Transcription factor Adf-1

Overview
NameTranscription factor Adf-1
Smed IDSMED30013881
Length (bp)1179
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Transcription factor Adf-1 (SMED30013881) t-SNE clustered cells

Violin plots show distribution of expression levels for Transcription factor Adf-1 (SMED30013881) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Transcription factor Adf-1 (SMED30013881) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Transcription factor Adf-1 (SMED30013881) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013881

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 21

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30013881h1SMcG0010730 SmedASXL_014954SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30013881h1SMcG0010730 Contig51307uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013881h1SMcG0010730 Contig51307newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
muscle cellSMED30013881h1SMcG0010730 dd_Smed_v4_9808_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30013881h1SMcG0010730 dd_Smed_v4_99_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30013881h1SMcG0010730 dd_Smed_v4_99_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30013881h1SMcG0010730 dd_Smed_v4_99_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30013881h1SMcG0010730 dd_Smed_v4_99_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30013881h1SMcG0010730 dd_Smed_v4_99_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30013881h1SMcG0010730 dd_Smed_v4_99_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30013881h1SMcG0010730 dd_Smed_v4_99_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30013881h1SMcG0010730 dd_Smed_v4_99_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30013881h1SMcG0010730 dd_Smed_v4_99_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30013881h1SMcG0010730 dd_Smed_v4_99_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30013881h1SMcG0010730 dd_Smed_v4_4755_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30013881h1SMcG0010730 dd_Smed_v4_4755_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30013881h1SMcG0010730 dd_Smed_v6_9808_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
body wall musculatureSMED30013881h1SMcG0010730 dd_Smed_v4_99_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30013881h1SMcG0010730 dd_Smed_v6_99_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 2 cellSMED30013881h1SMcG0010730 dd_Smed_v4_99_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30013881h1SMcG0010730 dd_Smed_v4_4755_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of Transcription factor Adf-1 vs. Ensembl Celegans
Match: madf-5 (MADF domain transcription factor [Source:UniProtKB/TrEMBL;Acc:O45233])

HSP 1 Score: 48.1358 bits (113), Expect = 8.040e-6
Identity = 38/125 (30.40%), Postives = 59/125 (47.20%), Query Frame = 1 
Query:  364 FEAKLIEELKQHPNLY--TKRSQLDFHEYNTIEQSWEIVAKKMDSDTKT--CKKIWRNLRDSYVRQLKKISCSADIGIDTSQFT--KWKHFEKMS----FLKNVISHRTINESKSSDQNSMSEQT 708
            F A+LI E++++P LY  ++R   D  E   +   WE +AK +D +      KK W  LRD Y ++LK       I I     T  +W +F ++S    FLK+ I        K+   +S  E +
Sbjct:    7 FNARLINEVRKYPCLYNHSRRGSGDTMERQRL---WESIAKNIDPNCAAEFAKKRWLQLRDRYRKELK-------IAIKNGFVTPVRWCYFNQLSWLDPFLKDNIGQAADEGKKTGKSDSFDEPS 121
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BLAST of Transcription factor Adf-1 vs. Ensembl Fly
Match: CG7745 (gene:FBgn0033616 transcript:FBtr0088119)

HSP 1 Score: 55.4546 bits (132), Expect = 4.961e-8
Identity = 31/104 (29.81%), Postives = 55/104 (52.88%), Query Frame = 1 
Query:  361 LFEAKLIEELKQHPNLYTKR-----SQLDFHEYNTIEQSWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKKISCSADIGIDTSQFTKWKHFEKMSFLKNVISHR 657
            + + +LI+E+ QH  +Y ++        +  +Y T +++W+++A K+ +D  TCKK W+ LR+ YV Q K+     D  +   +     + EKM FL   I  R
Sbjct:    1 MMDEQLIDEVAQHGVIYNRQKYYLNGGANGGKYETKDEAWQLIAMKLRTDVDTCKKRWKYLRERYVSQRKQ----GDPPV--YEHLSRPYLEKMKFLDQHIQPR 98
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BLAST of Transcription factor Adf-1 vs. Ensembl Fly
Match: Adf1 (gene:FBgn0284249 transcript:FBtr0330252)

HSP 1 Score: 53.9138 bits (128), Expect = 7.380e-8
Identity = 31/115 (26.96%), Postives = 60/115 (52.17%), Query Frame = 1 
Query:  364 FEAKLIEELKQHPNLYTKRSQLDFHEYNTIEQSWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKKISCSADIGIDTSQFTKWKHFEKMSFLKNVISH-------RTINESKSSDQ 687
            F+  LIE +K +P +Y  RS  ++  +    Q+W+ +A+ +    + C K W++LRD + R++K             Q ++W++F++M FL + I         +  N S+S++Q
Sbjct:   12 FDLNLIEAVKLNPVIY-DRSHYNYKHFVRKAQTWKQIAETLGVPEQKCTKRWKSLRDKFAREMK-----------LCQESRWRYFKQMQFLVDSIRQYRESLLGKCANGSQSANQ 114
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BLAST of Transcription factor Adf-1 vs. Ensembl Fly
Match: Adf1 (gene:FBgn0284249 transcript:FBtr0086112)

HSP 1 Score: 53.5286 bits (127), Expect = 8.050e-8
Identity = 31/115 (26.96%), Postives = 60/115 (52.17%), Query Frame = 1 
Query:  364 FEAKLIEELKQHPNLYTKRSQLDFHEYNTIEQSWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKKISCSADIGIDTSQFTKWKHFEKMSFLKNVISH-------RTINESKSSDQ 687
            F+  LIE +K +P +Y  RS  ++  +    Q+W+ +A+ +    + C K W++LRD + R++K             Q ++W++F++M FL + I         +  N S+S++Q
Sbjct:   12 FDLNLIEAVKLNPVIY-DRSHYNYKHFVRKAQTWKQIAETLGVPEQKCTKRWKSLRDKFAREMK-----------LCQESRWRYFKQMQFLVDSIRQYRESLLGKCANGSQSANQ 114
[Close]
BLAST of Transcription factor Adf-1 vs. Ensembl Fly
Match: Adf1 (gene:FBgn0284249 transcript:FBtr0086111)

HSP 1 Score: 53.5286 bits (127), Expect = 8.050e-8
Identity = 31/115 (26.96%), Postives = 60/115 (52.17%), Query Frame = 1 
Query:  364 FEAKLIEELKQHPNLYTKRSQLDFHEYNTIEQSWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKKISCSADIGIDTSQFTKWKHFEKMSFLKNVISH-------RTINESKSSDQ 687
            F+  LIE +K +P +Y  RS  ++  +    Q+W+ +A+ +    + C K W++LRD + R++K             Q ++W++F++M FL + I         +  N S+S++Q
Sbjct:   12 FDLNLIEAVKLNPVIY-DRSHYNYKHFVRKAQTWKQIAETLGVPEQKCTKRWKSLRDKFAREMK-----------LCQESRWRYFKQMQFLVDSIRQYRESLLGKCANGSQSANQ 114
[Close]
BLAST of Transcription factor Adf-1 vs. Ensembl Fly
Match: Adf1 (gene:FBgn0284249 transcript:FBtr0086113)

HSP 1 Score: 53.5286 bits (127), Expect = 8.654e-8
Identity = 31/115 (26.96%), Postives = 60/115 (52.17%), Query Frame = 1 
Query:  364 FEAKLIEELKQHPNLYTKRSQLDFHEYNTIEQSWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKKISCSADIGIDTSQFTKWKHFEKMSFLKNVISH-------RTINESKSSDQ 687
            F+  LIE +K +P +Y  RS  ++  +    Q+W+ +A+ +    + C K W++LRD + R++K             Q ++W++F++M FL + I         +  N S+S++Q
Sbjct:   21 FDLNLIEAVKLNPVIY-DRSHYNYKHFVRKAQTWKQIAETLGVPEQKCTKRWKSLRDKFAREMK-----------LCQESRWRYFKQMQFLVDSIRQYRESLLGKCANGSQSANQ 123
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BLAST of Transcription factor Adf-1 vs. Ensembl Zebrafish
Match: si:zfos-128g4.1 (si:zfos-128g4.1 [Source:ZFIN;Acc:ZDB-GENE-141212-382])

HSP 1 Score: 50.447 bits (119), Expect = 1.209e-6
Identity = 50/246 (20.33%), Postives = 95/246 (38.62%), Query Frame = 1 
Query:  373 KLIEELKQHPNLYTKRSQLDFHEYNTIEQ---SWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKKISCSADIGIDTSQFTKWKHFEKMSFLKNVISHRTINESKSS----------------DQNSMSEQTL----KIESTKLESNSISSDPVSDKETFQNDSRSFIDDETNGDISNSFLCKTSNSISVTNFRSPINE-----------EAFGHHIARSLENLSAIRRSVAKVKIQEIL--SEFEQ 1002
            KLI+ +   P LY     +  H+Y + E+   +W  VA  +      CK+ W+ +RD Y+R+ +      D+G    +   W H E ++FL   I  R                        D+  +SE+ +    ++  + L  + +S+ P    +     S   +     G          + S S      P+ E           + F      +L+ L+  +R+  K++IQ+I+  +EF++
Sbjct:    6 KLIQTVYAFPVLYN----VSLHDYRSTERRVKAWREVAASVGLSVVECKRRWKTIRDRYIRERRLCKLKKDLG--GRRLHYWPHRESLAFLDAHIRKRRRPSGAQGPEEEQQEEHSSAALQEDKECVSEECVDSGSRLAVSPLPVSIMSAPPPPQLKAVPQVSPLLLAALPPGLKVAPVCSSATGSASAGPLNVPLEEQQRADGALDEDQLFLLSYVPALKRLTPQKRAAVKMQIQQIMFNAEFKE 245
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BLAST of Transcription factor Adf-1 vs. Ensembl Zebrafish
Match: LO017799.1 (transcription factor Adf-1-like [Source:NCBI gene;Acc:110439798])

HSP 1 Score: 50.0618 bits (118), Expect = 1.299e-6
Identity = 28/98 (28.57%), Postives = 46/98 (46.94%), Query Frame = 1 
Query:  373 KLIEELKQHPNLYTKRSQLDFHEYNTIEQ---SWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKKISCSADIGIDTSQFTKWKHFEKMSFLKNVISHR 657
            KLI+ +   P LY     +  H+Y + E+   +W  VA  +      CK+ W+ +RD Y+R+ +      D+G    +   W H E ++FL   I  R
Sbjct:    6 KLIQTVYAFPVLYN----VSLHDYRSTERRVKAWREVAASVGLSVVECKRRWKTIRDRYIRERRLCKLKKDLG--GRRLHYWPHRESLAFLDAHIRKR 97
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BLAST of Transcription factor Adf-1 vs. Ensembl Zebrafish
Match: si:ch211-207i20.3 (si:ch211-207i20.3 [Source:ZFIN;Acc:ZDB-GENE-141222-71])

HSP 1 Score: 48.1358 bits (113), Expect = 6.204e-6
Identity = 45/186 (24.19%), Postives = 81/186 (43.55%), Query Frame = 1 
Query:  454 EQSWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKKISCSADIGIDTSQFTKWKHFEKMSFLKNVISHRT-INESKSSDQNSMSEQTLKIESTKLESNSISSDPVSDKETFQNDSRSFIDDETNGDISNSFLCKTSNSISVTNFRSPINEEAFGHHIARSLENLSAIRRSVAKVKIQEIL--SEFEQ 1002
            E  W+ +A KM  D +  K  W+NLRD+Y R+ +     +  G    +  +WK+   M FL     HR+ I +SK  D     ++    E     + +  + P + + +     RS        ++   +L          + +     + F   +A +L  L A ++S+ K++IQ+IL  +EF Q
Sbjct:   50 EALWQGIADKMGVDVEEVKAKWKNLRDTYTRKKRLEQDGSRSGRAAKKKKQWKYMRVMDFLDPATEHRSGILDSKIEDDE--PDEDSGAEPASTSTGTSVTSPEAMRSSIVKRRRS-----ETLELLEKYLATKDAKDREKDEQQQDEVDLFLRSLAPALRRLPASKQSLVKLQIQKILHDAEFGQ 228
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BLAST of Transcription factor Adf-1 vs. UniProt/SwissProt
Match: sp|P05552|ADF1_DROME (Transcription factor Adf-1 OS=Drosophila melanogaster OX=7227 GN=Adf1 PE=2 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 8.675e-7
Identity = 31/115 (26.96%), Postives = 60/115 (52.17%), Query Frame = 1 
Query:  364 FEAKLIEELKQHPNLYTKRSQLDFHEYNTIEQSWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKKISCSADIGIDTSQFTKWKHFEKMSFLKNVISH-------RTINESKSSDQ 687
            F+  LIE +K +P +Y  RS  ++  +    Q+W+ +A+ +    + C K W++LRD + R++K             Q ++W++F++M FL + I         +  N S+S++Q
Sbjct:   21 FDLNLIEAVKLNPVIY-DRSHYNYKHFVRKAQTWKQIAETLGVPEQKCTKRWKSLRDKFAREMK-----------LCQESRWRYFKQMQFLVDSIRQYRESLLGKCANGSQSANQ 123
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BLAST of Transcription factor Adf-1 vs. TrEMBL
Match: A0A3R7M2E3 (Transcription factor Adf-1 OS=Penaeus vannamei OX=6689 GN=C7M84_010650 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 3.395e-11
Identity = 60/226 (26.55%), Postives = 100/226 (44.25%), Query Frame = 1 
Query:  367 EAKLIEELKQHPNLYTKRSQLDFHEYNTIEQSWEIVAKKMDSDT--KTCKKIWRNLRDSYVRQLKKISCSADIGIDTSQFTKWKHFEKMSFLKNVISHRTINESKSSDQNSMSEQTLK---IESTKLESN-SISSDPV------SDKETFQNDSRSFIDDETNGDISNSFLCKTSNSISVTNFRSPINEEAFGHHIARSLENLSAIRRSVAKVKIQEILSEFEQED 1008
            E  LIE ++Q+P+LY  R + D+ +      +W  +A+ +      K CK+ WR LRD YVR  K +        D     +W+ F++M FL + I H+       +  +  S+  +    +E+   E + +I S P        D++T  N +                      +     F     EE FG ++A ++  LSA  +S+AK+KIQ +L E E+ D
Sbjct:  138 ETDLIEHVRQYPHLYDTRHK-DYKDLRKCAVTWAEIARSLGCPEAWKACKERWRTLRDVYVRNKKSVMVG-----DKEGRPRWRFFDQMQFLNSYIIHKPTESDLYASLSGSSKWVVAAADVETVLCEDDTAILSLPFEAHHHDEDEDTTSNPAPPTPRPPRPPTTPTEDAKAPRRAAPPQVFVPQEEEELFGSYVAAAIRKLSAKAKSLAKMKIQRVLFEIEEWD 357
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BLAST of Transcription factor Adf-1 vs. TrEMBL
Match: A0A1B0AMX9 (MADF domain-containing protein OS=Glossina palpalis gambiensis OX=67801 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 5.077e-9
Identity = 35/101 (34.65%), Postives = 57/101 (56.44%), Query Frame = 1 
Query:  361 LFEAKLIEELKQHPNLYTKRSQLDFHEYNTIEQSWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKK--ISCSADIGIDTSQFTKWKHFEKMSFLKNVISHR 657
            +FE KLI  +++ P +Y +R    F +    E++WEI++K++    + CK  WR LRD + R  KK  ISC    G  + +  KWK ++ M FL + I+ +
Sbjct:  263 MFERKLINAVRRKPQVYNRRFP-GFRKPAVKEKAWEIISKEVGMSIELCKGRWRTLRDRFTRDFKKKIISC----GASSPEIHKWKFYDDMLFLGDCIAPK 358
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BLAST of Transcription factor Adf-1 vs. TrEMBL
Match: A0A1A9XA61 (MADF domain-containing protein OS=Glossina fuscipes fuscipes OX=201502 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 5.705e-9
Identity = 35/99 (35.35%), Postives = 56/99 (56.57%), Query Frame = 1 
Query:  361 LFEAKLIEELKQHPNLYTKRSQLDFHEYNTIEQSWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKK--ISCSADIGIDTSQFTKWKHFEKMSFLKNVIS 651
            +FE KLI  +++ P +Y +R    F +    E++WEI++K++    + CK  WR LRD + R  KK  ISC    G  + +  KWK ++ M FL + I+
Sbjct:  263 MFERKLINAVRRKPQVYNRRFP-GFRKPAVKEKAWEIISKEVGMSIELCKGRWRTLRDRFTRDFKKKIISC----GASSPEIHKWKFYDDMLFLGDCIA 356
[Close]
BLAST of Transcription factor Adf-1 vs. TrEMBL
Match: A0A1A9VUR5 (MADF domain-containing protein OS=Glossina austeni OX=7395 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 9.541e-9
Identity = 43/133 (32.33%), Postives = 71/133 (53.38%), Query Frame = 1 
Query:  361 LFEAKLIEELKQHPNLYTKRSQLDFHEYNTIEQSWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKK--ISCSADIGIDTSQFTKWKHFEKMSFLKNVISHRTINESKSSDQNSMSEQTLK-IESTKLESNSIS 750
            +FE KLI  +++ P +Y +R    F +    E++WE ++K++   T+ CK  WR LRD + R  KK  ISC    G  + +  KWK ++ M FL + I+      +K ++ N  S Q ++ IE     S++ S
Sbjct:    1 MFEKKLISAVRRKPEVYNRRFP-GFRKPAVKEKAWETISKEVGMSTEHCKGRWRTLRDRFTRDFKKKTISC----GPSSPESHKWKFYDDMLFLGDCIA------AKRTETNFDSRQLVEFIEVPAPPSDAFS 122
[Close]
BLAST of Transcription factor Adf-1 vs. TrEMBL
Match: A0A0A9X9C2 (Transcription factor Adf-1 (Fragment) OS=Lygus hesperus OX=30085 GN=Adf1_26 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.274e-8
Identity = 37/102 (36.27%), Postives = 59/102 (57.84%), Query Frame = 1 
Query:  364 FEAKLIEELKQHPNLYTKRSQLDFHEYNTIEQSWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKKISC--SADIG-IDTSQFT-KWKHFEKMSFLKNVISHR 657
            FE  LI E++  P L+ +R +  +   N + + WE +AKK++  + T K  WRNLRD++ +++KKI    S D G  +   +T KW  F  +SFL +V+  R
Sbjct:   23 FEESLISEVQSRPILWDRRCE-HYKNRNKVPEEWEKIAKKLNKPSHTVKNKWRNLRDTFQKEMKKIPTYSSGDAGSPNLKNYTGKWIFFNDLSFLTDVMKPR 123
[Close]
BLAST of Transcription factor Adf-1 vs. Planmine SMEST
Match: SMESG000039927.1 (SMESG000039927.1)

HSP 1 Score: 483.411 bits (1243), Expect = 2.263e-173
Identity = 236/237 (99.58%), Postives = 236/237 (99.58%), Query Frame = 1 
Query:  349 MDRTLFEAKLIEELKQHPNLYTKRSQLDFHEYNTIEQSWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKKISCSADIGIDTSQFTKWKHFEKMSFLKNVISHRTINESKSSDQNSMSEQTLKIESTKLESNSISSDPVSDKETFQNDSRSFIDDETNGDISNSFLCKTSNSISVTNFRSPINEEAFGHHIARSLENLSAIRRSVAKVKIQEILSEFEQEDARDPVHLMPNIFLNPFK 1059
            MDRTLFEAKLIEELKQHPNLYTKRSQLDFHEYNTIEQSWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKKISCSADIGIDTSQFTKWKHFEKMSFLKNVISHRTINESKSSDQNSMSEQTLKIES KLESNSISSDPVSDKETFQNDSRSFIDDETNGDISNSFLCKTSNSISVTNFRSPINEEAFGHHIARSLENLSAIRRSVAKVKIQEILSEFEQEDARDPVHLMPNIFLNPFK
Sbjct:    1 MDRTLFEAKLIEELKQHPNLYTKRSQLDFHEYNTIEQSWEIVAKKMDSDTKTCKKIWRNLRDSYVRQLKKISCSADIGIDTSQFTKWKHFEKMSFLKNVISHRTINESKSSDQNSMSEQTLKIESAKLESNSISSDPVSDKETFQNDSRSFIDDETNGDISNSFLCKTSNSISVTNFRSPINEEAFGHHIARSLENLSAIRRSVAKVKIQEILSEFEQEDARDPVHLMPNIFLNPFK 237
The following BLAST results are available for this feature:
BLAST of Transcription factor Adf-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription factor Adf-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
madf-58.040e-630.40MADF domain transcription factor [Source:UniProtK... [more]
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BLAST of Transcription factor Adf-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG77454.961e-829.81gene:FBgn0033616 transcript:FBtr0088119[more]
Adf17.380e-826.96gene:FBgn0284249 transcript:FBtr0330252[more]
Adf18.050e-826.96gene:FBgn0284249 transcript:FBtr0086112[more]
Adf18.050e-826.96gene:FBgn0284249 transcript:FBtr0086111[more]
Adf18.654e-826.96gene:FBgn0284249 transcript:FBtr0086113[more]
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BLAST of Transcription factor Adf-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
si:zfos-128g4.11.209e-620.33si:zfos-128g4.1 [Source:ZFIN;Acc:ZDB-GENE-141212-3... [more]
LO017799.11.299e-628.57transcription factor Adf-1-like [Source:NCBI gene;... [more]
si:ch211-207i20.36.204e-624.19si:ch211-207i20.3 [Source:ZFIN;Acc:ZDB-GENE-141222... [more]
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BLAST of Transcription factor Adf-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription factor Adf-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription factor Adf-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|P05552|ADF1_DROME8.675e-726.96Transcription factor Adf-1 OS=Drosophila melanogas... [more]
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BLAST of Transcription factor Adf-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3R7M2E33.395e-1126.55Transcription factor Adf-1 OS=Penaeus vannamei OX=... [more]
A0A1B0AMX95.077e-934.65MADF domain-containing protein OS=Glossina palpali... [more]
A0A1A9XA615.705e-935.35MADF domain-containing protein OS=Glossina fuscipe... [more]
A0A1A9VUR59.541e-932.33MADF domain-containing protein OS=Glossina austeni... [more]
A0A0A9X9C21.274e-836.27Transcription factor Adf-1 (Fragment) OS=Lygus hes... [more]
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BLAST of Transcription factor Adf-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription factor Adf-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription factor Adf-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription factor Adf-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription factor Adf-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription factor Adf-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000039927.12.263e-17399.58SMESG000039927.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013881 ID=SMED30013881|Name=Transcription factor Adf-1|organism=Schmidtea mediterranea sexual|type=transcript|length=1179bp
TTGTAAACTAAACTTTATTTTCCAAAAAAATTACGTTAATTTTAATTCTA
AGCGTAAAATTAGAATTGATAGGATGTTTTGGTATTTTCTCTACAGTTAT
GTGTGTATTAATGTGTGTTTAACACGTCTGCGAAATCGTACAGGCAGAAC
TGCCTTAACGTGAATGCGTGCGTGAATTAAATCAATTTCGCAGTGTGTGA
AGGAATACAACAATATTGGGTAGGATATTTTTACGTGCAGCAATTCTTGT
TCTCTTACAGTTATCTGTGTGCTCTACGTCAGCGTAGTTAAATTTAGTTG
ATTGAAATAACATTTAGATATTTAGTCATTTTAATGCCGCTTATTCCAAT
GGATCGAACATTATTTGAAGCTAAATTAATAGAGGAATTGAAGCAACATC
CAAATCTGTATACAAAACGTTCACAATTGGATTTTCATGAATATAATACA
ATTGAGCAGTCATGGGAGATAGTGGCAAAAAAGATGGATAGTGATACAAA
GACATGTAAAAAAATATGGAGAAATTTAAGAGATAGTTATGTGAGACAAT
TAAAAAAAATCAGTTGTAGTGCTGACATCGGAATCGATACATCCCAATTC
ACAAAATGGAAACATTTTGAAAAAATGTCATTTTTAAAAAACGTTATTTC
TCATCGAACAATAAATGAAAGTAAATCAAGTGATCAAAATTCAATGAGCG
AACAGACATTAAAAATTGAAAGCACAAAATTGGAATCCAACAGTATTTCT
TCAGATCCAGTATCAGATAAGGAAACTTTTCAAAATGATAGTAGATCTTT
CATTGATGACGAAACTAATGGTGATATTTCAAATTCTTTCTTATGTAAAA
CTTCAAATTCCATCAGTGTCACTAATTTTCGATCTCCTATAAATGAAGAA
GCATTTGGTCATCATATAGCAAGATCTCTTGAAAATCTCAGTGCAATTAG
GCGGTCTGTTGCCAAAGTTAAAATACAAGAAATTCTATCAGAATTTGAGC
AGGAGGATGCTCGTGATCCTGTTCATTTAATGCCGAATATTTTTCTCAAT
CCGTTCAAATAGATTTTTCTTTAATGTAAATCCTCCGTGCAATTCCAATC
TCAACTAGTATGTAGCTTAGAATGACTTATAAATAAAATTTATTTCAATC
TATCCTGTAAAATTCTTGTTTAATATATG
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protein sequence of SMED30013881-orf-1

>SMED30013881-orf-1 ID=SMED30013881-orf-1|Name=SMED30013881-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=243bp
MPLIPMDRTLFEAKLIEELKQHPNLYTKRSQLDFHEYNTIEQSWEIVAKK
MDSDTKTCKKIWRNLRDSYVRQLKKISCSADIGIDTSQFTKWKHFEKMSF
LKNVISHRTINESKSSDQNSMSEQTLKIESTKLESNSISSDPVSDKETFQ
NDSRSFIDDETNGDISNSFLCKTSNSISVTNFRSPINEEAFGHHIARSLE
NLSAIRRSVAKVKIQEILSEFEQEDARDPVHLMPNIFLNPFK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR039353TF_Adf1
IPR006578MADF-dom
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
Vocabulary: biological process
TermDefinition
GO:0006351transcription, DNA-templated
GO:0006355regulation of transcription, DNA-templated
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006578MADF domainSMARTSM00595118neu2coord: 14..106
e-value: 1.6E-17
score: 74.2
IPR006578MADF domainPFAMPF10545MADF_DNA_bdgcoord: 15..101
e-value: 3.6E-17
score: 62.4
IPR006578MADF domainPROSITEPS51029MADFcoord: 14..106
score: 15.339
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..154
IPR039353Transcription factor Adf-1PANTHERPTHR12243FAMILY NOT NAMEDcoord: 14..131