DNA directed RNA polymerase III subunit RPC5

Overview
NameDNA directed RNA polymerase III subunit RPC5
Smed IDSMED30013648
Length (bp)2411
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA directed RNA polymerase III subunit RPC5 (SMED30013648) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA directed RNA polymerase III subunit RPC5 (SMED30013648) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA directed RNA polymerase III subunit RPC5 (SMED30013648) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA directed RNA polymerase III subunit RPC5 (SMED30013648) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013648

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30013648SMESG000070822.1 SmedASXL_015262SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30013648SMESG000070822.1 SmedASXL_015262SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Ensembl Zebrafish
Match: polr3e (polymerase (RNA) III (DNA directed) polypeptide E [Source:ZFIN;Acc:ZDB-GENE-030131-8462])

HSP 1 Score: 50.447 bits (119), Expect = 8.401e-6
Identity = 50/191 (26.18%), Postives = 88/191 (46.07%), Query Frame = 2 
Query:  755 REELSDLDEPVKLENRKSICDINPSKTSIVKRSNSVSSMESEILHIFLDCNVISFSKLMESIKKREMVDRSQISEKLVLNYLPTIALLIRGHFVIKSEILY--KFTESDSEIPLENWCLTRDYILSLFYSGEXXXXXXXXXXXXMPSAKITKFLTDLSERMSDSK-NGPWKF-AEHDYDFITKNSIFNQHQ 1315
            RE LS L  P  L   K +  + PS    + +  ++  +  ++  +  +  V+ F+ LM  +        S      VL  +  +ALL++G++V+KS++LY      S S +P E  C  RD+++  F    ++    + +I  +P   +  FL    E+MS  + N  W+F    D DF+ K+    Q Q
Sbjct:  244 REYLSMLMPP--LAEEKVVKPVGPSNVLSMAQLRTLP-LGDQVKTLMKNVKVMPFANLMGLLA-------SGTDSTAVLRCIQQVALLVQGNWVVKSDVLYPKNTCSSHSGVPAEVLCRGRDFVMWRFTIERTLMRKEVAAIIKLPPEDVKDFL----EQMSVPRLNRGWEFLLPTDQDFVKKHPDVAQRQ 420
[Close]
BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Ensembl Zebrafish
Match: polr3e (polymerase (RNA) III (DNA directed) polypeptide E [Source:ZFIN;Acc:ZDB-GENE-030131-8462])

HSP 1 Score: 50.447 bits (119), Expect = 8.401e-6
Identity = 50/191 (26.18%), Postives = 88/191 (46.07%), Query Frame = 2 
Query:  755 REELSDLDEPVKLENRKSICDINPSKTSIVKRSNSVSSMESEILHIFLDCNVISFSKLMESIKKREMVDRSQISEKLVLNYLPTIALLIRGHFVIKSEILY--KFTESDSEIPLENWCLTRDYILSLFYSGEXXXXXXXXXXXXMPSAKITKFLTDLSERMSDSK-NGPWKF-AEHDYDFITKNSIFNQHQ 1315
            RE LS L  P  L   K +  + PS    + +  ++  +  ++  +  +  V+ F+ LM  +        S      VL  +  +ALL++G++V+KS++LY      S S +P E  C  RD+++  F    ++    + +I  +P   +  FL    E+MS  + N  W+F    D DF+ K+    Q Q
Sbjct:  244 REYLSMLMPP--LAEEKVVKPVGPSNVLSMAQLRTLP-LGDQVKTLMKNVKVMPFANLMGLLA-------SGTDSTAVLRCIQQVALLVQGNWVVKSDVLYPKNTCSSHSGVPAEVLCRGRDFVMWRFTIERTLMRKEVAAIIKLPPEDVKDFL----EQMSVPRLNRGWEFLLPTDQDFVKKHPDVAQRQ 420
[Close]
BLAST of DNA directed RNA polymerase III subunit RPC5 vs. TrEMBL
Match: A0A4E0RUJ1 (DNA-directed RNA polymerase III subunit RPC5 OS=Fasciola hepatica OX=6192 GN=D915_003743 PE=4 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.384e-18
Identity = 57/175 (32.57%), Postives = 103/175 (58.86%), Query Frame = 2 
Query:  866 SMESEILHIFLDCNVISFSKLMESIKKREMVDRSQISEKLVLNYLPTIALLIRGHFVIKSEILYKFT--ESDSEIPLENWCLTRDYILSLFYSGEXXXXXXXXXXXXMPSAKITKFLTDLSERMSDSKNG---PWKFAEHDYDFITKNSIFNQHQIEIMDNKVREVCLKLRIDHI 1375
            +ME  +  I L  +VI FSKL+  +++R   D  Q++  LVL ++  +A+L+RG +V+KSE+LY  T       IP       RDYI+++FY GE ++   I S+T +P+   T+ L  L+ +M+ ++ G    W+F   D++FI +     Q Q    + ++R++C++L+++H+
Sbjct:  150 TMEELVQSIMLKVHVIRFSKLVVCVRER-FSDPHQVTNTLVLQHVQKVAVLVRGWWVVKSELLYPPTTYSEHGSIPSTLMIRARDYIMAVFYRGEHLTRKTISSMTKLPALDATEILNLLARKMTSTQKGLANHWEFHPSDHEFIRRFPDVMQQQSAAWEMRIRQLCVQLKLEHL 323
[Close]
BLAST of DNA directed RNA polymerase III subunit RPC5 vs. TrEMBL
Match: G4VNT6 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_142950 PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.480e-16
Identity = 74/224 (33.04%), Postives = 114/224 (50.89%), Query Frame = 2 
Query:  881 ILHIFLDCNVISFSKLMESIKKREMVDRSQISEKLVLNYLPTIALLIRGHFVIKSEILY---KFTESDSEIPLENWCLTRDYILSLFYSGEXXXXXXXXXXXXMPSAKITKFLTDLSERMSDSKNG---PWKFAEHDYDFITKNSIFNQHQIEIMDNKVREVCLKLRIDH-ISKRCRTNSSNSQDTHCSSPGKKRAGDF----------SPSK-MPYKRLRQDS 1498
            I  I +   +I F KL+  +++R   D   ++   VL YLPT A+L+RG +V KSEI+Y    F+E  S  P       RDYI+++F+ GE ++   I  +T +P+ + T+ L  L+ +MS ++ G    WKF   DYDFI K     Q Q    + ++R++C +L+++H +S   R   +  Q  H    G      F          +PSK    KRLRQ S
Sbjct:  260 IQTIMIKAQLIRFDKLVICLRER-FKDPHSVTNTSVLQYLPTFAVLLRGWWVAKSEIIYPPNSFSE-HSGTPSAILIRARDYIMAVFHRGEHLTRKTISCMTKVPTVEATEILKSLACKMSTTQKGCANHWKFYPTDYDFIKKYPDIVQQQQVAWEIRIRQLCTQLKLEHLVSDGVRRKRNPGQ--HSGESGSDSDTGFKSLVSGVHISTPSKNTNRKRLRQSS 479
[Close]
BLAST of DNA directed RNA polymerase III subunit RPC5 vs. TrEMBL
Match: A0A3Q0KNL9 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.602e-16
Identity = 74/224 (33.04%), Postives = 114/224 (50.89%), Query Frame = 2 
Query:  881 ILHIFLDCNVISFSKLMESIKKREMVDRSQISEKLVLNYLPTIALLIRGHFVIKSEILY---KFTESDSEIPLENWCLTRDYILSLFYSGEXXXXXXXXXXXXMPSAKITKFLTDLSERMSDSKNG---PWKFAEHDYDFITKNSIFNQHQIEIMDNKVREVCLKLRIDH-ISKRCRTNSSNSQDTHCSSPGKKRAGDF----------SPSK-MPYKRLRQDS 1498
            I  I +   +I F KL+  +++R   D   ++   VL YLPT A+L+RG +V KSEI+Y    F+E  S  P       RDYI+++F+ GE ++   I  +T +P+ + T+ L  L+ +MS ++ G    WKF   DYDFI K     Q Q    + ++R++C +L+++H +S   R   +  Q  H    G      F          +PSK    KRLRQ S
Sbjct:  260 IQTIMIKAQLIRFDKLVICLRER-FKDPHSVTNTSVLQYLPTFAVLLRGWWVAKSEIIYPPNSFSE-HSGTPSAILIRARDYIMAVFHRGEHLTRKTISCMTKVPTVEATEILKSLACKMSTTQKGCANHWKFYPTDYDFIKKYPDIVQQQQVAWEIRIRQLCTQLKLEHLVSDGVRRKRNPGQ--HSGESGSDSDTGFKSLVSGVHISTPSKNTNRKRLRQSS 479
[Close]
BLAST of DNA directed RNA polymerase III subunit RPC5 vs. TrEMBL
Match: A0A4S2LIN5 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_007280 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 6.622e-15
Identity = 53/175 (30.29%), Postives = 100/175 (57.14%), Query Frame = 2 
Query:  866 SMESEILHIFLDCNVISFSKLMESIKKREMVDRSQISEKLVLNYLPTIALLIRGHFVIKSEILYKFT--ESDSEIPLENWCLTRDYILSLFYSGEXXXXXXXXXXXXMPSAKITKFLTDLSERMSDSKNG---PWKFAEHDYDFITKNSIFNQHQIEIMDNKVREVCLKLRIDHI 1375
            +ME  +  I +  +VI F+KL+  +++R   D   ++   VL +L  +A+LIRG +V+KSE+LY  T     + +P       RDYIL++F+ GE ++   I S+T +P+ + T+ L  ++ +M+ ++ G    W+F   D DF+ K     Q Q    + ++R++C +L+++ +
Sbjct:  248 TMEELLQSIMMKVHVIRFNKLVACVRER-FSDPHSVTNAAVLQHLQKVAVLIRGWWVVKSELLYPPTMYSEHAAVPSSLMIRARDYILAVFHRGEHLTRKTISSMTKLPALEATEILKLVARKMNATQKGHVNHWEFHPLDTDFVRKYPDVVQQQHLTWEARIRQLCGQLKLERL 421
[Close]
BLAST of DNA directed RNA polymerase III subunit RPC5 vs. TrEMBL
Match: A0A4S2LGI1 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_007280 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 6.762e-15
Identity = 53/175 (30.29%), Postives = 100/175 (57.14%), Query Frame = 2 
Query:  866 SMESEILHIFLDCNVISFSKLMESIKKREMVDRSQISEKLVLNYLPTIALLIRGHFVIKSEILYKFT--ESDSEIPLENWCLTRDYILSLFYSGEXXXXXXXXXXXXMPSAKITKFLTDLSERMSDSKNG---PWKFAEHDYDFITKNSIFNQHQIEIMDNKVREVCLKLRIDHI 1375
            +ME  +  I +  +VI F+KL+  +++R   D   ++   VL +L  +A+LIRG +V+KSE+LY  T     + +P       RDYIL++F+ GE ++   I S+T +P+ + T+ L  ++ +M+ ++ G    W+F   D DF+ K     Q Q    + ++R++C +L+++ +
Sbjct:  246 TMEELLQSIMMKVHVIRFNKLVACVRER-FSDPHSVTNAAVLQHLQKVAVLIRGWWVVKSELLYPPTMYSEHAAVPSSLMIRARDYILAVFHRGEHLTRKTISSMTKLPALEATEILKLVARKMNATQKGHVNHWEFHPLDTDFVRKYPDVVQQQHLTWEARIRQLCGQLKLERL 419
[Close]
BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Planmine SMEST
Match: SMESG000070822.1 (SMESG000070822.1)

HSP 1 Score: 1022.31 bits (2642), Expect = 0.000e+0
Identity = 544/551 (98.73%), Postives = 547/551 (99.27%), Query Frame = 2 
Query:  689 IVIMRY*NNITECQQSLLLCYEREELSDLDEPVKLENRKSICDINPSKTSIVKRSNSVSSMESEILHIFLDCNVISFSKLMESIKKREMVDRSQISEKLVLNYLPTIALLIRGHFVIKSEILYKFTESDSEIPLENWCLTRDYILSLFYSGEXXXXXXXXXXXXMPSAKITKFLTDLSERMSDSKNGPWKFAEHDYDFITKNSIFNQHQIEIMDNKVREVCLKLRIDHISKRCRTNSSNSQDTHCSSPGKKRAGDFSPSKMPYKRLRQDSSGNESLENCNSKITTVVNEHYTKLSIAQGSETLINTKEHLSASKSVVMNCDFPLIKGEPCMSPRESPDREYILDNETYSIIKTEANDNISEKENMKKKVLXXXXXXXXXXXXXXIPEILLDDIKQFXXXXXXXXXXXPIRNLAKTFQEEVLIKLPVYKSGKCSGLTNDSLTRQIVETMEKILTELQVTKIDIKWPSLKTALEPQPIYLSSNESGENVKIFSAVIHLLNTQGYCSLNDVKNEITARKTMELIPNNNTLRDKLKKFCNYVHRKYYLKFTVSDR 2341
            +V+ R  NNITECQQSLLLCYEREELSDLDEPVKLENRKSICDINPSKTSIVKRSNSVSSMESEILHIFLDCNVISFSKLMESIKKREMVDRSQISEKLVLNYLPTIALLIRGHFVIKSEILYKFTESDSEIPLENWCLTRDYILSLFYSGESISTTAIKSITSMPSAKITKFLTDLSERMSDSKNGPWKFAEHDYDFITKNSIFNQHQIEIMDNKVREVCLKLRIDHISKRCRTNSSNSQDTHCSSPGKKRAGDFSPSKMPYKRLRQDSSGNESLENCNSKITTVVNEHYTKLSIAQGSETLINTKEHLSASKSVVMNCDFPLIKGEPCMSPRESPDREYIL+NETYSIIKTEANDNISEKENMKKKVLTNSRETTTTEQTNQIPEILLDDIKQFLLLQLSSSDLLPIRNLAKTFQEEVLIKLPVYKSGKCSGLTNDSLTR IVETMEKILTELQVTKIDIKWPSLKTALEPQPIYLSSNESGENVKIFSAVIHLLNTQGYCSLNDVKNEITARKTMELIPNNNTLRDKLKKFCNYVHRKYYLKFTVSDR
Sbjct:  118 VVLKRSNNNITECQQSLLLCYEREELSDLDEPVKLENRKSICDINPSKTSIVKRSNSVSSMESEILHIFLDCNVISFSKLMESIKKREMVDRSQISEKLVLNYLPTIALLIRGHFVIKSEILYKFTESDSEIPLENWCLTRDYILSLFYSGESISTTAIKSITSMPSAKITKFLTDLSERMSDSKNGPWKFAEHDYDFITKNSIFNQHQIEIMDNKVREVCLKLRIDHISKRCRTNSSNSQDTHCSSPGKKRAGDFSPSKMPYKRLRQDSSGNESLENCNSKITTVVNEHYTKLSIAQGSETLINTKEHLSASKSVVMNCDFPLIKGEPCMSPRESPDREYILENETYSIIKTEANDNISEKENMKKKVLTNSRETTTTEQTNQIPEILLDDIKQFLLLQLSSSDLLPIRNLAKTFQEEVLIKLPVYKSGKCSGLTNDSLTRPIVETMEKILTELQVTKIDIKWPSLKTALEPQPIYLSSNESGENVKIFSAVIHLLNTQGYCSLNDVKNEITARKTMELIPNNNTLRDKLKKFCNYVHRKYYLKFTVSDR 668
The following BLAST results are available for this feature:
BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
polr3e8.401e-626.18polymerase (RNA) III (DNA directed) polypeptide E ... [more]
polr3e8.401e-626.18polymerase (RNA) III (DNA directed) polypeptide E ... [more]
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BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA directed RNA polymerase III subunit RPC5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA directed RNA polymerase III subunit RPC5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4E0RUJ11.384e-1832.57DNA-directed RNA polymerase III subunit RPC5 OS=Fa... [more]
G4VNT61.480e-1633.04Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A3Q0KNL91.602e-1633.04Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A4S2LIN56.622e-1530.29Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4S2LGI16.762e-1530.29Uncharacterized protein OS=Opisthorchis felineus O... [more]
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BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA directed RNA polymerase III subunit RPC5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000070822.10.000e+098.73SMESG000070822.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013648 ID=SMED30013648|Name=DNA directed RNA polymerase III subunit RPC5|organism=Schmidtea mediterranea sexual|type=transcript|length=2411bp
CAGCTTAGAAAGCCAGGATATTTAAGCACGTTCAATTTCTCTACTTATTT
CGGAGTTTGTATCAATGCAATGCCCCCTCCCCCCGTTCTCGAACTCCTAT
ATTCCCCTTTTCACTAACTCAATAATTGGAATATGATCGTATTAATGCTC
ATGAAATCCACCACGGTATCGCAAGAATGAATGCAATTGATATACATTCC
TCTGTAACATGTCCGGAACCCTGCTAAATTTATATGATTGGTATAAAAGT
CAAATCAAAGAACTCCATCACTCTGGATTTGTGGACTTCTGAAACTAATT
CCTATTTGAGTCTCACAACACATTGGATAAAAATTGCAATGCTTCACCGC
CAGAGGTTTGAAGGGCAGCACACTGCAGATCCCATTTGTCACTGCCACCT
TGTTCTACGCACAAGCAATATCAGTAAGGCTTTTCGTGACATTGTCGCTG
ATAACTTTCTGCATGCAATCATAGTAACCGTTGGACATTGTTATGATTTC
CCGGAACCGTTTACAACATTTCATTTAATCTTCCCTGGCTACAAATCATC
CACATCAAATCCAACTTTCTGAAACTTCACCTTTTTTGTTGGAACCAAAT
CGGCTTGTTACTCTTTATTATACTTTAACGTTTTTTGGTTTTTATTATCC
TCTTATAAAACATGCTGATTCATTGGCTTTTCAACTAAATTGTAATAATG
CGATATTAGAATAACATCACAGAATGCCAACAGTCACTGTTGTTATGTTA
CGAGCGAGAGGAACTGAGTGATTTGGATGAGCCGGTAAAATTGGAGAACC
GAAAATCTATCTGTGATATTAATCCCAGCAAAACATCCATCGTTAAAAGA
TCCAATTCAGTTTCGTCAATGGAGTCAGAAATTTTGCACATTTTTCTCGA
TTGCAATGTAATTTCATTTTCAAAACTGATGGAGTCCATTAAAAAACGAG
AAATGGTCGACCGAAGTCAGATCTCAGAGAAACTTGTTCTTAATTATCTC
CCGACAATTGCACTTTTAATCCGCGGTCATTTTGTTATCAAAAGTGAAAT
ACTTTACAAATTTACCGAATCTGACAGTGAAATACCATTGGAAAATTGGT
GCTTGACTCGAGATTATATTCTATCACTATTCTACAGTGGGGAAAGTATC
AGTACGACTGCTATCAAGTCGATTACATCAATGCCTTCAGCAAAAATTAC
TAAATTTCTTACTGATTTATCTGAAAGAATGAGTGATTCCAAAAATGGTC
CATGGAAATTTGCTGAACATGATTATGATTTTATAACAAAAAATAGCATT
TTTAATCAGCACCAAATTGAAATAATGGACAACAAAGTCAGAGAAGTTTG
TTTGAAATTAAGAATTGACCATATCAGCAAAAGATGTAGAACAAACTCAT
CCAACTCACAGGATACCCATTGTTCAAGTCCGGGTAAAAAACGAGCTGGA
GATTTTTCACCGTCAAAAATGCCTTATAAAAGATTACGCCAAGATAGTAG
TGGAAATGAATCACTGGAAAATTGCAATTCAAAAATAACAACTGTAGTAA
ATGAGCATTATACTAAATTGTCTATTGCACAGGGTTCCGAAACCTTAATT
AACACTAAAGAACATTTATCCGCTTCGAAATCAGTCGTTATGAATTGCGA
TTTCCCCCTTATAAAAGGTGAACCATGTATGTCTCCTCGGGAGTCTCCAG
ATAGAGAATACATTCTAGACAACGAAACTTATTCCATAATTAAAACCGAA
GCCAACGATAACATCTCAGAAAAAGAAAACATGAAGAAAAAAGTTTTAAC
CAACTCGCGTGAAACGACAACCACTGAACAAACAAACCAAATTCCTGAAA
TTTTATTGGATGATATTAAACAGTTTTTGCTGCTTCAGTTGTCCAGTTCG
GATTTGCTGCCGATAAGAAATTTAGCCAAAACCTTTCAAGAGGAAGTACT
TATTAAGCTGCCTGTTTACAAGTCGGGTAAATGCAGTGGCTTAACCAATG
ATTCTTTGACCAGACAAATTGTCGAAACCATGGAAAAGATATTGACAGAA
CTGCAGGTCACAAAAATTGATATAAAGTGGCCCTCTCTGAAGACCGCCTT
GGAACCGCAGCCGATTTATCTCTCTAGTAATGAATCCGGTGAAAACGTTA
AAATCTTCAGTGCGGTGATACATTTACTGAACACTCAAGGCTACTGTAGC
CTCAACGACGTGAAAAACGAAATCACCGCCCGCAAAACGATGGAGCTAAT
TCCAAATAACAATACGCTGAGAGACAAATTAAAAAAATTCTGTAATTATG
TCCATCGAAAATATTATTTGAAATTTACAGTTTCCGATCGATAAATGTTT
ATTAATTTTTATTCGATTTATTATTTTAATTATGAATAAATTCTCCTTCA
ATCAAAAAAAA
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protein sequence of SMED30013648-orf-1

>SMED30013648-orf-1 ID=SMED30013648-orf-1|Name=SMED30013648-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=492bp
MESEILHIFLDCNVISFSKLMESIKKREMVDRSQISEKLVLNYLPTIALL
IRGHFVIKSEILYKFTESDSEIPLENWCLTRDYILSLFYSGESISTTAIK
SITSMPSAKITKFLTDLSERMSDSKNGPWKFAEHDYDFITKNSIFNQHQI
EIMDNKVREVCLKLRIDHISKRCRTNSSNSQDTHCSSPGKKRAGDFSPSK
MPYKRLRQDSSGNESLENCNSKITTVVNEHYTKLSIAQGSETLINTKEHL
SASKSVVMNCDFPLIKGEPCMSPRESPDREYILDNETYSIIKTEANDNIS
EKENMKKKVLTNSRETTTTEQTNQIPEILLDDIKQFLLLQLSSSDLLPIR
NLAKTFQEEVLIKLPVYKSGKCSGLTNDSLTRQIVETMEKILTELQVTKI
DIKWPSLKTALEPQPIYLSSNESGENVKIFSAVIHLLNTQGYCSLNDVKN
EITARKTMELIPNNNTLRDKLKKFCNYVHRKYYLKFTVSDR*
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protein sequence of SMED30013648-orf-2

>SMED30013648-orf-2 ID=SMED30013648-orf-2|Name=SMED30013648-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=113bp
MIGIKVKSKNSITLDLWTSETNSYLSLTTHWIKIAMLHRQRFEGQHTADP
ICHCHLVLRTSNISKAFRDIVADNFLHAIIVTVGHCYDFPEPFTTFHLIF
PGYKSSTSNPTF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR006886RNA_pol_III_Rpc5
Vocabulary: biological process
TermDefinition
GO:0006351transcription, DNA-templated
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
Vocabulary: molecular function
TermDefinition
GO:0003899DNA-directed 5'-3' RNA polymerase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006886DNA-directed RNA polymerase III subunit Rpc5PFAMPF04801Sin_Ncoord: 4..151
e-value: 5.9E-23
score: 81.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 175..215