SMED30013574

Overview
NameSMED30013574
Smed IDSMED30013574
Length (bp)481
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30013574 (SMED30013574) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30013574 (SMED30013574) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30013574 (SMED30013574) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30013574 (SMED30013574) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013574

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 26

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30013574h1SMcG0018855 SmedASXL_004582SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pigment cellSMED30013574h1SMcG0018855 SmedASXL_004582SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30013574h1SMcG0018854 Contig14068uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013574h1SMcG0018854 Contig14068newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013574h1SMcG0018855 Contig10526uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013574h1SMcG0018855 Contig10526newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013574h1SMcG0018856 Contig10526uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013574h1SMcG0018856 Contig10526newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013574h1SMcG0018853 Contig14068uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013574h1SMcG0018853 Contig14068newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013574h1SMcG0018855 Contig14068uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013574h1SMcG0018855 Contig14068newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013574h1SMcG0018856 Contig14068uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013574h1SMcG0018856 Contig14068newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30013574h1SMcG0018856 dd_Smed_v4_10746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30013574h1SMcG0018856 dd_Smed_v4_10746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30013574h1SMcG0018855 dd_Smed_v4_10746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30013574h1SMcG0018855 dd_Smed_v4_10746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30013574h1SMcG0018855 dd_Smed_v4_7381_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30013574h1SMcG0018855 dd_Smed_v6_7381_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30013574h1SMcG0018855 dd_Smed_v6_21309_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30013574h1SMcG0018854 dd_Smed_v6_21309_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30013574h1SMcG0018856 dd_Smed_v6_10746_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30013574h1SMcG0018855 dd_Smed_v6_10746_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30013574h1SMcG0018854 OX_Smed_1.0.05553ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30013574h1SMcG0018855 OX_Smed_1.0.05553ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30013574 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000191:148490..153508 +2359
v31.000191:139101..147769 +2327
v31.000191:147843..149063 +2291
v31.000191:153582..154015 +768
v31.000191:138572..139027 +733
Homology
[Close]
BLAST of SMED30013574 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 228.024 bits (580), Expect = 1.705e-69
Identity = 158/159 (99.37%), Postives = 158/159 (99.37%), Query Frame = 3 
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYEXXXXXXXXXXXXXXXXXXKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 479
            EISFVIKLTPKSNTEVNIVNLEINVN NSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE
Sbjct:  175 EISFVIKLTPKSNTEVNIVNLEINVNINSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 333

HSP 2 Score: 178.333 bits (451), Expect = 3.188e-52
Identity = 114/119 (95.80%), Postives = 116/119 (97.48%), Query Frame = 3 
Query:  123 EIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYEXXXXXXXXXXXXXXXXXXKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 479
            +I+ P  KKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE
Sbjct:   50 QILSP--KKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 166

HSP 3 Score: 109.768 bits (273), Expect = 3.392e-28
Identity = 71/96 (73.96%), Postives = 84/96 (87.50%), Query Frame = 3 
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 290
            EISFVIKLTPKSNT+VNIVNLEINVN NSIVT+TFGKKS+EI++ +YK  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:  342 EISFVIKLTPKSNTKVNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 437
[Close]
BLAST of SMED30013574 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 228.024 bits (580), Expect = 1.705e-69
Identity = 158/159 (99.37%), Postives = 158/159 (99.37%), Query Frame = 3 
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYEXXXXXXXXXXXXXXXXXXKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 479
            EISFVIKLTPKSNTEVNIVNLEINVN NSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE
Sbjct:  177 EISFVIKLTPKSNTEVNIVNLEINVNINSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 335

HSP 2 Score: 181.8 bits (460), Expect = 2.098e-53
Identity = 114/119 (95.80%), Postives = 116/119 (97.48%), Query Frame = 3 
Query:  123 EIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYEXXXXXXXXXXXXXXXXXXKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 479
            +I+ P  KKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE
Sbjct:   50 QILSPVRKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 168

HSP 3 Score: 109.768 bits (273), Expect = 3.392e-28
Identity = 71/96 (73.96%), Postives = 84/96 (87.50%), Query Frame = 3 
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 290
            EISFVIKLTPKSNT+VNIVNLEINVN NSIVT+TFGKKS+EI++ +YK  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:  344 EISFVIKLTPKSNTKVNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 439
[Close]
BLAST of SMED30013574 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 228.024 bits (580), Expect = 1.705e-69
Identity = 158/159 (99.37%), Postives = 158/159 (99.37%), Query Frame = 3 
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYEXXXXXXXXXXXXXXXXXXKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 479
            EISFVIKLTPKSNTEVNIVNLEINVN NSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE
Sbjct:  177 EISFVIKLTPKSNTEVNIVNLEINVNINSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 335

HSP 2 Score: 181.8 bits (460), Expect = 2.098e-53
Identity = 114/119 (95.80%), Postives = 116/119 (97.48%), Query Frame = 3 
Query:  123 EIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYEXXXXXXXXXXXXXXXXXXKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 479
            +I+ P  KKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE
Sbjct:   50 QILSPVRKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 168

HSP 3 Score: 109.768 bits (273), Expect = 3.392e-28
Identity = 71/96 (73.96%), Postives = 84/96 (87.50%), Query Frame = 3 
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 290
            EISFVIKLTPKSNT+VNIVNLEINVN NSIVT+TFGKKS+EI++ +YK  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:  344 EISFVIKLTPKSNTKVNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 439
[Close]
BLAST of SMED30013574 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 227.639 bits (579), Expect = 1.755e-69
Identity = 158/159 (99.37%), Postives = 158/159 (99.37%), Query Frame = 3 
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYEXXXXXXXXXXXXXXXXXXKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 479
            EISFVIKLTPKSNTEVNIVNLEINVN NSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE
Sbjct:  175 EISFVIKLTPKSNTEVNIVNLEINVNINSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 333

HSP 2 Score: 178.333 bits (451), Expect = 3.188e-52
Identity = 114/119 (95.80%), Postives = 116/119 (97.48%), Query Frame = 3 
Query:  123 EIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYEXXXXXXXXXXXXXXXXXXKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 479
            +I+ P  KKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE
Sbjct:   50 QILSP--KKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 166

HSP 3 Score: 109.383 bits (272), Expect = 3.629e-28
Identity = 71/96 (73.96%), Postives = 84/96 (87.50%), Query Frame = 3 
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 290
            EISFVIKLTPKSNT+VNIVNLEINVN NSIVT+TFGKKS+EI++ +YK  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:  342 EISFVIKLTPKSNTKVNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 437
[Close]
BLAST of SMED30013574 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 227.639 bits (579), Expect = 1.755e-69
Identity = 158/159 (99.37%), Postives = 158/159 (99.37%), Query Frame = 3 
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYEXXXXXXXXXXXXXXXXXXKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 479
            EISFVIKLTPKSNTEVNIVNLEINVN NSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE
Sbjct:  175 EISFVIKLTPKSNTEVNIVNLEINVNINSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 333

HSP 2 Score: 178.333 bits (451), Expect = 3.188e-52
Identity = 114/119 (95.80%), Postives = 116/119 (97.48%), Query Frame = 3 
Query:  123 EIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYEXXXXXXXXXXXXXXXXXXKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 479
            +I+ P  KKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE
Sbjct:   50 QILSP--KKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTTTTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVTDVEKILTSE 166

HSP 3 Score: 109.383 bits (272), Expect = 3.594e-28
Identity = 71/96 (73.96%), Postives = 84/96 (87.50%), Query Frame = 3 
Query:    3 EISFVIKLTPKSXXXXXXXXXXXXXXXXSIVTVTFGKKSEEIIQPEYKKTGTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYE 290
            EISFVIKLTPKSNT+VNIVNLEINVN NSIVT+TFGKKS+EI++ +YK  G+E  +F F++TDKNGEQFEY+KVS+ T  G  LTVKNL+VKVCYE
Sbjct:  342 EISFVIKLTPKSNTKVNIVNLEINVNINSIVTITFGKKSDEIVESQYKSRGSEKFEFNFDQTDKNGEQFEYMKVSIRTNVGYTLTVKNLIVKVCYE 437
The following BLAST results are available for this feature:
BLAST of SMED30013574 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013574 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013574 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013574 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013574 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013574 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013574 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013574 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013574 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013574 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013574 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013574 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013574 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013574 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000025545.11.705e-6999.37SMESG000025545.1[more]
SMESG000025545.11.705e-6999.37SMESG000025545.1[more]
SMESG000025545.11.705e-6999.37SMESG000025545.1[more]
SMESG000025545.11.755e-6999.37SMESG000025545.1[more]
SMESG000025545.11.755e-6999.37SMESG000025545.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013574 ID=SMED30013574|Name=SMED30013574|organism=Schmidtea mediterranea sexual|type=transcript|length=481bp
ATGAAATCAGCTTTGTTATCAAATTGACACCGAAATCAAACACAGAAGTG
AACATTGTCAACCTAGAAATCAATGTCAACACAAACTCAATAGTGACAGT
TACATTCGGAAAGAAGAGTGAGGAGATAATTCAACCAGAATATAAGAAGA
CTGGCACTGAAACTATTAAATTTTACTTCAATAAAACTGACAAAAACGGA
GAACAATTCGAATATGTGAAAGTATCGGTCATTACAACTGATGGAAAGCC
TTTGACAGTGAAGAATCTGTTAGTGAAAGTTTGTTATGAAAGCGTAACGA
CAACAACTCAAGTGACAACTTCATCAACTGCATCAACATCAACGAAACCC
GTAATCACTACTCCAGAAGTCTGTGCAAAGGAAGTTGTAACTTTGACAAA
GAACGAAAAAATGAAAGTAGAAATACAAAATGTGACAAACGGAAATGTGA
CAGATGTGGAAAAGATACTGACAAGTGAAAA
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protein sequence of SMED30013574-orf-1

>SMED30013574-orf-1 ID=SMED30013574-orf-1|Name=SMED30013574-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=159bp
EISFVIKLTPKSNTEVNIVNLEINVNTNSIVTVTFGKKSEEIIQPEYKKT
GTETIKFYFNKTDKNGEQFEYVKVSVITTDGKPLTVKNLLVKVCYESVTT
TTQVTTSSTASTSTKPVITTPEVCAKEVVTLTKNEKMKVEIQNVTNGNVT
DVEKILTSE
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000420parapharyngeal region
PLANA:0002075pigment cell