SMED30013990

Overview
NameSMED30013990
Smed IDSMED30013990
Length (bp)555
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30013990 (SMED30013990) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30013990 (SMED30013990) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30013990 (SMED30013990) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30013990 (SMED30013990) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013990

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30013990SMESG000022901.1 dd_Smed_v4_6211_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30013990SMESG000022901.1 dd_Smed_v4_6211_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30013990SMESG000022901.1 dd_Smed_v6_6211_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30013990SMESG000022901.1 Contig39877newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30013990SMESG000022901.1 Contig39877uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30013990SMESG000022901.1 dd_Smed_v4_6211_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30013990 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002849:30231..30868 -2666
Homology
[Close]
BLAST of SMED30013990 vs. Ensembl Human
Match: C5orf49 (chromosome 5 open reading frame 49 [Source:HGNC Symbol;Acc:HGNC:27028])

HSP 1 Score: 80.1073 bits (196), Expect = 6.514e-19
Identity = 48/128 (37.50%), Postives = 73/128 (57.03%), Query Frame = 3 
Query:   81 DTDEFHLYGKP-----CNLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGL 449
            +T    L GKP        SAFS++PP R  PKE +++  P      S +YD +F     Y++KLHR DR H K +GL    EE+ + V  L+SS+YG  ++  +EP +R  GR   V+++F+R+N +
Sbjct:    7 ETTASTLRGKPRPPPVSAQSAFSYIPPRRLDPKEHSYYYRPARTGIIS-LYDCIFKRRLDYDQKLHRDDREHAKSLGLHVNEEEQERPVGVLTSSVYGKRINQPIEPLNRDFGRANHVQADFYRKNDI 133
[Close]
BLAST of SMED30013990 vs. Ensembl Human
Match: C5orf49 (chromosome 5 open reading frame 49 [Source:HGNC Symbol;Acc:HGNC:27028])

HSP 1 Score: 76.2554 bits (186), Expect = 1.970e-17
Identity = 47/128 (36.72%), Postives = 72/128 (56.25%), Query Frame = 3 
Query:   81 DTDEFHLYGKP-----CNLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGL 449
            +T    L GKP        SAFS++PP R  PKE +++  P      S +YD +F     Y++KLHR DR H K +GL     E+ + V  L+SS+YG  ++  +EP +R  GR   V+++F+R+N +
Sbjct:    7 ETTASTLRGKPRPPPVSAQSAFSYIPPRRLDPKEHSYYYRPARTGIIS-LYDCIFKRRLDYDQKLHRDDREHAKSLGLH--VNEEERPVGVLTSSVYGKRINQPIEPLNRDFGRANHVQADFYRKNDI 131
[Close]
BLAST of SMED30013990 vs. Ensembl Zebrafish
Match: C19H5orf49 (si:dkeyp-46h3.1 [Source:ZFIN;Acc:ZDB-GENE-060503-559])

HSP 1 Score: 72.4034 bits (176), Expect = 3.505e-16
Identity = 41/113 (36.28%), Postives = 62/113 (54.87%), Query Frame = 3 
Query:  117 NLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGLNM 455
             LS FSF+PP R  PKE  +FN   +  ++S +YD +     G++ KLHR DR+H K  GL    EE ++  P L SS+YG +     +   R   R+  + S+F+ +NG+  
Sbjct:   14 TLSVFSFIPPRRTEPKEMRYFNCSIKAPERS-LYDCLHQSIEGFDNKLHRDDRKHAKARGLDIFSEESSRPAPVLCSSVYGRFSPLHYD-SSRSFARVAHIRSDFYNKNGITW 124
[Close]
BLAST of SMED30013990 vs. Ensembl Mouse
Match: 1700001L19Rik (RIKEN cDNA 1700001L19 gene [Source:MGI Symbol;Acc:MGI:1916565])

HSP 1 Score: 84.7297 bits (208), Expect = 1.583e-20
Identity = 49/120 (40.83%), Postives = 70/120 (58.33%), Query Frame = 3 
Query:   99 LYGKP-----CNLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRN 443
            L GKP       L AFS++PP    PKER++F+   +    S  YD VF     YN+KLHR DR H K +GL    EE+ + VP L SS+YG  ++  +EP +R  G +  V+++F+R+N
Sbjct:   48 LRGKPRPLPISALPAFSYIPPRHQGPKERSYFSREGQTGIVSL-YDCVFKRRLDYNQKLHRDDREHAKNLGLHINEEEQERTVPVLMSSVYGKRINQPIEPLNRDYGHVSHVKTDFYRKN 166
[Close]
BLAST of SMED30013990 vs. Ensembl Mouse
Match: 1700001L19Rik (RIKEN cDNA 1700001L19 gene [Source:MGI Symbol;Acc:MGI:1916565])

HSP 1 Score: 84.7297 bits (208), Expect = 1.583e-20
Identity = 49/120 (40.83%), Postives = 70/120 (58.33%), Query Frame = 3 
Query:   99 LYGKP-----CNLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRN 443
            L GKP       L AFS++PP    PKER++F+   +    S  YD VF     YN+KLHR DR H K +GL    EE+ + VP L SS+YG  ++  +EP +R  G +  V+++F+R+N
Sbjct:   48 LRGKPRPLPISALPAFSYIPPRHQGPKERSYFSREGQTGIVSL-YDCVFKRRLDYNQKLHRDDREHAKNLGLHINEEEQERTVPVLMSSVYGKRINQPIEPLNRDYGHVSHVKTDFYRKN 166
[Close]
BLAST of SMED30013990 vs. UniProt/SwissProt
Match: sp|Q9DAR0|CE049_MOUSE (Uncharacterized protein C5orf49 homolog OS=Mus musculus OX=10090 PE=1 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.107e-19
Identity = 49/120 (40.83%), Postives = 70/120 (58.33%), Query Frame = 3 
Query:   99 LYGKP-----CNLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRN 443
            L GKP       L AFS++PP    PKER++F+   +    S  YD VF     YN+KLHR DR H K +GL    EE+ + VP L SS+YG  ++  +EP +R  G +  V+++F+R+N
Sbjct:   48 LRGKPRPLPISALPAFSYIPPRHQGPKERSYFSREGQTGIVSL-YDCVFKRRLDYNQKLHRDDREHAKNLGLHINEEEQERTVPVLMSSVYGKRINQPIEPLNRDYGHVSHVKTDFYRKN 166
[Close]
BLAST of SMED30013990 vs. UniProt/SwissProt
Match: sp|A4QMS7|CE049_HUMAN (Uncharacterized protein C5orf49 OS=Homo sapiens OX=9606 GN=C5orf49 PE=1 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 3.128e-18
Identity = 48/128 (37.50%), Postives = 73/128 (57.03%), Query Frame = 3 
Query:   81 DTDEFHLYGKP-----CNLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGL 449
            +T    L GKP        SAFS++PP R  PKE +++  P      S +YD +F     Y++KLHR DR H K +GL    EE+ + V  L+SS+YG  ++  +EP +R  GR   V+++F+R+N +
Sbjct:    7 ETTASTLRGKPRPPPVSAQSAFSYIPPRRLDPKEHSYYYRPARTGIIS-LYDCIFKRRLDYDQKLHRDDREHAKSLGLHVNEEEQERPVGVLTSSVYGKRINQPIEPLNRDFGRANHVQADFYRKNDI 133
[Close]
BLAST of SMED30013990 vs. TrEMBL
Match: A0A4S2LS21 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005253 PE=4 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.033e-46
Identity = 77/135 (57.04%), Postives = 104/135 (77.04%), Query Frame = 3 
Query:   66 LHVKTDTDEFHLYGKPCNLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGLNMESGKN 470
            + V++  + FHL G+PC+LSAFS VP  R+LPKERT+FNSPK+P + S +YD+VF+     N+KLHRCDRRH K++GL    EE TK VPSLSSS YGH LD++++P +R + RI LVESEFFRRNG+++ + K+
Sbjct:   10 IDVESHGELFHLEGRPCSLSAFSHVPTGRNLPKERTYFNSPKKPDKPSCIYDAVFSGHEELNQKLHRCDRRHAKLIGLHTYQEEATKAVPSLSSSEYGHRLDNQVDPRNRSHARIMLVESEFFRRNGVDIGNTKD 144
[Close]
BLAST of SMED30013990 vs. TrEMBL
Match: G7YUP8 (Uncharacterized protein C5orf49-like protein OS=Clonorchis sinensis OX=79923 GN=CLF_111356 PE=4 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 2.112e-46
Identity = 78/135 (57.78%), Postives = 103/135 (76.30%), Query Frame = 3 
Query:   66 LHVKTDTDEFHLYGKPCNLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGLNMESGKN 470
            + V++  + FHL G+PC+LSAFS VP  R+LPKERT+FNSPK+P + S VYD+VF+     N+KLHRCDRRH K++GL    EE TK VPSLSSS YGH LD++ +P +R + RI LVESEFFRRNG+++ + K+
Sbjct:   13 IDVESHGELFHLEGRPCSLSAFSHVPTGRNLPKERTYFNSPKKPDKPSCVYDAVFSGHEELNQKLHRCDRRHAKLIGLHTYQEEATKTVPSLSSSEYGHRLDNQFDPRNRSHARIMLVESEFFRRNGVDIGNTKD 147
[Close]
BLAST of SMED30013990 vs. TrEMBL
Match: A0A3P6QF14 (Uncharacterized protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS210 PE=4 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 4.384e-46
Identity = 79/128 (61.72%), Postives = 98/128 (76.56%), Query Frame = 3 
Query:   66 LHVKTDTDEFHLYGKPCNLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGL 449
            LHVKT+ +EF L G+P  LSAFS V   RDLP+E T++N+PK+P +KSFVYD+VF+ E G+NEK  R DRRH KM+GL    EE +K +PS SSSI+GHYLD + +P DR   RIQLVESEFFRRNG+
Sbjct:   18 LHVKTEKEEFVLEGRPVMLSAFSHVAQQRDLPREYTYYNAPKKPTEKSFVYDAVFSKETGFNEKQRRDDRRHDKMVGLHIHDEEASKPIPSQSSSIFGHYLDRRCDPLDRSRVRIQLVESEFFRRNGV 145
[Close]
BLAST of SMED30013990 vs. TrEMBL
Match: A0A074ZKF6 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_06512 PE=4 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 3.580e-45
Identity = 77/135 (57.04%), Postives = 101/135 (74.81%), Query Frame = 3 
Query:   66 LHVKTDTDEFHLYGKPCNLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGLNMESGKN 470
            + V++  + FHL G+PC+LSAFS VP  R LPKERT+FNSPK+P + S VYD+VF+     N+KLHRCDRRH K++GL    EE TK VPSLSSS YGH L ++ +P +R + RI LVESEFFRRNG+++ + K+
Sbjct:   10 IDVESHGELFHLEGRPCSLSAFSHVPTGRSLPKERTYFNSPKKPDKPSCVYDAVFSGHEELNQKLHRCDRRHAKLIGLHTYEEEATKAVPSLSSSEYGHRLGNQFDPRNRSHARIMLVESEFFRRNGVDIGNTKD 144
[Close]
BLAST of SMED30013990 vs. TrEMBL
Match: A0A5J4NKY1 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0014685 PE=4 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.040e-44
Identity = 75/139 (53.96%), Postives = 101/139 (72.66%), Query Frame = 3 
Query:   39 VDTSYGRVHLHVKTDTDEFHLYGKPCNLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGLNM 455
            + ++Y  V + V++  + FHL GKPC LSAFS+VP  R LPKERT+FNSPK+P     VYD+VF+ E   N K+HR DRRH K++GL    EE TK VP+LSSS+YGH L+++ +P DR + RI LVESEF+RRN +++
Sbjct:    1 MSSAYELVTIDVESHGENFHLEGKPCALSAFSYVPTSRSLPKERTYFNSPKKPDNARCVYDAVFSKEEELNPKIHRSDRRHDKLIGLHTNDEEVTKAVPTLSSSLYGHRLENQYDPRDRSHARIMLVESEFYRRNKIDI 139
[Close]
BLAST of SMED30013990 vs. Ensembl Cavefish
Match: si:dkeyp-46h3.1 (chromosome 23 C5orf49 homolog [Source:NCBI gene;Acc:103039620])

HSP 1 Score: 62.3882 bits (150), Expect = 1.560e-12
Identity = 47/113 (41.59%), Postives = 66/113 (58.41%), Query Frame = 3 
Query:  117 NLSAFSFVPPDRDLPKERTFFN-SPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGLN 452
             LS FSF+PP R+ P+ER +F  SP+ P  + ++YD       GY+ KLHR DR+H K  GL    EE ++ +P  SSS YG  L   L    R+  R+  + SEF+R+NG+ 
Sbjct:   14 TLSVFSFIPPRRNEPRERRYFYCSPQAP--EVYLYDCTHRRAEGYDNKLHRDDRQHAKGHGLDIFSEESSRPIPVRSSSDYGRRLLPPLYKPGRQFVRVAHIRSEFYRKNGIT 124
[Close]
BLAST of SMED30013990 vs. Ensembl Nematostella
Match: EDO43713 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXS2])

HSP 1 Score: 67.781 bits (164), Expect = 4.126e-15
Identity = 36/87 (41.38%), Postives = 51/87 (58.62%), Query Frame = 3 
Query:  213 VYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGLNMESGKNV 473
             YD +  +  GYN+KLHR DR H K  GL    EE   +VP LSSS+YGH     LE +DR + R++L + +F R  G ++ +  N+
Sbjct:   39 TYDRINTVALGYNQKLHRDDREHAKSRGLRVNDEETAVRVPVLSSSVYGHPARRPLEFQDRTHVRVELCKKDFLRLGGTDIGNNPNL 125
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BLAST of SMED30013990 vs. Ensembl Nematostella
Match: EDO45922 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRC0])

HSP 1 Score: 53.5286 bits (127), Expect = 2.687e-9
Identity = 34/95 (35.79%), Postives = 54/95 (56.84%), Query Frame = 3 
Query:  171 TFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGLNM 455
            ++FN+ K+ + +   YD +F ++ GY  KLHR DR+H     +    EE  K VP L+SS YG      LE   R++ R+  V+ +F+R +G N+
Sbjct:   83 SYFNNGKQ-QTRVLTYDRMFKIKQGYCSKLHRDDRQHTDAFDV--HAEECEKAVPVLTSSQYGRRP--PLETPQRKHVRVATVKRDFYRYSGTNI 172
[Close]
BLAST of SMED30013990 vs. Planmine SMEST
Match: SMESG000022901.1 (SMESG000022901.1)

HSP 1 Score: 308.916 bits (790), Expect = 2.388e-109
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 3 
Query:   36 MVDTSYGRVHLHVKTDTDEFHLYGKPCNLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGLNMESGKNVAK 479
            MVDTSYGRVHLHVKTDTDEFHLYGKPCNLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGLNMESGKNVAK
Sbjct:    1 MVDTSYGRVHLHVKTDTDEFHLYGKPCNLSAFSFVPPDRDLPKERTFFNSPKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVPSLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGLNMESGKNVAK 148
The following BLAST results are available for this feature:
BLAST of SMED30013990 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
C5orf496.514e-1937.50chromosome 5 open reading frame 49 [Source:HGNC Sy... [more]
C5orf491.970e-1736.72chromosome 5 open reading frame 49 [Source:HGNC Sy... [more]
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BLAST of SMED30013990 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013990 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013990 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
C19H5orf493.505e-1636.28si:dkeyp-46h3.1 [Source:ZFIN;Acc:ZDB-GENE-060503-5... [more]
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BLAST of SMED30013990 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013990 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
1700001L19Rik1.583e-2040.83RIKEN cDNA 1700001L19 gene [Source:MGI Symbol;Acc:... [more]
1700001L19Rik1.583e-2040.83RIKEN cDNA 1700001L19 gene [Source:MGI Symbol;Acc:... [more]
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BLAST of SMED30013990 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q9DAR0|CE049_MOUSE1.107e-1940.83Uncharacterized protein C5orf49 homolog OS=Mus mus... [more]
sp|A4QMS7|CE049_HUMAN3.128e-1837.50Uncharacterized protein C5orf49 OS=Homo sapiens OX... [more]
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BLAST of SMED30013990 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2LS211.033e-4657.04Uncharacterized protein OS=Opisthorchis felineus O... [more]
G7YUP82.112e-4657.78Uncharacterized protein C5orf49-like protein OS=Cl... [more]
A0A3P6QF144.384e-4661.72Uncharacterized protein OS=Dibothriocephalus latus... [more]
A0A074ZKF63.580e-4557.04Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A5J4NKY11.040e-4453.96Uncharacterized protein OS=Paragonimus westermani ... [more]
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BLAST of SMED30013990 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
si:dkeyp-46h3.11.560e-1241.59chromosome 23 C5orf49 homolog [Source:NCBI gene;Ac... [more]
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BLAST of SMED30013990 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013990 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013990 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO437134.126e-1541.38Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO459222.687e-935.79Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30013990 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013990 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000022901.12.388e-109100.00SMESG000022901.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013990 ID=SMED30013990|Name=SMED30013990|organism=Schmidtea mediterranea sexual|type=transcript|length=555bp
GTTTAAACTCATTTATAGTACTCTAAAATTTTGAAATGGTAGATACCAGC
TATGGAAGAGTTCACCTTCATGTAAAAACCGACACTGATGAATTTCATCT
TTATGGCAAACCATGCAATTTATCAGCATTTTCTTTTGTTCCCCCTGATC
GTGATTTGCCTAAAGAAAGAACTTTCTTCAATTCTCCTAAAGAGCCAAGA
CAAAAATCATTTGTTTACGATTCAGTATTCGCCCTAGAATATGGTTACAA
TGAAAAATTGCATAGATGTGATCGAAGACATCACAAAATGATGGGTCTCT
GTCGAATTTGCGAAGAGAAAACGAAAAAAGTCCCGTCTTTATCATCATCA
ATTTACGGGCATTATCTTGATCATAAACTGGAGCCAGAAGATCGACGAAA
CGGCAGAATTCAGTTGGTTGAAAGTGAATTTTTCCGCCGAAATGGCCTGA
ATATGGAATCCGGGAAAAATGTAGCTAAATAATTGACATAGATTTCCCGA
TAATTTCATAAATTATTTACTAGAGTAAATAAATTATTTTACCGCTAAAA
AAAAA
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protein sequence of SMED30013990-orf-1

>SMED30013990-orf-1 ID=SMED30013990-orf-1|Name=SMED30013990-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=149bp
MVDTSYGRVHLHVKTDTDEFHLYGKPCNLSAFSFVPPDRDLPKERTFFNS
PKEPRQKSFVYDSVFALEYGYNEKLHRCDRRHHKMMGLCRICEEKTKKVP
SLSSSIYGHYLDHKLEPEDRRNGRIQLVESEFFRRNGLNMESGKNVAK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000418head
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR027901DUF4541
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027901Protein of unknown function DUF4541PFAMPF15074DUF4541coord: 46..137
e-value: 4.8E-27
score: 94.4
IPR027901Protein of unknown function DUF4541PANTHERPTHR34444FAMILY NOT NAMEDcoord: 28..145