SMED30013634

Overview
NameSMED30013634
Smed IDSMED30013634
Length (bp)1542
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30013634 (SMED30013634) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30013634 (SMED30013634) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30013634 (SMED30013634) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30013634 (SMED30013634) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013634

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30013634 dd_Smed_v4_14761_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30013634 dd_Smed_v4_14761_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30013634 dd_Smed_v4_14761_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30013634 dd_Smed_v4_14761_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30013634 dd_Smed_v4_14761_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30013634 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.010573:24939..26455 -6646
Homology
[Close]
BLAST of SMED30013634 vs. Ensembl Human
Match: PIF1 (PIF1 5'-to-3' DNA helicase [Source:HGNC Symbol;Acc:HGNC:26220])

HSP 1 Score: 53.5286 bits (127), Expect = 8.299e-7
Identity = 42/128 (32.81%), Postives = 66/128 (51.56%), Query Frame = -1 
Query:  619 LTSKCNSPHKLVLKIGSLVMLLRNLDPPQ-IRNGSRLTV---KALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAK 990
            L ++C     L LK+G+ VML++NL   + + NG+R  V   +A    + +   L G  E  HA    +      L     R Q P+Q+A A +I+K QG +L+   + L    F++GQ YV +SRA+
Sbjct:  463 LDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQL---LSRQQLPLQLAWAMSIHKSQGMTLDCVEISL-GRVFASGQAYVALSRAR 586
[Close]
BLAST of SMED30013634 vs. Ensembl Human
Match: PIF1 (PIF1 5'-to-3' DNA helicase [Source:HGNC Symbol;Acc:HGNC:26220])

HSP 1 Score: 53.1434 bits (126), Expect = 8.761e-7
Identity = 42/128 (32.81%), Postives = 66/128 (51.56%), Query Frame = -1 
Query:  619 LTSKCNSPHKLVLKIGSLVMLLRNLDPPQ-IRNGSRLTV---KALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAK 990
            L ++C     L LK+G+ VML++NL   + + NG+R  V   +A    + +   L G  E  HA    +      L     R Q P+Q+A A +I+K QG +L+   + L    F++GQ YV +SRA+
Sbjct:  463 LDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQL---LSRQQLPLQLAWAMSIHKSQGMTLDCVEISL-GRVFASGQAYVALSRAR 586
[Close]
BLAST of SMED30013634 vs. Ensembl Human
Match: PIF1 (PIF1 5'-to-3' DNA helicase [Source:HGNC Symbol;Acc:HGNC:26220])

HSP 1 Score: 53.1434 bits (126), Expect = 8.761e-7
Identity = 42/128 (32.81%), Postives = 66/128 (51.56%), Query Frame = -1 
Query:  619 LTSKCNSPHKLVLKIGSLVMLLRNLDPPQ-IRNGSRLTV---KALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAK 990
            L ++C     L LK+G+ VML++NL   + + NG+R  V   +A    + +   L G  E  HA    +      L     R Q P+Q+A A +I+K QG +L+   + L    F++GQ YV +SRA+
Sbjct:  463 LDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQL---LSRQQLPLQLAWAMSIHKSQGMTLDCVEISL-GRVFASGQAYVALSRAR 586
[Close]
BLAST of SMED30013634 vs. Ensembl Celegans
Match: Y46B2A.2 (ATP-dependent DNA helicase [Source:UniProtKB/TrEMBL;Acc:Q9N4X4])

HSP 1 Score: 89.3521 bits (220), Expect = 2.074e-18
Identity = 48/121 (39.67%), Postives = 73/121 (60.33%), Query Frame = -1 
Query:  613 PHKLVLKIGSLVMLLRNLD-PPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFST-DLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAKDK 969
            PH L LK GS+++LLRNLD    + NGSR  V+ L ++ +      G  +G   +I RI  +   ++ F  +R QFP++++ A +INK QGQS    GL +  + F++GQLYV +SR + K
Sbjct: 1224 PHILKLKKGSVIILLRNLDVSAGLCNGSRFIVETLASHSLGCRFATGERKGHFTIIPRIDCYDDKNISFQLRRTQFPVRLSFALSINKAQGQSFSKIGLWIPTDVFTHGQLYVALSRVRTK 1344
[Close]
BLAST of SMED30013634 vs. Ensembl Celegans
Match: F33H12.6 (ATP-dependent DNA helicase [Source:UniProtKB/TrEMBL;Acc:O44843])

HSP 1 Score: 89.3521 bits (220), Expect = 2.108e-18
Identity = 48/121 (39.67%), Postives = 73/121 (60.33%), Query Frame = -1 
Query:  613 PHKLVLKIGSLVMLLRNLD-PPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFST-DLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAKDK 969
            PH L LK GS+++LLRNLD    + NGSR  V+ L ++ +      G  +G   +I RI  +   ++ F  +R QFP++++ A +INK QGQS    GL +  + F++GQLYV +SR + K
Sbjct: 1219 PHILKLKKGSVIILLRNLDVSAGLCNGSRFIVETLASHSLGCRFATGERKGHFTIIPRIDCYDDKNISFQLRRTQFPVRLSFALSINKAQGQSFSKIGLWIPTDVFTHGQLYVALSRVRTK 1339
[Close]
BLAST of SMED30013634 vs. Ensembl Celegans
Match: F59H6.5 (ATP-dependent DNA helicase [Source:UniProtKB/TrEMBL;Acc:Q9N5Q7])

HSP 1 Score: 89.3521 bits (220), Expect = 2.127e-18
Identity = 48/121 (39.67%), Postives = 73/121 (60.33%), Query Frame = -1 
Query:  613 PHKLVLKIGSLVMLLRNLD-PPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFST-DLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAKDK 969
            PH L LK GS+++LLRNLD    + NGSR  V+ L ++ +      G  +G   +I RI  +   ++ F  +R QFP++++ A +INK QGQS    GL +  + F++GQLYV +SR + K
Sbjct: 1345 PHILKLKKGSVIILLRNLDVSAGLCNGSRFIVETLASHSLGCRFATGERKGHFTIIPRIDCYDDKNISFQLRRTQFPVRLSFALSINKAQGQSFSKIGLWIPTDVFTHGQLYVALSRVRTK 1465
[Close]
BLAST of SMED30013634 vs. Ensembl Celegans
Match: ZK250.9 (ATP-dependent DNA helicase [Source:UniProtKB/TrEMBL;Acc:G5ED32])

HSP 1 Score: 89.3521 bits (220), Expect = 2.157e-18
Identity = 48/121 (39.67%), Postives = 73/121 (60.33%), Query Frame = -1 
Query:  613 PHKLVLKIGSLVMLLRNLD-PPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFST-DLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAKDK 969
            PH L LK GS+++LLRNLD    + NGSR  V+ L ++ +      G  +G   +I RI  +   ++ F  +R QFP++++ A +INK QGQS    GL +  + F++GQLYV +SR + K
Sbjct: 1325 PHILKLKKGSVIILLRNLDVSAGLCNGSRFIVETLASHSLGCRFATGERKGHFTIIPRIDCYDDKNISFQLRRTQFPVRLSFALSINKAQGQSFSKIGLWIPTDVFTHGQLYVALSRVRTK 1445
[Close]
BLAST of SMED30013634 vs. Ensembl Celegans
Match: Y16E11A.2 (ATP-dependent DNA helicase [Source:UniProtKB/TrEMBL;Acc:G5ED32])

HSP 1 Score: 89.3521 bits (220), Expect = 2.157e-18
Identity = 48/121 (39.67%), Postives = 73/121 (60.33%), Query Frame = -1 
Query:  613 PHKLVLKIGSLVMLLRNLD-PPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFST-DLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAKDK 969
            PH L LK GS+++LLRNLD    + NGSR  V+ L ++ +      G  +G   +I RI  +   ++ F  +R QFP++++ A +INK QGQS    GL +  + F++GQLYV +SR + K
Sbjct: 1325 PHILKLKKGSVIILLRNLDVSAGLCNGSRFIVETLASHSLGCRFATGERKGHFTIIPRIDCYDDKNISFQLRRTQFPVRLSFALSINKAQGQSFSKIGLWIPTDVFTHGQLYVALSRVRTK 1445
[Close]
BLAST of SMED30013634 vs. Ensembl Fly
Match: Pif1 (gene:FBgn0031540 transcript:FBtr0077569)

HSP 1 Score: 49.2914 bits (116), Expect = 8.336e-6
Identity = 39/126 (30.95%), Postives = 65/126 (51.59%), Query Frame = -1 
Query:  619 LTSKCNSPHKLVLKIGSLVMLLRNLDPPQ-IRNGSR-LTVKALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAK 990
            L  +  +P +L LK+ + VMLL+N++    + NG+R + V+   +  +     N  Y  KH       +  T       R Q P+++A A +I+K QG +L+   + L+ + F  GQ YV +SRAK
Sbjct:  469 LDQQIQAPSQLYLKVNAQVMLLKNINISNGLVNGARGVVVRMEKDLPVVRFKNNQEYVCKHERW----IIKTASGNHITRRQVPLKLAWAFSIHKSQGLTLDCVEMSLS-KVFEAGQAYVALSRAK 589
[Close]
BLAST of SMED30013634 vs. Ensembl Fly
Match: Pif1 (gene:FBgn0031540 transcript:FBtr0343239)

HSP 1 Score: 49.2914 bits (116), Expect = 8.336e-6
Identity = 39/126 (30.95%), Postives = 65/126 (51.59%), Query Frame = -1 
Query:  619 LTSKCNSPHKLVLKIGSLVMLLRNLDPPQ-IRNGSR-LTVKALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAK 990
            L  +  +P +L LK+ + VMLL+N++    + NG+R + V+   +  +     N  Y  KH       +  T       R Q P+++A A +I+K QG +L+   + L+ + F  GQ YV +SRAK
Sbjct:  469 LDQQIQAPSQLYLKVNAQVMLLKNINISNGLVNGARGVVVRMEKDLPVVRFKNNQEYVCKHERW----IIKTASGNHITRRQVPLKLAWAFSIHKSQGLTLDCVEMSLS-KVFEAGQAYVALSRAK 589
[Close]
BLAST of SMED30013634 vs. UniProt/SwissProt
Match: sp|Q9H611|PIF1_HUMAN (ATP-dependent DNA helicase PIF1 OS=Homo sapiens OX=9606 GN=PIF1 PE=1 SV=2)

HSP 1 Score: 53.1434 bits (126), Expect = 4.207e-6
Identity = 42/128 (32.81%), Postives = 66/128 (51.56%), Query Frame = -1 
Query:  619 LTSKCNSPHKLVLKIGSLVMLLRNLDPPQ-IRNGSRLTV---KALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAK 990
            L ++C     L LK+G+ VML++NL   + + NG+R  V   +A    + +   L G  E  HA    +      L     R Q P+Q+A A +I+K QG +L+   + L    F++GQ YV +SRA+
Sbjct:  463 LDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQL---LSRQQLPLQLAWAMSIHKSQGMTLDCVEISL-GRVFASGQAYVALSRAR 586
[Close]
BLAST of SMED30013634 vs. TrEMBL
Match: A0A4Y2I5Q6 (ATP-dependent DNA helicase OS=Araneus ventricosus OX=182803 GN=pif1_31 PE=3 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 2.840e-67
Identity = 93/147 (63.27%), Postives = 113/147 (76.87%), Query Frame = -1 
Query:  550 LTSKCNSPHKLVLKIGSLVMLLRNLDPPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAKDKNWKPKMLDMTKY*NNINYNAI 990
            L   C   H+LVLK+G  +MLLRNLDPP++ NGSRL VKAL+ ++I+ATIL GP+EG+H LI RIPL  TDLPFSFQRLQFP+++A A TINK QGQSL VTGLDLT+E FS+GQLYVGMSRAKD +    + D  K   NI Y+ +
Sbjct:  528 LNPSCIPLHRLVLKVGCPIMLLRNLDPPKLCNGSRLIVKALHAHIIEATILTGPFEGEHVLIPRIPLIPTDLPFSFQRLQFPLRLAFAITINKAQGQSLRVTGLDLTDECFSHGQLYVGMSRAKDTSTLFVIADEQKETKNIVYSQV 674

HSP 2 Score: 98.9821 bits (245), Expect = 2.840e-67
Identity = 50/94 (53.19%), Postives = 67/94 (71.28%), Query Frame = -2 
Query:  969 KLFNKFGNICQNLNDMINCVYPNADANPFDWKWLQERAMLAPLNVIVEEINYYIQEKIQNNFQTF*SIYKCVNKDEATSYPVEVFNTLHQSVIP 1250
            +L N+F  IC  L ++IN VYPN   N  + +WLQERA+LAPLN  V+EIN+ +QEK+    +T+ SI KC+N +EATSYPVE  N+L+ S IP
Sbjct:  441 RLSNEFCKICPTLENLINQVYPNITLNIQNTQWLQERAILAPLNEKVKEINFTVQEKVPTAARTYYSIDKCLNDEEATSYPVEFLNSLNPSCIP 534
[Close]
BLAST of SMED30013634 vs. TrEMBL
Match: A0A4Y2LYL7 (ATP-dependent DNA helicase (Fragment) OS=Araneus ventricosus OX=182803 GN=pif1_137 PE=3 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 5.267e-67
Identity = 92/147 (62.59%), Postives = 113/147 (76.87%), Query Frame = -1 
Query:  550 LTSKCNSPHKLVLKIGSLVMLLRNLDPPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAKDKNWKPKMLDMTKY*NNINYNAI 990
            L   C   H+LVLK+G  +MLLRNLDPP++ NGSRL VKAL+ ++I+ATIL GP+EG+H LI R+PL  TDLPFSFQRLQFP+++A A TINK QGQSL VTGLDLT+E FS+GQLYVGMSRAKD +    + D  K   NI Y+ +
Sbjct: 1189 LNPSCIPLHRLVLKVGCPIMLLRNLDPPKLCNGSRLIVKALHAHIIEATILTGPFEGEHVLIPRVPLIPTDLPFSFQRLQFPLRLAFAITINKAQGQSLRVTGLDLTDECFSHGQLYVGMSRAKDTSTLFIIADEQKETKNIVYSQV 1335

HSP 2 Score: 99.7525 bits (247), Expect = 5.267e-67
Identity = 50/94 (53.19%), Postives = 68/94 (72.34%), Query Frame = -2 
Query:  969 KLFNKFGNICQNLNDMINCVYPNADANPFDWKWLQERAMLAPLNVIVEEINYYIQEKIQNNFQTF*SIYKCVNKDEATSYPVEVFNTLHQSVIP 1250
            +L N+F  IC  L ++IN VYP+   N  + +WLQERA+LAPLN  V+EIN+ +QEK+Q   +T+ SI KC+N +EATSYPVE  N+L+ S IP
Sbjct: 1102 RLSNEFCKICPTLENLINQVYPDITLNIQNIQWLQERAILAPLNEKVKEINFTVQEKVQTAARTYYSIDKCLNDEEATSYPVEFLNSLNPSCIP 1195

HSP 3 Score: 65.0846 bits (157), Expect = 3.529e-7
Identity = 30/46 (65.22%), Postives = 38/46 (82.61%), Query Frame = -2 
Query: 1404 GYSIYIN*IQPRTSNKVNKMVSAMPYYAYRLMVRPNEN*QLNYRKI 1541
            GY+I IN ++P TSN++NKMVSAM +YAYRLMVR  EN  LNYR++
Sbjct:  278 GYTININQVEPGTSNQINKMVSAMSFYAYRLMVRSTENHLLNYRQL 323
[Close]
BLAST of SMED30013634 vs. TrEMBL
Match: A0A4Y2K3R1 (ATP-dependent DNA helicase OS=Araneus ventricosus OX=182803 GN=pif1_145 PE=3 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 1.521e-66
Identity = 93/147 (63.27%), Postives = 113/147 (76.87%), Query Frame = -1 
Query:  550 LTSKCNSPHKLVLKIGSLVMLLRNLDPPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAKDKNWKPKMLDMTKY*NNINYNAI 990
            L   C   H+LVLK+G  +MLLRNLDPP++ NGSRL VKAL+ ++I+ATIL GP+EG+H LI RIPL  TDLPFSFQRLQFP+++A A TINK QGQSL VTGLDLT+E FS+GQLYVGMSRAKD +    + D  K   NI Y+ +
Sbjct:  391 LNPSCIPLHRLVLKVGCPIMLLRNLDPPKLCNGSRLIVKALHAHIIEATILTGPFEGEHVLILRIPLIPTDLPFSFQRLQFPLRLAFAITINKAQGQSLRVTGLDLTDECFSHGQLYVGMSRAKDTSTLFIIADEQKETKNIVYSQV 537

HSP 2 Score: 96.2857 bits (238), Expect = 1.521e-66
Identity = 48/94 (51.06%), Postives = 66/94 (70.21%), Query Frame = -2 
Query:  969 KLFNKFGNICQNLNDMINCVYPNADANPFDWKWLQERAMLAPLNVIVEEINYYIQEKIQNNFQTF*SIYKCVNKDEATSYPVEVFNTLHQSVIP 1250
            +L N+F  IC  L ++ N VYP+   N  + +WLQERA+LAPLN  V+EIN+ +QEK+    +T+ SI KC+N +EATSYPVE  N+L+ S IP
Sbjct:  304 RLSNEFCKICPTLENLTNQVYPDITLNIQNIQWLQERAILAPLNEKVQEINFTVQEKVPTAARTYYSIDKCLNDEEATSYPVEFLNSLNPSCIP 397
[Close]
BLAST of SMED30013634 vs. TrEMBL
Match: A0A4Y2CDA4 (ATP-dependent DNA helicase OS=Araneus ventricosus OX=182803 GN=pif1_20 PE=3 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 2.525e-66
Identity = 93/147 (63.27%), Postives = 113/147 (76.87%), Query Frame = -1 
Query:  550 LTSKCNSPHKLVLKIGSLVMLLRNLDPPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAKDKNWKPKMLDMTKY*NNINYNAI 990
            L   C   H+LVLK+G  +MLLRNLDPP++ NGSRL VKAL+ ++I+ATIL GP+EG+H LI RIPL  TDLPFSFQRLQFP+++A A TINK QGQSL VTGLDLT+E FS+GQLYVGMSRAKD +    + D  K   NI Y+ +
Sbjct: 1340 LNPSCIPLHRLVLKVGCPIMLLRNLDPPKLCNGSRLIVKALHAHIIEATILTGPFEGEHVLIPRIPLIPTDLPFSFQRLQFPLRLAFAITINKAQGQSLRVTGLDLTDECFSHGQLYVGMSRAKDTSTLFIIADEQKETKNIVYSQV 1486

HSP 2 Score: 97.0561 bits (240), Expect = 2.525e-66
Identity = 49/94 (52.13%), Postives = 67/94 (71.28%), Query Frame = -2 
Query:  969 KLFNKFGNICQNLNDMINCVYPNADANPFDWKWLQERAMLAPLNVIVEEINYYIQEKIQNNFQTF*SIYKCVNKDEATSYPVEVFNTLHQSVIP 1250
            +L N+F  IC  L ++IN VYP+   N  + +WLQERA+LAPLN  V+EIN+ +QEK+    +T+ SI KC+N +EATSYPVE  N+L+ S IP
Sbjct: 1253 RLSNEFCKICPTLENLINQVYPDITLNIQNIQWLQERAILAPLNEKVKEINFTVQEKVPTAARTYYSIDKCLNDEEATSYPVEFLNSLNPSCIP 1346

HSP 3 Score: 64.6994 bits (156), Expect = 5.054e-7
Identity = 30/46 (65.22%), Postives = 38/46 (82.61%), Query Frame = -2 
Query: 1404 GYSIYIN*IQPRTSNKVNKMVSAMPYYAYRLMVRPNEN*QLNYRKI 1541
            GY+I IN ++P TSN++NKMVSAM +YAYRLMVR  EN  LNYR++
Sbjct:  367 GYTININQVKPGTSNQINKMVSAMSFYAYRLMVRSTENHLLNYRQL 412
[Close]
BLAST of SMED30013634 vs. TrEMBL
Match: A0A4Y2CIJ7 (ATP-dependent DNA helicase (Fragment) OS=Araneus ventricosus OX=182803 GN=pif1_43 PE=3 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 4.388e-66
Identity = 93/147 (63.27%), Postives = 113/147 (76.87%), Query Frame = -1 
Query:  550 LTSKCNSPHKLVLKIGSLVMLLRNLDPPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAKDKNWKPKMLDMTKY*NNINYNAI 990
            L   C   H+LVLK+G  +MLLRNLDPP++ NGSRL VKAL+ ++I+ATIL GP+EG+H LI RIPL  TDLPFSFQRLQFP+++A A TINK QGQSL VTGLDLT+E FS+GQLYVGMSRAKD +    + D  K   NI Y+ +
Sbjct:  563 LNPSCIPLHRLVLKVGCPIMLLRNLDPPKLCNGSRLIVKALHAHIIEATILTGPFEGEHVLIPRIPLIPTDLPFSFQRLQFPLRLAFAITINKAQGQSLRVTGLDLTDECFSHGQLYVGMSRAKDTSTLFIIADEQKETKNIVYSQV 709

HSP 2 Score: 95.9005 bits (237), Expect = 4.388e-66
Identity = 48/94 (51.06%), Postives = 66/94 (70.21%), Query Frame = -2 
Query:  969 KLFNKFGNICQNLNDMINCVYPNADANPFDWKWLQERAMLAPLNVIVEEINYYIQEKIQNNFQTF*SIYKCVNKDEATSYPVEVFNTLHQSVIP 1250
            +L N+F  IC  L ++ N VYP+   N  + +WLQERA+LAPLN  V+EIN+ +QEK+    +T+ SI KC+N +EATSYPVE  N+L+ S IP
Sbjct:  476 RLSNEFCKICPTLENLTNQVYPDITLNIQNIQWLQERAILAPLNEKVKEINFTVQEKVPTAARTYYSIDKCLNDEEATSYPVEFLNSLNPSCIP 569
[Close]
BLAST of SMED30013634 vs. Ensembl Medaka
Match: pif1 (PIF1 5'-to-3' DNA helicase [Source:NCBI gene;Acc:101166662])

HSP 1 Score: 52.373 bits (124), Expect = 8.042e-7
Identity = 38/116 (32.76%), Postives = 61/116 (52.59%), Query Frame = -1 
Query:  619 LKIGSLVMLLRNLDPPQ-IRNGSRLTVKALNNY---VIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAK 954
            LK+G+ VML +NLD  + + NG+R  V+A  +    + +   L G  E    L     +F +       R Q P+++A A +I+K QG +L+   + L    F +GQ YV +SRA+
Sbjct:  466 LKVGAQVMLTKNLDVARGLVNGARGVVEAFESEKKGLPRVRFLCGVTE---VLRPERWVFKSASGVHLSRTQLPLKLAWAISIHKSQGMTLDCVEISLAR-VFESGQAYVALSRAR 577
[Close]
BLAST of SMED30013634 vs. Ensembl Medaka
Match: pif1 (PIF1 5'-to-3' DNA helicase [Source:NCBI gene;Acc:101166662])

HSP 1 Score: 52.373 bits (124), Expect = 8.520e-7
Identity = 38/116 (32.76%), Postives = 61/116 (52.59%), Query Frame = -1 
Query:  619 LKIGSLVMLLRNLDPPQ-IRNGSRLTVKALNNY---VIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAK 954
            LK+G+ VML +NLD  + + NG+R  V+A  +    + +   L G  E    L     +F +       R Q P+++A A +I+K QG +L+   + L    F +GQ YV +SRA+
Sbjct:  466 LKVGAQVMLTKNLDVARGLVNGARGVVEAFESEKKGLPRVRFLCGVTE---VLRPERWVFKSASGVHLSRTQLPLKLAWAISIHKSQGMTLDCVEISLAR-VFESGQAYVALSRAR 577
[Close]
BLAST of SMED30013634 vs. Planmine SMEST
Match: SMESG000065933.1 (SMESG000065933.1)

HSP 1 Score: 241.506 bits (615), Expect = 2.359e-78
Identity = 117/119 (98.32%), Postives = 118/119 (99.16%), Query Frame = -1 
Query:  577 MLLRNLDPPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVGMSRAKDKNWKPKMLDMTKY 933
            MLLRNLDPPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLT+EYFSNGQLYVGMSR KDKNWKPKMLDMTKY
Sbjct:    1 MLLRNLDPPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTDEYFSNGQLYVGMSRPKDKNWKPKMLDMTKY 119
[Close]
BLAST of SMED30013634 vs. Planmine SMEST
Match: SMESG000079276.1 (SMESG000079276.1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.734e-23
Identity = 37/75 (49.33%), Postives = 55/75 (73.33%), Query Frame = -1 
Query:  742 HKLVLKIGSLVMLLRNLDPPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPI 966
            H L+LKIG  ++LLRN++ PQ+ NG+RL+VK +   VI+ATILN  ++G+  L+ RIP+ STD+PF F  + F +
Sbjct:  106 HILILKIGVPIILLRNINRPQLCNGTRLSVKIIMKKVIEATILNAKFKGEDVLLSRIPMVSTDIPFEFNCVHFSV 180

HSP 2 Score: 46.595 bits (109), Expect = 1.734e-23
Identity = 30/91 (32.97%), Postives = 45/91 (49.45%), Query Frame = -2 
Query:  969 GNICQ---NLNDMINCVYPNADANPFDWKWLQERAMLAPLNVIVEEINYYIQEKIQNNFQTF*SIYKCVNKDEATSYPVEVFNTLHQSVIP 1232
             NIC+     +++I+ V+PN      +  W   RA+LA +N  V  IN+ IQ  I +   T+  I   +N+DE  +Y  E  N L    IP
Sbjct:   14 ANICKMTSTKDELIHQVFPNITRKYKNHHWFSSRAILASINCGVNAINFKIQSGIPDEATTYKPIDTGMNQDEVVNYLSEFLNLLDLPDIP 104
[Close]
BLAST of SMED30013634 vs. Planmine SMEST
Match: SMESG000003902.1 (SMESG000003902.1)

HSP 1 Score: 95.5153 bits (236), Expect = 5.213e-22
Identity = 49/78 (62.82%), Postives = 59/78 (75.64%), Query Frame = -2 
Query:  966 CVYPNADANPFDWKWLQERAMLAPLNVIVEEINYYIQEKIQNNFQTF*SIYKCVNKDEATSYPVEVFNTLHQS-VIPH 1196
            C+Y NADAN FD KWLQ+R +L+  NV VEEI Y+ QEKIQ N +TF SI KCVN+D+ATSY VE  NTL+ S  +PH
Sbjct:    3 CLYSNADANLFDQKWLQDRVILSTRNVKVEEIYYFKQEKIQTNPRTFLSIGKCVNEDKATSYSVEFLNTLNPSGFLPH 80

HSP 2 Score: 28.1054 bits (61), Expect = 5.213e-22
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = -1 
Query:  925 PHKLVLKIGSLVMLL 969
            PHKL+LK+GS VMLL
Sbjct:   79 PHKLMLKVGSPVMLL 93
[Close]
BLAST of SMED30013634 vs. Planmine SMEST
Match: SMESG000042141.1 (SMESG000042141.1)

HSP 1 Score: 88.9669 bits (219), Expect = 5.171e-21
Identity = 47/99 (47.47%), Postives = 63/99 (63.64%), Query Frame = -1 
Query:  673 PHKLVLKIGSLVMLLRNLDPPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFSTDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDL 969
            P  L+LKIG  ++LLRN++ PQ+ N            VI+ATILNG + G + L+ RIP+  TD+PF F  LQFP++ A   T N  QGQSL+V G+DL
Sbjct:   31 PCILILKIGLPIILLRNINKPQLCNDKN---------VIEATILNGKFNGGNVLLPRIPIVPTDMPFEFNSLQFPVRFAFVMTTNNAQGQSLQVFGIDL 120
[Close]
BLAST of SMED30013634 vs. Planmine SMEST
Match: SMESG000003902.1 (SMESG000003902.1)

HSP 1 Score: 95.5153 bits (236), Expect = 7.188e-21
Identity = 49/78 (62.82%), Postives = 59/78 (75.64%), Query Frame = -2 
Query:  966 CVYPNADANPFDWKWLQERAMLAPLNVIVEEINYYIQEKIQNNFQTF*SIYKCVNKDEATSYPVEVFNTLHQS-VIPH 1196
            C+Y NADAN FD KWLQ+R +L+  NV VEEI Y+ QEKIQ N +TF SI KCVN+D+ATSY VE  NTL+ S  +PH
Sbjct:    3 CLYSNADANLFDQKWLQDRVILSTRNVKVEEIYYFKQEKIQTNPRTFLSIGKCVNEDKATSYSVEFLNTLNPSGFLPH 80
The following BLAST results are available for this feature:
BLAST of SMED30013634 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
PIF18.299e-732.81PIF1 5'-to-3' DNA helicase [Source:HGNC Symbol;Acc... [more]
PIF18.761e-732.81PIF1 5'-to-3' DNA helicase [Source:HGNC Symbol;Acc... [more]
PIF18.761e-732.81PIF1 5'-to-3' DNA helicase [Source:HGNC Symbol;Acc... [more]
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BLAST of SMED30013634 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Y46B2A.22.074e-1839.67ATP-dependent DNA helicase [Source:UniProtKB/TrEM... [more]
F33H12.62.108e-1839.67ATP-dependent DNA helicase [Source:UniProtKB/TrEM... [more]
F59H6.52.127e-1839.67ATP-dependent DNA helicase [Source:UniProtKB/TrEM... [more]
ZK250.92.157e-1839.67ATP-dependent DNA helicase [Source:UniProtKB/TrEM... [more]
Y16E11A.22.157e-1839.67ATP-dependent DNA helicase [Source:UniProtKB/TrEM... [more]
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BLAST of SMED30013634 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Pif18.336e-630.95gene:FBgn0031540 transcript:FBtr0077569[more]
Pif18.336e-630.95gene:FBgn0031540 transcript:FBtr0343239[more]
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BLAST of SMED30013634 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013634 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013634 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013634 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q9H611|PIF1_HUMAN4.207e-632.81ATP-dependent DNA helicase PIF1 OS=Homo sapiens OX... [more]
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BLAST of SMED30013634 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4Y2I5Q62.840e-6763.27ATP-dependent DNA helicase OS=Araneus ventricosus ... [more]
A0A4Y2LYL75.267e-6762.59ATP-dependent DNA helicase (Fragment) OS=Araneus v... [more]
A0A4Y2K3R11.521e-6663.27ATP-dependent DNA helicase OS=Araneus ventricosus ... [more]
A0A4Y2CDA42.525e-6663.27ATP-dependent DNA helicase OS=Araneus ventricosus ... [more]
A0A4Y2CIJ74.388e-6663.27ATP-dependent DNA helicase (Fragment) OS=Araneus v... [more]
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BLAST of SMED30013634 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013634 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013634 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013634 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013634 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pif18.042e-732.76PIF1 5'-to-3' DNA helicase [Source:NCBI gene;Acc:1... [more]
pif18.520e-732.76PIF1 5'-to-3' DNA helicase [Source:NCBI gene;Acc:1... [more]
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BLAST of SMED30013634 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000065933.12.359e-7898.32SMESG000065933.1[more]
SMESG000079276.11.734e-2349.33SMESG000079276.1[more]
SMESG000003902.15.213e-2262.82SMESG000003902.1[more]
SMESG000042141.15.171e-2147.47SMESG000042141.1[more]
SMESG000003902.17.188e-2162.82SMESG000003902.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013634 ID=SMED30013634|Name=SMED30013634|organism=Schmidtea mediterranea sexual|type=transcript|length=1542bp
GGGAATTTTTCGAAACTGAAAAAGGTTTTATGCTATAAATATGAAACAAT
ATGTAATACAATTATGAATTGACTAATATTGTATCCAGTTCATTCACTAA
CATTCGCTAACTAACTCAATCAATAGACGCGTGCAGTTAATCTCGATTAT
AAAACAACTTATATCTGCCATTCATTAAAGTCTTATCATGAATATTTATT
TTAATTCAATAATTCATATGCTACAATTCTTCAAATTACTATATTATATA
TCAATATTATTCTTGATTAATTCACGAATTGTTGAAGAATTATCGAATTA
AAATAAATATTCATGGTTAGACTTTATTGAATGAATAATATTATTCTAAT
CGCGATTAACTGCACGCGTCTATTGAGTGAGTTAGTTAGCGAATGTCAGT
TAATGAGCTGGATACAATATTAGTGAATTGATTATTGTATTACATATTGC
TTCACTTCCGTTTATTCTCGTTCTAAAATATAAATTTTTAGTATTGTTGG
TGCAACCAGTTATCAATTAATTAAAAGTATGTTATATTTAGCAGCGGTGT
ATAGCATTATAGTTGATGTTATTTTAGTACTTGGTCATATCCAACATTTT
GGGTTTCCAATTTTTGTCTTTTGCTCTTGACATACCAACATATAATTGTC
CGTTTGAGAAATATTCATTCGTCAAATCTAAACCAGTAACTTCAAGAGAT
TGCCCTTGGAATTTATTAATAGTGTTTGCTAGTGCAATCTGAATAGGAAA
TTGTAAACGTTGAAATGAAAACGGCAAGTCTGTTGAAAATAATGGAATTC
TTTGTATAAGAGCGTGCTTACCTTCATAAGGTCCGTTTAAAATTGTCGCT
TTAATGACATAGTTATTTAGAGCTTTGACTGTCAATCTTGATCCATTGCG
TATTTGTGGTGGATCTAAATTTCTCAACAACATGACAAGAGATCCGATTT
TTAACACCAATTTGTGTGGGGAATTACACTTTGATGTAAGGTATTAAAAA
CTTCTACGGGGTAGCTTGTTGCTTCGTCTTTATTCACACATTTGTATATT
GATTAAAATGTCTGAAAATTGTTTTGAATCTTTTCTTGTATATAATAGTT
AATTTCTTCAACTATGACATTCAGTGGTGCTAACATTGCACGTTCTTGAA
GCCATTTCCAATCGAAAGGATTTGCATCGGCATTTGGGTACACACAATTA
ATCATATCATTTAAATTTTGACAAATATTTCCGAATTTATTGAAAAGTTT
ATTTCCACACTTTCAGTAGTTTCAAGCTGACTGTTTTCGAGGTAAATATC
AAGTTAATATCGTTCAGTGATAGGAAATCCGAAAATTTGCATGCCACTTC
GTTACTTTATATGTAGCGACCTGCTTAGTACTCAGCTATTTCATCAATTT
CGGGATTTTTCGATAATTTAGCTGTCAATTCTCATTTGGTCGAACCATCA
ATCTATATGCATAATACGGCATAGCCGATACCATTTTATTAACCTTATTT
GACGTCCTTGGCTGAATTTAATTAATGTAGATGGAATAACCG
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protein sequence of SMED30013634-orf-1

>SMED30013634-orf-1 ID=SMED30013634-orf-1|Name=SMED30013634-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=120bp
MLLRNLDPPQIRNGSRLTVKALNNYVIKATILNGPYEGKHALIQRIPLFS
TDLPFSFQRLQFPIQIALANTINKFQGQSLEVTGLDLTNEYFSNGQLYVG
MSRAKDKNWKPKMLDMTKY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR027417P-loop_NTPase
IPR010285DNA_helicase_pif1-like
Vocabulary: molecular function
TermDefinition
GO:0003678DNA helicase activity
Vocabulary: biological process
TermDefinition
GO:0006281DNA repair
GO:0000723telomere maintenance