SMED30013577

Overview
NameSMED30013577
Smed IDSMED30013577
Length (bp)321
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30013577 (SMED30013577) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30013577 (SMED30013577) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30013577 (SMED30013577) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30013577 (SMED30013577) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30013577

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 31

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30013577h1SMcG0006787 SmedASXL_061618SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30013577h1SMcG0006787 Contig31297uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013577h1SMcG0006787 Contig31297newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013577h1SMcG0006787 Contig32555newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013577h1SMcG0006787 Contig32555uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
cephalic gangliaSMED30013577h1SMcG0006787 dd_Smed_v4_1693_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30013577h1SMcG0006787 dd_Smed_v4_1693_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30013577h1SMcG0006787 dd_Smed_v4_1693_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013577h1SMcG0006787 Contig563GPL15192PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
cephalic gangliaSMED30013577h1SMcG0006787 dd_Smed_v4_3684_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30013577h1SMcG0006787 dd_Smed_v4_3684_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30013577h1SMcG0006787 dd_Smed_v4_2265_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30013577h1SMcG0006787 dd_Smed_v4_2265_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30013577h1SMcG0006787 dd_Smed_v4_2265_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30013577h1SMcG0006787 dd_Smed_v4_2265_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30013577h1SMcG0006787 dd_Smed_v4_2265_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013577h1SMcG0019490 Contig563GPL15192PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013577h1SMcG0006787 Contig38791uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013577h1SMcG0006787 Contig38791newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013577h1SMcG0006787 Contig39149newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013577h1SMcG0006787 Contig39149uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013577h1SMcG0006788 Contig39149newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013577h1SMcG0006788 Contig39149uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30013577h1SMcG0006787 dd_Smed_v6_1693_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
pharynx musculatureSMED30013577h1SMcG0006787 dd_Smed_v6_2265_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
musculature systemSMED30013577h1SMcG0006787 dd_Smed_v6_2265_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx musculatureSMED30013577h1SMcG0006787 dd_Smed_v6_1693_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
musculature systemSMED30013577h1SMcG0006787 dd_Smed_v6_1693_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30013577h1SMcG0006787 Contig15693uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30013577h1SMcG0006787 Contig15693newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
testis primordiumSMED30013577h1SMcG0006787 Contig5941_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism juvenile stagecDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30013577 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005101:17438..17757 -1591
v31.005101:17139..17574 -855
v31.005101:17364..17680 -558
Homology
BLAST of SMED30013577 vs. TrEMBL
Match: A0A0X3PD46 (Thrombospondin-3b (Fragment) OS=Schistocephalus solidus OX=70667 GN=TSP3B PE=4 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 1.811e-31
Identity = 71/117 (60.68%), Postives = 84/117 (71.79%), Query Frame = -1
Query:   16 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNI----------------KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDS 318
            ED NGDGIPD+LE DSDGDGIPDYKEDADGDGIPDYLD+D     I                +++   + + D L +D++GDGIPD+LE DSDGDGIPDYKED DGDGIPDYL +D+
Sbjct:   24 EDANGDGIPDHLEGDSDGDGIPDYKEDADGDGIPDYLDEDANGDGIPDHLEGDSDGDGIPDYQEDEDGDGIPDYLDEDANGDGIPDHLEGDSDGDGIPDYKEDADGDGIPDYLDEDA 140          

HSP 2 Score: 90.5077 bits (223), Expect = 5.813e-21
Identity = 57/91 (62.64%), Postives = 65/91 (71.43%), Query Frame = -1
Query:    1 SDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 273
            SDGDGIPDY+ED DGDGIPDYLD                      +D++GDGIPD+LE DSDGDGIPDYKED DGDGIPDYL +D++GDGI
Sbjct:    1 SDGDGIPDYQEDEDGDGIPDYLD----------------------EDANGDGIPDHLEGDSDGDGIPDYKEDADGDGIPDYLDEDANGDGI 69          
BLAST of SMED30013577 vs. TrEMBL
Match: A0A183T0D3 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 PE=4 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 5.627e-31
Identity = 76/122 (62.30%), Postives = 89/122 (72.95%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNI----------------KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            ED NGDGIPD+LE DSDGDGIPDYKEDADGDGIPDYLD+D     I                +++   + + D + +D+DGDGIPD+LE DSDGDGIPDYKED DGDGIPDYL +D+DGDGI
Sbjct: 1384 EDANGDGIPDHLEGDSDGDGIPDYKEDADGDGIPDYLDEDANGDGIPDHLEGDSDGDGIPDYQEDEDGDGIPDYIDEDADGDGIPDHLEGDSDGDGIPDYKEDADGDGIPDYLDEDADGDGI 1505          

HSP 2 Score: 117.472 bits (293), Expect = 3.187e-28
Identity = 76/135 (56.30%), Postives = 91/135 (67.41%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNI----------------KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYL-------------QKDSDGDGI 318
            ED NGDGIPD+LE DSDGDGIPDY+ED DGDGIPDY+D+D +   I                K+++  + + D L +D+DGDGIPD+LEKDSDGDGIPDY+ED DGDGIPDYL             + DSDGDGI
Sbjct: 1422 EDANGDGIPDHLEGDSDGDGIPDYQEDEDGDGIPDYIDEDADGDGIPDHLEGDSDGDGIPDYKEDADGDGIPDYLDEDADGDGIPDHLEKDSDGDGIPDYQEDADGDGIPDYLDDDNNNDGIPDQFEGDSDGDGI 1556          

HSP 3 Score: 111.309 bits (277), Expect = 4.380e-26
Identity = 72/107 (67.29%), Postives = 81/107 (75.70%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            L DE+ DGIPD LE+DSDGDG PDY ED+D DGIPDYLDKD +   I          D+L  D DGDGIPD LE DSDGDGIPDY+EDEDGDGIPDYL +D+DG+GI
Sbjct: 1143 LSDEDEDGIPDALEQDSDGDGTPDYLEDSDDDGIPDYLDKDADGDGIP---------DDL-VDEDGDGIPDELEGDSDGDGIPDYEEDEDGDGIPDYLDEDADGNGI 1239          

HSP 4 Score: 111.309 bits (277), Expect = 4.734e-26
Identity = 72/122 (59.02%), Postives = 89/122 (72.95%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPD-------------YLDKDTESTNI---KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            ED +GDGIPD+LE DSDGDGIPDY+ED DGDGIPD             +L  D++   +   K++S  + + D L +D+DGDGIPD LE DSDGDGIPDY+EDEDGDGIPDYL +D++GDGI
Sbjct: 1270 EDADGDGIPDHLEGDSDGDGIPDYQEDEDGDGIPDYLDDDADGDGIPDHLKGDSDGDGVPDYKEDSDGDGIPDYLDEDADGDGIPDRLEGDSDGDGIPDYQEDEDGDGIPDYLDEDANGDGI 1391          

HSP 5 Score: 110.923 bits (276), Expect = 6.335e-26
Identity = 74/131 (56.49%), Postives = 85/131 (64.89%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDY-------------------------LEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            ED +G+GIPD LE DSDGDGIPDY+EDADGDGIPDYLD+D +   I          D+L+ DSDGDGIPDY                         L+ DSDGDG+PDYKED DGDGIPDYL +D+DGDGI
Sbjct: 1232 EDADGNGIPDQLEGDSDGDGIPDYEEDADGDGIPDYLDEDADGDGIP---------DHLEGDSDGDGIPDYQEDEDGDGIPDYLDDDADGDGIPDHLKGDSDGDGVPDYKEDSDGDGIPDYLDEDADGDGI 1353          

HSP 6 Score: 109.768 bits (273), Expect = 1.275e-25
Identity = 74/136 (54.41%), Postives = 91/136 (66.91%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNI----------------KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIP-------------DYLQKDSDGDGI 321
            L DE+GDGIPD LE DSDGDGIPDY+ED DGDGIPDYLD+D +   I                ++++  + + D L +D+DGDGIPD+LE DSDGDGIPDY+EDEDGDGIP             D+L+ DSDGDG+
Sbjct: 1193 LVDEDGDGIPDELEGDSDGDGIPDYEEDEDGDGIPDYLDEDADGNGIPDQLEGDSDGDGIPDYEEDADGDGIPDYLDEDADGDGIPDHLEGDSDGDGIPDYQEDEDGDGIPDYLDDDADGDGIPDHLKGDSDGDGV 1328          

HSP 7 Score: 108.997 bits (271), Expect = 2.641e-25
Identity = 72/117 (61.54%), Postives = 83/117 (70.94%), Query Frame = -1
Query:   16 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKES---SKNNVLDNLKQDSDGDGIPDYL-------------EKDSDGDGIPDYKEDEDGDGIPDYLQKDS 318
            ED +GDGIPD+LEKDSDGDGIPDY+EDADGDGIPDYLD D  +  I  +    S  + + +  +D+DGDGIPD+L             E DSDGDGIPDYKED DGDGIPDYL KDS
Sbjct: 1498 EDADGDGIPDHLEKDSDGDGIPDYQEDADGDGIPDYLDDDNNNDGIPDQFEGDSDGDGIPDYLEDTDGDGIPDFLDDDDNNNGIPDELEGDSDGDGIPDYKEDADGDGIPDYLDKDS 1614          

HSP 8 Score: 108.997 bits (271), Expect = 2.996e-25
Identity = 76/135 (56.30%), Postives = 88/135 (65.19%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNL---KQDSDGDGIPDYL-------------EKDSDGDGIPDYKEDEDGDGIPDY-------------LQKDSDGDGI 318
            ED +GDGIPD+LE DSDGDGIPDYKEDADGDGIPDYLD+D +   I     K++  D +   ++D+DGDGIPDYL             E DSDGDGIPDY ED DGDGIPD+             L+ DSDGDGI
Sbjct: 1460 EDADGDGIPDHLEGDSDGDGIPDYKEDADGDGIPDYLDEDADGDGIPDHLEKDSDGDGIPDYQEDADGDGIPDYLDDDNNNDGIPDQFEGDSDGDGIPDYLEDTDGDGIPDFLDDDDNNNGIPDELEGDSDGDGI 1594          

HSP 9 Score: 108.227 bits (269), Expect = 5.855e-25
Identity = 72/119 (60.50%), Postives = 80/119 (67.23%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGI------------PDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            LEDENG+GIPD LE D++GDGIPDY ED DGDGI            PDYLD+D     I   +       + K D DGDGIPD LE D+DGDGIPDY EDEDGDGIPDYL+ D DGDGI
Sbjct:  942 LEDENGNGIPDALEGDANGDGIPDYLEDVDGDGIKDYLEDSDNDGIPDYLDEDASIFTI---THFKQAKLSAKIDKDGDGIPDALEDDNDGDGIPDYLEDEDGDGIPDYLE-DQDGDGI 1056          

HSP 10 Score: 103.219 bits (256), Expect = 3.361e-23
Identity = 75/144 (52.08%), Postives = 86/144 (59.72%), Query Frame = -1
Query:    1 EDENGDGIPD-------------YLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDY-------------------------LEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            EDE+GDGIPD             +L+ DSDGDG+PDYKED+DGDGIPDYLD+D +   I          D L+ DSDGDGIPDY                         LE DSDGDGIPDYKED DGDGIPDYL +D++GDGI
Sbjct: 1295 EDEDGDGIPDYLDDDADGDGIPDHLKGDSDGDGVPDYKEDSDGDGIPDYLDEDADGDGIP---------DRLEGDSDGDGIPDYQEDEDGDGIPDYLDEDANGDGIPDHLEGDSDGDGIPDYKEDADGDGIPDYLDEDANGDGI 1429          

HSP 11 Score: 92.0485 bits (227), Expect = 2.170e-19
Identity = 62/115 (53.91%), Postives = 79/115 (68.70%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKK--------ESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
             +D +G+GIPD  E DSD DGIPDY ED+D DGIPD +D+D       K        +S  + ++D+L +D +G+GIPD LE D++GDGIPDY ED DGDGI DYL+ DSD DGI
Sbjct:  875 FKDSDGNGIPDACEGDSDKDGIPDYLEDSDNDGIPDCIDEDPHPVKKGKPPKISRFVDSDGDGIMDHL-EDENGNGIPDALEGDANGDGIPDYLEDVDGDGIKDYLE-DSDNDGI 987          

HSP 12 Score: 89.7373 bits (221), Expect = 1.686e-18
Identity = 67/125 (53.60%), Postives = 82/125 (65.60%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTE-------STNIKKESSKNNVLDNLKQDSDGDG------------IPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            +D+N +GIPDYLE D D DG+P+Y ED D DGIPD++DK            N+  E  ++ + D L+QDSDGDG            IPDYL+KD+DGDGIPD   DEDGDGIPD L+ DSDGDGI
Sbjct: 1092 QDKNDNGIPDYLE-DWDEDGVPNYLEDEDNDGIPDFMDKKDNRKNKKRFRVNLSDED-EDGIPDALEQDSDGDGTPDYLEDSDDDGIPDYLDKDADGDGIPDDLVDEDGDGIPDELEGDSDGDGI 1214          

HSP 13 Score: 86.2705 bits (212), Expect = 2.743e-17
Identity = 73/168 (43.45%), Postives = 85/168 (50.60%), Query Frame = -1
Query:    1 LEDEN-GDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTN---------------------IKKESSKNNVLDNLK-----------QDSDGDGIPDY----------------------------LEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            LED+N GDGIPDYLE D DGDGIPDY ED DGDGIP+YLD D E                         +++ + N + D L+           +D D DGIPD+                            LE+DSDGDG PDY ED D DGIPDYL KD+DGDGI
Sbjct: 1023 LEDDNDGDGIPDYLE-DEDGDGIPDYLEDQDGDGIPNYLDSDEEKPGGGRPMLSAEDEDDDKDTTDKIFEQDKNDNGIPDYLEDWDEDGVPNYLEDEDNDGIPDFMDKKDNRKNKKRFRVNLSDEDEDGIPDALEQDSDGDGTPDYLEDSDDDGIPDYLDKDADGDGI 1189          

HSP 14 Score: 84.3445 bits (207), Expect = 1.069e-16
Identity = 54/102 (52.94%), Postives = 72/102 (70.59%), Query Frame = -1
Query:   16 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDS 321
            LED++GDG+PD+LE D D +G+P++ ED D DGIP+YLDK + +  +KK   + +       DSD DGI D +E D D DGIP++ ED DGDGIP+YL KDS
Sbjct:  764 LEDDDGDGVPDFLE-DDDNNGVPNFLEDNDNDGIPNYLDK-SPNKIVKKPKRRPHAKKLEVADSDNDGISDMME-DDDNDGIPNFLEDTDGDGIPNYLDKDS 862          

HSP 15 Score: 80.8777 bits (198), Expect = 1.774e-15
Identity = 54/101 (53.47%), Postives = 70/101 (69.31%), Query Frame = -1
Query:   19 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKD 321
            + D + DGI D +E D D DGIP++ ED DGDGIP+YLDKD+ +  +KK+   ++      +DSDG+GIPD  E DSD DGIPDY ED D DGIPD + +D
Sbjct:  822 VADSDNDGISDMME-DDDNDGIPNFLEDTDGDGIPNYLDKDS-AYYMKKKGKLSSF-----KDSDGNGIPDACEGDSDKDGIPDYLEDSDNDGIPDCIDED 915          

HSP 16 Score: 79.7221 bits (195), Expect = 4.645e-15
Identity = 60/129 (46.51%), Postives = 78/129 (60.47%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGI------------PDYLDKDTESTNIKK----------ESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            L DE+GD IPD LE D D DG+ DY  D D DGI            P+ LD+D+    IKK          +  ++ V D L+ D DGDGIPDYLE D DGDG+PD+ ED+D +G+P++L+ D+D DGI
Sbjct:  670 LLDEDGDDIPDVLEDDQDSDGVADYLRDKDNDGIRDYYEDDDGDGVPNMLDRDSPLFLIKKLIKWKMPKEMDRDRDGVPDYLQGDKDGDGIPDYLE-DDDGDGVPDFLEDDDNNGVPNFLE-DNDNDGI 796          

HSP 17 Score: 76.6406 bits (187), Expect = 5.817e-14
Identity = 66/141 (46.81%), Postives = 79/141 (56.03%), Query Frame = -1
Query:    1 LEDENGDGIPDYL-------------EKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLE-KDSDGD-------------------GIPDYKEDEDGDGIPD-YLQKDSDGDGI 321
            LED +GDGIPD+L             E DSDGDGIPDYKEDADGDGIPDYLDKD+ S      ++    L     D++ +G PD LE KD+DGD                   GI D  ED++ +GI D + QKDSD DGI
Sbjct: 1560 LEDTDGDGIPDFLDDDDNNNGIPDELEGDSDGDGIPDYKEDADGDGIPDYLDKDSRSYRPPNPTT----LHRRVFDANANGRPDILELKDTDGDGIIDLYDTDDDNDGIPDEEGILDVDEDDNKNGILDKHEQKDSDKDGI 1696          

HSP 18 Score: 71.633 bits (174), Expect = 3.555e-12
Identity = 48/80 (60.00%), Postives = 56/80 (70.00%), Query Frame = -1
Query:   40 KDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGI 279
            KDSDGDGIPDY EDADGDGIP+YLD D+ S + K+ S    ++  L  D DGD IPD LE D D DG+ DY  D+D DGI
Sbjct:  627 KDSDGDGIPDYLEDADGDGIPNYLDDDSPSFS-KRFSYYMKLVKLL--DEDGDDIPDVLEDDQDSDGVADYLRDKDNDGI 703          

HSP 19 Score: 67.0106 bits (162), Expect = 1.273e-10
Identity = 57/154 (37.01%), Postives = 75/154 (48.70%), Query Frame = -1
Query:   19 EDENGDGIPDYLEKDSDGDGIPDY---------------------------------KEDADGDGIPDYL-DKDTESTNIKKESSKNNVLDNL--------------------KQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKD 318
            +D +GDGIPDYLE D+DGDGIP+Y                                 ++D D DG+ DYL DKD +      E    + + N+                    + D D DG+PDYL+ D DGDGIPDY ED+DGDG+PD+L+ D
Sbjct:  627 KDSDGDGIPDYLE-DADGDGIPNYLDDDSPSFSKRFSYYMKLVKLLDEDGDDIPDVLEDDQDSDGVADYLRDKDNDGIRDYYEDDDGDGVPNMLDRDSPLFLIKKLIKWKMPKEMDRDRDGVPDYLQGDKDGDGIPDYLEDDDGDGVPDFLEDD 779          
BLAST of SMED30013577 vs. TrEMBL
Match: A0A3P7CSP7 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS9931 PE=4 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 5.682e-31
Identity = 76/122 (62.30%), Postives = 89/122 (72.95%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNI----------------KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            ED NGDGIPD+LE DSDGDGIPDYKEDADGDGIPDYLD+D     I                +++   + + D + +D+DGDGIPD+LE DSDGDGIPDYKED DGDGIPDYL +D+DGDGI
Sbjct: 1384 EDANGDGIPDHLEGDSDGDGIPDYKEDADGDGIPDYLDEDANGDGIPDHLEGDSDGDGIPDYQEDEDGDGIPDYIDEDADGDGIPDHLEGDSDGDGIPDYKEDADGDGIPDYLDEDADGDGI 1505          

HSP 2 Score: 117.472 bits (293), Expect = 3.219e-28
Identity = 76/135 (56.30%), Postives = 91/135 (67.41%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNI----------------KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYL-------------QKDSDGDGI 318
            ED NGDGIPD+LE DSDGDGIPDY+ED DGDGIPDY+D+D +   I                K+++  + + D L +D+DGDGIPD+LEKDSDGDGIPDY+ED DGDGIPDYL             + DSDGDGI
Sbjct: 1422 EDANGDGIPDHLEGDSDGDGIPDYQEDEDGDGIPDYIDEDADGDGIPDHLEGDSDGDGIPDYKEDADGDGIPDYLDEDADGDGIPDHLEKDSDGDGIPDYQEDADGDGIPDYLDDDNNNDGIPDQFEGDSDGDGI 1556          

HSP 3 Score: 111.309 bits (277), Expect = 4.466e-26
Identity = 72/107 (67.29%), Postives = 81/107 (75.70%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            L DE+ DGIPD LE+DSDGDG PDY ED+D DGIPDYLDKD +   I          D+L  D DGDGIPD LE DSDGDGIPDY+EDEDGDGIPDYL +D+DG+GI
Sbjct: 1143 LSDEDEDGIPDALEQDSDGDGTPDYLEDSDDDGIPDYLDKDADGDGIP---------DDL-VDEDGDGIPDELEGDSDGDGIPDYEEDEDGDGIPDYLDEDADGNGI 1239          

HSP 4 Score: 111.309 bits (277), Expect = 4.780e-26
Identity = 72/122 (59.02%), Postives = 89/122 (72.95%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPD-------------YLDKDTESTNI---KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            ED +GDGIPD+LE DSDGDGIPDY+ED DGDGIPD             +L  D++   +   K++S  + + D L +D+DGDGIPD LE DSDGDGIPDY+EDEDGDGIPDYL +D++GDGI
Sbjct: 1270 EDADGDGIPDHLEGDSDGDGIPDYQEDEDGDGIPDYLDDDADGDGIPDHLKGDSDGDGVPDYKEDSDGDGIPDYLDEDADGDGIPDRLEGDSDGDGIPDYQEDEDGDGIPDYLDEDANGDGI 1391          

HSP 5 Score: 110.923 bits (276), Expect = 6.396e-26
Identity = 74/131 (56.49%), Postives = 85/131 (64.89%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDY-------------------------LEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            ED +G+GIPD LE DSDGDGIPDY+EDADGDGIPDYLD+D +   I          D+L+ DSDGDGIPDY                         L+ DSDGDG+PDYKED DGDGIPDYL +D+DGDGI
Sbjct: 1232 EDADGNGIPDQLEGDSDGDGIPDYEEDADGDGIPDYLDEDADGDGIP---------DHLEGDSDGDGIPDYQEDEDGDGIPDYLDDDADGDGIPDHLKGDSDGDGVPDYKEDSDGDGIPDYLDEDADGDGI 1353          

HSP 6 Score: 109.768 bits (273), Expect = 1.287e-25
Identity = 74/136 (54.41%), Postives = 91/136 (66.91%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNI----------------KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIP-------------DYLQKDSDGDGI 321
            L DE+GDGIPD LE DSDGDGIPDY+ED DGDGIPDYLD+D +   I                ++++  + + D L +D+DGDGIPD+LE DSDGDGIPDY+EDEDGDGIP             D+L+ DSDGDG+
Sbjct: 1193 LVDEDGDGIPDELEGDSDGDGIPDYEEDEDGDGIPDYLDEDADGNGIPDQLEGDSDGDGIPDYEEDADGDGIPDYLDEDADGDGIPDHLEGDSDGDGIPDYQEDEDGDGIPDYLDDDADGDGIPDHLKGDSDGDGV 1328          

HSP 7 Score: 108.997 bits (271), Expect = 2.666e-25
Identity = 72/117 (61.54%), Postives = 83/117 (70.94%), Query Frame = -1
Query:   16 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKES---SKNNVLDNLKQDSDGDGIPDYL-------------EKDSDGDGIPDYKEDEDGDGIPDYLQKDS 318
            ED +GDGIPD+LEKDSDGDGIPDY+EDADGDGIPDYLD D  +  I  +    S  + + +  +D+DGDGIPD+L             E DSDGDGIPDYKED DGDGIPDYL KDS
Sbjct: 1498 EDADGDGIPDHLEKDSDGDGIPDYQEDADGDGIPDYLDDDNNNDGIPDQFEGDSDGDGIPDYLEDTDGDGIPDFLDDDDNNNGIPDELEGDSDGDGIPDYKEDADGDGIPDYLDKDS 1614          

HSP 8 Score: 108.997 bits (271), Expect = 3.055e-25
Identity = 76/135 (56.30%), Postives = 88/135 (65.19%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNL---KQDSDGDGIPDYL-------------EKDSDGDGIPDYKEDEDGDGIPDY-------------LQKDSDGDGI 318
            ED +GDGIPD+LE DSDGDGIPDYKEDADGDGIPDYLD+D +   I     K++  D +   ++D+DGDGIPDYL             E DSDGDGIPDY ED DGDGIPD+             L+ DSDGDGI
Sbjct: 1460 EDADGDGIPDHLEGDSDGDGIPDYKEDADGDGIPDYLDEDADGDGIPDHLEKDSDGDGIPDYQEDADGDGIPDYLDDDNNNDGIPDQFEGDSDGDGIPDYLEDTDGDGIPDFLDDDDNNNGIPDELEGDSDGDGI 1594          

HSP 9 Score: 107.842 bits (268), Expect = 6.028e-25
Identity = 72/119 (60.50%), Postives = 80/119 (67.23%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGI------------PDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            LEDENG+GIPD LE D++GDGIPDY ED DGDGI            PDYLD+D     I   +       + K D DGDGIPD LE D+DGDGIPDY EDEDGDGIPDYL+ D DGDGI
Sbjct:  942 LEDENGNGIPDALEGDANGDGIPDYLEDVDGDGIKDYLEDSDNDGIPDYLDEDASIFTI---THFKQAKLSAKIDKDGDGIPDALEDDNDGDGIPDYLEDEDGDGIPDYLE-DQDGDGI 1056          

HSP 10 Score: 102.834 bits (255), Expect = 3.394e-23
Identity = 75/144 (52.08%), Postives = 86/144 (59.72%), Query Frame = -1
Query:    1 EDENGDGIPD-------------YLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDY-------------------------LEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            EDE+GDGIPD             +L+ DSDGDG+PDYKED+DGDGIPDYLD+D +   I          D L+ DSDGDGIPDY                         LE DSDGDGIPDYKED DGDGIPDYL +D++GDGI
Sbjct: 1295 EDEDGDGIPDYLDDDADGDGIPDHLKGDSDGDGVPDYKEDSDGDGIPDYLDEDADGDGIP---------DRLEGDSDGDGIPDYQEDEDGDGIPDYLDEDANGDGIPDHLEGDSDGDGIPDYKEDADGDGIPDYLDEDANGDGI 1429          

HSP 11 Score: 92.0485 bits (227), Expect = 2.191e-19
Identity = 62/115 (53.91%), Postives = 79/115 (68.70%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKK--------ESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
             +D +G+GIPD  E DSD DGIPDY ED+D DGIPD +D+D       K        +S  + ++D+L +D +G+GIPD LE D++GDGIPDY ED DGDGI DYL+ DSD DGI
Sbjct:  875 FKDSDGNGIPDACEGDSDKDGIPDYLEDSDNDGIPDCIDEDPHPVKKGKPPKISRFVDSDGDGIMDHL-EDENGNGIPDALEGDANGDGIPDYLEDVDGDGIKDYLE-DSDNDGI 987          

HSP 12 Score: 89.7373 bits (221), Expect = 1.719e-18
Identity = 67/125 (53.60%), Postives = 82/125 (65.60%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTE-------STNIKKESSKNNVLDNLKQDSDGDG------------IPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            +D+N +GIPDYLE D D DG+P+Y ED D DGIPD++DK            N+  E  ++ + D L+QDSDGDG            IPDYL+KD+DGDGIPD   DEDGDGIPD L+ DSDGDGI
Sbjct: 1092 QDKNDNGIPDYLE-DWDEDGVPNYLEDEDNDGIPDFMDKKDNRKNKKRFRVNLSDED-EDGIPDALEQDSDGDGTPDYLEDSDDDGIPDYLDKDADGDGIPDDLVDEDGDGIPDELEGDSDGDGI 1214          

HSP 13 Score: 86.2705 bits (212), Expect = 2.796e-17
Identity = 73/168 (43.45%), Postives = 85/168 (50.60%), Query Frame = -1
Query:    1 LEDEN-GDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTN---------------------IKKESSKNNVLDNLK-----------QDSDGDGIPDY----------------------------LEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            LED+N GDGIPDYLE D DGDGIPDY ED DGDGIP+YLD D E                         +++ + N + D L+           +D D DGIPD+                            LE+DSDGDG PDY ED D DGIPDYL KD+DGDGI
Sbjct: 1023 LEDDNDGDGIPDYLE-DEDGDGIPDYLEDQDGDGIPNYLDSDEEKPGGGRPMLSAEDEDDDKDTTDKIFEQDKNDNGIPDYLEDWDEDGVPNYLEDEDNDGIPDFMDKKDNRKNKKRFRVNLSDEDEDGIPDALEQDSDGDGTPDYLEDSDDDGIPDYLDKDADGDGI 1189          

HSP 14 Score: 84.3445 bits (207), Expect = 1.090e-16
Identity = 54/102 (52.94%), Postives = 72/102 (70.59%), Query Frame = -1
Query:   16 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDS 321
            LED++GDG+PD+LE D D +G+P++ ED D DGIP+YLDK + +  +KK   + +       DSD DGI D +E D D DGIP++ ED DGDGIP+YL KDS
Sbjct:  764 LEDDDGDGVPDFLE-DDDNNGVPNFLEDNDNDGIPNYLDK-SPNKIVKKPKRRPHAKKLEVADSDNDGISDMME-DDDNDGIPNFLEDTDGDGIPNYLDKDS 862          

HSP 15 Score: 80.8777 bits (198), Expect = 1.827e-15
Identity = 54/101 (53.47%), Postives = 70/101 (69.31%), Query Frame = -1
Query:   19 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKD 321
            + D + DGI D +E D D DGIP++ ED DGDGIP+YLDKD+ +  +KK+   ++      +DSDG+GIPD  E DSD DGIPDY ED D DGIPD + +D
Sbjct:  822 VADSDNDGISDMME-DDDNDGIPNFLEDTDGDGIPNYLDKDS-AYYMKKKGKLSSF-----KDSDGNGIPDACEGDSDKDGIPDYLEDSDNDGIPDCIDED 915          

HSP 16 Score: 79.7221 bits (195), Expect = 4.736e-15
Identity = 60/129 (46.51%), Postives = 78/129 (60.47%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGI------------PDYLDKDTESTNIKK----------ESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            L DE+GD IPD LE D D DG+ DY  D D DGI            P+ LD+D+    IKK          +  ++ V D L+ D DGDGIPDYLE D DGDG+PD+ ED+D +G+P++L+ D+D DGI
Sbjct:  670 LLDEDGDDIPDVLEDDQDSDGVADYLRDKDNDGIRDYYEDDDGDGVPNMLDRDSPLFLIKKLIKWKMPKEMDRDRDGVPDYLQGDKDGDGIPDYLE-DDDGDGVPDFLEDDDNNGVPNFLE-DNDNDGI 796          

HSP 17 Score: 76.6406 bits (187), Expect = 5.761e-14
Identity = 66/141 (46.81%), Postives = 79/141 (56.03%), Query Frame = -1
Query:    1 LEDENGDGIPDYL-------------EKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLE-KDSDGD-------------------GIPDYKEDEDGDGIPD-YLQKDSDGDGI 321
            LED +GDGIPD+L             E DSDGDGIPDYKEDADGDGIPDYLDKD+ S      ++    L     D++ +G PD LE KD+DGD                   GI D  ED++ +GI D + QKDSD DGI
Sbjct: 1560 LEDTDGDGIPDFLDDDDNNNGIPDELEGDSDGDGIPDYKEDADGDGIPDYLDKDSRSYRPPNPTT----LHRRVFDANANGRPDILELKDTDGDGIIDLYDTDDDNDGIPDEEGILDVDEDDNKNGILDKHEQKDSDKDGI 1696          

HSP 18 Score: 71.633 bits (174), Expect = 3.625e-12
Identity = 48/80 (60.00%), Postives = 56/80 (70.00%), Query Frame = -1
Query:   40 KDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGI 279
            KDSDGDGIPDY EDADGDGIP+YLD D+ S + K+ S    ++  L  D DGD IPD LE D D DG+ DY  D+D DGI
Sbjct:  627 KDSDGDGIPDYLEDADGDGIPNYLDDDSPSFS-KRFSYYMKLVKLL--DEDGDDIPDVLEDDQDSDGVADYLRDKDNDGI 703          

HSP 19 Score: 67.0106 bits (162), Expect = 1.298e-10
Identity = 57/154 (37.01%), Postives = 75/154 (48.70%), Query Frame = -1
Query:   19 EDENGDGIPDYLEKDSDGDGIPDY---------------------------------KEDADGDGIPDYL-DKDTESTNIKKESSKNNVLDNL--------------------KQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKD 318
            +D +GDGIPDYLE D+DGDGIP+Y                                 ++D D DG+ DYL DKD +      E    + + N+                    + D D DG+PDYL+ D DGDGIPDY ED+DGDG+PD+L+ D
Sbjct:  627 KDSDGDGIPDYLE-DADGDGIPNYLDDDSPSFSKRFSYYMKLVKLLDEDGDDIPDVLEDDQDSDGVADYLRDKDNDGIRDYYEDDDGDGVPNMLDRDSPLFLIKKLIKWKMPKEMDRDRDGVPDYLQGDKDGDGIPDYLEDDDGDGVPDFLEDD 779          
BLAST of SMED30013577 vs. TrEMBL
Match: A0A504YRT1 (Septin-10 OS=Fasciola gigantica OX=46835 GN=FGIG_08010 PE=4 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 3.859e-30
Identity = 75/105 (71.43%), Postives = 83/105 (79.05%), Query Frame = -1
Query:    1 DENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 315
            D +GDGIPDYLE DSDGDGIPDY ED+DGDGIPDYLD DT+S  + K + K  +L    QD D DGIPD LE DSDGDGIPDY+ED DGDGIPDYL+ D DGDGI
Sbjct: 1007 DSDGDGIPDYLE-DSDGDGIPDYLEDSDGDGIPDYLDSDTKSQFMPK-TLKQKLLKRTYQDLDNDGIPDALEGDSDGDGIPDYQEDTDGDGIPDYLE-DEDGDGI 1108          

HSP 2 Score: 115.546 bits (288), Expect = 1.427e-27
Identity = 73/107 (68.22%), Postives = 84/107 (78.50%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYL-DKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            EDE+GDGIPD+LE DSDGDGIPDY ED DGDGIPDYL D+D +      +   + +   L+ D+DGDGIPD+LE DSDGDGIPDY ED DGDGIPDYL+ DSDGDGI
Sbjct:  932 EDEDGDGIPDHLEGDSDGDGIPDYLEDEDGDGIPDYLEDEDGDGIPDYLDDDSSYMRGKLQVDTDGDGIPDHLEGDSDGDGIPDYLEDSDGDGIPDYLE-DSDGDGI 1037          

HSP 3 Score: 113.62 bits (283), Expect = 6.063e-27
Identity = 75/121 (61.98%), Postives = 87/121 (71.90%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYL------------DKDTESTNIKKESSKNNVLDNLKQ--DSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            L D +GDGIPD+LE DSDGDGI DY+ED DGDGIPDYL            D+D+E+    +   K   L+ ++Q  D+DGDGIPDYLE DSDGDGIPDY ED DGDGIPDYL+ DSDGDGI
Sbjct: 1218 LNDLDGDGIPDHLEGDSDGDGILDYQEDTDGDGIPDYLEDSDEDGIPDYMDEDSETPFSPRVFRK---LEEIRQREDADGDGIPDYLEGDSDGDGIPDYLEDADGDGIPDYLE-DSDGDGI 1334          

HSP 4 Score: 109.383 bits (272), Expect = 2.079e-25
Identity = 67/106 (63.21%), Postives = 84/106 (79.25%), Query Frame = -1
Query:   13 DENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIK-----KESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSD 315
            D +GDGIPDYLE D+DGDGIPDY ED+DGDGIPDYLD+D+  +  K     ++ +KN + D+L++DSD DG PDYLE D+D DGIPDY ED DGDGIPDYL +D++
Sbjct: 1304 DSDGDGIPDYLE-DADGDGIPDYLEDSDGDGIPDYLDEDSALSKSKSVFRIEDKNKNGIPDHLERDSDYDGTPDYLE-DTDKDGIPDYLEDTDGDGIPDYLDEDAN 1407          

HSP 5 Score: 108.227 bits (269), Expect = 4.839e-25
Identity = 64/105 (60.95%), Postives = 81/105 (77.14%), Query Frame = -1
Query:    1 DENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 315
            D +GDG+ DYLE DSDGDGIPDY ED+DGDGIPDY+D D  +T   + + +  +L    +D+DGDGIPD+L+ DSDGDG+ D+ ED DGDG PDYL+ DSDGDG+
Sbjct: 1510 DSDGDGVADYLE-DSDGDGIPDYLEDSDGDGIPDYIDDDA-TTQFSQRNYRKLMLKTQFEDNDGDGIPDHLQGDSDGDGVLDFLEDSDGDGTPDYLE-DSDGDGV 1611          

HSP 6 Score: 103.99 bits (258), Expect = 1.341e-23
Identity = 67/119 (56.30%), Postives = 78/119 (65.55%), Query Frame = -1
Query:    1 LEDENGDGIPDYLE------------KDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
              D + DGIPD+LE            +DSDGD IPDY ED+DGDGIPDYLD+D  +T       + +       D DGDGIPD+LE DSDGDG+ DY ED DGDGIPDYL+ DSDGDGI
Sbjct: 1424 FADADQDGIPDHLEGDLDRNGVLDYLEDSDGDSIPDYLEDSDGDGIPDYLDEDA-TTKFAPRVLRKSRPQRHYADVDGDGIPDHLEGDSDGDGVADYLEDSDGDGIPDYLE-DSDGDGI 1540          

HSP 7 Score: 103.219 bits (256), Expect = 2.944e-23
Identity = 66/116 (56.90%), Postives = 80/116 (68.97%), Query Frame = -1
Query:   19 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGD------------GIPDYLDKDTESTNIKKESSKNN---VLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKD 321
             ED +GDGIPD+L+ DSDGDG+ D+ ED+DGD            G+PDYLD+D  S    K  +  N   + D+L+ DSDGDGIPDYLE D+DGDGIPDY ED DGDGIP+YL KD
Sbjct: 1566 FEDNDGDGIPDHLQGDSDGDGVLDFLEDSDGDGTPDYLEDSDGDGVPDYLDEDANSQFSPKHFTDKNRDGIPDSLEGDSDGDGIPDYLE-DTDGDGIPDYLEDSDGDGIPNYLDKD 1680          

HSP 8 Score: 101.679 bits (252), Expect = 1.031e-22
Identity = 73/135 (54.07%), Postives = 86/135 (63.70%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYK---------------------------EDADGDGIPDYLDKDTEST-NIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            ++D +GDGIPDYLE DSDGDGIPDY+                           ED DGDGIPDYLD+D  S  + K+  S+   L++L    DGDGIPD+LE DSDGDGI DY+ED DGDGIPDYL+ DSD DGI
Sbjct: 1134 VDDLDGDGIPDYLEGDSDGDGIPDYQEDTDGDDGDGIPDYQEDTDGDGIPDYLEDEDGDGIPDYLDEDANSQFSAKRLKSQKMKLNDL----DGDGIPDHLEGDSDGDGILDYQEDTDGDGIPDYLE-DSDEDGI 1263          

HSP 9 Score: 99.7525 bits (247), Expect = 5.221e-22
Identity = 72/133 (54.14%), Postives = 80/133 (60.15%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDA------------DGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDG---------------IPDYKEDEDGDGIPDYLQKDSDGDGI 318
            +D + DGIPD LE DSDGDGIPDY+ED             DGDGIPDYLD+D  S    K   K  +   +  D DGDGIPDYLE DSDGDG               IPDY+ED DGDGIPDYL+ D DGDGI
Sbjct: 1064 QDLDNDGIPDALEGDSDGDGIPDYQEDTDGDGIPDYLEDEDGDGIPDYLDEDANSQFSAKRIRKMKLQKKV-DDLDGDGIPDYLEGDSDGDGIPDYQEDTDGDDGDGIPDYQEDTDGDGIPDYLE-DEDGDGI 1194          

HSP 10 Score: 97.0561 bits (240), Expect = 4.736e-21
Identity = 63/119 (52.94%), Postives = 77/119 (64.71%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDS------------DGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            +ED+N +GIPD+LE+DS            D DGIPDY ED DGDGIPDYLD+D  +T    +  +         D+D DGIPD+LE D D +G+ DY ED DGD IPDYL+ DSDGDGI
Sbjct: 1353 IEDKNKNGIPDHLERDSDYDGTPDYLEDTDKDGIPDYLEDTDGDGIPDYLDEDA-NTQFSPKKIRGRARGKKFADADQDGIPDHLEGDLDRNGVLDYLEDSDGDSIPDYLE-DSDGDGI 1469          

HSP 11 Score: 95.9005 bits (237), Expect = 1.082e-20
Identity = 61/110 (55.45%), Postives = 75/110 (68.18%), Query Frame = -1
Query:   22 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLD----------KDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQK 321
              D+N DGIPD LE DSDGDGIPDY ED DGDGIPDYL+           D +  ++K E+    VL+  + DS+ +GIPDYLE D DGDG+P+Y +DEDGDGI D+L K
Sbjct: 1628 FTDKNRDGIPDSLEGDSDGDGIPDYLEDTDGDGIPDYLEDSDGDGIPNYLDKDVVDVKVEA----VLEGFEADSNKNGIPDYLE-DWDGDGVPNYLDDEDGDGIADFLDK 1732          

HSP 12 Score: 89.3521 bits (220), Expect = 1.847e-18
Identity = 53/96 (55.21%), Postives = 68/96 (70.83%), Query Frame = -1
Query:   28 DENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYL 315
            D N +GIPDYLE D DGDG+P+Y +D DGDGI D+LDK     N  K+    N+   +  D++ +GIPD+LE DSDGDG+ DY++D D DGIPDYL
Sbjct: 1696 DSNKNGIPDYLE-DWDGDGVPNYLDDEDGDGIADFLDK---KDNRIKDGKMPNLKRKITNDANANGIPDHLEGDSDGDGVLDYRQDSDKDGIPDYL 1787          

HSP 13 Score: 83.5741 bits (205), Expect = 2.019e-16
Identity = 57/120 (47.50%), Postives = 72/120 (60.00%), Query Frame = -1
Query:    1 DENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDG---------------IPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 315
            D N +GIPD+LE DSDGDG+ DY++D+D DGIPDYLD D      +K  SK  V    K++ +                  +P  L  D DGDGIPD ++D DGDGIPD+L  D+DGDGI
Sbjct: 1752 DANANGIPDHLEGDSDGDGVLDYRQDSDKDGIPDYLDDDDPQAKFRKPKSKPKVKLTGKKNVEKPAQVIPSKPKKSKKVIKVPPKL--DLDGDGIPDDQKDTDGDGIPDFLDSDADGDGI 1869          

HSP 14 Score: 68.1662 bits (165), Expect = 5.025e-11
Identity = 38/50 (76.00%), Postives = 42/50 (84.00%), Query Frame = -1
Query:    1 DNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 150
            +   +D DGDGIPD+LE DSDGDGIPDY EDEDGDGIPDYL+ D DGDGI
Sbjct:  928 ERFTEDEDGDGIPDHLEGDSDGDGIPDYLEDEDGDGIPDYLE-DEDGDGI 976          

HSP 15 Score: 53.9138 bits (128), Expect = 5.134e-6
Identity = 48/113 (42.48%), Postives = 62/113 (54.87%), Query Frame = -1
Query:    1 DSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLE-KDS-------------------DGDGIPDYKEDEDGDGIPDYLQ-KDSDGDGI 276
            D DGDGIPD ++D DGDGIPD+LD D +   I  +  K         D + +G+PD+LE KD+                   D +GI D  EDE+ + IPDYL+  DSDGDGI
Sbjct: 1838 DLDGDGIPDDQKDTDGDGIPDFLDSDADGDGIPDDQIK-------VVDRNRNGVPDHLELKDTDGDGIIDLLDDDDDNDGIPDDEGIADVLEDENQNQIPDYLEMTDSDGDGI 1943          
BLAST of SMED30013577 vs. TrEMBL
Match: A0A3P7MJ55 (Uncharacterized protein (Fragment) OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS13054 PE=4 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 6.392e-30
Identity = 75/122 (61.48%), Postives = 91/122 (74.59%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNI----------------KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            ED +G+GIPDYLE DSDGDGIPDY+EDADGDGIPDYLD+D +   I                ++++  + + D L +D+DGDGIPD LE DSDGDGIPDY+EDEDGDGIPDYL +D+DGDGI
Sbjct:  869 EDADGNGIPDYLEGDSDGDGIPDYEEDADGDGIPDYLDEDADGDGIPDHLEGDSDGDGIPDYQEDADGDGIPDYLDEDADGDGIPDNLEGDSDGDGIPDYEEDEDGDGIPDYLDEDADGDGI 990          

HSP 2 Score: 121.324 bits (303), Expect = 1.133e-29
Identity = 74/122 (60.66%), Postives = 90/122 (73.77%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNI----------------KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            ED +GDGIPD+LE DSDGDGIPDY+EDADGDGIPDYLD+D +   I                +++   + + D L +D+DGDGIPD+LE DSDGDGIPDY+ED DGDGIPDYL +D+DGDGI
Sbjct:  907 EDADGDGIPDHLEGDSDGDGIPDYQEDADGDGIPDYLDEDADGDGIPDNLEGDSDGDGIPDYEEDEDGDGIPDYLDEDADGDGIPDHLEGDSDGDGIPDYQEDADGDGIPDYLDEDADGDGI 1028          

HSP 3 Score: 118.242 bits (295), Expect = 1.401e-28
Identity = 75/122 (61.48%), Postives = 89/122 (72.95%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNL---KQDSDGDGIPDYL-------------EKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            ED +GDGIPD+LE DSDGDGIPDY+EDADGDGIPDYLD+D +   I     K++  D +   ++D+DGDGIPDYL             E DSDGDG+PDYKED DGDGIPDYL +D+DGDGI
Sbjct:  983 EDADGDGIPDHLEGDSDGDGIPDYQEDADGDGIPDYLDEDADGDGIPDHLEKDSDGDGIPDYEEDADGDGIPDYLDDDDNNDGIPDHLEGDSDGDGVPDYKEDADGDGIPDYLDEDADGDGI 1104          

HSP 4 Score: 112.464 bits (280), Expect = 1.729e-26
Identity = 70/113 (61.95%), Postives = 83/113 (73.45%), Query Frame = -1
Query:   28 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNI----------------KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYL 318
            ED +GDGIPD+LEKDSDGDGIPDY+EDADGDGIPDYLD D  +  I                K+++  + + D L +D+DGDGIPD+LE DSDGDGIPDY+ED DGDGIPDYL
Sbjct: 1021 EDADGDGIPDHLEKDSDGDGIPDYEEDADGDGIPDYLDDDDNNDGIPDHLEGDSDGDGVPDYKEDADGDGIPDYLDEDADGDGIPDHLEGDSDGDGIPDYQEDADGDGIPDYL 1133          

HSP 5 Score: 111.694 bits (278), Expect = 2.837e-26
Identity = 72/122 (59.02%), Postives = 87/122 (71.31%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNI----------------KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            ED +GDGIPD LE DSDGDGIPDY+ED DGDGIPDYLD+D +   I                ++++  + + D L +D+DGDGIPD+LEKDSDGDGIPDY+ED DGDGIPDYL  D + DGI
Sbjct:  945 EDADGDGIPDNLEGDSDGDGIPDYEEDEDGDGIPDYLDEDADGDGIPDHLEGDSDGDGIPDYQEDADGDGIPDYLDEDADGDGIPDHLEKDSDGDGIPDYEEDADGDGIPDYLDDDDNNDGI 1066          

HSP 6 Score: 108.997 bits (271), Expect = 2.379e-25
Identity = 73/119 (61.34%), Postives = 82/119 (68.91%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGI------------PDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            LEDENG+GIPD LE D++GDGIPDY EDADGDGI            PDYLD+D     I   +       + K D DGDGIPD LE+D+DGDGIPDY EDEDGDGIPDYL+ D DGDGI
Sbjct:  609 LEDENGNGIPDALEGDANGDGIPDYLEDADGDGIKDYLEDSDNDGIPDYLDEDASIFTI---THFQQTKLSSKIDKDGDGIPDALEEDNDGDGIPDYLEDEDGDGIPDYLE-DQDGDGI 723          

HSP 7 Score: 107.457 bits (267), Expect = 1.082e-24
Identity = 68/115 (59.13%), Postives = 83/115 (72.17%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDAD--------GDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            L D++GDGIPD LE+DSDGDG PDY +D+D        GDGIPDY          +++   + V D L +D+DG+GIPDYLE DSDGDGIPDY+ED DGDGIPDYL +D+DGDGI
Sbjct:  810 LADQDGDGIPDALEQDSDGDGTPDYLQDSDDELEGDSDGDGIPDY----------QEDEDGDGVPDYLDEDADGNGIPDYLEGDSDGDGIPDYEEDADGDGIPDYLDEDADGDGI 914          

HSP 8 Score: 96.2857 bits (238), Expect = 7.614e-21
Identity = 71/163 (43.56%), Postives = 90/163 (55.21%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDS------------------------------------------DGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKN-------NVLDNLKQDSDGDGIPDY-------LEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            LED++GDGIP+YL+ D                                           D DG+P+Y EDAD DGIPD++DK T++  IK+  + N        + D L+QDSDGDG PDY       LE DSDGDGIPDY+EDEDGDG+PDYL +D+DG+GI
Sbjct:  715 LEDQDGDGIPNYLDADEEKPGGGGPTLSAEDEDDDKDTTDKIFEQDSNNNGIPDYLEDWDEDGVPNYLEDADNDGIPDFMDK-TDNRKIKRRFNFNLADQDGDGIPDALEQDSDGDGTPDYLQDSDDELEGDSDGDGIPDYQEDEDGDGVPDYLDEDADGNGI 876          

HSP 9 Score: 89.3521 bits (220), Expect = 1.899e-18
Identity = 61/115 (53.04%), Postives = 79/115 (68.70%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKK--------ESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
             +D +G+GIPD  E DSD DGIPDY ED+D DGIPD +D + +     K        +S  + ++D+L +D +G+GIPD LE D++GDGIPDY ED DGDGI DYL+ DSD DGI
Sbjct:  542 FKDSDGNGIPDGCEGDSDKDGIPDYLEDSDSDGIPDCIDDEPQPVEKDKPRKIARFVDSDGDGIMDHL-EDENGNGIPDALEGDANGDGIPDYLEDADGDGIKDYLE-DSDNDGI 654          

HSP 10 Score: 88.9669 bits (219), Expect = 3.209e-18
Identity = 67/142 (47.18%), Postives = 82/142 (57.75%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTEST--------------------NIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKE----------------DEDGDGIPDYLQKDSDGDGI 318
            ED +GDGIPDYLE D DGDGIPDY ED DGDGIP+YLD D E                       I ++ S NN + +  +D D DG+P+YLE D+D DGIPD+ +                D+DGDGIPD L++DSDGDG 
Sbjct:  692 EDNDGDGIPDYLE-DEDGDGIPDYLEDQDGDGIPNYLDADEEKPGGGGPTLSAEDEDDDKDTTDKIFEQDSNNNGIPDYLEDWDEDGVPNYLE-DADNDGIPDFMDKTDNRKIKRRFNFNLADQDGDGIPDALEQDSDGDGT 831          

HSP 11 Score: 84.3445 bits (207), Expect = 1.228e-16
Identity = 53/102 (51.96%), Postives = 68/102 (66.67%), Query Frame = -1
Query:   16 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDS 321
            LED++GDG+PD+LE D D DG+P++ ED D DGIP+YLDK       K    + +       DSD DGI D +E D D DGIP++ ED DGDGIP+Y+ KDS
Sbjct:  430 LEDDDGDGVPDFLE-DDDEDGVPNFLEDNDNDGIPNYLDKTPNKVVKKPVKLRTHARKLAVADSDNDGISDMME-DDDNDGIPNFLEDSDGDGIPNYMDKDS 529          

HSP 12 Score: 64.6994 bits (156), Expect = 9.927e-10
Identity = 59/129 (45.74%), Postives = 78/129 (60.47%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGI------------PDYLDKDTESTNIKK----------ESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            L DE+GD IPD LE+D D DG+ DY  D D DGI            P+ LD+D+    +KK          +  ++ V D L+ D D DGIPDYLE D DGDG+PD+ ED+D DG+P++L+ D+D DGI
Sbjct:  336 LLDEDGDDIPDLLEEDEDDDGVADYLRDKDKDGIRDYYEDDDGDGVPNMLDRDSSLFLLKKVIKWKMPKDMDRDRDGVPDFLQGDKDNDGIPDYLE-DDDGDGVPDFLEDDDEDGVPNFLE-DNDNDGI 462          

HSP 13 Score: 58.5362 bits (140), Expect = 1.310e-7
Identity = 48/80 (60.00%), Postives = 57/80 (71.25%), Query Frame = -1
Query:   40 KDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGI 279
            KDSDGDGIPDY EDADGDGIP+YLD D+ S + K+ S    ++  L  D DGD IPD LE+D D DG+ DY  D+D DGI
Sbjct:  293 KDSDGDGIPDYLEDADGDGIPNYLDDDSPSFS-KRFSYYMKLVKLL--DEDGDDIPDLLEEDEDDDGVADYLRDKDKDGI 369          
BLAST of SMED30013577 vs. Ensembl Nematostella
Match: EDO33541 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRY2])

HSP 1 Score: 46.2098 bits (108), Expect = 3.543e-7
Identity = 20/87 (22.99%), Postives = 36/87 (41.38%), Query Frame = -1
Query:   28 LEKDSDGDGIPDYKEDADGDGIPDYLDK-DTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYL 285
            +    DG G+P  +   DG G+P    + D +  +  +       +  ++   D  G+P    +  DG G+P  +   DG G+P   
Sbjct:   49 VRTQGDGQGVPGVRTKGDGQGVPGVCTQGDGQGVSGVRTQGDGQGVSGVRTKGDVQGVPGVCTQ-GDGQGVPGVRTQRDGQGVPGVC 134          

HSP 2 Score: 43.8986 bits (102), Expect = 2.267e-6
Identity = 25/97 (25.77%), Postives = 43/97 (44.33%), Query Frame = -1
Query:    1 LEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNV--LDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 285
            + K  DG G+P  +   DG G+P    +  +   +    +  +V  +  ++   DG G+P    K  DG G+P      DG G+   ++   DG G+
Sbjct:    1 MRKQRDGQGVPGVRTQRDGQGVPGVRTQR-DGQGVPGVCTLGDVQGVSGVRTQGDGQGVPGVRTK-GDGQGVPGVCTQGDGQGV-SGVRTQGDGQGV 94          
BLAST of SMED30013577 vs. Ensembl Nematostella
Match: EDO35541 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLA5])

HSP 1 Score: 43.5134 bits (101), Expect = 2.688e-6
Identity = 32/107 (29.91%), Postives = 49/107 (45.79%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            L    GDG+P  L   + GDG+P       GDG+P  L            +++ + + +    + GDG+P  L   + GDG+P       GDG+P  L   + GDG+
Sbjct:  185 LHTTRGDGMPSDLHT-TRGDGMPSNLHTTRGDGMPSNL-----------HTTRGDGMPSDLHTTRGDGMPSDLHT-TRGDGMPSNLHTTRGDGMPSDLHT-TRGDGM 277          
BLAST of SMED30013577 vs. Planmine SMEST
Match: SMESG000054821.1 (SMESG000054821.1)

HSP 1 Score: 196.823 bits (499), Expect = 1.342e-59
Identity = 106/107 (99.07%), Postives = 107/107 (100.00%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            +EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI
Sbjct: 1152 MEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 1258          

HSP 2 Score: 135.961 bits (341), Expect = 2.389e-38
Identity = 79/106 (74.53%), Postives = 86/106 (81.13%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            EDENGDGIPDYLEKDSDGDGIPD+KED+DGDGIPDYLDKDT+   I           N K+D+DGDGIPDYL+KDSDGDGIPDYKEDEDGDGIPDYL KD  G  +
Sbjct: 1288 EDENGDGIPDYLEKDSDGDGIPDFKEDSDGDGIPDYLDKDTDGDGIP----------NYKEDADGDGIPDYLDKDSDGDGIPDYKEDEDGDGIPDYLDKDYKGKSL 1383          

HSP 3 Score: 135.961 bits (341), Expect = 2.710e-38
Identity = 82/114 (71.93%), Postives = 92/114 (80.70%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKES-----SKNNVLDN---LKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            ED +GDGIPDYL+KDSDGDGIPDYKED DGDGIPDYLDKD +  ++ K       SK + + +     +DSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYL+ DSDGDGI
Sbjct: 1338 EDADGDGIPDYLDKDSDGDGIPDYKEDEDGDGIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGI 1451          

HSP 4 Score: 133.65 bits (335), Expect = 1.798e-37
Identity = 77/106 (72.64%), Postives = 88/106 (83.02%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            EDE+GDGIPDYLEKDSDGDGIPDY+ED +GDGIPDYL+KD++   I           + K+DSDGDGIPDYL+KD+DGDGIP+YKED DGDGIPDYL KDSDGDGI
Sbjct: 1263 EDEDGDGIPDYLEKDSDGDGIPDYQEDENGDGIPDYLEKDSDGDGIP----------DFKEDSDGDGIPDYLDKDTDGDGIPNYKEDADGDGIPDYLDKDSDGDGI 1358          

HSP 5 Score: 132.109 bits (331), Expect = 5.281e-37
Identity = 77/106 (72.64%), Postives = 86/106 (81.13%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            EDE+GDGIPDYL+ DSDGDGIPDYKED DGDGIPDYL+KD++   I           +  +D +GDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYL+ DSDGDGI
Sbjct: 1053 EDEDGDGIPDYLQNDSDGDGIPDYKEDEDGDGIPDYLEKDSDGDGIP----------DYMEDENGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGI 1148          

HSP 6 Score: 132.109 bits (331), Expect = 6.796e-37
Identity = 77/106 (72.64%), Postives = 86/106 (81.13%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            EDE+GDGIPDYLEKDSDGDGIPDY ED +GDGIPDYL+KD++   I           + K+D DGDGIPDYLE DSDGDGIPDY EDE+GDGIPDYL+KDSDGDGI
Sbjct: 1078 EDEDGDGIPDYLEKDSDGDGIPDYMEDENGDGIPDYLEKDSDGDGIP----------DYKEDEDGDGIPDYLENDSDGDGIPDYMEDENGDGIPDYLEKDSDGDGI 1173          

HSP 7 Score: 114.775 bits (286), Expect = 7.053e-31
Identity = 67/98 (68.37%), Postives = 77/98 (78.57%), Query Frame = -1
Query:   25 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQ 318
            +D +GDGIPDYLEKDSDGDGIPDYKED DGDGIPDYL+ D++   I           +  +D +GDGIPDYLEKDSDGDGIPDY+EDEDGDGIPDY +
Sbjct: 1406 KDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIP----------DYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHK 1493          

HSP 8 Score: 102.064 bits (253), Expect = 1.838e-26
Identity = 64/100 (64.00%), Postives = 71/100 (71.00%), Query Frame = -1
Query:    1 IPDYLEKDSDGDGIPDYKE-DADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 297
            + + L+KDSDGDGIPDY E D+DGDGIPDY                       K+D DGDGIPDYL+ DSDGDGIPDYKEDEDGDGIPDYL+KDSDGDGI
Sbjct: 1022 VAENLKKDSDGDGIPDYLEKDSDGDGIPDY-----------------------KEDEDGDGIPDYLQNDSDGDGIPDYKEDEDGDGIPDYLEKDSDGDGI 1098          

HSP 9 Score: 95.1301 bits (235), Expect = 5.400e-24
Identity = 55/68 (80.88%), Postives = 60/68 (88.24%), Query Frame = -1
Query:    1 DTESTNI-KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 201
            DTE TN   K+S ++ V +NLK+DSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQ DSDGDGI
Sbjct: 1006 DTEKTNKGIKDSKESLVAENLKKDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQNDSDGDGI 1073          

HSP 10 Score: 79.337 bits (194), Expect = 2.032e-18
Identity = 52/87 (59.77%), Postives = 60/87 (68.97%), Query Frame = -1
Query:   97 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDY----------LDKDTESTNIKKES--SKNNVLDNLKQDSDGDGIPDYLEK 321
            LEDENGDGIPDYLEKDSDGDGIPDY+ED DGDGIPDY            K T S +I K +   K+ V  +  +DSDGDGIPD ++K
Sbjct: 1455 LEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDK 1541          

HSP 11 Score: 58.5362 bits (140), Expect = 4.031e-11
Identity = 45/94 (47.87%), Postives = 60/94 (63.83%), Query Frame = -1
Query:   37 DENGDGIPDYLEKDSDGDGIPDYKEDADGD-GIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIP 315
            D NGDGIPD + KDSDGDGIPD+ +  D + GIPD  D +    N   +S  + + D++  D D DGIPD+ + D+DGDG  D   D+D DG+P
Sbjct:  337 DSNGDGIPDCIMKDSDGDGIPDHIDTDDDNDGIPDLKDPNHPLFNFFMDSDNDGIPDSMDNDDDNDGIPDHRDYDADGDGESDLFPDKDKDGVP 430          

HSP 12 Score: 53.5286 bits (127), Expect = 1.934e-9
Identity = 59/161 (36.65%), Postives = 74/161 (45.96%), Query Frame = -1
Query:    1 DENGDGIPDYLE-----------------KDSDGDGIPDYK-------------EDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPD----------YLEKDSDGD---------------GIPDYKE-DEDGDGIPDYLQKDSDGDGI 315
            D NGDGIPD  E                  DSD DG+PD               ED+DG+ IPD+LDK+ +  N  K   +  + D  +QD D DGIPD          + +K  D D               GIPD ++ D +GDGIPD + KDSDGDGI
Sbjct:  198 DSNGDGIPDCREDSSDNKLKLRFKSRQKRLDSDMDGVPDDSDIDDDQDGIPDILEDSDGNDIPDFLDKNRDVNN--KADFEAILADAARQDHDCDGIPDQYDPDDDNDGFFDKKQDSDRDGILNEYDDDDDNDGIPDSEDPDSNGDGIPDCIMKDSDGDGI 356          

HSP 13 Score: 47.7506 bits (112), Expect = 1.960e-7
Identity = 53/134 (39.55%), Postives = 70/134 (52.24%), Query Frame = -1
Query:    1 DENGDGIPDYLEKDSDGDGIPDYKEDADGD-GIPDYLDKDTESTNIKKESSKNNVL-----------DNL----KQDSDGDGIPDY------------LEKDSDGDG-IPDYKEDEDGDGIPDYLQKDSDGDGI 315
            D NGDGI D   +DSDGDGIPD+ +  D + GIPDYLD D+ +  + K + + + L           DN+      D D DG+PD               +DSD DG +    ED+D DGIPD    D++GDGI
Sbjct:  499 DSNGDGIIDCRPRDSDGDGIPDHLDPDDDNDGIPDYLDPDSMAFLLYKRTQQMDALPASQARHEANTDNVDLGDPGDKDCDGVPDDIDLDIDNDGKIDRTQDSDMDGELNHIDEDDDNDGIPDIEDPDANGDGI 632          

HSP 14 Score: 45.8246 bits (107), Expect = 1.161e-6
Identity = 51/154 (33.12%), Postives = 71/154 (46.10%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDY--------KEDADGDGIPD----------YLDKDTES------TNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIP-----------------------DYKEDEDGDGIPDYLQKDSDGDGI 321
            LED +G+ IPD+L+K+ D +   D+        ++D D DGIPD          + DK  +S           +   + + D+   DS+GDGIPD + KDSDGDGIP                       ++  D D DGIPD +  D D DGI
Sbjct:  251 LEDSDGNDIPDFLDKNRDVNNKADFEAILADAARQDHDCDGIPDQYDPDDDNDGFFDKKQDSDRDGILNEYDDDDDNDGIPDSEDPDSNGDGIPDCIMKDSDGDGIPDHIDTDDDNDGIPDLKDPNHPLFNFFMDSDNDGIPDSMDNDDDNDGI 404          
BLAST of SMED30013577 vs. Planmine SMEST
Match: SMESG000054821.1 (SMESG000054821.1)

HSP 1 Score: 196.823 bits (499), Expect = 1.479e-59
Identity = 106/107 (99.07%), Postives = 107/107 (100.00%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 321
            +EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI
Sbjct: 1170 MEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 1276          

HSP 2 Score: 136.346 bits (342), Expect = 2.026e-38
Identity = 79/106 (74.53%), Postives = 86/106 (81.13%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            EDENGDGIPDYLEKDSDGDGIPD+KED+DGDGIPDYLDKDT+   I           N K+D+DGDGIPDYL+KDSDGDGIPDYKEDEDGDGIPDYL KD  G  +
Sbjct: 1306 EDENGDGIPDYLEKDSDGDGIPDFKEDSDGDGIPDYLDKDTDGDGIP----------NYKEDADGDGIPDYLDKDSDGDGIPDYKEDEDGDGIPDYLDKDYKGKSL 1401          

HSP 3 Score: 135.961 bits (341), Expect = 2.344e-38
Identity = 82/114 (71.93%), Postives = 92/114 (80.70%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKES-----SKNNVLDN---LKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            ED +GDGIPDYL+KDSDGDGIPDYKED DGDGIPDYLDKD +  ++ K       SK + + +     +DSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYL+ DSDGDGI
Sbjct: 1356 EDADGDGIPDYLDKDSDGDGIPDYKEDEDGDGIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGI 1469          

HSP 4 Score: 133.65 bits (335), Expect = 1.555e-37
Identity = 77/106 (72.64%), Postives = 88/106 (83.02%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            EDE+GDGIPDYLEKDSDGDGIPDY+ED +GDGIPDYL+KD++   I           + K+DSDGDGIPDYL+KD+DGDGIP+YKED DGDGIPDYL KDSDGDGI
Sbjct: 1281 EDEDGDGIPDYLEKDSDGDGIPDYQEDENGDGIPDYLEKDSDGDGIP----------DFKEDSDGDGIPDYLDKDTDGDGIPNYKEDADGDGIPDYLDKDSDGDGI 1376          

HSP 5 Score: 132.494 bits (332), Expect = 4.611e-37
Identity = 77/106 (72.64%), Postives = 86/106 (81.13%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            EDE+GDGIPDYL+ DSDGDGIPDYKED DGDGIPDYL+KD++   I           +  +D +GDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYL+ DSDGDGI
Sbjct: 1071 EDEDGDGIPDYLQNDSDGDGIPDYKEDEDGDGIPDYLEKDSDGDGIP----------DYMEDENGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGI 1166          

HSP 6 Score: 132.109 bits (331), Expect = 5.878e-37
Identity = 77/106 (72.64%), Postives = 86/106 (81.13%), Query Frame = -1
Query:    1 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 318
            EDE+GDGIPDYLEKDSDGDGIPDY ED +GDGIPDYL+KD++   I           + K+D DGDGIPDYLE DSDGDGIPDY EDE+GDGIPDYL+KDSDGDGI
Sbjct: 1096 EDEDGDGIPDYLEKDSDGDGIPDYMEDENGDGIPDYLEKDSDGDGIP----------DYKEDEDGDGIPDYLENDSDGDGIPDYMEDENGDGIPDYLEKDSDGDGI 1191          

HSP 7 Score: 114.775 bits (286), Expect = 6.341e-31
Identity = 67/98 (68.37%), Postives = 77/98 (78.57%), Query Frame = -1
Query:   25 EDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQ 318
            +D +GDGIPDYLEKDSDGDGIPDYKED DGDGIPDYL+ D++   I           +  +D +GDGIPDYLEKDSDGDGIPDY+EDEDGDGIPDY +
Sbjct: 1424 KDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIP----------DYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHK 1511          

HSP 8 Score: 102.449 bits (254), Expect = 1.574e-26
Identity = 64/100 (64.00%), Postives = 71/100 (71.00%), Query Frame = -1
Query:    1 IPDYLEKDSDGDGIPDYKE-DADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 297
            + + L+KDSDGDGIPDY E D+DGDGIPDY                       K+D DGDGIPDYL+ DSDGDGIPDYKEDEDGDGIPDYL+KDSDGDGI
Sbjct: 1040 VAENLKKDSDGDGIPDYLEKDSDGDGIPDY-----------------------KEDEDGDGIPDYLQNDSDGDGIPDYKEDEDGDGIPDYLEKDSDGDGI 1116          

HSP 9 Score: 95.1301 bits (235), Expect = 4.902e-24
Identity = 55/68 (80.88%), Postives = 60/68 (88.24%), Query Frame = -1
Query:    1 DTESTNI-KKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGI 201
            DTE TN   K+S ++ V +NLK+DSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQ DSDGDGI
Sbjct: 1024 DTEKTNKGIKDSKESLVAENLKKDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQNDSDGDGI 1091          

HSP 10 Score: 79.337 bits (194), Expect = 1.936e-18
Identity = 52/87 (59.77%), Postives = 60/87 (68.97%), Query Frame = -1
Query:   97 LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDY----------LDKDTESTNIKKES--SKNNVLDNLKQDSDGDGIPDYLEK 321
            LEDENGDGIPDYLEKDSDGDGIPDY+ED DGDGIPDY            K T S +I K +   K+ V  +  +DSDGDGIPD ++K
Sbjct: 1473 LEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDK 1559          

HSP 11 Score: 58.5362 bits (140), Expect = 3.730e-11
Identity = 45/94 (47.87%), Postives = 60/94 (63.83%), Query Frame = -1
Query:   37 DENGDGIPDYLEKDSDGDGIPDYKEDADGD-GIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIP 315
            D NGDGIPD + KDSDGDGIPD+ +  D + GIPD  D +    N   +S  + + D++  D D DGIPD+ + D+DGDG  D   D+D DG+P
Sbjct:  355 DSNGDGIPDCIMKDSDGDGIPDHIDTDDDNDGIPDLKDPNHPLFNFFMDSDNDGIPDSMDNDDDNDGIPDHRDYDADGDGESDLFPDKDKDGVP 448          

HSP 12 Score: 53.9138 bits (128), Expect = 1.624e-9
Identity = 59/161 (36.65%), Postives = 74/161 (45.96%), Query Frame = -1
Query:    1 DENGDGIPDYLE-----------------KDSDGDGIPDYK-------------EDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDSDGDGIPD----------YLEKDSDGD---------------GIPDYKE-DEDGDGIPDYLQKDSDGDGI 315
            D NGDGIPD  E                  DSD DG+PD               ED+DG+ IPD+LDK+ +  N  K   +  + D  +QD D DGIPD          + +K  D D               GIPD ++ D +GDGIPD + KDSDGDGI
Sbjct:  216 DSNGDGIPDCREDSSDNKLKLRFKSRQKRLDSDMDGVPDDSDIDDDQDGIPDILEDSDGNDIPDFLDKNRDVNN--KADFEAILADAARQDHDCDGIPDQYDPDDDNDGFFDKKQDSDRDGILNEYDDDDDNDGIPDSEDPDSNGDGIPDCIMKDSDGDGI 374          

HSP 13 Score: 48.1358 bits (113), Expect = 1.762e-7
Identity = 53/134 (39.55%), Postives = 70/134 (52.24%), Query Frame = -1
Query:    1 DENGDGIPDYLEKDSDGDGIPDYKEDADGD-GIPDYLDKDTESTNIKKESSKNNVL-----------DNL----KQDSDGDGIPDY------------LEKDSDGDG-IPDYKEDEDGDGIPDYLQKDSDGDGI 315
            D NGDGI D   +DSDGDGIPD+ +  D + GIPDYLD D+ +  + K + + + L           DN+      D D DG+PD               +DSD DG +    ED+D DGIPD    D++GDGI
Sbjct:  517 DSNGDGIIDCRPRDSDGDGIPDHLDPDDDNDGIPDYLDPDSMAFLLYKRTQQMDALPASQARHEANTDNVDLGDPGDKDCDGVPDDIDLDIDNDGKIDRTQDSDMDGELNHIDEDDDNDGIPDIEDPDANGDGI 650          

HSP 14 Score: 45.8246 bits (107), Expect = 1.004e-6
Identity = 51/154 (33.12%), Postives = 71/154 (46.10%), Query Frame = -1
Query:    1 LEDENGDGIPDYLEKDSDGDGIPDY--------KEDADGDGIPD----------YLDKDTES------TNIKKESSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIP-----------------------DYKEDEDGDGIPDYLQKDSDGDGI 321
            LED +G+ IPD+L+K+ D +   D+        ++D D DGIPD          + DK  +S           +   + + D+   DS+GDGIPD + KDSDGDGIP                       ++  D D DGIPD +  D D DGI
Sbjct:  269 LEDSDGNDIPDFLDKNRDVNNKADFEAILADAARQDHDCDGIPDQYDPDDDNDGFFDKKQDSDRDGILNEYDDDDDNDGIPDSEDPDSNGDGIPDCIMKDSDGDGIPDHIDTDDDNDGIPDLKDPNHPLFNFFMDSDNDGIPDSMDNDDDNDGI 422          
The following BLAST results are available for this feature:
BLAST of SMED30013577 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013577 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013577 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013577 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013577 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013577 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013577 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013577 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0X3PD461.811e-3160.68Thrombospondin-3b (Fragment) OS=Schistocephalus so... [more]
A0A183T0D35.627e-3162.30Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A3P7CSP75.682e-3162.30Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A504YRT13.859e-3071.43Septin-10 OS=Fasciola gigantica OX=46835 GN=FGIG_0... [more]
A0A3P7MJ556.392e-3061.48Uncharacterized protein (Fragment) OS=Dibothriocep... [more]
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BLAST of SMED30013577 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013577 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013577 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013577 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO335413.543e-722.99Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO355412.688e-629.91Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30013577 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013577 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000054821.11.342e-5999.07SMESG000054821.1[more]
SMESG000054821.11.479e-5999.07SMESG000054821.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013577 ID=SMED30013577|Name=SMED30013577|organism=Schmidtea mediterranea sexual|type=transcript|length=321bp
GATTCCATCGCCATCTGAGTCTTTTTGTAAATAATCCGGAATGCCATCAC
CATCTTCATCTTCTTTGTAGTCCGGAATTCCATCTCCATCGGAATCTTTC
TCCAAGTAATCTGGTATTCCGTCACCGTCAGAATCTTGTTTTAAATTATC
TAATACATTATTTTTACTTGATTCTTTTTTAATATTTGTGCTTTCTGTAT
CTTTGTCTAAATAATCAGGAATTCCGTCTCCATCAGCATCTTCTTTGTAA
TCTGGTATTCCATCGCCGTCAGAATCCTTCTCCAAATAATCTGGAATTCC
ATCTCCATTCTCATCTTCCAA
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protein sequence of SMED30013577-orf-1

>SMED30013577-orf-1 ID=SMED30013577-orf-1|Name=SMED30013577-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp
LEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKE
SSKNNVLDNLKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQK
DSDGDGI
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000428musculature system
PLANA:0000478pharynx musculature
Vocabulary: INTERPRO
TermDefinition
IPR003367Thrombospondin_3-like_rpt
IPR028974TSP_type-3_rpt
Vocabulary: biological process
TermDefinition
GO:0007155cell adhesion
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003367Thrombospondin, type 3-like repeatPFAMPF02412TSP_3coord: 1..24
e-value: 0.0028
score: 17.3
coord: 61..84
e-value: 8.2E-4
score: 19.0
IPR028974TSP type-3 repeatGENE3DG3DSA:4.10.1080.10coord: 24..87
e-value: 4.2E-5
score: 25.5
IPR028974TSP type-3 repeatSUPERFAMILYSSF103647TSP type-3 repeatcoord: 2..85
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 91..106
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 9..47
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 63..81
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..106
NoneNo IPR availablePANTHERPTHR10199:SF100THROMBOSPONDIN, ISOFORM Acoord: 2..106
NoneNo IPR availablePANTHERPTHR10199THROMBOSPONDINcoord: 2..106