SMED30013870
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30013870 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 21Note: Hover over icons to view figure legend
Alignments
SMED30013870 aligns in the following genomic locations:
Homology
BLAST of SMED30013870 vs. Planmine SMEST
Match: SMESG000014746.1 (SMESG000014746.1) HSP 1 Score: 657.907 bits (1696), Expect = 0.000e+0 Identity = 341/374 (91.18%), Postives = 345/374 (92.25%), Query Frame = 2 Query: 323 QNLRSVYAQCCSLIEQLGGLDTNNMDKRIYFVSSTELFDIRINNNNTCLSNANIKKRHEEFELFEMQLSERIERPRPYKMHFYRNEMKTLNDDLQKIFTKMAKKIGNIDIEASVRPYEDGKMLLENFKTTISKESQXXXXXXXXXXXXXXNKMLNTTSMKDKEGIHKQIADHHSLIGKAIDELLHNLYSEYSWLINNLIGNEQGQEIAVEPLAQFYGMVKAMNAQQGEME-PFISFVRMQSDPPGYRAEIRNSAVEVPVNSDDGNDGQNGTGARPQAEGGIVASIINSPKKIVNLAFGFVRNKANYIIEFTNNKLADLDQKVHKNDAEIINEVTRCISRIENAFNLKVDELITKLNDKINLRKDYKEILSIHFE 1441 QNLRSVYAQCCSLIEQLGGLDTNNMDKRIYFVSSTELFDIRINNNNTCLSNANIKKRHEEFELFEMQLSERIERPRPYKMHFYRNEMKTLNDDLQKIFTKMAKKIGNIDIEASVRPYEDGKMLLENFKTTISKESQKVKLECKEK++CNV KMLNTT+M+DKEGIHKQIADHHSLIGKAIDELLHNLYSEYSWLINNLIGNEQGQEIAVEPLAQFY MVK MNAQQGEME FISFVRMQSDP GYR EIRNSAVEVPVNS GNDGQNGTGARP A GI KIVN FVRN ANYII TN KLADLDQKVHKNDAEIINEVTRCISRIENAFNLKVDELITKLNDKINLRKDYKEILSIHFE Sbjct: 1410 QNLRSVYAQCCSLIEQLGGLDTNNMDKRIYFVSSTELFDIRINNNNTCLSNANIKKRHEEFELFEMQLSERIERPRPYKMHFYRNEMKTLNDDLQKIFTKMAKKIGNIDIEASVRPYEDGKMLLENFKTTISKESQKVKLECKEKIECNVIKMLNTTNMEDKEGIHKQIADHHSLIGKAIDELLHNLYSEYSWLINNLIGNEQGQEIAVEPLAQFYEMVKPMNAQQGEMELQFISFVRMQSDPSGYRVEIRNSAVEVPVNSAGGNDGQNGTGARPNAGWGI---------KIVNSPIEFVRNSANYIIGNTNKKLADLDQKVHKNDAEIINEVTRCISRIENAFNLKVDELITKLNDKINLRKDYKEILSIHFE 1774
BLAST of SMED30013870 vs. Planmine SMEST
Match: SMESG000014746.1 (SMESG000014746.1) HSP 1 Score: 630.172 bits (1624), Expect = 0.000e+0 Identity = 328/361 (90.86%), Postives = 332/361 (91.97%), Query Frame = 2 Query: 323 QNLRSVYAQCCSLIEQLGGLDTNNMDKRIYFVSSTELFDIRINNNNTCLSNANIKKRHEEFELFEMQLSERIERPRPYKMHFYRNEMKTLNDDLQKIFTKMAKKIGNIDIEASVRPYEDGKMLLENFKTTISKESQXXXXXXXXXXXXXXNKMLNTTSMKDKEGIHKQIADHHSLIGKAIDELLHNLYSEYSWLINNLIGNEQGQEIAVEPLAQFYGMVKAMNAQQGEME-PFISFVRMQSDPPGYRAEIRNSAVEVPVNSDDGNDGQNGTGARPQAEGGIVASIINSPKKIVNLAFGFVRNKANYIIEFTNNKLADLDQKVHKNDAEIINEVTRCISRIENAFNLKVDELITKLNDKINL 1402 QNLRSVYAQCCSLIEQLGGLDTNNMDKRIYFVSSTELFDIRINNNNTCLSNANIKKRHEEFELFEMQLSERIERPRPYKMHFYRNEMKTLNDDLQKIFTKMAKKIGNIDIEASVRPYEDGKMLLENFKTTISKESQKVKLECKEK++CNV KMLNTT+M+DKEGIHKQIADHHSLIGKAIDELLHNLYSEYSWLINNLIGNEQGQEIAVEPLAQFY MVK MNAQQGEME FISFVRMQSDP GYR EIRNSAVEVPVNS GNDGQNGTGARP A GI KIVN FVRN ANYII TN KLADLDQKVHKNDAEIINEVTRCISRIENAFNLKVDELITKLNDKINL Sbjct: 1410 QNLRSVYAQCCSLIEQLGGLDTNNMDKRIYFVSSTELFDIRINNNNTCLSNANIKKRHEEFELFEMQLSERIERPRPYKMHFYRNEMKTLNDDLQKIFTKMAKKIGNIDIEASVRPYEDGKMLLENFKTTISKESQKVKLECKEKIECNVIKMLNTTNMEDKEGIHKQIADHHSLIGKAIDELLHNLYSEYSWLINNLIGNEQGQEIAVEPLAQFYEMVKPMNAQQGEMELQFISFVRMQSDPSGYRVEIRNSAVEVPVNSAGGNDGQNGTGARPNAGWGI---------KIVNSPIEFVRNSANYIIGNTNKKLADLDQKVHKNDAEIINEVTRCISRIENAFNLKVDELITKLNDKINL 1761
BLAST of SMED30013870 vs. Planmine SMEST
Match: SMESG000070111.1 (SMESG000070111.1) HSP 1 Score: 115.546 bits (288), Expect = 3.263e-27 Identity = 77/198 (38.89%), Postives = 122/198 (61.62%), Query Frame = 2 Query: 323 QNLRSVYAQCCSLIEQLGGLDTNNMDKRIYFVSSTELFDIRINNNNTCLSNANIKKRHEEFELFEMQLSERIERPRPYKMHFYRNEMKTLNDDLQKIFTKMAKKIGNIDIEASVRPYEDGKMLLENFKTTISKESQXXXXXXXXXXXXXXNKMLNTTSMKDKEGIHKQIADHHSLIGKAIDELLHNLYSEYSWLINNL 916 + +++ +C L+ + GG T ++DKRI F+S+ E+F RI N SN N K R+ +FE FE Q+ E I+ P+ K Y+ E+KTL +L I +++K+I NID E+ V+ YE K FK ++ +K++L+CKE ++C V KMLNT ++ +KE I++QI H+ LI K+I +L L EYS L +++ Sbjct: 261 ETYKNLVGKCFGLVGKFGGPKTKDIDKRIIFISAIEVFGYRIKNKEP--SNRNQKIRYADFERFEDQIEEYIQNPQQLKTRRYKREIKTLLKELNIIIVELSKQINNIDFESCVKRYEGYKTQALIFKEITRRDCEKLQLQCKENIECKVTKMLNTINIDNKE-INEQITQHYDLINKSISDLFQELEREYSKLASDI 455
BLAST of SMED30013870 vs. Planmine SMEST
Match: SMESG000014747.1 (SMESG000014747.1) HSP 1 Score: 109.768 bits (273), Expect = 6.019e-25 Identity = 114/374 (30.48%), Postives = 173/374 (46.26%), Query Frame = 2 Query: 332 RSVYAQCCSLIEQLGGLDTNNMDKRIYFVSSTELFDIRINNNNTCLSNANIKKRHEEFELFEMQLSERIERPRPYKMHFYRNEMKTLNDDLQKIFTKMAKKIGNIDIEASVRPYEDGKMLLENFKTTISKESQXXXXXXXXXXXXXXNKMLNTTSMKDKEGIHKQIADHHSLIGKAIDELLHNLYSEYSWLINNLIGNEQGQEIAVEPLAQFYGMVKAMNAQQGEMEP-----FISFVRMQSDPPGYRAEIRNSAVEVPVNSDDGNDGQNGTGARPQAEGGIVASIINSPKKIVNLAFGFVRNKANYIIEFTNNKLADLDQKVHKNDAEIINEVTRCISRIENAFNLKVDELITKLNDKINLRKDYKEILSIHF 1438 +++ +C +LI+ G T ++DKRIYFVS+ EL DIRIN + L+ + K+R+EEF FE +SE I+ ++ R E+ ++ + + K+ K+I ID ++SV E KM E F I K +K K EC EK+KCNV MLNTT+ DK+ I +IA H+ I ++ L+ LY +Y LI L+G QG EI+ E + + N+ + +++P F+SF+ P G I + IN E + L ++D+ +H ND + V IS I+ F K L+ + N +INLR YK L F Sbjct: 250 KALKGRCQNLIKTFMGKQTKDIDKRIYFVSAKELLDIRINKKD--LNGSKEKERYEEFLHFENYISEYIQNYETSEIC--RTEIDIERNNFKNLVEKLKKRINKIDFDSSVGKLEKDKMQTERFTKEIQKICEKFKSECNEKIKCNVTTMLNTTTF-DKKIIQNKIAKHYFGINRSFRALIKELYEKYGSLI-RLLGEAQGAEISTEQIQ--LNDISQNNSMEVDLDPGVATRFLSFI-------------------------------------PIISGFITNAYINKM----------------LTKEISIEDLNEIDKTIHSNDDGKLKMVDDNISNIKFYFTSKAAALMDRQNREINLRNRYKNYLHDFF 562
BLAST of SMED30013870 vs. Planmine SMEST
Match: SMESG000014753.1 (SMESG000014753.1) HSP 1 Score: 65.4698 bits (158), Expect = 7.343e-11 Identity = 50/187 (26.74%), Postives = 91/187 (48.66%), Query Frame = 2 Query: 338 VYAQCCSLIEQLGGLDTNNMDKRIYFVSSTELFDIRINNNNTCL-SNANIKKRHEEFELFEMQLSERIERPRPYKMHFYRNEMKTLNDDLQKIFTKMAKKIGNIDIEASVRPYEDGKMLLENFKTTISKESQXXXXXXXXXXXXXXNKMLNTTSMKDKEGIHKQIADHHSLIGKAIDELLHNLYSEY 895 + +C + +L + + RIYF S+ E+ IRI NN++ + S+ N+KKR E+F FE L + I P+ K Y E+K + D L F K I N + + YE +E+FK TI ++ V+ ++L+ N+++++ ++ E + Q+ S + + E Y ++ Sbjct: 263 IKEECSKFLRKLEITKEDEIYDRIYFFSAREMSQIRIENNDSLVDSDPNVKKRFEDFMRFEYNLKKYINDPQDNKTRRYMQEIKNIQDFLNTEFMKFQADIFNEKNNSLIPKYEKWMKDMEDFKATILSKNDDVQNSLLKRLEDIHNEIIHSPDLRSSEWVTSQLEHFTSEMKLKLQEAQLKQYKDF 449 The following BLAST results are available for this feature:
BLAST of SMED30013870 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30013870 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30013870 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30013870 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30013870 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30013870 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30013870 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30013870 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30013870 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30013870 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30013870 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30013870 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30013870 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30013870 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30013870 ID=SMED30013870|Name=SMED30013870|organism=Schmidtea mediterranea sexual|type=transcript|length=1810bpback to top protein sequence of SMED30013870-orf-1 >SMED30013870-orf-1 ID=SMED30013870-orf-1|Name=SMED30013870-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=350bp MDKRIYFVSSTELFDIRINNNNTCLSNANIKKRHEEFELFEMQLSERIERback to top Annotated Terms
The following terms have been associated with this transcript:
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