Protein FAM221B

Overview


Name	Protein FAM221B
Smed ID	SMED30013590
Length (bp)	1042

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Protein FAM221B (SMED30013590) t-SNE clustered cells

Violin plots show distribution of expression levels for Protein FAM221B (SMED30013590) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Protein FAM221B (SMED30013590) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Protein FAM221B (SMED30013590) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30013590

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 10

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30013590	SMESG000035378.1	SmedASXL_005793	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pharynx	SMED30013590	SMESG000035378.1	dd_Smed_v4_10703_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30013590	SMESG000035378.1	dd_Smed_v4_10703_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30013590	SMESG000035378.1	dd_Smed_v4_10703_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30013590	SMESG000035378.1	dd_Smed_v4_10703_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
head region	SMED30013590	SMESG000035378.1	dd_Smed_v6_10703_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
reproductive organ	SMED30013590	SMESG000035378.1	Contig53091	newmark_ests	PMID:28434803 Rouhana et al., 2017
reproductive organ	SMED30013590	SMESG000035378.1	Contig53091	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
epidermis	SMED30013590	SMESG000035378.1	dd_Smed_v4_10703_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
ciliated epithelial cell	SMED30013590	SMESG000035378.1	dd_Smed_v4_10703_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of Protein FAM221B vs. Ensembl Human
Match: FAM221B (family with sequence similarity 221 member B [Source:HGNC Symbol;Acc:HGNC:30762])

HSP 1 Score: 152.14 bits (383), Expect = 4.931e-42
Identity = 82/196 (41.84%), Postives = 120/196 (61.22%), Query Frame = 3

Query:  222 KKIKPATKYDVINVARAMN-DDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKD 806
            + + PA + +++ VA+AM+ ++F  ++  LF  E++A + A+Q+GLYI +R    P    DC RI  + +CFC HLL+EH   +  +  + C    C C  F ++PSRPEE GEFWL++R  F    ++A+CRCKHSH +H A   PH C+  GC C  F S F+CAACD  W  H+T  +T+  R+  GRP G D
Sbjct:  204 RPVFPARQTELVEVAKAMHREEFGAQVNNLFQWEKDAALNAIQTGLYIGWR---CPHYLWDCFRIGDESRCFCGHLLREHRIIS--DISVPCKVSQCRCFMFCFIPSRPEEVGEFWLKRRATFDPKAWRAQCRCKHSHEEHAA-TGPHPCRHHGCCCGCFESNFLCAACDRRWEEHETFFDTQKTRQRGGRPRGTD 393

[Close]

BLAST of Protein FAM221B vs. Ensembl Human
Match: FAM221A (family with sequence similarity 221 member A [Source:HGNC Symbol;Acc:HGNC:27977])

HSP 1 Score: 65.855 bits (159), Expect = 1.372e-11
Identity = 56/201 (27.86%), Postives = 87/201 (43.28%), Query Frame = 3

Query:  342 VQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEH----DKFTGHEPI-LKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGENNPDIQSLQDALPIN--LQTPIN 923
            +Q+ L++ +R   SP    DC  +  +  CFC+H  K+H    +      PI L C    C C+A+ +VP                 +      +CRCKH   D  +  P   C      CS F+S F CA C    + H TV+ET+ ER    +P+G+D +P+A +  L   +   E    +      +P    L++PI 
Sbjct:   50 LQNRLFVSWR---SPTGM-DCKLVGPETLCFCTHRYKQHKTDLEAIPQQCPIDLPCQVTGCQCRAYLYVP-----------------LNGSQPIRCRCKH-FADQHSAAPGFTCNTCS-KCSGFHSCFTCA-CGQPAYAHDTVVETKQERLAQEKPVGQD-IPYAAMGGLTGFSSLAEGYMRLDDSGIGVPSVEFLESPIT 225

[Close]

BLAST of Protein FAM221B vs. Ensembl Human
Match: FAM221A (family with sequence similarity 221 member A [Source:HGNC Symbol;Acc:HGNC:27977])

HSP 1 Score: 65.4698 bits (158), Expect = 1.569e-11
Identity = 59/216 (27.31%), Postives = 92/216 (42.59%), Query Frame = 3

Query:  249 DVINVARAMNDDFAPKLKELFNPEQ------EACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEH----DKFTGHEPI-LKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGEN 863
            + +   R + +D   KL   F PE+      +     +Q+ L++ +R   SP    DC  +  +  CFC+H  K+H    +      PI L C    C C+A+ +VP                 +      +CRCKH   D  +  P   C      CS F+S F CA C    + H TV+ET+ ER    +P+G+D +P+A +  L   +   E 
Sbjct:   16 EYLEYRRIVGEDDGGKL---FTPEEYEEYKRKVLPLRLQNRLFVSWR---SPTGM-DCKLVGPETLCFCTHRYKQHKTDLEAIPQQCPIDLPCQVTGCQCRAYLYVP-----------------LNGSQPIRCRCKH-FADQHSAAPGFTCNTCS-KCSGFHSCFTCA-CGQPAYAHDTVVETKQERLAQEKPVGQD-IPYAAMGGLTGFSSLAEG 203

[Close]

BLAST of Protein FAM221B vs. Ensembl Human
Match: FAM221B (family with sequence similarity 221 member B [Source:HGNC Symbol;Acc:HGNC:30762])

HSP 1 Score: 65.855 bits (159), Expect = 1.586e-11
Identity = 31/80 (38.75%), Postives = 52/80 (65.00%), Query Frame = 3

Query:  222 KKIKPATKYDVINVARAMN-DDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEH 458
            + + PA + +++ VA+AM+ ++F  ++  LF  E++A + A+Q+GLYI +R    P    DC RI  + +CFC HLL+EH
Sbjct:  204 RPVFPARQTELVEVAKAMHREEFGAQVNNLFQWEKDAALNAIQTGLYIGWR---CPHYLWDCFRIGDESRCFCGHLLREH 280

[Close]

BLAST of Protein FAM221B vs. Ensembl Human
Match: AL133410.3 (Novel protein)

HSP 1 Score: 52.373 bits (124), Expect = 1.478e-8
Identity = 22/49 (44.90%), Postives = 28/49 (57.14%), Query Frame = 3

Query:  660 HKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKD 806
            H   + GC C  F S F+CAACD  W  H+T  +T+  R+  GRP G D
Sbjct:   16 HIGALQGCCCGCFESNFLCAACDRRWEEHETFFDTQKTRQRGGRPRGTD 64

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BLAST of Protein FAM221B vs. Ensembl Zebrafish
Match: fam221a (family with sequence similarity 221 member A [Source:ZFIN;Acc:ZDB-GENE-050320-27])

HSP 1 Score: 59.6918 bits (143), Expect = 5.011e-10
Identity = 57/232 (24.57%), Postives = 93/232 (40.09%), Query Frame = 3

Query:  195 KTHFGDVKIKKIKPATKYDVI----NVARAMNDDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEH----DKFTGHEPI-LKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGEN 863
            K H   +    ++   +Y  I    +  R  ++D   K KE   P +      +Q+ LY+ +        Q DC  I  +  CFC+H  K+H    ++     P+ L C    C C ++ +V                  +      +CRCKH+  +HK     H CK     C+ + S F C  C    + H T++ET  ER   G P+G+  +P+A +  L   +   E 
Sbjct:    3 KIHIDKLAANSVEAYLEYKRIVGEDDGGRLFSEDEYKKYKETVVPGR------IQNRLYVSF----GVPGQIDCKVIGPETTCFCTHRYKQHRTDYEELPKERPVLLPCRVKGCQCASYEYV-----------------HINGSKPVRCRCKHTTSEHK-EDARHLCKKC-THCTGYRSPFPC-GCGEPGYTHVTLIETREERIARGHPVGR-AVPYAAMGGLTGFSSLAEG 203

[Close]

Query:  195 KTHFGDVKIKKIKPATKYDVI----NVARAMNDDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEH----DKFTGHEPI-LKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGEN 863
            K H   +    ++   +Y  I    +  R  ++D   K KE   P +      +Q+ LY+ +        Q DC  I  +  CFC+H  K+H    ++     P+ L C    C C ++ +V                  +      +CRCKH+  +HK     H CK     C+ + S F C  C    + H T++ET  ER   G P+G+  +P+A +  L   +   E 
Sbjct:    3 KIHIDKLAANSVEAYLEYKRIVGEDDGGRLFSEDEYKKYKETVVPGR------IQNRLYVSF----GVPGQIDCKVIGPETTCFCTHRYKQHRTDYEELPKERPVLLPCRVKGCQCASYEYV-----------------HINGSKPVRCRCKHTTSEHK-EDARHLCKKC-THCTGYRSPFPC-GCGEPGYTHVTLIETREERIARGHPVGR-AVPYAAMGGLTGFSSLAEG 203

[Close]

Query:  195 KTHFGDVKIKKIKPATKYDVI----NVARAMNDDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEH----DKFTGHEPI-LKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGEN 863
            K H   +    ++   +Y  I    +  R  ++D   K KE   P +      +Q+ LY+ +        Q DC  I  +  CFC+H  K+H    ++     P+ L C    C C ++ +V                  +      +CRCKH+  +HK     H CK     C+ + S F C  C    + H T++ET  ER   G P+G+  +P+A +  L   +   E 
Sbjct:    3 KIHIDKLAANSVEAYLEYKRIVGEDDGGRLFSEDEYKKYKETVVPGR------IQNRLYVSF----GVPGQIDCKVIGPETTCFCTHRYKQHRTDYEELPKERPVLLPCRVKGCQCASYEYV-----------------HINGSKPVRCRCKHTTSEHK-EDARHLCKKC-THCTGYRSPFPC-GCGEPGYTHVTLIETREERIARGHPVGR-AVPYAAMGGLTGFSSLAEG 203

[Close]

BLAST of Protein FAM221B vs. Ensembl Xenopus
Match: rprd1b (regulation of nuclear pre-mRNA domain containing 1B [Source:Xenbase;Acc:XB-GENE-949983])

HSP 1 Score: 69.707 bits (169), Expect = 3.576e-13
Identity = 55/182 (30.22%), Postives = 81/182 (44.51%), Query Frame = 3

Query:  342 VQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEP-----ILKCLQMNCPCKAFAWVP---SRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGEN 863
            +Q+ LY+ +R   SP    DC  +  +  CFC+H  K+H       P     +L C    C CK+F ++P   SRP                     +CRCKH   +H      H  + + C  S F+S+F C  C    + H TV+ET+ ER   G+P+G D +PFA +  L   +   E 
Sbjct:    3 LQNRLYVSWR---SPTGM-DCKLVGPETPCFCTHRYKQHKTDFQELPKERPLLLPCRVSKCACKSFHYIPLNGSRP--------------------IRCRCKHFADEHSVAGTYHCTQCTKC--SGFHSSFTCG-CSQPAYTHDTVVETKEERLAQGKPVGHD-VPFAAMGGLTGFSSLAEG 156

[Close]

BLAST of Protein FAM221B vs. Ensembl Mouse
Match: Fam221b (family with sequence similarity 221, member B [Source:MGI Symbol;Acc:MGI:2441678])

HSP 1 Score: 157.147 bits (396), Expect = 2.252e-43
Identity = 84/224 (37.50%), Postives = 126/224 (56.25%), Query Frame = 3

Query:  153 IPIDKNGNLIEGQYKTHFGDVKIKKIKPATKYDVINVARAMN-DDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIG----KDW 809
            IP  K G   + + +   G V  + + PA + +++ +A+A++ + F  ++  LF  E+++ ++A+Q+G+YI +R    P    DC RI  + KCFC HLL+EH   +     + C    C C  F ++PSRPEE GEFWL+KR  F    ++A+CRCKH+H +H A    H C+  GC C  F S F+C ACD  W  H+T  ET   R+   RP G    K+W
Sbjct:  266 IPAAKPGLRAKKKKEKRAGGVTSRPVVPAKRAELVEMAKAVHREQFDDQVNNLFQWEKDSTLKAIQTGIYIGWR---CPHYLWDCFRIGDESKCFCGHLLREHQIISDLS--VPCSVSQCRCLMFCFIPSRPEEVGEFWLKKRATFDPKAWRAQCRCKHTHEEHAAT-GAHPCRHRGCFCPSFESNFLCVACDRRWEEHETFFETGDTRRKGKRPYGANNIKNW 483

[Close]

BLAST of Protein FAM221B vs. Ensembl Mouse
Match: Fam221a (family with sequence similarity 221, member A [Source:MGI Symbol;Acc:MGI:2442161])

HSP 1 Score: 65.855 bits (159), Expect = 1.120e-11
Identity = 60/217 (27.65%), Postives = 92/217 (42.40%), Query Frame = 3

Query:  249 DVINVARAMNDDFAPKLKELFNPEQ------EACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEH----DKFTGHEPI-LKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCG-CSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGEN 863
            + +   R + +D   KL   F PE+      +     +Q+ L++ +R   SP    DC  +  +  CFC+H  K+H    +      PI L C    C CKA+ +VP                 +      +CRCKH    H A         + C  CS F+S + CA C    + H TV+ET  ER   G+P+G+D +P+A +  L   +   E 
Sbjct:   16 EYLEYRRIVGEDDGGKL---FTPEEYEEYKKKVLPMRLQNRLFVSWR---SPTGM-DCKLVGPETLCFCTHRYKQHKTDFETIPQQRPIALPCRVSGCRCKAYHYVP-----------------LNGTQPIRCRCKHFADQHSAA---LGFTCNACSKCSGFHSCYTCA-CGQPAYAHDTVVETRQERLAQGKPVGRD-VPYAAMGGLTGFSSLAEG 203

[Close]

BLAST of Protein FAM221B vs. Ensembl Mouse
Match: Fam221a (family with sequence similarity 221, member A [Source:MGI Symbol;Acc:MGI:2442161])

HSP 1 Score: 64.6994 bits (156), Expect = 1.623e-11
Identity = 60/217 (27.65%), Postives = 92/217 (42.40%), Query Frame = 3

Query:  249 DVINVARAMNDDFAPKLKELFNPEQ------EACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEH----DKFTGHEPI-LKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCG-CSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGEN 863
            + +   R + +D   KL   F PE+      +     +Q+ L++ +R   SP    DC  +  +  CFC+H  K+H    +      PI L C    C CKA+ +VP                 +      +CRCKH    H A         + C  CS F+S + CA C    + H TV+ET  ER   G+P+G+D +P+A +  L   +   E 
Sbjct:   16 EYLEYRRIVGEDDGGKL---FTPEEYEEYKKKVLPMRLQNRLFVSWR---SPTGM-DCKLVGPETLCFCTHRYKQHKTDFETIPQQRPIALPCRVSGCRCKAYHYVP-----------------LNGTQPIRCRCKHFADQHSA---ALGFTCNACSKCSGFHSCYTCA-CGQPAYAHDTVVETRQERLAQGKPVGRD-VPYAAMGGLTGFSSLAEG 203

[Close]

BLAST of Protein FAM221B vs. UniProt/SwissProt
Match: sp|Q8C627|F221B_MOUSE (Protein FAM221B OS=Mus musculus OX=10090 GN=Fam221b PE=2 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 1.575e-42
Identity = 84/224 (37.50%), Postives = 126/224 (56.25%), Query Frame = 3

Query:  153 IPIDKNGNLIEGQYKTHFGDVKIKKIKPATKYDVINVARAMN-DDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIG----KDW 809
            IP  K G   + + +   G V  + + PA + +++ +A+A++ + F  ++  LF  E+++ ++A+Q+G+YI +R    P    DC RI  + KCFC HLL+EH   +     + C    C C  F ++PSRPEE GEFWL+KR  F    ++A+CRCKH+H +H A    H C+  GC C  F S F+C ACD  W  H+T  ET   R+   RP G    K+W
Sbjct:  266 IPAAKPGLRAKKKKEKRAGGVTSRPVVPAKRAELVEMAKAVHREQFDDQVNNLFQWEKDSTLKAIQTGIYIGWR---CPHYLWDCFRIGDESKCFCGHLLREHQIISDLS--VPCSVSQCRCLMFCFIPSRPEEVGEFWLKKRATFDPKAWRAQCRCKHTHEEHAAT-GAHPCRHRGCFCPSFESNFLCVACDRRWEEHETFFETGDTRRKGKRPYGANNIKNW 483

[Close]

BLAST of Protein FAM221B vs. UniProt/SwissProt
Match: sp|A6H8Z2|F221B_HUMAN (Protein FAM221B OS=Homo sapiens OX=9606 GN=FAM221B PE=1 SV=2)

HSP 1 Score: 152.14 bits (383), Expect = 2.368e-41
Identity = 82/196 (41.84%), Postives = 120/196 (61.22%), Query Frame = 3

Query:  222 KKIKPATKYDVINVARAMN-DDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKD 806
            + + PA + +++ VA+AM+ ++F  ++  LF  E++A + A+Q+GLYI +R    P    DC RI  + +CFC HLL+EH   +  +  + C    C C  F ++PSRPEE GEFWL++R  F    ++A+CRCKHSH +H A   PH C+  GC C  F S F+CAACD  W  H+T  +T+  R+  GRP G D
Sbjct:  204 RPVFPARQTELVEVAKAMHREEFGAQVNNLFQWEKDAALNAIQTGLYIGWR---CPHYLWDCFRIGDESRCFCGHLLREHRIIS--DISVPCKVSQCRCFMFCFIPSRPEEVGEFWLKRRATFDPKAWRAQCRCKHSHEEHAA-TGPHPCRHHGCCCGCFESNFLCAACDRRWEEHETFFDTQKTRQRGGRPRGTD 393

[Close]

BLAST of Protein FAM221B vs. UniProt/SwissProt
Match: sp|Q66HD8|F221B_RAT (Protein FAM221B OS=Rattus norvegicus OX=10116 GN=Fam221b PE=1 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 5.963e-37
Identity = 79/196 (40.31%), Postives = 117/196 (59.69%), Query Frame = 3

Query:  222 KKIKPATKYDVINVARAMN-DDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKD 806
            + + PA + +++ +A+AM+   F  ++ +LF  E+ + ++A+Q+G+YI +R    P    DC RI  + KCFC HLLKEH   +     + C    C C  F ++PSRPEE GEFWL+KR  F    ++A+CRCKH+H +H A    H C+  GC C+ F S F+CAACD  W  H+T  ETE  ++   RP G +
Sbjct:  309 RPVVPAKRAELVEMAKAMHRKQFDDQVNDLFQWEKNSSLKAIQTGIYIGWR---CPHYLWDCFRIGDESKCFCGHLLKEHQIISDLS--VPCSVSQCRCLMFCFIPSRPEEVGEFWLKKRATFDPKAWRAQCRCKHTHEEHAAT-GSHPCRHRGCYCNSFESNFLCAACDRRWEEHETFFETEETKRRGKRPYGTN 498

[Close]

BLAST of Protein FAM221B vs. UniProt/SwissProt
Match: sp|Q2VPL9|F221A_XENLA (Protein FAM221A OS=Xenopus laevis OX=8355 GN=fam221a PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.837e-13
Identity = 65/213 (30.52%), Postives = 94/213 (44.13%), Query Frame = 3

Query:  267 RAMNDDFAPKLKELFNPEQ-EACMEAV-----QSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEP-----ILKCLQMNCPCKAFAWVP---SRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGEN 863
            R + DD   KL   F PE+ E   + V     Q+ LY+ +R   SP    DC  +  +  CFC+H  K+H       P     +L C    CPCK+F ++P   SRP                     +CRCKH   +H      H  K + C  S F+S+F C  C    + H T++ET+ ER   G+P+G D +P+A +  L   +   E 
Sbjct:   21 RIVGDDDGGKL---FTPEEYEQYKKTVLPMRLQNRLYVSWR---SPCGM-DCKLVGPETACFCTHRYKQHKTDFKELPKERPVLLPCKVSKCPCKSFHYIPLNGSRP--------------------IRCRCKHFADEHSLAGTYHCTKCTKC--SGFHSSFTCG-CGQPAYAHDTIVETKEERLAQGKPVGHD-VPYASMGGLTGFSSLAEG 202

[Close]

BLAST of Protein FAM221B vs. UniProt/SwissProt
Match: sp|Q28J59|F221A_XENTR (Protein FAM221A OS=Xenopus tropicalis OX=8364 GN=fam221a PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 5.061e-13
Identity = 65/213 (30.52%), Postives = 93/213 (43.66%), Query Frame = 3

Query:  267 RAMNDDFAPKLKELFNPEQ-EACMEAV-----QSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEP-----ILKCLQMNCPCKAFAWVP---SRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGEN 863
            R + DD   KL   F PE+ E   + V     Q+ LY+ +R   SP    DC  +  +  CFC+H  K+H       P     +L C    C CK+F ++P   SRP                     +CRCKH   +H      H  + + C  S F+S+F C  C    + H TV+ET+ ER   G+P+G D +PFA +  L   +   E 
Sbjct:   21 RIVGDDDGGKL---FTPEEYEEYKKTVLPMRLQNRLYVSWR---SPTGM-DCKLVGPETPCFCTHRYKQHKTDFQELPKERPLLLPCRVSKCACKSFHYIPLNGSRP--------------------IRCRCKHFADEHSVAGTYHCTQCTKC--SGFHSSFTCG-CSQPAYTHDTVVETKEERLAQGKPVGHD-VPFAAMGGLTGFSSLAEG 202

[Close]

BLAST of Protein FAM221B vs. TrEMBL
Match: A0A4E0RV93 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_007500 PE=4 SV=1)

HSP 1 Score: 341.658 bits (875), Expect = 4.809e-114
Identity = 157/268 (58.58%), Postives = 202/268 (75.37%), Query Frame = 3

Query:   99 KSNIPKSKVVANKASKNPIPIDKNGNLIEGQYKTHFG-DVKIKKIKPATKYDVINVARAMNDDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGENNPDIQSLQDALP 899
            K+N   SKV++  + K   P DK+GN+I G Y+T  G +V+IKKI+PA  YDVI++ARAMNDDFA K KELF+PE EA  EA+QSG+Y+ +RP + P +QQDC RIS+K +CFC HLL +H+KF      LKC Q +C C+A+ +VPSRPEE GE+WL KRRDF  A Y+ KCRCKH+H +H AHPPPH C+  GCGCS F SAF CAACD  W  H+TV ETESER+ AGR +G++W+PF+ELP+L  +A TG +NP+I++L DALP
Sbjct:    2 KNNTESSKVISKISDKTVFPTDKDGNIIPGTYQTQVGVEVRIKKIEPAKHYDVISLARAMNDDFAAKTKELFSPEVEAVREAIQSGVYVSWRPSEKPWDQQDCQRISSKARCFCGHLLNQHEKFNPKHIFLKCEQPSCLCQAYKFVPSRPEEVGEYWLTKRRDFDPAAYRVKCRCKHTHEEHVAHPPPHHCQAKGCGCSGFTSAFACAACDKPWQLHETVFETESERRAAGRQVGEEWMPFSELPELAKIAATGVDNPNIETLADALP 269

[Close]

BLAST of Protein FAM221B vs. TrEMBL
Match: A0A504YHC6 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_01255 PE=4 SV=1)

HSP 1 Score: 342.813 bits (878), Expect = 7.435e-114
Identity = 157/268 (58.58%), Postives = 202/268 (75.37%), Query Frame = 3

Query:   99 KSNIPKSKVVANKASKNPIPIDKNGNLIEGQYKTHFG-DVKIKKIKPATKYDVINVARAMNDDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGENNPDIQSLQDALP 899
            K+N   SKV++  + K   P DK+GN+I G Y+T  G +V+IKKI+PA  YDVI++ARAMNDDFA K KELF+PE EA  EA+QSG+Y+ +RP + P +QQDC RIS+K +CFC HLL +H+KF      LKC Q +C C+A+ +VPSRPEE GE+WL KRRDF  A Y+ KCRCKH+H +H AHPPPH C+  GCGCS F SAF CAACD  W  H+TV ETESER+ AGR +G++W+PF+ELP+L  +A TG +NP+I++L DALP
Sbjct:   40 KNNTESSKVISKISDKTVFPTDKDGNIIPGTYQTQVGVEVRIKKIEPAKHYDVISLARAMNDDFAAKTKELFSPEVEAVREAIQSGVYVSWRPSEKPWDQQDCQRISSKARCFCGHLLNQHEKFNPKHIFLKCKQPSCLCQAYKFVPSRPEEVGEYWLTKRRDFDPAAYRVKCRCKHTHEEHVAHPPPHHCQAKGCGCSGFTSAFACAACDKPWQLHETVFETESERRAAGRQVGEEWMPFSELPELAKIAATGVDNPNIETLADALP 307

[Close]

BLAST of Protein FAM221B vs. TrEMBL
Match: A0A5J4P039 (Uncharacterized protein (Fragment) OS=Paragonimus westermani OX=34504 GN=DEA37_0014402 PE=4 SV=1)

HSP 1 Score: 335.109 bits (858), Expect = 3.056e-111
Identity = 151/249 (60.64%), Postives = 192/249 (77.11%), Query Frame = 3

Query:  153 IPIDKNGNLIEGQYKTHFGDVKIKKIKPATKYDVINVARAMNDDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGENNPDIQSLQDALP 899
             P DKNGN+I G YKT  G+V+IKKI+PA  YDVI++ARAMNDDFA K K+LF+PE +A  EA+ SG+YI +RP + P +QQDC R+S+  +CFC HLL +H  F G    LKC Q  C C+AF +VPSRPEE GEFWLRKRRDF  A Y+ KC+CKH+H +H A+P P++CK  GCGCS F SAFVCAACD  W++HQTV ETE+ER+ AGR +G+ W+PF+ELP L ++A+TG +NPDI++L DALP
Sbjct:   13 FPTDKNGNIIPGTYKTPAGEVRIKKIEPAKHYDVISLARAMNDDFAAKTKKLFSPEVDAVREAIGSGVYIAWRPTEKPWDQQDCQRLSSNARCFCGHLLSQHQNFDGKRAFLKCKQNGCVCQAFNFVPSRPEEVGEFWLRKRRDFDEAVYRVKCKCKHTHEEHAAYPAPYRCKTPGCGCSGFTSAFVCAACDKPWNQHQTVTETEAERRTAGRQVGEAWMPFSELPGLSNIALTGVDNPDIETLADALP 261

[Close]

BLAST of Protein FAM221B vs. TrEMBL
Match: A0A4Z2DB22 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_002703 PE=4 SV=1)

HSP 1 Score: 325.865 bits (834), Expect = 1.512e-107
Identity = 151/285 (52.98%), Postives = 200/285 (70.18%), Query Frame = 3

Query:   87 IMNKKSNIPKSKVVANKASKNPIPIDKNGNLIEGQYKTHFGDVKIKKIKPATKYDVINVARAMNDDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGENNPDIQSLQDALPINLQTPINKSNFRP 941
            + NK   I ++K    K  K P  IDK+ N+I G YKT  G+V+IKKI+   +YDVI++AR++N++FAP+ KELF PE EA  EA+++G Y+ +RP+D P NQQDC R+    +CFC HLL EHD F+    + KC    C CK F ++PSRPEE GEFW+ KR DF  + Y+ KC+CKH+H +H ++P P++CKV GC CS F SAF+CAACD HWH H+TV ETE ERK  GRP+G+ WLPFAELP+L  +A+TG +NPDIQ+L DALP N +  I +    P
Sbjct:    8 VQNKSLRIKQTK---RKEDKQPFLIDKDYNIIPGTYKTPVGEVRIKKIEAPRRYDVISLARSINENFAPRTKELFTPEIEAVKEAMKTGNYVAWRPIDKPWNQQDCQRVCCNSRCFCGHLLNEHDSFSAKIIVPKCNHTGCLCKGFKFIPSRPEEVGEFWITKRSDFDRSAYRVKCKCKHTHEEHASYPVPYQCKVKGCRCSGFSSAFLCAACDKHWHEHETVFETEVERKADGRPVGEAWLPFAELPELAKIALTGVDNPDIQTLMDALPTNSRQNIPEQQILP 289

[Close]

BLAST of Protein FAM221B vs. TrEMBL
Match: A0A0X3P7W5 (Protein FAM221B OS=Schistocephalus solidus OX=70667 GN=F221B PE=4 SV=1)

HSP 1 Score: 322.783 bits (826), Expect = 1.713e-106
Identity = 147/263 (55.89%), Postives = 191/263 (72.62%), Query Frame = 3

Query:  153 IPIDKNGNLIEGQYKTHFGDVKIKKIKPATKYDVINVARAMNDDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGENNPDIQSLQDALPINLQTPINKSNFRP 941
             P D NGN+IEG Y T  G+V++KK++ A  YDVI++ARAMND+FA + KELF PE EA  EA +SG+Y+  RP   P + QDCIR++  G+CFCSHLLKEH  FT +  +  C + NC CK F + P RPEE GEFWLRKRRDF    Y+ KC CKH+H +H+ +P P++C+  GCGCS F SA VCAACD+HW  H TV ETE ERK AGR I +DWLPF+ELP LRD+A+TG +NP++++L DALP +++  + +S  RP
Sbjct:   19 FPEDSNGNIIEGTYDTPVGEVRVKKLQKAKHYDVISMARAMNDNFAQRTKELFEPEVEAVKEATESGIYVSLRPNRPPHDDQDCIRVNASGRCFCSHLLKEHTSFTKNRNV-PCAKDNCKCKRFMYAPGRPEEFGEFWLRKRRDFDPKAYRMKCSCKHTHEEHECYPSPYRCRAKGCGCSAFNSASVCAACDMHWQDHDTVFETEQERKQAGRQIREDWLPFSELPALRDIAVTGVDNPNVETLADALPPSMRDKLTQSKDRP 280

[Close]

BLAST of Protein FAM221B vs. Ensembl Cavefish
Match: fam221a (family with sequence similarity 221 member A [Source:NCBI gene;Acc:103029571])

HSP 1 Score: 62.003 bits (149), Expect = 1.404e-10
Identity = 50/162 (30.86%), Postives = 70/162 (43.21%), Query Frame = 3

Query:  399 DCIRISTKGKCFCSHLLKEH----DKFTGHEPI-LKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHK--AHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGEN 863
            DC  I  +  CFCSH  K+H    ++     PI L C    C C ++ +V      SG   +R             CRCKH   DH   AH    KC      C+ F S + C  C    H H T++ET ++R+  G P+G D +P+A +  L   +   E 
Sbjct:   65 DCKLIGPETPCFCSHRFKQHQTDFEELPEETPIALPCRVQGCRCGSYQYV----HRSGSQPVR-------------CRCKHLPSDHSEAAHHLCRKCNT----CTGFLSPYTCG-CGEPGHAHVTLVETRAQREARGLPVGMD-MPYAAMGGLTGFSSLAEG 203

[Close]

BLAST of Protein FAM221B vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009126.1 (pep scaffold:Pmarinus_7.0:GL477309:63075:64646:-1 gene:ENSPMAG00000008255.1 transcript:ENSPMAT00000009126.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 120.939 bits (302), Expect = 1.755e-33
Identity = 71/181 (39.23%), Postives = 96/181 (53.04%), Query Frame = 3

Query:  342 VQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPH-KCKVSGCGCSMF-YSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGENNPDIQ 878
            V +G+Y+ +R    P    DCIR+     CFC HLL +H  +TG    + C +  C C+AFA++PSRPEE GEFWLRKR  F    ++AKCRCKHSH  H  HP     CK       MF +  + C   +   H H  +       K      G+ +LPFAE+P+LR+  +TG    D +
Sbjct:    1 VGAGVYVGWR---CPDFTWDCIRVGESCHCFCGHLLVDHHSYTGQSVHVPCAKPGCGCRAFAFIPSRPEEVGEFWLRKRPGFEPESWRAKCRCKHSHDAH--HPRGSLSCKTKEQWFRMFLHLQYHCHPLEKKHHVHNDIFFKIMPNKPQLYSTGEAYLPFAEIPKLRNAVLTGSEEDDTE 176

[Close]

BLAST of Protein FAM221B vs. Ensembl Nematostella
Match: EDO36642 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SI04])

HSP 1 Score: 218.779 bits (556), Expect = 3.365e-70
Identity = 101/222 (45.50%), Postives = 153/222 (68.92%), Query Frame = 3

Query:  222 KKIKPATKYDVINVARAMN-DDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGENNPDIQSL 884
            + I PA K +++ VARAM+ ++FAP++K+LF+PE+EA +EA+++G+YI +R    P  + DC+R+ +  KCFC HLL EH ++ G+   + C   +C CK FA++PSR E+ GE+WL++R +F    ++AKC+CKH+H +H A     +C+++GCGC  F+S F+C ACD HW  H+TV ETE  RK  G P G+ +LPF ELP LR+  +TG  + D Q +
Sbjct:   17 RPIVPAEKAELLPVARAMHREEFAPRVKKLFDPEREAALEAIETGIYIGWR---IPSFKHDCVRVGSSSKCFCGHLLSEHGRYDGNSVRVPCGMASCKCKVFAFIPSRAEDVGEYWLQRRPNFDPRSWRAKCKCKHTHEEHVA-TGMRQCRIAGCGCGRFFSNFLCCACDKHWEEHETVFETERMRKDEGIPYGEAYLPFHELPGLRNAVLTGREDDDSQYM 234

[Close]

BLAST of Protein FAM221B vs. Ensembl Nematostella
Match: EDO43591 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RY82])

HSP 1 Score: 67.3958 bits (163), Expect = 8.445e-13
Identity = 51/187 (27.27%), Postives = 86/187 (45.99%), Query Frame = 3

Query:  303 ELFNPEQ------EACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLL-----KEHDKFTGHEPI-LKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAEL 827
            +LF+PE+      +   + +++ L++ +   D      DC  +  +  CFC H L      ++       PI + C    C C+ + +VP    +                   +C+CKH   DH +   PH+C + GC C  F S+F C  C    H H+T++ET+ ERK  G P+G+D +P+A +
Sbjct:   35 KLFSPEEYEEYKKKVLPQRLKNRLFVSWTNPDG----LDCKLVGPETPCFCEHRLYVQHKTDYSSLPDTRPIPVPCKVAGCKCRHYNFVPKNGSQP-----------------IRCQCKHYTDDH-SECSPHRC-LKGCKCIAFKSSFTCG-CGFPTHEHKTIVETKEERKSRGHPVGQD-VPYAAM 196

[Close]

BLAST of Protein FAM221B vs. Planmine SMEST
Match: SMESG000035378.1 (SMESG000035378.1)

HSP 1 Score: 599.356 bits (1544), Expect = 0.000e+0
Identity = 286/287 (99.65%), Postives = 287/287 (100.00%), Query Frame = 3

Query:   90 MNKKSNIPKSKVVANKASKNPIPIDKNGNLIEGQYKTHFGDVKIKKIKPATKYDVINVARAMNDDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGENNPDIQSLQDALPINLQTPINKSNFRPIHD 950
            MNKKSNIPKSKVVANKASKNPIPIDKNGNLIEGQYKTHFGDVKIKKIKPATKYDVINVARAMNDDFAPKLKELFNPEQEACMEA+QSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGENNPDIQSLQDALPINLQTPINKSNFRPIHD
Sbjct:    1 MNKKSNIPKSKVVANKASKNPIPIDKNGNLIEGQYKTHFGDVKIKKIKPATKYDVINVARAMNDDFAPKLKELFNPEQEACMEAIQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGENNPDIQSLQDALPINLQTPINKSNFRPIHD 287

[Close]

BLAST of Protein FAM221B vs. Planmine SMEST
Match: SMESG000048037.1 (SMESG000048037.1)

HSP 1 Score: 389.423 bits (999), Expect = 5.525e-136
Identity = 172/262 (65.65%), Postives = 215/262 (82.06%), Query Frame = 3

Query:  126 VANKASKNPIPIDKNGNLIEGQYKTHFGDVKIKKIKPATKYDVINVARAMNDDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPMNQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLRDMAITGENNPDIQSLQDALPINLQ 911
            + NK  +   P D++GN+I G Y T  G+V++KK++PA  YDVINVARAMNDDFAPK+KELFNPE EACMEA+++G+YI YRP+D P N QDCIRISTKG+CFCSH L +HD FTG EP++KC+Q  C CK+FAWVPSRPEESGEFWL KRRD+ AA Y+AKCRCKH+H +H+A+PPPH+C+  GCGC +F+SAFVCAACD+HWHRH TV ETE+ERK   RP+ ++W PFAELP L  +A+TGE+NP +Q L DALP +L+
Sbjct:   24 INNKPGEPFFPTDEHGNIIPGNYSTKVGEVRVKKLEPAKAYDVINVARAMNDDFAPKVKELFNPEVEACMEAIKTGIYISYRPIDPPYNLQDCIRISTKGRCFCSHSLAQHDNFTGREPVVKCVQPGCLCKSFAWVPSRPEESGEFWLPKRRDYNAAAYRAKCRCKHTHEEHEAYPPPHRCRTKGCGCQLFHSAFVCAACDMHWHRHSTVFETEAERKRDNRPVREEWFPFAELPDLCQIALTGEDNPSVQVLADALPPHLK 285

[Close]

BLAST of Protein FAM221B vs. Planmine SMEST
Match: SMESG000019280.1 (SMESG000019280.1)

HSP 1 Score: 66.6254 bits (161), Expect = 3.973e-12
Identity = 50/174 (28.74%), Postives = 76/174 (43.68%), Query Frame = 3

Query:  306 LFNPEQEACMEAVQSGLYICYRPLDSPMNQQ--DCIRISTKGKCFCSHLLKEH----DKFTGHEPI-LKCLQMNCPCKAFAWVPSRPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGCSMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKD 806
            LF PEQ    +     + +  R   S  N Q  DCI    + +CFC H L +H    D+     PI + C    C CK++ ++                    +    +C CKH   +H A   P+ C    C CS + +++ C  C L  H+H+ ++ET+ ER   G PIG+D
Sbjct:   32 LFTPEQYEEYKRKVIPMRMANRIFISWTNPQGMDCILPGPQTECFCQHRLIQHKTDFDEMPKTRPINIPCQAGGCKCKSYHYM-----------------VKMSGQPTRCHCKHIATEH-ATSEPYLCTSKNCKCSGYKTSYTCD-CGLPAHQHRMIIETKEERVARGHPIGQD 186

The following BLAST results are available for this feature:

BLAST of Protein FAM221B vs. Ensembl Human
BLAST of Protein FAM221B vs. Ensembl Celegans
BLAST of Protein FAM221B vs. Ensembl Fly
BLAST of Protein FAM221B vs. Ensembl Zebrafish
BLAST of Protein FAM221B vs. Ensembl Xenopus
BLAST of Protein FAM221B vs. Ensembl Mouse
BLAST of Protein FAM221B vs. UniProt/SwissProt
BLAST of Protein FAM221B vs. TrEMBL
BLAST of Protein FAM221B vs. Ensembl Cavefish
BLAST of Protein FAM221B vs. Ensembl Sea Lamprey
BLAST of Protein FAM221B vs. Ensembl Yeast
BLAST of Protein FAM221B vs. Ensembl Nematostella
BLAST of Protein FAM221B vs. Ensembl Medaka
BLAST of Protein FAM221B vs. Planmine SMEST

BLAST of Protein FAM221B vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
FAM221B	4.931e-42	41.84	family with sequence similarity 221 member B [Sour...	[more]
FAM221A	1.372e-11	27.86	family with sequence similarity 221 member A [Sour...	[more]
FAM221A	1.569e-11	27.31	family with sequence similarity 221 member A [Sour...	[more]
FAM221B	1.586e-11	38.75	family with sequence similarity 221 member B [Sour...	[more]
AL133410.3	1.478e-8	44.90	Novel protein	[more]

BLAST of Protein FAM221B vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Protein FAM221B vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Protein FAM221B vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
fam221a	5.011e-10	24.57	family with sequence similarity 221 member A [Sour...	[more]
fam221a	5.011e-10	24.57	family with sequence similarity 221 member A [Sour...	[more]
fam221a	5.011e-10	24.57	family with sequence similarity 221 member A [Sour...	[more]

BLAST of Protein FAM221B vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rprd1b	3.576e-13	30.22	regulation of nuclear pre-mRNA domain containing 1...	[more]

BLAST of Protein FAM221B vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3


Match Name	E-value	Identity	Description
Fam221b	2.252e-43	37.50	family with sequence similarity 221, member B [Sou...	[more]
Fam221a	1.120e-11	27.65	family with sequence similarity 221, member A [Sou...	[more]
Fam221a	1.623e-11	27.65	family with sequence similarity 221, member A [Sou...	[more]

BLAST of Protein FAM221B vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q8C627\|F221B_MOUSE	1.575e-42	37.50	Protein FAM221B OS=Mus musculus OX=10090 GN=Fam221...	[more]
sp\|A6H8Z2\|F221B_HUMAN	2.368e-41	41.84	Protein FAM221B OS=Homo sapiens OX=9606 GN=FAM221B...	[more]
sp\|Q66HD8\|F221B_RAT	5.963e-37	40.31	Protein FAM221B OS=Rattus norvegicus OX=10116 GN=F...	[more]
sp\|Q2VPL9\|F221A_XENLA	2.837e-13	30.52	Protein FAM221A OS=Xenopus laevis OX=8355 GN=fam22...	[more]
sp\|Q28J59\|F221A_XENTR	5.061e-13	30.52	Protein FAM221A OS=Xenopus tropicalis OX=8364 GN=f...	[more]

BLAST of Protein FAM221B vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A4E0RV93	4.809e-114	58.58	Uncharacterized protein OS=Fasciola hepatica OX=61...	[more]
A0A504YHC6	7.435e-114	58.58	Uncharacterized protein OS=Fasciola gigantica OX=4...	[more]
A0A5J4P039	3.056e-111	60.64	Uncharacterized protein (Fragment) OS=Paragonimus ...	[more]
A0A4Z2DB22	1.512e-107	52.98	Uncharacterized protein OS=Schistosoma japonicum O...	[more]
A0A0X3P7W5	1.713e-106	55.89	Protein FAM221B OS=Schistocephalus solidus OX=7066...	[more]

BLAST of Protein FAM221B vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
fam221a	1.404e-10	30.86	family with sequence similarity 221 member A [Sour...	[more]

BLAST of Protein FAM221B vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000009126.1	1.755e-33	39.23	pep scaffold:Pmarinus_7.0:GL477309:63075:64646:-1 ...	[more]

BLAST of Protein FAM221B vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Protein FAM221B vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO36642	3.365e-70	45.50	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO43591	8.445e-13	27.27	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Protein FAM221B vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Protein FAM221B vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3


Match Name	E-value	Identity	Description
SMESG000035378.1	0.000e+0	99.65	SMESG000035378.1	[more]
SMESG000048037.1	5.525e-136	65.65	SMESG000048037.1	[more]
SMESG000019280.1	3.973e-12	28.74	SMESG000019280.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30013590 ID=SMED30013590|Name=Protein FAM221B|organism=Schmidtea mediterranea sexual|type=transcript|length=1042bp
AAATTGTTAATTTACAAATCTGATGGTACATAGTGTTATTTTTTTGGAAA
TTGAAATAATATACTCAGTATAAATATTACAACTGTATAATGAATAAAAA
ATCTAATATTCCAAAATCAAAAGTGGTTGCAAATAAGGCGTCTAAAAATC
CGATTCCTATAGACAAAAATGGCAATTTAATTGAAGGACAATACAAAACT
CATTTCGGAGATGTAAAAATAAAGAAAATTAAGCCGGCAACAAAATATGA
CGTAATCAATGTAGCTAGAGCAATGAATGATGATTTTGCTCCCAAACTTA
AAGAACTTTTCAATCCGGAACAAGAGGCATGTATGGAAGCTGTTCAAAGT
GGATTGTATATTTGTTATCGGCCTCTCGATTCTCCCATGAATCAACAAGA
CTGTATACGAATCTCTACTAAAGGCAAATGTTTTTGTTCTCATTTATTAA
AGGAACATGATAAGTTTACTGGTCATGAACCAATACTAAAATGTCTTCAA
ATGAATTGTCCATGTAAAGCCTTTGCTTGGGTTCCATCCCGACCGGAAGA
GTCAGGAGAATTTTGGTTACGTAAAAGAAGAGATTTTGTTGCAGCCGATT
ATAAAGCAAAATGCCGGTGTAAACACTCTCATGTCGACCACAAGGCACAT
CCTCCTCCACATAAATGTAAAGTTTCTGGTTGTGGGTGTTCGATGTTTTA
TTCGGCATTCGTGTGTGCCGCTTGTGATCTTCATTGGCATCGACATCAAA
CGGTTCTAGAAACTGAAAGCGAAAGAAAAATAGCTGGTCGGCCAATAGGA
AAAGATTGGTTACCATTTGCAGAATTACCTCAACTCAGAGACATGGCAAT
CACTGGGGAAAATAATCCAGATATTCAATCTCTACAAGATGCTCTTCCTA
TTAATTTACAGACTCCTATCAACAAATCCAATTTCAGACCTATTCATGAT
TGATAATGTTAGATTAGTATCCACAATTTGGATGAATGATCTATATTTCA
TATGATATCTATATACTGAATTTTGGTTTCACTGTATTTAAT

protein sequence of SMED30013590-orf-1

>SMED30013590-orf-1 ID=SMED30013590-orf-1|Name=SMED30013590-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=288bp
MNKKSNIPKSKVVANKASKNPIPIDKNGNLIEGQYKTHFGDVKIKKIKPA
TKYDVINVARAMNDDFAPKLKELFNPEQEACMEAVQSGLYICYRPLDSPM
NQQDCIRISTKGKCFCSHLLKEHDKFTGHEPILKCLQMNCPCKAFAWVPS
RPEESGEFWLRKRRDFVAADYKAKCRCKHSHVDHKAHPPPHKCKVSGCGC
SMFYSAFVCAACDLHWHRHQTVLETESERKIAGRPIGKDWLPFAELPQLR
DMAITGENNPDIQSLQDALPINLQTPINKSNFRPIHD*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000034	epidermis
PLANA:0000106	ciliated epithelial cell
PLANA:0000418	head
PLANA:0002089	reproductive organ

Vocabulary: **INTERPRO**
Term	Definition
IPR026755	Fam221a/b

Vocabulary: **molecular function**
Term	Definition
GO:0003674	molecular_function

Vocabulary: **cellular component**
Term	Definition
GO:0005575	cellular_component

Vocabulary: **biological process**
Term	Definition
GO:0008150	biological_process

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026755	Protein FAM221A/B	PFAM	PF14753	FAM221	coord: 101..151 e-value: 1.5E-7 score: 31.2 coord: 171..251 e-value: 1.0E-17 score: 64.4
IPR026755	Protein FAM221A/B	PANTHER	PTHR31214	FAMILY NOT NAMED	coord: 34..261
None	No IPR available	PANTHER	PTHR31214:SF3	PROTEIN FAM221B	coord: 34..261

Main menu

Protein FAM221B

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 10

Owlery

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