DNA-directed RNA polymerase III subunit RPC8

Overview


Name	DNA-directed RNA polymerase III subunit RPC8
Smed ID	SMED30013558
Length (bp)	1864

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of DNA-directed RNA polymerase III subunit RPC8 (SMED30013558) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA-directed RNA polymerase III subunit RPC8 (SMED30013558) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA-directed RNA polymerase III subunit RPC8 (SMED30013558) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30013558

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
X1 cell	SMED30013558	SMESG000019826.1	SmedASXL_019939	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015

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Homology

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Human
Match: POLR3H (RNA polymerase III subunit H [Source:HGNC Symbol;Acc:HGNC:30349])

HSP 1 Score: 145.591 bits (366), Expect = 1.426e-39
Identity = 76/187 (40.64%), Postives = 111/187 (59.36%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQE--RSTAPFTVV 571
            MF +V + DTVRI P  F+      I +ELN+KL+NKV+ N GL ICL+DI    D  ++P DGA +  V FR V+F PF++EI+ G +  CSPEG+ VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +D  ++I F+V +  + DT P   S  +     +E  +  AP+T+V
Sbjct:    1 MFVLVEMVDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTSPTGPSSADATTSSEELPKKEAPYTLV 187

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Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQE--RSTAPFTVV 571
            MF +V + DTVRI P  F+      I +ELN+KL+NKV+ N GL ICL+DI    D  ++P DGA +  V FR V+F PF++EI+ G +  CSPEG+ VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +D  ++I F+V +  + DT P   S  +     +E  +  AP+T+V
Sbjct:    1 MFVLVEMVDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTSPTGPSSADATTSSEELPKKEAPYTLV 187

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Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQE--RSTAPFTVV 571
            MF +V + DTVRI P  F+      I +ELN+KL+NKV+ N GL ICL+DI    D  ++P DGA +  V FR V+F PF++EI+ G +  CSPEG+ VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +D  ++I F+V +  + DT P   S  +     +E  +  AP+T+V
Sbjct:    1 MFVLVEMVDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTSPTGPSSADATTSSEELPKKEAPYTLV 187

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Human
Match: POLR3H (RNA polymerase III subunit H [Source:HGNC Symbol;Acc:HGNC:30349])

HSP 1 Score: 98.2117 bits (243), Expect = 1.103e-23
Identity = 47/98 (47.96%), Postives = 64/98 (65.31%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQ 322
            MF +V + DTVRI P  F+      I +ELN+KL+NKV+ N GL ICL+DI    D  ++P DGA +  V FR V+F PF++EI+ G +  CSPEG+ 
Sbjct:    1 MFVLVEMVDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVH 98

[Close]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Human
Match: POLR3H (RNA polymerase III subunit H [Source:HGNC Symbol;Acc:HGNC:30349])

HSP 1 Score: 94.7449 bits (234), Expect = 7.061e-22
Identity = 61/187 (32.62%), Postives = 91/187 (48.66%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQE--RSTAPFTVV 571
            MF +V + DTVRI P  F+      I +ELN+KL+NKV+ N GL ICL+DI    D  ++P DGA +                              +VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +D  ++I F+V +  + DT P   S  +     +E  +  AP+T+V
Sbjct:    1 MFVLVEMVDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDITKLEDAYVFPGDGASHT-----------------------------KVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTSPTGPSSADATTSSEELPKKEAPYTLV 158

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Celegans
Match: rpc-25 (RNA Polymerase, Class III (C) [Source:UniProtKB/TrEMBL;Acc:Q23645])

HSP 1 Score: 111.694 bits (278), Expect = 1.432e-27
Identity = 58/164 (35.37%), Postives = 94/164 (57.32%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVW--TIENGE----ITIDVQDKINFKVTEYIWQDTFP 502
            MF +  + DTV I+P          I++ LN +L+NK++ + GL IC++DI + GD  I P +G     V FR ++F+PF++E+I   V   S +GL ++I+FF+DI +P   +P+   F+ E   W W    E+GE    + +D    + F+VTE I++D  P
Sbjct:    1 MFILSLLHDTVAIQPHQLSSDQQIVIKKRLNERLANKIIPDLGLCICVYDINEIGDSYILPGEGDCRARVKFRMIVFRPFVDEVIEAKVIGSSRQGLCLTIQFFEDIFVPAEKLPEPHVFEEEGQVWYWEYAQEDGEPPAKLYMDPGKIVRFRVTEIIFKDLKP 164

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Fly
Match: CG7339 (gene:FBgn0036188 transcript:FBtr0331530)

HSP 1 Score: 141.354 bits (355), Expect = 6.396e-38
Identity = 72/175 (41.14%), Postives = 103/175 (58.86%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVW--TIENG---EITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQ 538
            MF +  +KD VRI P  F L  V  +  E++RKL+NKVLLN GL I L DI+   D  I P DGA +  V FRYV+F+P +  +I G +  CS EG+ V++ FFDDI+IP A +     F   + +WVW   +E+G   ++ +DV + I F+V+  I+++T PI     E    Q
Sbjct:    1 MFVLAELKDNVRIAPDQFHLKLVDAVRDEIDRKLANKVLLNVGLCIALKDIVSLKDSIILPGDGASHTEVLFRYVVFRPMVGTVITGKIRNCSREGVHVTLGFFDDILIPHAALQHPSRFDEAEQAWVWEYPLEDGAKHDLFMDVGEPIKFRVSREIFEETSPIGPPKTEAQTQQ 175

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Fly
Match: CG7339 (gene:FBgn0036188 transcript:FBtr0076107)

HSP 1 Score: 141.354 bits (355), Expect = 6.396e-38
Identity = 72/175 (41.14%), Postives = 103/175 (58.86%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVW--TIENG---EITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQ 538
            MF +  +KD VRI P  F L  V  +  E++RKL+NKVLLN GL I L DI+   D  I P DGA +  V FRYV+F+P +  +I G +  CS EG+ V++ FFDDI+IP A +     F   + +WVW   +E+G   ++ +DV + I F+V+  I+++T PI     E    Q
Sbjct:    1 MFVLAELKDNVRIAPDQFHLKLVDAVRDEIDRKLANKVLLNVGLCIALKDIVSLKDSIILPGDGASHTEVLFRYVVFRPMVGTVITGKIRNCSREGVHVTLGFFDDILIPHAALQHPSRFDEAEQAWVWEYPLEDGAKHDLFMDVGEPIKFRVSREIFEETSPIGPPKTEAQTQQ 175

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Fly
Match: Rpb7 (gene:FBgn0051155 transcript:FBtr0083137)

HSP 1 Score: 50.8322 bits (120), Expect = 4.621e-7
Identity = 32/148 (21.62%), Postives = 66/148 (44.59%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKV 472
            MF  ++++  + + PR+F    ++ ++Q+L  ++        G  I +  I   G   I P  G     V ++ ++F+PF  E++   V + +  G+   I       I   +IP   +F    N   +  ++ ++ I  +DKI  K+
Sbjct:    1 MFYHISLEQEILLHPRYFGPQLLETVKQKLYSEVEGTCTGKYGFVIAVTTIDQIGSGVIQPGQGFVVYPVKYKAIVFRPFKGEVLDAVVKQINKVGMFAEIGPL-SCFISHHSIPADMQFCPNGNPPCYKSKDEDVVISGEDKIRLKI 147

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Zebrafish
Match: polr3h (polymerase (RNA) III (DNA directed) polypeptide H [Source:ZFIN;Acc:ZDB-GENE-040718-179])

HSP 1 Score: 149.058 bits (375), Expect = 6.169e-41
Identity = 74/169 (43.79%), Postives = 107/169 (63.31%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEIT----IDVQDKINFKVTEYIWQDTFPISNSVDE 523
            MF +V ++DTVRI P  F+    + + +ELN+KL+NKV+ N GL ICL+DI    D  I+P DGA +  V FRYV+F PF++EI+ G +  CS EG+ VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E T    +D  ++I F+VT+ ++ DT P   S D+
Sbjct:    1 MFVLVEMRDTVRIPPWSFQRPLNEAVAEELNKKLANKVVYNVGLCICLYDITKLEDSYIFPGDGASHTKVHFRYVVFHPFLDEILLGKIKGCSAEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETDEGTHDLYMDQGEEIRFRVTDEVFVDTSPSGPSADK 169

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Zebrafish
Match: polr2gl (polymerase (RNA) II (DNA directed) polypeptide G-like [Source:NCBI gene;Acc:324088])

HSP 1 Score: 53.9138 bits (128), Expect = 5.719e-8
Identity = 38/167 (22.75%), Postives = 73/167 (43.71%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKV--TEYIWQDTFPISNSVDE 523
            MF  ++++  + + PR+F    +  ++Q+L  ++        G  I +  I + G   I P  G     V ++ ++F+PF  E++   V + +  GL   I       I + +IP   EF    N   +   + ++ I   D+I  K+  T     D F I + +D+
Sbjct:    1 MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPM-SCFISRHSIPSEMEFDPNSNPPCYKTVDEDVVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDD 166

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Xenopus
Match: myadml2 (myeloid-associated differentiation marker-like 2 [Source:Xenbase;Acc:XB-GENE-967754])

HSP 1 Score: 147.902 bits (372), Expect = 3.385e-40
Identity = 82/201 (40.80%), Postives = 118/201 (58.71%), Query Frame = 2

Query:    2 LYIFKLYIL--MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVDENNRV-----QQERSTAPFTVV 571
            LY+F   +L  MF +V + DTVR  P  F+      I +ELN+KL+NKV+ N GL ICL+DI    D  I+P DGA +  V FRYV+F+PF++EI+ G +  CSPEG+ VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +DV + I F++ +  + DT P   S  E +       +  R  AP+T+V
Sbjct:   10 LYLFPRLLLAKMFVLVEMVDTVRTNPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDITKMDDSYIFPGDGASHTKVQFRYVVFRPFLDEILMGKIKGCSPEGVHVSLGFFDDIVIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDVGEDIRFRMVDETFIDTSPTGPSSAEASSSTAVAEETPRKEAPYTLV 210

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Xenopus
Match: myadml2 (myeloid-associated differentiation marker-like 2 [Source:Xenbase;Acc:XB-GENE-967754])

HSP 1 Score: 142.895 bits (359), Expect = 8.780e-39
Identity = 71/164 (43.29%), Postives = 101/164 (61.59%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPIS 508
            MF +V + DTVR  P  F+      I +ELN+KL+NKV+ N GL ICL+DI    D  I+P DGA +  V FRYV+F+PF++EI+ G +  CSPEG+ VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +DV + I F++ +  + DT P  
Sbjct:    1 MFVLVEMVDTVRTNPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDITKMDDSYIFPGDGASHTKVQFRYVVFRPFLDEILMGKIKGCSPEGVHVSLGFFDDIVIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDVGEDIRFRMVDETFIDTSPTG 164

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Xenopus
Match: polr2g (polymerase (RNA) II (DNA directed) polypeptide G [Source:NCBI gene;Acc:549819])

HSP 1 Score: 53.9138 bits (128), Expect = 8.149e-8
Identity = 38/167 (22.75%), Postives = 73/167 (43.71%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKV--TEYIWQDTFPISNSVDE 523
            MF  ++++  + + PR+F    +  ++Q+L  ++        G  I +  I + G   I P  G     V ++ ++F+PF  E++   V + +  GL   I       I + +IP   EF    N   +   + ++ I   D+I  K+  T     D F I + +D+
Sbjct:    1 MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPM-SCFISRHSIPSEMEFDPNSNPPCYKTVDEDVVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDD 166

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Xenopus
Match: polr2g (polymerase (RNA) II (DNA directed) polypeptide G [Source:NCBI gene;Acc:549819])

HSP 1 Score: 49.6766 bits (117), Expect = 2.075e-6
Identity = 31/144 (21.53%), Postives = 63/144 (43.75%), Query Frame = 2

Query:   41 VNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKV 472
            ++++  + + PR+F    +  ++Q+L  ++        G  I +  I + G   I P  G     V ++ ++F+PF  E++   V + +  GL   I       I + +IP   EF    N   +   + ++ I   D+I  K+
Sbjct:    4 ISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPM-SCFISRHSIPSEMEFDPNSNPPCYKTVDEDVVIQQDDEIRLKI 146

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Mouse
Match: Polr3h (polymerase (RNA) III (DNA directed) polypeptide H [Source:MGI Symbol;Acc:MGI:1926179])

HSP 1 Score: 142.51 bits (358), Expect = 1.117e-38
Identity = 77/187 (41.18%), Postives = 111/187 (59.36%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQ--ERSTAPFTVV 571
            MF +V + DTVRI P  F+      I +ELN+KL+NKV+ N GL ICL+DI    D  ++P DGA +  V FRYV+F PF++EI+ G +  CSPEG+ VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +D  ++I F+V +  + DT P   S  E     +   +  AP+T+V
Sbjct:    1 MFVLVEMVDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRYVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTSPTGPSSAEAASSSEELPKKEAPYTLV 187

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Mouse
Match: Polr3h (polymerase (RNA) III (DNA directed) polypeptide H [Source:MGI Symbol;Acc:MGI:1926179])

HSP 1 Score: 88.5817 bits (218), Expect = 6.913e-20
Identity = 61/187 (32.62%), Postives = 90/187 (48.13%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQ--ERSTAPFTVV 571
            MF +V + DTVRI P  F+      I +ELN+KL+NKV+ N GL ICL+DI    D  ++P DGA +                              +VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +D  ++I F+V +  + DT P   S  E     +   +  AP+T+V
Sbjct:    1 MFVLVEMVDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDITKLEDAYVFPGDGASHT-----------------------------KVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTSPTGPSSAEAASSSEELPKKEAPYTLV 158

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Mouse
Match: Polr2g (polymerase (RNA) II (DNA directed) polypeptide G [Source:MGI Symbol;Acc:MGI:1914960])

HSP 1 Score: 54.6842 bits (130), Expect = 2.966e-8
Identity = 39/167 (23.35%), Postives = 73/167 (43.71%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKV--TEYIWQDTFPISNSVDE 523
            MF  ++++  + + PR+F    +  ++Q+L  ++        G  I +  I + G   I P  G     V ++ ++F+PF  E++   V + +  GL   I       I + +IP   EF    N   +   + +I I   D+I  K+  T     D F I + +D+
Sbjct:    1 MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPM-SCFISRHSIPSEMEFDPNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDD 166

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. UniProt/SwissProt
Match: sp|Q9Y535|RPC8_HUMAN (DNA-directed RNA polymerase III subunit RPC8 OS=Homo sapiens OX=9606 GN=POLR3H PE=1 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 6.847e-39
Identity = 76/187 (40.64%), Postives = 111/187 (59.36%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQE--RSTAPFTVV 571
            MF +V + DTVRI P  F+      I +ELN+KL+NKV+ N GL ICL+DI    D  ++P DGA +  V FR V+F PF++EI+ G +  CSPEG+ VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +D  ++I F+V +  + DT P   S  +     +E  +  AP+T+V
Sbjct:    1 MFVLVEMVDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTSPTGPSSADATTSSEELPKKEAPYTLV 187

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. UniProt/SwissProt
Match: sp|Q2T9X1|RPC8_BOVIN (DNA-directed RNA polymerase III subunit RPC8 OS=Bos taurus OX=9913 GN=POLR3H PE=2 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 3.038e-38
Identity = 70/162 (43.21%), Postives = 103/162 (63.58%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFP 502
            MF +V + DTVRI P  F+      I++ELN+KL+NKV+ N GL ICL+DI    D  ++P DGA +  V FRYV+F PF++EI+ G +  CSPEG+ VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +D+ +++ F+V +  + DT P
Sbjct:    1 MFVLVEMVDTVRIPPWQFERKLNDSIDEELNKKLANKVMYNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRYVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDIGEEVRFRVVDESFVDTSP 162

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. UniProt/SwissProt
Match: sp|Q9D2C6|RPC8_MOUSE (DNA-directed RNA polymerase III subunit RPC8 OS=Mus musculus OX=10090 GN=Polr3h PE=1 SV=2)

HSP 1 Score: 142.51 bits (358), Expect = 7.816e-38
Identity = 77/187 (41.18%), Postives = 111/187 (59.36%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQ--ERSTAPFTVV 571
            MF +V + DTVRI P  F+      I +ELN+KL+NKV+ N GL ICL+DI    D  ++P DGA +  V FRYV+F PF++EI+ G +  CSPEG+ VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +D  ++I F+V +  + DT P   S  E     +   +  AP+T+V
Sbjct:    1 MFVLVEMVDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRYVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTSPTGPSSAEAASSSEELPKKEAPYTLV 187

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. UniProt/SwissProt
Match: sp|P35718|RPC8_YEAST (DNA-directed RNA polymerase III subunit RPC8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RPC25 PE=1 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 1.971e-31
Identity = 65/175 (37.14%), Postives = 105/175 (60.00%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVS-IEFFDDIIIPKANIPQSFEFQSEKNSWVWTI-ENGEITIDVQDKINFKVTEYIWQDTFPISNSVDE-NNRVQQE 544
            MF +  I D VRI P  F   T+  I  +LN K +NK++ N GL I ++D+L   + ++ P DG+ Y+ V+FR V+FKPF+ EI+ G ++KC+ EG++VS +  FDDI IP+  + +   +  E+++W+W + E  ++  DV +KI F++   ++ D  P S    E   R Q E
Sbjct:    1 MFILSKIADLVRIPPDQFHRDTISAITHQLNNKFANKIIPNVGLCITIYDLLTVEEGQLKPGDGSSYINVTFRAVVFKPFLGEIVTGWISKCTAEGIKVSLLGIFDDIFIPQNMLFEGCYYTPEESAWIWPMDEETKLYFDVNEKIRFRIEREVFVDVKPKSPKERELEERAQLE 175

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. UniProt/SwissProt
Match: sp|O94285|RPC8_SCHPO (DNA-directed RNA polymerase III subunit rpc8 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rpc25 PE=1 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 3.440e-29
Identity = 63/189 (33.33%), Postives = 105/189 (55.56%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTI------ENGEITIDVQDKINFKVTEYIWQDTFPISN-SVDENNRVQQERSTAPFTVVV 574
            MF +    D + I P +F   T + + +E+++K +NKV+ N GL IC++D L  G+  I   DG+ Y+ V FR +IF+PF  E++ G +  CS EG++V+I FFDDI IPK  +     F+ ++ +WVW I      E  E+  D+ ++I F++    + D  P  N +       +   S +P+T++ 
Sbjct:    1 MFLLSRFSDIISIHPSNFWKPTKEALAEEIHKKYANKVIQNIGLAICVYDFLKIGEGIIKYGDGSSYMNVVFRLIIFRPFRGEVMLGKIKSCSEEGIRVTISFFDDIFIPKDMLFDPCVFRPDERAWVWKIEGEDGSEGTELYFDIDEEIRFQIESEDFVDISPKRNKNATAITGTEALESVSPYTLIA 189

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. TrEMBL
Match: A0A091RK12 (DNA-directed RNA polymerase III subunit RPC8 OS=Merops nubicus OX=57421 GN=N331_08853 PE=4 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 6.051e-39
Identity = 79/189 (41.80%), Postives = 114/189 (60.32%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVDE----NNRVQQERSTAPFTVV 571
            MF +V + DTVRI P  F+      I +ELN+KL+NKV+ N GL ICL+DI    D  I+P DGA +  V FRYV+F PF++EI+ G +  C+PEG+ VSI FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +D+ ++I F+V +  + DT P   S  E    N   + ++  AP+T+V
Sbjct:    1 MFVLVEMTDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLYDITKLEDSYIFPGDGASHTKVHFRYVVFHPFLDEILIGQIKSCTPEGVHVSIGFFDDIVIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDIGEEIRFRVVDETFVDTSPTGPSSAEASTSNATEEVQKKEAPYTLV 189

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. TrEMBL
Match: K9IV91 (Putative dna-directed rna polymerase iii subunit rpc8 (Fragment) OS=Desmodus rotundus OX=9430 PE=2 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 8.739e-39
Identity = 77/187 (41.18%), Postives = 113/187 (60.43%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQE--RSTAPFTVV 571
            MF +V + DTVRI P  F+      I +ELN+KL+NKV+ N GL ICL+DI    D  ++P DGA +  V FRYV+F PF++EI+ G +  CSPEG+ VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +D+ +++ F+V +  + DT P   S  E     +E  +  AP+T+V
Sbjct:    1 MFVLVEMVDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDITKLEDSYVFPGDGASHTKVHFRYVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQMWVWEYETEEGAHDLYMDIGEEVRFRVVDESFVDTSPSGPSSAEAASTSEELPKKEAPYTLV 187

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. TrEMBL
Match: A0A1Y2GJV7 (DNA-directed RNA polymerase III subunit RPC8 OS=Lobosporangium transversale OX=64571 GN=BCR41DRAFT_337925 PE=4 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 1.314e-38
Identity = 79/189 (41.80%), Postives = 110/189 (58.20%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKVTEYIWQDTFPI----SNSVDENNRVQQERSTAPFTVVVRLD 583
            MF++  IKDT++IEP  F+    + I  E+NRK +NKVL N GL I + DIL   +  I+  DG  YVTV FR VIF+PFI EII G +  CS EG++VS+ FFDDI IP   + Q  EF SE+  WVW  +  E+ +D+ + I F+V    + DT P        VD  +        AP+++V  ++
Sbjct:    1 MFNLAEIKDTIKIEPSGFRKKKPQAITDEINRKYANKVLQNVGLCIAMHDILSASEGFIHHGDGCSYVTVHFRMVIFRPFIGEIIIGKIRSCSREGIRVSLGFFDDIFIPAGYLQQGTEFNSEEQVWVWMYDENEMFMDIDESIRFRVESEQFFDTSPSVRPKKEGVDTPDLSANSTKQAPYSLVCSIN 189

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. TrEMBL
Match: M3W6B9 (RNA polymerase III subunit H OS=Felis catus OX=9685 GN=POLR3H PE=4 SV=2)

HSP 1 Score: 150.984 bits (380), Expect = 1.758e-38
Identity = 78/187 (41.71%), Postives = 112/187 (59.89%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQE--RSTAPFTVV 571
            MF +V + DTVRI P  F+      I +ELN+KL+NKV+ N GL ICL+DI    D  ++P DGA +  V FRYV+F PF++EI+ G +  CSPEG+ VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +D  ++I F+V +  + DT P   S  E     +E  +  AP+T+V
Sbjct:    1 MFVLVEMVDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRYVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTSPTGPSSAEGTSSSEELPKKEAPYTLV 187

[Close]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. TrEMBL
Match: A0A4W6DKG8 (Uncharacterized protein OS=Lates calcarifer OX=8187 GN=polr3h PE=4 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 2.587e-38
Identity = 77/192 (40.10%), Postives = 118/192 (61.46%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQERSTAPFTVVVRLDLIV 592
            MF +V + DTVRI P +F+    + I +ELN+KL+NKV+ N GL ICL+DI    D  I+P DGA +  V FRYV+F PF++EI+ G +  CS EG+ V++ FFDDI+IP  ++ Q  +F   +  W+W  E  E    + +D  ++I F+VT+ ++ DT P   +    +  ++E   AP+T++VR    V
Sbjct:    1 MFVLVEMVDTVRIPPWNFQRQLNEAIAEELNKKLANKVVYNVGLCICLYDITKLEDSYIFPGDGASHTKVHFRYVVFHPFLDEILVGKIKYCSQEGVHVTMGFFDDILIPPESLQQPAKFDEAEQVWLWEYETDEGAHDLYMDQGEEIRFRVTDEVFVDTSPTGPATAATDTPKKE---APYTLIVRTHFTV 189

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Cavefish
Match: polr3h (RNA polymerase III subunit H [Source:NCBI gene;Acc:103032147])

HSP 1 Score: 144.436 bits (363), Expect = 1.453e-39
Identity = 75/188 (39.89%), Postives = 110/188 (58.51%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQERSTAPFTVVVRL 580
            MF VV + DTVRI P +F       I +ELN+KL+NKV+ N GL ICL+DI    D  I+P DGA +  V FRYV+F PF++EI+ G +  CS EG+ VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +D  ++I F+V + ++ DT P   + D           +P+T++  +
Sbjct:    1 MFVVVEMVDTVRIPPWNFHRQLNDAIAEELNKKLANKVVYNVGLCICLYDITKLEDSYIFPGDGASHTKVHFRYVVFHPFLDEILVGKIKYCSQEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETDEGAHDLYMDQGEEIRFRVVDELFLDTSPTGPTTDPEKE-------SPYTLMASI 181

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Cavefish
Match: polr3h (RNA polymerase III subunit H [Source:NCBI gene;Acc:103032147])

HSP 1 Score: 143.665 bits (361), Expect = 5.060e-39
Identity = 73/168 (43.45%), Postives = 103/168 (61.31%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPISNSVD 520
            MF VV + DTVRI P +F       I +ELN+KL+NKV+ N GL ICL+DI    D  I+P DGA +  V FRYV+F PF++EI+ G +  CS EG+ VS+ FFDDI+IP  ++ Q  +F   +  WVW  E  E    + +D  ++I F+V + ++ DT P   + D
Sbjct:    1 MFVVVEMVDTVRIPPWNFHRQLNDAIAEELNKKLANKVVYNVGLCICLYDITKLEDSYIFPGDGASHTKVHFRYVVFHPFLDEILVGKIKYCSQEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETDEGAHDLYMDQGEEIRFRVVDELFLDTSPTGPTTD 168

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Cavefish
Match: polr2gl (RNA polymerase II subunit G [Source:NCBI gene;Acc:103034356])

HSP 1 Score: 54.299 bits (129), Expect = 2.983e-8
Identity = 39/167 (23.35%), Postives = 73/167 (43.71%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKV--TEYIWQDTFPISNSVDE 523
            MF  ++++  + + PR+F    +  ++Q+L  ++        G  I +  I + G   I P  G     V ++ ++F+PF  E++   V + +  GL   I       I + +IP   EF    N   +   + +I I   D+I  K+  T     D F I + +D+
Sbjct:    1 MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPM-SCFISRHSIPSEMEFDPNSNPPCYKTVDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDD 166

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Sea Lamprey
Match: POLR3H (RNA polymerase III subunit H [Source:HGNC Symbol;Acc:HGNC:30349])

HSP 1 Score: 103.99 bits (258), Expect = 5.539e-26
Identity = 52/130 (40.00%), Postives = 78/130 (60.00%), Query Frame = 2

Query:  125 KLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEIT----IDVQDKINFKVTEYIWQDTFP 502
             L  +V+ N GL +CL+DI    D  ++P DGA +  V FR+V+F+PF++EI+ G +  CS EG+QVS+ FFDDI IP  ++ Q  +    +  WVW  E  E T    +DV + + F+V +  + DT P
Sbjct:    5 SLFRQVVYNVGLCVCLYDITKMEDSFVFPGDGASHTKVHFRFVVFRPFLDEILIGKIKCCSQEGVQVSLGFFDDIRIPPESLQQPSKSDDGEQVWVWEYETEEGTHDLFMDVGEDVRFRVMDETFVDTTP 134

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Yeast
Match: RPC25 (RNA polymerase III subunit C25; required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p [Source:SGD;Acc:S000001627])

HSP 1 Score: 125.176 bits (313), Expect = 2.838e-33
Identity = 65/175 (37.14%), Postives = 105/175 (60.00%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVS-IEFFDDIIIPKANIPQSFEFQSEKNSWVWTI-ENGEITIDVQDKINFKVTEYIWQDTFPISNSVDE-NNRVQQE 544
            MF +  I D VRI P  F   T+  I  +LN K +NK++ N GL I ++D+L   + ++ P DG+ Y+ V+FR V+FKPF+ EI+ G ++KC+ EG++VS +  FDDI IP+  + +   +  E+++W+W + E  ++  DV +KI F++   ++ D  P S    E   R Q E
Sbjct:    1 MFILSKIADLVRIPPDQFHRDTISAITHQLNNKFANKIIPNVGLCITIYDLLTVEEGQLKPGDGSSYINVTFRAVVFKPFLGEIVTGWISKCTAEGIKVSLLGIFDDIFIPQNMLFEGCYYTPEESAWIWPMDEETKLYFDVNEKIRFRIEREVFVDVKPKSPKERELEERAQLE 175

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Yeast
Match: RPB7 (RNA polymerase II subunit B16; forms dissociable heterodimer with Rpb4p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation [Source:SGD;Acc:S000002812])

HSP 1 Score: 60.4622 bits (145), Expect = 3.710e-11
Identity = 39/156 (25.00%), Postives = 73/156 (46.79%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICL--WDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKVTEYIWQ 490
            MF + ++   + + P  F     + ++ +L  ++        G  +C+  +D +D     I P+DG+    V +R V+FKPF  E++ GTV  CS  G +V +     + + K  +PQ   F +  N   +  ++ E  I ++ +I  K+   I Q
Sbjct:    1 MFFIKDLSLNITLHPSFFGPRMKQYLKTKLLEEVEGSCTGKFGYILCVLDYDNIDIQRGRILPTDGSAEFNVKYRAVVFKPFKGEVVDGTVVSCSQHGFEVQVGPM-KVFVTKHLMPQDLTFNAGSNPPSY--QSSEDVITIKSRIRVKIEGCISQ 153

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Nematostella
Match: EDO38289 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDD0])

HSP 1 Score: 143.28 bits (360), Expect = 1.648e-39
Identity = 75/190 (39.47%), Postives = 113/190 (59.47%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTI-----ENGEITIDVQDKINFKVTEYIWQDTFPISNSVDENNRV----QQERSTAPFTVV 571
            MF +  IKDTV+I+P  F L   + I  +LN KL+NKV+ N GL I L DIL  GD  + P DG+ +  V+FR+V+F+PF++EI+ G +  C+ EG+QVS+EFFDDIIIP  N+ Q  +F  ++  WVW       E   + +DV ++I F+V +  + D  P        +++     QE   +P+T+ 
Sbjct:    1 MFVLAEIKDTVQIKPWLFSLDIQEAISNKLNMKLANKVVHNVGLCIVLHDILSIGDSYLVPGDGSSHTPVTFRFVVFRPFVDEILKGRIRSCNHEGVQVSMEFFDDIIIPYENLQQPSKFDEDEQVWVWEYTQEGDETHSMFMDVNEEIRFRVVDETFTDVTPSGPEAASKDQIATKDDQEIRKSPYTIT 190

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BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Nematostella
Match: EDO39159 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAY0])

HSP 1 Score: 51.6026 bits (122), Expect = 1.047e-7
Identity = 35/167 (20.96%), Postives = 74/167 (44.31%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKV--TEYIWQDTFPISNSVDE 523
            MF  ++++  + + PR+F    ++ ++Q+L  ++        G  I +  I + G   I P  G     V ++ ++F+PF  E++   + + +  GL   I       + + +IP   EF    N   +  ++ ++     D+I  +V  T     D F I + +D+
Sbjct:    1 MFYHISLEHEILLHPRYFGPNLLQTVKQKLFTEVEGTCSGKYGFIIAVTTIDNIGVGHIQPGRGFVVYPVKYKAIVFRPFKGEVLDAVITQVNKLGLFTEIGPL-SCFVSRHSIPPDMEFDPNSNPPCYKTKDEDVVYQQDDEIRLRVVGTRVDANDIFAIGSLMDD 166

[Close]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Medaka
Match: polr3h (RNA polymerase III subunit H [Source:NCBI gene;Acc:101173470])

HSP 1 Score: 142.51 bits (358), Expect = 1.295e-38
Identity = 73/181 (40.33%), Postives = 109/181 (60.22%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGE----ITIDVQDKINFKVTEYIWQDTFPIS-NSVDENNRVQQERSTA 556
            MF +V + DTVRI P  F     + + +ELN+KL+NKV+ N GL ICL+DI    D  I+P DGA +  V FR+V+F PF+ EI+ G +  CS EG+ V++ FFDDI+IP  ++ Q  +F   +  W+W  E  E    + +D  ++I F+VT+ ++ DT P    +   +N  Q  +STA
Sbjct:    1 MFVLVEMVDTVRIPPWCFHRQLNEAVAEELNKKLANKVVFNVGLCICLYDITKLEDSYIFPGDGASHTKVHFRFVVFHPFLEEILVGKIKYCSQEGVHVTMGFFDDILIPPESLQQPAKFDEAEQVWLWEYETDEGAHDLYMDQGEEIRFRVTDEVFVDTSPTGPAAATPDNAAQPGQSTA 181

[Close]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Medaka
Match: polr2gl (RNA polymerase II subunit G [Source:NCBI gene;Acc:101162220])

HSP 1 Score: 54.299 bits (129), Expect = 2.965e-8
Identity = 39/167 (23.35%), Postives = 73/167 (43.71%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKV--TEYIWQDTFPISNSVDE 523
            MF  ++++  + + PR+F    +  ++Q+L  ++        G  I +  I + G   I P  G     V ++ ++F+PF  E++   V + +  GL   I       I + +IP   EF    N   +   + +I I   D+I  K+  T     D F I + +D+
Sbjct:    1 MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPM-SCFISRHSIPSEMEFDPNSNPPCYKTVDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDD 166

[Close]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Medaka
Match: polr2gl (RNA polymerase II subunit G [Source:NCBI gene;Acc:101162220])

HSP 1 Score: 53.9138 bits (128), Expect = 5.475e-8
Identity = 39/169 (23.08%), Postives = 74/169 (43.79%), Query Frame = 2

Query:   23 ILMFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKV--TEYIWQDTFPISNSVDE 523
            I+M   ++++  + + PR+F    +  ++Q+L  ++        G  I +  I + G   I P  G     V ++ ++F+PF  E++   V + +  GL   I       I + +IP   EF    N   +   + +I I   D+I  K+  T     D F I + +D+
Sbjct:    2 IMMCLQISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPM-SCFISRHSIPSEMEFDPNSNPPCYKTVDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDD 169

[Close]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Planmine SMEST
Match: SMESG000019826.1 (SMESG000019826.1)

HSP 1 Score: 370.548 bits (950), Expect = 3.578e-126
Identity = 184/194 (94.85%), Postives = 187/194 (96.39%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQERSTAPFTVVVRLDLIVVAISLP 610
            MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQERSTAPFTVV  ++  V  + LP
Sbjct:    1 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKVTEYIWQDTFPISNSVDENNRVQQERSTAPFTVVGTIN--VHGLGLP 192

[Close]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Planmine SMEST
Match: SMESG000034943.1 (SMESG000034943.1)

HSP 1 Score: 48.1358 bits (113), Expect = 3.515e-6
Identity = 30/148 (20.27%), Postives = 66/148 (44.59%), Query Frame = 2

Query:   29 MFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLNTGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAKCSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQDKINFKV 472
            MF  +N+K  + + P++     ++ I+ +L  ++      N G  I +  I   GD  +    G   + + ++ ++F+PF+ E++   V   +  G+      F  I I    +PQ+F++  +     +  E  +  I   D+I  ++
Sbjct:    1 MFYHLNLKHEINLHPKYLGPNIIETIKAKLLSEVEGICTPNYGFIIAVTSIDKIGDGVVQQGRGFTLIPIEYKAIVFRPFVGEVLDAVVLNVTKIGIFAMAGAF-QIFISVYAVPQNFQYDGDTVPPCYKTEEDDQVIQEDDEIRVRI 147

The following BLAST results are available for this feature:

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Human
BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Celegans
BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Fly
BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Zebrafish
BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Xenopus
BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Mouse
BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. UniProt/SwissProt
BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. TrEMBL
BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Cavefish
BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Sea Lamprey
BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Yeast
BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Nematostella
BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Medaka
BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Planmine SMEST

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
POLR3H	1.426e-39	40.64	RNA polymerase III subunit H [Source:HGNC Symbol;A...	[more]
POLR3H	1.426e-39	40.64	RNA polymerase III subunit H [Source:HGNC Symbol;A...	[more]
POLR3H	1.426e-39	40.64	RNA polymerase III subunit H [Source:HGNC Symbol;A...	[more]
POLR3H	1.103e-23	47.96	RNA polymerase III subunit H [Source:HGNC Symbol;A...	[more]
POLR3H	7.061e-22	32.62	RNA polymerase III subunit H [Source:HGNC Symbol;A...	[more]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rpc-25	1.432e-27	35.37	RNA Polymerase, Class III (C) [Source:UniProtKB/T...	[more]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3


Match Name	E-value	Identity	Description
CG7339	6.396e-38	41.14	gene:FBgn0036188 transcript:FBtr0331530	[more]
CG7339	6.396e-38	41.14	gene:FBgn0036188 transcript:FBtr0076107	[more]
Rpb7	4.621e-7	21.62	gene:FBgn0051155 transcript:FBtr0083137	[more]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
polr3h	6.169e-41	43.79	polymerase (RNA) III (DNA directed) polypeptide H ...	[more]
polr2gl	5.719e-8	22.75	polymerase (RNA) II (DNA directed) polypeptide G-l...	[more]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4


Match Name	E-value	Identity	Description
myadml2	3.385e-40	40.80	myeloid-associated differentiation marker-like 2 [...	[more]
myadml2	8.780e-39	43.29	myeloid-associated differentiation marker-like 2 [...	[more]
polr2g	8.149e-8	22.75	polymerase (RNA) II (DNA directed) polypeptide G [...	[more]
polr2g	2.075e-6	21.53	polymerase (RNA) II (DNA directed) polypeptide G [...	[more]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3


Match Name	E-value	Identity	Description
Polr3h	1.117e-38	41.18	polymerase (RNA) III (DNA directed) polypeptide H ...	[more]
Polr3h	6.913e-20	32.62	polymerase (RNA) III (DNA directed) polypeptide H ...	[more]
Polr2g	2.966e-8	23.35	polymerase (RNA) II (DNA directed) polypeptide G [...	[more]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q9Y535\|RPC8_HUMAN	6.847e-39	40.64	DNA-directed RNA polymerase III subunit RPC8 OS=Ho...	[more]
sp\|Q2T9X1\|RPC8_BOVIN	3.038e-38	43.21	DNA-directed RNA polymerase III subunit RPC8 OS=Bo...	[more]
sp\|Q9D2C6\|RPC8_MOUSE	7.816e-38	41.18	DNA-directed RNA polymerase III subunit RPC8 OS=Mu...	[more]
sp\|P35718\|RPC8_YEAST	1.971e-31	37.14	DNA-directed RNA polymerase III subunit RPC8 OS=Sa...	[more]
sp\|O94285\|RPC8_SCHPO	3.440e-29	33.33	DNA-directed RNA polymerase III subunit rpc8 OS=Sc...	[more]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A091RK12	6.051e-39	41.80	DNA-directed RNA polymerase III subunit RPC8 OS=Me...	[more]
K9IV91	8.739e-39	41.18	Putative dna-directed rna polymerase iii subunit r...	[more]
A0A1Y2GJV7	1.314e-38	41.80	DNA-directed RNA polymerase III subunit RPC8 OS=Lo...	[more]
M3W6B9	1.758e-38	41.71	RNA polymerase III subunit H OS=Felis catus OX=968...	[more]
A0A4W6DKG8	2.587e-38	40.10	Uncharacterized protein OS=Lates calcarifer OX=818...	[more]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
polr3h	1.453e-39	39.89	RNA polymerase III subunit H [Source:NCBI gene;Acc...	[more]
polr3h	5.060e-39	43.45	RNA polymerase III subunit H [Source:NCBI gene;Acc...	[more]
polr2gl	2.983e-8	23.35	RNA polymerase II subunit G [Source:NCBI gene;Acc:...	[more]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
POLR3H	5.539e-26	40.00	RNA polymerase III subunit H [Source:HGNC Symbol;A...	[more]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2


Match Name	E-value	Identity	Description
RPC25	2.838e-33	37.14	RNA polymerase III subunit C25; required for trans...	[more]
RPB7	3.710e-11	25.00	RNA polymerase II subunit B16; forms dissociable h...	[more]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO38289	1.648e-39	39.47	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO39159	1.047e-7	20.96	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
polr3h	1.295e-38	40.33	RNA polymerase III subunit H [Source:NCBI gene;Acc...	[more]
polr2gl	2.965e-8	23.35	RNA polymerase II subunit G [Source:NCBI gene;Acc:...	[more]
polr2gl	5.475e-8	23.08	RNA polymerase II subunit G [Source:NCBI gene;Acc:...	[more]

BLAST of DNA-directed RNA polymerase III subunit RPC8 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000019826.1	3.578e-126	94.85	SMESG000019826.1	[more]
SMESG000034943.1	3.515e-6	20.27	SMESG000034943.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30013558 ID=SMED30013558|Name=DNA-directed RNA polymerase III subunit RPC8|organism=Schmidtea mediterranea sexual|type=transcript|length=1864bp
ACTATACATTTTTAAACTATACATTTTAATGTTTGACGTAGTTAATATTA
AAGATACAGTTCGCATTGAGCCTAGACATTTTAAATTAGGAACAGTAAAG
TGCATTGAACAAGAGTTAAATAGAAAACTTTCAAATAAAGTTTTACTAAA
TACTGGTTTGGGCATATGTTTGTGGGATATTTTAGATTATGGGGATCGCG
AAATTTATCCCAGTGATGGTGCATATTACGTTACAGTTAGTTTTAGATAT
GTAATTTTTAAACCATTTATTAACGAAATTATTGCTGGAACTGTTGCAAA
ATGTTCCCCAGAAGGTCTTCAGGTGTCGATAGAATTTTTCGATGATATTA
TTATACCTAAGGCAAATATTCCTCAGTCATTCGAATTCCAGTCAGAAAAG
AATTCCTGGGTTTGGACAATTGAAAATGGGGAAATCACCATCGATGTACA
GGATAAAATCAACTTCAAGGTTACAGAATATATCTGGCAGGATACTTTCC
CAATATCGAATTCGGTCGATGAGAACAATCGAGTTCAGCAGGAGAGGTCG
ACGGCTCCATTCACTGTTGTGGTAAGGCTGGATTTGATTGTCGTGGCGAT
TTCATTACCGTTACCAGGGTACCATAAACGTGCACGGATTGGGACTGCCG
TGCTGGTGGGATGACAACTGATGGCAAACATCGATTTCCTTAATAAATAA
TTAGATTGTCTATTTCATCCAATCACAGCGAATGCGGCCTGCTATTGGTC
AGTTTTCTAGAATGTCTGCAGCCAATCGTTTTTCATCTAATCAATGGTTG
CTCTTTATTTATTTTCTACTGTTTTCTGGTTGGTTTTACGTGTAATCTGC
TTGCGTTCATATTTTGTTTAAATTTTACTGTTCTTGTGTCGGTTGCCTTT
CACTTTTGGCTATTTTTAATGATTAACTTGGTGGGTTCCAGTCCAACCGA
GGGATAAGGAGAAGGCCAAAACGGTTGGTGAGAGACACACTAGCTATCAG
CACCTCTGTAAACCCAGAGGTGCTCCGTGTCTGACAGTCAGCCATTTTAC
ACAGTTCTAAACATCACTAGCAACTAATGTCAATTGGTAGAAGGAGAAAG
CTACATTTGGTTTTTTAGGCATGTAAACTCATTTTTTTGTACAAGTTAGG
TTGAAATTGATGCTAAGTTTATTTAAAATCACTGCTCTTATGCTCGTTTA
AGCAATCACATAGGTATTGGTATCAAAAGAATTTGGTATCAAAACCTTGA
AAGCAAAAAGTTTAGATGTCAAACCCTTGCATAGACAATCTTAGTTTAGC
ATAGTTCTAATTATGAACAAGGAAATTAAAATTGCCCAAATCCTTATATG
AATAGATGAGAATCCATTAAGGAGAATTAGTAAGGAGTAGCTTTGGCGAC
GGAGATCAAATGTCTCGTGGTTTGCAACCTTATTGCATATTTCTGATATC
GTGGTATATATGCGCTTTTATCGTTAATTAATTGCAGAAATCATTGGACG
TTTTGAGATTAATTACAACCAGGGCAAATTAATGACCCAAAAAGAAAACA
TCAGCAGAATTGTAAGCATTAATTGCTAATTATAGAGTTCTTGATCAACC
TATAGAAAGTAAAAATCATTTCTTCGGCATTTAAGCTTCAAGATTTGCAT
GCTCTATTCATTCACAGCGATAATTTCAATATCATTTTGAATCATTGTCC
TTATTACCTGAACAAACAGTTGTTCTCTCACCGAATGAATTACAGTGAAT
AAGTGATGTAATCCGCTCAAATATTAATAACAGAATCTATTGTTGATGAA
AATCTTTAAACGAATTTCAAAAAATATTAATTTGAGCTTTACTGTTTGAA
ATGTATAACCTTAC

protein sequence of SMED30013558-orf-1

>SMED30013558-orf-1 ID=SMED30013558-orf-1|Name=SMED30013558-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=221bp
LYIFKLYILMFDVVNIKDTVRIEPRHFKLGTVKCIEQELNRKLSNKVLLN
TGLGICLWDILDYGDREIYPSDGAYYVTVSFRYVIFKPFINEIIAGTVAK
CSPEGLQVSIEFFDDIIIPKANIPQSFEFQSEKNSWVWTIENGEITIDVQ
DKINFKVTEYIWQDTFPISNSVDENNRVQQERSTAPFTVVVRLDLIVVAI
SLPLPGYHKRARIGTAVLVG*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0002109	X1 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR036898	RNA_pol_Rpb7-like_N_sf
IPR013238	RNA_pol_III_Rbc25
IPR012340	NA-bd_OB-fold
IPR005576	Rpb7-like_N

Vocabulary: **molecular function**
Term	Definition
GO:0003899	DNA-directed 5'-3' RNA polymerase activity
GO:0003677	DNA binding

Vocabulary: **biological process**
Term	Definition
GO:0006351	transcription, DNA-templated
GO:0002376	immune system process
GO:0006384	transcription initiation from RNA polymerase III promoter
GO:0045087	innate immune response
GO:0051607	defense response to virus

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus
GO:0005666	DNA-directed RNA polymerase III complex

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036898	RNA polymerase Rpb7-like, N-terminal domain superfamily	GENE3D	G3DSA:3.30.1490.120		coord: 10..88 e-value: 1.8E-25 score: 90.7
IPR036898	RNA polymerase Rpb7-like, N-terminal domain superfamily	SUPERFAMILY	SSF88798	N-terminal, heterodimerisation domain of RBP7 (RpoE)	coord: 10..87
None	No IPR available	GENE3D	G3DSA:2.40.50.140		coord: 89..195 e-value: 4.8E-23 score: 83.9
None	No IPR available	PANTHER	PTHR12709	DNA-DIRECTED RNA POLYMERASE II, III	coord: 10..190
None	No IPR available	PANTHER	PTHR12709:SF1	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8	coord: 10..190
IPR013238	RNA polymerase III, subunit Rpc25	PFAM	PF08292	RNA_pol_Rbc25	coord: 88..181 e-value: 7.7E-24 score: 84.4
IPR005576	RNA polymerase Rpb7-like , N-terminal	PFAM	PF03876	SHS2_Rpb7-N	coord: 17..86 e-value: 3.6E-12 score: 46.5
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	SSF50249	Nucleic acid-binding proteins	coord: 88..163