MFS domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30014006 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12
Homology
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A0H3YF06 (Slc16a-5 (Fragment) OS=Schmidtea mediterranea OX=79327 GN=slc16a-5 PE=2 SV=1) HSP 1 Score: 76.6406 bits (187), Expect = 1.269e-12 Identity = 43/75 (57.33%), Postives = 53/75 (70.67%), Query Frame = 1 Query: 46 KGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYR-T*QYVGNQR*HGMGSFSLAVFSQAFTMSLGGLLEKKXG 267 KG C+ GGFLI FSLG+IYTFGN+SPYIASY++ R Q V N + +F+LA SQ TMS GG+L+KK G Sbjct: 11 KGYLCILGGFLIHFSLGSIYTFGNISPYIASYMRSRPVTQSVKNSDMVWIFAFALA--SQGLTMSFGGVLDKKVG 83
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1I8HYG0 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 72.0182 bits (175), Expect = 6.202e-11 Identity = 36/75 (48.00%), Postives = 51/75 (68.00%), Query Frame = 1 Query: 46 KGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRT-*QYVGNQR*HGMGSFSLAVFSQAFTMSLGGLLEKKXG 267 KGI V GGFLI F+LGT+YTFGNMSPY+ SY++ + +++G F L++F Q +M+ GGL+EK+ G Sbjct: 6 KGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYP--DATWIFGLSIFGQGASMAFGGLIEKRIG 78
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A267H5U2 (MFS domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000477g2 PE=4 SV=1) HSP 1 Score: 72.0182 bits (175), Expect = 6.460e-11 Identity = 36/75 (48.00%), Postives = 51/75 (68.00%), Query Frame = 1 Query: 46 KGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRT-*QYVGNQR*HGMGSFSLAVFSQAFTMSLGGLLEKKXG 267 KGI V GGFLI F+LGT+YTFGNMSPY+ SY++ + +++G F L++F Q +M+ GGL+EK+ G Sbjct: 8 KGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYP--DATWIFGLSIFGQGASMAFGGLIEKRIG 80
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1I8HL50 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 71.633 bits (174), Expect = 6.692e-11 Identity = 36/75 (48.00%), Postives = 51/75 (68.00%), Query Frame = 1 Query: 46 KGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRT-*QYVGNQR*HGMGSFSLAVFSQAFTMSLGGLLEKKXG 267 KGI V GGFLI F+LGT+YTFGNMSPY+ SY++ + +++G F L++F Q +M+ GGL+EK+ G Sbjct: 6 KGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYP--DATWIFGLSIFGQGASMAFGGLIEKRIG 78
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1I8H205 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 72.0182 bits (175), Expect = 7.450e-11 Identity = 36/75 (48.00%), Postives = 51/75 (68.00%), Query Frame = 1 Query: 46 KGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRT-*QYVGNQR*HGMGSFSLAVFSQAFTMSLGGLLEKKXG 267 KGI V GGFLI F+LGT+YTFGNMSPY+ SY++ + +++G F L++F Q +M+ GGL+EK+ G Sbjct: 6 KGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYP--DATWIFGLSIFGQGASMAFGGLIEKRIG 78
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060694.1 (SMESG000060694.1) HSP 1 Score: 88.5817 bits (218), Expect = 3.394e-20 Identity = 48/84 (57.14%), Postives = 55/84 (65.48%), Query Frame = 1 Query: 19 YMSETRNXXKGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRT*Q-YVGNQR*HGMGSFSLAVFSQAFTMSLGGLLEKKXG 267 Y + KGI CV G FL+ FSLGT+YTFGN+SPYIASYLKYRT ++ N H + FSL VF TMS GG LEKK G Sbjct: 4 YTKKVDRKIKGIFCVIGAFLLHFSLGTVYTFGNLSPYIASYLKYRTDNTWINNG--HLVWVFSLNVFFLGLTMSFGGFLEKKIG 85
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060698.1 (SMESG000060698.1) HSP 1 Score: 85.1149 bits (209), Expect = 4.553e-19 Identity = 45/81 (55.56%), Postives = 57/81 (70.37%), Query Frame = 1 Query: 28 ETRNXXKGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRT*Q-YVGNQR*HGMGSFSLAVFSQAFTMSLGGLLEKKXG 267 + N KGISCV G FL+ FSLGT++TFGN+SPYIASYLK RT ++ N H + FS+ VF TM+LGG+LE+K G Sbjct: 2 KVSNLVKGISCVSGAFLLHFSLGTMFTFGNLSPYIASYLKRRTSNTWITNG--HMVWVFSITVFFMGTTMTLGGILERKMG 80
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060698.1 (SMESG000060698.1) HSP 1 Score: 85.1149 bits (209), Expect = 5.037e-19 Identity = 45/81 (55.56%), Postives = 57/81 (70.37%), Query Frame = 1 Query: 28 ETRNXXKGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRT*Q-YVGNQR*HGMGSFSLAVFSQAFTMSLGGLLEKKXG 267 + N KGISCV G FL+ FSLGT++TFGN+SPYIASYLK RT ++ N H + FS+ VF TM+LGG+LE+K G Sbjct: 2 KVSNLVKGISCVSGAFLLHFSLGTMFTFGNLSPYIASYLKRRTSNTWITNG--HMVWVFSITVFFMGTTMTLGGILERKMG 80
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060699.1 (SMESG000060699.1) HSP 1 Score: 82.0333 bits (201), Expect = 5.825e-18 Identity = 47/90 (52.22%), Postives = 55/90 (61.11%), Query Frame = 1 Query: 22 MSETRNXXKGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRT*QYVGNQR*HGMGSFSLAVFSQAFTMSLGGLLEKKXGPPXXTCWI 291 M N +GISCV GGF + FSLGT YTFGN+SPY+ASYLKYRT N H + F+ + MSLGG+LEKK G TC I Sbjct: 1 MVRLGNTARGISCVTGGFFLHFSLGTFYTFGNISPYMASYLKYRTSNTWVNSG-HMVWVFAFSALFLGLIMSLGGMLEKKVG-ARITCLI 88
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060699.1 (SMESG000060699.1) HSP 1 Score: 82.0333 bits (201), Expect = 5.830e-18 Identity = 47/90 (52.22%), Postives = 55/90 (61.11%), Query Frame = 1 Query: 22 MSETRNXXKGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRT*QYVGNQR*HGMGSFSLAVFSQAFTMSLGGLLEKKXGPPXXTCWI 291 M N +GISCV GGF + FSLGT YTFGN+SPY+ASYLKYRT N H + F+ + MSLGG+LEKK G TC I Sbjct: 1 MVRLGNTARGISCVTGGFFLHFSLGTFYTFGNISPYMASYLKYRTSNTWVNSG-HMVWVFAFSALFLGLIMSLGGMLEKKVG-ARITCLI 88 The following BLAST results are available for this feature:
BLAST of MFS domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of MFS domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of MFS domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of MFS domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of MFS domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of MFS domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of MFS domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of MFS domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of MFS domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of MFS domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of MFS domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of MFS domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30014006 ID=SMED30014006|Name=MFS domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=660bpback to top Annotated Terms
The following terms have been associated with this transcript:
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