Akirin-1

Overview
NameAkirin-1
Smed IDSMED30013894
Length (bp)738
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Akirin-1 (SMED30013894) t-SNE clustered cells

Violin plots show distribution of expression levels for Akirin-1 (SMED30013894) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Akirin-1 (SMED30013894) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Akirin-1 (SMED30013894) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013894

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30013894h1SMcG0008498 SmedASXL_016528SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Akirin-1 vs. Ensembl Human
Match: AKIRIN1 (akirin 1 [Source:HGNC Symbol;Acc:HGNC:25744])

HSP 1 Score: 55.8398 bits (133), Expect = 4.761e-9
Identity = 24/75 (32.00%), Postives = 47/75 (62.67%), Query Frame = 3 
Query:  396 DSDASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            +S   S++L   +S   S   ++ P ++L +   IC  ++K+ E  +++E+ ++L+ KL+EQY++FVKF  D I+
Sbjct:  106 ESQPHSSALTAPSSPGSSWMKKDQPTFTLRQVGIICERLLKDYEDKIREEYEQILNTKLAEQYESFVKFTHDQIM 180
[Close]
BLAST of Akirin-1 vs. Ensembl Human
Match: AKIRIN1 (akirin 1 [Source:HGNC Symbol;Acc:HGNC:25744])

HSP 1 Score: 51.2174 bits (121), Expect = 1.346e-7
Identity = 37/158 (23.42%), Postives = 68/158 (43.04%), Query Frame = 3 
Query:  150 MAC-STLKRPCEIEDVENSNLRYMYPYSLKRHSDITEFTNPSKKMCLYSDHQEKRSLYVSQSTIYHQLHTIEEVQIPNEKEEFDSDASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            MAC +TLKRP E E         + P S KR         P+  +      +   +             ++++   P  +    +  SS               ++ P ++L +   IC  ++K+ E  +++E+ ++L+ KL+EQY++FVKF  D I+
Sbjct:    1 MACGATLKRPMEFEAA------LLSPGSPKRRR-CAPLPGPTPGL------RPPDAEPPPPFQTQTPPQSLQQPAPPGSERRLPTPGSS------------WMKKDQPTFTLRQVGIICERLLKDYEDKIREEYEQILNTKLAEQYESFVKFTHDQIM 133
[Close]
BLAST of Akirin-1 vs. Ensembl Fly
Match: akirin (gene:FBgn0082598 transcript:FBtr0332730)

HSP 1 Score: 46.595 bits (109), Expect = 4.779e-6
Identity = 21/40 (52.50%), Postives = 27/40 (67.50%), Query Frame = 3 
Query:  498 ICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWI 617
            IC  MIK  E+ L++ +  VL+ KL+EQYD FVKF  D I
Sbjct:  150 ICESMIKERENQLRERYESVLTTKLAEQYDAFVKFTYDQI 189
[Close]
BLAST of Akirin-1 vs. Ensembl Fly
Match: akirin (gene:FBgn0082598 transcript:FBtr0076848)

HSP 1 Score: 46.595 bits (109), Expect = 4.779e-6
Identity = 21/40 (52.50%), Postives = 27/40 (67.50%), Query Frame = 3 
Query:  498 ICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWI 617
            IC  MIK  E+ L++ +  VL+ KL+EQYD FVKF  D I
Sbjct:  150 ICESMIKERENQLRERYESVLTTKLAEQYDAFVKFTYDQI 189
[Close]
BLAST of Akirin-1 vs. Ensembl Fly
Match: akirin (gene:FBgn0082598 transcript:FBtr0302544)

HSP 1 Score: 46.595 bits (109), Expect = 4.779e-6
Identity = 21/40 (52.50%), Postives = 27/40 (67.50%), Query Frame = 3 
Query:  498 ICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWI 617
            IC  MIK  E+ L++ +  VL+ KL+EQYD FVKF  D I
Sbjct:  150 ICESMIKERENQLRERYESVLTTKLAEQYDAFVKFTYDQI 189
[Close]
BLAST of Akirin-1 vs. Ensembl Fly
Match: akirin (gene:FBgn0082598 transcript:FBtr0302545)

HSP 1 Score: 46.595 bits (109), Expect = 4.779e-6
Identity = 21/40 (52.50%), Postives = 27/40 (67.50%), Query Frame = 3 
Query:  498 ICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWI 617
            IC  MIK  E+ L++ +  VL+ KL+EQYD FVKF  D I
Sbjct:  150 ICESMIKERENQLRERYESVLTTKLAEQYDAFVKFTYDQI 189
[Close]
BLAST of Akirin-1 vs. Ensembl Fly
Match: akirin (gene:FBgn0082598 transcript:FBtr0302543)

HSP 1 Score: 46.595 bits (109), Expect = 4.779e-6
Identity = 21/40 (52.50%), Postives = 27/40 (67.50%), Query Frame = 3 
Query:  498 ICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWI 617
            IC  MIK  E+ L++ +  VL+ KL+EQYD FVKF  D I
Sbjct:  150 ICESMIKERENQLRERYESVLTTKLAEQYDAFVKFTYDQI 189
[Close]
BLAST of Akirin-1 vs. Ensembl Zebrafish
Match: akirin1 (akirin 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2178])

HSP 1 Score: 53.9138 bits (128), Expect = 1.536e-8
Identity = 25/76 (32.89%), Postives = 49/76 (64.47%), Query Frame = 3 
Query:  396 DSDASSTSLCTETSK-NQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            D  +SS +L + +S    S   ++ P ++L +   +C  +IK+ E  +++E+ ++L+ KL+EQY++FVKF +D I+
Sbjct:  102 DIQSSSPALTSPSSPTGASSLKKDQPLFTLRQVGYLCERLIKDHEEKMREEYEQILNTKLAEQYESFVKFTQDQIM 177
[Close]
BLAST of Akirin-1 vs. Ensembl Zebrafish
Match: akirin1 (akirin 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2178])

HSP 1 Score: 53.5286 bits (127), Expect = 2.591e-8
Identity = 25/76 (32.89%), Postives = 49/76 (64.47%), Query Frame = 3 
Query:  396 DSDASSTSLCTETSK-NQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            D  +SS +L + +S    S   ++ P ++L +   +C  +IK+ E  +++E+ ++L+ KL+EQY++FVKF +D I+
Sbjct:  124 DIQSSSPALTSPSSPTGASSLKKDQPLFTLRQVGYLCERLIKDHEEKMREEYEQILNTKLAEQYESFVKFTQDQIM 199
[Close]
BLAST of Akirin-1 vs. Ensembl Xenopus
Match: pou2f3 (POU class 2 homeobox 3 [Source:Xenbase;Acc:XB-GENE-853632])

HSP 1 Score: 53.9138 bits (128), Expect = 2.460e-8
Identity = 26/72 (36.11%), Postives = 47/72 (65.28%), Query Frame = 3 
Query:  405 ASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            AS +SL T  S   S+  ++ P +SL +   +C  ++K+ E  +++E+ ++L+ KL+EQY++FVKF  D I+
Sbjct:  104 ASCSSL-TAPSSPGSLVKKDQPTFSLRQVGILCERLLKDHEDKIREEYEQILNIKLAEQYESFVKFTHDQIM 174
[Close]
BLAST of Akirin-1 vs. Ensembl Xenopus
Match: zbtb12 (zinc finger and BTB domain containing 12 [Source:Xenbase;Acc:XB-GENE-481210])

HSP 1 Score: 51.9878 bits (123), Expect = 9.376e-8
Identity = 21/51 (41.18%), Postives = 34/51 (66.67%), Query Frame = 3 
Query:  468 PKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            P +SL +   IC  ++K  E  +++E+ ++L+ KL+EQYD FVKF  D I+
Sbjct:  119 PLFSLRQVGMICERLLKEREDKVREEYEEILTTKLAEQYDAFVKFTHDQIM 169
[Close]
BLAST of Akirin-1 vs. Ensembl Mouse
Match: Akirin1 (akirin 1 [Source:MGI Symbol;Acc:MGI:1915300])

HSP 1 Score: 56.6102 bits (135), Expect = 2.012e-9
Identity = 44/186 (23.66%), Postives = 78/186 (41.94%), Query Frame = 3 
Query:  150 MAC-STLKRPCEIEDVENSNLRYMYPYSLKRHSDITEFTNPSKKMCLYSDHQEKRSLYVSQSTIYHQLHTIEEVQIP-------NEKEEF---------------------DSDASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            MAC +TLKRP E E         + P S KR         P+  +           +    +++        E ++P       N K+E+                     ++  SS++L    S       ++ P ++L +   IC  ++K+ E  +++E+ ++LS KL+EQY++FVKF  D I+
Sbjct:    1 MACGATLKRPMEFEAA------LLSPGSPKRRR-CAPLPGPTPGLRPPDAEPPPLQMQTPPASLQQPAPPGSERRLPTPEQIFQNIKQEYNRYQRWRHLEVVLSQSEACTSETQPSSSALTAPGSPGAFWMKKDQPTFTLRQVGIICERLLKDYEDKVREEYEQILSTKLAEQYESFVKFTHDQIM 179
[Close]
BLAST of Akirin-1 vs. UniProt/SwissProt
Match: sp|Q99LF1|AKIR1_MOUSE (Akirin-1 OS=Mus musculus OX=10090 GN=Akirin1 PE=1 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.408e-8
Identity = 44/186 (23.66%), Postives = 78/186 (41.94%), Query Frame = 3 
Query:  150 MAC-STLKRPCEIEDVENSNLRYMYPYSLKRHSDITEFTNPSKKMCLYSDHQEKRSLYVSQSTIYHQLHTIEEVQIP-------NEKEEF---------------------DSDASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            MAC +TLKRP E E         + P S KR         P+  +           +    +++        E ++P       N K+E+                     ++  SS++L    S       ++ P ++L +   IC  ++K+ E  +++E+ ++LS KL+EQY++FVKF  D I+
Sbjct:    1 MACGATLKRPMEFEAA------LLSPGSPKRRR-CAPLPGPTPGLRPPDAEPPPLQMQTPPASLQQPAPPGSERRLPTPEQIFQNIKQEYNRYQRWRHLEVVLSQSEACTSETQPSSSALTAPGSPGAFWMKKDQPTFTLRQVGIICERLLKDYEDKVREEYEQILSTKLAEQYESFVKFTHDQIM 179
[Close]
BLAST of Akirin-1 vs. UniProt/SwissProt
Match: sp|Q9H9L7|AKIR1_HUMAN (Akirin-1 OS=Homo sapiens OX=9606 GN=AKIRIN1 PE=1 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 2.286e-8
Identity = 24/75 (32.00%), Postives = 47/75 (62.67%), Query Frame = 3 
Query:  396 DSDASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            +S   S++L   +S   S   ++ P ++L +   IC  ++K+ E  +++E+ ++L+ KL+EQY++FVKF  D I+
Sbjct:  106 ESQPHSSALTAPSSPGSSWMKKDQPTFTLRQVGIICERLLKDYEDKIREEYEQILNTKLAEQYESFVKFTHDQIM 180
[Close]
BLAST of Akirin-1 vs. UniProt/SwissProt
Match: sp|Q5FWN7|AKIRA_XENLA (Akirin-1A OS=Xenopus laevis OX=8355 GN=akirin1-a PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 1.114e-7
Identity = 26/72 (36.11%), Postives = 47/72 (65.28%), Query Frame = 3 
Query:  405 ASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            AS +SL T  S   S+  ++ P +SL +   +C  ++K+ E  +++E+ ++L+ KL+EQY++FVKF  D I+
Sbjct:  104 ASCSSL-TAPSSPGSLVKKDQPTFSLRQVGILCERLLKDHEDKIREEYEQILNIKLAEQYESFVKFTHDQIM 174
[Close]
BLAST of Akirin-1 vs. UniProt/SwissProt
Match: sp|Q7ZY70|AKIRB_XENLA (Akirin-1B OS=Xenopus laevis OX=8355 GN=akirin1-b PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 1.191e-7
Identity = 26/72 (36.11%), Postives = 47/72 (65.28%), Query Frame = 3 
Query:  405 ASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            AS +SL T  S   S+  ++ P +SL +   +C  ++K+ E  +++E+ ++L+ KL+EQY++FVKF  D I+
Sbjct:  101 ASCSSL-TAPSSPGSLVKKDQPTFSLRQVGILCERLLKDHEDKIREEYEQILNIKLAEQYESFVKFTHDQIM 171
[Close]
BLAST of Akirin-1 vs. UniProt/SwissProt
Match: sp|Q5BL57|AKIR1_XENTR (Akirin-1 OS=Xenopus tropicalis OX=8364 GN=akirin1 PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 1.303e-7
Identity = 26/72 (36.11%), Postives = 47/72 (65.28%), Query Frame = 3 
Query:  405 ASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            AS +SL T  S   S+  ++ P +SL +   +C  ++K+ E  +++E+ ++L+ KL+EQY++FVKF  D I+
Sbjct:  104 ASCSSL-TAPSSPGSLVKKDQPTFSLRQVGILCERLLKDHEDKIREEYEQILNIKLAEQYESFVKFTHDQIM 174
[Close]
BLAST of Akirin-1 vs. TrEMBL
Match: A0A2J7QQW9 (Akirin OS=Cryptotermes secundus OX=105785 GN=akirin PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 9.865e-8
Identity = 48/169 (28.40%), Postives = 80/169 (47.34%), Query Frame = 3 
Query:  150 MACSTLKRPCEIEDVEN----SNLRYMYPYSLKRHSDIT-EFTNPSKKMC--------LYSDHQEKRSLYVSQSTIYHQLHTIEEVQIPNEKEEFDSDASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWI 617
            MAC+TLKR  + + V N    S  R   P  +  +   T +  +P  ++C          +  +E R L+  +   ++    +   Q  +     DS  S T+    T+ + S  ++  P ++  +   IC  M+K  ES +++E+ +VL+ KLSEQYD FVKF  D I
Sbjct:    1 MACATLKRTLDFDPVHNHGRPSKRRRCAPVCISPNVPSTKQHPSPFGEVCPKLTPEKMAANIREEIRRLHRRKQLSFYPGQDVHGSQESSSDGAMDSPGSPTTSSFATTYSSS--TKEKPLFTFRQVGLICERMLKERESQIREEYDRVLNTKLSEQYDTFVKFTYDQI 167
[Close]
BLAST of Akirin-1 vs. TrEMBL
Match: A0A2P6JZL5 (Akirin OS=Nephila clavipes OX=2585209 GN=NCL1_57131 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.250e-7
Identity = 49/167 (29.34%), Postives = 83/167 (49.70%), Query Frame = 3 
Query:  150 MACSTLKRPCEIEDVENSNLRYMYPYSLKRHSDITEFTNPSKKMCLYSDHQEKRSLYVSQSTIYHQL--HTIEEVQIPNEKEEFDSDASS------TSLCTETSKNQS---VFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWI 617
            MAC+TLKR CE + +++ N R     S KR    T    PS K+   S   +     V+      Q+  +  EE++  + +++   DAS        +  T +S+ +    +   + P ++  +   IC  MIK+ E+ ++ E+ ++L+ KL+EQYD FVKF  D I
Sbjct:    1 MACATLKRSCEWDPLQSPNGR-----SPKRRR-CTPVMAPSPKIFKSSTENQSPFGEVASKLTSEQIAENIREEIKKLHRRKQLKFDASESSDSSSGTPATTSSRAEGSGLLACRDQPLFTFRQVGLICERMIKDRENQIQLEYDRILNAKLAEQYDTFVKFTYDQI 161
[Close]
BLAST of Akirin-1 vs. TrEMBL
Match: A0A067R299 (Akirin OS=Zootermopsis nevadensis OX=136037 GN=L798_14242 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.489e-7
Identity = 53/175 (30.29%), Postives = 82/175 (46.86%), Query Frame = 3 
Query:  150 MACSTLKRPCEIEDVEN----SNLRYMYPYSLKRHSDITEF--TNPS--KKMC--------LYSDHQEKRSLYVSQSTIYHQLHTIEEVQIPNEKEEFDSDASSTSLCTETSKNQSVFSENI---PKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWI 617
            MAC+TLKR  + + V N    S  R   P  +  ++  T+    NPS   ++C          S  +E R L+  +   ++  H +   Q  +     +S  S T     TS   + +S N    P ++  +   IC  M+K  ES +++E+ +VL+ KLSEQYD FVKF  D I
Sbjct:    1 MACATLKRTLDFDPVHNHGRPSKRRRCVPMCMSPNTPPTKQHELNPSPFGEVCPKLTPEKMAASIREEIRRLHRRKQLNFYPGHEVHCHQESSSDGAMESPGSPT-----TSSFATTYSSNTKEKPLFTFRQVGLICERMLKERESQIREEYDRVLNAKLSEQYDAFVKFTYDQI 170
[Close]
BLAST of Akirin-1 vs. TrEMBL
Match: T1JC90 (Uncharacterized protein OS=Strigamia maritima OX=126957 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.664e-7
Identity = 52/170 (30.59%), Postives = 81/170 (47.65%), Query Frame = 3 
Query:  150 MACSTLKRPCEIEDVENSNLRYMYPYSLKRHSDIT--EFTNPSKKMCLYSDHQEKRSLY--VSQSTIYHQLHT--IEEVQIPNEKEEFDSDASSTSLCTET--------SKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWI 617
            MAC+TLKRP E + + + + R       +R   IT    + P+K       H+   S +  V+Q     Q+ T   EE++    +++    ASS S+ TE+        +   S    + P ++  +   IC  M+K  E  +K ++ +VL  KLSEQYD FVKF  D I
Sbjct:    1 MACATLKRPLEFDHLHSPSQRA----KRRRCMPITMSPTSAPTKA------HELNPSPFDSVAQKLSPDQIATNVREEIRRLQRRKQLHFSASSESMTTESPASPMSVVNAGASTSKRDQPLFTFRQVGLICERMLKEREDQIKGQYDEVLMTKLSEQYDTFVKFTYDQI 160
[Close]
BLAST of Akirin-1 vs. TrEMBL
Match: C3YH63 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_115111 PE=4 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 4.397e-7
Identity = 24/58 (41.38%), Postives = 43/58 (74.14%), Query Frame = 3 
Query:  444 QSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWI 617
            QS  +++ P +S+ + + IC  MIK  ++ +++E+ KVLS KL+EQYD F++FN+D++
Sbjct:  145 QSPINKDKPLFSMRQVSMICERMIKERDTQVREEYDKVLSCKLAEQYDAFIRFNQDYL 202
[Close]
BLAST of Akirin-1 vs. Ensembl Cavefish
Match: akirin1 (akirin 1 [Source:NCBI gene;Acc:103036380])

HSP 1 Score: 50.447 bits (119), Expect = 2.594e-7
Identity = 18/51 (35.29%), Postives = 37/51 (72.55%), Query Frame = 3 
Query:  468 PKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            P ++L +   +C  ++K+ E  +++E+ ++L+ KL+EQY++FVKF +D I+
Sbjct:  128 PTFTLRQVGYLCERLLKDHEEKIREEYEQILNTKLAEQYESFVKFTQDQIM 178
[Close]
BLAST of Akirin-1 vs. Ensembl Sea Lamprey
Match: akirin2 (akirin 2 [Source:ZFIN;Acc:ZDB-GENE-040426-2944])

HSP 1 Score: 57.3806 bits (137), Expect = 1.550e-10
Identity = 26/94 (27.66%), Postives = 51/94 (54.26%), Query Frame = 3 
Query:  339 YHQLHTIEEVQIPNEKEEFDSDASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            Y ++    +V  P +    DS +  +   + TS +        P ++L +   IC+ +++  E  +++E+ ++LS KL+EQY+ FVKF +D I+
Sbjct:   82 YSRMQRRLQVDGPPDAHAGDSASMQSGPASPTSVSGDGGRREQPLFTLRQVGLICDGLLREREVHIRQEYDQILSTKLAEQYEGFVKFTQDQIM 175
[Close]
BLAST of Akirin-1 vs. Ensembl Nematostella
Match: EDO33853 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQY7])

HSP 1 Score: 56.6102 bits (135), Expect = 4.294e-10
Identity = 23/50 (46.00%), Postives = 38/50 (76.00%), Query Frame = 3 
Query:  468 PKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWI 617
            P ++L + + +C  M+K  ++ L++E+ KVL++KLSEQYD+FVKFN D +
Sbjct:  105 PLFTLKQVSLLCERMLKERDTQLREEYDKVLAQKLSEQYDSFVKFNYDQV 154
[Close]
BLAST of Akirin-1 vs. Ensembl Medaka
Match: akirin1 (akirin 1 [Source:NCBI gene;Acc:101154806])

HSP 1 Score: 51.2174 bits (121), Expect = 1.052e-7
Identity = 20/65 (30.77%), Postives = 42/65 (64.62%), Query Frame = 3 
Query:  426 TETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            T  S       ++ P ++L + + +C  ++K+ E  +++E+ ++L+ KL+EQY++FVKF +D I+
Sbjct:  111 TAPSSPPGASRKDQPSFTLRQVSLLCERLLKDHEEKIREEYEQILNTKLAEQYESFVKFTQDQIM 175
[Close]
BLAST of Akirin-1 vs. Ensembl Medaka
Match: akirin1 (akirin 1 [Source:NCBI gene;Acc:101154806])

HSP 1 Score: 51.2174 bits (121), Expect = 1.052e-7
Identity = 20/65 (30.77%), Postives = 42/65 (64.62%), Query Frame = 3 
Query:  426 TETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            T  S       ++ P ++L + + +C  ++K+ E  +++E+ ++L+ KL+EQY++FVKF +D I+
Sbjct:  111 TAPSSPPGASRKDQPSFTLRQVSLLCERLLKDHEEKIREEYEQILNTKLAEQYESFVKFTQDQIM 175
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BLAST of Akirin-1 vs. Ensembl Medaka
Match: akirin2 (akirin 2 [Source:NCBI gene;Acc:101162258])

HSP 1 Score: 49.2914 bits (116), Expect = 5.131e-7
Identity = 20/51 (39.22%), Postives = 33/51 (64.71%), Query Frame = 3 
Query:  468 PKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWIL 620
            P +SL +   IC  ++K  E  +++E+ + L+ KL+EQYD FVKF  D ++
Sbjct:  117 PLFSLRQVGMICERLLKEREDKIREEYDETLTTKLAEQYDAFVKFTHDQLM 167
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BLAST of Akirin-1 vs. Planmine SMEST
Match: SMESG000066833.1 (SMESG000066833.1)

HSP 1 Score: 341.658 bits (875), Expect = 7.382e-121
Identity = 166/168 (98.81%), Postives = 168/168 (100.00%), Query Frame = 3 
Query:  150 MACSTLKRPCEIEDVENSNLRYMYPYSLKRHSDITEFTNPSKKMCLYSDHQEKRSLYVSQSTIYHQLHTIEEVQIPNEKEEFDSDASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWILPPLDSDCWYVC 653
            MACSTLKRPCEIEDVENSNLRYMYPYSLKRHSDITEFTNPSKKMCLYSDHQEKRSLYVSQSTIYHQLHTIEEVQIPNEK+EFD+DASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWILPPLDSDCWYVC
Sbjct:    1 MACSTLKRPCEIEDVENSNLRYMYPYSLKRHSDITEFTNPSKKMCLYSDHQEKRSLYVSQSTIYHQLHTIEEVQIPNEKDEFDNDASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWILPPLDSDCWYVC 168
[Close]
BLAST of Akirin-1 vs. Planmine SMEST
Match: SMESG000066824.1 (SMESG000066824.1)

HSP 1 Score: 341.273 bits (874), Expect = 9.091e-121
Identity = 166/168 (98.81%), Postives = 168/168 (100.00%), Query Frame = 3 
Query:  150 MACSTLKRPCEIEDVENSNLRYMYPYSLKRHSDITEFTNPSKKMCLYSDHQEKRSLYVSQSTIYHQLHTIEEVQIPNEKEEFDSDASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWILPPLDSDCWYVC 653
            MACSTLKRPCEIEDVENSNLRYMYPYSLKRHSDITEFTNPSKK+CLYSDHQEKRSLYVSQSTIYHQLHTIEEVQIPNEK+EFDSDASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWILPPLDSDCWYVC
Sbjct:    1 MACSTLKRPCEIEDVENSNLRYMYPYSLKRHSDITEFTNPSKKICLYSDHQEKRSLYVSQSTIYHQLHTIEEVQIPNEKDEFDSDASSTSLCTETSKNQSVFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVKFNKDWILPPLDSDCWYVC 168
The following BLAST results are available for this feature:
BLAST of Akirin-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
AKIRIN14.761e-932.00akirin 1 [Source:HGNC Symbol;Acc:HGNC:25744][more]
AKIRIN11.346e-723.42akirin 1 [Source:HGNC Symbol;Acc:HGNC:25744][more]
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BLAST of Akirin-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Akirin-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
akirin4.779e-652.50gene:FBgn0082598 transcript:FBtr0332730[more]
akirin4.779e-652.50gene:FBgn0082598 transcript:FBtr0076848[more]
akirin4.779e-652.50gene:FBgn0082598 transcript:FBtr0302544[more]
akirin4.779e-652.50gene:FBgn0082598 transcript:FBtr0302545[more]
akirin4.779e-652.50gene:FBgn0082598 transcript:FBtr0302543[more]
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BLAST of Akirin-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
akirin11.536e-832.89akirin 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2178][more]
akirin12.591e-832.89akirin 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2178][more]
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BLAST of Akirin-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pou2f32.460e-836.11POU class 2 homeobox 3 [Source:Xenbase;Acc:XB-GENE... [more]
zbtb129.376e-841.18zinc finger and BTB domain containing 12 [Source:X... [more]
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BLAST of Akirin-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Akirin12.012e-923.66akirin 1 [Source:MGI Symbol;Acc:MGI:1915300][more]
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BLAST of Akirin-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q99LF1|AKIR1_MOUSE1.408e-823.66Akirin-1 OS=Mus musculus OX=10090 GN=Akirin1 PE=1 ... [more]
sp|Q9H9L7|AKIR1_HUMAN2.286e-832.00Akirin-1 OS=Homo sapiens OX=9606 GN=AKIRIN1 PE=1 S... [more]
sp|Q5FWN7|AKIRA_XENLA1.114e-736.11Akirin-1A OS=Xenopus laevis OX=8355 GN=akirin1-a P... [more]
sp|Q7ZY70|AKIRB_XENLA1.191e-736.11Akirin-1B OS=Xenopus laevis OX=8355 GN=akirin1-b P... [more]
sp|Q5BL57|AKIR1_XENTR1.303e-736.11Akirin-1 OS=Xenopus tropicalis OX=8364 GN=akirin1 ... [more]
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BLAST of Akirin-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2J7QQW99.865e-828.40Akirin OS=Cryptotermes secundus OX=105785 GN=akiri... [more]
A0A2P6JZL51.250e-729.34Akirin OS=Nephila clavipes OX=2585209 GN=NCL1_5713... [more]
A0A067R2991.489e-730.29Akirin OS=Zootermopsis nevadensis OX=136037 GN=L79... [more]
T1JC901.664e-730.59Uncharacterized protein OS=Strigamia maritima OX=1... [more]
C3YH634.397e-741.38Uncharacterized protein OS=Branchiostoma floridae ... [more]
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BLAST of Akirin-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
akirin12.594e-735.29akirin 1 [Source:NCBI gene;Acc:103036380][more]
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BLAST of Akirin-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
akirin21.550e-1027.66akirin 2 [Source:ZFIN;Acc:ZDB-GENE-040426-2944][more]
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BLAST of Akirin-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Akirin-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO338534.294e-1046.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Akirin-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
akirin11.052e-730.77akirin 1 [Source:NCBI gene;Acc:101154806][more]
akirin11.052e-730.77akirin 1 [Source:NCBI gene;Acc:101154806][more]
akirin25.131e-739.22akirin 2 [Source:NCBI gene;Acc:101162258][more]
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BLAST of Akirin-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000066833.17.382e-12198.81SMESG000066833.1[more]
SMESG000066824.19.091e-12198.81SMESG000066824.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013894 ID=SMED30013894|Name=Akirin-1|organism=Schmidtea mediterranea sexual|type=transcript|length=738bp
GAGGAATCGCAATTAATTTCCATTGGGGAATCCTATTGTAGGAAGCTGTT
TCTAGGAAGCCGTTTCTAGGAATCCGGGTTGAATTCGAAAAATTTGTTTA
AATTTATAAATTTTTATATTATTTAAATTTGCAATTTTATTTGAGATAAA
TGGCTTGTTCTACATTGAAAAGACCCTGTGAAATAGAAGATGTTGAGAAT
TCCAATCTAAGATATATGTATCCTTATTCATTAAAAAGGCATTCTGATAT
TACCGAGTTTACTAATCCTTCGAAAAAAATGTGCTTATATTCTGATCATC
AAGAAAAAAGAAGTTTATATGTGTCACAGTCAACTATTTATCATCAACTT
CATACAATTGAAGAAGTTCAAATACCAAATGAAAAAGAGGAATTTGACAG
TGATGCATCATCTACGTCATTATGCACAGAAACATCTAAAAATCAGTCAG
TTTTTAGTGAGAACATTCCCAAATATAGTCTAACTGAAACAACATCTATT
TGCAATGAAATGATTAAAAATATGGAATCTGATTTGAAAAAAGAGTTTGC
AAAGGTTCTCAGTGAAAAATTGTCCGAACAGTATGATAATTTCGTCAAGT
TTAATAAGGATTGGATTTTACCTCCATTGGATTCAGACTGTTGGTACGTT
TGCTAAAAACGAATCGGTGATTTTGCATTTTGAATAAACTGTTGATTGTT
TTTAATATTAAACCAAATACATTAGCTTGGCTAGATCG
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protein sequence of SMED30013894-orf-1

>SMED30013894-orf-1 ID=SMED30013894-orf-1|Name=SMED30013894-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=169bp
MACSTLKRPCEIEDVENSNLRYMYPYSLKRHSDITEFTNPSKKMCLYSDH
QEKRSLYVSQSTIYHQLHTIEEVQIPNEKEEFDSDASSTSLCTETSKNQS
VFSENIPKYSLTETTSICNEMIKNMESDLKKEFAKVLSEKLSEQYDNFVK
FNKDWILPPLDSDCWYVC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR024132Akirin
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005654nucleoplasm
GO:0031965nuclear membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024132AkirinPANTHERPTHR13293UNCHARACTERIZEDcoord: 89..156
NoneNo IPR availablePANTHERPTHR13293:SF6AKIRIN-RELATEDcoord: 89..156