARLARRHLRPHFQGSETAKLAYDVVTGLASLTTLNYGAGAFALLDLYKGLRFWWSMYFIVHVIAGLMYLGFVTLKPASSSRRSAPEAPRRAE 505
BLAST of MBOAT2 vs. TrEMBL
Match: A0A1I8HAD0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016791g2 PE=3 SV=1) HSP 1 Score: 374.015 bits (959), Expect = 8.982e-118 Identity = 204/512 (39.84%), Postives = 294/512 (57.42%), Query Frame = 2
Query: 65 MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTF-RHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRR-------------------------DYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKE-KEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEER-KPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKA-SLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLAPLFSS-ASGDHSPRVAD 1510
MS+FYSGS + P GLTVD+ NF+ CQLVA G F +Y + S R+ + V G L +FCFG QI H+L Q ++M + AR+ F + +LS HL R +YDYGGYT+DITGPLMI TQ+LS+LAFN+HDG +K E R + +H + +P+LL++++Y+ +F GI+VGPFVF D+ +++G D KE +E++ + S IL K L F L+T+ +Q F ++ D + + K+ +T+ F++RQ+YYFAWS+G +S + SG GF+G +++ K Y V+NFDF +EL TS K IDNWN+ T++WLRECVY+R+P + TLAVF+ S+ WHG+YPGYYL F+S+A FT +R R+++RP + + L YDV+T L + LNY A F L++ W M+FIVHV + L+Y L P SS S +PR A+
Sbjct: 1 MSDFYSGSYLLSPFVLYTGLTVDKLNFIVCQLVAIALGRPFQKYFVAEPGSSNMARYAVSFVVGCLLCLFCFGHQILHVLFQGTVCLLIMLYADRRYARFAACIFAIAYLSACHLMRQFYDYGGYTLDITGPLMISTQRLSTLAFNIHDGIVVKAMEEADRKKKTDDQTEQQESDKSSEKVKPNLTERQMQHFVRDFPSLLQYYTYLVHFQGILVGPFVFYVDFRNYMDGTIPMPSDLTKEQREHYLRGTSRILV-------VAKWTLFFALVTLTMQTVFPLNFLAEDGFQSYSLISKWFYMTLACFVIRQRYYFAWSLGELSYLSSGCGFTGINKDTGKAEYKTVQNFDFNGVELSTSMKMAIDNWNISTVHWLRECVYERVPSKHATLAVFIASATWHGFYPGYYLLFISVAFFTTVARLARRHLRPHFQGSETAKLAYDVVTGLASLTTLNYGAGAFALLDLYKGLRFWWSMYFIVHVIAGLMYLGFVTLKPASSSRRSAPEAPRRAE 505
BLAST of MBOAT2 vs. TrEMBL
Match: A0A4S2MC53 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_001090 PE=3 SV=1) HSP 1 Score: 361.303 bits (926), Expect = 3.677e-113 Identity = 184/471 (39.07%), Postives = 279/471 (59.24%), Query Frame = 2
Query: 92 IFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLAPLFSSASGDHSPR 1501
I + ++ ++G+ V Q F+ CQ +A G ++ + RH++EL G+ + +G I HL Q+I Y+M+ M A+Y IL F +++LS H+ +++ + ++DI PLM+QTQKLSSLAFN++DG ++ P+ R+YH+ AL + P LL+ Y+F FH MVGP +F ADY+RFIEGR+ E L + ++ F ++K I E+ VK ++ LLT++ +F ++F +E+++ F KYI L+I F RQK+YFAW++ ++N+ +G GFSGF + P Y N F IELGTSTK+I D+WN T WLREC+YDR+P++Y +VFV S++WHG++PGYYL F+S AL T A R R+Y+RP + A+ YD++T L M LNY I F Y W +M F+ H+ +LL F+ P + A +SP
Sbjct: 3 ISESIAHSLGINVPQVYFVLCQSIAILCGLGLRTWVPPGPNNTKKRHLLELSVGILILYVGYGPCILHLFAQAIPAYLMLVFMPLSVAQYGILLFSMMYLSAVHIYQLFVE-DANSVDIIAPLMVQTQKLSSLAFNINDGVKMSLGLPVLREYHKLSALDRRPRLLQLGGYLFCFHNCMVGPSMFFADYVRFIEGREAERLTDPVLQQRFLEHKDEIRLSRSEVWKQVKLLVIHTLLTVWAFSNFKAEHFINEEFVRKNFFQKYIFLSIACFAFRQKFYFAWTLSCVANLAAGFGFSGFDSQGFPEYGFATNIHFLPIELGTSTKTIFDSWNTATTRWLRECIYDRVPKSYAVWSVFVASAVWHGFHPGYYLAFVSAALITVAGRICRRYLRPYFLGSAATRFSYDIITHLGAMFCLNYLGIAFLLKSTNKVLYFWRQMRFLGHIAPLLLIFILPVICGQPVDAIKLNSPE 472
BLAST of MBOAT2 vs. Ensembl Cavefish
Match: mboat2b (membrane-bound O-acyltransferase domain-containing protein 2-like [Source:NCBI gene;Acc:103039741]) HSP 1 Score: 280.026 bits (715), Expect = 1.605e-85 Identity = 165/466 (35.41%), Postives = 252/466 (54.08%), Query Frame = 2
Query: 80 SGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEG---RQEEVLDNEKEKEYFRKNK-STILAGWPEIGFYVKSCLVF-GLLTIF-VQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTP 1453
+GS + QP+S+ + L +DQ NF+ CQL A F F YL+ K SP RH++ + G+ +FCFG H L QS Y +M + + L L +L + R+Y +DYG Y+ D TGP+M+ TQK++S+AF +HDG K+++ + + A+ + P+LLE+FSY F GI+ GP DY+ FIEG R E+ +N K + R N S + ++G S LVF + +F V+ + D D+ T + ++ K + L + R KYYF W++ N +G GF+G+ + KP +D++ N IEL TS K +DNWN+ T +WL+ Y+R P T A F+ S++WHG YPGYYL F++ + T A+RA R +RP + + YDV+T +A+ Y +PF L V + + +F +H+G VLL P
Sbjct: 10 TGSSLLQPISEIINLPLDQVNFVVCQLCALLTAFWFRLYLHPSKTSPFVRHVVATLLGLYFALFCFGWYALHFLMQSGITYGIMIIAGVEHMHKYCLVVALAYLGFCQITRVYVFDYGMYSADFTGPMMVITQKITSIAFEIHDGMARKEDQLTQS--QKLLAIRRMPSLLEYFSYNCNFMGILAGPTCSYKDYIAFIEGKPFRHRELENNGKLQGKTRLNDPSPNMEVVRKLGTCFLSLLVFLSVCKVFPVERNTDDDFIAT-----TPIYAKVVYLYLSMLTTRPKYYFVWTLADAINNAAGFGFNGYDSDGKPQWDLISNLRIMNIELATSFKVFLDNWNIQTAHWLKRVCYERCPYN-PTAATFLLSAMWHGAYPGYYLTFLTGIVVTLAARAVRHNVRPYFLGSPTHKFVYDVITWASTQIAICYTVVPFVLLSVGPSLKFYRSWYFCLHIGCVLLAIALP 467
BLAST of MBOAT2 vs. Ensembl Cavefish
Match: mboat2a (membrane-bound O-acyltransferase domain-containing protein 2-like [Source:NCBI gene;Acc:103046785]) HSP 1 Score: 262.307 bits (669), Expect = 9.361e-79 Identity = 160/464 (34.48%), Postives = 245/464 (52.80%), Query Frame = 2
Query: 80 SGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEG---RQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFL-VRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTP 1453
+GS + QP+S+ + L +DQ NF+ CQL A F YL+ K SP RH++ + G L +FCFG H L QS Y +M + + L +L + + R+Y +DYG Y+ D TGP+M+ TQK++SLAF +HDG + K E + + A+ K P+LLE+ SY F GI+ GP DY+ FIEG + + + N KE K K + + ++ + +C + L+ + D D Y S FY ++ +S L +R KYYF W++ N +G GF+G++++ P +D++ N IE TS KS +DNWN+ T WL+ Y+R P + T A F+ S++WHG YPGYYL F+S T A+RA R +R + ++ L YDV+T +A+ Y +PF L V + + +F +H+ V+L P
Sbjct: 9 TGSTVLQPISEMIDLPLDQVNFVVCQLFALFTACWFRLYLHPSKTSPFIRHVVATLLGFYLALFCFGWYALHFLVQSGLTYGIMIFTGLEHMHRYSFVVALGYLMLCQITRVYVFDYGMYSADFTGPMMVITQKITSLAFEIHDG--LTKKEEHLKPSQKYLAVRKMPSLLEYLSYNCNFLGILAGPTCSYNDYIAFIEGTGYQHKHLESNGKENG---KCKQSDPSPKRDVILKMSTCALCLLVYLTFYRICPVDRVIQDPYFTSQPFYLRVLYIYLSMLCLRPKYYFVWTLADAINNAAGFGFNGYNKDGTPKWDMISNLRILDIEFATSFKSFLDNWNIQTALWLKRVCYERCPY-HPTAATFLLSAMWHGVYPGYYLTFVSGIGVTLAARAVRHNVRQHFLGSSTHKLIYDVITWASTQIAICYTVVPFVVLAVGPSLRFYWSWYFCLHLACVILILALP 466
BLAST of MBOAT2 vs. Ensembl Cavefish
Match: mboat1 (membrane bound O-acyltransferase domain containing 1 [Source:NCBI gene;Acc:103037745]) HSP 1 Score: 247.669 bits (631), Expect = 2.111e-73 Identity = 147/465 (31.61%), Postives = 237/465 (50.97%), Query Frame = 2
Query: 80 SGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQ-EEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIF--VQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSF-LVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPK 1456
+G +P+S G+ +DQ NF+ CQL F F L+ ++SPT RH + G+S FCFG H+L ++ Y++ S ++ ++ + + +L+V ++R++ + YG + D +GPLM+ TQ+++ LAF++HDG K+++ R+ A+ P+LLE+ SY F I+VGP +Y+ FIEGR + L +E + P K + G + F + F Y E++ F + VS R K+YFAW++ + +G G SG ++ + +D+V N ++IE TS KS IDNWN+ T WL+ YDR+P Y T F S++WHG YPGYY F++ T A+RA R+ R + ++ YDV+T L + Y +PF L V+ T + M+F VH+ S+L + P+
Sbjct: 11 TGCTWLRPLSAITGIPLDQVNFIICQLFGLAIAFWFRLSLSPHRVSPTLRHAVATFLGISFVTFCFGWYSSHVLVLTLVCYLIQCQASVQNVHWYSMLAAVGYLTVCQVSRVFIFSYGVLSTDFSGPLMMVTQRITILAFHVHDGMGRKQDD--LTPEQRRLAVSTKPSLLEYLSYNLNFMSILVGPCSNYKEYVDFIEGRHVQSKLKKLREGSSEPNGWDAVSEPSPVRAVCQKLLVCVGCMVWFFTIAKAFPITYNVDPEFMTQASFLSRLAYAFVSIQAARSKFYFAWTLADAIHNAAGFGISGVDKKGRVSWDLVSNIHIWEIETATSFKSFIDNWNIQTGVWLKSVCYDRVPY-YQTPLTFALSALWHGVYPGYYFTFLTGIPITLAARAVRRNFRQYFLGSPAVKFAYDVVTWAATQLTICYTVMPFLVLAVEPTLEYYRSMYFCVHIISILAIIVLPQ 472
BLAST of MBOAT2 vs. Ensembl Cavefish
Match: mboat1 (membrane bound O-acyltransferase domain containing 1 [Source:NCBI gene;Acc:103037745]) HSP 1 Score: 227.639 bits (579), Expect = 3.337e-66 Identity = 141/465 (30.32%), Postives = 225/465 (48.39%), Query Frame = 2
Query: 80 SGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQ-EEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIF--VQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSF-LVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPK 1456
+G +P+S G+ +DQ NF+ CQL F F L+ ++SPT RH + G+S FCFG H+L ++ Y++ S ++ ++ + + +L+V ++R++ + YG + D +GPLM+ TQ+++ LAF +P+LLE+ SY F I+VGP +Y+ FIEGR + L +E + P K + G + F + F Y E++ F + VS R K+YFAW++ + +G G SG ++ + +D+V N ++IE TS KS IDNWN+ T WL+ YDR+P Y T F S++WHG YPGYY F++ T A+RA R+ R + ++ YDV+T L + Y +PF L V+ T + M+F VH+ S+L + P+
Sbjct: 11 TGCTWLRPLSAITGIPLDQVNFIICQLFGLAIAFWFRLSLSPHRVSPTLRHAVATFLGISFVTFCFGWYSSHVLVLTLVCYLIQCQASVQNVHWYSMLAAVGYLTVCQVSRVFIFSYGVLSTDFSGPLMMVTQRITILAF--------------------------HPSLLEYLSYNLNFMSILVGPCSNYKEYVDFIEGRHVQSKLKKLREGSSEPNGWDAVSEPSPVRAVCQKLLVCVGCMVWFFTIAKAFPITYNVDPEFMTQASFLSRLAYAFVSIQAARSKFYFAWTLADAIHNAAGFGISGVDKKGRVSWDLVSNIHIWEIETATSFKSFIDNWNIQTGVWLKSVCYDRVPY-YQTPLTFALSALWHGVYPGYYFTFLTGIPITLAARAVRRNFRQYFLGSPAVKFAYDVVTWAATQLTICYTVMPFLVLAVEPTLEYYRSMYFCVHIISILAIIVLPQ 448
BLAST of MBOAT2 vs. Ensembl Cavefish
Match: mboat7 (membrane bound O-acyltransferase domain containing 7 [Source:NCBI gene;Acc:103043354]) HSP 1 Score: 113.62 bits (283), Expect = 3.003e-26 Identity = 110/471 (23.35%), Postives = 200/471 (42.46%), Query Frame = 2
Query: 122 LTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPL-----MIQTQKLSSLAFNLHDGQRIKKNE-------PLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGF----------SGFSEERKPIYD--------VVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIP-RAYGTLA--VFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVL 1435
++ D+ ++ + GF+F RYL SP + L+ G+++TI G H L + ++++ S ++ LG+ L+L L + + GP ++ T K+ SLA + KK + P+ K P+L + SY + + GIM GPF ++ ++E Q + + + ++ +K V+G L +FV +F Y +T+E+L+ F++++ + +V F+ R ++Y AW I +GLG G + E P D ++N D + + + + WN+ +WL +Y P RAY A + S+ WHG + GYYL F+++ L A A +R + + +D + L M A +Y + F L+ T WS ++FI+H+ ++L
Sbjct: 1 MSPDELVYLGILAASVPVGFLF-RYL-----SPPVKQGAALLLGLTITIGTCGIHTLHSLCTVLGTWLIIT-FSWRNGPSLSLGWTFLYLLFFRLVTWF------GLPSPGPFANAIQLLLTLKMVSLANEVKSYHSEKKKDVSTFSKSPVVGGLSHK------PSLYDVISYSYCYIGIMTGPFFRYQTFVDWLE--QPDAVSLPGREPCLQR---------------LKMVPVYGALFLFVNSYFPLSYVRTEEFLEHDYFFRFLYMVMVFFVFRMRFYAAWCGAEAGCISAGLGCYPQGALSKPGGGPTVEYSPEPDTAVEYDFKTIQNIDCYNTDFCVKVRHGMRYWNMTVQWWLHHYIYPNAPFRAYALRAGWTMLISAYWHGLHVGYYLSFLTIPLCIAAETAMEASVRVRLGPGGQKV-FDWVHWFLKMRAYDYMCMGFVLLKASDTLNYWSSIYFIIHIIALL 434
BLAST of MBOAT2 vs. Ensembl Sea Lamprey
Match: MBOAT2 (membrane bound O-acyltransferase domain containing 2 [Source:HGNC Symbol;Acc:HGNC:25193]) HSP 1 Score: 167.548 bits (423), Expect = 1.662e-46 Identity = 107/333 (32.13%), Postives = 168/333 (50.45%), Query Frame = 2
Query: 134 QFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIF----VQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFL-VRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRE 1114
Q NF+ CQL+A F F YL+ S RH + + G IFCFG H Q I+ Y ++ + T++ + F + +L+V ++R+Y + G ++ D +GP+M+ TQK++SLAF +HDG IK+++ L + R+ A + PTLL+F S+ F I+ GP DY FIEG + E+ R+N P V S L+ LL++ + F + ++ S + + VS R KYYFAW++ N +G GF+G+ + + +D+V N + + IE TS K IDNWN+ T WL+
Sbjct: 1 QVNFVMCQLLALAMAFFFRLYLHPRAASERVRHGLATLLGSYFAIFCFGWYATHFFVQVIASYAIIVLVDTRNVHKYTFIFAMGYLTVCQISRVYIFTSGVFSTDFSGPMMVMTQKITSLAFEIHDGM-IKEDQDLTVE-QRRLASRRLPTLLQFLSFNLNFLSILAGPSSSFKDYTAFIEGSHYK--GWERSGACGRENGPL---HEPSPAAAVTSKLLMVLLSLVFYLTLSKAFPVSLNADEAFIASAPLHARLCYLYVSCQGGRPKYYFAWTLADAINNAAGFGFNGYDKNGRSKWDLVSNLNIWNIETATSFKMFIDNWNIQTALWLKR 326
BLAST of MBOAT2 vs. Ensembl Sea Lamprey
Match: MBOAT2 (membrane bound O-acyltransferase domain containing 2 [Source:HGNC Symbol;Acc:HGNC:25193]) HSP 1 Score: 119.398 bits (298), Expect = 2.359e-30 Identity = 61/163 (37.42%), Postives = 94/163 (57.67%), Query Frame = 2
Query: 911 VRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQ--LEVQLTFY 1390
R KYYFAWSI N +G GF+G+ + + +D++ N + + IE TS K +DNWN+ T+ WL+ YDR+ + Y T+A FV S+ WHG YPGYY F++ + + ++RA R R + ++ + YDV+T L++ Y PF ++ L FY
Sbjct: 53 ARPKYYFAWSIADAINNAAGFGFNGYHKNGEARWDLISNLNIWNIETATSFKMFLDNWNMMTVRWLKYVCYDRV-KTYPTVATFVLSAAWHGVYPGYYFTFLTGIIMSLSARAMRNNFRHYFLGSKTVKVAYDVVTWAATQLSICYTVAPFVMYGMKPALDFY 214
BLAST of MBOAT2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006365.1 (pep scaffold:Pmarinus_7.0:GL478876:4587:32960:-1 gene:ENSPMAG00000005745.1 transcript:ENSPMAT00000006365.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 105.145 bits (261), Expect = 3.281e-26 Identity = 67/186 (36.02%), Postives = 101/186 (54.30%), Query Frame = 2
Query: 134 QFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQ-SISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNE--PLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQ 682
Q NF+ C L+A F YL+ SP RH + G+ L IFC+G H Q +IS Y+++ A + K + + LS+ + +DYG Y+ D TGP+M+ QK+++LA+++HDG K + P +R Y +H + PTLL+++SY F F I+ GP DYL F+EGR
Sbjct: 1 QVNFVACLLLALLAACWFRLYLHPSHASPAVRHSVATFLGIYLAIFCYGWYTLHFFAQVTISYYVLLFA-NGKHVHIYSFIIAMGCLSLCQIKFFIFDYGIYSTDFTGPMMVMAQKITTLAYSVHDGIGRKDEDLSPEQRRYAVRHRVR--PTLLQYYSYNFNFLSILTGPCCAYKDYLAFMEGRH 183
BLAST of MBOAT2 vs. Ensembl Yeast
Match: ALE1 (Broad-specificity lysophospholipid acyltransferase; part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids [Source:SGD;Acc:S000005701]) HSP 1 Score: 151.754 bits (382), Expect = 4.862e-39 Identity = 109/378 (28.84%), Postives = 187/378 (49.47%), Query Frame = 2
Query: 374 FVLLFLSVAHL-ARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRK-HALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIG----FYVKSCLVFGLLTIFVQPHFDPDY-FKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGF-SEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRI------PRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP--LVHKASLNLPYDVLTSLLGM----LALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFL 1447
FV+ L++ H+ A+ + T+DIT M+ KL+S A++ +DG +++ H+K A+ +P LL+F +Y F++ ++ GP AD+ ++ E D + K+ R++ P+ G + V L + +L+ HF Y D + ++ L ++ F+ R KYY AW+I S I GLG++G+ S+ +K +D V+N D + +E +T+ +++ WN+ T WL+ VY R+ P TL F+ S+ WHG PGYYL F + AL+ + R+ RP L LP + L+G+ LA Y PF L+++ + +W ++F VH+ +FL
Sbjct: 101 FVMGHLAINHIHAQFLNEQTQTTVDITSSQMVLAMKLTSFAWSYYDGSCTSESDFKDLTEHQKSRAVRGHPPLLKFLAYAFFYSTLLTGPSFDYADFDSWLNC--EMFRDLPESKKPMRRHHPGERRQIPKNGKLALWKVVQGLAWMILSTLGMKHFPVKYVLDKDGFPTRSFIFRIHYLFLLGFIHRFKYYAAWTISEGSCILCGLGYNGYDSKTQKIRWDRVRNIDIWTVETAQNTREMLEAWNMNTNKWLKYSVYLRVTKKGKKPGFRSTLFTFLTSAFWHGTRPGYYLTFATGALYQTCGKIYRRNFRPIFLREDGVTPLPSKKIYDLVGIYAIKLAFGYMVQPFIILDLKPSLMVWGSVYFYVHIIVAFSFFL 476
BLAST of MBOAT2 vs. Ensembl Nematostella
Match: EDO40788 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6B5]) HSP 1 Score: 195.282 bits (495), Expect = 1.665e-55 Identity = 130/415 (31.33%), Postives = 212/415 (51.08%), Query Frame = 2
Query: 185 FSRYLNTDKISPTFRHIIELVCGVSLTIF--CFGSQIWHLLGQSISVYIMMNAMSTKS--ARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEY----------FRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYL--QSGMFYK--YIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLNLP-YDVLTSLLGMLALNYCAIPFTQLE 1372
+ + L+ ++S R + L+ G+ L+ C+ + H L VY++ + + +KS ++ + LS AHL R YG Y +D TGPLMI TQK+ +AF++HDG + ++E +K L K P+ LE+F Y+F++ I++GP +Y FI G + N K F K+T+ + S ++ ++ + P F P D Y + Y+ Y L+IV+ R +YYFA+ G N GLGF G+ EE P ++ + N + K+EL + K ++DNWN+ T WLR VYDR+ ++ TLA F+ S+ WHG+Y GYYL F++ + EA+R R+ +R + + YDV+T ++ M+ + Y +PF L+
Sbjct: 2 YRKVLHPSRVSSKTRQTVGLLAGLFLSWLKQCWSWFLLHFL--MFCVYVLNSGVFSKSYAKNQYVSVVAMGILSAAHLYRQITVYGSYVLDYTGPLMIITQKIIYVAFSVHDG--MGRDESNLNAEQKKEKLKKVPSPLEYFGYVFHYSNILIGPVGTYHEYTDFITG----TVFNTKVGPVGTCHTVPWAAFNTKKATVEK--------LSSAILCVVVFVVASPRF-PVASNADPYFIDNTPFIYRLIYAWLSIVA--TRMRYYFAFKAGEAINNICGLGFGGYDEEGNPSWNKMSNANITKLELAQNMKMLLDNWNISTAVWLRRIVYDRVT-SHRTLATFLLSAFWHGFYGGYYLTFITCQILVEAARVARRTLREHFQGSKMTSRFYDVITCVVTMVFVTYATVPFVLLD 396
BLAST of MBOAT2 vs. Ensembl Nematostella
Match: EDO40083 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8D7]) HSP 1 Score: 126.331 bits (316), Expect = 5.828e-31 Identity = 105/445 (23.60%), Postives = 184/445 (41.35%), Query Frame = 2
Query: 215 SPTFRHIIELVCGVSLTIFCFGSQ-IWHLLGQSISVYIMMNAMSTKSARY----FILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFL--VRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPI----------------------------YDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIP---RAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVL 1435
SP + + + GV L++ G IWH L I Y+++ ++ + ++ F+ G++ F + Y+ + M ++ T +L +L F + D + I +N K A P+ EF SY + + G++ GP+ Y F+E ++N I + WP + +K ++G L + + +F + ++E+ + Y +L +V R ++Y W + S I GLG F + KP + V N D FK+E S +SI+ +WN+ +W+ + V+ ++P R + S+ WHG PGYYL F+S+ L A K I+P + + + YD + A+ Y F L+ IW M+FI H+ VL
Sbjct: 27 SPARKQFLSTLAGVGLSLALLGPWGIWHSLTAVIGAYLIVASLGPRRCQWVAFLFVFGYLFFFRTCT-----YFGFKKPPMQSNAVQLLVTLRLCTLPFEIFDPE-ISRN---------KAAATSKPSFYEFLSYSYCYCGLLTGPYYRYKTYKDFLE----------------QENPEKIASLWPALR-RLKFAPLYGGLYLLMNTYFPTKHLMSEEFFKHPWGIPYQLLYLVPAFNGFRWRFYIGWLLAESSCIMLGLGAYPFETDPKPALGPSKPMPAENGHVSEVADTKENGDTHSFMTVYNIDIFKVEFAPSMRSIMKDWNMSVQWWIAQYVHRKLPFSNRNIRMSVTLLVSAFWHGVAPGYYLTFLSVPLVVMAEVRMEKAIKPWLSQ-RMCYYYDWCSWFCLYRAMEYLGCGFILLQFAPCIAIWKNMYFIGHIIMVL 438
BLAST of MBOAT2 vs. Ensembl Nematostella
Match: EDO46261 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQA5]) HSP 1 Score: 106.686 bits (265), Expect = 2.097e-24 Identity = 107/355 (30.14%), Postives = 169/355 (47.61%), Query Frame = 2
Query: 458 LMIQTQKLSSLAFNLHDG-QRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEG---RQEEVLDNEKEKEYFRKNKSTILA---GWPEIGFYVKSCL-VFGLLTIF---VQPH--FDPDYFKTDEYL-QSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLNLP-YDVLTSL----LGMLALNYCAIPFTQLEVQLTF-YIWSKMFF--IVHVGSVLLYFLTPK 1456
LMI QKL+ +AF+LHDG R KN +K + P+ LE+ +YMF+++ ++VGP L +Y FI G RQ + F + +L G P F VK + G+ IF + P ++ + E++ S + Y+ + F++R KYYF ++IG + I SG GF+G+ +P +D ++ + K+E + ID L VYDR+P L F+ S IWHG+YPGY+ L+ +A R++ + LN+P Y L +L ++ +YCA PF L Y S +FF I+ + ++LL +T K
Sbjct: 70 LMIMFQKLTYVAFSLHDGCGREDKNLT---QSQKKEKITDIPSFLEYLAYMFHYNMVLVGPTCTLREYRDFITGQSLRQPITGCETAAGKRFLVAMAIVLGFIVGSPS--FPVKRNVGESGMKRIFSLNIMPGGLYEKGGGQDAEFIASSSLLYRIFYAWLSCFILRLKYYFVFTIGEVGVIISGQGFNGYDVRGRPRHDAIRYVNILKVETDGQARGWID---------LACVVYDRVPLHKQAL-TFMTSLIWHGFYPGYFAAAAFAYLYLVTGKAVRRHFGHVYE--GLNVPAYVTLLALNIAVCCLMNTHYCA-PFVLLRFDFCCRYYRSVLFFGEIMCLSTLLLVSITGK 406
BLAST of MBOAT2 vs. Ensembl Nematostella
Match: EDO40458 (Porcupine-like protein; Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7A0]) HSP 1 Score: 48.521 bits (114), Expect = 7.299e-6 Identity = 59/254 (23.23%), Postives = 100/254 (39.37%), Query Frame = 2
Query: 446 ITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPH-FDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFK---IELGTSTKSIIDNWNVGTIYWLRECVY---DRIPRAYGTLAVFVCSSIWHG 1186
+ G MI K+ S+AF+ G L + P LLE+F Y I+ GPF+ DY + + G++ + F +S +L C + L ++ V P+ F D K Q+ M +++ +YF + ++I G+G ++ K + +FD + +EL S ++ +WNV +L+ V+ R R L F CSS+ HG
Sbjct: 133 VRGTQMILVMKMISIAFDYDQG-----------------ILAQLPNLLEYFGYSISASSIIFGPFMSYDDYSKILTGKRLSL------SWIFGVTRSFLL------------CFICLLHSVCVSPYIFAEDDNKWLVAYQTAMSFRF------------SHYFVSYLSECTSIACGIGTETIFKDHKQTH---WHFDVSRPQHVELPRSLVEVVIHWNVPNHVFLKNYVFKKTQRFGRFIAILLTFGCSSLLHG 336
BLAST of MBOAT2 vs. Ensembl Medaka
Match: mboat2a (membrane bound O-acyltransferase domain containing 2 [Source:NCBI gene;Acc:101170063]) HSP 1 Score: 257.684 bits (657), Expect = 5.601e-77 Identity = 157/454 (34.58%), Postives = 240/454 (52.86%), Query Frame = 2
Query: 80 SGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEG-----RQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPH-FDPDYFKTDEYL--QSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFF 1411
+GS + QP+S+ + L +DQ NF+ CQL A F YL+ K SP RH++ + G L +FCFG H L QS Y +M + + L +L V + R+Y +DYG Y+ D TGP+M+ TQK++S+AF +HDG + K E + A+ + P+LLE+ SY F GI+ GP DY+ FIEG R +E N KE F++++ + P+ K C L +++ + P + D+Y + ++ + I L + +R KYYF W++ N +G GF+G++++ P +D++ N IE TS K +DNWN+ T WL+ Y+R P T A F+ S++WHG YPGYYL F++ T A+RA R IRP + S L YDV+T +A++Y +PF L V + +S +F
Sbjct: 10 TGSTLLQPLSEIINLPLDQVNFVACQLFALLMAMWFRIYLHPSKTSPFIRHVVATLLGFYLALFCFGWYSLHFLVQSGLSYGVMIFAGLEHMHKYCFIVTLGYLIVCQITRVYVFDYGMYSADFTGPMMVITQKITSMAFEIHDG--LAKKESQLTPNQKYLAISRMPSLLEYLSYNCNFMGILAGPTCSYRDYMAFIEGTCYQARHQENA-NGKENGKFKQSEPS-----PKNDVISKLCTCAISLAVYLSLYKLLPVEYSIDDYFVKSTPLYLQVIYLYLAMLALRPKYYFVWTLADAINNAAGFGFNGYNKDGSPRWDLISNLRILDIEFATSFKLFLDNWNIQTALWLKRVCYERCP-VNPTAATFLLSAMWHGVYPGYYLTFVTGIGMTMAARAVRHNIRPYFLVSDSHKLVYDVITWACTQVAISYTVVPFVLLAVGSSLKFYSSWYF 454
BLAST of MBOAT2 vs. Ensembl Medaka
Match: mboat1 (membrane bound O-acyltransferase domain containing 1 [Source:NCBI gene;Acc:101157881]) HSP 1 Score: 257.299 bits (656), Expect = 9.627e-77 Identity = 160/501 (31.94%), Postives = 255/501 (50.90%), Query Frame = 2
Query: 50 QVSDIMSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKN---KSTILAGWPEIGFYVKSC----LVFGLLT--IFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIR-PLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLAPLFSSASGDHSPRVADKIS 1519
+ SD + +GSK+ PVS+ +G +DQ NF+ CQL F F YL P RH + + GV+ +FCFG H+L +++Y+++ + + + +L+V ++R++ ++YG + D +GPLM+ TQ++++L F LHDG KK+E L + + A+ + P+L+E+ SY F I+VGP DY+ FIEGR + N K+ L+ + + C L F ++T + ++ + +PD+ +L Y +I + R K+YFAW++ N +G GF G E KP +D+V N + IE TS K+ IDNWN+ T WL+ YDR P+ L F+ S++WHG YPGYY F++ T A+RA RK +R ++ L L YD++T LA+ Y +PF L + T + M+F VH+ S+L + G H P+ A +S
Sbjct: 3 ERSDAIMFKTTGSKLLLPVSEYLGFPLDQVNFIACQLFGLAVAFWFRLYLKPSHFKPLVRHTVATLLGVTFLVFCFGWYATHILAIVVAIYVIILKADINHVHRYSMVTAMGYLTVCQVSRVFIFNYGVLSTDFSGPLMMVTQRITTLVFQLHDGM-CKKSEQLTSE-QKLMAIRERPSLIEYLSYNLNFLSILVGPCSNYRDYIDFIEGRHISRRLKQHSGSCNGHNGYDKTPDLSPLNAVCRKLLVCCGCMLTFLIVTRSLPIKYNVEPDFVNNAHFLTR---LTYAFFSIQA--ARPKFYFAWTLADAVNNAAGYGFLGIDENGKPSWDLVSNLNIVGIETATSFKTFIDNWNIRTGVWLKTVCYDRAPKHRLALT-FILSALWHGVYPGYYFTFLTAIPITMAARAIRKRVRHHFLNSKGLKLGYDIVTWAATQLAICYTVMPFLLLSIDQTLTYYRSMYFHVHILSILAVI----------TLHGKHKPKDASSVS 485
BLAST of MBOAT2 vs. Ensembl Medaka
Match: mboat2a (membrane bound O-acyltransferase domain containing 2 [Source:NCBI gene;Acc:101170063]) HSP 1 Score: 231.106 bits (588), Expect = 3.127e-67 Identity = 148/434 (34.10%), Postives = 223/434 (51.38%), Query Frame = 2
Query: 140 NFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMY-YDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEG-----RQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPH-FDPDYFKTDEYL--QSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP-LVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFF 1411
NF+ CQL A F YL+ K SP RH++ + G L +FCFG H L QS Y +M + + L +L V + R+Y +DYG Y+ D TGP+M+ TQK++S+AF +HDG K N ++ P+LLE+ SY F GI+ GP DY+ FIEG R +E N KE F++++ + P+ K C L +++ + P + D+Y + ++ + I L + +R KYYF W++ N +G GF+G++++ P +D++ N IE TS K +DNWN+ T WL+ Y+R P T A F+ S++WHG YPGYYL F++ T A+RA R IRP + S L YDV+T +A++Y +PF L V + +S +F
Sbjct: 27 NFVACQLFALLMAMWFRIYLHPSKTSPFIRHVVATLLGFYLALFCFGWYSLHFLVQSGLSYGVMIFAGLEHMHKYCFIVTLGYLIVCQITRVYVFDYGMYSADFTGPMMVITQKITSMAFEIHDG---KTN-------------VRMPSLLEYLSYNCNFMGILAGPTCSYRDYMAFIEGTCYQARHQEN-ANGKENGKFKQSEPS-----PKNDVISKLCTCAISLAVYLSLYKLLPVEYSIDDYFVKSTPLYLQVIYLYLAMLALRPKYYFVWTLADAINNAAGFGFNGYNKDGSPRWDLISNLRILDIEFATSFKLFLDNWNIQTALWLKRVCYERCP-VNPTAATFLLSAMWHGVYPGYYLTFVTGIGMTMAARAVRHNIRPYFLVSDSHKLVYDVITWACTQVAISYTVVPFVLLAVGSSLKFYSSWYF 437
BLAST of MBOAT2 vs. Ensembl Medaka
Match: mboat7 (membrane bound O-acyltransferase domain containing 7 [Source:NCBI gene;Acc:101161994]) HSP 1 Score: 108.997 bits (271), Expect = 1.160e-24 Identity = 96/404 (23.76%), Postives = 167/404 (41.34%), Query Frame = 2
Query: 293 HLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKH---ALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKP------------------IYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIP-RAYGTLA--VFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSV 1432
H L I +I++ + S + A L + L+L L ++D T +I T K+ SLA +H KK + + + L + P+L + SY + + GIM GPF ++ ++ R+ L GW +K V+ L + V F Y +T+E+L+ +F++ + V F+ R ++Y AW I +GLG KP + ++N D + + + + WN+ +WL +Y P ++Y A + S+ WHG +PGYYL F+++ L A A +R + N+ +D + L M A +Y + F L+ T W ++FI+H+ +V
Sbjct: 51 HSLVTVIGTWIIIKS-SGRLAPALSLSWTFLYLLFFRLV-TWFDLPKPTPFANAIQLILTLKMVSLANEVHSFHTEKKKD--VSSFSKPSVIGGLSQEPSLYDVLSYSYCYMGIMTGPFFRFQTFM-----------------DWLRQPNPQALPGWEPCVQRLKLVPVYSALFLGVNHFFPLAYVRTEEFLEQNIFFRMFYMVAVFFVFRMRFYAAWCGAEAGCITAGLGCYAEKALSKPGGGPTVNYSPDPNTKEKYDFKTIQNIDCYNTDFCVKVRHGMRYWNMTVQWWLHHYIYPNAPFKSYTLRAGWTMLISAYWHGLHPGYYLSFLTIPLCIAAESAMEASVRSKLGPGGQNI-FDWVHWFLKMRAYDYMCMGFVLLKAPDTINYWMSIYFIIHIIAV 432
BLAST of MBOAT2 vs. Ensembl Medaka
Match: lpcat3 (lysophosphatidylcholine acyltransferase 3 [Source:NCBI gene;Acc:101154975]) HSP 1 Score: 104.375 bits (259), Expect = 3.419e-23 Identity = 92/403 (22.83%), Postives = 163/403 (40.45%), Query Frame = 2
Query: 83 GSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMS---TKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKH-ALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIP----RAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIR 1267
+ + Q +S+++G + L+ ++ R+L T H+ L G++L F FG+Q++H + ++M+ M T F V L + A YD + T P + T KL LAF+ +DG + EP + +K AL P+LLE + +++ G +VGP L Y + + G + + P + + L + TIF P++ Y+ TDE+ +Y+ + + + ++ KY W I I +GLG++G + + +D N + E I ++N+ T W V+ R+ + + V +IWHG + GY LCF R + +R
Sbjct: 2 AAPLMQKLSESLGSPEPAVRLILSILIGYPCAVVYRRFLFHQP--ATVLHLFHLFSGLALAAFNFGAQLYHSAVCILVQFLMLRLMGRTVTTVLSSFTFQMVYLLAGYYYTATEQYD-----IKWTMPHCVLTLKLVGLAFDYYDGGK----EPSKLSLEQKSSALPSVPSLLEVAGFSYFYGGFLVGPQFTLRSYQKLVAGELTDCPGQPPNSVF------------PAMKRFGLGFLFLAIYTIFA-PYYQDSYYLTDEFEAQPFWYRCVFILLWGKVMLYKYVSCWVIAEGVCILTGLGYNGMVDGKHQ-WDACANMKVWLFETTPLFGGTIASFNINTNAWAARHVFKRLKFLGNKTLSHVTTLVFLTIWHGLHSGYILCFSMEFFIITVERQAQALVR 379
BLAST of MBOAT2 vs. Planmine SMEST
Match: SMESG000066852.1 (SMESG000066852.1) HSP 1 Score: 1030.78 bits (2664), Expect = 0.000e+0 Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 2
Query: 65 MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLAPLFSSASGDHSPRVADKISGDDISVHXXXXXXXXXXXXXXXXXXTVKEDKKID 1621
MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLAPLFSSASGDHSPRVADKISGDDISVHNSIRNENISNNTTNNAENTVKEDKKID
Sbjct: 1 MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFLTPKLAPLFSSASGDHSPRVADKISGDDISVHNSIRNENISNNTTNNAENTVKEDKKID 519
BLAST of MBOAT2 vs. Planmine SMEST
Match: SMESG000074104.1 (SMESG000074104.1) HSP 1 Score: 612.453 bits (1578), Expect = 0.000e+0 Identity = 276/461 (59.87%), Postives = 360/461 (78.09%), Query Frame = 2
Query: 65 MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLLYFL 1447
MSNF+SGSKI P S+AVGL +DQFNF+FCQ+ A G G++FSRYL+ KI+P ++HI E++ G+ LT+FCFG QIW+LL QSISVY+MMN +S + ARY IL FVL FLSVAH+ RMYYDYG YT+DITGPLMIQTQKLSSLAFNL DGQR+K E LR+DYH+ HA+ YPT+LEFFSY FYFHG+MVGPFVFL DYLRFIEGRQ+E+L NE +K +F++NK IL+ WPEI F++ S + + LL F++P+F P++ +DE+ Q+G F +Y+++TI FLVRQKYY AW++GS++N+F+GLGFSGF E+++P Y +VKNF F + E GTS K++ID+WNVGT WLRECVYDR+P + GT+ VF CS+IWHGYYPGYYL F S+AL T A+R GR+ R H A L LPYD+ T+ + M LN+ IPF L+++ F +W ++ + VG +L F+
Sbjct: 1 MSNFHSGSKILLPFSEAVGLKIDQFNFLFCQISACGIGYLFSRYLSRAKINPNYKHISEIILGILLTLFCFGLQIWYLLAQSISVYVMMNVLSVRKARYCILIFVLFFLSVAHIGRMYYDYGSYTLDITGPLMIQTQKLSSLAFNLMDGQRVKNKEKLRKDYHQVHAINHYPTVLEFFSYTFYFHGLMVGPFVFLGDYLRFIEGRQDELLINEDDKNHFKRNKFQILSAWPEISFHLFSLVCYSLLFFFIEPYFKPEFLLSDEFYQNGFFKRYLLMTITMFLVRQKYYIAWTMGSITNLFAGLGFSGFDEKQQPKYHIVKNFHFSRTEFGTSAKTMIDHWNVGTAIWLRECVYDRVPHSIGTITVFTCSAIWHGYYPGYYLAFASVALMTNAARCGRRLFRTYFHNAGLKLPYDITTATMSMFCLNFSIIPFALLDIRKGFMVWKSLYILGPVGCTILLFI 461
BLAST of MBOAT2 vs. Planmine SMEST
Match: SMESG000075570.1 (SMESG000075570.1) HSP 1 Score: 412.535 bits (1059), Expect = 1.486e-136 Identity = 218/474 (45.99%), Postives = 300/474 (63.29%), Query Frame = 2
Query: 65 MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFSE-ERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRP--LVH--KASLNLPYDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGS----VLLYFLTPKL 1459
MS FY+G + F+P+S G+ VDQFNF+FC A G F + IS RH + G +L +FCFG QI HL I Y +M + + +RY +L F + +L+V HLAR Y+DYGGYT+DITGPLM+ Q+LS LAFNL+DG +IKK L D KHA+L++PT L+FFSY+ F IM+GPF+F +DYLRFIEGR+ E + KEKEYF ++++IL+ Y L+ G L +FV P F P ++ T E+ Q K I + IVS L+RQ+YYFAWS+ S + SGLGFSGF++ KP Y ++ NFD IE+ + K+IID WN WLREC+Y+R P+ + T AVF+CS+IWHG+YPGYYL F++ +F E SR R+YIRP LV+ + YDVLT+L+ M A+NY ++PF L + + W + +F H+ +L+ FL PK+
Sbjct: 1 MSKFYAGVRFFEPLSSKFGIHVDQFNFVFCMFAACLMGRQFQTKYSIKNISAEQRHRFAFLGGFTLALFCFGHQIMHLFIPPIISYCIMYFLPYQHSRYIVLLFAMSYLAVTHLARQYWDYGGYTLDITGPLMVLVQRLSILAFNLYDGDQIKKKRKLDFDDAIKHAVLEHPTPLQFFSYVINFQTIMIGPFIFYSDYLRFIEGREIEYAETSKEKEYFVVHRTSILSPEDVTKKYFIIMLICGGLHLFVFPFFQPTFYLTPEFGQYSFINKVIFMLIVSPLLRQQYYFAWSLTSACCLCSGLGFSGFNKISGKPEYKLIDNFDMRNIEMAKNMKAIIDGWNKPANQWLRECIYNRAPKKFATPAVFICSAIWHGFYPGYYLFFVTAGIFLEISRMARRYIRPYFLVNFGDGQTSKFYDVLTTLISMFAINYMSVPFVLLGLSKSIQFWRQFYFCGHLAVPALLILIRFL-PKI 473
BLAST of MBOAT2 vs. Planmine SMEST
Match: SMESG000051516.1 (SMESG000051516.1) HSP 1 Score: 349.362 bits (895), Expect = 2.999e-112 Identity = 191/457 (41.79%), Postives = 273/457 (59.74%), Query Frame = 2
Query: 65 MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFVLLFLSVAHLARMYYDYGGYTMDITGPLMIQTQKLSSLAFNLHDGQRIKKNEPLRRDYHRKHALLKYPTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQEEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCL--VFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVS-FLVRQKYYFAWSIGSMSNIFSGLGFSGFSEERKPIYDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIPRAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYIRPLVHKASLNLP-YDVLTSLLGMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHV 1423
MS FY GS I P+S+ +G+ VDQ NF+ C L++ G G I K+S + R IIE G+ + +FCFG Q+ +LL QS Y+++ + ++ + + + + + F+++ HL RM+YDYGGYT+DI+GP+MIQTQKL+SLAFNL DG RI + L++ H HA+ K P ++FF Y FYFHG++ GPFVF +Y +I G ++ + K K S+ L CL + GLLT +V P F + + +Y + M + IM T++S FLVRQKYYFAWS+ + +GLG+ G + + + VKN+DF IE G+S K ++D WN+ T WLRE +Y+R P T+ VF S+ WHG+YPGYYL F++ +LFT +R RK+IRPL + + L Y VLT + LALNY PF LE Q W +F HV
Sbjct: 1 MSQFYGGSTILAPLSNVMGIPVDQINFIVCSLLSIGLGQIMRHKFPPSKLSGSTRGIIEAFFGILIVLFCFGYQVKYLLLQSTVSYVILLFLPSQISAHLVTYWCMAFMAIVHLYRMHYDYGGYTLDISGPVMIQTQKLTSLAFNLLDGHRINQGIKLQQRNHESHAVKKLPDAIKFFGYTFYFHGVLAGPFVFYNEYKEYITGYDDKHIPCSK------KQLSSAL------------CLSVIHGLLTAYVAPQFPYIFVCSTDYQRMHM-TRRIMFTMISFFLVRQKYYFAWSMVEAGALSTGLGYCGKDKNGENNWSKVKNYDFMAIESGSSLKVLVDGWNISTTRWLRELIYERAPLFCRTILVFTVSAFWHGFYPGYYLMFLTFSLFTFCARTVRKHIRPLFIVSPITLQLYHVLTKIFTNLALNYGQGPFYLLEFQSGLLFWKPYYFFGHV 438
BLAST of MBOAT2 vs. Planmine SMEST
Match: SMESG000059008.1 (SMESG000059008.1) HSP 1 Score: 106.686 bits (265), Expect = 4.759e-24 Identity = 116/492 (23.58%), Postives = 207/492 (42.07%), Query Frame = 2
Query: 122 LTVDQFNFMFCQLVASGFGFIFSRYLNTDKISPTFRHIIELVCGVSLTIFCFGSQIWHLLGQSISVYIMMNAMSTKSARYFILGFV-----LLFLSVAHLARMYYDYG-GYTMDITGPLMIQTQKLSSLAFNLHDG-QRIKKNEPLRRDYHRK-------HALLKY----PTLLEFFSYMFYFHGIMVGPFVFLADYLRFIEGRQ-EEVLDNEKEKEYFRKNKSTILAGWPEIGFYVKSCLVFGLLTIFVQPHFDPDYFKTDEYLQSGMFYKYIMLTIVSFLVRQKYYFAWSIGSMSNIFSGLGFSGFS------------EERKPI-------YDVVKNFDFFKIELGTSTKSIIDNWNVGTIYWLRECVYDRIP-RAYGTLAVFVCSSIWHGYYPGYYLCFMSLALFTEASRAGRKYI---RPLVHKASLNLPYDVLTSLL-----------GMLALNYCAIPFTQLEVQLTFYIWSKMFFIVHVGSVLL 1438
+T D F + F +++ FI ++KI +F+ + G+ I G+ +WH L ++ I + + K + IL F+ L+F HL + +D Y+ I ++ T KL AF + D +R +K + L + +KY +++ F+Y + F G++ GP+ Y +++ Q + + E++ + +K+ G+ +F + +Y K+DE+ FY+++ ++++ F+ R + YFAW I + +G+G S + +PI YD +N + E S + + +WN+ YWL VY R P +AY T + S+ WHG +PGYYL FM++ L A A K + P N + S+L M +YC + F LE T W ++F VH+ ++L
Sbjct: 1 MTWDDFIYAFMLILSIFCAFI------SNKIQLSFKKYFMSLFGLLFVICVSGTAVWHSLFVTL---ISIGIIGFKLKHFHILVFIFCFGYLIFFRTCHL--IGFDRPPSYSNAIQ---LVLTLKLVGAAFEIKDSLERERKLKILLNTIQMEDNEIEDLKLEIKYFNVKSDMIDLFTYSYCFIGLLTGPYF---KYRVYLDCSQFSSTVYVNRYSEFWNR---------------LKNAAFIGVAYLFASHFYFIEYAKSDEFYNRSFFYRFVFMSVMFFVFRMRMYFAWLISECVCMCAGMGAYPASSVPVPGHGPTNLKGMEPIGDSIAVSYDTTRNLNISGCEWAPSVRDGMRSWNMSVQYWLATFVYRRFPIKAYRTFVTMLVSAFWHGVHPGYYLSFMTIPLSLLAESALEKVVIGAAPTSSARIRNANGEWQPSMLIEKSFFIRWFYKMRVFDYCCMGFLLLEAGSTLMYWWSIWFSVHLLMIVL 460 The following BLAST results are available for this feature:
BLAST of MBOAT2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of MBOAT2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of MBOAT2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of MBOAT2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of MBOAT2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of MBOAT2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of MBOAT2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of MBOAT2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of MBOAT2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of MBOAT2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 3
BLAST of MBOAT2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of MBOAT2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 4
BLAST of MBOAT2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of MBOAT2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30014008 ID=SMED30014008|Name=MBOAT2|organism=Schmidtea mediterranea sexual|type=transcript|length=2265bpback to top protein sequence of SMED30014008-orf-1 >SMED30014008-orf-1 ID=SMED30014008-orf-1|Name=SMED30014008-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=520bp MSNFYSGSKIFQPVSDAVGLTVDQFNFMFCQLVASGFGFIFSRYLNTDKIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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