SMED30013884
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30013884 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8Note: Hover over icons to view figure legend
Homology
BLAST of SMED30013884 vs. Planmine SMEST
Match: SMESG000049063.1 (SMESG000049063.1) HSP 1 Score: 1400.57 bits (3624), Expect = 0.000e+0 Identity = 766/775 (98.84%), Postives = 769/775 (99.23%), Query Frame = 2 Query: 38 MTMXXXXXXXXXXXXXXXXXXDVERVYVDWPEIDSEEFRFISSRLEDGEMTPLYFQHHIQDNQYNIFNINQLRFADKSRSEPAANDRYEDLXXXXXXXXXXXXXVPPLDLISQNSSIEIPGAKGVIDIXXXXXXXXXXXXXXXYKNQSLQNFERDSLDVENINHFNENKGNQSYPLLSTTLPEIQHSDLMLNPGEYDTSRYNKPASNVSRKFSEISVASQYKSELSSAESHHVQRLENEKAIFEAAKQKYKQTVSKSSQNSAESHQPEYIQELSPAKNLKSKPTMLVAPQIKQQLYNTDKTPNFVELNKISTCPQKQSNQTSYASKFIERHHKESPKPVLKKSESFKDSIHYQNMSGEKLNSDIPHEFSGEEYLNKRVKSVDRFMRDNPNGDNHELPIINSGFPANQTSDSSKLVSPPHFIYAYNQYGEAMIINTEKVVGHYHNTNLDHSHFHSPYNQNQPQYYPSPNTINENRSQSNSPQINHKAYHSQEIHKPAEPEFVFKDANYKRQPTLYDESLLTRKFKEITTERSGSRFPPIPNTTDSTQSQTTGYASHHKKKEISYEQYTLRDFRNIKQVERGGGLGPNIGSDSYKQLKEKLIRQKNYSKAVLEKNRQELIKTNEKTSREKPMKSDTSTENISFQNVQDVLVSTRDNDKLEAVLHKPLEQTSNEQVTIQREETKSSNFQKMGSASSKELINKREAMLNYAXXXXXXXXXXXXXXXHGSKVHSANIKKEVEKSAIHELINNSDLDRLLQRHEDDKKIADQIRIKLGKIS 2362 MTMFNGSSEKEFSEKNFETNEDVERVYVDWPEIDSEEFRFISSRLEDGEMTPLYFQHHIQDNQYNIF+INQLRFADKSRSEPAANDRYEDLSKKKISKKLKNSSVPPLDLISQNSSIEIPGAKGVIDIDLRSSSESLSDDSEEYKNQSLQNFERDSLDVENINHFNENKGNQSYPLLSTTLPEIQHSDLMLNPGEYDTSRYNKPASNVSRKFSEISVASQYKSELSSAESHHVQRLENEKAIFEAAKQKYKQ VSKSSQNSAESHQPEYI E SPAKNLKSKPTMLVAPQIKQQLYNTDKTPNFVELNKISTCP KQSNQTSYASKFIERHHKESPKPVLKKSESFKDSIHYQNM+GEKLNSDIPHEFSGEEYLNKRVKSVDR MRDN NGDNHELPIINSGFPANQTSDSSKLV+PPHFIYAYNQYGEAMIINTEKVVGHYHNTNLDHSHFHSPYNQNQPQYYPSPNTINENRSQSNSPQINHKAYHSQEIHKPAEPEFVFKDANYKRQPTLYDESLLTRKFKEITTERSGSRFPPIPNTTDSTQSQTTGYASHHKKKEISYEQYTLRDFRNIKQVERGGGLGPNIGSDSYKQLKEKLIRQKNYSKAVLEKNRQELIKTNEKTSREKPMKSDTSTENISFQNVQDVLVSTRDNDKLEAVLHKPLEQTSNEQVTIQREETKSSNFQKMGSASSKELINKREAMLNYAKSIQEKQIRKKEIKSHGSKVHSANIKKEVEKSAIHELINNSDLDRLLQRHEDDKKIADQIRIKLGKIS Sbjct: 1 MTMFNGSSEKEFSEKNFETNEDVERVYVDWPEIDSEEFRFISSRLEDGEMTPLYFQHHIQDNQYNIFDINQLRFADKSRSEPAANDRYEDLSKKKISKKLKNSSVPPLDLISQNSSIEIPGAKGVIDIDLRSSSESLSDDSEEYKNQSLQNFERDSLDVENINHFNENKGNQSYPLLSTTLPEIQHSDLMLNPGEYDTSRYNKPASNVSRKFSEISVASQYKSELSSAESHHVQRLENEKAIFEAAKQKYKQNVSKSSQNSAESHQPEYIPEHSPAKNLKSKPTMLVAPQIKQQLYNTDKTPNFVELNKISTCPHKQSNQTSYASKFIERHHKESPKPVLKKSESFKDSIHYQNMAGEKLNSDIPHEFSGEEYLNKRVKSVDRLMRDNLNGDNHELPIINSGFPANQTSDSSKLVNPPHFIYAYNQYGEAMIINTEKVVGHYHNTNLDHSHFHSPYNQNQPQYYPSPNTINENRSQSNSPQINHKAYHSQEIHKPAEPEFVFKDANYKRQPTLYDESLLTRKFKEITTERSGSRFPPIPNTTDSTQSQTTGYASHHKKKEISYEQYTLRDFRNIKQVERGGGLGPNIGSDSYKQLKEKLIRQKNYSKAVLEKNRQELIKTNEKTSREKPMKSDTSTENISFQNVQDVLVSTRDNDKLEAVLHKPLEQTSNEQVTIQREETKSSNFQKMGSASSKELINKREAMLNYAKSIQEKQIRKKEIKSHGSKVHSANIKKEVEKSAIHELINNSDLDRLLQRHEDDKKIADQIRIKLGKIS 775
BLAST of SMED30013884 vs. Planmine SMEST
Match: SMESG000049063.1 (SMESG000049063.1) HSP 1 Score: 1400.57 bits (3624), Expect = 0.000e+0 Identity = 745/754 (98.81%), Postives = 748/754 (99.20%), Query Frame = 2 Query: 101 DVERVYVDWPEIDSEEFRFISSRLEDGEMTPLYFQHHIQDNQYNIFNINQLRFADKSRSEPAANDRYEDLXXXXXXXXXXXXXVPPLDLISQNSSIEIPGAKGVIDIXXXXXXXXXXXXXXXYKNQSLQNFERDSLDVENINHFNENKGNQSYPLLSTTLPEIQHSDLMLNPGEYDTSRYNKPASNVSRKFSEISVASQYKSELSSAESHHVQRLENEKAIFEAAKQKYKQTVSKSSQNSAESHQPEYIQELSPAKNLKSKPTMLVAPQIKQQLYNTDKTPNFVELNKISTCPQKQSNQTSYASKFIERHHKESPKPVLKKSESFKDSIHYQNMSGEKLNSDIPHEFSGEEYLNKRVKSVDRFMRDNPNGDNHELPIINSGFPANQTSDSSKLVSPPHFIYAYNQYGEAMIINTEKVVGHYHNTNLDHSHFHSPYNQNQPQYYPSPNTINENRSQSNSPQINHKAYHSQEIHKPAEPEFVFKDANYKRQPTLYDESLLTRKFKEITTERSGSRFPPIPNTTDSTQSQTTGYASHHKKKEISYEQYTLRDFRNIKQVERGGGLGPNIGSDSYKQLKEKLIRQKNYSKAVLEKNRQELIKTNEKTSREKPMKSDTSTENISFQNVQDVLVSTRDNDKLEAVLHKPLEQTSNEQVTIQREETKSSNFQKMGSASSKELINKREAMLNYAXXXXXXXXXXXXXXXHGSKVHSANIKKEVEKSAIHELINNSDLDRLLQRHEDDKKIADQIRIKLGKIS 2362 DVERVYVDWPEIDSEEFRFISSRLEDGEMTPLYFQHHIQDNQYNIF+INQLRFADKSRSEPAANDRYEDLSKKKISKKLKNSSVPPLDLISQNSSIEIPGAKGVIDIDLRSSSESLSDDSEEYKNQSLQNFERDSLDVENINHFNENKGNQSYPLLSTTLPEIQHSDLMLNPGEYDTSRYNKPASNVSRKFSEISVASQYKSELSSAESHHVQRLENEKAIFEAAKQKYKQ VSKSSQNSAESHQPEYI E SPAKNLKSKPTMLVAPQIKQQLYNTDKTPNFVELNKISTCP KQSNQTSYASKFIERHHKESPKPVLKKSESFKDSIHYQNM+GEKLNSDIPHEFSGEEYLNKRVKSVDR MRDN NGDNHELPIINSGFPANQTSDSSKLV+PPHFIYAYNQYGEAMIINTEKVVGHYHNTNLDHSHFHSPYNQNQPQYYPSPNTINENRSQSNSPQINHKAYHSQEIHKPAEPEFVFKDANYKRQPTLYDESLLTRKFKEITTERSGSRFPPIPNTTDSTQSQTTGYASHHKKKEISYEQYTLRDFRNIKQVERGGGLGPNIGSDSYKQLKEKLIRQKNYSKAVLEKNRQELIKTNEKTSREKPMKSDTSTENISFQNVQDVLVSTRDNDKLEAVLHKPLEQTSNEQVTIQREETKSSNFQKMGSASSKELINKREAMLNYAKSIQEKQIRKKEIKSHGSKVHSANIKKEVEKSAIHELINNSDLDRLLQRHEDDKKIADQIRIKLGKIS Sbjct: 20 DVERVYVDWPEIDSEEFRFISSRLEDGEMTPLYFQHHIQDNQYNIFDINQLRFADKSRSEPAANDRYEDLSKKKISKKLKNSSVPPLDLISQNSSIEIPGAKGVIDIDLRSSSESLSDDSEEYKNQSLQNFERDSLDVENINHFNENKGNQSYPLLSTTLPEIQHSDLMLNPGEYDTSRYNKPASNVSRKFSEISVASQYKSELSSAESHHVQRLENEKAIFEAAKQKYKQNVSKSSQNSAESHQPEYIPEHSPAKNLKSKPTMLVAPQIKQQLYNTDKTPNFVELNKISTCPHKQSNQTSYASKFIERHHKESPKPVLKKSESFKDSIHYQNMAGEKLNSDIPHEFSGEEYLNKRVKSVDRLMRDNLNGDNHELPIINSGFPANQTSDSSKLVNPPHFIYAYNQYGEAMIINTEKVVGHYHNTNLDHSHFHSPYNQNQPQYYPSPNTINENRSQSNSPQINHKAYHSQEIHKPAEPEFVFKDANYKRQPTLYDESLLTRKFKEITTERSGSRFPPIPNTTDSTQSQTTGYASHHKKKEISYEQYTLRDFRNIKQVERGGGLGPNIGSDSYKQLKEKLIRQKNYSKAVLEKNRQELIKTNEKTSREKPMKSDTSTENISFQNVQDVLVSTRDNDKLEAVLHKPLEQTSNEQVTIQREETKSSNFQKMGSASSKELINKREAMLNYAKSIQEKQIRKKEIKSHGSKVHSANIKKEVEKSAIHELINNSDLDRLLQRHEDDKKIADQIRIKLGKIS 773
BLAST of SMED30013884 vs. Planmine SMEST
Match: SMESG000049063.1 (SMESG000049063.1) HSP 1 Score: 1024.23 bits (2647), Expect = 0.000e+0 Identity = 530/540 (98.15%), Postives = 533/540 (98.70%), Query Frame = 2 Query: 743 LENEKAIFEAAKQKYKQTVSKSSQNSAESHQPEYIQELSPAKNLKSKPTMLVAPQIKQQLYNTDKTPNFVELNKISTCPQKQSNQTSYASKFIERHHKESPKPVLKKSESFKDSIHYQNMSGEKLNSDIPHEFSGEEYLNKRVKSVDRFMRDNPNGDNHELPIINSGFPANQTSDSSKLVSPPHFIYAYNQYGEAMIINTEKVVGHYHNTNLDHSHFHSPYNQNQPQYYPSPNTINENRSQSNSPQINHKAYHSQEIHKPAEPEFVFKDANYKRQPTLYDESLLTRKFKEITTERSGSRFPPIPNTTDSTQSQTTGYASHHKKKEISYEQYTLRDFRNIKQVERGGGLGPNIGSDSYKQLKEKLIRQKNYSKAVLEKNRQELIKTNEKTSREKPMKSDTSTENISFQNVQDVLVSTRDNDKLEAVLHKPLEQTSNEQVTIQREETKSSNFQKMGSASSKELINKREAMLNYAXXXXXXXXXXXXXXXHGSKVHSANIKKEVEKSAIHELINNSDLDRLLQRHEDDKKIADQIRIKLGKIS 2362 +NEKAIFEAAKQKYKQ VSKSSQNSAESHQPEYI E SPAKNLKSKPTMLVAPQIKQQLYNTDKTPNFVELNKISTCP KQSNQTSYASKFIERHHKESPKPVLKKSESFKDSIHYQNM+GEKLNSDIPHEFSGEEYLNKRVKSVDR MRDN NGDNHELPIINSGFPANQTSDSSKLV+PPHFIYAYNQYGEAMIINTEKVVGHYHNTNLDHSHFHSPYNQNQPQYYPSPNTINENRSQSNSPQINHKAYHSQEIHKPAEPEFVFKDANYKRQPTLYDESLLTRKFKEITTERSGSRFPPIPNTTDSTQSQTTGYASHHKKKEISYEQYTLRDFRNIKQVERGGGLGPNIGSDSYKQLKEKLIRQKNYSKAVLEKNRQELIKTNEKTSREKPMKSDTSTENISFQNVQDVLVSTRDNDKLEAVLHKPLEQTSNEQVTIQREETKSSNFQKMGSASSKELINKREAMLNYAKSIQEKQIRKKEIKSHGSKVHSANIKKEVEKSAIHELINNSDLDRLLQRHEDDKKIADQIRIKLGKIS Sbjct: 28 FKNEKAIFEAAKQKYKQNVSKSSQNSAESHQPEYIPEHSPAKNLKSKPTMLVAPQIKQQLYNTDKTPNFVELNKISTCPHKQSNQTSYASKFIERHHKESPKPVLKKSESFKDSIHYQNMAGEKLNSDIPHEFSGEEYLNKRVKSVDRLMRDNLNGDNHELPIINSGFPANQTSDSSKLVNPPHFIYAYNQYGEAMIINTEKVVGHYHNTNLDHSHFHSPYNQNQPQYYPSPNTINENRSQSNSPQINHKAYHSQEIHKPAEPEFVFKDANYKRQPTLYDESLLTRKFKEITTERSGSRFPPIPNTTDSTQSQTTGYASHHKKKEISYEQYTLRDFRNIKQVERGGGLGPNIGSDSYKQLKEKLIRQKNYSKAVLEKNRQELIKTNEKTSREKPMKSDTSTENISFQNVQDVLVSTRDNDKLEAVLHKPLEQTSNEQVTIQREETKSSNFQKMGSASSKELINKREAMLNYAKSIQEKQIRKKEIKSHGSKVHSANIKKEVEKSAIHELINNSDLDRLLQRHEDDKKIADQIRIKLGKIS 567
BLAST of SMED30013884 vs. Planmine SMEST
Match: SMESG000049207.1 (SMESG000049207.1) HSP 1 Score: 628.632 bits (1620), Expect = 0.000e+0 Identity = 303/312 (97.12%), Postives = 305/312 (97.76%), Query Frame = 2 Query: 749 NEKAIFEAAKQKYKQTVSKSSQNSAESHQPEYIQELSPAKNLKSKPTMLVAPQIKQQLYNTDKTPNFVELNKISTCPQKQSNQTSYASKFIERHHKESPKPVLKKSESFKDSIHYQNMSGEKLNSDIPHEFSGEEYLNKRVKSVDRFMRDNPNGDNHELPIINSGFPANQTSDSSKLVSPPHFIYAYNQYGEAMIINTEKVVGHYHNTNLDHSHFHSPYNQNQPQYYPSPNTINENRSQSNSPQINHKAYHSQEIHKPAEPEFVFKDANYKRQPTLYDESLLTRKFKEITTERSGSRFPPIPNTTDSTQSQT 1684 NEKAIFEAAKQKYKQ VSKSSQNSAESHQPEYIQE SPAKNLKSKPTMLVAPQIKQQLYNTDKTPNFVELNKISTCP KQSNQTSYASKFIERHHKESPKPVLKKSESFKDSIHYQNM+GEKLNSDIPHEFSGEEYLNKRVKSVDR MRDN NGDNHELPIINSGFPANQTSDSSKLV+PPHFIYAYNQYGEAMIINTEKVVGHYHNTNLDHSHFHSPYNQNQPQYYPSPNTINENRSQSNSPQINHKAYHSQEIHKPAEPEF FKDANYKRQPTLYDESLLTRKFKEITTERSGSRFPPIPNTTDSTQSQ Sbjct: 225 NEKAIFEAAKQKYKQNVSKSSQNSAESHQPEYIQEHSPAKNLKSKPTMLVAPQIKQQLYNTDKTPNFVELNKISTCPHKQSNQTSYASKFIERHHKESPKPVLKKSESFKDSIHYQNMAGEKLNSDIPHEFSGEEYLNKRVKSVDRLMRDNLNGDNHELPIINSGFPANQTSDSSKLVNPPHFIYAYNQYGEAMIINTEKVVGHYHNTNLDHSHFHSPYNQNQPQYYPSPNTINENRSQSNSPQINHKAYHSQEIHKPAEPEFAFKDANYKRQPTLYDESLLTRKFKEITTERSGSRFPPIPNTTDSTQSQV 536
BLAST of SMED30013884 vs. Planmine SMEST
Match: SMESG000049205.1 (SMESG000049205.1) HSP 1 Score: 201.445 bits (511), Expect = 1.199e-59 Identity = 103/117 (88.03%), Postives = 107/117 (91.45%), Query Frame = 2 Query: 1796 IGSDSYKQLKEKLIRQKNYSKAVLEKNRQELIKTNEKTSREKPMKSDTSTENISFQNVQDVLVSTRDNDKLEAVLHKPLEQTSNEQVTIQREETKSSNFQKMGSASSKELINKREAM 2146 D K+ KEKLIRQKNYSKAVLEKNRQELIKT EKTS EKPMKSDTSTENISFQN+QDVLVS RDNDKLEAVLHKPLEQTSNEQV IQREETKSSNFQKMGSASSKELINKR+A+ Sbjct: 87 CCRDYKKKEKEKLIRQKNYSKAVLEKNRQELIKTKEKTSSEKPMKSDTSTENISFQNLQDVLVSARDNDKLEAVLHKPLEQTSNEQVKIQREETKSSNFQKMGSASSKELINKRDAV 203 The following BLAST results are available for this feature:
BLAST of SMED30013884 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30013884 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30013884 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30013884 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30013884 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30013884 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30013884 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30013884 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30013884 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30013884 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30013884 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30013884 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30013884 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30013884 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30013884 ID=SMED30013884|Name=SMED30013884|organism=Schmidtea mediterranea sexual|type=transcript|length=2411bpback to top protein sequence of SMED30013884-orf-1 >SMED30013884-orf-1 ID=SMED30013884-orf-1|Name=SMED30013884-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=776bp MTMFNGSSEKEFSEKNFETNEDVERVYVDWPEIDSEEFRFISSRLEDGEMback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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