SMED30013624

Overview
NameSMED30013624
Smed IDSMED30013624
Length (bp)841
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013624

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
reproductive organSMED30013624SMESG000032167.1 SMESG000032101.1 Contig32718newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30013624SMESG000032167.1 SMESG000032101.1 Contig32718uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30013624 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.006274:15653..21076 -4023
v31.006274:34292..41675 -3771
v31.008900:4520..12007 -3168
Homology
[Close]
BLAST of SMED30013624 vs. Planmine SMEST
Match: SMESG000032167.1 (SMESG000032167.1)

HSP 1 Score: 437.958 bits (1125), Expect = 1.149e-147
Identity = 214/261 (81.99%), Postives = 233/261 (89.27%), Query Frame = 2 
Query:    5 NVTSNSILFQWKQQKNL----KYEIIVQPNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
             VT+N I   W+ ++ L    ++E++V P G IY+T ET  T  NL PC+ YC QVR ++ ++ SELS++KCQ+T IAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTN+TNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKE LILVTSKPVKPIEIVVFNVTSTSFQIRWKMP EVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNI+
Sbjct:  367 GVTTNMIEIYWRPEEKLGLADEFEVLVIPIGAIYRTKETDLTFVNLLPCRTYCFQVRIFNFWTSSELSEKKCQSTKIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNRTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKEMLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPPEVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIT 627

HSP 2 Score: 224.557 bits (571), Expect = 6.738e-67
Identity = 121/262 (46.18%), Postives = 162/262 (61.83%), Query Frame = 2 
Query:    5 NVTSNSILFQWKQQKNL----KYEIIV-QPNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
            NVTS S   +WK    +    KYEII+  PN  IY+T +T FTL NL PC  YC Q+  ++ FS+S  SK+ CQNTNI  FDEV+L P+     P +L WTYQNTL CS I F + YY    +  IY+  TN   ++L++I++   YFAKVKPE+DG+FGNFS+E  I++   P  P  I    V S   +I W    E+   + YEI++L P    Y+TKQT+FTL+NLLPC +YCF I +FN    S  S +IC+ TN++
Sbjct:  549 NVTSTSFQIRWKMPPEVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNITLFDEVVLKPIESTQLPAVLYWTYQNTLNCSHIIFYIEYYLENNKKNIYSISTNDTMVKLEQIKYKTKYFAKVKPEFDGLFGNFSEELEIVINKMPETP-SITKIEVASKWIRISWNSFEEMGPEDGYEIVMLFPKTSTYRTKQTNFTLENLLPCRKYCFGIRSFNLVGISNLSMEICEKTNLT 809

HSP 3 Score: 214.927 bits (546), Expect = 1.656e-63
Identity = 113/254 (44.49%), Postives = 161/254 (63.39%), Query Frame = 2 
Query:   56 KYEIIVQ-PNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIA-------------YFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
            +YEII++ PN  IY+T +T FTL NL PC  YC Q+  ++ F +S+ SK+  Q TNI+              FDEV+L P+     P +L WTYQNTL CS+I F + YY    +  IY+  TN   ++L++I++   YFAKVKPE+DG+FGNFS+E  I++   P  P  I V  +++T  +I W+ P+++E  +KYEI+++H  KI Y+T +T F LDNLL C +YCF I  FN   +S  SK+ICQNT I+
Sbjct:   10 EYEIILRHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFGESKKSKKIYQKTNISIKTIQNIIYDNLVLFDEVVLKPIESTQLPAVLYWTYQNTLNCSNIIFYIEYYLENNKKNIYSISTNDTMVKLEQIKYKTKYFAKVKPEFDGLFGNFSEELEIVINKMPDPPSNIKVTALSTTFIEISWESPKDIESTDKYEIVVIHSEKITYQTYKTKFKLDNLLTCRQYCFAIQVFNKLGKSELSKEICQNTKIA 263

HSP 4 Score: 196.052 bits (497), Expect = 1.073e-56
Identity = 110/266 (41.35%), Postives = 165/266 (62.03%), Query Frame = 2 
Query:    2 VNVTSNSILF---QWKQQKNL----KYEIIVQPNGTI-YQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
            + VT+ S  F    W+  K++    KYEI+V  +  I YQT +T+F L NL  C++YC  ++ ++   KSELSKE CQNT IA FDEV L+PV     PV LE +Y NTL CS+I F V YY     + +Y   TN    ++ ++E+++ Y+ K + EY+GMF N+SKE  IL+T  P+ P ++ V  VT+   +I W+   ++   +++E++++ P   IY+TK+T  T  NLLPC  YCFQ+  FNF++ S  S+K CQ+T I+
Sbjct:  181 IKVTALSTTFIEISWESPKDIESTDKYEIVVIHSEKITYQTYKTKFKLDNLLTCRQYCFAIQVFNKLGKSELSKEICQNTKIASFDEVTLNPVNSQKLPVTLEVSYLNTLNCSNILFIVKYYSEIDSHPVYF-ITNETATEIQEMEYNIKYYVKARCEYEGMFSNYSKELEILITKVPITPSQVKVSGVTTNMIEIYWRPEEKLGLADEFEVLVI-PIGAIYRTKETDLTFVNLLPCRTYCFQVRIFNFWTSSELSEKKCQSTKIA 444

HSP 5 Score: 140.198 bits (352), Expect = 3.372e-37
Identity = 81/192 (42.19%), Postives = 113/192 (58.85%), Query Frame = 2 
Query:    2 VNVTSNSILFQWKQQKNLK----YEIIVQ-PNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVK--PIEIVVFNVTSTS 556
            + V S  I   W   + +     YEI++  P  + Y+T +T FTL NL PC++YC  +R+++    S LS E C+ TN+  FDEV+L P+  N FPV+L WTY NT  CS I F V YY    +  IY+  TN+ E +L+KIE +  Y  KV+P+Y+ MFGNFSK  L L   K +K     I  FN+TS++
Sbjct:  730 IEVASKWIRISWNSFEEMGPEDGYEIVMLFPKTSTYRTKQTNFTLENLLPCRKYCFGIRSFNLVGISNLSMEICEKTNLTAFDEVVLKPLQDNSFPVVLSWTYINTSNCSGILFIVEYYSDKEDKDIYSITTNKTESELEKIEFNKKYAVKVRPKYEEMFGNFSK-ILELFIKKDIKWEKKTISTFNITSSA 920

HSP 6 Score: 122.479 bits (306), Expect = 4.629e-31
Identity = 59/67 (88.06%), Postives = 61/67 (91.04%), Query Frame = 2 
Query:  575 MPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
            MP EVE+PN+YEIIL HPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFF ES KSKKI Q TNIS
Sbjct:    1 MPLEVEYPNEYEIILRHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFGESKKSKKIYQKTNIS 67
[Close]
BLAST of SMED30013624 vs. Planmine SMEST
Match: SMESG000032101.1 (SMESG000032101.1)

HSP 1 Score: 261.922 bits (668), Expect = 1.644e-82
Identity = 144/261 (55.17%), Postives = 170/261 (65.13%), Query Frame = 2 
Query:    5 NVTSNSILFQWKQQKNL----KYEIIVQPNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
             VT+N I   W+ ++ L    ++E++V P G IY+T ET  T  NL PC+ YC QVR ++ ++ SELS++KCQ+T I  F +V LS +     P IL+W Y+NT  CS I F V YY             + KE+                            E +  VT+KPVKPIEIVVFNVTSTSFQI WKMP EVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNI+
Sbjct:  111 GVTTNMIEIYWRPEEKLGLADEFEVLVIPIGAIYRTKETDLTFVNLLPCRTYCFQVRIFNFWTSSELSEKKCQSTKIGSFGQVTLSSIQTMTLPFILQWYYENTFNCSGISFLVHYY-------------SEKEI----------------------------ENIFSVTTKPVKPIEIVVFNVTSTSFQILWKMPPEVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIT 330

HSP 2 Score: 217.238 bits (552), Expect = 1.379e-65
Identity = 118/262 (45.04%), Postives = 159/262 (60.69%), Query Frame = 2 
Query:    5 NVTSNSILFQWKQQKNL----KYEIIV-QPNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
            NVTS S    WK    +    KYEII+  PN  IY+T +T FTL NL PC  YC Q+  ++ FS+S  SK+ CQNTNI  FDE +L P+     P +L WTYQNTL CS+I F + YY    +  IY+  TN   ++L++I++   YFA+VKP +DG+FGNFS+E  I++   P  P  I    V S   +I W    E+   + YEI++L P    Y+TKQT+FTL NLLPC +YCF I +FN    S  S +IC+ TN++
Sbjct:  252 NVTSTSFQILWKMPPEVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNITLFDEAVLKPIESTQLPAVLYWTYQNTLNCSNIIFYIEYYLENNKKNIYSISTNDTMVKLEQIKYKTKYFARVKPIFDGLFGNFSEELEIVINKMPETP-SITKIEVASKWIRISWNPFEEMGPEDGYEIVMLFPKTSTYRTKQTNFTLQNLLPCRKYCFGIRSFNLVGISNLSMEICEKTNLT 512

HSP 3 Score: 124.02 bits (310), Expect = 1.102e-31
Identity = 66/160 (41.25%), Postives = 96/160 (60.00%), Query Frame = 2 
Query:    2 VNVTSNSILFQWKQQKNLK----YEIIVQ-PNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMF 466
            + V S  I   W   + +     YEI++  P  + Y+T +T FTL+NL PC++YC  +R+++    S LS E C+ TN+  FDEV+L P+  + FPV+L WTY NT  CS I F V YY    +  IY+  TN+ E +L+KIE +  Y  KV+P+Y+ MF
Sbjct:  433 IEVASKWIRISWNPFEEMGPEDGYEIVMLFPKTSTYRTKQTNFTLQNLLPCRKYCFGIRSFNLVGISNLSMEICEKTNLTAFDEVVLKPLQDSSFPVVLSWTYINTSNCSGILFIVEYYSDKEDKDIYSIITNKTESELEKIEFNKKYAVKVRPKYEEMF 592

HSP 4 Score: 109.383 bits (272), Expect = 2.688e-29
Identity = 51/132 (38.64%), Postives = 85/132 (64.39%), Query Frame = 2 
Query:  380 TNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
            TN    ++ ++E+++ Y+ K + EY+GMF N+SKE  IL+T  P+ P ++ V  VT+   +I W+   ++   +++E++++ P   IY+TK+T  T  NLLPC  YCFQ+  FNF++ S  S+K CQ+T I 
Sbjct:   58 TNETATEIQEMEYNIKYYVKARCEYEGMFSNYSKELEILITKVPITPSQVKVSGVTTNMIEIYWRPEEKLGLADEFEVLVI-PIGAIYRTKETDLTFVNLLPCRTYCFQVRIFNFWTSSELSEKKCQSTKIG 188

HSP 5 Score: 38.1206 bits (87), Expect = 2.688e-29
Identity = 19/44 (43.18%), Postives = 25/44 (56.82%), Query Frame = 1 
Query:  205 MSEYKYCLF**GDIEPCGLQWVSCNLGMDVSEYFRLF*YLLQCR 336
            MS++K C     D+EPC     S +LG  +SEYF LF Y L  +
Sbjct:    1 MSKHKNCFIRRSDLEPCQFTKASSHLGGVLSEYFELFKYSLHSK 44
[Close]
BLAST of SMED30013624 vs. Planmine SMEST
Match: SMESG000032101.1 (SMESG000032101.1)

HSP 1 Score: 261.536 bits (667), Expect = 2.963e-82
Identity = 144/261 (55.17%), Postives = 170/261 (65.13%), Query Frame = 2 
Query:    5 NVTSNSILFQWKQQKNL----KYEIIVQPNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
             VT+N I   W+ ++ L    ++E++V P G IY+T ET  T  NL PC+ YC QVR ++ ++ SELS++KCQ+T I  F +V LS +     P IL+W Y+NT  CS I F V YY             + KE+                            E +  VT+KPVKPIEIVVFNVTSTSFQI WKMP EVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNI+
Sbjct:  111 GVTTNMIEIYWRPEEKLGLADEFEVLVIPIGAIYRTKETDLTFVNLLPCRTYCFQVRIFNFWTSSELSEKKCQSTKIGSFGQVTLSSIQTMTLPFILQWYYENTFNCSGISFLVHYY-------------SEKEI----------------------------ENIFSVTTKPVKPIEIVVFNVTSTSFQILWKMPPEVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIT 330

HSP 2 Score: 216.853 bits (551), Expect = 2.242e-65
Identity = 118/262 (45.04%), Postives = 159/262 (60.69%), Query Frame = 2 
Query:    5 NVTSNSILFQWKQQKNL----KYEIIV-QPNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
            NVTS S    WK    +    KYEII+  PN  IY+T +T FTL NL PC  YC Q+  ++ FS+S  SK+ CQNTNI  FDE +L P+     P +L WTYQNTL CS+I F + YY    +  IY+  TN   ++L++I++   YFA+VKP +DG+FGNFS+E  I++   P  P  I    V S   +I W    E+   + YEI++L P    Y+TKQT+FTL NLLPC +YCF I +FN    S  S +IC+ TN++
Sbjct:  252 NVTSTSFQILWKMPPEVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNITLFDEAVLKPIESTQLPAVLYWTYQNTLNCSNIIFYIEYYLENNKKNIYSISTNDTMVKLEQIKYKTKYFARVKPIFDGLFGNFSEELEIVINKMPETP-SITKIEVASKWIRISWNPFEEMGPEDGYEIVMLFPKTSTYRTKQTNFTLQNLLPCRKYCFGIRSFNLVGISNLSMEICEKTNLT 512

HSP 3 Score: 131.339 bits (329), Expect = 3.385e-34
Identity = 69/163 (42.33%), Postives = 99/163 (60.74%), Query Frame = 2 
Query:    2 VNVTSNSILFQWKQQKNLK----YEIIVQ-PNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNF 475
            + V S  I   W   + +     YEI++  P  + Y+T +T FTL+NL PC++YC  +R+++    S LS E C+ TN+  FDEV+L P+  + FPV+L WTY NT  CS I F V YY    +  IY+  TN+ E +L+KIE +  Y  KV+P+Y+ MFGNF
Sbjct:  433 IEVASKWIRISWNPFEEMGPEDGYEIVMLFPKTSTYRTKQTNFTLQNLLPCRKYCFGIRSFNLVGISNLSMEICEKTNLTAFDEVVLKPLQDSSFPVVLSWTYINTSNCSGILFIVEYYSDKEDKDIYSIITNKTESELEKIEFNKKYAVKVRPKYEEMFGNF 595

HSP 4 Score: 108.997 bits (271), Expect = 2.870e-29
Identity = 51/132 (38.64%), Postives = 85/132 (64.39%), Query Frame = 2 
Query:  380 TNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
            TN    ++ ++E+++ Y+ K + EY+GMF N+SKE  IL+T  P+ P ++ V  VT+   +I W+   ++   +++E++++ P   IY+TK+T  T  NLLPC  YCFQ+  FNF++ S  S+K CQ+T I 
Sbjct:   58 TNETATEIQEMEYNIKYYVKARCEYEGMFSNYSKELEILITKVPITPSQVKVSGVTTNMIEIYWRPEEKLGLADEFEVLVI-PIGAIYRTKETDLTFVNLLPCRTYCFQVRIFNFWTSSELSEKKCQSTKIG 188

HSP 5 Score: 38.1206 bits (87), Expect = 2.870e-29
Identity = 19/44 (43.18%), Postives = 25/44 (56.82%), Query Frame = 1 
Query:  205 MSEYKYCLF**GDIEPCGLQWVSCNLGMDVSEYFRLF*YLLQCR 336
            MS++K C     D+EPC     S +LG  +SEYF LF Y L  +
Sbjct:    1 MSKHKNCFIRRSDLEPCQFTKASSHLGGVLSEYFELFKYSLHSK 44
[Close]
BLAST of SMED30013624 vs. Planmine SMEST
Match: SMESG000032101.1 (SMESG000032101.1)

HSP 1 Score: 217.238 bits (552), Expect = 4.658e-66
Identity = 118/262 (45.04%), Postives = 159/262 (60.69%), Query Frame = 2 
Query:    5 NVTSNSILFQWKQQKNL----KYEIIV-QPNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
            NVTS S    WK    +    KYEII+  PN  IY+T +T FTL NL PC  YC Q+  ++ FS+S  SK+ CQNTNI  FDE +L P+     P +L WTYQNTL CS+I F + YY    +  IY+  TN   ++L++I++   YFA+VKP +DG+FGNFS+E  I++   P  P  I    V S   +I W    E+   + YEI++L P    Y+TKQT+FTL NLLPC +YCF I +FN    S  S +IC+ TN++
Sbjct:  199 NVTSTSFQILWKMPPEVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNITLFDEAVLKPIESTQLPAVLYWTYQNTLNCSNIIFYIEYYLENNKKNIYSISTNDTMVKLEQIKYKTKYFARVKPIFDGLFGNFSEELEIVINKMPETP-SITKIEVASKWIRISWNPFEEMGPEDGYEIVMLFPKTSTYRTKQTNFTLQNLLPCRKYCFGIRSFNLVGISNLSMEICEKTNLT 459

HSP 2 Score: 177.563 bits (449), Expect = 3.646e-51
Identity = 86/90 (95.56%), Postives = 88/90 (97.78%), Query Frame = 2 
Query:  506 KPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
            +PVKPIEIVVFNVTSTSFQI WKMP EVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNI+
Sbjct:  188 EPVKPIEIVVFNVTSTSFQILWKMPPEVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIT 277

HSP 3 Score: 124.02 bits (310), Expect = 8.283e-32
Identity = 66/161 (40.99%), Postives = 96/161 (59.63%), Query Frame = 2 
Query:    2 VNVTSNSILFQWKQQKNLK----YEIIVQ-PNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFG 469
            + V S  I   W   + +     YEI++  P  + Y+T +T FTL+NL PC++YC  +R+++    S LS E C+ TN+  FDEV+L P+  + FPV+L WTY NT  CS I F V YY    +  IY+  TN+ E +L+KIE +  Y  KV+P+Y+ MF 
Sbjct:  380 IEVASKWIRISWNPFEEMGPEDGYEIVMLFPKTSTYRTKQTNFTLQNLLPCRKYCFGIRSFNLVGISNLSMEICEKTNLTAFDEVVLKPLQDSSFPVVLSWTYINTSNCSGILFIVEYYSDKEDKDIYSIITNKTESELEKIEFNKKYAVKVRPKYEEMFD 540
[Close]
BLAST of SMED30013624 vs. Planmine SMEST
Match: SMESG000032056.1 (SMESG000032056.1)

HSP 1 Score: 215.312 bits (547), Expect = 1.238e-63
Identity = 110/198 (55.56%), Postives = 139/198 (70.20%), Query Frame = 2 
Query:    2 VNVTSNSILFQWKQQKNL----KYEIIVQPNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPR 583
            VN TSNSI  QWKQ +N     +YEII+QPNGT Y+ NETQFT+RNLQPC+RYC Q+RAYSDFSKSELSKE C  T IA FD+VI S + Y+  PVIL+WTY+NTL C  I F VVYYP   E  I+  KTN   ++L+K+E++ IYFAKVKP+Y   FG FS+   I+   +P  P+ I V ++ +T  +I W+  R
Sbjct:  762 VNGTSNSIHLQWKQPRNFCLSYQYEIIMQPNGTKYKKNETQFTIRNLQPCRRYCFQIRAYSDFSKSELSKEICHETKIASFDKVIPSSLHYDKLPVILQWTYENTLNCFGISFQVVYYPKNDEKDIHYLKTNETRIKLEKVEYNKIYFAKVKPQYKEQFGEFSEVIEIITIKRPDPPLNIEVLDLFTTFIEISWESRR 959

HSP 2 Score: 203.756 bits (517), Expect = 1.968e-59
Identity = 114/266 (42.86%), Postives = 164/266 (61.65%), Query Frame = 2 
Query:    2 VNVTSNSILF---QWKQQKNL----KYEIIVQPNGTI-YQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
            + VT+ S  F    W+  K++    KYEI+V     I YQT ET+F L NL  C++YC  +R ++   KSELSKE CQNT IA FDEV L+PV     PV LE TY NTL CS+I F V YY     + +Y   TN    ++ ++E+++ Y+ K + EY+GMF N+SKE  IL+T  P+ P ++ V  VT+   +I W    ++   +++E+++L P + IY+TK+T  T+ NLLPC  YCFQ+  FNF++ S  S+K CQ+T I 
Sbjct:  395 IKVTALSTTFIEISWESPKDIESTDKYEIVVIHQEKITYQTYETKFKLDNLLTCRQYCFAIRVFNKLGKSELSKEICQNTKIASFDEVTLNPVKSQKLPVTLEVTYLNTLNCSNILFKVKYYSDMDSDPVYF-ITNETATEIQEMEYNIKYYVKARCEYEGMFSNYSKELEILITKVPITPSQVKVSGVTTNMIEIYWMPEEKLGLADEFEVLVL-PIRAIYRTKETILTIGNLLPCRTYCFQVRIFNFWTSSELSEKKCQSTKIG 658

HSP 3 Score: 201.06 bits (510), Expect = 1.680e-58
Identity = 111/261 (42.53%), Postives = 154/261 (59.00%), Query Frame = 2 
Query:    5 NVTSNSILFQWKQQKNL----KYEIIVQPNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
             VT+N I   W  ++ L    ++E++V P   IY+T ET  T+ NL PC+ YC QVR ++ ++ SELS++KCQ+T I  F +V LS +     P ILEW+Y+NT  C  I F V YY        ++  T + E+ L K+E++  YFAKVKP++    G FSKE  I +T     PIE+ V N TS S  ++WK PR      +YEII + PN   YK  +T FT+ NL PC  YCFQI  ++ FS+S  SK+IC  T I+
Sbjct:  581 GVTTNMIEIYWMPEEKLGLADEFEVLVLPIRAIYRTKETILTIGNLLPCRTYCFQVRIFNFWTSSELSEKKCQSTKIGSFGQVTLSAIQTMTLPFILEWSYENTFNCFGISFLVHYYSEKEIENFFSVTTKKTEILLKKVEYERKYFAKVKPKFGREDGQFSKEIKIFITRDLQAPIELTVVNGTSNSIHLQWKQPRNFCLSYQYEII-MQPNGTKYKKNETQFTIRNLQPCRRYCFQIRAYSDFSKSELSKEICHETKIA 840

HSP 4 Score: 197.593 bits (501), Expect = 2.845e-57
Identity = 105/241 (43.57%), Postives = 155/241 (64.32%), Query Frame = 2 
Query:   59 YEI-IVQPNGTIYQTNETQFTLRNLQPCQRYCIQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQNTLGCSDIFFNVVYYPTYRENI-IYTNKTNRKELQLDKIEHDLIYFAKVKPEYDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNIS 775
            YEI + Q    IY++ +T F L N+  C++YC  +RA++ + KSE+SKEKC N NI    +V++ P+     PVIL WTYQNT  CS I F +V Y + R+N+ ++T KTN+ E+ LDKI+ + IY  +V+P Y    G F+++ +I V   P  P  I V  +++T  +I W+ P+++E  +KYEI+++H  KI Y+T +T F LDNLL C +YCF I  FN   +S  SK+ICQNT I+
Sbjct:  239 YEINVFQATEVIYKSYQTNFNLDNVLLCKKYCFGIRAFNSWGKSEISKEKCGNINILSLGKVLVGPIETKRLPVILRWTYQNTFNCSGIEF-IVEYFSLRDNVNLFTYKTNKTEIILDKIQLNEIYNVRVRPTYGEELGPFTEDMIIWVKV-PDPPSNIKVTALSTTFIEISWESPKDIESTDKYEIVVIHQEKITYQTYETKFKLDNLLTCRQYCFAIRVFNKLGKSELSKEICQNTKIA 477

HSP 5 Score: 76.6406 bits (187), Expect = 2.015e-15
Identity = 38/95 (40.00%), Postives = 55/95 (57.89%), Query Frame = 2 
Query:  488 LILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPNKYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKKICQNTNI 772
            L L+T  P  P EI++   +  SF + W+ P  +     YEI +    ++IYK+ QT+F LDN+L C +YCF I  FN + +S  SK+ C N NI
Sbjct:  200 LPLMTYVPTTPTEIIITQSSQKSFNVSWRKPDFIGLIEGYEINVFQATEVIYKSYQTNFNLDNVLLCKKYCFGIRAFNSWGKSEISKEKCGNINI 294
The following BLAST results are available for this feature:
BLAST of SMED30013624 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013624 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013624 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013624 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013624 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013624 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013624 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013624 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013624 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013624 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013624 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013624 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013624 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013624 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000032167.11.149e-14781.99SMESG000032167.1[more]
SMESG000032101.11.644e-8255.17SMESG000032101.1[more]
SMESG000032101.12.963e-8255.17SMESG000032101.1[more]
SMESG000032101.14.658e-6645.04SMESG000032101.1[more]
SMESG000032056.11.238e-6355.56SMESG000032056.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013624 ID=SMED30013624|Name=SMED30013624|organism=Schmidtea mediterranea sexual|type=transcript|length=841bp
GGTCAATGTAACATCAAACTCAATCCTTTTTCAATGGAAGCAGCAGAAAA
ATTTGAAATATGAAATAATTGTACAGCCAAATGGAACTATTTATCAAACA
AACGAAACACAATTTACATTACGAAATTTGCAACCGTGTCAAAGGTATTG
CATTCAAGTCCGTGCCTATAGCGACTTTAGTAAATCAGAATTGTCAAAGG
AAAAATGTCAGAATACAAATATTGCCTATTTTGATGAGGTGATATTGAGC
CCTGTGGTTTACAATGGGTTTCCTGTAATCTTGGAATGGACGTATCAGAA
TACTTTAGGTTGTTCTGATATCTTCTTCAATGTCGTATATTACCCAACAT
ACAGAGAAAACATTATTTATACAAATAAAACAAACAGAAAAGAATTGCAA
CTTGATAAAATTGAACACGACCTGATTTACTTTGCAAAGGTCAAACCGGA
ATACGACGGGATGTTTGGTAACTTTTCTAAGGAGACGCTAATACTCGTTA
CATCAAAGCCAGTAAAACCGATCGAAATTGTGGTATTTAATGTGACCTCT
ACGTCATTTCAAATCCGTTGGAAAATGCCCCGTGAAGTTGAATTTCCCAA
CAAATATGAAATTATTTTACTCCATCCAAACAAGATTATATATAAAACCA
AACAAACACATTTCACCTTGGATAATTTGCTACCGTGTACAGAATATTGC
TTCCAAATTAGTACTTTTAATTTCTTCAGCGAATCAACAAAATCAAAGAA
AATATGTCAAAATACAAATATTAGTAAAAATTGTCTAAAGTATTCTAAAA
ATAATTTAATGTTTAGCTTTATTTGATGAGGTCGTCCTGAA
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protein sequence of SMED30013624-orf-1

>SMED30013624-orf-1 ID=SMED30013624-orf-1|Name=SMED30013624-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=275bp
VNVTSNSILFQWKQQKNLKYEIIVQPNGTIYQTNETQFTLRNLQPCQRYC
IQVRAYSDFSKSELSKEKCQNTNIAYFDEVILSPVVYNGFPVILEWTYQN
TLGCSDIFFNVVYYPTYRENIIYTNKTNRKELQLDKIEHDLIYFAKVKPE
YDGMFGNFSKETLILVTSKPVKPIEIVVFNVTSTSFQIRWKMPREVEFPN
KYEIILLHPNKIIYKTKQTHFTLDNLLPCTEYCFQISTFNFFSESTKSKK
ICQNTNISKNCLKYSKNNLMFSFI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR036116FN3_sf
IPR003961FN3_dom
IPR013783Ig-like_fold
IPR015373Interferon/interleukin_rcp_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003961Fibronectin type IIISMARTSM00060FN3_2coord: 170..245
e-value: 2.2E-8
score: 43.9
coord: 1..62
e-value: 54.0
score: 5.2
IPR003961Fibronectin type IIIPROSITEPS50853FN3coord: 172..258
score: 12.805
IPR003961Fibronectin type IIIPROSITEPS50853FN3coord: 1..75
score: 11.18
IPR003961Fibronectin type IIICDDcd00063FN3coord: 2..65
e-value: 2.65775E-6
score: 42.8687
IPR003961Fibronectin type IIICDDcd00063FN3coord: 170..251
e-value: 3.42559E-8
score: 48.2615
IPR015373Interferon/interleukin receptor domainPFAMPF09294Interfer-bindcoord: 15..72
e-value: 1.3E-5
score: 25.5
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 163..257
e-value: 2.0E-10
score: 42.6
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 1..73
e-value: 1.1E-8
score: 37.5
NoneNo IPR availablePANTHERPTHR23036CYTOKINE RECEPTORcoord: 2..88
IPR036116Fibronectin type III superfamilySUPERFAMILYSSF49265Fibronectin type IIIcoord: 2..72
IPR036116Fibronectin type III superfamilySUPERFAMILYSSF49265Fibronectin type IIIcoord: 91..252