Mediator of RNA polymerase II transcription subunit 8

Overview
NameMediator of RNA polymerase II transcription subunit 8
Smed IDSMED30013672
Length (bp)708
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Mediator of RNA polymerase II transcription subunit 8 (SMED30013672) t-SNE clustered cells

Violin plots show distribution of expression levels for Mediator of RNA polymerase II transcription subunit 8 (SMED30013672) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Mediator of RNA polymerase II transcription subunit 8 (SMED30013672) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Mediator of RNA polymerase II transcription subunit 8 (SMED30013672) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013672

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30013672SMESG000080873.1 SmedASXL_011363SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30013672SMESG000080873.1 dd_Smed_v4_5511_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30013672SMESG000080873.1 SMED_05676_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30013672SMESG000080873.1 SMED_05676_V2_1GPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Human
Match: MED8 (mediator complex subunit 8 [Source:HGNC Symbol;Acc:HGNC:19971])

HSP 1 Score: 75.485 bits (184), Expect = 1.211e-15
Identity = 45/150 (30.00%), Postives = 81/150 (54.00%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNAS-NKVVDSALKELSSLKNQIDVDNTEKAIKQNTMTEIQELFNCMSNGKGL 564
            W   L+ F LL+ +LN + K +  +K     N V+IP+ L+P+ D++L+  T+ R+  F++EV P +LRT  DP+V+   K+    A+ + A+   KQ  S NK+  + L+++S  + + +        +    T+   L   ++ GKGL
Sbjct:   42 WPSVLDSFALLSGQLNTLNKVLKHEKTPLFRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQIQSLNKMCSNLLEKISKEERESESGGLRPNKQTFNPTDTNALVAAVAFGKGL 191
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Human
Match: MED8 (mediator complex subunit 8 [Source:HGNC Symbol;Acc:HGNC:19971])

HSP 1 Score: 75.485 bits (184), Expect = 1.630e-15
Identity = 45/150 (30.00%), Postives = 81/150 (54.00%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNAS-NKVVDSALKELSSLKNQIDVDNTEKAIKQNTMTEIQELFNCMSNGKGL 564
            W   L+ F LL+ +LN + K +  +K     N V+IP+ L+P+ D++L+  T+ R+  F++EV P +LRT  DP+V+   K+    A+ + A+   KQ  S NK+  + L+++S  + + +        +    T+   L   ++ GKGL
Sbjct:   42 WPSVLDSFALLSGQLNTLNKVLKHEKTPLFRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQIQSLNKMCSNLLEKISKEERESESGGLRPNKQTFNPTDTNALVAAVAFGKGL 191
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Fly
Match: MED8 (gene:FBgn0034503 transcript:FBtr0086297)

HSP 1 Score: 77.7962 bits (190), Expect = 6.389e-17
Identity = 45/155 (29.03%), Postives = 85/155 (54.84%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQ-NASNKVVDSALKELSSLKNQIDVDNTEKAIKQNT--MTEIQELFNCMSNGKGLKLS 573
            W  +L+ F ++++ L  + K +  ++   L N  ++P+ ++ + D  LIN+T+ R+  F++++ P YLRT  DP  +    +   KA+NL+ +   KQ    NKVV   L  +S  + + +++++ +   Q T  M + Q L   +  GKGLKL+
Sbjct:   42 WPTFLDNFAIISSHLTGLTKILAKEQCPPLRNRTVLPLLVSMDRDDTLINITEGRVPVFSHDIVPDYLRTRPDPITEQKMLQNEQKAANLTNDAAMKQVTQYNKVVSHVLDMVSKAREEWEIESSSRTGIQQTSSMADTQLLVAAVGMGKGLKLT 196
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Zebrafish
Match: med8 (mediator complex subunit 8 [Source:NCBI gene;Acc:404615])

HSP 1 Score: 77.7962 bits (190), Expect = 1.090e-16
Identity = 48/154 (31.17%), Postives = 85/154 (55.19%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQ-NASNKVVDSALKELSSLKNQIDVDNTEKAIKQNTMT----EIQELFNCMSNGKGL 564
            W   L+ F L++ +LN I K +  +K     + V+IP+ L+P+ D+ L  LT+ R+  F++E+ P +LRT  DP+V+   K    +A+ +  E+  KQ  A NK+  + L++L++ +   D D+   A++QN  +    +   L   +  GKGL
Sbjct:   43 WPSVLDNFALISGQLNTINKLLRNEKTPSYKSQVIIPLLLSPDRDEELAKLTEHRVPVFSHEIVPDHLRTKPDPEVEEQEKHLSAEAARIGPEVAQKQIQALNKLCSNLLEKLNNPRE--DRDSETSALRQNKPSFNPADTNALVAAVGFGKGL 194
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Xenopus
Match: elob (elongin B [Source:Xenbase;Acc:XB-GENE-959169])

HSP 1 Score: 77.411 bits (189), Expect = 2.060e-16
Identity = 46/150 (30.67%), Postives = 85/150 (56.67%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNAS-NKVVDSALKELSSLKNQIDVDNTEKAIKQNTMTEIQELFNCMSNGKGL 564
            W   L+ F LL+ +LN + K +  +K   L N V+IP+ L+P+ D+ ++ LT+ R+  F++EV P +LRT  DP V+   K+   +A+ +++E   KQ  S NK+  + L ++S  + + ++ +  +  +    T+   L   ++ GKGL
Sbjct:   42 WPSVLDSFALLSGQLNTLNKVLKNEKTPLLRNQVIIPLLLSPDRDEEIMRLTEGRVPVFSHEVVPDHLRTKPDPDVEELEKQLSAEAARITSEAAQKQVQSMNKMCSNLLDKISKEERESELGSLRQNKQTFNPTDTNALVAAVAFGKGL 191
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Xenopus
Match: elob (elongin B [Source:Xenbase;Acc:XB-GENE-959169])

HSP 1 Score: 77.411 bits (189), Expect = 2.060e-16
Identity = 46/150 (30.67%), Postives = 85/150 (56.67%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNAS-NKVVDSALKELSSLKNQIDVDNTEKAIKQNTMTEIQELFNCMSNGKGL 564
            W   L+ F LL+ +LN + K +  +K   L N V+IP+ L+P+ D+ ++ LT+ R+  F++EV P +LRT  DP V+   K+   +A+ +++E   KQ  S NK+  + L ++S  + + ++ +  +  +    T+   L   ++ GKGL
Sbjct:   42 WPSVLDSFALLSGQLNTLNKVLKNEKTPLLRNQVIIPLLLSPDRDEEIMRLTEGRVPVFSHEVVPDHLRTKPDPDVEELEKQLSAEAARITSEAAQKQVQSMNKMCSNLLDKISKEERESELGSLRQNKQTFNPTDTNALVAAVAFGKGL 191
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Mouse
Match: Med8 (mediator complex subunit 8 [Source:MGI Symbol;Acc:MGI:1915269])

HSP 1 Score: 73.559 bits (179), Expect = 4.214e-15
Identity = 44/150 (29.33%), Postives = 80/150 (53.33%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNAS-NKVVDSALKELSSLKNQIDVDNTEKAIKQNTMTEIQELFNCMSNGKGL 564
            W   L+ F LL+ +LN + K +  +K     N V+IP+ L+P+ D++L+  T+ R+  F++EV P +LRT  DP+V+   K+    A+ + A+   KQ  S NK+  + L+++S  + + +        +     +   L   ++ GKGL
Sbjct:   42 WPSVLDSFALLSGQLNTLNKVLKHEKTPLFRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQIQSLNKMCSNLLEKISKEERESESGGLRPNKQTFNPGDTNALVAAVAFGKGL 191
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Mouse
Match: Med8 (mediator complex subunit 8 [Source:MGI Symbol;Acc:MGI:1915269])

HSP 1 Score: 69.3218 bits (168), Expect = 9.648e-14
Identity = 38/110 (34.55%), Postives = 67/110 (60.91%), Query Frame = 1 
Query:  130 LNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNAS-NKVVDSALKELS 456
            L+ F LL+ +LN + K +  +K     N V+IP+ L+P+ D++L+  T+ R+  F++EV P +LRT  DP+V+   K+    A+ + A+   KQ  S NK+  + L+++S
Sbjct:   62 LDSFALLSGQLNTLNKVLKHEKTPLFRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQIQSLNKMCSNLLEKIS 171
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. UniProt/SwissProt
Match: sp|Q7Q6D6|MED8_ANOGA (Mediator of RNA polymerase II transcription subunit 8 OS=Anopheles gambiae OX=7165 GN=MED8 PE=3 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 6.258e-17
Identity = 48/152 (31.58%), Postives = 82/152 (53.95%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNAS-NKVVDSALKELSSLKNQIDVD-NTEKAIKQ-NTMTEIQELFNCMSNGKGL 564
            W  +L+ F L+++ L  ++K + T+    L NL ++P+ L P  D+ L+ LT+ R+  F++++ P YLRT  DP  +  +     KA+NL+ E   KQ A  NKV+      +S  K   + + +T   I+Q ++M + Q L   +  G GL
Sbjct:   42 WPTFLDNFALISSHLTGLMKILSTEIGTPLRNLTVLPLMLTPERDEALLQLTEGRVPIFSHDLAPDYLRTKPDPGAESRQAAHEAKANNLTVEASMKQVAQYNKVISHVWDIISKAKEDWENESSTRPGIQQTSSMADTQALVAAVGLGNGL 193
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. UniProt/SwissProt
Match: sp|A1ZBT5|MED8_DROME (Mediator of RNA polymerase II transcription subunit 8 OS=Drosophila melanogaster OX=7227 GN=MED8 PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 6.405e-16
Identity = 45/155 (29.03%), Postives = 85/155 (54.84%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQ-NASNKVVDSALKELSSLKNQIDVDNTEKAIKQNT--MTEIQELFNCMSNGKGLKLS 573
            W  +L+ F ++++ L  + K +  ++   L N  ++P+ ++ + D  LIN+T+ R+  F++++ P YLRT  DP  +    +   KA+NL+ +   KQ    NKVV   L  +S  + + +++++ +   Q T  M + Q L   +  GKGLKL+
Sbjct:   42 WPTFLDNFAIISSHLTGLTKILAKEQCPPLRNRTVLPLLVSMDRDDTLINITEGRVPVFSHDIVPDYLRTRPDPITEQKMLQNEQKAANLTNDAAMKQVTQYNKVVSHVLDMVSKAREEWEIESSSRTGIQQTSSMADTQLLVAAVGMGKGLKLT 196
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. UniProt/SwissProt
Match: sp|Q6NYT1|MED8_DANRE (Mediator of RNA polymerase II transcription subunit 8 OS=Danio rerio OX=7955 GN=med8 PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 7.822e-16
Identity = 48/154 (31.17%), Postives = 85/154 (55.19%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQ-NASNKVVDSALKELSSLKNQIDVDNTEKAIKQNTMT----EIQELFNCMSNGKGL 564
            W   L+ F L++ +LN I K +  +K     + V+IP+ L+P+ D+ L  LT+ R+  F++E+ P +LRT  DP+V+   K    +A+ +  E+  KQ  A NK+  + L++L++ +   D D+   A++QN  +    +   L   +  GKGL
Sbjct:   43 WPSVLDNFALISGQLNTINKLLRNEKTPSYKSQVIIPLLLSPDRDEELAKLTEHRVPVFSHEIVPDHLRTKPDPEVEEQEKHLSAEAARIGPEVAQKQIQALNKLCSNLLEKLNNPRE--DRDSETSALRQNKPSFNPADTNALVAAVGFGKGL 194
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. UniProt/SwissProt
Match: sp|Q6DD30|MED8B_XENLA (Mediator of RNA polymerase II transcription subunit 8-B OS=Xenopus laevis OX=8355 GN=med8-b PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 8.974e-16
Identity = 47/150 (31.33%), Postives = 84/150 (56.00%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNAS-NKVVDSALKELSSLKNQIDVDNTEKAIKQNTMTEIQELFNCMSNGKGL 564
            W   L+ F LL+ +LN + K +  +K   L N V+IP+ L+P+ D+ ++ LT+ R+  F++EV P +LRT  DP V+   K+    A+ L++E   KQ  S NK+  + L ++S  + + ++ +  +  +    T+   L   ++ GKGL
Sbjct:   42 WPSVLDSFALLSGQLNTLNKVLKNEKTPLLRNQVIIPLLLSPDRDEEIMRLTEGRVPVFSHEVVPDHLRTKPDPDVEELEKQISADATRLTSEAAQKQVQSMNKMCSNLLDKISKEERESELGSLRQNKQTFNPTDTNALVAAVAFGKGL 191
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. UniProt/SwissProt
Match: sp|Q08BU1|MED8_XENTR (Mediator of RNA polymerase II transcription subunit 8 OS=Xenopus tropicalis OX=8364 GN=med8 PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.091e-15
Identity = 46/150 (30.67%), Postives = 85/150 (56.67%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNAS-NKVVDSALKELSSLKNQIDVDNTEKAIKQNTMTEIQELFNCMSNGKGL 564
            W   L+ F LL+ +LN + K +  +K   L N V+IP+ L+P+ D+ ++ LT+ R+  F++EV P +LRT  DP V+   K+   +A+ +++E   KQ  S NK+  + L ++S  + + ++ +  +  +    T+   L   ++ GKGL
Sbjct:   42 WPSVLDSFALLSGQLNTLNKVLKNEKTPLLRNQVIIPLLLSPDRDEEIMRLTEGRVPVFSHEVVPDHLRTKPDPDVEELEKQLSAEAARITSEAAQKQVQSMNKMCSNLLDKISKEERESELGSLRQNKQTFNPTDTNALVAAVAFGKGL 191
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. TrEMBL
Match: A0A183A961 (Mediator of RNA polymerase II transcription subunit 8 OS=Echinostoma caproni OX=27848 GN=MED8 PE=3 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.486e-23
Identity = 58/157 (36.94%), Postives = 96/157 (61.15%), Query Frame = 1 
Query:  109 NHEWSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSK-QNASNKVVDSALKELSSLKNQIDVDNTEKAIKQNTMTEIQELFNCM----SNGKGL 564
            N +W +YL+   L A+EL+ I K + +D+ +    LV+ P  LNP PD  L   T++R+  F ++  PQYLRT LDPK++   + QL +AS++ +E ++K  N +N+ +DS+LKE++ LK +++ D ++   +QN  T   +  N M    + GKGL
Sbjct:   39 NCDWPRYLSTLALCASELSEIRKVLESDRFVSEHTLVLTPTLLNPEPDPTLAKATEERLSLFNHDTVPQYLRTKLDPKLETQCQAQLNRASSMPSEQLTKLVNLANRAIDSSLKEVNLLKQELEADFSD---RQNKSTSSADDLNAMVAAITLGKGL 192
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. TrEMBL
Match: A0A0X3P6B3 (Mediator of RNA polymerase II transcription subunit 8 OS=Schistocephalus solidus OX=70667 GN=MED8 PE=3 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.975e-23
Identity = 56/138 (40.58%), Postives = 85/138 (61.59%), Query Frame = 1 
Query:  100 RISNHEWSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSK-QNASNKVVDSALKELSSLKNQIDVDNTEKAIKQN 510
            +  + +W KYLN  GL A+EL  I K + T++     +LV+ PV LNP PD  L   T +R+  F ++  PQYLRT L P+V+  R  Q  KAS +  E  +K  N SN+++DS LKE+++LK ++++D +EK  K +
Sbjct:   36 QCDSCDWPKYLNTLGLCASELVEIRKVLETERFSLAQSLVLTPVRLNPEPDPALAKATDERLPRFDHDTAPQYLRTKLAPQVESQRMAQNAKASAILPEQATKLVNLSNRLLDSGLKEVNALKQELEMDLSEKTFKSS 173
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. TrEMBL
Match: A0A3P7CF65 (Mediator of RNA polymerase II transcription subunit 8 OS=Schistocephalus solidus OX=70667 GN=MED8 PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.171e-23
Identity = 56/135 (41.48%), Postives = 84/135 (62.22%), Query Frame = 1 
Query:  109 NHEWSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSK-QNASNKVVDSALKELSSLKNQIDVDNTEKAIKQN 510
            + +W KYLN  GL A+EL  I K + T++     +LV+ PV LNP PD  L   T +R+  F ++  PQYLRT L P+V+  R  Q  KAS +  E  +K  N SN+++DS LKE+++LK ++++D +EK  K +
Sbjct:   18 SCDWPKYLNTLGLCASELVEIRKVLETERFSLAQSLVLTPVRLNPEPDPALAKATDERLPRFDHDTAPQYLRTKLAPQVESQRMAQNSKASAILPEQATKLVNLSNRLLDSGLKEVNALKQELEMDLSEKTFKSS 152
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. TrEMBL
Match: A0A183SJ50 (Mediator of RNA polymerase II transcription subunit 8 OS=Schistocephalus solidus OX=70667 GN=MED8 PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.441e-23
Identity = 56/135 (41.48%), Postives = 84/135 (62.22%), Query Frame = 1 
Query:  109 NHEWSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSK-QNASNKVVDSALKELSSLKNQIDVDNTEKAIKQN 510
            + +W KYLN  GL A+EL  I K + T++     +LV+ PV LNP PD  L   T +R+  F ++  PQYLRT L P+V+  R  Q  KAS +  E  +K  N SN+++DS LKE+++LK ++++D +EK  K +
Sbjct:   18 SCDWPKYLNTLGLCASELVEIRKVLETERFSLAQSLVLTPVRLNPEPDPALAKATDERLPRFDHDTAPQYLRTKLAPQVESQRMAQNSKASAILPEQATKLVNLSNRLLDSGLKEVNALKQELEMDLSEKTFKSS 152
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. TrEMBL
Match: A0A3P7LJM4 (Mediator of RNA polymerase II transcription subunit 8 OS=Dibothriocephalus latus OX=60516 GN=MED8 PE=3 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 2.743e-23
Identity = 58/153 (37.91%), Postives = 90/153 (58.82%), Query Frame = 1 
Query:  112 HEWSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSK-QNASNKVVDSALKELSSLKNQIDVDNTEKAIKQNT-MTEIQELFNCMSNGKGL 564
            ++W KYLN  GL A+EL  I K + +++      LV+ PV LNP PD  L   T +R+  F ++  PQYLRT L P+V+  R  Q  KAS +  E  +K  N SN+++DS LKE+++LK +++ D +EK  K +  + ++  L   ++ G  L
Sbjct:   40 YDWPKYLNTLGLCASELVEIRKVLESERFSLAQALVLTPVRLNPEPDPALAKATDERLPRFDHDTAPQYLRTKLAPQVESQRMAQNAKASAIPPEQATKLVNLSNRLLDSGLKEVNALKQELETDLSEKTFKSSVNLDDLFTLVAAITTGANL 192
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Cavefish
Match: med8 (mediator complex subunit 8 [Source:NCBI gene;Acc:103028217])

HSP 1 Score: 83.1889 bits (204), Expect = 1.052e-18
Identity = 50/154 (32.47%), Postives = 87/154 (56.49%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQ-NASNKVVDSALKELSSLKNQIDVDNTEKAIKQNT----MTEIQELFNCMSNGKGL 564
            W   L+ F LL+ +LN + K +  +K     N V+IP+ L+P+ D++L  LT+ R+  F++E+ P +LRT  DP+V+   K+  ++A+ +  E+  KQ    NK+  S L++L++ ++  D +    AI+QN      T+   L   +  GKGL
Sbjct:   43 WPSVLDNFALLSGQLNTVNKLLRNEKTPSYRNQVIIPLLLSPDRDEDLAKLTEQRVPVFSHEIVPDHLRTKPDPEVEEQEKQLSVEAARIGPEVAQKQIQTMNKLCSSLLEKLNNPRD--DREAETAAIRQNKPSFNPTDTTALVAAVGFGKGL 194
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Sea Lamprey
Match: med8 (mediator complex subunit 8 [Source:NCBI gene;Acc:404615])

HSP 1 Score: 82.4185 bits (202), Expect = 4.189e-19
Identity = 47/152 (30.92%), Postives = 86/152 (56.58%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNAS-NKVVDSALKELSSLKNQIDVDNTEKAIKQNTM--TEIQELFNCMSNGKGL 564
            W   L+ F LL+ +LN++ K +  DK   L   +++P+ L+P+ D  L+ +T+ R+  F+++V P +LRT  DP V+   K+  ++A+ L+ ++  K  AS NK+ +S L  L+S ++  D D++     ++T    +   L   +  GKGL
Sbjct:   42 WPSMLDSFALLSGQLNSLNKLLRNDKTPCLRGQIIMPLLLSPDRDDELMKMTEGRVPVFSHDVVPDHLRTKPDPAVEEQEKQMSLEAARLTPDMAQKHMASMNKMCNSLLDRLNSTRDDRDGDSSTLRHSKHTFNPADTNALVAAVGFGKGL 193
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Nematostella
Match: EDO34499 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SP88])

HSP 1 Score: 76.6406 bits (187), Expect = 6.739e-17
Identity = 47/155 (30.32%), Postives = 88/155 (56.77%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQ-NASNKVVDSALKELSSLKNQIDVD-NTEKAIKQNTMTEIQELFNCMSNGKGLKLSK 576
            W   L+ F LL+ +++ + K +  DK   L NLV++PV +    D+ L+ ++Q RI AF +EV P +LRT  +P+V+ A +  ++ +++++ E   KQ N  N+++ +    ++S K   + + N+      +T  +I  L   ++ GKGLK +K
Sbjct:   42 WPSVLDNFALLSGQVSTLTKLLKGDKTPALRNLVLLPVLVQQEQDEELVKISQGRIPAFNHEVVPDHLRTKYEPEVEEAEQSLVVASASITQENAQKQINHLNELLTTLTDIITSAKEDWEGEQNSASRSTSSTNPDIGALVAAVTFGKGLKPNK 196
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Medaka
Match: med8 (mediator complex subunit 8 [Source:NCBI gene;Acc:101172236])

HSP 1 Score: 77.7962 bits (190), Expect = 1.212e-16
Identity = 48/154 (31.17%), Postives = 85/154 (55.19%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQ-NASNKVVDSALKELSSLKNQIDVDNTEKAIKQNTM----TEIQELFNCMSNGKGL 564
            W   L+ F LL+ +LN I K +  +K     + V+IP+ L+P+ D++L  LT+ R+  F++E+ P +LRT  DP+V+   K+   +A  +  E+  K   A NK+  + L++L++ +   D +    A++QN      T+   L   ++ GKGL
Sbjct:   54 WPSVLDNFALLSGQLNTINKLLRNEKTPAFRSQVIIPLLLSPDRDEDLAKLTEQRVPVFSHEIVPDHLRTKPDPEVEEQEKQLSAEAVRIGPELAQKHIQALNKLCSNLLEKLNNPRE--DREGDASAVRQNKASFNPTDTSALVAAVAFGKGL 205
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Medaka
Match: med8 (mediator complex subunit 8 [Source:NCBI gene;Acc:101172236])

HSP 1 Score: 75.0998 bits (183), Expect = 8.968e-16
Identity = 47/154 (30.52%), Postives = 85/154 (55.19%), Query Frame = 1 
Query:  118 WSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQ-NASNKVVDSALKELSSLKNQIDVDNTEKAIKQNTM----TEIQELFNCMSNGKGL 564
            W   L+ F LL+ +LN I K +  +K     + V+IP+ L+P+ D++L  LT+ R+  F++E+ P +LRT  DP+V+   K+   +A  +  E+  K   A NK+  + L++L++ +   + D    +++QN      T+   L   ++ GKGL
Sbjct:   43 WPSVLDNFALLSGQLNTINKLLRNEKTPAFRSQVIIPLLLSPDRDEDLAKLTEQRVPVFSHEIVPDHLRTKPDPEVEEQEKQLSAEAVRIGPELAQKHIQALNKLCSNLLEKLNNPREDREGD---ASVRQNKASFNPTDTSALVAAVAFGKGL 193
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Planmine SMEST
Match: SMESG000080873.1 (SMESG000080873.1)

HSP 1 Score: 317.39 bits (812), Expect = 3.392e-111
Identity = 156/160 (97.50%), Postives = 159/160 (99.38%), Query Frame = 1 
Query:   97 FRISNHEWSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNASNKVVDSALKELSSLKNQIDVDNTEKAIKQNTMTEIQELFNCMSNGKGLKLSK 576
            F+ ++HEWSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNASNKVVDSALKELSSLKNQIDVDNTEKAIKQNTMTEIQELFNCMSNGKGLKLSK
Sbjct:   30 FQFTDHEWSKYLNIFGLLATELNNIVKTMLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNASNKVVDSALKELSSLKNQIDVDNTEKAIKQNTMTEIQELFNCMSNGKGLKLSK 189

HSP 2 Score: 66.6254 bits (161), Expect = 2.221e-13
Identity = 31/31 (100.00%), Postives = 31/31 (100.00%), Query Frame = 2 
Query:   17 MNKECDLDPLILSLFKFREKVVQLRSCLEFQ 109
            MNKECDLDPLILSLFKFREKVVQLRSCLEFQ
Sbjct:    1 MNKECDLDPLILSLFKFREKVVQLRSCLEFQ 31
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Planmine SMEST
Match: SMESG000080873.1 (SMESG000080873.1)

HSP 1 Score: 265.003 bits (676), Expect = 2.612e-91
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 1 
Query:  181 MLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNASNKVVDSALKELSSLKNQIDVDNTEKAIKQNTMTEIQELFNCMSNGKGLKLSK 576
            MLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNASNKVVDSALKELSSLKNQIDVDNTEKAIKQNTMTEIQELFNCMSNGKGLKLSK
Sbjct:    1 MLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTLLDPKVQHARKEQLIKASNLSAEIVSKQNASNKVVDSALKELSSLKNQIDVDNTEKAIKQNTMTEIQELFNCMSNGKGLKLSK 132
The following BLAST results are available for this feature:
BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
MED81.211e-1530.00mediator complex subunit 8 [Source:HGNC Symbol;Acc... [more]
MED81.630e-1530.00mediator complex subunit 8 [Source:HGNC Symbol;Acc... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MED86.389e-1729.03gene:FBgn0034503 transcript:FBtr0086297[more]
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
med81.090e-1631.17mediator complex subunit 8 [Source:NCBI gene;Acc:4... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
elob2.060e-1630.67elongin B [Source:Xenbase;Acc:XB-GENE-959169][more]
elob2.060e-1630.67elongin B [Source:Xenbase;Acc:XB-GENE-959169][more]
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Med84.214e-1529.33mediator complex subunit 8 [Source:MGI Symbol;Acc:... [more]
Med89.648e-1434.55mediator complex subunit 8 [Source:MGI Symbol;Acc:... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q7Q6D6|MED8_ANOGA6.258e-1731.58Mediator of RNA polymerase II transcription subuni... [more]
sp|A1ZBT5|MED8_DROME6.405e-1629.03Mediator of RNA polymerase II transcription subuni... [more]
sp|Q6NYT1|MED8_DANRE7.822e-1631.17Mediator of RNA polymerase II transcription subuni... [more]
sp|Q6DD30|MED8B_XENLA8.974e-1631.33Mediator of RNA polymerase II transcription subuni... [more]
sp|Q08BU1|MED8_XENTR1.091e-1530.67Mediator of RNA polymerase II transcription subuni... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183A9611.486e-2336.94Mediator of RNA polymerase II transcription subuni... [more]
A0A0X3P6B31.975e-2340.58Mediator of RNA polymerase II transcription subuni... [more]
A0A3P7CF652.171e-2341.48Mediator of RNA polymerase II transcription subuni... [more]
A0A183SJ502.441e-2341.48Mediator of RNA polymerase II transcription subuni... [more]
A0A3P7LJM42.743e-2337.91Mediator of RNA polymerase II transcription subuni... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
med81.052e-1832.47mediator complex subunit 8 [Source:NCBI gene;Acc:1... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
med84.189e-1930.92mediator complex subunit 8 [Source:NCBI gene;Acc:4... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO344996.739e-1730.32Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
med81.212e-1631.17mediator complex subunit 8 [Source:NCBI gene;Acc:1... [more]
med88.968e-1630.52mediator complex subunit 8 [Source:NCBI gene;Acc:1... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 8 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000080873.13.392e-11197.50SMESG000080873.1[more]
SMESG000080873.12.612e-91100.00SMESG000080873.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013672 ID=SMED30013672|Name=Mediator of RNA polymerase II transcription subunit 8|organism=Schmidtea mediterranea sexual|type=transcript|length=708bp
ATTTTTTTACATTGAAATGAACAAAGAATGCGATTTAGATCCATTAATAC
TTTCTCTGTTTAAATTTCGTGAAAAAGTTGTTCAATTACGGAGTTGTTTA
GAATTTCAAATCATGAATGGTCCAAATATTTAAATATATTCGGATTATTG
GCCACCGAATTAAATAACATTGTTAAAACTATGCTGACAGATAAAAATTT
GCAATTAGATAATTTAGTTATGATCCCAGTGGCATTAAATCCTAACCCTG
ACCAGAATCTTATTAATTTAACTCAAGATAGAATCAAAGCGTTTACTTAT
GAAGTACCACCGCAATATCTGCGCACATTGCTGGATCCAAAAGTTCAACA
TGCTAGAAAAGAACAGTTGATAAAAGCTAGTAACTTGTCTGCTGAGATTG
TTTCTAAACAAAACGCGTCAAACAAAGTTGTTGATAGTGCGTTGAAAGAA
TTATCCTCTCTCAAAAATCAAATTGATGTAGATAATACTGAAAAAGCAAT
TAAGCAAAATACAATGACTGAGATTCAGGAACTATTTAATTGCATGTCAA
ATGGGAAAGGATTAAAATTATCGAAATAATATTTTAACTTGTTATTTTTA
TTACCTGTATATGGTTATTTCAAACTATTTGGTGAATGTTATTATGTTTA
GGAAACTGTTTGATTTAGTTTTAAATTGTTAATATTAAGTTTCGATCATC
AAAAAAAA
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protein sequence of SMED30013672-orf-1

>SMED30013672-orf-1 ID=SMED30013672-orf-1|Name=SMED30013672-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=133bp
MLTDKNLQLDNLVMIPVALNPNPDQNLINLTQDRIKAFTYEVPPQYLRTL
LDPKVQHARKEQLIKASNLSAEIVSKQNASNKVVDSALKELSSLKNQIDV
DNTEKAIKQNTMTEIQELFNCMSNGKGLKLSK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003712transcription cofactor activity
GO:0001104RNA polymerase II transcription cofactor activity
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0016592mediator complex
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR019364Mediatior_Med8_fun/met
Vocabulary: biological process
TermDefinition
GO:0006357regulation of transcription from RNA polymerase II promoter
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 84..104
IPR019364Mediator complex, subunit Med8, fungi/metazoaPFAMPF10232Med8coord: 2..130
e-value: 3.2E-23
score: 82.6
IPR019364Mediator complex, subunit Med8, fungi/metazoaPANTHERPTHR13074FAMILY NOT NAMEDcoord: 4..130