Venom allergen 5

Overview
NameVenom allergen 5
Smed IDSMED30013734
Length (bp)359
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Venom allergen 5 (SMED30013734) t-SNE clustered cells

Violin plots show distribution of expression levels for Venom allergen 5 (SMED30013734) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Venom allergen 5 (SMED30013734) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Venom allergen 5 (SMED30013734) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013734

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
tail regionSMED30013734SMESG000003511.1 SmedASXL_018195SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30013734SMESG000003511.1 Contig32741newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30013734SMESG000003511.1 Contig32741uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Venom allergen 5 vs. Ensembl Human
Match: PI16 (peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGNC:21245])

HSP 1 Score: 51.9878 bits (123), Expect = 2.688e-8
Identity = 29/98 (29.59%), Postives = 51/98 (52.04%), Query Frame = 2 
Query:   44 GDLSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFA--GQKIARGVELWFNQ 331
            G L++++K +++ LHN+YR  V        P AS+M  M W EE A+ A+ YA +C++ H+    R+       G+N+      G  +   +E W ++
Sbjct:   26 GALTDEEKRLMVELHNLYRAQVS-------PTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRR-------GENLFAITDEGMDVPLAMEEWHHE 109
[Close]
BLAST of Venom allergen 5 vs. Ensembl Human
Match: PI16 (peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGNC:21245])

HSP 1 Score: 51.9878 bits (123), Expect = 2.688e-8
Identity = 29/98 (29.59%), Postives = 51/98 (52.04%), Query Frame = 2 
Query:   44 GDLSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFA--GQKIARGVELWFNQ 331
            G L++++K +++ LHN+YR  V        P AS+M  M W EE A+ A+ YA +C++ H+    R+       G+N+      G  +   +E W ++
Sbjct:   26 GALTDEEKRLMVELHNLYRAQVS-------PTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRR-------GENLFAITDEGMDVPLAMEEWHHE 109
[Close]
BLAST of Venom allergen 5 vs. Ensembl Human
Match: PI16 (peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGNC:21245])

HSP 1 Score: 51.9878 bits (123), Expect = 2.688e-8
Identity = 29/98 (29.59%), Postives = 51/98 (52.04%), Query Frame = 2 
Query:   44 GDLSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFA--GQKIARGVELWFNQ 331
            G L++++K +++ LHN+YR  V        P AS+M  M W EE A+ A+ YA +C++ H+    R+       G+N+      G  +   +E W ++
Sbjct:   26 GALTDEEKRLMVELHNLYRAQVS-------PTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRR-------GENLFAITDEGMDVPLAMEEWHHE 109
[Close]
BLAST of Venom allergen 5 vs. Ensembl Human
Match: CRISPLD2 (cysteine rich secretory protein LCCL domain containing 2 [Source:HGNC Symbol;Acc:HGNC:25248])

HSP 1 Score: 49.6766 bits (117), Expect = 1.607e-7
Identity = 31/102 (30.39%), Postives = 49/102 (48.04%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARG--VELWFNQVSGFTF 349
            +  +DK+ IL LHN  R  V        P ASNM+ M W +E    A  +A +CI+ H             +GQN+    G+  + G  V+ W+++V  +T+
Sbjct:   51 IPREDKEEILMLHNKLRGQVQ-------PQASNMEYMTWDDELEKSAAAWASQCIWEHGPTS-----LLVSIGQNLGAHWGRYRSPGFHVQSWYDEVKDYTY 140
[Close]
BLAST of Venom allergen 5 vs. Ensembl Human
Match: CRISPLD2 (cysteine rich secretory protein LCCL domain containing 2 [Source:HGNC Symbol;Acc:HGNC:25248])

HSP 1 Score: 49.6766 bits (117), Expect = 1.631e-7
Identity = 31/102 (30.39%), Postives = 49/102 (48.04%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARG--VELWFNQVSGFTF 349
            +  +DK+ IL LHN  R  V        P ASNM+ M W +E    A  +A +CI+ H             +GQN+    G+  + G  V+ W+++V  +T+
Sbjct:   51 IPREDKEEILMLHNKLRGQVQ-------PQASNMEYMTWDDELEKSAAAWASQCIWEHGPTS-----LLVSIGQNLGAHWGRYRSPGFHVQSWYDEVKDYTY 140
[Close]
BLAST of Venom allergen 5 vs. Ensembl Fly
Match: CG8483 (gene:FBgn0038126 transcript:FBtr0082782)

HSP 1 Score: 61.2326 bits (147), Expect = 6.909e-12
Identity = 37/112 (33.04%), Postives = 60/112 (53.57%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKF---------AGQKIARGVELWFNQVSGFTFENS 358
            ++ +++ IIL  HN  RQ V    +   P A NM+++VW +E A++AQ +AD C F HD    R    F  +GQN+A            G   +R ++ WFN+V  ++F ++
Sbjct:   32 ITAEERSIILQEHNRLRQIVATGRYPGQPGAENMREIVWDDELAARAQKWADNCQFRHDP--HRTINRFT-MGQNLAIIWSTAPLDADDGDFPSR-IQSWFNEVQKYSFGDA 139
[Close]
BLAST of Venom allergen 5 vs. Ensembl Fly
Match: CG9400 (gene:FBgn0030562 transcript:FBtr0073920)

HSP 1 Score: 45.8246 bits (107), Expect = 1.459e-6
Identity = 22/80 (27.50%), Postives = 39/80 (48.75%), Query Frame = 2 
Query:   47 DLSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKF 286
            ++S   + ++L +HN+ R  +          A++M  + W  E    A ++A +C FAHD    R    F++ GQN+  F
Sbjct:   90 EMSERRRQLLLDMHNLARSKIASGNLDGYRSAAHMPLLRWDTELEQMAALHAKRCQFAHDKC--RNTPRFKFSGQNIGYF 167
[Close]
BLAST of Venom allergen 5 vs. Ensembl Fly
Match: CG32679 (gene:FBgn0052679 transcript:FBtr0071469)

HSP 1 Score: 44.669 bits (104), Expect = 3.464e-6
Identity = 36/110 (32.73%), Postives = 45/110 (40.91%), Query Frame = 2 
Query:   38 CSGDLSNDDKDI--ILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQN----------VAKFAGQKIARGVELWFNQ 331
            CSG+    D  I  IL LHN  R  +     S  P A  M  M W    A  A   A +C  AHD  + R    + + GQN          VA F  Q+IA     WF++
Sbjct:   50 CSGEFVQVDAHIPLILQLHNERRNLIAGGGVSGFPSAVQMATMSWDTTLAQLAAYNALQCRMAHD--ECRNTNTYRYAGQNLSILFTRSVDVAVFLRQRIA----AWFDE 153
[Close]
BLAST of Venom allergen 5 vs. Ensembl Fly
Match: Ag5r (gene:FBgn0015010 transcript:FBtr0333658)

HSP 1 Score: 44.2838 bits (103), Expect = 4.578e-6
Identity = 25/102 (24.51%), Postives = 48/102 (47.06%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFA-------GQKIARGVELWFNQV 334
            LS+ +KD ++   N YR ++   + +++  A  M  + W++E A  A +    C   HD   +     F+W GQN+A             +  GV++W+++ 
Sbjct:   54 LSSAEKDALVARTNEYRNHIAGGLNANLSAACRMATIKWNDELAYLASLNVKSCQMKHDGCHNTD--AFDWSGQNLAWMGYYNPLNVTHYLEWGVDMWYDEA 153
[Close]
BLAST of Venom allergen 5 vs. Ensembl Fly
Match: Ag5r (gene:FBgn0015010 transcript:FBtr0073937)

HSP 1 Score: 44.2838 bits (103), Expect = 4.578e-6
Identity = 25/102 (24.51%), Postives = 48/102 (47.06%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFA-------GQKIARGVELWFNQV 334
            LS+ +KD ++   N YR ++   + +++  A  M  + W++E A  A +    C   HD   +     F+W GQN+A             +  GV++W+++ 
Sbjct:   54 LSSAEKDALVARTNEYRNHIAGGLNANLSAACRMATIKWNDELAYLASLNVKSCQMKHDGCHNTD--AFDWSGQNLAWMGYYNPLNVTHYLEWGVDMWYDEA 153
[Close]
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: crispld2 (cysteine-rich secretory protein LCCL domain containing 2 [Source:ZFIN;Acc:ZDB-GENE-130131-1])

HSP 1 Score: 50.0618 bits (118), Expect = 8.505e-8
Identity = 32/98 (32.65%), Postives = 51/98 (52.04%), Query Frame = 2 
Query:   62 DKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAG--QKIARGVELWFNQVSGFTF 349
            D++ IL LHN  R     EV+   P ASNM+ M+W +E    A  +A++C + H   D         +GQN+A   G  +  A  V+ W+++V  +T+
Sbjct:   64 DREEILKLHNKLRG----EVY---PTASNMEYMIWDDELERSATSWAEQCQWEHGPQD-----LLMSIGQNLAVHWGRYRSPAYHVQAWYDEVKDYTY 149
[Close]
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: r3hdml (R3H domain containing-like [Source:ZFIN;Acc:ZDB-GENE-090313-275])

HSP 1 Score: 46.9802 bits (110), Expect = 7.299e-7
Identity = 29/104 (27.88%), Postives = 51/104 (49.04%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQ--KIARGVELWFNQVSGFTFEN 355
            +S  D   +L  HN  R  V        P A+NM+ MVW E  A  A+ +A +CI+ H       +   + +GQN++  +G+   I   V+ W+++   F++ +
Sbjct:   61 ISGKDMTALLDYHNRVRSQV-------FPPAANMEYMVWDERLAKSAEFWASQCIWEHGP-----HHFLQHIGQNLSIISGRYKSIIDLVKSWYDERHSFSYPS 152
[Close]
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: r3hdml (R3H domain containing-like [Source:ZFIN;Acc:ZDB-GENE-090313-275])

HSP 1 Score: 46.9802 bits (110), Expect = 7.299e-7
Identity = 29/104 (27.88%), Postives = 51/104 (49.04%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQ--KIARGVELWFNQVSGFTFEN 355
            +S  D   +L  HN  R  V        P A+NM+ MVW E  A  A+ +A +CI+ H       +   + +GQN++  +G+   I   V+ W+++   F++ +
Sbjct:   61 ISGKDMTALLDYHNRVRSQV-------FPPAANMEYMVWDERLAKSAEFWASQCIWEHGP-----HHFLQHIGQNLSIISGRYKSIIDLVKSWYDERHSFSYPS 152
[Close]
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: crispld1a (cysteine-rich secretory protein LCCL domain containing 1a [Source:ZFIN;Acc:ZDB-GENE-090612-1])

HSP 1 Score: 46.9802 bits (110), Expect = 7.922e-7
Identity = 30/102 (29.41%), Postives = 47/102 (46.08%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAG--QKIARGVELWFNQVSGFTF 349
            ++  D   IL LHN  R  V        P ASNM+ MVW  E    A+ +A+ C++ H        G    +GQN+    G  +     V+ W+++V  ++F
Sbjct:   62 ITASDMQAILDLHNKLRGQVY-------PPASNMEYMVWDNELERSAEEWAETCLWEHGPA-----GLLPQIGQNLGVHWGRYRPPTSHVQAWYDEVKDYSF 151
[Close]
BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Match: crispld1a (cysteine-rich secretory protein LCCL domain containing 1a [Source:ZFIN;Acc:ZDB-GENE-090612-1])

HSP 1 Score: 46.9802 bits (110), Expect = 8.040e-7
Identity = 30/102 (29.41%), Postives = 47/102 (46.08%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAG--QKIARGVELWFNQVSGFTF 349
            ++  D   IL LHN  R  V        P ASNM+ MVW  E    A+ +A+ C++ H        G    +GQN+    G  +     V+ W+++V  ++F
Sbjct:   62 ITASDMQAILDLHNKLRGQVY-------PPASNMEYMVWDNELERSAEEWAETCLWEHGPA-----GLLPQIGQNLGVHWGRYRPPTSHVQAWYDEVKDYSF 151
[Close]
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: crispld2 (cysteine-rich secretory protein LCCL domain containing 2 [Source:NCBI gene;Acc:613091])

HSP 1 Score: 50.447 bits (119), Expect = 8.821e-8
Identity = 31/98 (31.63%), Postives = 50/98 (51.02%), Query Frame = 2 
Query:   62 DKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAG--QKIARGVELWFNQVSGFTF 349
            DK+ I+ LHN  R  V        P ASNM+ M W +E    A+ +A++CI+ H             +GQN+A   G  ++ A  V+ W+++V  +T+
Sbjct:   56 DKEEIIQLHNKLRGQVH-------PSASNMEYMTWDDELEKSAEAWAEECIWEHGPT-----ALLMSIGQNLAVHWGRYRQPAYHVQSWYDEVKDYTY 141
[Close]
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: crispld2 (cysteine-rich secretory protein LCCL domain containing 2 [Source:NCBI gene;Acc:613091])

HSP 1 Score: 50.447 bits (119), Expect = 8.859e-8
Identity = 31/98 (31.63%), Postives = 50/98 (51.02%), Query Frame = 2 
Query:   62 DKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAG--QKIARGVELWFNQVSGFTF 349
            DK+ I+ LHN  R  V        P ASNM+ M W +E    A+ +A++CI+ H             +GQN+A   G  ++ A  V+ W+++V  +T+
Sbjct:   56 DKEEIIQLHNKLRGQVH-------PSASNMEYMTWDDELEKSAEAWAEECIWEHGPT-----ALLMSIGQNLAVHWGRYRQPAYHVQSWYDEVKDYTY 141
[Close]
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: crispld2 (cysteine-rich secretory protein LCCL domain containing 2 [Source:NCBI gene;Acc:613091])

HSP 1 Score: 50.447 bits (119), Expect = 8.867e-8
Identity = 31/98 (31.63%), Postives = 50/98 (51.02%), Query Frame = 2 
Query:   62 DKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAG--QKIARGVELWFNQVSGFTF 349
            DK+ I+ LHN  R  V        P ASNM+ M W +E    A+ +A++CI+ H             +GQN+A   G  ++ A  V+ W+++V  +T+
Sbjct:   56 DKEEIIQLHNKLRGQVH-------PSASNMEYMTWDDELEKSAEAWAEECIWEHGPT-----ALLMSIGQNLAVHWGRYRQPAYHVQSWYDEVKDYTY 141
[Close]
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: CRISPLD1 (cysteine-rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:100490431])

HSP 1 Score: 48.521 bits (114), Expect = 3.522e-7
Identity = 31/102 (30.39%), Postives = 50/102 (49.02%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAG--QKIARGVELWFNQVSGFTF 349
            ++  D  +IL LHN  R     EV+   P ASNM+ M+W  E    A+ +A+ C++ H   D         +GQN+    G  +     V+ W+++V  +TF
Sbjct:   57 ITESDMKLILDLHNKLRG----EVY---PPASNMEFMIWDVELERSAEAWAETCLWEHGPAD-----LLPVIGQNLGAHWGRYRPPTYHVQAWYDEVRDYTF 146
[Close]
BLAST of Venom allergen 5 vs. Ensembl Xenopus
Match: CRISPLD1 (cysteine-rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:100490431])

HSP 1 Score: 48.521 bits (114), Expect = 3.606e-7
Identity = 31/102 (30.39%), Postives = 50/102 (49.02%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAG--QKIARGVELWFNQVSGFTF 349
            ++  D  +IL LHN  R     EV+   P ASNM+ M+W  E    A+ +A+ C++ H   D         +GQN+    G  +     V+ W+++V  +TF
Sbjct:   57 ITESDMKLILDLHNKLRG----EVY---PPASNMEFMIWDVELERSAEAWAETCLWEHGPAD-----LLPVIGQNLGAHWGRYRPPTYHVQAWYDEVRDYTF 146
[Close]
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: Crispld2 (cysteine-rich secretory protein LCCL domain containing 2 [Source:MGI Symbol;Acc:MGI:1926142])

HSP 1 Score: 52.373 bits (124), Expect = 1.115e-8
Identity = 32/98 (32.65%), Postives = 48/98 (48.98%), Query Frame = 2 
Query:   62 DKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARG--VELWFNQVSGFTF 349
            D+  IL LHN  R  V        P ASNM+ M W EE    A  +A +C++ H        G    +GQN+A   G+  + G  V+ W+++V  +T+
Sbjct:   55 DRQEILMLHNKLRGQVY-------PPASNMEHMTWDEELERSAAAWAHRCLWEHGPA-----GLLRSIGQNLAVHWGRYRSPGFHVQSWYDEVKDYTY 140
[Close]
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: Crispld2 (cysteine-rich secretory protein LCCL domain containing 2 [Source:MGI Symbol;Acc:MGI:1926142])

HSP 1 Score: 52.373 bits (124), Expect = 1.115e-8
Identity = 32/98 (32.65%), Postives = 48/98 (48.98%), Query Frame = 2 
Query:   62 DKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARG--VELWFNQVSGFTF 349
            D+  IL LHN  R  V        P ASNM+ M W EE    A  +A +C++ H        G    +GQN+A   G+  + G  V+ W+++V  +T+
Sbjct:   55 DRQEILMLHNKLRGQVY-------PPASNMEHMTWDEELERSAAAWAHRCLWEHGPA-----GLLRSIGQNLAVHWGRYRSPGFHVQSWYDEVKDYTY 140
[Close]
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: Crispld2 (cysteine-rich secretory protein LCCL domain containing 2 [Source:MGI Symbol;Acc:MGI:1926142])

HSP 1 Score: 52.373 bits (124), Expect = 1.115e-8
Identity = 32/98 (32.65%), Postives = 48/98 (48.98%), Query Frame = 2 
Query:   62 DKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARG--VELWFNQVSGFTF 349
            D+  IL LHN  R  V        P ASNM+ M W EE    A  +A +C++ H        G    +GQN+A   G+  + G  V+ W+++V  +T+
Sbjct:   55 DRQEILMLHNKLRGQVY-------PPASNMEHMTWDEELERSAAAWAHRCLWEHGPA-----GLLRSIGQNLAVHWGRYRSPGFHVQSWYDEVKDYTY 140
[Close]
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: Pi16 (peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:1921366])

HSP 1 Score: 51.2174 bits (121), Expect = 3.503e-8
Identity = 25/65 (38.46%), Postives = 37/65 (56.92%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRK 244
            L+ D+K  ++ LHN YR  V        P AS+M QM W +E A+ A+ YA KC++ H+    R+
Sbjct:   30 LTEDEKQTMVDLHNQYRAQVS-------PPASDMLQMRWDDELAAFAKAYAQKCVWGHNKERGRR 87
[Close]
BLAST of Venom allergen 5 vs. Ensembl Mouse
Match: Pi16 (peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:1921366])

HSP 1 Score: 50.447 bits (119), Expect = 4.021e-8
Identity = 25/65 (38.46%), Postives = 37/65 (56.92%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRK 244
            L+ D+K  ++ LHN YR  V        P AS+M QM W +E A+ A+ YA KC++ H+    R+
Sbjct:   30 LTEDEKQTMVDLHNQYRAQVS-------PPASDMLQMRWDDELAAFAKAYAQKCVWGHNKERGRR 87
[Close]
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|W4VS53|CRVP_TRILK (CRISP/Allergen/PR-1 OS=Trittame loki OX=1295018 PE=1 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 3.827e-15
Identity = 44/106 (41.51%), Postives = 60/106 (56.60%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVY--EVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNV------AKFAGQKIARGVELWFNQVSGF 343
            +S+ DK+IIL  HN  R  V    E    +P AS+M Q+VW +E AS AQ +AD+C+F HD  D R   +F  VGQN+      AKF+    A  V  W+++V  F
Sbjct:   49 VSSGDKEIILREHNKLRSRVATGKETKYSLPKASDMMQLVWDDELASVAQKHADQCVFEHDCNDCRNVQNFG-VGQNLHLRTSSAKFSDITWAGAVNGWYDEVKDF 153
[Close]
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|P0DPV2|VA558_SCODE (Scoloptoxin SSD558 OS=Scolopendra dehaani OX=2609776 PE=1 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 3.375e-13
Identity = 34/95 (35.79%), Postives = 53/95 (55.79%), Query Frame = 2 
Query:   65 KDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFA-GQKIARGVELWFNQVSGFT 346
            K +IL LHN  RQ V     S  P A+NM+++ W++E A+ AQ  A+ C+F H     RK   + ++G+N+ K +    I R V  W+++V   T
Sbjct:   36 KKMILDLHNKKRQIVANGQQSGQPSAANMKELHWNDEIAANAQRSAETCVFEHTAKSLRKTTKYSYLGENIYKGSYPDPIPRSVNAWYDEVKDVT 130
[Close]
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|P0DPU1|VA576_SCODE (Scoloptoxin SSD976 OS=Scolopendra dehaani OX=2609776 PE=1 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 3.670e-13
Identity = 34/95 (35.79%), Postives = 53/95 (55.79%), Query Frame = 2 
Query:   65 KDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFA-GQKIARGVELWFNQVSGFT 346
            K +IL LHN  RQ V     S  P A+NM+++ W++E A+ AQ  A+ C+F H     RK   + ++G+N+ K +    I R V  W+++V   T
Sbjct:   36 KKMILDLHNKKRQIVANGQQSGQPSAANMKELHWNDEIAANAQRSAETCVFEHTAKSLRKTTKYSYLGENIYKGSYPDPIPRSVNAWYDEVKDVT 130
[Close]
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|P0DPU0|VA552_SCODE (Scoloptoxin SSD552 OS=Scolopendra dehaani OX=2609776 PE=1 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 3.286e-12
Identity = 34/95 (35.79%), Postives = 51/95 (53.68%), Query Frame = 2 
Query:   65 KDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFA-GQKIARGVELWFNQVSGFT 346
            K +IL LHN  RQ V     S  P+A+NM+++ W +E  + AQ  A+ C+F H     RK   + ++G+NV K      I R V  W+++V   T
Sbjct:   36 KKMILDLHNKKRQIVANGQQSGQPLAANMKELHWDDEIEAIAQRSAETCVFKHTAKSLRKSSKYSYLGENVYKGGYPDPIPRSVNKWYDEVKDVT 130
[Close]
BLAST of Venom allergen 5 vs. UniProt/SwissProt
Match: sp|P85840|VA5_TITSE (Venom allergen 5 (Fragments) OS=Tityus serrulatus OX=6887 PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.184e-10
Identity = 31/78 (39.74%), Postives = 46/78 (58.97%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVY--EVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNV 277
            +S DD++ I+ LHN  R  +    +    +P A +M +M W +E A  AQ  AD+C+F HD  D RK  +F+ VGQN+
Sbjct:   30 VSQDDRNTIINLHNKVRNNIALGQDQSGRLPAAGDMLEMEWDDELAQIAQKLADQCVFKHDCDDCRKVENFD-VGQNI 106
[Close]
BLAST of Venom allergen 5 vs. TrEMBL
Match: I1ZI85 (GLI pathogenesis related-1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 8.877e-57
Identity = 86/115 (74.78%), Postives = 96/115 (83.48%), Query Frame = 2 
Query:   14 FVLVVLEFCSGDLSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARGVELWFNQVSGFTFENS 358
            FVLVVL+FCSGDLS  +K ++L LHN +R  +     S  P A+NMQQMVW+ ESASKAQI ADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQ I+RGV LW+NQVSGFTFENS
Sbjct:    3 FVLVVLDFCSGDLSEKEKKLLLALHNGFRSNLAQGEVSGQPPATNMQQMVWNAESASKAQITADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQDISRGVTLWYNQVSGFTFENS 117
[Close]
BLAST of Venom allergen 5 vs. TrEMBL
Match: A0A1C9ZZV5 (SCP domain-containing protein (Fragment) OS=Dugesia japonica OX=6161 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.031e-15
Identity = 42/115 (36.52%), Postives = 65/115 (56.52%), Query Frame = 2 
Query:    8 VGFVLVVLEFCSGDLSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARGVELWFNQVSGFTFE 352
            + F+ + L FC+     + KD +L  HN  R+ +      + P A+NM +MVW +  A KA+ +A KCI  HDT DDR+   F +VGQN+  FAG      V+ W+++   +T+E
Sbjct:   15 ISFISIALIFCT---PPEYKDFLLKAHNDLRRDIALGKTPNQPAAANMIEMVWDDALAGKAEEWAGKCIAGHDTYDDRQLDKFMFVGQNM--FAGSNYKEVVQGWYDEYKDYTYE 124
[Close]
BLAST of Venom allergen 5 vs. TrEMBL
Match: A0A482XQ64 (SCP domain-containing protein OS=Laodelphax striatellus OX=195883 GN=LSTR_LSTR008730 PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.100e-15
Identity = 47/114 (41.23%), Postives = 63/114 (55.26%), Query Frame = 2 
Query:   35 FCSGDLSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKF---AGQKIARG------VELWFNQVSGFTF 349
            F SG LS  +KD I++LHN  RQ V     S  P A+NM +M W EE A KAQ +AD C   HD+   R+ G F  VGQN+AK    +G+  A        +  WF++V  ++F
Sbjct:   25 FESGGLSCQEKDSIVSLHNTLRQRVALGSVSGQPPAANMLEMAWDEELARKAQSWADSCPAGHDSNYARRVGRFS-VGQNIAKTWSSSGRSYAESPNFSSQINAWFSEVYHYSF 137
[Close]
BLAST of Venom allergen 5 vs. TrEMBL
Match: A0A0R3UG82 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS6212 PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.882e-15
Identity = 36/99 (36.36%), Postives = 60/99 (60.61%), Query Frame = 2 
Query:   62 DKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARGVELWFNQVSGFTFENS 358
            +K  +L LHN YR+ V      + P +S +  + WS E  + AQ +AD C FAHD+ D R+   ++WVGQN+A  +   IA+ VE+WF++   + ++++
Sbjct:   56 EKSELLNLHNKYRRLVSDGQIREQPSSSKIHDLKWSTELEASAQRHADTCRFAHDSADARRTPQWDWVGQNIAYSSS--IAKNVEMWFDEYKEYNYQSN 152
[Close]
BLAST of Venom allergen 5 vs. TrEMBL
Match: A0A4E0R0B3 (Pathogenesis protein PRMS OS=Fasciola hepatica OX=6192 GN=D915_009074 PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.659e-15
Identity = 46/119 (38.66%), Postives = 68/119 (57.14%), Query Frame = 2 
Query:   17 VLVVLEFCSGD---LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQK-IARGVELWFNQVSGFTF-ENS 358
            ++V+L F +     +S + K+++L LHN YR+ V        P A  M +M W  E A+KAQ ++D+C   HD ++DRK   F WVGQN   FAG   + RG   WF++ + + F ENS
Sbjct:    7 LIVILGFLATSVVSISQNYKELLLFLHNTYREKVKNCGVPGQPGALTMPRMQWHNELAAKAQKWSDQCKVGHDRLEDRKVNGFWWVGQN---FAGNPSVKRGFFSWFDEHNKYNFTENS 122
[Close]
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: ENSAMXT00000032496.1 (pep primary_assembly:Astyanax_mexicanus-2.0:18:32321795:32342374:-1 gene:ENSAMXG00000031919.1 transcript:ENSAMXT00000032496.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.0694 bits (131), Expect = 8.345e-10
Identity = 32/97 (32.99%), Postives = 51/97 (52.58%), Query Frame = 2 
Query:   65 KDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQ-KIARGVELWFNQVSGFTFE 352
            +D I+ LHN++R+ V        P A+NM +M WS E+A  AQ +A+ C   H     R  G ++ +G+N+ K  G  K    V  W N+V+ + + 
Sbjct:   43 QDEIVNLHNVFRRGVS-------PSATNMLKMNWSREAAVSAQNWANTCSMVHGDPSTRMLGTYQ-MGENLFKSTGMVKWTSVVTAWHNEVANYVYP 131
[Close]
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: crispld2 (cysteine-rich secretory protein LCCL domain containing 2 [Source:ZFIN;Acc:ZDB-GENE-130131-1])

HSP 1 Score: 50.8322 bits (120), Expect = 2.996e-8
Identity = 32/98 (32.65%), Postives = 50/98 (51.02%), Query Frame = 2 
Query:   62 DKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAG--QKIARGVELWFNQVSGFTF 349
            D++ IL LHN  R+ V        P ASNM+ MVW +E    A  +A++C + H   D         +GQN+A   G  +  A  V+ W+++V  +T+
Sbjct:   60 DREEILALHNKLREGVY-------PTASNMEYMVWDDELERSATHWAEQCQWEHGPQD-----LLMSIGQNLAVHWGRYRSPAYHVQAWYDEVKDYTY 145
[Close]
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: crispld1a (cysteine-rich secretory protein LCCL domain containing 1a [Source:ZFIN;Acc:ZDB-GENE-090612-1])

HSP 1 Score: 50.0618 bits (118), Expect = 5.557e-8
Identity = 30/103 (29.13%), Postives = 49/103 (47.57%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKI---ARGVELWFNQVSGFTF 349
            +S+ D   IL LHN  R  V        P +SNM+ MVW  E    A+ +A+ C++ H        G    +GQN+    G+ +      V+ W+++V  ++F
Sbjct:   57 ISSSDMQAILDLHNKLRAQVY-------PPSSNMEYMVWDTELERSAEEWAETCLWEHGPA-----GLLPQIGQNLGVHWGRSVPAPTSHVQAWYDEVKDYSF 147
[Close]
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: r3hdml (R3H domain containing like [Source:NCBI gene;Acc:103046222])

HSP 1 Score: 48.9062 bits (115), Expect = 1.411e-7
Identity = 31/104 (29.81%), Postives = 51/104 (49.04%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAG--QKIARGVELWFNQVSGFTFEN 355
            +S+ D   +L  HN  R  V        P A+NM+ MVW E  A  A+ +A +CI+ H      KY     +GQN++  +G  + +   V  W ++ + F++ N
Sbjct:   62 VSSRDMTALLEYHNRVRSQV-------FPPAANMEYMVWDERLAKSAESWASQCIWDHGPSHVMKY-----MGQNLSISSGRYRSVIELVRSWHDEKNSFSYPN 153
[Close]
BLAST of Venom allergen 5 vs. Ensembl Cavefish
Match: pi15a (peptidase inhibitor 15-A-like [Source:NCBI gene;Acc:103031459])

HSP 1 Score: 48.521 bits (114), Expect = 1.922e-7
Identity = 30/103 (29.13%), Postives = 53/103 (51.46%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAG--QKIARGVELWFNQVSGFTFE 352
            +S +D   IL  HN  R  V        P ASNM+ MVW +  A +A+ +A  C++ H   +  ++     +GQN++   G  + I + V+ W+++V  ++F 
Sbjct:   63 ISQEDMLAILQYHNKVRGKV-------FPPASNMEYMVWDDSLAKRAEEWASTCLWEHGPRNLLRF-----LGQNLSVRTGRYRSILQLVKPWYDEVKDYSFP 153
[Close]
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: ENSORLT00000020674.2 (cysteine-rich venom protein pseudecin-like [Source:NCBI gene;Acc:101157728])

HSP 1 Score: 50.447 bits (119), Expect = 2.998e-8
Identity = 31/109 (28.44%), Postives = 53/109 (48.62%), Query Frame = 2 
Query:   44 GDLSNDDKDI---ILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARG-VELWFNQVSGFTFENS 358
             D+  +D  +   I+ +HN +R+ V        P A++M  M +SEE A  AQ + DKC+  H     R    +E +G+N+       + +  +  W+ +VS F F N+
Sbjct:   21 ADICPEDTAVQAEIVDVHNAFRRAVE-------PPAADMLMMSYSEELAVSAQAWVDKCVLDHGPPSSRMLNGYE-LGENLFYSTSPMLWKSIINAWYKEVSHFAFPNA 121
[Close]
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: ENSORLT00000020676.2 (cysteine-rich venom protein pseudecin-like [Source:NCBI gene;Acc:101157728])

HSP 1 Score: 50.0618 bits (118), Expect = 4.504e-8
Identity = 31/108 (28.70%), Postives = 53/108 (49.07%), Query Frame = 2 
Query:   47 DLSNDDKDI---ILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARG-VELWFNQVSGFTFENS 358
            D+  +D  +   I+ +HN +R+ V        P A++M  M +SEE A  AQ + DKC+  H     R    +E +G+N+       + +  +  W+ +VS F F N+
Sbjct:    7 DICPEDTAVQAEIVDVHNAFRRAVE-------PPAADMLMMSYSEELAVSAQAWVDKCVLDHGPPSSRMLNGYE-LGENLFYSTSPMLWKSIINAWYKEVSHFAFPNA 106
[Close]
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: crispld2 (cysteine rich secretory protein LCCL domain containing 2 [Source:NCBI gene;Acc:101174090])

HSP 1 Score: 48.521 bits (114), Expect = 1.972e-7
Identity = 31/98 (31.63%), Postives = 49/98 (50.00%), Query Frame = 2 
Query:   62 DKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAG--QKIARGVELWFNQVSGFTF 349
            D++ IL LHN  R  V        P A+NM+ MVW +E    A  +A++C + H   D         +GQN+A   G  +  A  V+ W+++V  +T+
Sbjct:   75 DREEILLLHNKLRSSVY-------PSAANMEHMVWDDELERSATQWAEECRWEHGPQD-----LLMSIGQNLAVHWGRYRSPAFHVQAWYDEVKDYTY 160
[Close]
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: crispld1a (cysteine rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:101172850])

HSP 1 Score: 47.3654 bits (111), Expect = 5.639e-7
Identity = 29/102 (28.43%), Postives = 48/102 (47.06%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAG--QKIARGVELWFNQVSGFTF 349
            +++ D  +IL LHN  R  V        P ASNM+ M+W  E    A+ +A+ C++ H             +GQN+    G  Q     V+ W+++V  ++F
Sbjct:   56 ITDSDAQLILDLHNKLRGQVY-------PPASNMEYMMWDTELERSAEEWAETCLWEHGPAS-----LLPQIGQNLGAHWGRYQPPTFHVQAWYDEVKDYSF 145
[Close]
BLAST of Venom allergen 5 vs. Ensembl Medaka
Match: crispld1a (cysteine rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:101172850])

HSP 1 Score: 47.3654 bits (111), Expect = 5.679e-7
Identity = 29/102 (28.43%), Postives = 48/102 (47.06%), Query Frame = 2 
Query:   50 LSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAG--QKIARGVELWFNQVSGFTF 349
            +++ D  +IL LHN  R  V        P ASNM+ M+W  E    A+ +A+ C++ H             +GQN+    G  Q     V+ W+++V  ++F
Sbjct:   84 ITDSDAQLILDLHNKLRGQVY-------PPASNMEYMMWDTELERSAEEWAETCLWEHGPAS-----LLPQIGQNLGAHWGRYQPPTFHVQAWYDEVKDYSF 173
[Close]
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000017386.1 (SMESG000017386.1)

HSP 1 Score: 186.808 bits (473), Expect = 1.617e-61
Identity = 91/119 (76.47%), Postives = 99/119 (83.19%), Query Frame = 2 
Query:    2 IKVGFVLVVLEFCSGDLSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARGVELWFNQVSGFTFENS 358
            I+V FVLVVL+FCSGDLS  +K+++L +HN  R  V     S  P ASNMQQMVW+ ESASKAQI ADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQ IARG ELWFNQVSGFTFENS
Sbjct:    5 IEVAFVLVVLDFCSGDLSEKEKNLLLDVHNERRLAVSIGYASGQPSASNMQQMVWNAESASKAQITADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQDIARGAELWFNQVSGFTFENS 123
[Close]
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000017383.1 (SMESG000017383.1)

HSP 1 Score: 184.882 bits (468), Expect = 4.785e-61
Identity = 86/119 (72.27%), Postives = 101/119 (84.87%), Query Frame = 2 
Query:    2 IKVGFVLVVLEFCSGDLSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARGVELWFNQVSGFTFENS 358
            IK+ FVLVVL+FCSGDLS  +K+++L +HN YR  V    F   P+A+NMQQMVW+EESASKAQIYADKCIFAHDTID+RKYGDFEWVGQNVAKFAG+ I+RG  LW+N+ SGFTFENS
Sbjct:    5 IKIAFVLVVLDFCSGDLSEKEKNLLLDVHNKYRSAVAIGEFPGQPLATNMQQMVWNEESASKAQIYADKCIFAHDTIDNRKYGDFEWVGQNVAKFAGEIISRGDILWYNEASGFTFENS 123
[Close]
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000017389.1 (SMESG000017389.1)

HSP 1 Score: 183.726 bits (465), Expect = 2.828e-60
Identity = 88/119 (73.95%), Postives = 98/119 (82.35%), Query Frame = 2 
Query:    2 IKVGFVLVVLEFCSGDLSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARGVELWFNQVSGFTFENS 358
            IK+ FVLVVL+FCSGDLS  +K+++L +HN YR  V     S  P ASNMQ MVW+E+SASKAQIYADKCIFAHDT DDRKYGDFEWVGQNVAKFAG+ I RG ELWF QVSGFTFENS
Sbjct:    5 IKIAFVLVVLDFCSGDLSEKEKNLLLNIHNEYRGDVAIGQVSGQPTASNMQLMVWNEKSASKAQIYADKCIFAHDTKDDRKYGDFEWVGQNVAKFAGEIITRGAELWFIQVSGFTFENS 123
[Close]
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000017389.1 (SMESG000017389.1)

HSP 1 Score: 182.956 bits (463), Expect = 1.401e-59
Identity = 88/119 (73.95%), Postives = 98/119 (82.35%), Query Frame = 2 
Query:    2 IKVGFVLVVLEFCSGDLSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARGVELWFNQVSGFTFENS 358
            IK+ FVLVVL+FCSGDLS  +K+++L +HN YR  V     S  P ASNMQ MVW+E+SASKAQIYADKCIFAHDT DDRKYGDFEWVGQNVAKFAG+ I RG ELWF QVSGFTFENS
Sbjct:   35 IKIAFVLVVLDFCSGDLSEKEKNLLLNIHNEYRGDVAIGQVSGQPTASNMQLMVWNEKSASKAQIYADKCIFAHDTKDDRKYGDFEWVGQNVAKFAGEIITRGAELWFIQVSGFTFENS 153
[Close]
BLAST of Venom allergen 5 vs. Planmine SMEST
Match: SMESG000000477.1 (SMESG000000477.1)

HSP 1 Score: 174.866 bits (442), Expect = 1.158e-56
Identity = 85/119 (71.43%), Postives = 95/119 (79.83%), Query Frame = 2 
Query:    2 IKVGFVLVVLEFCSGDLSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNMQQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKIARGVELWFNQVSGFTFENS 358
            IKV FVLVVL+FCSGD+S ++   +L  HN YRQ V      + P ASNMQ+M W+E+SASKAQI ADKCIFAHDT DDRKYGDFEWVGQNVAKFA + IARG ELWFNQ SGFTFENS
Sbjct:    5 IKVAFVLVVLDFCSGDISLNEITQLLVSHNKYRQSVAKGEVPNQPKASNMQKMDWNEKSASKAQITADKCIFAHDTKDDRKYGDFEWVGQNVAKFAEENIARGAELWFNQASGFTFENS 123
The following BLAST results are available for this feature:
BLAST of Venom allergen 5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PI162.688e-829.59peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGN... [more]
PI162.688e-829.59peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGN... [more]
PI162.688e-829.59peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGN... [more]
CRISPLD21.607e-730.39cysteine rich secretory protein LCCL domain contai... [more]
CRISPLD21.631e-730.39cysteine rich secretory protein LCCL domain contai... [more]
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BLAST of Venom allergen 5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Venom allergen 5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG84836.909e-1233.04gene:FBgn0038126 transcript:FBtr0082782[more]
CG94001.459e-627.50gene:FBgn0030562 transcript:FBtr0073920[more]
CG326793.464e-632.73gene:FBgn0052679 transcript:FBtr0071469[more]
Ag5r4.578e-624.51gene:FBgn0015010 transcript:FBtr0333658[more]
Ag5r4.578e-624.51gene:FBgn0015010 transcript:FBtr0073937[more]
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BLAST of Venom allergen 5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
crispld28.505e-832.65cysteine-rich secretory protein LCCL domain contai... [more]
r3hdml7.299e-727.88R3H domain containing-like [Source:ZFIN;Acc:ZDB-GE... [more]
r3hdml7.299e-727.88R3H domain containing-like [Source:ZFIN;Acc:ZDB-GE... [more]
crispld1a7.922e-729.41cysteine-rich secretory protein LCCL domain contai... [more]
crispld1a8.040e-729.41cysteine-rich secretory protein LCCL domain contai... [more]
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BLAST of Venom allergen 5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
crispld28.821e-831.63cysteine-rich secretory protein LCCL domain contai... [more]
crispld28.859e-831.63cysteine-rich secretory protein LCCL domain contai... [more]
crispld28.867e-831.63cysteine-rich secretory protein LCCL domain contai... [more]
CRISPLD13.522e-730.39cysteine-rich secretory protein LCCL domain contai... [more]
CRISPLD13.606e-730.39cysteine-rich secretory protein LCCL domain contai... [more]
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BLAST of Venom allergen 5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Crispld21.115e-832.65cysteine-rich secretory protein LCCL domain contai... [more]
Crispld21.115e-832.65cysteine-rich secretory protein LCCL domain contai... [more]
Crispld21.115e-832.65cysteine-rich secretory protein LCCL domain contai... [more]
Pi163.503e-838.46peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:... [more]
Pi164.021e-838.46peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:... [more]
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BLAST of Venom allergen 5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|W4VS53|CRVP_TRILK3.827e-1541.51CRISP/Allergen/PR-1 OS=Trittame loki OX=1295018 PE... [more]
sp|P0DPV2|VA558_SCODE3.375e-1335.79Scoloptoxin SSD558 OS=Scolopendra dehaani OX=26097... [more]
sp|P0DPU1|VA576_SCODE3.670e-1335.79Scoloptoxin SSD976 OS=Scolopendra dehaani OX=26097... [more]
sp|P0DPU0|VA552_SCODE3.286e-1235.79Scoloptoxin SSD552 OS=Scolopendra dehaani OX=26097... [more]
sp|P85840|VA5_TITSE1.184e-1039.74Venom allergen 5 (Fragments) OS=Tityus serrulatus ... [more]
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BLAST of Venom allergen 5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
I1ZI858.877e-5774.78GLI pathogenesis related-1 (Fragment) OS=Schmidtea... [more]
A0A1C9ZZV51.031e-1536.52SCP domain-containing protein (Fragment) OS=Dugesi... [more]
A0A482XQ641.100e-1541.23SCP domain-containing protein OS=Laodelphax striat... [more]
A0A0R3UG821.882e-1536.36Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A4E0R0B32.659e-1538.66Pathogenesis protein PRMS OS=Fasciola hepatica OX=... [more]
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BLAST of Venom allergen 5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000032496.18.345e-1032.99pep primary_assembly:Astyanax_mexicanus-2.0:18:323... [more]
crispld22.996e-832.65cysteine-rich secretory protein LCCL domain contai... [more]
crispld1a5.557e-829.13cysteine-rich secretory protein LCCL domain contai... [more]
r3hdml1.411e-729.81R3H domain containing like [Source:NCBI gene;Acc:1... [more]
pi15a1.922e-729.13peptidase inhibitor 15-A-like [Source:NCBI gene;Ac... [more]
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BLAST of Venom allergen 5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Venom allergen 5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Venom allergen 5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Venom allergen 5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000020674.22.998e-828.44cysteine-rich venom protein pseudecin-like [Source... [more]
ENSORLT00000020676.24.504e-828.70cysteine-rich venom protein pseudecin-like [Source... [more]
crispld21.972e-731.63cysteine rich secretory protein LCCL domain contai... [more]
crispld1a5.639e-728.43cysteine rich secretory protein LCCL domain contai... [more]
crispld1a5.679e-728.43cysteine rich secretory protein LCCL domain contai... [more]
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BLAST of Venom allergen 5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000017386.11.617e-6176.47SMESG000017386.1[more]
SMESG000017383.14.785e-6172.27SMESG000017383.1[more]
SMESG000017389.12.828e-6073.95SMESG000017389.1[more]
SMESG000017389.11.401e-5973.95SMESG000017389.1[more]
SMESG000000477.11.158e-5671.43SMESG000000477.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013734 ID=SMED30013734|Name=Venom allergen 5|organism=Schmidtea mediterranea sexual|type=transcript|length=359bp
AATTAAAGTCGGATTTGTTTTAGTTGTTCTAGAATTTTGTTCTGGAGATC
TTTCTAATGACGATAAAGACATAATCTTGACTCTCCACAACATGTACCGA
CAATATGTGGTCTATGAAGTATTTTCAGATATACCCGTCGCATCGAACAT
GCAGCAAATGGTATGGAGTGAAGAATCCGCGTCAAAAGCGCAGATCTACG
CGGATAAATGCATATTTGCTCACGACACAATAGATGACCGTAAATACGGA
GATTTTGAATGGGTTGGACAGAATGTTGCTAAGTTCGCTGGACAAAAAAT
TGCCAGAGGCGTCGAATTGTGGTTTAATCAGGTTTCCGGATTCACATTTG
AAAATAGTG
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protein sequence of SMED30013734-orf-1

>SMED30013734-orf-1 ID=SMED30013734-orf-1|Name=SMED30013734-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=119bp
IKVGFVLVVLEFCSGDLSNDDKDIILTLHNMYRQYVVYEVFSDIPVASNM
QQMVWSEESASKAQIYADKCIFAHDTIDDRKYGDFEWVGQNVAKFAGQKI
ARGVELWFNQVSGFTFENS
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000421tail
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR035940CAP_sf
IPR034117SCP_CRISP
IPR014044CAP_domain
Vocabulary: cellular component
TermDefinition
GO:0005576extracellular region
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035940CAP superfamilyGENE3DG3DSA:3.40.33.10coord: 3..42
e-value: 3.9E-9
score: 38.2
IPR035940CAP superfamilySUPERFAMILYSSF55797PR-1-likecoord: 9..41
IPR034117Cysteine-rich secretory protein, SCP domainCDDcd05383CAP_CRISPcoord: 11..41
e-value: 5.4095E-8
score: 43.1111