SMED30013768
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30013768 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Alignments
SMED30013768 aligns in the following genomic locations:
Homology
BLAST of SMED30013768 vs. Planmine SMEST
Match: SMESG000056915.1 (SMESG000056915.1) HSP 1 Score: 164.851 bits (416), Expect = 4.604e-54 Identity = 78/78 (100.00%), Postives = 78/78 (100.00%), Query Frame = 1 Query: 91 MSEKQSSNFPKIWNLFRKHEVAVAYEDVGERKCKTCGQLLVDAYICPFGCFCCSNCISRHMKSERCPPQIAQNNVSNI 324 MSEKQSSNFPKIWNLFRKHEVAVAYEDVGERKCKTCGQLLVDAYICPFGCFCCSNCISRHMKSERCPPQIAQNNVSNI Sbjct: 1 MSEKQSSNFPKIWNLFRKHEVAVAYEDVGERKCKTCGQLLVDAYICPFGCFCCSNCISRHMKSERCPPQIAQNNVSNI 78
BLAST of SMED30013768 vs. Planmine SMEST
Match: SMESG000040257.1 (SMESG000040257.1) HSP 1 Score: 50.447 bits (119), Expect = 7.989e-8 Identity = 23/61 (37.70%), Postives = 33/61 (54.10%), Query Frame = 1 Query: 115 FPKIWNLFRKHEVAVAYEDVGERKCKTCGQLLVDAYICPFGCFCCSNCISRHMKSER--CP 291 F IW + R++E E E KC+ C L+++A P GC CSNCI +H+ + CP Sbjct: 2 FTNIWKMMRENEFESDNEISNELKCRICKCLMINACNAPCGCRFCSNCIEQHLSTNDKFCP 62
BLAST of SMED30013768 vs. Planmine SMEST
Match: SMESG000068193.1 (SMESG000068193.1) HSP 1 Score: 49.6766 bits (117), Expect = 1.118e-7 Identity = 23/63 (36.51%), Postives = 37/63 (58.73%), Query Frame = 1 Query: 109 SNFPKIWNLFRKHEVAVAYEDVGERKCKTCGQLLVDAYICPFGCFCCSNCISRHMKS--ERCP 291 +NF +IWNL K+ + + KC CG+LL+DA+ GC C NCI ++++ ++CP Sbjct: 2 TNFNEIWNLLEKYSFKIDSHLLQHLKCIICGKLLIDAHESLCGCNYCQNCIESYLENGPKQCP 64
BLAST of SMED30013768 vs. Planmine SMEST
Match: SMESG000040257.1 (SMESG000040257.1) HSP 1 Score: 48.9062 bits (115), Expect = 2.395e-7 Identity = 23/61 (37.70%), Postives = 33/61 (54.10%), Query Frame = 1 Query: 115 FPKIWNLFRKHEVAVAYEDVGERKCKTCGQLLVDAYICPFGCFCCSNCISRHMKSER--CP 291 F IW + R++E E E KC+ C L+++A P GC CSNCI +H+ + CP Sbjct: 2 FTNIWKMMRENEFESDNEISNELKCRICKCLMINACNAPCGCRFCSNCIEQHLSTNDKFCP 62
BLAST of SMED30013768 vs. Planmine SMEST
Match: SMESG000065577.1 (SMESG000065577.1) HSP 1 Score: 48.1358 bits (113), Expect = 4.053e-7 Identity = 28/78 (35.90%), Postives = 43/78 (55.13%), Query Frame = 1 Query: 124 IWNLFRKHEVAVAYEDVGERKCKTCGQLLVDAYICPFGCFCCSNCISRHM--KSERCP--PQIAQNNVSNI*IKYSND 345 +W R++E+ ++YE E +CK C +L++ A P GC CS+CI H+ + CP + QN V I Y+ND Sbjct: 5 VWKFMRENEIKISYEVSKELQCKICNKLMIKACHVPCGCRFCSDCIQHHLIGNDKLCPGTSRRCQNRV----INYAND 78 The following BLAST results are available for this feature:
BLAST of SMED30013768 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30013768 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30013768 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30013768 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30013768 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30013768 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30013768 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30013768 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30013768 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30013768 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30013768 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30013768 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30013768 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30013768 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30013768 ID=SMED30013768|Name=SMED30013768|organism=Schmidtea mediterranea sexual|type=transcript|length=425bpback to top Annotated Terms
The following terms have been associated with this transcript:
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