Proteasome inhibitor PI31 subunit

Overview
NameProteasome inhibitor PI31 subunit
Smed IDSMED30013726
Length (bp)952
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Proteasome inhibitor PI31 subunit (SMED30013726) t-SNE clustered cells

Violin plots show distribution of expression levels for Proteasome inhibitor PI31 subunit (SMED30013726) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Proteasome inhibitor PI31 subunit (SMED30013726) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Proteasome inhibitor PI31 subunit (SMED30013726) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013726

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
body wall musculatureSMED30013726h1SMcG0008779 dd_Smed_v4_5145_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30013726h1SMcG0008779 dd_Smed_v4_5145_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Proteasome inhibitor PI31 subunit vs. Ensembl Mouse
Match: Psmf1 (proteasome (prosome, macropain) inhibitor subunit 1 [Source:MGI Symbol;Acc:MGI:1346072])

HSP 1 Score: 51.9878 bits (123), Expect = 3.254e-7
Identity = 56/211 (26.54%), Postives = 84/211 (39.81%), Query Frame = 2 
Query:   89 LEYLFETHKSQLNKPSDYLALLCH-SMLLDRKVKLKNG---------KAILPDEWNQTDPI-RLAYLCEDGT-ELNVSVVETAGDAFISVTESKSDHLGHLEVPMDDFINTSPNLLLDQSYPQLKELRKRFEENIWSIIFPVXXXXXXXXXXECVPSQVPSVCQPYQ---RRPNPLIDDRFSGPPSPFDYGRRDLDPFG------IGDPLR 658
            LE LF +    ++ P D L    H  ++ +    L  G           +LP +WN    +  L Y  +DG  +L +  V       I+V E  +  +  L + +DD+I+        ++Y   +ELR +    I + I    +K+     P   P        P Q    RP+      +  P SPF  G  DLDPFG      I DPLR
Sbjct:    4 LEVLFASAAPSMSCPQDALVCFLHWEVVTNGYYALGTGDQPGPSDKKSELLPAKWNSNKELYALRYESKDGARKLLLKAVSVENGMIINVLELGTQQVADLTLNLDDYIDAEDLSDFHRTYKNSEELRSQIRSGIITPIHEQWEKARANSPPREFPPATAREVDPLQISSHRPHTSRQPAWRDPLSPFAVGGDDLDPFGCQRGGMIVDPLR 214
[Close]
BLAST of Proteasome inhibitor PI31 subunit vs. Ensembl Mouse
Match: Psmf1 (proteasome (prosome, macropain) inhibitor subunit 1 [Source:MGI Symbol;Acc:MGI:1346072])

HSP 1 Score: 51.9878 bits (123), Expect = 3.254e-7
Identity = 56/211 (26.54%), Postives = 84/211 (39.81%), Query Frame = 2 
Query:   89 LEYLFETHKSQLNKPSDYLALLCH-SMLLDRKVKLKNG---------KAILPDEWNQTDPI-RLAYLCEDGT-ELNVSVVETAGDAFISVTESKSDHLGHLEVPMDDFINTSPNLLLDQSYPQLKELRKRFEENIWSIIFPVXXXXXXXXXXECVPSQVPSVCQPYQ---RRPNPLIDDRFSGPPSPFDYGRRDLDPFG------IGDPLR 658
            LE LF +    ++ P D L    H  ++ +    L  G           +LP +WN    +  L Y  +DG  +L +  V       I+V E  +  +  L + +DD+I+        ++Y   +ELR +    I + I    +K+     P   P        P Q    RP+      +  P SPF  G  DLDPFG      I DPLR
Sbjct:    4 LEVLFASAAPSMSCPQDALVCFLHWEVVTNGYYALGTGDQPGPSDKKSELLPAKWNSNKELYALRYESKDGARKLLLKAVSVENGMIINVLELGTQQVADLTLNLDDYIDAEDLSDFHRTYKNSEELRSQIRSGIITPIHEQWEKARANSPPREFPPATAREVDPLQISSHRPHTSRQPAWRDPLSPFAVGGDDLDPFGCQRGGMIVDPLR 214
[Close]
BLAST of Proteasome inhibitor PI31 subunit vs. UniProt/SwissProt
Match: sp|Q8BHL8|PSMF1_MOUSE (Proteasome inhibitor PI31 subunit OS=Mus musculus OX=10090 GN=Psmf1 PE=1 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 2.276e-6
Identity = 56/211 (26.54%), Postives = 84/211 (39.81%), Query Frame = 2 
Query:   89 LEYLFETHKSQLNKPSDYLALLCH-SMLLDRKVKLKNG---------KAILPDEWNQTDPI-RLAYLCEDGT-ELNVSVVETAGDAFISVTESKSDHLGHLEVPMDDFINTSPNLLLDQSYPQLKELRKRFEENIWSIIFPVXXXXXXXXXXECVPSQVPSVCQPYQ---RRPNPLIDDRFSGPPSPFDYGRRDLDPFG------IGDPLR 658
            LE LF +    ++ P D L    H  ++ +    L  G           +LP +WN    +  L Y  +DG  +L +  V       I+V E  +  +  L + +DD+I+        ++Y   +ELR +    I + I    +K+     P   P        P Q    RP+      +  P SPF  G  DLDPFG      I DPLR
Sbjct:    4 LEVLFASAAPSMSCPQDALVCFLHWEVVTNGYYALGTGDQPGPSDKKSELLPAKWNSNKELYALRYESKDGARKLLLKAVSVENGMIINVLELGTQQVADLTLNLDDYIDAEDLSDFHRTYKNSEELRSQIRSGIITPIHEQWEKARANSPPREFPPATAREVDPLQISSHRPHTSRQPAWRDPLSPFAVGGDDLDPFGCQRGGMIVDPLR 214
[Close]
BLAST of Proteasome inhibitor PI31 subunit vs. UniProt/SwissProt
Match: sp|Q5XIU5|PSMF1_RAT (Proteasome inhibitor PI31 subunit OS=Rattus norvegicus OX=10116 GN=Psmf1 PE=1 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 2.636e-6
Identity = 56/211 (26.54%), Postives = 81/211 (38.39%), Query Frame = 2 
Query:   89 LEYLFETHKSQLNKPSDYLALLCH-SMLLDRKVKLKNG---------KAILPDEWNQTDPI-RLAYLCEDGT-ELNVSVVETAGDAFISVTESKSDHLGHLEVPMDDFINTSPNLLLDQSYPQLKELRKRFEENIWSIIFPVXXXXXXXXXXECVPSQVPSVCQPYQ---RRPNPLIDDRFSGPPSPFDYGRRDLDPFG------IGDPLR 658
            LE LF +    +  P D L    H  ++ +    L  G           +LP EWN    +  L Y  +DG  +L +  V       I+V E  +  +  L + +DD+I+        ++Y   +ELR R    I + I    +K      P   P        P +     P+      +  P SPF  G  DLDPFG      I DPLR
Sbjct:    4 LEVLFASAAPAMTCPQDALVCFLHWEVVTNGYYGLGTGDQPDPNDKKSELLPAEWNSNKELYALRYESKDGARKLLLKAVSVENGMIINVLEHGTQQVADLTLNLDDYIDAEDLSDFHRTYKNSEELRSRIRSGIITPIHEQWEKVRLSSPPREFPPATAREVDPLRISSHHPHTSRQPTWRDPLSPFAVGGEDLDPFGCQRGGMIVDPLR 214
[Close]
BLAST of Proteasome inhibitor PI31 subunit vs. UniProt/SwissProt
Match: sp|Q3SX30|PSMF1_BOVIN (Proteasome inhibitor PI31 subunit OS=Bos taurus OX=9913 GN=PSMF1 PE=2 SV=2)

HSP 1 Score: 50.0618 bits (118), Expect = 9.553e-6
Identity = 56/210 (26.67%), Postives = 84/210 (40.00%), Query Frame = 2 
Query:   89 LEYLFETHKSQLNKPSDYLALLCH-SMLLDRKVKLKNG---------KAILPDEWNQT-DPIRLAYLCEDGT-ELNVSVVETAGDAFISVTESKSDHLGHLEVPMDDFINTSPNLLLDQSYPQLKELRKRFEENIWSIIFPVXXXXXXXXXXECVPSQVPSV--CQPYQRRPNPLIDDRFSGPPSPFDYGRRDLDPFG------IGDPLR 658
            LE LF +    +    D L    H  ++      L  G           +LP EWN   D   L Y  +DG+ +L V  V       I+V E  S  +  L + ++D+I++   +   + Y   +ELR R    I + I    +K+      E  P+    V   + + + P+      +  P  PF  G  DLDPFG      I DPLR
Sbjct:    4 LEVLFASAAPAITCAQDALVCFLHWEVVTHGYYGLGAGDQPGPNDKKSELLPVEWNSNKDLYVLRYESKDGSRKLLVKAVTVENSMIINVLEHGSQQVSDLTLNLNDYIDSEHLVDFHRVYKNSEELRSRIVSGIITPIHEQWEKANLSPHREFPPATAREVDPLRIHPQHPHTSRQPTWCDPLGPFAVGGEDLDPFGCRRGGMIVDPLR 213
[Close]
BLAST of Proteasome inhibitor PI31 subunit vs. TrEMBL
Match: A0A564ZD47 (PI31_Prot_C domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS14459 PE=4 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 6.214e-19
Identity = 109/294 (37.07%), Postives = 141/294 (47.96%), Query Frame = 2 
Query:   83 SFLEYLFETHKSQLN-KPSDYLALLCHSMLLDRKVKLKNGK-----AILPDEWNQTDPIRLAY----LCEDGTE---LNVSVVETAGDAFISVTESKSDHLGHLEVPMDDFIN---TSPNLLLDQS--YPQLKELRKRFEENIWSIIFPVXXXXXXXXXXECVPSQVPSVCQPYQRRPNPLIDDRFSGPPSPFDYGRRDLDPFGIGDPLRHXXXXXXXXXMLFDP----RHRPSGSDNRPLYXXXXXXXXXXXXRIDPIYP--MXXXXXXXXXXXXXXXXXXXXXXXXWEDMYL 892
            S LEY+   H +Q N K S YLALLCH+ LL R  +L +       ++LP+ W + DPIR++Y    +  DG++   L +++ +T  + F++ T S +D  GHLE+     +      PN  +  S  + QL+      EEN+W  I P              PS   S C P  R   P  D  F   P+  DYGR DLDP G  D        GG GGM+ DP    R RP G  +     G   G  PPGAR DPI P  MP  G G        PDPD   PPGWEDMY+
Sbjct:   15 SVLEYVINLHSNQTNGKRSAYLALLCHADLLSRGARLADNDQPGEVSLLPNNWAELDPIRISYKSPKVNGDGSDEHILQLTISQTDDNVFLNATNSTTDKFGHLELIGPAHVRLDVVKPNSFIKPSDLFLQLESTLYEIEENLWDAILPKPSGFN--------PSSRQSSCNPPPRG-GPRSDSFFPPDPNLRDYGRSDLDPLGGND---WTSPLGGTGGMVIDPTQALRRRPFGRISDRFPRGGLGGTVPPGARFDPIGPPIMPPRGIGPRGSQFNNPDPDSAIPPGWEDMYM 296
[Close]
BLAST of Proteasome inhibitor PI31 subunit vs. TrEMBL
Match: G4VGG1 (Putative proteasome inhibitor OS=Schistosoma mansoni OX=6183 GN=Smp_066340.1 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.044e-16
Identity = 81/254 (31.89%), Postives = 113/254 (44.49%), Query Frame = 2 
Query:   77 DSSFLEYLFETHKSQLNKPSDYLALLCHSMLLDRKVKLKNGKA---ILPDEWNQTDPIRLAYL----------CEDGTELNVSVVETAGDAFISVTESKSDHLGHLEVPMDDFI--NTSPNLLLDQS---YPQLKELRKRFEENIWSIIFPVXXXXXXXXXXECVPSQVPSVCQPYQR-----RPN---------PLIDDRFS-----GPPSPFDYGRRDLDPFG-IGDPLRHXXXXXXXXXMLFDPRHRPSGS 724
            ++S L+Y+   H S     + ++ALL HS LL R  +     A   +L       DPIR++Y               ++L VSV ET    F++VT++ +D  GHLE+   + +  ++  NL    +   +PQL EL   FEEN+W  + P                  P+ C+P +      RPN         P +DD        GPP P DYGR DLDP   IG P R       GGGM+ DPR    GS
Sbjct:   26 ETSILDYVLSFHGSNCKNRASFIALLVHSELLSRGARPTEATARTAVLSQSCLFEDPIRMSYTLISRMKSDTSSTPCSQLQVSVAETDAHIFVNVTDTTTDKFGHLELSGIEHVCLDSVNNLTYGDANTVFPQLNELLDSFEENLWKPVLP-----------------SPAFCRPTETDRNEPRPNDDFHNKPRRPFVDDFIRAGATVGPPPP-DYGRSDLDPLASIGGPSR-------GGGMILDPRRIIPGS 254
[Close]
BLAST of Proteasome inhibitor PI31 subunit vs. TrEMBL
Match: G4VGG0 (Putative proteasome inhibitor OS=Schistosoma mansoni OX=6183 GN=Smp_066340.2 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.137e-16
Identity = 81/254 (31.89%), Postives = 113/254 (44.49%), Query Frame = 2 
Query:   77 DSSFLEYLFETHKSQLNKPSDYLALLCHSMLLDRKVKLKNGKA---ILPDEWNQTDPIRLAYL----------CEDGTELNVSVVETAGDAFISVTESKSDHLGHLEVPMDDFI--NTSPNLLLDQS---YPQLKELRKRFEENIWSIIFPVXXXXXXXXXXECVPSQVPSVCQPYQR-----RPN---------PLIDDRFS-----GPPSPFDYGRRDLDPFG-IGDPLRHXXXXXXXXXMLFDPRHRPSGS 724
            ++S L+Y+   H S     + ++ALL HS LL R  +     A   +L       DPIR++Y               ++L VSV ET    F++VT++ +D  GHLE+   + +  ++  NL    +   +PQL EL   FEEN+W  + P                  P+ C+P +      RPN         P +DD        GPP P DYGR DLDP   IG P R       GGGM+ DPR    GS
Sbjct:   26 ETSILDYVLSFHGSNCKNRASFIALLVHSELLSRGARPTEATARTAVLSQSCLFEDPIRMSYTLISRMKSDTSSTPCSQLQVSVAETDAHIFVNVTDTTTDKFGHLELSGIEHVCLDSVNNLTYGDANTVFPQLNELLDSFEENLWKPVLP-----------------SPAFCRPTETDRNEPRPNDDFHNKPRRPFVDDFIRAGATVGPPPP-DYGRSDLDPLASIGGPSR-------GGGMILDPRRIIPGS 254
[Close]
BLAST of Proteasome inhibitor PI31 subunit vs. TrEMBL
Match: A0A183LPN6 (PI31_Prot_C domain-containing protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS5761 PE=4 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.302e-16
Identity = 80/255 (31.37%), Postives = 110/255 (43.14%), Query Frame = 2 
Query:   77 DSSFLEYLFETHKSQLNKPSDYLALLCHSMLLDRKVK---LKNGKAILPDEWNQTDPIRLAY----------LCEDGTELNVSVVETAGDAFISVTESKSDHLGHLEV---------PMDDFINTSPNLLLDQSYPQLKELRKRFEENIWSIIFPVXXXXXXXXXXECVPSQVPSVCQPYQRRPNPLIDDRFSGPPSPF----------------DYGRRDLDPFG-IGDPLRHXXXXXXXXXMLFDPRHRPSGS 724
            ++S L+Y+   H S     + ++ALL HS LL R  +     +  A+L       DPIR++Y          L    ++L VSV ET    F++VT++ +D  GHLE+          +D+F     N +    +PQL EL   FEEN+W  + P            C  S+        +  P P  DD  + P  PF                DYGR DLDP   IG P R       GGGM+ DPRH   GS
Sbjct:   26 ETSILDYVLSFHGSNCKNRASFIALLVHSELLSRGARPAEATSRTAVLSSSCLAEDPIRISYALISRTKSDTLSTPCSQLQVSVAETDAHIFVNVTDTTTDKFGHLELSGIEHVCLDSVDNFTYGDANTV----FPQLNELLDSFEENLWKPVLP--------PPLSCRQSET------DKNEPRPSNDDLHNQPQIPFRGNFTRTEPSTGTLPPDYGRSDLDPLASIGGPSR-------GGGMILDPRHIIPGS 255
[Close]
BLAST of Proteasome inhibitor PI31 subunit vs. TrEMBL
Match: A0A0R3WL21 (PI31_Prot_C domain-containing protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS1442 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 3.991e-15
Identity = 70/219 (31.96%), Postives = 105/219 (47.95%), Query Frame = 2 
Query:   77 DSSFLEYLFETHKSQLN-KPSDYLALLCHSMLLDRKVKLKN-----GKAILPDEWNQTDPIRLAYLCEDGTELN--------------VSVVETAGDAFISVTESKSDHLGHLEVPMDDFIN---TSPNLLLD--QSYPQLKELRKRFEENIWSIIFPVXXXXXXXXXXECVPSQVPSVCQPYQRRPNPLIDDRFSGPPSPFDYGRRDLDPFGIGDPLR 658
            +SS L+Y+   H +Q N + S ++A+L H+ L+ R  KL         +ILP EW   DPIR+ Y   +  +LN              +SV ET    F+++T + ++  GHLE+     +     SP  +L   + Y QL+      +EN+W ++ P   KS  R +P C+ SQ P+   P   R    +D      P+  DYGR DLDP G  D  R
Sbjct:   14 ESSILDYVVNLHGAQANGRRSAFIAILSHACLIARGAKLPGTGHLQSTSILPSEWASNDPIRINYQLGNVEDLNLGNSVRDSSDQMLQLSVTETDDHIFVNLTNTTNEKFGHLELSGPAHVRLEFASPGSILQPIEIYSQLEVTLFEMDENLWKVVLPKPAKSSSRGTPFCL-SQ-PNTLHPLPTRSLSEVD------PTLRDYGRSDLDPLGAEDWTR 224
[Close]
BLAST of Proteasome inhibitor PI31 subunit vs. Planmine SMEST
Match: SMESG000076420.1 (SMESG000076420.1)

HSP 1 Score: 427.557 bits (1098), Expect = 4.871e-152
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 2 
Query:   71 MSDSSFLEYLFETHKSQLNKPSDYLALLCHSMLLDRKVKLKNGKAILPDEWNQTDPIRLAYLCEDGTELNVSVVETAGDAFISVTESKSDHLGHLEVPMDDFINTSPNLLLDQSYPQLKELRKRFEENIWSIIFPVXXXXXXXXXXECVPSQVPSVCQPYQRRPNPLIDDRFSGPPSPFDYGRRDLDPFGIGDPLRHXXXXXXXXXMLFDPRHRPSGSDNRPLYXXXXXXXXXXXXRIDPIYPMXXXXXXXXXXXXXXXXXXXXXXXXWEDMYL 892
            MSDSSFLEYLFETHKSQLNKPSDYLALLCHSMLLDRKVKLKNGKAILPDEWNQTDPIRLAYLCEDGTELNVSVVETAGDAFISVTESKSDHLGHLEVPMDDFINTSPNLLLDQSYPQLKELRKRFEENIWSIIFPVKKKSPPRRSPECVPSQVPSVCQPYQRRPNPLIDDRFSGPPSPFDYGRRDLDPFGIGDPLRHGGFGGGGGGMLFDPRHRPSGSDNRPLYPGAPPGAAPPGARIDPIYPMPQPGRGAPHRPAAPPDPDMPTPPGWEDMYL
Sbjct:    1 MSDSSFLEYLFETHKSQLNKPSDYLALLCHSMLLDRKVKLKNGKAILPDEWNQTDPIRLAYLCEDGTELNVSVVETAGDAFISVTESKSDHLGHLEVPMDDFINTSPNLLLDQSYPQLKELRKRFEENIWSIIFPVKKKSPPRRSPECVPSQVPSVCQPYQRRPNPLIDDRFSGPPSPFDYGRRDLDPFGIGDPLRHGGFGGGGGGMLFDPRHRPSGSDNRPLYPGAPPGAAPPGARIDPIYPMPQPGRGAPHRPAAPPDPDMPTPPGWEDMYL 274
The following BLAST results are available for this feature:
BLAST of Proteasome inhibitor PI31 subunit vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Proteasome inhibitor PI31 subunit vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Proteasome inhibitor PI31 subunit vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Proteasome inhibitor PI31 subunit vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Proteasome inhibitor PI31 subunit vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Proteasome inhibitor PI31 subunit vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Psmf13.254e-726.54proteasome (prosome, macropain) inhibitor subunit ... [more]
Psmf13.254e-726.54proteasome (prosome, macropain) inhibitor subunit ... [more]
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BLAST of Proteasome inhibitor PI31 subunit vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q8BHL8|PSMF1_MOUSE2.276e-626.54Proteasome inhibitor PI31 subunit OS=Mus musculus ... [more]
sp|Q5XIU5|PSMF1_RAT2.636e-626.54Proteasome inhibitor PI31 subunit OS=Rattus norveg... [more]
sp|Q3SX30|PSMF1_BOVIN9.553e-626.67Proteasome inhibitor PI31 subunit OS=Bos taurus OX... [more]
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BLAST of Proteasome inhibitor PI31 subunit vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A564ZD476.214e-1937.07PI31_Prot_C domain-containing protein OS=Hymenolep... [more]
G4VGG11.044e-1631.89Putative proteasome inhibitor OS=Schistosoma manso... [more]
G4VGG01.137e-1631.89Putative proteasome inhibitor OS=Schistosoma manso... [more]
A0A183LPN61.302e-1631.37PI31_Prot_C domain-containing protein OS=Schistoso... [more]
A0A0R3WL213.991e-1531.96PI31_Prot_C domain-containing protein OS=Hydatigen... [more]
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BLAST of Proteasome inhibitor PI31 subunit vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Proteasome inhibitor PI31 subunit vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Proteasome inhibitor PI31 subunit vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Proteasome inhibitor PI31 subunit vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Proteasome inhibitor PI31 subunit vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Proteasome inhibitor PI31 subunit vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000076420.14.871e-152100.00SMESG000076420.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013726 ID=SMED30013726|Name=Proteasome inhibitor PI31 subunit|organism=Schmidtea mediterranea sexual|type=transcript|length=952bp
GAATAATTTAATTTTGATTAATTGGTTCAAATTTTGCGAAATATTATTTA
GATATTTAATCTAAGTGATTATGTCAGATTCAAGTTTTCTTGAATATCTG
TTTGAGACTCACAAGTCACAATTGAATAAACCTTCAGATTATTTGGCACT
TTTATGTCATTCTATGTTGCTTGATAGAAAAGTTAAATTAAAAAATGGTA
AAGCAATTTTACCGGATGAATGGAATCAAACTGATCCAATTCGACTTGCT
TATTTGTGTGAAGATGGAACTGAATTAAATGTTTCTGTTGTTGAGACTGC
TGGAGATGCTTTCATTTCGGTCACTGAGTCCAAATCTGACCATCTGGGGC
ATCTGGAGGTGCCGATGGATGATTTTATTAATACATCTCCCAATCTCTTA
CTGGATCAGTCTTATCCTCAGTTGAAAGAACTTCGAAAACGTTTCGAGGA
AAATATTTGGTCAATAATATTCCCGGTGAAGAAAAAGAGTCCCCCAAGGC
GCTCGCCCGAATGCGTTCCCTCTCAGGTTCCGTCAGTGTGCCAGCCATAT
CAACGACGGCCGAATCCATTGATTGATGATCGATTTAGCGGGCCTCCCAG
TCCGTTCGATTACGGCCGCAGAGATCTCGATCCGTTCGGTATCGGTGATC
CCCTGAGGCACGGCGGTTTCGGTGGCGGTGGTGGCGGAATGCTGTTTGAT
CCCCGGCACCGCCCGTCCGGTAGCGACAACCGGCCGCTATATCCCGGTGC
CCCTCCTGGCGCCGCTCCTCCTGGGGCAAGGATCGATCCCATATATCCGA
TGCCGCAACCCGGACGAGGGGCTCCCCACCGCCCTGCCGCTCCCCCTGAT
CCCGATATGCCGACACCGCCAGGATGGGAGGACATGTACTTGTGAATTTA
GTTACCAAATGTTTCATTATCATCAATAAATATTGCTTGTTGAGATCGGA
AG
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protein sequence of SMED30013726-orf-1

>SMED30013726-orf-1 ID=SMED30013726-orf-1|Name=SMED30013726-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=275bp
MSDSSFLEYLFETHKSQLNKPSDYLALLCHSMLLDRKVKLKNGKAILPDE
WNQTDPIRLAYLCEDGTELNVSVVETAGDAFISVTESKSDHLGHLEVPMD
DFINTSPNLLLDQSYPQLKELRKRFEENIWSIIFPVKKKSPPRRSPECVP
SQVPSVCQPYQRRPNPLIDDRFSGPPSPFDYGRRDLDPFGIGDPLRHGGF
GGGGGGMLFDPRHRPSGSDNRPLYPGAPPGAAPPGARIDPIYPMPQPGRG
APHRPAAPPDPDMPTPPGWEDMYL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000240body wall musculature
Vocabulary: INTERPRO
TermDefinition
IPR021625PI31_Prot_N
Vocabulary: cellular component
TermDefinition
GO:0000502proteasome complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.1000.30coord: 2..145
e-value: 2.5E-13
score: 51.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 216..274
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 226..266
NoneNo IPR availablePANTHERPTHR13266PROTEASOME INHIBITORcoord: 1..273
IPR021625PI31 proteasome regulator, N-terminalPFAMPF11566PI31_Prot_Ncoord: 15..135
e-value: 2.6E-9
score: 37.1