SMED30013851
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30013851 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9
Homology
BLAST of SMED30013851 vs. TrEMBL
Match: R7V1A0 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_200340 PE=4 SV=1) HSP 1 Score: 88.9669 bits (219), Expect = 3.493e-18 Identity = 60/172 (34.88%), Postives = 92/172 (53.49%), Query Frame = 2 Query: 125 DPTRDNFLRLSAEYDKVNDDLKRIQGYIETLEEEYNQCKMMDVFRRYQKLKGMIKRITMHLKMPGMTACAGAKPLGISAIGNLAQGTKDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNVTKSSLI 640 D R N L LSAE +V ++ K ++G +E L EY K +D FRRY LKGMIKR +HLK +T+ P + G K+ E+ RKREE I ++ DE+ +E I++ +K + +++ + D I +M+ YE S+ RY+ +K +IK V + I Sbjct: 17 DVRRVNEL-LSAECSQVQEEYKHLRGVVEQLSREYEDSKEVDFFRRYGMLKGMIKRSVLHLK---LTSNEEHNP-HVPTCG----------KQKEENRKREEHIVGLSKDEITQENDEIQEQIKEYRRKISMMKDLIEQMDHSYEVSKRYIAVQRYRLMKVMIKTVVHNKWI 173
BLAST of SMED30013851 vs. TrEMBL
Match: A0A0R3SJC9 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS5042 PE=4 SV=1) HSP 1 Score: 90.5077 bits (223), Expect = 6.146e-18 Identity = 51/186 (27.42%), Postives = 97/186 (52.15%), Query Frame = 2 Query: 119 LKDPTRDNFLRLSAEYDKVNDDLKRIQGYIETLEEEYNQCK---MMDVFRRYQKLKGMIKRITMHLKMPGMTACAGAKPLGISAIGNLAQGTKDM---------VKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNVTKSSLI 640 + DP + +++LS E D+++ + KR++G+++ EEY K MMD +RRY++L M+K++ +HL++ G S G+ +GT VK E +K T+++L RET+++ + + ++++ + D I ++ Y+E + P LRY LK IK+ ++ I Sbjct: 81 IGDPNKPKYMQLSMENDELHAEWKRLKGFVQNFHEEYKASKGAVMMDPYRRYKRLCAMVKKLVLHLRLNDPAPNLSVTNEGESGRGSFLKGTPSFAGGQVLQSSVKEREMRQKHSNMCDSFTTEQLARETEKLREKNNDLQNRIDLLIDNINELNDSYDECKRSAPLLRYHHLKIAIKSFLANNSI 266
BLAST of SMED30013851 vs. TrEMBL
Match: A0A564ZAE9 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS14077 PE=4 SV=1) HSP 1 Score: 89.3521 bits (220), Expect = 1.519e-17 Identity = 51/186 (27.42%), Postives = 96/186 (51.61%), Query Frame = 2 Query: 119 LKDPTRDNFLRLSAEYDKVNDDLKRIQGYIETLEEEYNQCK---MMDVFRRYQKLKGMIKRITMHLKMPGMTACAGAKPLGISAIGNLAQGTKDM---------VKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNVTKSSLI 640 + DP + +++LS E D+++ + KR++G+++ EEY K MMD +RRY++L M+K++ +HL++ G S G+ +GT VK E +K T+++L RET+++ + ++++ + D I ++ Y+E + P LRY LK IK+ ++ I Sbjct: 81 IGDPNKPKYMQLSMENDELHAEWKRLKGFVQNFHEEYKASKGAVMMDPYRRYKRLCAMVKKLVLHLRLNDPAPNLSVTNEGESGRGSFLKGTPSFAGGQVLQSSVKEREMRQKHSNMCDSFTTEQLARETEKLRGKNNDLQNRIDLLIDNINELNDSYDECKRSAPLLRYHHLKIAIKSFLANNSI 266
BLAST of SMED30013851 vs. TrEMBL
Match: A0A087VX85 (Uncharacterized protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000043700 PE=4 SV=1) HSP 1 Score: 88.1965 bits (217), Expect = 3.509e-17 Identity = 51/182 (28.02%), Postives = 96/182 (52.75%), Query Frame = 2 Query: 119 LKDPTRDNFLRLSAEYDKVNDDLKRIQGYIETLEEEYNQCK---MMDVFRRYQKLKGMIKRITMHLKM---PGMTACAGAKPLGISAIGNLAQGT--KDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNVTKSSLI 640 + DP R +++L+ E D+++ + KR++GY + EEY + K M+D +RRY++L M+K++ +HL++ ++ G + G + + G + VK E +K +T+++L RET+++ D +S++ + D I K+ YE + FLRY LK K +S+ Sbjct: 81 IGDPNRPKYMQLAMENDELHAEWKRLKGYAQNFREEYKESKEAMMLDPYRRYKRLCAMVKKLVLHLRLNDSAPLSGAVGGRGSGPKHGPSFSSGQVLQSYVKEREMRQKHTNVCDSLTTEQLARETEKLRDKNSELQSRIDLLIDGINKLSTAYEMCKRSPFFLRYFHLKVAAKVFIESTCF 262
BLAST of SMED30013851 vs. TrEMBL
Match: A0A0R3T3D8 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS1514 PE=4 SV=2) HSP 1 Score: 87.8113 bits (216), Expect = 5.775e-17 Identity = 52/172 (30.23%), Postives = 93/172 (54.07%), Query Frame = 2 Query: 125 DPTRDNFLRLSAEYDKVNDDLKRIQGYIETLEEEYNQCK---MMDVFRRYQKLKGMIKRITMHLKM----PGMTACAGAKPLGISAI-GNLAQGTKDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIK 616 DP R +++LS E D+++ + KR++ +++ EEY K MMD +RRY++L M+K++ +HL++ P GA S++ G + Q T VK E +K T++EL RET ++ + + ++++ + D I ++ Y++ + P LRY LK IK Sbjct: 82 DPNRPKYMQLSLENDELHAEWKRLRSFVQNFHEEYKASKEALMMDPYRRYKRLCAMVKKLVLHLRLSDPTPSSVNNGGAGAARASSLKGQVLQST---VKEREMRQKHCNMCDSYTTEELARETDKLRERNVDLQNRIDLLIDNINELSDSYDKCKRTAPLLRYHHLKNAIK 250
BLAST of SMED30013851 vs. Planmine SMEST
Match: SMESG000032554.1 (SMESG000032554.1) HSP 1 Score: 402.134 bits (1032), Expect = 1.635e-144 Identity = 195/195 (100.00%), Postives = 195/195 (100.00%), Query Frame = 2 Query: 65 MAGFKSWLKRGKDGSFDILKDPTRDNFLRLSAEYDKVNDDLKRIQGYIETLEEEYNQCKMMDVFRRYQKLKGMIKRITMHLKMPGMTACAGAKPLGISAIGNLAQGTKDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNVTKSSLIRDT 649 MAGFKSWLKRGKDGSFDILKDPTRDNFLRLSAEYDKVNDDLKRIQGYIETLEEEYNQCKMMDVFRRYQKLKGMIKRITMHLKMPGMTACAGAKPLGISAIGNLAQGTKDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNVTKSSLIRDT Sbjct: 1 MAGFKSWLKRGKDGSFDILKDPTRDNFLRLSAEYDKVNDDLKRIQGYIETLEEEYNQCKMMDVFRRYQKLKGMIKRITMHLKMPGMTACAGAKPLGISAIGNLAQGTKDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNVTKSSLIRDT 195
BLAST of SMED30013851 vs. Planmine SMEST
Match: SMESG000032554.1 (SMESG000032554.1) HSP 1 Score: 298.13 bits (762), Expect = 8.108e-104 Identity = 154/195 (78.97%), Postives = 158/195 (81.03%), Query Frame = 2 Query: 65 MAGFKSWLKRGKDGSFDILKDPTRDNFLRLSAEYDKVNDDLKRIQGYIETLEEEYNQCKMMDVFRRYQKLKGMIKRITMHLKMPGMTACAGAKPLGISAIGNLAQGTKDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNVTKSSLIRDT 649 MAGFKSWLKRGKDGSFDILKDPTRDNFLR + ++ K N + MIKRITMHLKMPGMTACAGAKPLGISAIGNLAQGTKDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNVTKSSLIRDT Sbjct: 1 MAGFKSWLKRGKDGSFDILKDPTRDNFLRATLKHWKRN-------------------------------ITRMIKRITMHLKMPGMTACAGAKPLGISAIGNLAQGTKDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNVTKSSLIRDT 164
BLAST of SMED30013851 vs. Planmine SMEST
Match: SMESG000044339.1 (SMESG000044339.1) HSP 1 Score: 180.259 bits (456), Expect = 9.358e-57 Identity = 87/200 (43.50%), Postives = 136/200 (68.00%), Query Frame = 2 Query: 68 AGFKSWLKRGKD---GSFDILKDPTRDNFLRLSAEYDKVNDDLKRIQGYIETLEEEYNQCKMMDVFRRYQKLKGMIKRITMHLKMPGMTACAG---AKPLGISAIGNLAQGTKDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNVTKSSLIRDT 649 AG WLK+ + G+F+++KDP R+++ RL+ EYDK ++ KR++ +++ LE +Y+ K +DV RRY LKGMIKR +HLK+P +T+ +GISA+GNL QGT+ VKR+EQA+K EE+++ + S+E+I E +++E+S+K+ K + A I+DAI +E+ Y S+ P RY ALK+IIK L+RD+ Sbjct: 5 AGVSGWLKKSTNTSLGTFELMKDPVREDYQRLAEEYDKSCEEYKRLRSFVDILEADYSSSKSLDVIRRYLSLKGMIKRTVLHLKLPHITSGKSDLNPNAVGISAMGNLLQGTRTFVKRSEQAKKWEERLQTLKSEEIIAENRKMEESIKDYKRKTAMITDAINNLEVEYTASKIYFPIRRYPALKQIIKRTIHDQLLRDS 204
BLAST of SMED30013851 vs. Planmine SMEST
Match: SMESG000069423.1 (SMESG000069423.1) HSP 1 Score: 91.2781 bits (225), Expect = 6.184e-22 Identity = 50/176 (28.41%), Postives = 98/176 (55.68%), Query Frame = 2 Query: 116 ILKDPTRDNFLRLSAEYDKVNDDLKRIQGYIETLEEEYNQCKMMDVFRRYQKLKGMIKRITMHLKMPGMTA-CAGAKPLGISAIGNLAQGTKDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNVTKSSLI 640 +LKDP ++ F+++ ++D + + KR++ Y+E L+++Y + + RY +LK MIKR MHLK+ + C+ A +K E ARK EE+ + +TSDE+ +E + I+++++ + ++ + AI +E HY +++ + RYQ +K ++K++ I Sbjct: 83 LLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCSNA------------------IKIKELARKSEERYESLTSDEIDKEKQEIKENIEEFQQRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTI 240
BLAST of SMED30013851 vs. Planmine SMEST
Match: SMESG000023281.1 (SMESG000023281.1) HSP 1 Score: 54.6842 bits (130), Expect = 1.011e-8 Identity = 40/146 (27.40%), Postives = 74/146 (50.68%), Query Frame = 2 Query: 188 KRIQGYIETLEEEYNQCKMMDVF-RRYQKLKGMIKRITMHLKMPGMTACAGAKPLGISAIGNLAQGTKDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNV 622 KR++ +I+TL ++Y+ + + + RY+ LK MIKR MHLK +G Q + + K E RK EE+ +K+ S+ LI E +++ ++ +++ A + + ++ Y S+ RYQ L +IK++ Sbjct: 99 KRLKQHIDTLAKDYSCLQTSNNWLSRYRMLKSMIKRCVMHLK-----------------VGEDTQISAGVAK--EINRKCEEKYEKLNSETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIKDL 225 The following BLAST results are available for this feature:
BLAST of SMED30013851 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30013851 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30013851 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30013851 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30013851 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30013851 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30013851 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30013851 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30013851 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30013851 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30013851 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30013851 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30013851 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30013851 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30013851 ID=SMED30013851|Name=SMED30013851|organism=Schmidtea mediterranea sexual|type=transcript|length=734bpback to top protein sequence of SMED30013851-orf-1 >SMED30013851-orf-1 ID=SMED30013851-orf-1|Name=SMED30013851-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=196bp MAGFKSWLKRGKDGSFDILKDPTRDNFLRLSAEYDKVNDDLKRIQGYIETback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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