SMED30013750
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30013750 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Alignments
SMED30013750 aligns in the following genomic locations:
Homology
BLAST of SMED30013750 vs. Planmine SMEST
Match: SMESG000016699.1 (SMESG000016699.1) HSP 1 Score: 575.474 bits (1482), Expect = 0.000e+0 Identity = 347/359 (96.66%), Postives = 351/359 (97.77%), Query Frame = 2 Query: 137 HFRNVNEK*LNCLIGNGQKEFKIIGYSDPTKMYRVNSEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSXXXXXXXXXXXXXGYSGYASLSWMSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSEXXXXXXXXXXXXNMIFLVIELIVSFIVILAILRAFQYDXXXXXXXXXXXXXXXXXXSXXXXXXXXXXXECENEFQVIDRIVKELENRGFSSYFYHRDKIYGRDTIEDITQKLKNADLIVIWASENMWENSTCNFCMQIILNRFCNIKRIYIMTSRTKLVLKTCESKQVLIDTKSCLSSKRFRCNLDVISKDQDRTNYSETWYWHYFLSWLISSLPLESVCKDNTL 1213 H N+ ++ +GNGQKEFKIIGYSDPTKMYRVNSEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSNLTTDRTNDTLTNGYSGYASLSWMSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSELLHLFHFRLYRRNMIFLVIELIVSFIVILAILRAFQYDIKIIIFQIIRKFIKQKNISNNYNFLFLYNFECENEFQVIDRIVKELENRGFSSYFYHRDKIYGRDTIEDITQKLKNADLIVIWASENMWENSTCNFCMQIILNRFCNIKRIYIMTSRTKLVLKTCESKQVLIDTKSCLSSKRFRCNLDVISKDQDRTNYSETWYWHYFLSWLISSLPLESVCKDNTL Sbjct: 75 HSNNLISAEISLYVGNGQKEFKIIGYSDPTKMYRVNSEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSNLTTDRTNDTLTNGYSGYASLSWMSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSELLHLFHFRLYRRNMIFLVIELIVSFIVILAILRAFQYDIKIIIFQIIRKFIKQKNISNNYNFLFLYNFECENEFQVIDRIVKELENRGFSSYFYHRDKIYGRDTIEDITQKLKNADLIVIWASENMWENSTCNFCMQIILNRFCNIKRIYIMTSRTKLVLKTCESKQVLIDTKSCLSSKRFRCNLDVISKDQDRTNYSETWYWHYFLSWLISSLPLESVCKDNTL 433
BLAST of SMED30013750 vs. Planmine SMEST
Match: SMESG000016699.1 (SMESG000016699.1) HSP 1 Score: 264.618 bits (675), Expect = 1.800e-86 Identity = 160/172 (93.02%), Postives = 164/172 (95.35%), Query Frame = 2 Query: 137 HFRNVNEK*LNCLIGNGQKEFKIIGYSDPTKMYRVNSEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSXXXXXXXXXXXXXGYSGYASLSWMSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSEXXXXXXXXXXXXNMIFLVIELIVSFIVILAILRAFQYD 652 H N+ ++ +GNGQKEFKIIGYSDPTKMYRVNSEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSNLTTDRTNDTLTNGYSGYASLSWMSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSELLHLFHFRLYRRNMIFLVIELIVSFIVILAILRAFQYD Sbjct: 75 HSNNLISAEISLYVGNGQKEFKIIGYSDPTKMYRVNSEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSNLTTDRTNDTLTNGYSGYASLSWMSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSELLHLFHFRLYRRNMIFLVIELIVSFIVILAILRAFQYD 246
BLAST of SMED30013750 vs. Planmine SMEST
Match: SMESG000016701.1 (SMESG000016701.1) HSP 1 Score: 169.859 bits (429), Expect = 1.117e-47 Identity = 120/336 (35.71%), Postives = 197/336 (58.63%), Query Frame = 2 Query: 191 KEFKIIGYSDPTKMYRVN-SEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSXXXXXXXXXXXXXGYSGYASLSWMSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSEXXXXXXXXXXXXNMIFLVIELIVSFIVILAILRAFQYDXXXXXXXXXXXXXXXXXXSXXXXXXXXXXXECENEFQVIDRIVKELENRGFSSYFYHRDKIYGRDTIEDITQKLKNADLIVIWASENMWENSTCNFCMQIILNRFCNIKRIYIMTSRTKLVLKTCESKQVLIDTKSCLSSKRFRCNLDVISKDQDRTNYSETWYWHYFLSWLISSLPLESV 1195 K+ +I+ SD T++ V S +T +C VK S C F+WNR + SI+A SNLT RT DTL GYSGY+ L ++ + N R + F L + ++ S EG YSC + + E + + +L + I++ ++ FI+I + FQYD KI++ + ++ ++ +YNF FLY+F+ E FQ I ++VK++E+R + YFYHRD+IYG+ E++ +KL+ +D+IV W S+ +W+N +FC+Q+++ + R+YIMTS + ++ C S + D KS + S + N+ ++ +S+ W WH SWLISSLP+ S+ Sbjct: 144 KDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCIQMLIRNYPKKTRLYIMTSNKQNAMENCNSSMISADIKSWILSNIYFWNI-------KKSKFSKLWRWHNLSSWLISSLPVHSL 472
BLAST of SMED30013750 vs. Planmine SMEST
Match: SMESG000016701.1 (SMESG000016701.1) HSP 1 Score: 169.859 bits (429), Expect = 1.149e-47 Identity = 120/336 (35.71%), Postives = 197/336 (58.63%), Query Frame = 2 Query: 191 KEFKIIGYSDPTKMYRVN-SEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSXXXXXXXXXXXXXGYSGYASLSWMSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSEXXXXXXXXXXXXNMIFLVIELIVSFIVILAILRAFQYDXXXXXXXXXXXXXXXXXXSXXXXXXXXXXXECENEFQVIDRIVKELENRGFSSYFYHRDKIYGRDTIEDITQKLKNADLIVIWASENMWENSTCNFCMQIILNRFCNIKRIYIMTSRTKLVLKTCESKQVLIDTKSCLSSKRFRCNLDVISKDQDRTNYSETWYWHYFLSWLISSLPLESV 1195 K+ +I+ SD T++ V S +T +C VK S C F+WNR + SI+A SNLT RT DTL GYSGY+ L ++ + N R + F L + ++ S EG YSC + + E + + +L + I++ ++ FI+I + FQYD KI++ + ++ ++ +YNF FLY+F+ E FQ I ++VK++E+R + YFYHRD+IYG+ E++ +KL+ +D+IV W S+ +W+N +FC+Q+++ + R+YIMTS + ++ C S + D KS + S + N+ ++ +S+ W WH SWLISSLP+ S+ Sbjct: 140 KDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCIQMLIRNYPKKTRLYIMTSNKQNAMENCNSSMISADIKSWILSNIYFWNI-------KKSKFSKLWRWHNLSSWLISSLPVHSL 468
BLAST of SMED30013750 vs. Planmine SMEST
Match: SMESG000016701.1 (SMESG000016701.1) HSP 1 Score: 169.859 bits (429), Expect = 1.650e-47 Identity = 120/336 (35.71%), Postives = 197/336 (58.63%), Query Frame = 2 Query: 191 KEFKIIGYSDPTKMYRVN-SEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSXXXXXXXXXXXXXGYSGYASLSWMSLEKNHRIYNFTLNLKSLNESQEGYYSCHITESSANSEXXXXXXXXXXXXNMIFLVIELIVSFIVILAILRAFQYDXXXXXXXXXXXXXXXXXXSXXXXXXXXXXXECENEFQVIDRIVKELENRGFSSYFYHRDKIYGRDTIEDITQKLKNADLIVIWASENMWENSTCNFCMQIILNRFCNIKRIYIMTSRTKLVLKTCESKQVLIDTKSCLSSKRFRCNLDVISKDQDRTNYSETWYWHYFLSWLISSLPLESV 1195 K+ +I+ SD T++ V S +T +C VK S C F+WNR + SI+A SNLT RT DTL GYSGY+ L ++ + N R + F L + ++ S EG YSC + + E + + +L + I++ ++ FI+I + FQYD KI++ + ++ ++ +YNF FLY+F+ E FQ I ++VK++E+R + YFYHRD+IYG+ E++ +KL+ +D+IV W S+ +W+N +FC+Q+++ + R+YIMTS + ++ C S + D KS + S + N+ ++ +S+ W WH SWLISSLP+ S+ Sbjct: 162 KDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRTSILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCIQMLIRNYPKKTRLYIMTSNKQNAMENCNSSMISADIKSWILSNIYFWNI-------KKSKFSKLWRWHNLSSWLISSLPVHSL 490 The following BLAST results are available for this feature:
BLAST of SMED30013750 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30013750 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30013750 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30013750 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30013750 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30013750 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30013750 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30013750 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30013750 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30013750 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30013750 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30013750 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30013750 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30013750 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30013750 ID=SMED30013750|Name=SMED30013750|organism=Schmidtea mediterranea sexual|type=transcript|length=1253bpback to top protein sequence of SMED30013750-orf-1 >SMED30013750-orf-1 ID=SMED30013750-orf-1|Name=SMED30013750-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=329bp MYRVNSEHTIFCSVKAHKKSFCIFTWNRVIENQNRSIIASSNLTTDRTNDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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