PWWP domain-containing protein

Overview
NamePWWP domain-containing protein
Smed IDSMED30013889
Length (bp)3590
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of PWWP domain-containing protein (SMED30013889) t-SNE clustered cells

Violin plots show distribution of expression levels for PWWP domain-containing protein (SMED30013889) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of PWWP domain-containing protein (SMED30013889) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for PWWP domain-containing protein (SMED30013889) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30013889

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30013889SMESG000026652.1 SmedASXL_016658SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30013889SMESG000026652.1 SmedASXL_016658SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30013889SMESG000026652.1 dd_Smed_v4_4650_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013889SMESG000026652.1 SMESG000026651.1 Contig35348newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013889SMESG000026652.1 SMESG000026651.1 Contig35348uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013889SMESG000026652.1 SMESG000026651.1 Contig39607newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013889SMESG000026652.1 SMESG000026651.1 Contig39607uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30013889SMESG000026652.1 dd_Smed_v4_4650_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of PWWP domain-containing protein vs. Ensembl Human
Match: HDGFL3 (HDGF like 3 [Source:HGNC Symbol;Acc:HGNC:24937])

HSP 1 Score: 96.6709 bits (239), Expect = 6.011e-22
Identity = 43/84 (51.19%), Postives = 57/84 (67.86%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDGN-YPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            ++K  D VFAK+   P+WPARI++LP+    P  N YPIFFFGTHE  FL  K L PY E K K+ ++++ + F E +WEIENN
Sbjct:    8 EYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENN 91          
BLAST of PWWP domain-containing protein vs. Ensembl Human
Match: PSIP1 (PC4 and SFRS1 interacting protein 1 [Source:HGNC Symbol;Acc:HGNC:9527])

HSP 1 Score: 98.5969 bits (244), Expect = 1.685e-21
Identity = 41/84 (48.81%), Postives = 58/84 (69.05%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D +FAK+   P+WPAR++++PD  +  P    PIFFFGTHE  FL  K + PY+ENK+KY + ++ + F E +WEI+NN
Sbjct:    4 DFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Human
Match: PSIP1 (PC4 and SFRS1 interacting protein 1 [Source:HGNC Symbol;Acc:HGNC:9527])

HSP 1 Score: 98.9821 bits (245), Expect = 1.702e-21
Identity = 41/84 (48.81%), Postives = 58/84 (69.05%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D +FAK+   P+WPAR++++PD  +  P    PIFFFGTHE  FL  K + PY+ENK+KY + ++ + F E +WEI+NN
Sbjct:    4 DFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Human
Match: PSIP1 (PC4 and SFRS1 interacting protein 1 [Source:HGNC Symbol;Acc:HGNC:9527])

HSP 1 Score: 98.9821 bits (245), Expect = 1.702e-21
Identity = 41/84 (48.81%), Postives = 58/84 (69.05%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D +FAK+   P+WPAR++++PD  +  P    PIFFFGTHE  FL  K + PY+ENK+KY + ++ + F E +WEI+NN
Sbjct:    4 DFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Human
Match: PSIP1 (PC4 and SFRS1 interacting protein 1 [Source:HGNC Symbol;Acc:HGNC:9527])

HSP 1 Score: 99.3673 bits (246), Expect = 8.469e-21
Identity = 41/84 (48.81%), Postives = 58/84 (69.05%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D +FAK+   P+WPAR++++PD  +  P    PIFFFGTHE  FL  K + PY+ENK+KY + ++ + F E +WEI+NN
Sbjct:    4 DFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Fly
Match: CG7946 (gene:FBgn0039743 transcript:FBtr0085561)

HSP 1 Score: 51.9878 bits (123), Expect = 2.682e-6
Identity = 30/83 (36.14%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   85 FKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNS--RYQHFTEAIWEIEN 327
            + I D VFAK+   P WPA+I K     S  +  Y ++F+GT E   +  + L PYA NK+++      +   F EAI +IE+
Sbjct:   10 YSIGDLVFAKVKGYPPWPAKITK-----SNNNKKYNVYFYGTGETANIKLEDLFPYASNKERFATEKIMKRAKFIEAIDQIES 87          
BLAST of PWWP domain-containing protein vs. Ensembl Fly
Match: CG7946 (gene:FBgn0039743 transcript:FBtr0310420)

HSP 1 Score: 51.9878 bits (123), Expect = 2.682e-6
Identity = 30/83 (36.14%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   85 FKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNS--RYQHFTEAIWEIEN 327
            + I D VFAK+   P WPA+I K     S  +  Y ++F+GT E   +  + L PYA NK+++      +   F EAI +IE+
Sbjct:   10 YSIGDLVFAKVKGYPPWPAKITK-----SNNNKKYNVYFYGTGETANIKLEDLFPYASNKERFATEKIMKRAKFIEAIDQIES 87          
BLAST of PWWP domain-containing protein vs. Ensembl Zebrafish
Match: psip1a (PC4 and SFRS1 interacting protein 1a [Source:ZFIN;Acc:ZDB-GENE-050522-104])

HSP 1 Score: 98.5969 bits (244), Expect = 3.791e-21
Identity = 42/85 (49.41%), Postives = 56/85 (65.88%), Query Frame = 1
Query:   79 EDFKINDRVFAKLPNCPYWPARINKLPDYISVPDG-NYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            +DFK  D +FAK+   P+WPARI+++PD    P    +PIFFFGTHE  FL  K + PY  NK KY + ++ + F E +WEIENN
Sbjct:    3 QDFKAGDLIFAKMKGYPHWPARIDEIPDGAVKPSNIKFPIFFFGTHETAFLGPKDIFPYLTNKDKYGKPNKRKGFNEGLWEIENN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Zebrafish
Match: hdgfl2 (HDGF like 2 [Source:NCBI gene;Acc:80965])

HSP 1 Score: 82.8037 bits (203), Expect = 1.162e-15
Identity = 36/83 (43.37%), Postives = 52/83 (62.65%), Query Frame = 1
Query:   85 FKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            F+  D VFAK+   P+WPARI  + D  +  P    PIFFFGTHE  FL  K L  Y +N++++ + ++ + F E +WEI+NN
Sbjct:    5 FRPGDLVFAKMKGYPHWPARIEDVADGAVKPPPNKIPIFFFGTHETAFLAPKDLFAYEKNQERFGKPNKRKGFNEGLWEIQNN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Zebrafish
Match: hdgfl2 (HDGF like 2 [Source:NCBI gene;Acc:80965])

HSP 1 Score: 82.8037 bits (203), Expect = 1.301e-15
Identity = 36/83 (43.37%), Postives = 52/83 (62.65%), Query Frame = 1
Query:   85 FKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            F+  D VFAK+   P+WPARI  + D  +  P    PIFFFGTHE  FL  K L  Y +N++++ + ++ + F E +WEI+NN
Sbjct:    5 FRPGDLVFAKMKGYPHWPARIEDVADGAVKPPPNKIPIFFFGTHETAFLAPKDLFAYEKNQERFGKPNKRKGFNEGLWEIQNN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Zebrafish
Match: hdgfl2 (HDGF like 2 [Source:NCBI gene;Acc:80965])

HSP 1 Score: 82.8037 bits (203), Expect = 1.301e-15
Identity = 36/83 (43.37%), Postives = 52/83 (62.65%), Query Frame = 1
Query:   85 FKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            F+  D VFAK+   P+WPARI  + D  +  P    PIFFFGTHE  FL  K L  Y +N++++ + ++ + F E +WEI+NN
Sbjct:    5 FRPGDLVFAKMKGYPHWPARIEDVADGAVKPPPNKIPIFFFGTHETAFLAPKDLFAYEKNQERFGKPNKRKGFNEGLWEIQNN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Zebrafish
Match: psip1b (PC4 and SFRS1 interacting protein 1b [Source:ZFIN;Acc:ZDB-GENE-030131-3273])

HSP 1 Score: 76.2554 bits (186), Expect = 1.489e-13
Identity = 38/85 (44.71%), Postives = 48/85 (56.47%), Query Frame = 1
Query:   76 IEDFKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
             +DF   D VFAK+   P+WPARI         P    PIFF+GTH   FL  K +V Y  NKQKY + ++   F E +WEIEN+
Sbjct:   13 TDDFTPGDVVFAKMKGYPFWPARIA----EGKAPKNKIPIFFYGTHSTTFLFPKDIVHYWPNKQKYAQANKRGGFEEGMWEIEND 93          
BLAST of PWWP domain-containing protein vs. Ensembl Xenopus
Match: hdgfl3 (HDGF like 3 [Source:NCBI gene;Acc:100125195])

HSP 1 Score: 96.2857 bits (238), Expect = 8.187e-22
Identity = 42/84 (50.00%), Postives = 58/84 (69.05%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDGN-YPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            D+K  + VFAK+   P+WPARI++LP+    P  N +PIFFFGTHE  FL  K L PY E K+K+ ++++ + F E +WEIENN
Sbjct:    8 DYKAGELVFAKMKGYPHWPARIDELPEGAVKPPANKFPIFFFGTHETAFLGPKDLFPYKEYKEKFGKSNKRKGFNEGLWEIENN 91          
BLAST of PWWP domain-containing protein vs. Ensembl Xenopus
Match: znf711 (zinc finger protein 711 [Source:Xenbase;Acc:XB-GENE-956382])

HSP 1 Score: 102.064 bits (253), Expect = 1.256e-21
Identity = 43/84 (51.19%), Postives = 57/84 (67.86%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D +FAK+   P+WPAR+++LPD  +  P    PIFFFGTHE  FL  K + PY ENK+KY + ++ + F E +WEIENN
Sbjct:    4 DFKPGDLIFAKMKGYPHWPARVDELPDGAVKPPTNKMPIFFFGTHETAFLGPKDIFPYDENKEKYAKPNKRKGFNEGLWEIENN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Xenopus
Match: znf711 (zinc finger protein 711 [Source:Xenbase;Acc:XB-GENE-956382])

HSP 1 Score: 102.064 bits (253), Expect = 1.311e-21
Identity = 43/84 (51.19%), Postives = 57/84 (67.86%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D +FAK+   P+WPAR+++LPD  +  P    PIFFFGTHE  FL  K + PY ENK+KY + ++ + F E +WEIENN
Sbjct:    4 DFKPGDLIFAKMKGYPHWPARVDELPDGAVKPPTNKMPIFFFGTHETAFLGPKDIFPYDENKEKYAKPNKRKGFNEGLWEIENN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Xenopus
Match: hdgfl3 (HDGF like 3 [Source:NCBI gene;Acc:100125195])

HSP 1 Score: 95.9005 bits (237), Expect = 1.464e-21
Identity = 42/84 (50.00%), Postives = 58/84 (69.05%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDGN-YPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            D+K  + VFAK+   P+WPARI++LP+    P  N +PIFFFGTHE  FL  K L PY E K+K+ ++++ + F E +WEIENN
Sbjct:    8 DYKAGELVFAKMKGYPHWPARIDELPEGAVKPPANKFPIFFFGTHETAFLGPKDLFPYKEYKEKFGKSNKRKGFNEGLWEIENN 91          
BLAST of PWWP domain-containing protein vs. Ensembl Xenopus
Match: znf711 (zinc finger protein 711 [Source:Xenbase;Acc:XB-GENE-956382])

HSP 1 Score: 102.064 bits (253), Expect = 1.726e-21
Identity = 43/84 (51.19%), Postives = 57/84 (67.86%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D +FAK+   P+WPAR+++LPD  +  P    PIFFFGTHE  FL  K + PY ENK+KY + ++ + F E +WEIENN
Sbjct:    4 DFKPGDLIFAKMKGYPHWPARVDELPDGAVKPPTNKMPIFFFGTHETAFLGPKDIFPYDENKEKYAKPNKRKGFNEGLWEIENN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Mouse
Match: Hdgfl3 (HDGF like 3 [Source:MGI Symbol;Acc:MGI:1352760])

HSP 1 Score: 98.5969 bits (244), Expect = 8.253e-23
Identity = 52/138 (37.68%), Postives = 75/138 (54.35%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDGN-YPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSADSNAEKVIDVENPEINDVEG 492
            ++K  D VFAK+   P+WPARI++LP+    P  N YPIFFFGTHE  FL  K L PY E K K+ ++++ + F E +WEIENN  +         +    E   TE      +D S++   ++V D +    N+  G
Sbjct:    8 EYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENNPGVKFTGYQTIQQQSSSE---TEGEGGNTADASSEEEGDRVEDGKGKRKNEKGG 142          
BLAST of PWWP domain-containing protein vs. Ensembl Mouse
Match: Hdgfl3 (HDGF like 3 [Source:MGI Symbol;Acc:MGI:1352760])

HSP 1 Score: 98.5969 bits (244), Expect = 1.131e-22
Identity = 52/138 (37.68%), Postives = 75/138 (54.35%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDGN-YPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSADSNAEKVIDVENPEINDVEG 492
            ++K  D VFAK+   P+WPARI++LP+    P  N YPIFFFGTHE  FL  K L PY E K K+ ++++ + F E +WEIENN  +         +    E   TE      +D S++   ++V D +    N+  G
Sbjct:    8 EYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENNPGVKFTGYQTIQQQSSSE---TEGEGGNTADASSEEEGDRVEDGKGKRKNEKGG 142          
BLAST of PWWP domain-containing protein vs. Ensembl Mouse
Match: Psip1 (PC4 and SFRS1 interacting protein 1 [Source:MGI Symbol;Acc:MGI:2142116])

HSP 1 Score: 92.0485 bits (227), Expect = 7.001e-22
Identity = 41/85 (48.24%), Postives = 58/85 (68.24%), Query Frame = 1
Query:   79 EDFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
             DFK  D +FAK+   P+WPAR++++PD  +  P    PIFFFGTHE  FL  K + PY+ENK+KY + ++ + F E +WEI+NN
Sbjct:    3 RDFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Mouse
Match: Psip1 (PC4 and SFRS1 interacting protein 1 [Source:MGI Symbol;Acc:MGI:2142116])

HSP 1 Score: 98.9821 bits (245), Expect = 1.011e-21
Identity = 41/84 (48.81%), Postives = 58/84 (69.05%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D +FAK+   P+WPAR++++PD  +  P    PIFFFGTHE  FL  K + PY+ENK+KY + ++ + F E +WEI+NN
Sbjct:    4 DFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Mouse
Match: Psip1 (PC4 and SFRS1 interacting protein 1 [Source:MGI Symbol;Acc:MGI:2142116])

HSP 1 Score: 98.9821 bits (245), Expect = 1.029e-21
Identity = 41/84 (48.81%), Postives = 58/84 (69.05%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D +FAK+   P+WPAR++++PD  +  P    PIFFFGTHE  FL  K + PY+ENK+KY + ++ + F E +WEI+NN
Sbjct:    4 DFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNN 87          
BLAST of PWWP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9JMG7|HDGR3_MOUSE (Hepatoma-derived growth factor-related protein 3 OS=Mus musculus OX=10090 GN=Hdgfl3 PE=1 SV=2)

HSP 1 Score: 98.5969 bits (244), Expect = 5.773e-22
Identity = 52/138 (37.68%), Postives = 75/138 (54.35%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDGN-YPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSADSNAEKVIDVENPEINDVEG 492
            ++K  D VFAK+   P+WPARI++LP+    P  N YPIFFFGTHE  FL  K L PY E K K+ ++++ + F E +WEIENN  +         +    E   TE      +D S++   ++V D +    N+  G
Sbjct:    8 EYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENNPGVKFTGYQTIQQQSSSE---TEGEGGNTADASSEEEGDRVEDGKGKRKNEKGG 142          
BLAST of PWWP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q923W4|HDGR3_RAT (Hepatoma-derived growth factor-related protein 3 OS=Rattus norvegicus OX=10116 GN=Hdgfl3 PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 5.941e-22
Identity = 52/138 (37.68%), Postives = 75/138 (54.35%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDGN-YPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSADSNAEKVIDVENPEINDVEG 492
            ++K  D VFAK+   P+WPARI++LP+    P  N YPIFFFGTHE  FL  K L PY E K K+ ++++ + F E +WEIENN  +         +    E   TE      +D S++   ++V D +    N+  G
Sbjct:    8 EYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENNPGVKFTGYQTIQQQSSSE---TEGEGGNTADASSEEEGDRVEDGKGKRKNEKGG 142          
BLAST of PWWP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9Y3E1|HDGR3_HUMAN (Hepatoma-derived growth factor-related protein 3 OS=Homo sapiens OX=9606 GN=HDGFL3 PE=1 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 2.887e-21
Identity = 43/84 (51.19%), Postives = 57/84 (67.86%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDGN-YPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            ++K  D VFAK+   P+WPARI++LP+    P  N YPIFFFGTHE  FL  K L PY E K K+ ++++ + F E +WEIENN
Sbjct:    8 EYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENN 91          
BLAST of PWWP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q66T72|PSIP1_FELCA (PC4 and SFRS1-interacting protein OS=Felis catus OX=9685 GN=PSIP1 PE=1 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 3.657e-20
Identity = 41/84 (48.81%), Postives = 58/84 (69.05%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D +FAK+   P+WPAR++++PD  +  P    PIFFFGTHE  FL  K + PY+ENK+KY + ++ + F E +WEI+NN
Sbjct:    4 DFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNN 87          
BLAST of PWWP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q812D1|PSIP1_RAT (PC4 and SFRS1-interacting protein OS=Rattus norvegicus OX=10116 GN=Psip1 PE=1 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 3.711e-20
Identity = 41/84 (48.81%), Postives = 58/84 (69.05%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D +FAK+   P+WPAR++++PD  +  P    PIFFFGTHE  FL  K + PY+ENK+KY + ++ + F E +WEI+NN
Sbjct:    4 DFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNN 87          
BLAST of PWWP domain-containing protein vs. TrEMBL
Match: A0A0R3U150 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS57 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 7.167e-22
Identity = 56/117 (47.86%), Postives = 75/117 (64.10%), Query Frame = 1
Query:   85 FKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSAD 435
            +K  DR+FAK+   P+WPARIN LP+ + VP G YPIFF+GT+EV FL  K + PY + K KY  N     F + + EIENN D+L   KD  AENFL + +D ++ T   +DP +D
Sbjct:    3 YKPCDRIFAKVKGHPHWPARINILPEDVKVPKGKYPIFFYGTYEVYFLAPKDIFPYEKWKHKYAVNKSRPTFQKGLHEIENNPDVLLYGKDANAENFLSQFYDFKRSTQ--NDPPSD 117          
BLAST of PWWP domain-containing protein vs. TrEMBL
Match: V9L7Q7 (Hepatoma-derived growth factor-related protein 3 OS=Callorhinchus milii OX=7868 PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 5.064e-21
Identity = 51/130 (39.23%), Postives = 74/130 (56.92%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDGN-YPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSADSNAEKVIDVEN 468
            ++K  D VFAK+   P+WPARI++LP+    P  N YPIFFFGTHE  FL  K L+PY E K K+ ++++ + F E +WEIENN  +  +      +    E   TE      +D S++    ++ID E 
Sbjct:    8 EYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLLPYKEYKDKFGKSNKRKGFNEGLWEIENNPGVKFMGYQAIQQQSSSE---TEGEGGNTADASSEGEGGEIIDEEG 134          
BLAST of PWWP domain-containing protein vs. TrEMBL
Match: A0A3S2PJ34 (PWWP domain-containing protein OS=Oryzias javanicus OX=123683 GN=OJAV_G00027240 PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 1.466e-20
Identity = 44/84 (52.38%), Postives = 59/84 (70.24%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDGN-YPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D VFAK+   P+WPARI++LPD    P  N +PIFFFGTHE  FL  K L+PY + K+K+ ++++ + F E +WEIENN
Sbjct:    8 DFKAGDLVFAKMKGYPHWPARIDELPDGAVKPPANKHPIFFFGTHETAFLGPKDLLPYGDYKEKFGKSNKRKGFNEGLWEIENN 91          
BLAST of PWWP domain-containing protein vs. TrEMBL
Match: A0A3P9HTD8 (HDGF like 3 OS=Oryzias latipes OX=8090 GN=HDGFL3 PE=4 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 1.843e-20
Identity = 43/84 (51.19%), Postives = 59/84 (70.24%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D VFAK+   P+WPARI++LPD  +  P   +PIFFFGTHE  FL  K L+PY + K+K+ ++++ + F E +WEIENN
Sbjct:    8 DFKAGDLVFAKMKGYPHWPARIDELPDGAVKPPSNKHPIFFFGTHETAFLGPKDLLPYGDYKEKFGKSNKRKGFNEGLWEIENN 91          
BLAST of PWWP domain-containing protein vs. TrEMBL
Match: A0A3B3H9C4 (HDGF like 3 OS=Oryzias latipes OX=8090 GN=HDGFL3 PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 2.062e-20
Identity = 43/84 (51.19%), Postives = 59/84 (70.24%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D VFAK+   P+WPARI++LPD  +  P   +PIFFFGTHE  FL  K L+PY + K+K+ ++++ + F E +WEIENN
Sbjct:    8 DFKAGDLVFAKMKGYPHWPARIDELPDGAVKPPSNKHPIFFFGTHETAFLGPKDLLPYGDYKEKFGKSNKRKGFNEGLWEIENN 91          
BLAST of PWWP domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000042912.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000680.1:36200:53477:-1 gene:ENSAMXG00000044093.1 transcript:ENSAMXT00000042912.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 95.5153 bits (236), Expect = 6.625e-22
Identity = 43/84 (51.19%), Postives = 58/84 (69.05%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDGN-YPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            ++K  D VFAK+   P+WPARI++LP+    P  N YPIFFFGTHE  FL  K L+PY E K K+ ++++ + F E +WEIENN
Sbjct:    8 EYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLLPYKEYKDKFGKSNKRKGFNEGLWEIENN 91          
BLAST of PWWP domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000035361.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000680.1:25149:53477:-1 gene:ENSAMXG00000044093.1 transcript:ENSAMXT00000035361.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 95.9005 bits (237), Expect = 8.664e-22
Identity = 43/84 (51.19%), Postives = 58/84 (69.05%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDGN-YPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            ++K  D VFAK+   P+WPARI++LP+    P  N YPIFFFGTHE  FL  K L+PY E K K+ ++++ + F E +WEIENN
Sbjct:    8 EYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLLPYKEYKDKFGKSNKRKGFNEGLWEIENN 91          
BLAST of PWWP domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000035815.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000680.1:36607:53562:-1 gene:ENSAMXG00000044093.1 transcript:ENSAMXT00000035815.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 91.6633 bits (226), Expect = 9.674e-22
Identity = 44/90 (48.89%), Postives = 60/90 (66.67%), Query Frame = 1
Query:   76 IEDFKINDRVFAKLPNCPYWPARINKLPDYISVPDGN-YPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDIL 342
            ++ F   D VFAK+   P+WPARI++LP+    P  N YPIFFFGTHE  FL  K L+PY E K K+ ++++ + F E +WEIENN  +L
Sbjct:    2 VQRFSSGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLLPYKEYKDKFGKSNKRKGFNEGLWEIENNPAVL 91          
BLAST of PWWP domain-containing protein vs. Ensembl Cavefish
Match: psip1a (PC4 and SFRS1-interacting protein-like [Source:NCBI gene;Acc:103040307])

HSP 1 Score: 93.9745 bits (232), Expect = 1.855e-19
Identity = 41/84 (48.81%), Postives = 55/84 (65.48%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDG-NYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            D+K  D +FAK+   P+WPARI+++PD    P    +PIFFFGTHE  FL  K + PY  NK KY + ++ + F E +WEIENN
Sbjct:    4 DYKPGDLIFAKMKGYPHWPARIDEVPDGAVKPANIKFPIFFFGTHETAFLGPKDIFPYLPNKDKYGKPNKRKGFNEGLWEIENN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Cavefish
Match: psip1a (PC4 and SFRS1-interacting protein-like [Source:NCBI gene;Acc:103040307])

HSP 1 Score: 94.3597 bits (233), Expect = 2.138e-19
Identity = 41/84 (48.81%), Postives = 55/84 (65.48%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDG-NYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            D+K  D +FAK+   P+WPARI+++PD    P    +PIFFFGTHE  FL  K + PY  NK KY + ++ + F E +WEIENN
Sbjct:    4 DYKPGDLIFAKMKGYPHWPARIDEVPDGAVKPANIKFPIFFFGTHETAFLGPKDIFPYLPNKDKYGKPNKRKGFNEGLWEIENN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010402.1 (pep scaffold:Pmarinus_7.0:GL477087:153717:159376:-1 gene:ENSPMAG00000009421.1 transcript:ENSPMAT00000010402.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 87.4261 bits (215), Expect = 1.114e-19
Identity = 37/84 (44.05%), Postives = 55/84 (65.48%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            ++K  D +FAK+   P+WPARI+++ +  +  P   YPIFF+GTHE  FL  K + PY + K KY + ++ + F E +WEIENN
Sbjct:    4 EYKPGDLIFAKMKGYPHWPARIDEVAEGAVKPPSSKYPIFFYGTHETAFLGVKDIFPYLQTKNKYGKPNKRKGFNEGLWEIENN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001585.1 (pep scaffold:Pmarinus_7.0:GL478812:497860:498661:1 gene:ENSPMAG00000001433.1 transcript:ENSPMAT00000001585.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 47.3654 bits (111), Expect = 1.352e-6
Identity = 23/80 (28.75%), Postives = 39/80 (48.75%), Query Frame = 1
Query:   91 INDRVFAKLPNCPYWPARINKLPDYISVPDG-----NYPIFFFGTHEVEFLPSKSLVPYAEN-KQKYIRNSRYQHFTEAI 312
            + D V+AKL  CP+WPA++  +   +    G        + +FG+    FLP    VP+ EN + +Y R  +   +  A+
Sbjct:   26 VGDIVWAKLHGCPWWPAQVRSVSVSVQEESGLVLSQEAKMAWFGSTTTSFLPLSQAVPFMENFEARYSRRKKSNGYRRAV 105          
BLAST of PWWP domain-containing protein vs. Ensembl Nematostella
Match: EDO42006 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2I6])

HSP 1 Score: 66.2402 bits (160), Expect = 2.163e-13
Identity = 31/82 (37.80%), Postives = 48/82 (58.54%), Query Frame = 1
Query:   85 FKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            ++  D ++AK+   P+WPARI+       +P   YPIFF+GTHE   +  K L PY ++K K+ +  + + F EA+ EI  N
Sbjct:    3 YQPGDLIWAKMRGYPHWPARIDLAAKDEVIPAKKYPIFFYGTHETAVMLPKDLFPYEKHKHKFAKPCKRKGFMEALEEIVKN 84          
BLAST of PWWP domain-containing protein vs. Ensembl Medaka
Match: HDGFL3 (hepatoma-derived growth factor-related protein 3 [Source:NCBI gene;Acc:101175133])

HSP 1 Score: 101.679 bits (252), Expect = 7.761e-24
Identity = 43/84 (51.19%), Postives = 59/84 (70.24%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            DFK  D VFAK+   P+WPARI++LPD  +  P   +PIFFFGTHE  FL  K L+PY + K+K+ ++++ + F E +WEIENN
Sbjct:    8 DFKAGDLVFAKMKGYPHWPARIDELPDGAVKPPSNKHPIFFFGTHETAFLGPKDLLPYGDYKEKFGKSNKRKGFNEGLWEIENN 91          
BLAST of PWWP domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000046328.1 (heparin binding growth factor [Source:NCBI gene;Acc:101161288])

HSP 1 Score: 96.6709 bits (239), Expect = 6.349e-22
Identity = 41/89 (46.07%), Postives = 60/89 (67.42%), Query Frame = 1
Query:   67 SFKIEDFKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            S + +++K  D VFAK+   P+WPARI++LP+  +  P   Y +FFFGTHE  FL +K L PY E K+K+ + ++ + F E +WEIENN
Sbjct:    4 SNRQKEYKPGDLVFAKMKGYPHWPARIDELPEGAVKSPSNKYQVFFFGTHETAFLGAKDLFPYEECKEKFGKANKRKGFAEGLWEIENN 92          
BLAST of PWWP domain-containing protein vs. Ensembl Medaka
Match: HDGFL3 (hepatoma-derived growth factor-related protein 3 [Source:NCBI gene;Acc:101175133])

HSP 1 Score: 93.2041 bits (230), Expect = 1.603e-21
Identity = 42/83 (50.60%), Postives = 58/83 (69.88%), Query Frame = 1
Query:   85 FKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            FK  D VFAK+   P+WPARI++LPD  +  P   +PIFFFGTHE  FL  K L+PY + K+K+ ++++ + F E +WEIENN
Sbjct:    3 FKAGDLVFAKMKGYPHWPARIDELPDGAVKPPSNKHPIFFFGTHETAFLGPKDLLPYGDYKEKFGKSNKRKGFNEGLWEIENN 85          
BLAST of PWWP domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000025330.2 (pep primary_assembly:ASM223467v1:1:16538339:16540560:1 gene:ENSORLG00000020443.2 transcript:ENSORLT00000025330.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 88.9669 bits (219), Expect = 1.093e-20
Identity = 42/84 (50.00%), Postives = 56/84 (66.67%), Query Frame = 1
Query:   82 DFKINDRVFAKLPNCPYWPARINKLPDYISVPDG-NYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            D+K  D +FAK+   P+WPARI+++PD    P    YPIFFFGTHE  FL  K + PY  NK+KY + ++ + F E +WEIENN
Sbjct:    4 DWKPGDLIFAKMKGYPHWPARIDEVPDGAVKPSNIKYPIFFFGTHETAFLGPKDIFPYLLNKEKYAKPNKRKGFNEGLWEIENN 87          
BLAST of PWWP domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000021204.3 (HDGF like 2 [Source:NCBI gene;Acc:101158434])

HSP 1 Score: 84.3445 bits (207), Expect = 3.592e-16
Identity = 37/83 (44.58%), Postives = 52/83 (62.65%), Query Frame = 1
Query:   85 FKINDRVFAKLPNCPYWPARINKLPD-YISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENN 330
            F   D VFAK+   P+WPARI  + D  +  P    PIFFFGTHE  FL +K L PY + +++Y + ++ + F E +WEI+NN
Sbjct:    5 FMPGDLVFAKMKGYPHWPARIEDVADGAVKPPPNKIPIFFFGTHETAFLAAKDLFPYEKYRERYGKPNKRKGFNEGLWEIQNN 87          
BLAST of PWWP domain-containing protein vs. Planmine SMEST
Match: SMESG000026652.1 (SMESG000026652.1)

HSP 1 Score: 2083.92 bits (5398), Expect = 0.000e+0
Identity = 1117/1119 (99.82%), Postives = 1118/1119 (99.91%), Query Frame = 1
Query:   61 YFSFKIEDFKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSADSNAEKVIDVENPEINDVEGIMNLGENEWNEIFTIEEQEIHNNLSQLADPVFGNSVSDAANNTATPNGPVKTAIIREAISITPTKIKICQTSVPVSNENHEAQSSLHRSALHNNCKVIVEKLSIIQINRVIQSNFSVGLMEDLSKKNQKKPPSFVSSKPALTPTPANIDPKPKKAAISVNDSNPISTKXXXXXXXXXXXXXXXXDPRKPATSNKILSTINKNXXXXXXXXXXXXXXXNQSCVIVSPPKIKSSSELKRKQEKIENELKILPTIKLKLVRPNPDTADKNDKLPIXXXXXXXXXXXXXNSDSRRSGKIKTKERKICKTDYMATSRNTNDKPLTFDNLFNKKFSILGSGLXXXXXXXXDVVKEDPESSGVGAVTANQQQRQQDTEENKPIKQNPFETEEDEGNFWPKMKECEDEFNTQPKHNGGYEETGGDFVXXXXXXAPWPVARTEEDMEPNRNCIKHEMKEGSNDKDVGTEEEEMSSKWYTNGTGAEEPRGESEIDPENADKKDPWSNYMAKKQKEDNLVGENVVELPDSDSEMFDVILKEEPGNPVKRSAGNVWSHIQEYQSQFDNGKQDVMDTMDGVEIGPDDAIRNMEYIQDAKWLNSTNEDFLDSPPLPQAMPDEENVSLCQNVDLASTIVHKKWYDRNRLPSPPNNVPSLMDIDTSRAMKEFRGPESWVDSGTAFGRAFRESSHNNRARNRHMSTNAKSRNNFVSPNSKTAAFQNIPFEQLFPPFKAEYFTNNGTKGNNNHQLSTPQPSEQQPGKAQNRAGKTQNLRPTDCRMPGNKLPGMMPSGDPYQYYGDPYHHYAHGMAMVEDPXXXXXXXXXXXXXXXHLLQQPSPMTSRVARPHSSAPVPITPESPDPYTDDAADPLTVGSKPSTIEPHKLIDPVAAASNKKXXXXXXXXXXXXFSYMTENGYYLPSEEEMFAYFYSCYDGHPPTAAAFGPMNPYHQPNVPARPYQPYSTANDPITYTPASPDPEEMCPANSNNP 3417
            + SFKIEDFKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSADSNAEKVIDVENPEINDVEGIMNLGENEWNEIFTIEEQEIHNNLSQLADPVFGNSVSDAANNTATPNGPVKTAIIREAISITPTKIKICQTSVPVSNENHEAQSSLHRSALHNNCKVIVEKLSIIQINRVIQSNFSVGLMEDLSKKNQKKPPSFVSSKPALTPTPANIDPKPKKAAISVNDSNPISTKPQIQPATTIAATAIAPDPRKPATSNKILSTINKNSVSRIIDAISIDSDDNQSCVIVSPPKIKSSSELKRKQEKIENELKILPTIKLKLVRPNPDTADKNDKLPITGVSGGTGATAAGNSDSRRSGKIKTKERKICKTDYMATSRNTNDKPLTFDNLFNKKFSILGSGLKIAKKAIADVVKEDPESSGVGAVTANQQQRQQDTEENKPIKQNPFETEEDEGNFWPKMKECEDEFNTQPKHNGGYEETGGDFVDDDDDDAPWPVARTEEDMEPNRNCIKHEMKEGSNDKDVGTEEEEMSSKWYTNGTGAEEPRGESEIDPENADKKDPWSNYMAKKQKEDNLVGENVVELPDSDSEMFDVILKEEPGNPVKRSAGNVWSHIQEYQSQFDNGKQDVMDTMDGVEIGPDDAIRNMEYIQDAKWLNSTNEDFLDSPPLPQAMPDEENVSLCQNVDLASTIVHKKWYDRNRLPSPPNNVPSLMDIDTSRAMKEFRGPESWVDSGTAFGRAFRESSHNNRARNRHMSTNAKSRNNFVSPNSKTAAFQNIPFEQLFPPFKAEYFTNNGTKGNNNHQLSTPQPSEQQPGKAQNRAGKTQNLRPTDCRMPGNKLPGMMPSGDPYQYYGDPYHHYAHGMAMVEDPYYYYYYNNYYYGNNNHLLQQPSPMTSRVARPHSSAPVPITPESPDPYTDDAADPLTVGSKPSTIEPHKLIDPVAAASNKKPPPMSQMYYPQQFSYMTENGYYLPSEEEMFAYFYSCYDGHPPTAAAFGPMNPYHQPNVPARPYQPYSTANDPITYTPASPDPEEMCPANSNNP
Sbjct:    5 FQSFKIEDFKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSADSNAEKVIDVENPEINDVEGIMNLGENEWNEIFTIEEQEIHNNLSQLADPVFGNSVSDAANNTATPNGPVKTAIIREAISITPTKIKICQTSVPVSNENHEAQSSLHRSALHNNCKVIVEKLSIIQINRVIQSNFSVGLMEDLSKKNQKKPPSFVSSKPALTPTPANIDPKPKKAAISVNDSNPISTKPQIQPATTIAATAIAPDPRKPATSNKILSTINKNSVSRIIDAISIDSDDNQSCVIVSPPKIKSSSELKRKQEKIENELKILPTIKLKLVRPNPDTADKNDKLPITGVSGGTGATAAGNSDSRRSGKIKTKERKICKTDYMATSRNTNDKPLTFDNLFNKKFSILGSGLKIAKKAIADVVKEDPESSGVGAVTANQQQRQQDTEENKPIKQNPFETEEDEGNFWPKMKECEDEFNTQPKHNGGYEETGGDFVDDDDDDAPWPVARTEEDMEPNRNCIKHEMKEGSNDKDVGTEEEEMSSKWYTNGTGAEEPRGESEIDPENADKKDPWSNYMAKKQKEDNLVGENVVELPDSDSEMFDVILKEEPGNPVKRSAGNVWSHIQEYQSQFDNGKQDVMDTMDGVEIGPDDAIRNMEYIQDAKWLNSTNEDFLDSPPLPQAMPDEENVSLCQNVDLASTIVHKKWYDRNRLPSPPNNVPSLMDIDTSRAMKEFRGPESWVDSGTAFGRAFRESSHNNRARNRHMSTNAKSRNNFVSPNSKTAAFQNIPFEQLFPPFKAEYFTNNGTKGNNNHQLSTPQPSEQQPGKAQNRAGKTQNLRPTDCRMPGNKLPGMMPSGDPYQYYGDPYHHYAHGMAMVEDPYYYYYYNNYYYGNNNHLLQQPSPMTSRVARPHSSAPVPITPESPDPYTDDAADPLTVGSKPSTIEPHKLIDPVAAASNKKPPPMSQMYYPQQFSYMTENGYYLPSEEEMFAYFYSCYDGHPPTAAAFGPMNPYHQPNVPARPYQPYSTANDPITYTPASPDPEEMCPANSNNP 1123          
BLAST of PWWP domain-containing protein vs. Planmine SMEST
Match: SMESG000026652.1 (SMESG000026652.1)

HSP 1 Score: 2080.45 bits (5389), Expect = 0.000e+0
Identity = 1116/1116 (100.00%), Postives = 1116/1116 (100.00%), Query Frame = 1
Query:   70 FKIEDFKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSADSNAEKVIDVENPEINDVEGIMNLGENEWNEIFTIEEQEIHNNLSQLADPVFGNSVSDAANNTATPNGPVKTAIIREAISITPTKIKICQTSVPVSNENHEAQSSLHRSALHNNCKVIVEKLSIIQINRVIQSNFSVGLMEDLSKKNQKKPPSFVSSKPALTPTPANIDPKPKKAAISVNDSNPISTKXXXXXXXXXXXXXXXXDPRKPATSNKILSTINKNXXXXXXXXXXXXXXXNQSCVIVSPPKIKSSSELKRKQEKIENELKILPTIKLKLVRPNPDTADKNDKLPIXXXXXXXXXXXXXNSDSRRSGKIKTKERKICKTDYMATSRNTNDKPLTFDNLFNKKFSILGSGLXXXXXXXXDVVKEDPESSGVGAVTANQQQRQQDTEENKPIKQNPFETEEDEGNFWPKMKECEDEFNTQPKHNGGYEETGGDFVXXXXXXAPWPVARTEEDMEPNRNCIKHEMKEGSNDKDVGTEEEEMSSKWYTNGTGAEEPRGESEIDPENADKKDPWSNYMAKKQKEDNLVGENVVELPDSDSEMFDVILKEEPGNPVKRSAGNVWSHIQEYQSQFDNGKQDVMDTMDGVEIGPDDAIRNMEYIQDAKWLNSTNEDFLDSPPLPQAMPDEENVSLCQNVDLASTIVHKKWYDRNRLPSPPNNVPSLMDIDTSRAMKEFRGPESWVDSGTAFGRAFRESSHNNRARNRHMSTNAKSRNNFVSPNSKTAAFQNIPFEQLFPPFKAEYFTNNGTKGNNNHQLSTPQPSEQQPGKAQNRAGKTQNLRPTDCRMPGNKLPGMMPSGDPYQYYGDPYHHYAHGMAMVEDPXXXXXXXXXXXXXXXHLLQQPSPMTSRVARPHSSAPVPITPESPDPYTDDAADPLTVGSKPSTIEPHKLIDPVAAASNKKXXXXXXXXXXXXFSYMTENGYYLPSEEEMFAYFYSCYDGHPPTAAAFGPMNPYHQPNVPARPYQPYSTANDPITYTPASPDPEEMCPANSNNP 3417
            FKIEDFKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSADSNAEKVIDVENPEINDVEGIMNLGENEWNEIFTIEEQEIHNNLSQLADPVFGNSVSDAANNTATPNGPVKTAIIREAISITPTKIKICQTSVPVSNENHEAQSSLHRSALHNNCKVIVEKLSIIQINRVIQSNFSVGLMEDLSKKNQKKPPSFVSSKPALTPTPANIDPKPKKAAISVNDSNPISTKPQIQPATTIAATAIAPDPRKPATSNKILSTINKNSVSRIIDAISIDSDDNQSCVIVSPPKIKSSSELKRKQEKIENELKILPTIKLKLVRPNPDTADKNDKLPITGVSGGTGATAAGNSDSRRSGKIKTKERKICKTDYMATSRNTNDKPLTFDNLFNKKFSILGSGLKIAKKAIADVVKEDPESSGVGAVTANQQQRQQDTEENKPIKQNPFETEEDEGNFWPKMKECEDEFNTQPKHNGGYEETGGDFVDDDDDDAPWPVARTEEDMEPNRNCIKHEMKEGSNDKDVGTEEEEMSSKWYTNGTGAEEPRGESEIDPENADKKDPWSNYMAKKQKEDNLVGENVVELPDSDSEMFDVILKEEPGNPVKRSAGNVWSHIQEYQSQFDNGKQDVMDTMDGVEIGPDDAIRNMEYIQDAKWLNSTNEDFLDSPPLPQAMPDEENVSLCQNVDLASTIVHKKWYDRNRLPSPPNNVPSLMDIDTSRAMKEFRGPESWVDSGTAFGRAFRESSHNNRARNRHMSTNAKSRNNFVSPNSKTAAFQNIPFEQLFPPFKAEYFTNNGTKGNNNHQLSTPQPSEQQPGKAQNRAGKTQNLRPTDCRMPGNKLPGMMPSGDPYQYYGDPYHHYAHGMAMVEDPYYYYYYNNYYYGNNNHLLQQPSPMTSRVARPHSSAPVPITPESPDPYTDDAADPLTVGSKPSTIEPHKLIDPVAAASNKKPPPMSQMYYPQQFSYMTENGYYLPSEEEMFAYFYSCYDGHPPTAAAFGPMNPYHQPNVPARPYQPYSTANDPITYTPASPDPEEMCPANSNNP
Sbjct:   11 FKIEDFKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSADSNAEKVIDVENPEINDVEGIMNLGENEWNEIFTIEEQEIHNNLSQLADPVFGNSVSDAANNTATPNGPVKTAIIREAISITPTKIKICQTSVPVSNENHEAQSSLHRSALHNNCKVIVEKLSIIQINRVIQSNFSVGLMEDLSKKNQKKPPSFVSSKPALTPTPANIDPKPKKAAISVNDSNPISTKPQIQPATTIAATAIAPDPRKPATSNKILSTINKNSVSRIIDAISIDSDDNQSCVIVSPPKIKSSSELKRKQEKIENELKILPTIKLKLVRPNPDTADKNDKLPITGVSGGTGATAAGNSDSRRSGKIKTKERKICKTDYMATSRNTNDKPLTFDNLFNKKFSILGSGLKIAKKAIADVVKEDPESSGVGAVTANQQQRQQDTEENKPIKQNPFETEEDEGNFWPKMKECEDEFNTQPKHNGGYEETGGDFVDDDDDDAPWPVARTEEDMEPNRNCIKHEMKEGSNDKDVGTEEEEMSSKWYTNGTGAEEPRGESEIDPENADKKDPWSNYMAKKQKEDNLVGENVVELPDSDSEMFDVILKEEPGNPVKRSAGNVWSHIQEYQSQFDNGKQDVMDTMDGVEIGPDDAIRNMEYIQDAKWLNSTNEDFLDSPPLPQAMPDEENVSLCQNVDLASTIVHKKWYDRNRLPSPPNNVPSLMDIDTSRAMKEFRGPESWVDSGTAFGRAFRESSHNNRARNRHMSTNAKSRNNFVSPNSKTAAFQNIPFEQLFPPFKAEYFTNNGTKGNNNHQLSTPQPSEQQPGKAQNRAGKTQNLRPTDCRMPGNKLPGMMPSGDPYQYYGDPYHHYAHGMAMVEDPYYYYYYNNYYYGNNNHLLQQPSPMTSRVARPHSSAPVPITPESPDPYTDDAADPLTVGSKPSTIEPHKLIDPVAAASNKKPPPMSQMYYPQQFSYMTENGYYLPSEEEMFAYFYSCYDGHPPTAAAFGPMNPYHQPNVPARPYQPYSTANDPITYTPASPDPEEMCPANSNNP 1126          
BLAST of PWWP domain-containing protein vs. Planmine SMEST
Match: SMESG000026652.1 (SMESG000026652.1)

HSP 1 Score: 2078.14 bits (5383), Expect = 0.000e+0
Identity = 1116/1116 (100.00%), Postives = 1116/1116 (100.00%), Query Frame = 1
Query:   70 FKIEDFKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSADSNAEKVIDVENPEINDVEGIMNLGENEWNEIFTIEEQEIHNNLSQLADPVFGNSVSDAANNTATPNGPVKTAIIREAISITPTKIKICQTSVPVSNENHEAQSSLHRSALHNNCKVIVEKLSIIQINRVIQSNFSVGLMEDLSKKNQKKPPSFVSSKPALTPTPANIDPKPKKAAISVNDSNPISTKXXXXXXXXXXXXXXXXDPRKPATSNKILSTINKNXXXXXXXXXXXXXXXNQSCVIVSPPKIKSSSELKRKQEKIENELKILPTIKLKLVRPNPDTADKNDKLPIXXXXXXXXXXXXXNSDSRRSGKIKTKERKICKTDYMATSRNTNDKPLTFDNLFNKKFSILGSGLXXXXXXXXDVVKEDPESSGVGAVTANQQQRQQDTEENKPIKQNPFETEEDEGNFWPKMKECEDEFNTQPKHNGGYEETGGDFVXXXXXXAPWPVARTEEDMEPNRNCIKHEMKEGSNDKDVGTEEEEMSSKWYTNGTGAEEPRGESEIDPENADKKDPWSNYMAKKQKEDNLVGENVVELPDSDSEMFDVILKEEPGNPVKRSAGNVWSHIQEYQSQFDNGKQDVMDTMDGVEIGPDDAIRNMEYIQDAKWLNSTNEDFLDSPPLPQAMPDEENVSLCQNVDLASTIVHKKWYDRNRLPSPPNNVPSLMDIDTSRAMKEFRGPESWVDSGTAFGRAFRESSHNNRARNRHMSTNAKSRNNFVSPNSKTAAFQNIPFEQLFPPFKAEYFTNNGTKGNNNHQLSTPQPSEQQPGKAQNRAGKTQNLRPTDCRMPGNKLPGMMPSGDPYQYYGDPYHHYAHGMAMVEDPXXXXXXXXXXXXXXXHLLQQPSPMTSRVARPHSSAPVPITPESPDPYTDDAADPLTVGSKPSTIEPHKLIDPVAAASNKKXXXXXXXXXXXXFSYMTENGYYLPSEEEMFAYFYSCYDGHPPTAAAFGPMNPYHQPNVPARPYQPYSTANDPITYTPASPDPEEMCPANSNNP 3417
            FKIEDFKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSADSNAEKVIDVENPEINDVEGIMNLGENEWNEIFTIEEQEIHNNLSQLADPVFGNSVSDAANNTATPNGPVKTAIIREAISITPTKIKICQTSVPVSNENHEAQSSLHRSALHNNCKVIVEKLSIIQINRVIQSNFSVGLMEDLSKKNQKKPPSFVSSKPALTPTPANIDPKPKKAAISVNDSNPISTKPQIQPATTIAATAIAPDPRKPATSNKILSTINKNSVSRIIDAISIDSDDNQSCVIVSPPKIKSSSELKRKQEKIENELKILPTIKLKLVRPNPDTADKNDKLPITGVSGGTGATAAGNSDSRRSGKIKTKERKICKTDYMATSRNTNDKPLTFDNLFNKKFSILGSGLKIAKKAIADVVKEDPESSGVGAVTANQQQRQQDTEENKPIKQNPFETEEDEGNFWPKMKECEDEFNTQPKHNGGYEETGGDFVDDDDDDAPWPVARTEEDMEPNRNCIKHEMKEGSNDKDVGTEEEEMSSKWYTNGTGAEEPRGESEIDPENADKKDPWSNYMAKKQKEDNLVGENVVELPDSDSEMFDVILKEEPGNPVKRSAGNVWSHIQEYQSQFDNGKQDVMDTMDGVEIGPDDAIRNMEYIQDAKWLNSTNEDFLDSPPLPQAMPDEENVSLCQNVDLASTIVHKKWYDRNRLPSPPNNVPSLMDIDTSRAMKEFRGPESWVDSGTAFGRAFRESSHNNRARNRHMSTNAKSRNNFVSPNSKTAAFQNIPFEQLFPPFKAEYFTNNGTKGNNNHQLSTPQPSEQQPGKAQNRAGKTQNLRPTDCRMPGNKLPGMMPSGDPYQYYGDPYHHYAHGMAMVEDPYYYYYYNNYYYGNNNHLLQQPSPMTSRVARPHSSAPVPITPESPDPYTDDAADPLTVGSKPSTIEPHKLIDPVAAASNKKPPPMSQMYYPQQFSYMTENGYYLPSEEEMFAYFYSCYDGHPPTAAAFGPMNPYHQPNVPARPYQPYSTANDPITYTPASPDPEEMCPANSNNP
Sbjct:   19 FKIEDFKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDPSADSNAEKVIDVENPEINDVEGIMNLGENEWNEIFTIEEQEIHNNLSQLADPVFGNSVSDAANNTATPNGPVKTAIIREAISITPTKIKICQTSVPVSNENHEAQSSLHRSALHNNCKVIVEKLSIIQINRVIQSNFSVGLMEDLSKKNQKKPPSFVSSKPALTPTPANIDPKPKKAAISVNDSNPISTKPQIQPATTIAATAIAPDPRKPATSNKILSTINKNSVSRIIDAISIDSDDNQSCVIVSPPKIKSSSELKRKQEKIENELKILPTIKLKLVRPNPDTADKNDKLPITGVSGGTGATAAGNSDSRRSGKIKTKERKICKTDYMATSRNTNDKPLTFDNLFNKKFSILGSGLKIAKKAIADVVKEDPESSGVGAVTANQQQRQQDTEENKPIKQNPFETEEDEGNFWPKMKECEDEFNTQPKHNGGYEETGGDFVDDDDDDAPWPVARTEEDMEPNRNCIKHEMKEGSNDKDVGTEEEEMSSKWYTNGTGAEEPRGESEIDPENADKKDPWSNYMAKKQKEDNLVGENVVELPDSDSEMFDVILKEEPGNPVKRSAGNVWSHIQEYQSQFDNGKQDVMDTMDGVEIGPDDAIRNMEYIQDAKWLNSTNEDFLDSPPLPQAMPDEENVSLCQNVDLASTIVHKKWYDRNRLPSPPNNVPSLMDIDTSRAMKEFRGPESWVDSGTAFGRAFRESSHNNRARNRHMSTNAKSRNNFVSPNSKTAAFQNIPFEQLFPPFKAEYFTNNGTKGNNNHQLSTPQPSEQQPGKAQNRAGKTQNLRPTDCRMPGNKLPGMMPSGDPYQYYGDPYHHYAHGMAMVEDPYYYYYYNNYYYGNNNHLLQQPSPMTSRVARPHSSAPVPITPESPDPYTDDAADPLTVGSKPSTIEPHKLIDPVAAASNKKPPPMSQMYYPQQFSYMTENGYYLPSEEEMFAYFYSCYDGHPPTAAAFGPMNPYHQPNVPARPYQPYSTANDPITYTPASPDPEEMCPANSNNP 1134          
BLAST of PWWP domain-containing protein vs. Planmine SMEST
Match: SMESG000069102.1 (SMESG000069102.1)

HSP 1 Score: 91.6633 bits (226), Expect = 5.644e-20
Identity = 44/107 (41.12%), Postives = 60/107 (56.07%), Query Frame = 1
Query:   85 FKINDRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAENKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKP 405
            F   ++ FAK+   P+WPARIN LP+ + +P G YPIFF+GTHEV FL S ++ PY + K K+        F   + E+ENN  I     DP A  FL + +    P
Sbjct:    3 FASGEKCFAKVKGFPHWPARINCLPEDVKIPKGKYPIFFYGTHEVCFLSSNAIFPYEKYKSKFSVARNTVGFNNGLEELENNSTIYQYGIDPKAVKFLSQFYTFSVP 109          
BLAST of PWWP domain-containing protein vs. Planmine SMEST
Match: SMESG000057564.1 (SMESG000057564.1)

HSP 1 Score: 90.5077 bits (223), Expect = 5.167e-18
Identity = 45/111 (40.54%), Postives = 65/111 (58.56%), Query Frame = 1
Query:   97 DRVFAKLPNCPYWPARINKLPDYISVPDGNYPIFFFGTHEVEFLPSKSLVPYAE-NKQKYIRNSRYQHFTEAIWEIENNFDILALDKDPAAENFLCEMHDTEKPTAKISDP 426
            D++FAK+    YWPAR+N LP  I +P    PIFF+GTHEV F+   S  PY E NK K+ +     HF + + EIEN   I     DP+AE+FL + ++ ++   + + P
Sbjct:   11 DKIFAKVKGFVYWPARVNPLPVDIVIPPNKIPIFFYGTHEVFFISPTSCWPYDEKNKAKWGKPKSRAHFNQGMDEIENQSTIYQYGADPSAEDFLAQFYEFKRSNGQHTTP 121          
The following BLAST results are available for this feature:
BLAST of PWWP domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
HDGFL36.011e-2251.19HDGF like 3 [Source:HGNC Symbol;Acc:HGNC:24937][more]
PSIP11.685e-2148.81PC4 and SFRS1 interacting protein 1 [Source:HGNC S... [more]
PSIP11.702e-2148.81PC4 and SFRS1 interacting protein 1 [Source:HGNC S... [more]
PSIP11.702e-2148.81PC4 and SFRS1 interacting protein 1 [Source:HGNC S... [more]
PSIP18.469e-2148.81PC4 and SFRS1 interacting protein 1 [Source:HGNC S... [more]
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BLAST of PWWP domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PWWP domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG79462.682e-636.14gene:FBgn0039743 transcript:FBtr0085561[more]
CG79462.682e-636.14gene:FBgn0039743 transcript:FBtr0310420[more]
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BLAST of PWWP domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
psip1a3.791e-2149.41PC4 and SFRS1 interacting protein 1a [Source:ZFIN;... [more]
hdgfl21.162e-1543.37HDGF like 2 [Source:NCBI gene;Acc:80965][more]
hdgfl21.301e-1543.37HDGF like 2 [Source:NCBI gene;Acc:80965][more]
hdgfl21.301e-1543.37HDGF like 2 [Source:NCBI gene;Acc:80965][more]
psip1b1.489e-1344.71PC4 and SFRS1 interacting protein 1b [Source:ZFIN;... [more]
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BLAST of PWWP domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hdgfl38.187e-2250.00HDGF like 3 [Source:NCBI gene;Acc:100125195][more]
znf7111.256e-2151.19zinc finger protein 711 [Source:Xenbase;Acc:XB-GEN... [more]
znf7111.311e-2151.19zinc finger protein 711 [Source:Xenbase;Acc:XB-GEN... [more]
hdgfl31.464e-2150.00HDGF like 3 [Source:NCBI gene;Acc:100125195][more]
znf7111.726e-2151.19zinc finger protein 711 [Source:Xenbase;Acc:XB-GEN... [more]
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BLAST of PWWP domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Hdgfl38.253e-2337.68HDGF like 3 [Source:MGI Symbol;Acc:MGI:1352760][more]
Hdgfl31.131e-2237.68HDGF like 3 [Source:MGI Symbol;Acc:MGI:1352760][more]
Psip17.001e-2248.24PC4 and SFRS1 interacting protein 1 [Source:MGI Sy... [more]
Psip11.011e-2148.81PC4 and SFRS1 interacting protein 1 [Source:MGI Sy... [more]
Psip11.029e-2148.81PC4 and SFRS1 interacting protein 1 [Source:MGI Sy... [more]
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BLAST of PWWP domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9JMG7|HDGR3_MOUSE5.773e-2237.68Hepatoma-derived growth factor-related protein 3 O... [more]
sp|Q923W4|HDGR3_RAT5.941e-2237.68Hepatoma-derived growth factor-related protein 3 O... [more]
sp|Q9Y3E1|HDGR3_HUMAN2.887e-2151.19Hepatoma-derived growth factor-related protein 3 O... [more]
sp|Q66T72|PSIP1_FELCA3.657e-2048.81PC4 and SFRS1-interacting protein OS=Felis catus O... [more]
sp|Q812D1|PSIP1_RAT3.711e-2048.81PC4 and SFRS1-interacting protein OS=Rattus norveg... [more]
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BLAST of PWWP domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3U1507.167e-2247.86Uncharacterized protein OS=Mesocestoides corti OX=... [more]
V9L7Q75.064e-2139.23Hepatoma-derived growth factor-related protein 3 O... [more]
A0A3S2PJ341.466e-2052.38PWWP domain-containing protein OS=Oryzias javanicu... [more]
A0A3P9HTD81.843e-2051.19HDGF like 3 OS=Oryzias latipes OX=8090 GN=HDGFL3 P... [more]
A0A3B3H9C42.062e-2051.19HDGF like 3 OS=Oryzias latipes OX=8090 GN=HDGFL3 P... [more]
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BLAST of PWWP domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000042912.16.625e-2251.19pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000035361.18.664e-2251.19pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000035815.19.674e-2248.89pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
psip1a1.855e-1948.81PC4 and SFRS1-interacting protein-like [Source:NCB... [more]
psip1a2.138e-1948.81PC4 and SFRS1-interacting protein-like [Source:NCB... [more]
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BLAST of PWWP domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000010402.11.114e-1944.05pep scaffold:Pmarinus_7.0:GL477087:153717:159376:-... [more]
ENSPMAT00000001585.11.352e-628.75pep scaffold:Pmarinus_7.0:GL478812:497860:498661:1... [more]
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BLAST of PWWP domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PWWP domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO420062.163e-1337.80Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of PWWP domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
HDGFL37.761e-2451.19hepatoma-derived growth factor-related protein 3 [... [more]
ENSORLT00000046328.16.349e-2246.07heparin binding growth factor [Source:NCBI gene;Ac... [more]
HDGFL31.603e-2150.60hepatoma-derived growth factor-related protein 3 [... [more]
ENSORLT00000025330.21.093e-2050.00pep primary_assembly:ASM223467v1:1:16538339:165405... [more]
ENSORLT00000021204.33.592e-1644.58HDGF like 2 [Source:NCBI gene;Acc:101158434][more]
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BLAST of PWWP domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000026652.10.000e+099.82SMESG000026652.1[more]
SMESG000026652.10.000e+0100.00SMESG000026652.1[more]
SMESG000026652.10.000e+0100.00SMESG000026652.1[more]
SMESG000069102.15.644e-2041.12SMESG000069102.1[more]
SMESG000057564.15.167e-1840.54SMESG000057564.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013889 ID=SMED30013889|Name=PWWP domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=3590bp
GTTATTTACGAAAAATTTATATTTTAAAATTTATTGTTACATTGATAAAA
TTCGTTATAATATTTTAGCTTCAAAATTGAGGATTTTAAAATAAATGATC
GCGTTTTCGCGAAGTTGCCTAACTGTCCATATTGGCCAGCTAGAATCAAC
AAATTACCAGATTATATTTCTGTGCCTGATGGAAATTATCCTATTTTCTT
TTTCGGTACTCACGAAGTAGAATTTTTGCCCTCAAAATCTCTCGTTCCGT
ATGCGGAAAACAAACAGAAATACATCCGAAACAGTCGATATCAGCATTTC
ACCGAAGCTATATGGGAGATTGAGAACAATTTCGATATTTTGGCACTGGA
TAAGGATCCGGCGGCCGAAAATTTCCTGTGTGAAATGCATGATACCGAGA
AACCAACGGCTAAGATTTCCGATCCAAGTGCCGATTCGAATGCCGAAAAA
GTGATAGATGTGGAAAATCCAGAAATCAACGATGTCGAGGGGATTATGAA
TCTCGGTGAGAATGAGTGGAATGAGATATTCACGATCGAGGAGCAAGAAA
TTCATAACAACCTGTCGCAATTGGCCGATCCAGTATTTGGTAACAGTGTT
AGTGACGCAGCCAATAACACAGCCACCCCAAATGGCCCCGTCAAAACAGC
GATCATTCGGGAAGCAATTTCGATTACCCCAACAAAGATCAAGATCTGTC
AAACATCAGTTCCTGTATCCAATGAGAATCATGAAGCTCAGTCGAGCTTG
CATCGATCCGCACTGCACAACAATTGTAAAGTCATTGTGGAGAAACTGTC
CATCATTCAAATCAATCGAGTCATTCAGAGCAATTTCTCTGTCGGATTAA
TGGAAGATTTGAGTAAGAAGAACCAAAAGAAACCGCCGAGTTTCGTGAGT
TCCAAACCGGCACTGACACCGACACCAGCAAATATTGATCCGAAACCGAA
GAAGGCGGCCATCTCAGTGAATGACAGCAATCCAATATCAACAAAGCCAC
AAATACAACCAGCAACAACTATAGCAGCGACAGCAATAGCACCGGATCCC
AGGAAACCCGCAACGTCCAACAAAATCCTGTCTACAATCAATAAAAACTC
CGTTTCTAGAATTATCGACGCCATCTCCATAGATTCCGATGATAATCAGA
GCTGTGTCATTGTTTCTCCACCAAAAATAAAATCCTCCTCTGAGTTGAAA
CGAAAGCAGGAGAAAATTGAGAATGAATTGAAAATTCTGCCAACGATCAA
ATTGAAGTTGGTGAGGCCGAATCCCGATACAGCTGATAAGAATGACAAGC
TGCCGATAACAGGAGTATCAGGGGGGACTGGCGCAACAGCTGCGGGCAAT
TCCGATTCCAGGAGAAGCGGGAAAATCAAAACAAAGGAAAGAAAAATCTG
TAAGACTGATTATATGGCGACGAGCAGAAACACGAATGATAAGCCGTTGA
CGTTTGACAACCTGTTCAACAAGAAGTTCAGCATCCTTGGTAGCGGTTTG
AAGATAGCCAAGAAAGCGATTGCAGATGTTGTCAAGGAGGATCCGGAATC
GAGTGGAGTCGGTGCAGTGACAGCAAATCAGCAGCAGCGGCAGCAGGACA
CAGAGGAAAACAAACCCATAAAGCAGAATCCGTTTGAGACCGAGGAGGAC
GAGGGAAATTTTTGGCCGAAAATGAAAGAATGTGAGGATGAATTCAACAC
GCAGCCGAAACATAATGGTGGATACGAGGAAACCGGGGGTGACTTTGTCG
ATGATGACGATGACGATGCTCCGTGGCCCGTTGCCAGGACCGAGGAGGAC
ATGGAGCCCAATCGGAACTGTATCAAGCATGAAATGAAGGAAGGCTCAAA
CGATAAAGATGTGGGAACTGAGGAAGAGGAAATGTCTAGTAAATGGTACA
CTAATGGGACCGGCGCAGAGGAGCCGCGGGGTGAATCGGAAATCGATCCT
GAGAACGCTGATAAGAAAGATCCATGGAGTAATTATATGGCAAAGAAACA
AAAGGAGGACAATTTAGTTGGAGAGAATGTTGTTGAGCTTCCAGATAGTG
ACAGCGAGATGTTTGACGTGATATTAAAGGAGGAGCCGGGAAATCCAGTG
AAAAGATCAGCTGGGAATGTGTGGAGCCATATCCAGGAATATCAGAGCCA
ATTCGATAACGGTAAACAGGACGTTATGGACACTATGGACGGTGTTGAAA
TCGGTCCAGATGACGCCATCAGGAACATGGAATACATACAGGACGCCAAA
TGGCTCAATTCGACCAATGAAGACTTCCTTGATTCACCGCCGCTGCCCCA
AGCGATGCCCGATGAAGAGAATGTTTCCTTGTGCCAAAATGTGGACCTTG
CTAGTACGATTGTTCACAAGAAATGGTACGATCGAAACCGGCTGCCTTCT
CCACCGAATAATGTTCCCAGTCTGATGGATATTGACACCAGCCGGGCAAT
GAAAGAGTTCAGGGGCCCTGAATCGTGGGTCGATAGTGGAACTGCTTTCG
GCCGTGCGTTCCGGGAATCATCGCACAACAACCGCGCTAGGAACCGCCAT
ATGTCAACCAATGCAAAATCGAGGAATAATTTCGTTTCTCCCAATTCGAA
AACGGCCGCCTTTCAGAATATCCCTTTCGAGCAACTTTTTCCACCGTTCA
AAGCCGAATACTTCACAAACAATGGCACAAAGGGGAACAATAATCACCAA
TTGTCAACGCCGCAACCGTCGGAGCAGCAACCGGGCAAGGCACAAAATCG
GGCCGGGAAAACCCAGAACCTCAGGCCAACGGATTGCCGAATGCCGGGCA
ATAAACTGCCGGGAATGATGCCTTCTGGTGACCCATATCAATATTACGGC
GACCCCTATCATCACTATGCCCACGGAATGGCCATGGTCGAGGATCCGTA
TTATTACTATTATTATAACAACTATTACTATGGCAACAATAACCATCTAC
TGCAACAACCGTCACCGATGACGTCACGTGTCGCACGTCCCCACTCATCC
GCACCCGTACCAATTACTCCCGAATCACCTGATCCTTACACAGACGATGC
CGCTGATCCCTTGACTGTCGGCAGCAAACCGTCCACTATTGAACCCCACA
AATTAATCGATCCGGTTGCTGCTGCCTCTAATAAAAAACCGCCACCAATG
AGCCAAATGTATTACCCACAACAATTCTCCTACATGACAGAGAATGGCTA
TTACCTGCCCAGTGAAGAGGAAATGTTCGCATATTTCTACTCGTGCTACG
ACGGACACCCTCCTACAGCGGCCGCATTTGGGCCTATGAATCCATATCAC
CAACCGAATGTCCCTGCCAGACCCTATCAGCCATATTCAACAGCCAATGA
TCCGATCACCTACACTCCCGCATCACCGGATCCTGAAGAGATGTGCCCAG
CAAATTCAAATAATCCTTGACATTATTAATGTATCTATCAGCCCAAATTA
TAGAATCTATTAATATTTGAACCTGAGGCACTTTTTGTCTTGTTCACATA
TTCTCTCTAGTCTTTTCTAAGGTTTTGATATGGCATTCCCAATTGTATAA
TATTTGTTGATGAATAAATTTATATAAACTTTGTCAAAAA
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protein sequence of SMED30013889-orf-1

>SMED30013889-orf-1 ID=SMED30013889-orf-1|Name=SMED30013889-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1012bp
MHDTEKPTAKISDPSADSNAEKVIDVENPEINDVEGIMNLGENEWNEIFT
IEEQEIHNNLSQLADPVFGNSVSDAANNTATPNGPVKTAIIREAISITPT
KIKICQTSVPVSNENHEAQSSLHRSALHNNCKVIVEKLSIIQINRVIQSN
FSVGLMEDLSKKNQKKPPSFVSSKPALTPTPANIDPKPKKAAISVNDSNP
ISTKPQIQPATTIAATAIAPDPRKPATSNKILSTINKNSVSRIIDAISID
SDDNQSCVIVSPPKIKSSSELKRKQEKIENELKILPTIKLKLVRPNPDTA
DKNDKLPITGVSGGTGATAAGNSDSRRSGKIKTKERKICKTDYMATSRNT
NDKPLTFDNLFNKKFSILGSGLKIAKKAIADVVKEDPESSGVGAVTANQQ
QRQQDTEENKPIKQNPFETEEDEGNFWPKMKECEDEFNTQPKHNGGYEET
GGDFVDDDDDDAPWPVARTEEDMEPNRNCIKHEMKEGSNDKDVGTEEEEM
SSKWYTNGTGAEEPRGESEIDPENADKKDPWSNYMAKKQKEDNLVGENVV
ELPDSDSEMFDVILKEEPGNPVKRSAGNVWSHIQEYQSQFDNGKQDVMDT
MDGVEIGPDDAIRNMEYIQDAKWLNSTNEDFLDSPPLPQAMPDEENVSLC
QNVDLASTIVHKKWYDRNRLPSPPNNVPSLMDIDTSRAMKEFRGPESWVD
SGTAFGRAFRESSHNNRARNRHMSTNAKSRNNFVSPNSKTAAFQNIPFEQ
LFPPFKAEYFTNNGTKGNNNHQLSTPQPSEQQPGKAQNRAGKTQNLRPTD
CRMPGNKLPGMMPSGDPYQYYGDPYHHYAHGMAMVEDPYYYYYYNNYYYG
NNNHLLQQPSPMTSRVARPHSSAPVPITPESPDPYTDDAADPLTVGSKPS
TIEPHKLIDPVAAASNKKPPPMSQMYYPQQFSYMTENGYYLPSEEEMFAY
FYSCYDGHPPTAAAFGPMNPYHQPNVPARPYQPYSTANDPITYTPASPDP
EEMCPANSNNP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR011528NERD
IPR000313PWWP_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 772..795
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 387..531
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 298..333
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 406..442
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 165..179
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 466..499
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 451..465
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 719..733
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 772..801
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 514..531
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 985..1011
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 162..188
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 710..733