Trypsin-like serine protease

Overview
NameTrypsin-like serine protease
Smed IDSMED30013511
Length (bp)841
Neoblast Clusters

Zeng et. al., 2018




 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30013511

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30013511h1SMcG0015727 SmedASXL_003301SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30013511h1SMcG0015727 SmedASXL_003301SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pigment cellSMED30013511h1SMcG0015727 SmedASXL_003301SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30013511h1SMcG0015727 dd_Smed_v6_4570_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30013511h1SMcG0015727 OX_Smed_1.0.15926ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Trypsin-like serine protease vs. Ensembl Human
Match: KLK13 (kallikrein related peptidase 13 [Source:HGNC Symbol;Acc:HGNC:6361])

HSP 1 Score: 120.939 bits (302), Expect = 3.994e-32
Identity = 85/250 (34.00%), Postives = 125/250 (50.00%), Query Frame = 3
Query:   72 NGTQSIL--------HEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLN-GNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSN-LCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            NGT   L        H  P   ++ + G  +CGG L++   VLTAAHC+        E LK Y     LG++ L RV   E++ +V  ++ HP+Y    ++LN  +D+ L++    VQ     YI+     + +    G  C V GWG+         + LQ A++ + S  EC  ++     I  N LCAG+      +C+GDSGGPL C    N+ +  GIVS+G  PCGQP+ P V+T+VS    WI
Sbjct:   30 NGTSGFLPGGYTCFPHSQPWQAALLVQGRLLCGGVLVHPKWVLTAAHCL-------KEGLKVY-----LGKHALGRVEAGEQVREVVHSIPHPEYRRSPTHLNHDHDIMLLELQSPVQLTG--YIQTLPLSHNNRLTPGTTCRVSGWGTTTSPQVNYPKTLQCANIQLRSDEECRQVYPG--KITDNMLCAGTKEGGKDSCEGDSGGPLVC----NRTL-YGIVSWGDFPCGQPDRPGVYTRVSRYVLWI 258          
BLAST of Trypsin-like serine protease vs. Ensembl Human
Match: PRSS38 (serine protease 38 [Source:HGNC Symbol;Acc:HGNC:29625])

HSP 1 Score: 117.857 bits (294), Expect = 1.696e-30
Identity = 80/251 (31.87%), Postives = 130/251 (51.79%), Query Frame = 3
Query:   51 SAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNE--FQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYI-PSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKN-IQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            S E K++ G  +   ++P  VS+   G H+CGG++LNE  VL+AAHC       + + +K Y  ++ +G  +L       + ++V + ++HP  TYEM +  G DV L++   ++  +E        T   N+++      C   GWG +   G  S   LQ+  L ++    C  ++  + YI P  LCAG    +   C+GDSGGPL C+  +N++ +Q+GIVS+G   C  P  P V+  VS  + WI
Sbjct:   55 SMEGKILGGVPAPERKWPWQVSVHYAGLHVCGGSILNEYWVLSAAHCF-----HRDKNIKIY--DMYVGLVNLRVAGNHTQWYEVNRVILHP--TYEMYHPIGGDVALVQLKTRIVFSESVLPVCLATPEVNLTS----ANCWATGWGLVSKQGETSDE-LQEMQLPLILEPWCHLLYGHMSYIMPDMLCAGDILNAKTVCEGDSGGPLVCE--FNRSWLQIGIVSWG-RGCSNPLYPGVYASVSYFSKWI 288          
BLAST of Trypsin-like serine protease vs. Ensembl Human
Match: GZMK (granzyme K [Source:HGNC Symbol;Acc:HGNC:4711])

HSP 1 Score: 115.161 bits (287), Expect = 4.639e-30
Identity = 77/264 (29.17%), Postives = 131/264 (49.62%), Query Frame = 3
Query:   21 IVFLI---FISTVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYEC--AYIFTELPYIPSNL-CAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVS-EVANWI 791
            + FLI   +++ V    ++I G +   H  P M SI+  G H+CGG L++   VLTAAHC +  T  +  T       +VLG + L +    ++  ++ K +    ++   S    ND+ L+K     + N  +++K    ++ ++   G +C+V GWG+ DP     S  L++ ++ ++S   C     +   P+I  ++ CAG       +CKGDSGGPL CK  ++      IVS G E CG    P ++T ++ +   WI
Sbjct:    9 LFFLIVGAYMTHVCFNMEIIGGKEVSPHSRPFMASIQYGGHHVCGGVLIDPQWVLTAAHCQYRFTKGQSPT-------VVLGAHSLSKNEASKQTLEIKKFI---PFSRVTSDPQSNDIMLVKLQTAAKLN--KHVKMLHIRSKTSLRSGTKCKVTGWGATDPDSLRPSDTLREVTVTVLSRKLCNSQSYYNGDPFITKDMVCAGDAKGQKDSCKGDSGGPLICKGVFH-----AIVSGGHE-CGVATKPGIYTLLTKKYQTWI 254          
BLAST of Trypsin-like serine protease vs. Ensembl Human
Match: CTRL (chymotrypsin like [Source:HGNC Symbol;Acc:HGNC:2524])

HSP 1 Score: 113.235 bits (282), Expect = 2.536e-29
Identity = 76/246 (30.89%), Postives = 123/246 (50.00%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIR-LLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWITR 797
            +++NG  ++L  +P  VS++   G H CGG+L++++ V+TAAHC           +   +  +VLGEYD    +   ++  V++ + HP +    S    NDV L+K     Q             N +  E G  C   GWG +   G  +   LQ+ +L +V+  +C   +       S +CAG    S+  C+GDSGGPL C+ G N  + +GIVS+G + C     P V+T+VS+ + WI +
Sbjct:   33 RIVNGENAVLGSWPWQVSLQDSSGFHFCGGSLISQSWVVTAAHC----------NVSPGRHFVVLGEYDRSSNAEPLQVLSVSRAITHPSWN---STTMNNDVTLLKLASPAQYTTRISPVCLASSNEALTE-GLTCVTTGWGRLSGVGNVTPAHLQQVALPLVTVNQCRQYWGS-SITDSMICAGGAGASS--CQGDSGGPLVCQKG-NTWVLIGIVSWGTKNC-NVRAPAVYTRVSKFSTWINQ 259          
BLAST of Trypsin-like serine protease vs. Ensembl Human
Match: KLK3 (kallikrein related peptidase 3 [Source:HGNC Symbol;Acc:HGNC:6364])

HSP 1 Score: 112.079 bits (279), Expect = 5.910e-29
Identity = 84/280 (30.00%), Postives = 128/280 (45.71%), Query Frame = 3
Query:   15 ILIVFLIFISTVSAEW---------KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYL----------NGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYE--IGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            + +VFL    T+S  W         +++ G +   H  P  V +   G  +CGG L++   VLTAAHCI              K  I+LG + L       ++FQV+ +  HP   Y+MS L          + +D+ L++      +   +     +  ++ T E  +G  C   GWGSI+P    + + LQ   L+++S+  CA +  +       LCAG      + C GDSGGPL C NG    +  GI S+G EPC  P  P+++TKV     WI
Sbjct:    3 VPVVFL----TLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCI------------RNKSVILLGRHSLFHPEDTGQVFQVSHSFPHP--LYDMSLLKNRFLRPGDDSSHDLMLLRL-----SEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQ-KVTKFMLCAGRWTGGKSTCSGDSGGPLVC-NG----VLQGITSWGSEPCALPERPSLYTKVVHYRKWI 253          
BLAST of Trypsin-like serine protease vs. Ensembl Celegans
Match: try-1 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q23528])

HSP 1 Score: 96.2857 bits (238), Expect = 3.327e-23
Identity = 73/251 (29.08%), Postives = 121/251 (48.21%), Query Frame = 3
Query:   48 VSAEWKVINGTQSILHEYPQMVSI-RLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYI--FTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            V+ + ++I G++S  H +P  V +   LG H CGG+L++ N VLTAAHC       K      Y V +  G +     SG     +VT   +HP   Y + + +  D  +++    V  N     +     ++   E    C V GWGS       S+  L++  + ++S+  C+ +  +    ++PS LCAG  +    +C+GDSGGPL C    +  +  G+VS+G+  C +P +P V+  V   + WI
Sbjct:   52 VTLDHRLIGGSESSPHSWPWTVQLLSRLGHHRCGGSLIDPNFVLTAAHCFA-----KDRRPTSYSVRV--GGHR----SGSGSPHRVTAVSIHP--WYNIGFPSSYDFAIMRIHPPV--NTSTTARPICLPSLPAVE-NRLCVVTGWGSTIEGSSLSAPTLREIHVPLLSTLFCSSLPNYIGRIHLPSMLCAGYSYGKIDSCQGDSGGPLMCARDGHWEL-TGVVSWGIG-CARPGMPGVYGNVHSASTWI 284          
BLAST of Trypsin-like serine protease vs. Ensembl Celegans
Match: svh-1 (HGF/MSP/plasminogen-like protein [Source:UniProtKB/TrEMBL;Acc:Q17800])

HSP 1 Score: 93.2041 bits (230), Expect = 3.881e-21
Identity = 69/256 (26.95%), Postives = 127/256 (49.61%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIR--LLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEY-QNIS------TYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYEC---AYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWIT 794
            +V+ G +++   +P   ++R      H CG ++L++  ++TAAHC       + E +  Y  E+V+G++D  +  G E+IF + +   +P Y    S+    D+ +++         +  I++ EY Q I        Y  G +C V GWGS+   G   +  LQ A + I++ ++C   + I++ +    S  CAG       +C+GDSGGP  C+      +  G++S+G + C Q   P ++T V+   +WI+
Sbjct:  712 RVVGGFETVPGAFPWTAALRNKATKAHHCGASILDKTHLITAAHCFE-----EDERVSSY--EVVVGDWDNNQTDGNEQIFYLQRIHFYPLYKDIFSH----DIAILEI-------PYPGIEFNEYAQPICLPSKDFVYTPGRQCVVSGWGSM---GLRYAERLQAALIPIINRFDCVNSSQIYSSMSR--SAFCAGYLEGGIDSCQGDSGGPFACRREDGAFVLAGVISWG-DGCAQKKQPGIYTMVAPYLSWIS 943          
BLAST of Trypsin-like serine protease vs. Ensembl Celegans
Match: try-3 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q94176])

HSP 1 Score: 63.929 bits (154), Expect = 1.182e-11
Identity = 59/235 (25.11%), Postives = 105/235 (44.68%), Query Frame = 3
Query:  138 ICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYI--KYTEYQNISTYEIGERCEVVGWGSI---DPSGRG---SSRFLQKASLNIVSSYECAYIFTELPYIPS-----NLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPC----GQPNVPTVFTKVSEVANWI 791
            +CG T++++  ++TAAHC   +       ++E K             +  E+ F V +  +H  Y  + +    ND+ L++    +     + +   + + + +  Y+ G    V+G+G     D SG     +S+ LQ  S+ I+S  +C   +  L  +        +CAG+    TA   GDSGGPL       + +Q+GI SYG +       Q   P V+T++S+   WI
Sbjct:   15 LCGATVIDDFWLVTAAHCALQLQTRSFVYVREPK-------------NNRERSFSVKEAYIHSGYNNQTA---DNDIALLRISSDLSKLGIKPVCLVHDDSKLLKQYKNGV---VIGYGLTLGEDSSGEPKLINSQTLQSTSVPIISDDDCVKTWRFLSLLSVKITGYQICAGAYLHGTAP--GDSGGPLLIHKSNGEYVQIGITSYGADGLDGVIDQGKFPGVYTRISKYVPWI 228          
BLAST of Trypsin-like serine protease vs. Ensembl Celegans
Match: try-3 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q94176])

HSP 1 Score: 63.929 bits (154), Expect = 1.597e-11
Identity = 59/235 (25.11%), Postives = 105/235 (44.68%), Query Frame = 3
Query:  138 ICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYI--KYTEYQNISTYEIGERCEVVGWGSI---DPSGRG---SSRFLQKASLNIVSSYECAYIFTELPYIPS-----NLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPC----GQPNVPTVFTKVSEVANWI 791
            +CG T++++  ++TAAHC   +       ++E K             +  E+ F V +  +H  Y  + +    ND+ L++    +     + +   + + + +  Y+ G    V+G+G     D SG     +S+ LQ  S+ I+S  +C   +  L  +        +CAG+    TA   GDSGGPL       + +Q+GI SYG +       Q   P V+T++S+   WI
Sbjct:   65 LCGATVIDDFWLVTAAHCALQLQTRSFVYVREPK-------------NNRERSFSVKEAYIHSGYNNQTA---DNDIALLRISSDLSKLGIKPVCLVHDDSKLLKQYKNGV---VIGYGLTLGEDSSGEPKLINSQTLQSTSVPIISDDDCVKTWRFLSLLSVKITGYQICAGAYLHGTAP--GDSGGPLLIHKSNGEYVQIGITSYGADGLDGVIDQGKFPGVYTRISKYVPWI 278          
BLAST of Trypsin-like serine protease vs. Ensembl Celegans
Match: try-10 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:E1B6T9])

HSP 1 Score: 51.6026 bits (122), Expect = 2.172e-7
Identity = 57/255 (22.35%), Postives = 105/255 (41.18%), Query Frame = 3
Query:   27 FLIFISTVSAEWKVING----------TQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLI----KFDGKVQNNEFQYIKYTEYQNISTYEIG---------ERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFC-KNGYNKNIQVGIVSY 719
            F++ +S +S    +ING            S++  +P   +      ++CGG L+  + V+T+AHC++   D+ +        ++ LG+  L +    E+ F+     +   +  + S  N +DV +I    + D        Q  K     +++  E             C V GWG  +      S  +++  +N+           +  Y+ +    G    S+ AC GDSG P++C  NG  K I VG V++
Sbjct:    4 FILLLSFISYSTSIINGFSANSFDTLSLASVITRFPDGTT------NVCGGVLIAPSIVITSAHCVFSGDDFAV------TAKVTLGDVHLNKHDDGEQEFRSHAMAISKKFFNDASEAN-DDVAVIFLPQRADVCHSPLSLQIAKLPSTGSVNFKETAPLTQLQLETSVCYVAGWGKTENKTAKYSDSVRQMMVNL-----SVRRIGKRKYLIAKAVTG----SSRACMGDSGSPVYCFVNG--KRILVGTVAH 234          
BLAST of Trypsin-like serine protease vs. Ensembl Fly
Match: CG32808 (gene:FBgn0052808 transcript:FBtr0070295)

HSP 1 Score: 118.627 bits (296), Expect = 2.108e-31
Identity = 84/247 (34.01%), Postives = 125/247 (50.61%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLL--GEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEF-QYIKYTEYQNISTYEIGERCEVV-GWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            K++NGT +   E+P +VS+R    G H CG TLLN   VLTAAHC+              ++++  G   L R S   ++ +V    VHP Y  E  Y+N  D+ L++    V  ++F Q ++  E + ++    G    V+ GWG ++ +G    + LQK  L + S  EC+       +  S +CAG        C GDSGGPL        + QVGIVS+ ++PC +P  P VFT+VS   +WI
Sbjct:   29 KIVNGTTAGPGEFPFVVSLRRAKSGRHSCGATLLNPYWVLTAAHCV--------RGSSPEQLDLQYGSQMLARNS--SQVARVAAIFVHPGYEPEDKYVN--DIALLQLAQSVALSKFVQPVRLPEPRQVTP---GNASAVLAGWG-LNATGGVVQQHLQKVKLQVFSDTECSERHQTYLH-DSQICAGLPEGGKGQCSGDSGGPLLL---IGSDTQVGIVSWSIKPCARPPFPGVFTEVSAYVDWI 255          
BLAST of Trypsin-like serine protease vs. Ensembl Fly
Match: CG11911 (gene:FBgn0031249 transcript:FBtr0078098)

HSP 1 Score: 108.997 bits (271), Expect = 6.781e-28
Identity = 80/249 (32.13%), Postives = 121/249 (48.59%), Query Frame = 3
Query:   66 VINGTQSILHEYPQMVSIR---LLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCE--VVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTE-LPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWIT 794
            VINGT++  H  P +VS+    L   HICGGTL+N++ ++TAAHCI           +   + I+ G +    V    +  QV    VH  YT     +   D+ L+       N  F + ++ +   + + E     E  + GWG         ++ LQ  +  I++  EC     E  P   SN+C+ S   S +AC GDSGGPL  +     +  +GIVS+G  PCG  N+P+++TKVS   +WIT
Sbjct:   37 VINGTEAEPHSAPYIVSLATNYLKHSHICGGTLINKDWIVTAAHCI----------SEPVGMSIIAGLHTRAEVDELTQQRQVDFGRVHEKYT---GGVGPYDIALLHV-----NESFIFNEWVQPATLPSREQVHEGETHLYGWGQPKSYIFSGAKTLQTVTTQILNYEECKEELPESAPIAESNICSSSLQQSKSACNGDSGGPLVVEFTNAPSELIGIVSWGYIPCGLANMPSIYTKVSAYIDWIT 267          
BLAST of Trypsin-like serine protease vs. Ensembl Fly
Match: l(2)k05911 (gene:FBgn0284244 transcript:FBtr0305556)

HSP 1 Score: 110.153 bits (274), Expect = 6.869e-27
Identity = 80/249 (32.13%), Postives = 127/249 (51.00%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQ-NNEFQYI--KYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELP---YIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            +++ G  +  HE+P +  +   G+  CGG+L+  + +LTAAHC+  MT W +  L  +     LG+Y++      + + +  K LV     +E S L+ NDV ++     V    E Q I    +  Q   +Y  G+   V GWGS+  +G   S  LQK  + I ++ ECA  +        I S +CA  G  +  +C GDSGGP+   +G  +  QVGIVS+G+  CG+   P V+T+V+ +  WI
Sbjct:  399 RIVGGINASPHEFPWIAVLFKSGKQFCGGSLITNSHILTAAHCVARMTSWDVAALTAH-----LGDYNIGTDFEVQHVSRRIKRLVR-HKGFEFSTLH-NDVAILTLSEPVPFTREIQPICLPTSPSQQSRSYS-GQVATVAGWGSLRENGPQPS-ILQKVDIPIWTNAECARKYGRAAPGGIIESMICA--GQAAKDSCSGDSGGPMVINDG-GRYTQVGIVSWGIG-CGKGQYPGVYTRVTSLLPWI 634          
BLAST of Trypsin-like serine protease vs. Ensembl Fly
Match: Sb (gene:FBgn0003319 transcript:FBtr0344008)

HSP 1 Score: 108.612 bits (270), Expect = 2.821e-26
Identity = 81/258 (31.40%), Postives = 125/258 (48.45%), Query Frame = 3
Query:   57 EWKVINGTQSILHEYPQMVSIR------LLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQ--VTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEF-QYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTEL---PYIPS-NLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            E +++ G  +    +P  VS+R          H CGG L+NEN + TA HC+        + L   ++ I +GEYD   V  +    +  V K +VHP Y++ ++Y    D+ L+K +   Q  EF  ++         +  IG    V GWG +   G   S  LQ+ S+ IVS+  C  +F       +IP   LCAG       +C+GDSGGPL  K+   +    GI+S+G+  C + N+P V T++S+   WI
Sbjct:  541 ETRIVGGKSAAFGRWPWQVSVRRTSFFGFSSTHRCGGALINENWIATAGHCV--------DDLLISQIRIRVGEYDFSHVQEQLPYIERGVAKKVVHPKYSF-LTY--EYDLALVKLE---QPLEFAPHVSPICLPETDSLLIGMNATVTGWGRLSEGGTLPS-VLQEVSVPIVSNDNCKSMFMRAGRQEFIPDIFLCAGYETGGQDSCQGDSGGPLQAKSQDGRFFLAGIISWGIG-CAEANLPGVCTRISKFTPWI 782          
BLAST of Trypsin-like serine protease vs. Ensembl Fly
Match: Sb (gene:FBgn0003319 transcript:FBtr0083250)

HSP 1 Score: 108.612 bits (270), Expect = 2.821e-26
Identity = 81/258 (31.40%), Postives = 125/258 (48.45%), Query Frame = 3
Query:   57 EWKVINGTQSILHEYPQMVSIR------LLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQ--VTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEF-QYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTEL---PYIPS-NLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            E +++ G  +    +P  VS+R          H CGG L+NEN + TA HC+        + L   ++ I +GEYD   V  +    +  V K +VHP Y++ ++Y    D+ L+K +   Q  EF  ++         +  IG    V GWG +   G   S  LQ+ S+ IVS+  C  +F       +IP   LCAG       +C+GDSGGPL  K+   +    GI+S+G+  C + N+P V T++S+   WI
Sbjct:  541 ETRIVGGKSAAFGRWPWQVSVRRTSFFGFSSTHRCGGALINENWIATAGHCV--------DDLLISQIRIRVGEYDFSHVQEQLPYIERGVAKKVVHPKYSF-LTY--EYDLALVKLE---QPLEFAPHVSPICLPETDSLLIGMNATVTGWGRLSEGGTLPS-VLQEVSVPIVSNDNCKSMFMRAGRQEFIPDIFLCAGYETGGQDSCQGDSGGPLQAKSQDGRFFLAGIISWGIG-CAEANLPGVCTRISKFTPWI 782          
BLAST of Trypsin-like serine protease vs. Ensembl Zebrafish
Match: si:ch73-182e20.4 (si:ch73-182e20.4 [Source:ZFIN;Acc:ZDB-GENE-131127-155])

HSP 1 Score: 117.087 bits (292), Expect = 7.082e-31
Identity = 85/261 (32.57%), Postives = 124/261 (47.51%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDY---TYEMSYLNGNDVGLIKFDGKVQNNEF-QYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSR-------------FLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWIT 794
            +++ G  S    +P MVS+R  G HICGG+L+N   VLTAAHC+          L    + + LG++        E    V+  + HP Y   TY+      ND+ L++    V  +++ + +   + Q  S +  G R    GWG I  SG+G  R              LQ+  L + S+ +C  I       P+ +CAG+     A   GDSGGPL  K   +  +Q G+VS+G   C QPN+P VF +VSE   WIT
Sbjct:   15 RIVGGLNSTEGAWPWMVSLRYYGNHICGGSLINNEWVLTAAHCV---------NLTRSNMLVYLGKWRRYAADVNEITRTVSNIIPHPSYNSTTYD------NDIALLQLSSTVHYSDYIKPVCLADEQ--SNFPPGTRSWATGWGRIGVSGKGGIRGRTTVSVPLPPPGILQEVKLKVYSNADCNSICHGR-INPNMICAGTRSGGKATFSGDSGGPLVSKQ-CSVWVQAGVVSHGYG-CAQPNLPEVFIRVSEYKQWIT 255          
BLAST of Trypsin-like serine protease vs. Ensembl Zebrafish
Match: si:dkeyp-93a5.2 (si:dkeyp-93a5.2 [Source:ZFIN;Acc:ZDB-GENE-131127-18])

HSP 1 Score: 114.39 bits (285), Expect = 1.599e-29
Identity = 85/257 (33.07%), Postives = 118/257 (45.91%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRG--SSR-----------FLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWIT 794
            +++ G  +    +P MVS+R  G H CGG+L+N   VLTAAHCI   T           + + LG++        E    V   + HP Y    S    ND+ L++    VQ   +        QN S +  G R  V GWG I  SG G  S R            LQ+  L + S+ +C+    + P  P+ +CAG+         GDSGGPL  K   +  +Q G+VS+G   C QP +P VF +VSE   WIT
Sbjct:   34 RIVGGVNATEGSWPWMVSLRKSGVHFCGGSLINNQWVLTAAHCISGKT--------TSSMHVYLGKWRRYETDQNEITRTVIDIIPHPSYNNRTS---DNDIALLQLSATVQYTVYIKPICLADQN-SNFPRGTRSWVTGWGRIGVSGTGGISGRTTVSVPLPAPGILQEVELQVYSNEKCSK-RCQGPITPNMICAGTRSGGKGTFYGDSGGPLMSKQ-CSVWVQAGVVSHGYG-CAQPKIPGVFIRVSEYKQWIT 275          
BLAST of Trypsin-like serine protease vs. Ensembl Zebrafish
Match: zgc:123295 (zgc:123295 [Source:ZFIN;Acc:ZDB-GENE-051127-11])

HSP 1 Score: 112.464 bits (280), Expect = 9.396e-29
Identity = 84/253 (33.20%), Postives = 130/253 (51.38%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIR--LLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLV----HPDYTYEMSYLNGNDVGLIKFDGKVQNNEF-QYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNL-CAG-SGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWIT 794
            K++ G  +    +P  VS++    G H CGG+L+N++ VL+AAHC         ++L   KV++      L+  SG    +Q+TK +V    HP+Y       N ND+ L+K D  V  N++ + +      N  TY  G    V GWG +  +       LQ+  + IVS+ +C   +     I SN+ CAG        +C+GDSGGP+  +NG ++ IQ GIVS+G   C +P  P V+ +VS+  +WIT
Sbjct:   35 KIVGGQNAGAGSWPWQVSLQSPTYGGHFCGGSLINKDWVLSAAHCF-------QDSLGTVKVKL-----GLQSQSGSNP-YQITKTVVQVINHPNYNNPS---NDNDIALVKLDSSVTFNDYIEPVCLAAAGN--TYAAGTLSWVTGWGKLSSAANQIPDILQEVEIPIVSNSDCKRAYPG--EITSNMICAGLLDQGGKDSCQGDSGGPMVSRNG-SQWIQSGIVSFG-RGCAEPGYPGVYARVSQYQDWIT 265          
BLAST of Trypsin-like serine protease vs. Ensembl Zebrafish
Match: zgc:112285 (zgc:112285 [Source:ZFIN;Acc:ZDB-GENE-050913-132])

HSP 1 Score: 111.694 bits (278), Expect = 1.993e-28
Identity = 80/254 (31.50%), Postives = 120/254 (47.24%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGE------HICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSS--RFLQKASLNIVSSYECAYI-FTELPYIPSNLCAG--SGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            ++++G ++  H +P  VS+++         H+CGGTL+++N VLTAAHC       K E    ++  IVLG++ L+R    E+ F V +   H  + Y        D+ L+K    +Q + F        + I+    G  C V GWG         S    L +A L I+    C    F       S +CAG      + AAC+GDSGGPL C+ G ++    GIVS+G   C   N P+VFT+ +    WI
Sbjct:   58 RIVSGNEARPHSWPWQVSLQVRPRGSKHYVHVCGGTLIHKNWVLTAAHC---FQKGKAEDASSWR--IVLGKHQLKRSETAERFFPVKRIYRHEHFRYPAHSELDYDIALVKAATDIQPSNFIRYACLPRKQIN-LNPGHYCWVTGWGDTRGGKENVSLAEALNQARLPIIDYKTCRQKKFWGDRVRDSMICAGFRDTEGTPAACQGDSGGPLLCQVGRDRWEVHGIVSFGPIGCTVENKPSVFTRTAAYIPWI 305          
BLAST of Trypsin-like serine protease vs. Ensembl Zebrafish
Match: si:dkey-21e2.13 (si:dkey-21e2.13 [Source:ZFIN;Acc:ZDB-GENE-050208-379])

HSP 1 Score: 110.153 bits (274), Expect = 2.246e-28
Identity = 83/252 (32.94%), Postives = 129/252 (51.19%), Query Frame = 3
Query:   45 TVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQ-NNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEP--CGQPNVPTVFTKVSEVANWI 791
            T +A   +++GT++  H  P MVS++    HICGG L+++  VLTAAHC      WK + L      +V+G +DL R++     F+VT  + HP+  ++++ L  ND+ L+K + KV  +N    I   +Y      +    C V GWG +   G    R L++A   IV+  EC   + +  Y+ S +    G   T  CKGDSGGPL C      +  VGI S+  +P  C    +P  +T++S   +WI
Sbjct:   27 TFTARVGIVDGTEAEPHSRPYMVSLQKKSRHICGGFLIHQQFVLTAAHC------WKGDPL-----TVVVGAHDL-RMTDSSNSFKVTSYVQHPN--FDLNTLK-NDIMLLKLNKKVTLSNNVGLISLPKYGEDVKADTA--CSVAGWGRLSRKGPKPDR-LREAETVIVNDAECQRRWRQT-YVASEMICVYGPGGT--CKGDSGGPLVC-----GDTAVGITSFS-DPYLCNSRLLPNTYTRISAYLSWI 251          
BLAST of Trypsin-like serine protease vs. Ensembl Xenopus
Match: trim36 (tripartite motif containing 36 [Source:Xenbase;Acc:XB-GENE-1017281])

HSP 1 Score: 129.028 bits (323), Expect = 4.865e-33
Identity = 80/243 (32.92%), Postives = 122/243 (50.21%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            +++ GTQ+   E+P   S+++ GEHICGGTL+ +  +LTAAHC  P +    E    Y     LG+  L R + +E  F+V + ++HP Y  +    +  DV L+  D  V              +   +  G  C V GWGS+  +G  +S  LQK  + +V+   C  ++      P  LCAG    S  AC+GDSG PL CK    +  Q G+VS+G   CG P    V+++++ +  WI
Sbjct:  522 RLVGGTQAQEGEWPWQASLQVRGEHICGGTLVADQWILTAAHCFTPESYASPEVWTVY-----LGKVRLSRSTQKELAFKVIRLVIHPFYDEDS---HDYDVALVLLDHLVPLTSPHVQPICLPSSTHHFPTGSSCWVTGWGSVKENG-PTSDVLQKVDIQLVAQDICTELY-RYQISPRMLCAGYRDGSKDACQGDSGSPLVCKTASGRWFQAGLVSWGAG-CGIPRYFGVYSRITRLVQWI 753          
BLAST of Trypsin-like serine protease vs. Ensembl Xenopus
Match: trim36 (tripartite motif containing 36 [Source:Xenbase;Acc:XB-GENE-1017281])

HSP 1 Score: 127.487 bits (319), Expect = 7.922e-33
Identity = 80/243 (32.92%), Postives = 122/243 (50.21%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            +++ GTQ+   E+P   S+++ GEHICGGTL+ +  +LTAAHC  P +    E    Y     LG+  L R + +E  F+V + ++HP Y  +    +  DV L+  D  V              +   +  G  C V GWGS+  +G  +S  LQK  + +V+   C  ++      P  LCAG    S  AC+GDSG PL CK    +  Q G+VS+G   CG P    V+++++ +  WI
Sbjct:  333 RLVGGTQAQEGEWPWQASLQVRGEHICGGTLVADQWILTAAHCFTPESYASPEVWTVY-----LGKVRLSRSTQKELAFKVIRLVIHPFYDEDS---HDYDVALVLLDHLVPLTSPHVQPICLPSSTHHFPTGSSCWVTGWGSVKENGP-TSDVLQKVDIQLVAQDICTELY-RYQISPRMLCAGYRDGSKDACQGDSGSPLVCKTASGRWFQAGLVSWGAG-CGIPRYFGVYSRITRLVQWI 564          
BLAST of Trypsin-like serine protease vs. Ensembl Xenopus
Match: ENSXETT00000015569.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:9:2816071:2829750:1 gene:ENSXETG00000010028.1 transcript:ENSXETT00000015569.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 119.013 bits (297), Expect = 7.479e-31
Identity = 81/258 (31.40%), Postives = 133/258 (51.55%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVE---IVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRF-LQKASLNIVSSYECAYIF---------TELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWITR 797
            +++ G  ++  E+P  VSI     HICGG+L++ N V++AAHC            + YK+E   ++LG + L  ++ +  I +V + + +P YT E S     D+ +++ +  V  + +         N   +  G+ C V GWG+I      S  + LQ+  + +V++  C  ++         T+L +    +CAG       AC+GDSGGPL CK+G N     GIVS+G + C QPN P V+TKVS  ++WI +
Sbjct:   35 RIVGGNNAVFGEWPWQVSIVYQNSHICGGSLVSSNWVVSAAHCF----------PRSYKIENMQVLLGCFALMNLTSDAVIIRVKRVITYPLYTGEGS---SGDIAMVEMESPVTYSSYILPICIPLTN-EDFPSGKMCWVTGWGNIQSDVSLSPPYPLQEVEVPLVNASSCDTMYHYNSDLNPATQLVH-DDMICAGYPEGQKDACQGDSGGPLACKSG-NYWFLTGIVSWG-DGCAQPNRPGVYTKVSSFSSWINQ 275          
BLAST of Trypsin-like serine protease vs. Ensembl Xenopus
Match: pcdh8l (protocadherin-8-like [Source:Xenbase;Acc:XB-GENE-11536541])

HSP 1 Score: 118.242 bits (295), Expect = 1.116e-30
Identity = 85/253 (33.60%), Postives = 127/253 (50.20%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNL-VHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSI-DPSGRGSSRFLQKASLNIVSSYECAYIF-TELPYIPS-------NLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            +++ GT S   E+P  +S+   G  +CGG+LL ++ VLTAAHC         +++   K    LG Y L  +  +  + +  KN+ VHPDY YE S     D+ LI+ +  +             Q++    +G  C V GWG+I + +     + LQKA + +++   C  ++ + L Y PS        +CAG       AC+GDSGGPL C N  N  +Q GIVS+GL  C +PN P V+T V     WI
Sbjct:   25 RIVGGTDSEEGEWPWQISLEFEGGFLCGGSLLTDSWVLTAAHC--------FDSMNVSKYTAYLGVYQLSDL--DNAVLRGVKNITVHPDYMYEGS---SGDIALIELEEPIVFTPSIQPVCLPSQDVP-LPMGTMCWVTGWGNIKENTPLEDPQTLQKAEVGLINRTSCEAMYQSSLGYRPSIHLIQDDMICAGYKQGKIDACQGDSGGPLVC-NTSNTWLQFGIVSWGLG-CAEPNQPGVYTNVQYYLTWI 261          
BLAST of Trypsin-like serine protease vs. Ensembl Xenopus
Match: ENSXETT00000030871.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:9:2823629:2829750:1 gene:ENSXETG00000010028.1 transcript:ENSXETT00000030871.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 118.242 bits (295), Expect = 1.319e-30
Identity = 81/258 (31.40%), Postives = 133/258 (51.55%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVE---IVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRF-LQKASLNIVSSYECAYIF---------TELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWITR 797
            +++ G  ++  E+P  VSI     HICGG+L++ N V++AAHC            + YK+E   ++LG + L  ++ +  I +V + + +P YT E S     D+ +++ +  V  + +         N   +  G+ C V GWG+I      S  + LQ+  + +V++  C  ++         T+L +    +CAG       AC+GDSGGPL CK+G N     GIVS+G + C QPN P V+TKVS  ++WI +
Sbjct:   36 RIVGGNNAVFGEWPWQVSIVYQNSHICGGSLVSSNWVVSAAHCF----------PRSYKIENMQVLLGCFALMNLTSDAVIIRVKRVITYPLYTGEGS---SGDIAMVEMESPVTYSSYILPICIPLTN-EDFPSGKMCWVTGWGNIQSDVSLSPPYPLQEVEVPLVNASSCDTMYHYNSDLNPATQLVH-DDMICAGYPEGQKDACQGDSGGPLACKSG-NYWFLTGIVSWG-DGCAQPNRPGVYTKVSSFSSWINQ 276          
BLAST of Trypsin-like serine protease vs. Ensembl Mouse
Match: Klk9 (kallikrein related-peptidase 9 [Source:MGI Symbol;Acc:MGI:1921082])

HSP 1 Score: 119.783 bits (299), Expect = 5.025e-32
Identity = 79/261 (30.27%), Postives = 126/261 (48.28%), Query Frame = 3
Query:   18 LIVFLIFISTVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGN-DVGLIKFDGKVQ-NNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSN-LCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            L++F +      A+ + +   + + +  P    +  L   +CG TL+N+  +LTAAHC            ++  + + LGE+ L R  G E++  VT    HP +  ++S  + N D+ LI+   KV+     Q +  TE    S   +G +C + GWGS+  S       LQ A+++I+ +  C + +    +I    LCAG       +C+GDSGGPL C     +    GIVS G EPC +P  P V+T V +   WI
Sbjct:    7 LVLFSLLAGHCGADTRAVGARECVRNSQPWQAGLFYLTRQLCGATLINDQWLLTAAHC------------RKPYLWVRLGEHHLWRWEGPEQLLLVTDFFPHPGFNPDLSANDHNDDIMLIRLPRKVRLTPAVQPLNLTE----SRPPVGTQCLISGWGSVSSSKLQYPMTLQCANISILDNKLCRWAYPG--HISEKMLCAGLWEGGRGSCQGDSGGPLVC-----EGTLAGIVSGGSEPCSRPRRPAVYTNVFDYLEWI 244          
BLAST of Trypsin-like serine protease vs. Ensembl Mouse
Match: Klk13 (kallikrein related-peptidase 13 [Source:MGI Symbol;Acc:MGI:3615275])

HSP 1 Score: 120.168 bits (300), Expect = 5.963e-32
Identity = 83/274 (30.29%), Postives = 135/274 (49.27%), Query Frame = 3
Query:   15 ILIVFLIFISTVSAEW-KVINGTQS-----------ILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLN-GNDVGLIKFDGKVQ-NNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSN-LCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            I  + L     +S ++ K++NGT             + H  P   ++ + G  +CGG L++   VLTAAHC            ++    + LG++ L RV   E+  +V +++ HP+Y    ++LN  +D+ L++    VQ ++  + +K +    + T   G  C V GWG+         + LQ A++ + S  EC  ++     I +N LCAG+      +C+GDSGGPL C          GI+S+G  PCGQPN P V+T+VS+   WI
Sbjct:    8 IACLTLALSEGISRDYPKILNGTNGTSGFLPGGYTCLPHSQPWQAALLIRGRLLCGGVLVHPKWVLTAAHC------------RKDGYTVHLGKHALGRVENGEQAMEVVRSIPHPEYQVTPTHLNHDHDIMLLELKSPVQLSSHVRTLKLSADDCLPT---GTCCRVSGWGTTTSPQVNYPKTLQCANIELRSDEECRQVYPG--KITANMLCAGTKEGGKDSCEGDSGGPLICNGKL-----YGIISWGDFPCGQPNRPGVYTRVSKYLRWI 259          
BLAST of Trypsin-like serine protease vs. Ensembl Mouse
Match: Gzmm (granzyme M (lymphocyte met-ase 1) [Source:MGI Symbol;Acc:MGI:99549])

HSP 1 Score: 114.775 bits (286), Expect = 5.613e-30
Identity = 80/249 (32.13%), Postives = 121/249 (48.59%), Query Frame = 3
Query:   57 EWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYEC-AYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQV-GIVSYGLEPCGQPNVPTVFTKVSEVANWITR 797
            E ++I G +++ H  P M S++    H+CGG L++   VLTAAHC+        E L+  K  +VLG ++L  +      F + + + HP Y ++      ND+ L+K D +VQ ++         +  S    G  C   GWG +   G   +R LQ+  L ++ +  C    F     I S LC  +G  S A CKGDSGGPL C  G     QV GI+S+  + C     P V T V+  ++WI +
Sbjct:   24 ETQIIGGREAVPHSRPYMASLQKAKSHVCGGVLVHRKWVLTAAHCL-------SEPLQNLK--LVLGLHNLHDLQDPGLTFYIREAIKHPGYNHKYE----NDLALLKLDRRVQPSKNVKPLALPRKPRSKPAEGTWCSTAGWG-MTHQGGPRARALQELDLRVLDTQMCNNSRFWNGVLIDSMLCLKAGSKSQAPCKGDSGGPLVCGKG-----QVDGILSFSSKTCTDIFKPPVATAVAPYSSWIRK 253          
BLAST of Trypsin-like serine protease vs. Ensembl Mouse
Match: Klk8 (kallikrein related-peptidase 8 [Source:MGI Symbol;Acc:MGI:1343327])

HSP 1 Score: 113.235 bits (282), Expect = 1.562e-29
Identity = 75/264 (28.41%), Postives = 122/264 (46.21%), Query Frame = 3
Query:   21 IVFLIFIS-----TVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWITR 797
            I+ L+F+      T +   K++ G + I H  P   ++      ICGG L+ +  VLTAAHC            K+ K  + LG++ L+     E+  QV +++ HP Y       + +D+ LI+       N    +K  +  N+   ++G++C + GWG++          L  A + I S  +C   +         +CAGS +     C+GDSGGPL C       +  GI S+G +PCG+P  P V+TK+     WI +
Sbjct:   13 ILLLLFMGAWAGLTRAQGSKILEGRECIPHSQPWQAALFQGERLICGGVLVGDRWVLTAAHC------------KKQKYSVRLGDHSLQSRDQPEQEIQVAQSIQHPCYNNSNPEDHSHDIMLIRLQNSA--NLGDKVKPVQLANLCP-KVGQKCIISGWGTVTSPQENFPNTLNCAEVKIYSQNKCERAYPGK-ITEGMVCAGSSN-GADTCQGDSGGPLVCDG-----MLQGITSWGSDPCGKPEKPGVYTKICRYTTWIKK 254          
BLAST of Trypsin-like serine protease vs. Ensembl Mouse
Match: Prss38 (protease, serine 38 [Source:MGI Symbol;Acc:MGI:2685095])

HSP 1 Score: 114.39 bits (285), Expect = 2.262e-29
Identity = 78/244 (31.97%), Postives = 121/244 (49.59%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYEC-AYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            K++ G  +   ++P  VS+   G HICGG++L+   VL+AAHC       KLET      +I +G  +LE+ +   + F++ + ++HP  T++M +  G DV L++    +  ++F         ++  Y I   C   GWG I P G   +  L+ A L ++  ++C          +P  LCA         C+GDSG PL CK      +Q+GIVS+G   C QP  P VF  VS   +WI
Sbjct:   55 KLLGGEFARDRKWPWQVSLHYSGFHICGGSILSAYWVLSAAHCF--DRGKKLET-----YDIYVGITNLEKANRHTQWFEIYQVIIHP--TFQMYHPIGGDVALVQLKSAIVFSDFVLPICLPPSDL--YLINLSCWTTGWGMISPQGETGNELLE-AQLPLIPRFQCQLLYGLSSYLLPEMLCAADIKTMKNVCEGDSGSPLVCKQN-QTWLQIGIVSWG-RGCAQPLYPGVFANVSYFLSWI 284          
BLAST of Trypsin-like serine protease vs. UniProt/SwissProt
Match: sp|Q9UKR3|KLK13_HUMAN (Kallikrein-13 OS=Homo sapiens OX=9606 GN=KLK13 PE=2 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 1.918e-31
Identity = 85/250 (34.00%), Postives = 125/250 (50.00%), Query Frame = 3
Query:   72 NGTQSIL--------HEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLN-GNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSN-LCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            NGT   L        H  P   ++ + G  +CGG L++   VLTAAHC+        E LK Y     LG++ L RV   E++ +V  ++ HP+Y    ++LN  +D+ L++    VQ     YI+     + +    G  C V GWG+         + LQ A++ + S  EC  ++     I  N LCAG+      +C+GDSGGPL C    N+ +  GIVS+G  PCGQP+ P V+T+VS    WI
Sbjct:   30 NGTSGFLPGGYTCFPHSQPWQAALLVQGRLLCGGVLVHPKWVLTAAHCL-------KEGLKVY-----LGKHALGRVEAGEQVREVVHSIPHPEYRRSPTHLNHDHDIMLLELQSPVQLTG--YIQTLPLSHNNRLTPGTTCRVSGWGTTTSPQVNYPKTLQCANIQLRSDEECRQVYPG--KITDNMLCAGTKEGGKDSCEGDSGGPLVC----NRTL-YGIVSWGDFPCGQPDRPGVYTRVSRYVLWI 258          
BLAST of Trypsin-like serine protease vs. UniProt/SwissProt
Match: sp|Q6DT45|KLK3_MACFA (Prostate-specific antigen OS=Macaca fascicularis OX=9541 GN=KLK3 PE=2 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 2.629e-30
Identity = 84/279 (30.11%), Postives = 129/279 (46.24%), Query Frame = 3
Query:   15 ILIVFLIFISTVSAEW---------KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTY-----------EIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            +L+VFL    T+S  W         +++ G +   H  P  V +   G  +CGG L++   VLTAAHCI           + + V I+LG ++        ++FQV+ +  HP   Y MS L    +G     G   +++   ++ +E   I+             E+G  C   GWGSI+P    + + LQ   L+I+S+  CA + ++       LCAGS     + C GDSGGPL C       +  GI S+G +PC  P  P+++TKV     WI
Sbjct:    3 VLVVFL----TLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASHGRAVCGGVLVHPQWVLTAAHCI-----------RSHSV-ILLGRHNPYYPEDTGQVFQVSHSFPHP--LYNMSLLKNRYLG----PGDDSSHDLMLLRLSEPAEITDAVQVLDLPTWEPELGTTCYASGWGSIEPEEHLTPKKLQCVDLHIISNDVCAQVHSQ-KVTKFMLCAGSWMGGKSTCSGDSGGPLVC-----DGVLQGITSWGSQPCALPRRPSLYTKVVRYRKWI 253          
BLAST of Trypsin-like serine protease vs. UniProt/SwissProt
Match: sp|P09582|ESTA_CANLF (Arginine esterase OS=Canis lupus familiaris OX=9615 PE=1 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 3.561e-30
Identity = 77/252 (30.56%), Postives = 116/252 (46.03%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYL-------NGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYE--IGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            ++I G + + +  P  V++   GE  CGG L+N   VLTAAHC                 E+ LG ++L     E ++ QV K+ +HP Y  ++            +D+ L+  +        +  K     ++   E  +G  C V GWGS DP        LQ   L ++S+ +CA ++T+       LCAG        CKGDSGGPL C         VGI S+G  PCG+P +P+++T+V     WI
Sbjct:   24 RIIGGRECLKNSQPWQVAVYHNGEFACGGVLVNPEWVLTAAHC------------ANSNCEVWLGRHNLSESEDEGQLVQVRKSFIHPLYKTKVPRAVIRPGEDRSHDLMLLHLE-----EPAKITKAVRVMDLPKKEPPLGSTCYVSGWGSTDPETIFHPGSLQCVDLKLLSNNQCAKVYTQ-KVTKFMLCAGVLEGKKDTCKGDSGGPLICDGEL-----VGITSWGATPCGKPQMPSLYTRVMPHLMWI 252          
BLAST of Trypsin-like serine protease vs. UniProt/SwissProt
Match: sp|P33619|KLK3_MACMU (Prostate-specific antigen OS=Macaca mulatta OX=9544 GN=KLK3 PE=2 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 6.161e-30
Identity = 83/279 (29.75%), Postives = 126/279 (45.16%), Query Frame = 3
Query:   15 ILIVFLIFISTVSAEW---------KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTY-----------EIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            +L+VFL    T+S  W         +++ G +   H  P  V +   G  +CGG L++   VLTAAHCI                 I+LG ++        ++FQV+ +  HP   Y MS L    +G     G   +++   ++ +E   I+             E+G  C   GWGSI+P    + + LQ   L+I+S+  CA + ++       LCAGS     + C GDSGGPL C       +  GI S+G +PC  P  P+++TKV     WI
Sbjct:    3 VLVVFL----TLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCI------------RSNSVILLGRHNPYYPEDTGQVFQVSHSFPHP--LYNMSLLKNRYLG----PGDDSSHDLMLLRLSEPAEITDAVQVLDLPTWEPELGTTCYASGWGSIEPEEHLTPKKLQCVDLHIISNDVCAQVHSQ-KVTKFMLCAGSWMGGKSTCSGDSGGPLVC-----DGVLQGITSWGSQPCALPRRPSLYTKVVRYRKWI 253          
BLAST of Trypsin-like serine protease vs. UniProt/SwissProt
Match: sp|A1L453|PRS38_HUMAN (Serine protease 38 OS=Homo sapiens OX=9606 GN=PRSS38 PE=2 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 8.146e-30
Identity = 80/251 (31.87%), Postives = 130/251 (51.79%), Query Frame = 3
Query:   51 SAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNE--FQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYI-PSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKN-IQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            S E K++ G  +   ++P  VS+   G H+CGG++LNE  VL+AAHC       + + +K Y  ++ +G  +L       + ++V + ++HP  TYEM +  G DV L++   ++  +E        T   N+++      C   GWG +   G  S   LQ+  L ++    C  ++  + YI P  LCAG    +   C+GDSGGPL C+  +N++ +Q+GIVS+G   C  P  P V+  VS  + WI
Sbjct:   55 SMEGKILGGVPAPERKWPWQVSVHYAGLHVCGGSILNEYWVLSAAHCF-----HRDKNIKIY--DMYVGLVNLRVAGNHTQWYEVNRVILHP--TYEMYHPIGGDVALVQLKTRIVFSESVLPVCLATPEVNLTS----ANCWATGWGLVSKQGETSDE-LQEMQLPLILEPWCHLLYGHMSYIMPDMLCAGDILNAKTVCEGDSGGPLVCE--FNRSWLQIGIVSWG-RGCSNPLYPGVYASVSYFSKWI 288          
BLAST of Trypsin-like serine protease vs. TrEMBL
Match: H2DRI7 (Trypsin-like serine protease OS=Dugesia japonica OX=6161 PE=2 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 3.299e-81
Identity = 115/197 (58.38%), Postives = 149/197 (75.63%), Query Frame = 3
Query:  201 MTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            M  W L T K Y++E+ L EYDL R+ G EKIFQVTK ++HP Y +E +YL GNDV LIKFDG + N ++ YI Y E    + ++ G+RCEVVGWGS+ P G+  SRFLQKA++NI+S+ EC  IF+ +  + SN+CAGSG LST++C+GDSGGPLFC +G   +IQVGIVSYG  PCGQPN+P+V+T+VSEVA WI
Sbjct:    1 MKSWNLNTFKYYQMEVYLAEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGFISNGQYDYIHYNELDVNAIFKPGDRCEVVGWGSMLPPGQDISRFLQKAAINIISNSECGRIFSNVADVRSNICAGSGRLSTSSCRGDSGGPLFCTDGKRHHIQVGIVSYGTVPCGQPNIPSVYTRVSEVAGWI 197          
BLAST of Trypsin-like serine protease vs. TrEMBL
Match: A0A1I8GT16 (Peptidase S1 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig010631g1 PE=3 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 4.477e-46
Identity = 89/265 (33.58%), Postives = 148/265 (55.85%), Query Frame = 3
Query:    9 LNILIVFLIFISTVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLE-RVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQN--ISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYN-KNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            +  L++F       + +  +I+G ++   ++P +VS++  G H CGGTL ++ TV+TAAHC +  +         + + +V GE DL    S   + ++V++ ++HPDY     Y+ G+D+ ++K            IK  ++++  +     G  C+V GWG+IDPSGR +        ++ +++ +CA +F  +      +CAG G  +T AC+GDSGGPL+C   YN K  QVGIVSYG  PCGQ  +PTV+T+V+    +I
Sbjct:    1 MKFLVLFACLSVANAYDRLIIDGVEASTGQHPDLVSLQRNGNHFCGGTLYDKRTVVTAAHCFYGNS-------GTFGLAVVAGENDLTLSNSANRRSYRVSRVIIHPDYRSSYHYVQGDDIAILKLSSDADLGTAT-IKAGDFEDAGVDIPPAGSSCKVAGWGAIDPSGRYAHTRKMIGGVSSITNNDCARVFNRVLGNTKQVCAGGG-TTTNACRGDSGGPLYCT--YNGKTRQVGIVSYGRVPCGQVGIPTVYTRVATYRRFI 254          
BLAST of Trypsin-like serine protease vs. TrEMBL
Match: A0A1I8JAW5 (Peptidase S1 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig007344g1 PE=3 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 1.117e-45
Identity = 89/265 (33.58%), Postives = 148/265 (55.85%), Query Frame = 3
Query:    9 LNILIVFLIFISTVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLE-RVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQN--ISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYN-KNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            +  L++F       + +  +I+G ++   ++P +VS++  G H CGGTL ++ TV+TAAHC +  +         + + +V GE DL    S   + ++V++ ++HPDY     Y+ G+D+ ++K            IK  ++++  +     G  C+V GWG+IDPSGR +        ++ +++ +CA +F  +      +CAG G  +T AC+GDSGGPL+C   YN K  QVGIVSYG  PCGQ  +PTV+T+V+    +I
Sbjct:    1 MKFLVLFACLSVANAYDRLIIDGVEASTGQHPDLVSLQRNGNHFCGGTLYDKRTVVTAAHCFYGNS-------GTFGLAVVAGENDLTLSNSANRRSYRVSRVIIHPDYRSSYHYVQGDDIAILKLSSDADLGTAT-IKAGDFEDAGVDIPPEGSSCKVAGWGAIDPSGRYAHTRKMIGGVSSITNNDCARVFNRVLGNTKQVCAG-GDTTTNACRGDSGGPLYCT--YNGKTRQVGIVSYGRVPCGQVGIPTVYTRVATYRRFI 254          
BLAST of Trypsin-like serine protease vs. TrEMBL
Match: B0X1E9 (Chymotrypsin A OS=Culex quinquefasciatus OX=7176 GN=6046245 PE=3 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 2.953e-36
Identity = 90/244 (36.89%), Postives = 125/244 (51.23%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRG-SSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            K+  G  S  HE+P  +S++L G H CGG+L+++  VLTA HC   +       L    VE+V GE+D    S   +   V +  VH DY   M  +  NDV +++ D   + NEF  +     +N+        C + GWGS+  +        LQKA L I+   +C  I+   P   SN+CAG+    T  C GDSGGPL  +   +K  QVGIVS+G  PCG PN P VF +VS   +WI
Sbjct:   25 KIAGGVPSSEHEFPYQISLQLDGRHFCGGSLISDRFVLTAGHCTGAL-------LSGLTVEVVAGEHDFSAPSEHAQRRVVDEFWVHEDYVVGM--VAPNDVAVLRVDRPYELNEFVGLVQLPGKNVLH---DGTCVISGWGSVSFNAYPIYPDVLQKAYLPIMDQEQCVKIWEGGPVADSNVCAGTVEGGTGVCSGDSGGPLVMEGPEHKE-QVGIVSWGGVPCGGPNNPGVFVRVSYFIDWI 255          
BLAST of Trypsin-like serine protease vs. TrEMBL
Match: A0A1I8JHD8 (Peptidase S1 domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 9.661e-36
Identity = 82/266 (30.83%), Postives = 134/266 (50.38%), Query Frame = 3
Query:    9 LNILIVFLIFISTVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLE-RVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQN--ISTYEIGERCEVVGWGSIDPSGR-GSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYN-KNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            +  L++F       + +  +I+G ++   ++P +VS++  G H CGGTL ++ TV+TAAHC +  +         + + +V GE DL    S   + ++V++ ++HPDY     Y+ G+D+ ++K            IK  ++++  +     G  C+V GWG+IDPSGR   +R +        ++                           AC+GDSGGPL+C   YN K  QVGIVSYG  PCGQ  +PTV+T+V+    +I
Sbjct:    1 MKFLVLFACLSVANAYDRLIIDGVEASTGQHPDLVSLQRNGNHFCGGTLYDKRTVVTAAHCFYGNS-------GTFGLAVVAGENDLTLSNSANRRSYRVSRVIIHPDYRSSYHYVQGDDIAILKLSSDADLGTAT-IKAGDFEDAGVDIPPAGSSCKVAGWGAIDPSGRYAHTRKMIGGVRGGGTTTN-------------------------ACRGDSGGPLYCT--YNGKTRQVGIVSYGRVPCGQVGIPTVYTRVATYRRFI 231          
BLAST of Trypsin-like serine protease vs. Ensembl Cavefish
Match: ENSAMXT00000043830.1 (elastase-1-like [Source:NCBI gene;Acc:103036513])

HSP 1 Score: 116.701 bits (291), Expect = 8.383e-31
Identity = 78/222 (35.14%), Postives = 116/222 (52.25%), Query Frame = 3
Query:  135 HICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNV---PTVFTKVSEVANWI 791
            HICGG+L++   V+TAAHCI  M       L+ Y+V  VLG+YDL +  G E+   V++ ++HP +T ++  +NGND+ L+K    V  N +  I    Y +  T   G  C + GWG +  +G  +   LQ+A + +V    CA        +   +    G   T+ C+GDSGGPL C       +  G+VS+G  P    N+   PTVFT+VS   +WI
Sbjct:   62 HICGGSLISSLYVMTAAHCILDM------QLRFYRV--VLGDYDLYKYEGGEQPRSVSRIIMHPGWTGDL--VNGNDIALLKLSSPVYGNSYTEIANFPYPD-QTLPNGFTCYITGWGLL-ATGGTTPDILQEAPIPVVEHSVCASPGWWSTAVKKTMVCAGGDGKTSGCQGDSGGPLNCYTDGAWRVH-GVVSFG--PASNCNIYTKPTVFTRVSSFMDWI 268          
BLAST of Trypsin-like serine protease vs. Ensembl Cavefish
Match: ENSAMXT00000048530.1 (pep primary_assembly:Astyanax_mexicanus-2.0:13:20283923:20290642:1 gene:ENSAMXG00000040955.1 transcript:ENSAMXT00000048530.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 116.316 bits (290), Expect = 1.154e-30
Identity = 78/251 (31.08%), Postives = 123/251 (49.00%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSG--RGSSRFLQKASLNIVSSYEC----AYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWITR 797
            +V+ G  ++  E+P   S+++ G HICGG L+    V++AAHC +   D +L +   + V   LG+  L   S  E+  +++   +H  Y  E    +  D+ L++ D  V              +    E G  C V GWG++   G  RG S  LQK  + +VS   C     YI T     P  LCAG       AC+GDSGGPL C+    +    G+VS+G   CG+P+   V+T+++ +++WI +
Sbjct:   28 RVVGGADAVEGEWPWQASLQVSGRHICGGALIASQWVVSAAHCFY---DDRLYSPSVWTV--YLGKLRLRGTSQTEEALRLSHIHIHQYYDDEN---HDYDLALLRLDRPVSAGTLAQPACLP-SHTHQLEPGLLCWVTGWGAMKEGGEKRGPSNVLQKVDVRLVSEEACVRSYGYIIT-----PRMLCAGYRSGGKDACQGDSGGPLVCQEPSGRWFLAGVVSWG-RGCGRPDYYGVYTRITRLSDWIKQ 263          
BLAST of Trypsin-like serine protease vs. Ensembl Cavefish
Match: ENSAMXT00000001125.2 (mast cell protease 4-like [Source:NCBI gene;Acc:103031537])

HSP 1 Score: 114.39 bits (285), Expect = 3.815e-30
Identity = 81/245 (33.06%), Postives = 123/245 (50.20%), Query Frame = 3
Query:   66 VINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNE-FQYIKY-TEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYG-LEPCGQPNVPTVFTKVSEVANWI 791
            ++NGT++  H  P MVS+++  +H CGG L++E+ V+TAAHC      WK    K+     V+G +D+   +     F V K  VHP Y  + +Y    D+ L+K  G V  +E  ++I    + ++I    +   C V GWG       GS   L+  +  I+    C  ++ + P  P  +CA         C GDSGGPL C      ++ VGIVS+G    C +P  P V+TK+SE+  WI
Sbjct:   26 IVNGTEAKPHSRPYMVSVQIDEKHQCGGFLVSESFVMTAAHC------WK----KKVTFTAVVGAHDISNKNEGSIRFPVEKYAVHPQYQDDETY--DYDILLLKLKGTVIKSERVKWISIPAQKEDIPANSV---CSVAGWGRTGTKKSGSHCLLE-TNTKIMDKARCTSLW-DTPITPRMVCA---QHPGGPCWGDSGGPLVC-----DSVAVGIVSFGDANTCDKPIQPEVYTKISEILPWI 245          
BLAST of Trypsin-like serine protease vs. Ensembl Cavefish
Match: si:dkey-78l4.13 (si:dkey-78l4.13 [Source:ZFIN;Acc:ZDB-GENE-060503-459])

HSP 1 Score: 112.079 bits (279), Expect = 2.980e-29
Identity = 81/263 (30.80%), Postives = 132/263 (50.19%), Query Frame = 3
Query:   21 IVFLIFISTVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKY----TEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSY-GLEPCGQPNV-PTVFTKVSEVANWI 791
            I  L+F+   S +  +INGT++  H  P MVS++  G+H CGG L++E+ V+TAAHC      W    + E K+ +VLG ++L++ +       V    +HP Y  E      ND+ L++ +   + +  Q I +    T+ ++I   +  + C V GWG  + +G  S R ++   + ++ +  C   +         +CA  G      C+GDSGGPL C     K   VGIVS+   + C  P + P V+TK+S+   WI
Sbjct:    5 ITLLLFLHLASVDVGIINGTEAKPHSRPYMVSVQRNGKHTCGGFLVSEHFVMTAAHC------WNYSNIGE-KLTVVLGAHELQKSNSALGRVAVKFYHIHPMYDSERLL---NDIMLLQLNKTAKKS--QRISWIPIPTKDKDI---KAKQACSVAGWGRQETNGSVSKRLME-VDVRVMDNKLCQKYWRTDYSTSRMVCAEQGF-----CQGDSGGPLVC-----KGTAVGIVSFTDSKSCNSPKMLPNVYTKISKFLPWI 241          
BLAST of Trypsin-like serine protease vs. Ensembl Cavefish
Match: ENSAMXT00000039451.1 (trypsin-like [Source:NCBI gene;Acc:111192060])

HSP 1 Score: 112.849 bits (281), Expect = 5.471e-29
Identity = 82/251 (32.67%), Postives = 118/251 (47.01%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNI-STYEIGERCEVVGWGSI-------DPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            K++ G  ++   +P  VSI+ LG H CGG+L+N N +L+AAHC       +  TL   +V   LG+  L+          V + + HP Y   +S  + ND+ L++    V  ++  YI+        ST   G    V GWG I       DP      + LQ+  L IVS+  C   +       + +CAG       +C+GDSGGPL  K       Q GIVS+G   C  P +P V+ +VSE  NWI
Sbjct:   36 KIVGGNTAVSGSWPWQVSIQSLGSHFCGGSLINNNWILSAAHC---FQSSQTSTLAFLRVS--LGKQSLQTSELNSLTITVAQIINHPSY---VSSTHDNDIALLQLSSSVTFSD--YIQPVCLAAAGSTLGSGTLVWVTGWGDISSDVSLPDP------QNLQEVELPIVSNTACTSAYGSGSITSNMMCAGLSQGGKDSCQGDSGGPLVIKQSTGWT-QAGIVSFGYG-CALPKIPGVYARVSEYQNWI 268          
BLAST of Trypsin-like serine protease vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008289.1 (pep scaffold:Pmarinus_7.0:GL480450:20964:30734:1 gene:ENSPMAG00000007473.1 transcript:ENSPMAT00000008289.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 119.783 bits (299), Expect = 7.392e-33
Identity = 80/243 (32.92%), Postives = 115/243 (47.33%), Query Frame = 3
Query:   66 VINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSN-LCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            ++ G++   H  P   S+R  G HICG  L+  N V+T+AHC     + K          +VLG++ + +  G EK F V++ + H  +    +  N NDV L+K    V  N    I+    Q  S    G  C V GWG++  S    S  L+  ++ IVS+  C         I  N LCAG       AC+GDSGGPL C N        G+VS+GL  C +P    ++T VS + +WI
Sbjct:   26 IVGGSEVAPHAIPSQASLRYNGVHICGAALIRTNWVITSAHCYKAAQNLK----------VVLGDHSMSKADGTEKTFSVSRVVRHEKFN---NVTNDNDVMLVKLSANVALNA--NIQLVALQT-SALPPGTLCNVSGWGTLQESSNKGSDVLRAVTVPIVSNALCNSTLYYRGLITVNMLCAGFDQGGKDACQGDSGGPLICDNKLK-----GLVSWGLG-CARPRYFGIYTDVSRLISWI 246          
BLAST of Trypsin-like serine protease vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002946.1 (pep scaffold:Pmarinus_7.0:GL477988:107792:112981:-1 gene:ENSPMAG00000002665.1 transcript:ENSPMAT00000002946.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 102.064 bits (253), Expect = 5.222e-26
Identity = 71/248 (28.63%), Postives = 124/248 (50.00%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRL-LGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNE-FQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPS-NLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWITR 797
            +++ G +++   +P  VS++   G H CGG+L++   ++TAAHC           ++  +  ++LGE+D    S + +   + K + HP +  ++S+   NDV LIK     Q  +    +  +   +      G  C   GWG  +     +   LQ+ +L IV++  C  +F  +  I    +CAG    S+  C+ DSGGPL CK     N+ VGIVS+G   C   ++P V+T+V+ + +WI +
Sbjct:   35 RIVKGEEAMPGSWPWQVSLQNNTGFHFCGGSLIDPYWIVTAAHC----------KIRPSEHRVILGEHDRSSSSEKVQTMTIAKVINHPQWD-DVSF--NNDVALIKLPQPAQMTDGVSAVCLSSTSD--NVPAGLNCVTSGWGITNNFDSDTPPRLQQTALAIVATPTCQQLFASMNKITDVMICAGGAGASS--CRCDSGGPLVCKKNGAWNL-VGIVSWGSSVCST-SMPVVYTRVTAIRSWIDQ 263          
BLAST of Trypsin-like serine protease vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005493.1 (pep scaffold:Pmarinus_7.0:GL476632:243920:250733:-1 gene:ENSPMAG00000004970.1 transcript:ENSPMAT00000005493.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 100.908 bits (250), Expect = 1.077e-25
Identity = 71/248 (28.63%), Postives = 120/248 (48.39%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIR-LLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNE-FQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPS-NLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWITR 797
            +++NG +++   +P  VS++   G H CGG+L++   V+TAAHC           +   +  ++LGE+D    S + +   + K + HP +   MS    NDV LIK     Q  +    +  +   +   +  G  C   GWG  +     +   LQ+ +L IV++  C  +   +  I    +CAG       +C+ DSGGPL C+     N+ VGIVS+G   C    +P V+T+V+ + +WI +
Sbjct:   26 RIVNGEEAMPGSWPWQVSLQSKTGFHFCGGSLIDPYWVVTAAHC----------GISPSEHRVILGEHDRSSSSEKVQTMTIAKAINHPQWD-NMSI--NNDVALIKLSQPAQMTDGVSAVCLSSTSD--NFPPGLNCVTSGWGITNNFDSDTPPRLQQTALAIVATPTCQQLLANMNKITDVMICAGGA--GATSCRRDSGGPLVCEKDGAWNL-VGIVSWGNSSCST-RIPAVYTRVTAIRSWIDQ 254          
BLAST of Trypsin-like serine protease vs. Ensembl Sea Lamprey
Match: si:dkey-33m11.8 (si:dkey-33m11.8 [Source:ZFIN;Acc:ZDB-GENE-141215-49])

HSP 1 Score: 100.523 bits (249), Expect = 1.347e-25
Identity = 80/245 (32.65%), Postives = 109/245 (44.49%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKF-DGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRG-SSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            +++ G +   +  P   S++  G H CGGTL+N   VLTAAHC     D +           VLGE+ L  V G+E+ F V + + H  Y         ND+ L+K     V N   Q ++       +  E G  C V GWG++   G G     L+   L+  S   C   ++      +  CA        AC+GDSGGPL C    N     GIVS+G   C  PN P V+TKVS    WI
Sbjct:   21 RIVGGFEVARNSLPYQASLQQNGVHYCGGTLINAKWVLTAAHCEITSLDLR-----------VLGEHSLGNVEGKEQTFMVERAIRHAGYDPATV---DNDIMLLKLPRAAVLNAAVQPVQLARAG--ARLEDGTSCLVSGWGTV---GNGVMPDALRAVYLDTTSRGYCNDAYSGT-LTDNMFCAVYAAGGRDACQGDSGGPLICNGALN-----GIVSWGYG-CAYPNYPGVYTKVSNYITWI 239          
BLAST of Trypsin-like serine protease vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006511.1 (pep scaffold:Pmarinus_7.0:GL476457:197255:208031:1 gene:ENSPMAG00000005872.1 transcript:ENSPMAT00000006511.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 100.523 bits (249), Expect = 1.953e-25
Identity = 76/263 (28.90%), Postives = 129/263 (49.05%), Query Frame = 3
Query:   30 LIFISTVSAEWKVINGTQSILHEYPQMVSIRLLGE----HICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGER--CEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNL-CAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWITR 797
            L ++  VSA+ +++NG ++  H +P   S++        H+CGG+L+ +  V+TAAHCI        +  + Y+  +VLG+++L      +    +  ++VHP +    +     D+ L+K       +E   IK      +   E+ +   C   GWG +   G+  S  LQ+A L  V+   C+        + +NL CAG  H   A C GDSGGPL C+      +  GI S+G + C   + P+VFT+VS   +W+++
Sbjct:   18 LTYLWHVSAQNRIVNGVEAAPHTWPWQASLQAQTGTGWYHVCGGSLVQQQWVMTAAHCI--------QADRVYR--MVLGKHNLRINEPAQFESTIALSVVHPSWNNHPTR-GWFDIALMKLSSAAPLSE--QIKLAVLPRVGQ-ELWDNVTCMATGWGLLASDGKFPS-VLQEAKLPFVNYSTCSQQEWWWYGVNTNLICAGDDH---AVCNGDSGGPLSCQEPNGSWVVYGITSFGHKNCSVFHKPSVFTRVSAYTDWVSQ 262          
BLAST of Trypsin-like serine protease vs. Ensembl Nematostella
Match: EDO30908 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZF8])

HSP 1 Score: 119.398 bits (298), Expect = 1.760e-32
Identity = 78/243 (32.10%), Postives = 125/243 (51.44%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            +V+NG  +  H +P  +S+R+ G HICGG+L+    VLTAAHC+        +        +V+G + ++  S  ++ F++TK   H D++  MS+L  NDV L+K D  +Q ++         +N S  ++G +C + GWG +   G+  S  LQ+A L + S   C    + L  +       +G +    C+GDSGGP  C  G  + +  G VS+G   CG  +  TVF +VS   +WI
Sbjct:   10 RVVNGDNAAQHSWPWQISLRVNGRHICGGSLITPEWVLTAAHCV-------EDFPHPSGYTVVVGAHRIKERSAIQQEFRLTKLFKHKDFS--MSHLK-NDVALLKLDNPIQPSDKVNTVCLPERN-SRAQVGAQCFITGWGRLVGGGQ-PSEILQQAMLPVASHSACDRKNSYLVPVEEKSMICAGGMGMGGCQGDSGGPFVCNEG-GRWVLRGAVSWGQPMCGTDHY-TVFARVSSFIDWI 238          
BLAST of Trypsin-like serine protease vs. Ensembl Nematostella
Match: EDO39910 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8P7])

HSP 1 Score: 117.087 bits (292), Expect = 9.653e-32
Identity = 78/247 (31.58%), Postives = 126/247 (51.01%), Query Frame = 3
Query:   66 VINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEY-KVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWG-SIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAG-SGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWITR 797
            +I+G+ +  + +P MV I     H CGGTL++   V+TAAHC+  + D      K Y ++ I LGE+      G E+ F V + +VHP Y +E + +N ND+ LIK +   + N++  +     Q     + G+ C   GWG ++    +  S  L++  L +V+  EC   + ++P   + +C G  G  S + C  DSGGP+ CK+        G+VS+G   C  P   +V  KVS+   WI +
Sbjct:    1 MISGSDAQPNSWPWMVQINYNNGHHCGGTLVSPQWVVTAAHCVDHVKD-----PKNYNELAITLGEHKRSASEGTEQRFSVARIIVHPQY-FEPTAIN-NDIALIKLNKPARLNKYVNLACLPRQGEELSD-GKICYATGWGLTVGGDWKSQSDVLKQTPLPVVNRQECQTDYDDIPITTAMMCTGYGGRSSISTCNTDSGGPVVCKSKLGHWYLQGVVSFGARACA-PGHYSVNAKVSKFVTWINQ 238          
BLAST of Trypsin-like serine protease vs. Ensembl Nematostella
Match: EDO42420 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1T0])

HSP 1 Score: 112.079 bits (279), Expect = 1.048e-29
Identity = 83/260 (31.92%), Postives = 125/260 (48.08%), Query Frame = 3
Query:   27 FLIFIST----VSAEWKVINGTQSILHEYPQMVSIRL-LGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
             LI I++    VS   +++ GT +    +P    +R   G   CGGTL++   VLTA HC+      K ET    +V I LG +   + SG E+ F+V + + HP+Y   + Y   ND+ L+K D   + + +        Q     E G+RC + GWG +   G  +   LQ+A++ +V    C   +    +  S LCAG       AC+GDSGGP+ C+N     IQ G  S+G   C  P    V+ +V  V +WI
Sbjct:    1 MLIRITSGSCGVSHHARIVGGTTAQQGGWPWQAQLRTSTGYPYCGGTLIHPEWVLTATHCL------KGETAS--RVHIRLGAHRRTKGSGNEQDFRVIRLVTHPNYHRPVGY--ANDIALLKLDRPAKLDRYVNFACLPDQVPEPKE-GDRCYITGWGKLSSHGS-APDILQQATVPVVGRARCMKAYRGQIH-ESMLCAGLDAGGVDACQGDSGGPMVCENAGRFYIQ-GATSWG-NGCAAPGKFGVYARVKYVLDWI 245          
BLAST of Trypsin-like serine protease vs. Ensembl Nematostella
Match: EDO44207 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RW60])

HSP 1 Score: 111.694 bits (278), Expect = 1.201e-29
Identity = 75/247 (30.36%), Postives = 125/247 (50.61%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGE-HICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTA-ACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWITR 797
            ++ING  +  H +P  +S+R     H+CGG L+++  V+TA+HCI        + L      +V+G + +   +  EK  +V++ L HP Y    S  + ND+ LI+    V+ +    +      N      G++C + GWG+++   + S   LQ+A L I S   C   + ++    ++LCAG      A  C GDSGGPL C++  +  +  G VSYG++ C   +  TVF +V+   +WI R
Sbjct:    2 RIINGQNAQPHSWPWQISLRQGRRFHLCGGALISDRWVVTASHCI-------HDDLNPGSYMVVVGAHRMSGSTSVEKTIRVSQVLEHPRYD---SQRDVNDIALIELATPVEFDSAGKVGTVCLTNQRPAP-GKQCYITGWGAVNGHTQQSPDILQQAMLPIASHENCRRKYGDVSST-AHLCAGEARSDAAGGCNGDSGGPLVCEDNGSWFLH-GAVSYGMKYCPTTHY-TVFARVASYTDWIKR 234          
BLAST of Trypsin-like serine protease vs. Ensembl Nematostella
Match: EDO38278 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDB3])

HSP 1 Score: 111.309 bits (277), Expect = 1.659e-29
Identity = 77/248 (31.05%), Postives = 124/248 (50.00%), Query Frame = 3
Query:   66 VINGTQSILHEYPQMVSIRLLGE---HICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYE-MSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYEC--AYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            ++ G  +   E+P  VS++L      HICGG +++   VLTAAHC+        +  +   +++ +GE+ L  V G E++  V + + H +Y+Y  + Y    D  L+K    +  N  QY++     + S +  G  C V GWGS +  G  S  +LQ+  L +V+  +C   Y+       P   CAG+  ++ A C GDSGGPL C+ G  +   +G+ S+G   C Q   P VF+ V    +WI
Sbjct:    4 IMGGANAEHGEWPWQVSMKLNSSSLPHICGGNVISPWWVLTAAHCV--------QDERASNIKLTMGEWRLFNVDGTEQVIPVERIISHANYSYNTVDY----DYALLKLTRPL--NFTQYVQPVCLPD-SDFPAGTLCYVTGWGSTNYRGSPSPNYLQEVGLPLVNHSQCHATYLTASRKITPRMRCAGTEGVAKAVCSGDSGGPLVCERG-GRWFLMGLSSWGWV-CPQAR-PKVFSDVLAAMDWI 233          
BLAST of Trypsin-like serine protease vs. Ensembl Medaka
Match: GZMM (duodenase-1 [Source:NCBI gene;Acc:101164468])

HSP 1 Score: 125.561 bits (314), Expect = 1.766e-34
Identity = 78/262 (29.77%), Postives = 123/262 (46.95%), Query Frame = 3
Query:    9 LNILIVFLIFISTVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQ-NNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            + ++ V L F+   +    ++ G  +  H  P M S+++ G+HICGG L+ E+ VLTAAHC  P+              +VLG   L+      + F+V +++ HP+Y  E+S    ND+ L+K DG+ +   E Q I         T     +C   GWG I    R  +  LQ+  + I++   C   +  +  + S +C          C GDSGGP+ C          GIVS+    CG P+ P V+T++S   +WI
Sbjct:    4 MAVMFVLLFFMGGAAGS-HIVGGRDAAPHSRPYMASLQVRGQHICGGALVREDFVLTAAHCDLPI-----------PFTVVLGADLLQENEPSRQTFRVLRSIPHPNYN-ELS----NDIMLLKLDGRARLTPEVQVIPLKS----GTVGPASQCITAGWGDIG-DNRTIANRLQEVDVTILAQRTCRQRWGNVRVLRSMVCGVGARSFQGFCSGDSGGPMICDGE-----AAGIVSFSGRRCGDPSTPDVYTRISSFRSWI 238          
BLAST of Trypsin-like serine protease vs. Ensembl Medaka
Match: ENSORLT00000025367.2 (granzyme E [Source:NCBI gene;Acc:101158094])

HSP 1 Score: 112.079 bits (279), Expect = 2.646e-29
Identity = 78/269 (29.00%), Postives = 127/269 (47.21%), Query Frame = 3
Query:   12 NILIVFLIFISTVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHC---IWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNIS-TYEIGERCEVVGWG--SIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEP-CGQPNVPTVFTKVSEVANWITR 797
            + L V L+  +  ++E  +  G  +  H  P M S++  G+H CGG L+ ++ VLT+AHC   + PMT             +VLG +D+ +    ++ F+V K   HPDY  E +    ND+ LIK + K + N  +Y+K  E    +       +C V GWG  ++D   + +S  L++A+       EC  I+ E       +C      +   C+GDSGGPL C         +G+ ++  +  C  P  P VF K++    WI +
Sbjct:    6 SFLCVLLMVSTAGASEGGIFGGRVAKPHSRPYMASLQFQGQHSCGGILIRDDYVLTSAHCKNDVQPMT-------------VVLGAHDISKKEKTQQHFKVQKYNQHPDYKCEQA----NDIMLIKLETKAKLN--KYVKPIELSKKNGDIRANIKCAVAGWGKTAVD---KPASNVLKEATEETQFKAECNNIWKEHFNSTQMICTKFSRKTGGICQGDSGGPLICNTK-----PLGLAAFTKQDNCNDPKFPHVFIKINFYLPWIKK 247          
BLAST of Trypsin-like serine protease vs. Ensembl Medaka
Match: si:dkey-32n7.7 (si:dkey-32n7.7 [Source:ZFIN;Acc:ZDB-GENE-121214-179])

HSP 1 Score: 113.235 bits (282), Expect = 3.009e-29
Identity = 74/245 (30.20%), Postives = 126/245 (51.43%), Query Frame = 3
Query:   63 KVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIK-YTEYQNISTYEIGERCEVVGWGSIDPSGRGS-SRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            K++ G  ++   +P   S++  G+H CGG+L+N+  VLTAAHC+              K+ + LG  +LE  +  E   +V   +VHPD  ++   +N ND+ L++    V  +   YI+      + S +  G    V GWG I         + +Q+ ++ ++ S +C  ++  +    + +CAG       +C+GDSGGP+  K G +  IQ GIVS+G+  C +PN+P V+++VS    WI
Sbjct:   39 KIVGGADAVPGSWPWQASLQYFGKHFCGGSLINKEWVLTAAHCV--------AGTSTKKLLVSLGRQNLEGKNPNEVSRRVAAIIVHPD--FDRGTMN-NDIALVRLSSPVPFS--HYIRPVCLAASASVFNNGTGSWVTGWGHIKEGELLPFPQTIQEVAVPVIGSRQCNCLYGVINITSNMICAGRLDGGKDSCQGDSGGPMLTKLG-SVWIQSGIVSFGIG-CARPNLPGVYSRVSRYQTWI 268          
BLAST of Trypsin-like serine protease vs. Ensembl Medaka
Match: zmp:0000001088 (trypsin-3-like [Source:NCBI gene;Acc:101160980])

HSP 1 Score: 109.383 bits (272), Expect = 3.862e-28
Identity = 86/250 (34.40%), Postives = 121/250 (48.40%), Query Frame = 3
Query:   48 VSAEWKVINGTQSILHEYPQMVSIRLLG-EHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIP-SNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            V A+ +++ G   + H    +VSI+    +H CGG L+N+  V+TAAHC           +   ++ +V G+Y L    G E+  Q    L HPDY    S  N +D+ LIK    V  N +  +     Q+ +  E G+ C V GWG   PSG      L+  +L IVS  +C    +    I  S LCAG       AC+GDSGGPL C NG       G+VS+G + C +   P V+T VS+   WI
Sbjct:   21 VLAQERIVGGYVPVPHSIKYIVSIQTTDRQHFCGGFLINKFWVITAAHC----------NIGLNRMVMVAGDYSLTVYEGTEQEIQPQILLPHPDYN---SVTNNSDIMLIKLRAPVFLNSYISVVLLPRQDATIAE-GKMCRVSGWGYTAPSGGQIPSTLRTVTLPIVSMEKCNSSDSFNGSITESMLCAGYSLGGKDACQGDSGGPLVC-NGR----AYGVVSWG-KGCAEAQFPGVYTAVSKFRKWI 250          
BLAST of Trypsin-like serine protease vs. Ensembl Medaka
Match: ENSORLT00000020234.2 (trypsin-like [Source:NCBI gene;Acc:101162676])

HSP 1 Score: 108.997 bits (271), Expect = 5.267e-28
Identity = 80/262 (30.53%), Postives = 122/262 (46.56%), Query Frame = 3
Query:   12 NILIVFLIFISTVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWITR 797
            + L++ L  I T     ++I GT    H      S+     H CGGTL++   V +AAHC      W+      Y +++VLGE++L +V G E+IF V+  + H  Y Y   ++  ND+ L+K D     ++         +N    E   +C V GWG      +  S  L   S++  S+    Y +       + +CAGS      +C+GDSGGPL C   +      GIVS+G+  CG  + P V+TKV     WI +
Sbjct:    9 HFLLILLSVILTGIHGGRIIGGTVVQPHSLKYQASLLYRDSHFCGGTLIHPQWVASAAHC------WR----PNYMIKVVLGEHNLNKVEGSEQIFSVSLIIKHYQYNY---WILDNDIMLLKLDRPANISDMVEPAPLPEKNAPPVENFSQCTVSGWGVTGLYSQSLSSELMSVSVDYFSNCWSFYYYYSFMITSNMICAGSLDGGKDSCQGDSGGPLVCNGKFE-----GIVSWGIG-CGYASYPGVYTKVRNYLIWIDQ 251          
BLAST of Trypsin-like serine protease vs. Planmine SMEST
Match: SMESG000042396.1 (SMESG000042396.1)

HSP 1 Score: 368.622 bits (945), Expect = 1.086e-130
Identity = 178/179 (99.44%), Postives = 179/179 (100.00%), Query Frame = 3
Query:   12 NILIVFLIFISTVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNI 548
            NILIVFLIFISTVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLN+
Sbjct:    5 NILIVFLIFISTVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNV 183          
BLAST of Trypsin-like serine protease vs. Planmine SMEST
Match: SMESG000042388.1 (SMESG000042388.1)

HSP 1 Score: 322.783 bits (826), Expect = 7.806e-112
Identity = 150/236 (63.56%), Postives = 184/236 (77.97%), Query Frame = 3
Query:   90 LHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWITR 797
            +HEYPQMVSI++ G H CGGTLLN NTVLTAAHC+W M +W L T K Y ++I LGEYDL ++ G EKIFQVTK ++HP YT + +Y+ GNDV LIKFDG V N ++ YIKY E    S  + G+RCEVVGWGS+ P G+ +SRFLQK ++NI+S+ EC+ IFT + Y+ SN+CAGSG LST+ACKGDSGGPLFC +G   +IQVGIVSYG  PCGQ NVPTVFT+VSEVA WI +
Sbjct:    1 MHEYPQMVSIKVQGSHFCGGTLLNGNTVLTAAHCVWSMRNWNLNTFKSYSMKIYLGEYDLYQMYGVEKIFQVTKVIIHPSYTSDFAYVYGNDVALIKFDGYVSNTQYNYIKYGELDLNSITKPGDRCEVVGWGSMLPPGQDTSRFLQKTAINILSNSECSRIFTNVRYVASNVCAGSGSLSTSACKGDSGGPLFCTDGRRHHIQVGIVSYGRVPCGQANVPTVFTRVSEVARWINQ 236          
BLAST of Trypsin-like serine protease vs. Planmine SMEST
Match: SMESG000042349.1 (SMESG000042349.1)

HSP 1 Score: 322.013 bits (824), Expect = 1.703e-111
Identity = 150/236 (63.56%), Postives = 185/236 (78.39%), Query Frame = 3
Query:   90 LHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWITR 797
            +HEYPQMVSI++ G H CGGTLLN NTVLTAAHC+W M +W L T K Y ++I LGEYDL ++ G EKIFQVTK ++HP YT + +Y+ GNDV LIKFDG V N ++ YIKY E    S  + G+RCEVVGWGS+ P G+ +SRFLQKA++NI+S+ EC+ IFT + Y+ SN+CAGSG LST+AC+GDSGGPLFC +G   +IQVGIVSYG  PCGQ NVPTVFT+VSEVA WI +
Sbjct:    1 MHEYPQMVSIKVQGSHSCGGTLLNGNTVLTAAHCVWSMRNWNLNTFKSYSMKIYLGEYDLYQMYGVEKIFQVTKVIIHPSYTSDFAYVYGNDVALIKFDGYVSNTQYNYIKYGELDLNSITKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINILSTSECSRIFTNVWYVASNVCAGSGSLSTSACRGDSGGPLFCTDGRRHHIQVGIVSYGQVPCGQANVPTVFTRVSEVARWINQ 236          
BLAST of Trypsin-like serine protease vs. Planmine SMEST
Match: SMESG000042386.1 (SMESG000042386.1)

HSP 1 Score: 300.442 bits (768), Expect = 1.709e-102
Identity = 141/261 (54.02%), Postives = 185/261 (70.88%), Query Frame = 3
Query:   21 IVFLIFI----STVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWI 791
            +VF +F+      +S   K ING+Q I+H++PQMVSI+L+  HICGGTLLN NTVLT AHCIW +  W  +  K YK  I LGEYDL  + G EK FQVTK ++HP YT EMS+  GNDV L+KFDG ++N ++ YIKY         + G+RCEVVGWGS+ P G  ++R LQ+ASL ++S+ +C+  F  + YI SN+CAG+G+   +ACKGD GGPLFC +G N+++QVGIVSYG  PCGQP +P VFT++S VA WI
Sbjct:    5 VVFHLFVVLSFCAISTALKTINGSQEIMHQFPQMVSIKLMDNHICGGTLLNGNTVLTTAHCIWDLRYWNNDYFKYYKFRIFLGEYDLHYMYGIEKDFQVTKIIMHPSYTSEMSFYYGNDVALVKFDGYIENTDYDYIKYNVLDINGISKPGDRCEVVGWGSLLPPGENTARLLQEASLIVMSNEDCSKTFYNVKYIDSNICAGTGNSDASACKGDLGGPLFCTDGKNQHVQVGIVSYGHVPCGQPYIPIVFTRISTVAKWI 265          
BLAST of Trypsin-like serine protease vs. Planmine SMEST
Match: SMESG000042389.1 (SMESG000042389.1)

HSP 1 Score: 295.819 bits (756), Expect = 2.888e-101
Identity = 134/235 (57.02%), Postives = 179/235 (76.17%), Query Frame = 3
Query:   90 LHEYPQMVSIRLLGEHICGGTLLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQVTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTYEIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPSNLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVPTVFTKVSEVANWIT 794
            +H+YPQ+VSI+L G+HICGGTLLN NTVLTAAHC+W M  + ++  + +KV++VLGEYDL  +SG+EK+ QV K ++HP Y    +Y++GNDV L+KFDG + N+E +Y+KY +         G+RCEVVGWGS+ P G  +S+FLQKA L+IVS+ EC+  +  +  +PSN+CAGSG LS +ACKGDSGGPLFC++  N+ IQVGIVSY   PCGQ N PTV+T++SEVA WIT
Sbjct:    1 MHQYPQIVSIQLDGDHICGGTLLNGNTVLTAAHCVWDMRYYTMDWFEYHKVKVVLGEYDLHHISGKEKVLQVNKIMMHPTYKRAKAYIDGNDVALMKFDGFISNDETEYVKYIKLDTDLKVSPGKRCEVVGWGSMQPDGEDTSQFLQKAFLDIVSTEECSKTYPNVQNVPSNICAGSGSLSISACKGDSGGPLFCRDPANRLIQVGIVSYSKMPCGQANKPTVYTRISEVAKWIT 235          
The following BLAST results are available for this feature:
BLAST of Trypsin-like serine protease vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
KLK133.994e-3234.00kallikrein related peptidase 13 [Source:HGNC Symbo... [more]
PRSS381.696e-3031.87serine protease 38 [Source:HGNC Symbol;Acc:HGNC:29... [more]
GZMK4.639e-3029.17granzyme K [Source:HGNC Symbol;Acc:HGNC:4711][more]
CTRL2.536e-2930.89chymotrypsin like [Source:HGNC Symbol;Acc:HGNC:252... [more]
KLK35.910e-2930.00kallikrein related peptidase 3 [Source:HGNC Symbol... [more]
back to top
BLAST of Trypsin-like serine protease vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
try-13.327e-2329.08TRYpsin-like protease [Source:UniProtKB/TrEMBL;Ac... [more]
svh-13.881e-2126.95HGF/MSP/plasminogen-like protein [Source:UniProtK... [more]
try-31.182e-1125.11TRYpsin-like protease [Source:UniProtKB/TrEMBL;Ac... [more]
try-31.597e-1125.11TRYpsin-like protease [Source:UniProtKB/TrEMBL;Ac... [more]
try-102.172e-722.35TRYpsin-like protease [Source:UniProtKB/TrEMBL;Ac... [more]
back to top
BLAST of Trypsin-like serine protease vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG328082.108e-3134.01gene:FBgn0052808 transcript:FBtr0070295[more]
CG119116.781e-2832.13gene:FBgn0031249 transcript:FBtr0078098[more]
l(2)k059116.869e-2732.13gene:FBgn0284244 transcript:FBtr0305556[more]
Sb2.821e-2631.40gene:FBgn0003319 transcript:FBtr0344008[more]
Sb2.821e-2631.40gene:FBgn0003319 transcript:FBtr0083250[more]
back to top
BLAST of Trypsin-like serine protease vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:ch73-182e20.47.082e-3132.57si:ch73-182e20.4 [Source:ZFIN;Acc:ZDB-GENE-131127-... [more]
si:dkeyp-93a5.21.599e-2933.07si:dkeyp-93a5.2 [Source:ZFIN;Acc:ZDB-GENE-131127-1... [more]
zgc:1232959.396e-2933.20zgc:123295 [Source:ZFIN;Acc:ZDB-GENE-051127-11][more]
zgc:1122851.993e-2831.50zgc:112285 [Source:ZFIN;Acc:ZDB-GENE-050913-132][more]
si:dkey-21e2.132.246e-2832.94si:dkey-21e2.13 [Source:ZFIN;Acc:ZDB-GENE-050208-3... [more]
back to top
BLAST of Trypsin-like serine protease vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
trim364.865e-3332.92tripartite motif containing 36 [Source:Xenbase;Acc... [more]
trim367.922e-3332.92tripartite motif containing 36 [Source:Xenbase;Acc... [more]
ENSXETT00000015569.17.479e-3131.40pep primary_assembly:Xenopus_tropicalis_v9.1:9:281... [more]
pcdh8l1.116e-3033.60protocadherin-8-like [Source:Xenbase;Acc:XB-GENE-1... [more]
ENSXETT00000030871.11.319e-3031.40pep primary_assembly:Xenopus_tropicalis_v9.1:9:282... [more]
back to top
BLAST of Trypsin-like serine protease vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Klk95.025e-3230.27kallikrein related-peptidase 9 [Source:MGI Symbol;... [more]
Klk135.963e-3230.29kallikrein related-peptidase 13 [Source:MGI Symbol... [more]
Gzmm5.613e-3032.13granzyme M (lymphocyte met-ase 1) [Source:MGI Symb... [more]
Klk81.562e-2928.41kallikrein related-peptidase 8 [Source:MGI Symbol;... [more]
Prss382.262e-2931.97protease, serine 38 [Source:MGI Symbol;Acc:MGI:268... [more]
back to top
BLAST of Trypsin-like serine protease vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9UKR3|KLK13_HUMAN1.918e-3134.00Kallikrein-13 OS=Homo sapiens OX=9606 GN=KLK13 PE=... [more]
sp|Q6DT45|KLK3_MACFA2.629e-3030.11Prostate-specific antigen OS=Macaca fascicularis O... [more]
sp|P09582|ESTA_CANLF3.561e-3030.56Arginine esterase OS=Canis lupus familiaris OX=961... [more]
sp|P33619|KLK3_MACMU6.161e-3029.75Prostate-specific antigen OS=Macaca mulatta OX=954... [more]
sp|A1L453|PRS38_HUMAN8.146e-3031.87Serine protease 38 OS=Homo sapiens OX=9606 GN=PRSS... [more]
back to top
BLAST of Trypsin-like serine protease vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H2DRI73.299e-8158.38Trypsin-like serine protease OS=Dugesia japonica O... [more]
A0A1I8GT164.477e-4633.58Peptidase S1 domain-containing protein OS=Macrosto... [more]
A0A1I8JAW51.117e-4533.58Peptidase S1 domain-containing protein OS=Macrosto... [more]
B0X1E92.953e-3636.89Chymotrypsin A OS=Culex quinquefasciatus OX=7176 G... [more]
A0A1I8JHD89.661e-3630.83Peptidase S1 domain-containing protein OS=Macrosto... [more]
back to top
BLAST of Trypsin-like serine protease vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000043830.18.383e-3135.14elastase-1-like [Source:NCBI gene;Acc:103036513][more]
ENSAMXT00000048530.11.154e-3031.08pep primary_assembly:Astyanax_mexicanus-2.0:13:202... [more]
ENSAMXT00000001125.23.815e-3033.06mast cell protease 4-like [Source:NCBI gene;Acc:10... [more]
si:dkey-78l4.132.980e-2930.80si:dkey-78l4.13 [Source:ZFIN;Acc:ZDB-GENE-060503-4... [more]
ENSAMXT00000039451.15.471e-2932.67trypsin-like [Source:NCBI gene;Acc:111192060][more]
back to top
BLAST of Trypsin-like serine protease vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000008289.17.392e-3332.92pep scaffold:Pmarinus_7.0:GL480450:20964:30734:1 g... [more]
ENSPMAT00000002946.15.222e-2628.63pep scaffold:Pmarinus_7.0:GL477988:107792:112981:-... [more]
ENSPMAT00000005493.11.077e-2528.63pep scaffold:Pmarinus_7.0:GL476632:243920:250733:-... [more]
si:dkey-33m11.81.347e-2532.65si:dkey-33m11.8 [Source:ZFIN;Acc:ZDB-GENE-141215-4... [more]
ENSPMAT00000006511.11.953e-2528.90pep scaffold:Pmarinus_7.0:GL476457:197255:208031:1... [more]
back to top
BLAST of Trypsin-like serine protease vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Trypsin-like serine protease vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO309081.760e-3232.10Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO399109.653e-3231.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO424201.048e-2931.92Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO442071.201e-2930.36Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO382781.659e-2931.05Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Trypsin-like serine protease vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GZMM1.766e-3429.77duodenase-1 [Source:NCBI gene;Acc:101164468][more]
ENSORLT00000025367.22.646e-2929.00granzyme E [Source:NCBI gene;Acc:101158094][more]
si:dkey-32n7.73.009e-2930.20si:dkey-32n7.7 [Source:ZFIN;Acc:ZDB-GENE-121214-17... [more]
zmp:00000010883.862e-2834.40trypsin-3-like [Source:NCBI gene;Acc:101160980][more]
ENSORLT00000020234.25.267e-2830.53trypsin-like [Source:NCBI gene;Acc:101162676][more]
back to top
BLAST of Trypsin-like serine protease vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000042396.11.086e-13099.44SMESG000042396.1[more]
SMESG000042388.17.806e-11263.56SMESG000042388.1[more]
SMESG000042349.11.703e-11163.56SMESG000042349.1[more]
SMESG000042386.11.709e-10254.02SMESG000042386.1[more]
SMESG000042389.12.888e-10157.02SMESG000042389.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013511 ID=SMED30013511|Name=Trypsin-like serine protease|organism=Schmidtea mediterranea sexual|type=transcript|length=841bp
CTTCCGATCTCAATATTTTAATAGTTTTCTTGATTTTTATTTCTACAGTT
TCAGCAGAATGGAAGGTTATCAATGGAACACAGTCCATATTGCACGAATA
CCCTCAAATGGTTTCAATACGTTTACTGGGAGAGCACATTTGTGGAGGAA
CATTGCTTAATGAGAATACTGTTCTGACGGCTGCTCACTGCATCTGGCCA
ATGACTGACTGGAAATTGGAAACCTTAAAAGAATATAAAGTCGAGATTGT
GCTTGGTGAATATGATTTAGAGAGGGTTTCAGGAGAGGAAAAGATTTTTC
AAGTAACAAAAAATCTGGTACATCCAGATTATACATATGAAATGTCTTAT
TTGAATGGGAATGATGTCGGTCTAATAAAATTCGATGGAAAAGTTCAAAA
TAATGAATTTCAATACATTAAATACACCGAATATCAAAATATTTCAACTT
ATGAAATAGGAGAGCGCTGTGAAGTTGTGGGATGGGGTAGCATAGACCCG
TCCGGAAGAGGCAGTTCTAGATTCCTGCAAAAAGCATCTTTAAATATTGT
TTCGAGCTACGAATGTGCTTATATATTTACTGAACTACCATATATTCCCA
GTAATCTTTGTGCTGGTTCTGGGCACTTATCCACTGCTGCTTGTAAAGGC
GATTCTGGAGGACCACTGTTTTGTAAAAATGGTTATAATAAAAATATTCA
AGTTGGAATAGTGAGCTATGGCTTAGAACCTTGCGGACAACCAAATGTAC
CGACGGTTTTTACAAAGGTGTCTGAAGTGGCAAATTGGATAACCCGTAAA
GCTAAATCTAGCAAAAAACAATTAAAAAAATTTACTCGGAA
back to top

protein sequence of SMED30013511-orf-1

>SMED30013511-orf-1 ID=SMED30013511-orf-1|Name=SMED30013511-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=279bp
SDLNILIVFLIFISTVSAEWKVINGTQSILHEYPQMVSIRLLGEHICGGT
LLNENTVLTAAHCIWPMTDWKLETLKEYKVEIVLGEYDLERVSGEEKIFQ
VTKNLVHPDYTYEMSYLNGNDVGLIKFDGKVQNNEFQYIKYTEYQNISTY
EIGERCEVVGWGSIDPSGRGSSRFLQKASLNIVSSYECAYIFTELPYIPS
NLCAGSGHLSTAACKGDSGGPLFCKNGYNKNIQVGIVSYGLEPCGQPNVP
TVFTKVSEVANWITRKAKSSKKQLKKFTR
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
PLANA:0002075pigment cell
Vocabulary: INTERPRO
TermDefinition
IPR001314Peptidase_S1A
IPR001254Trypsin_dom
IPR033116TRYPSIN_SER
IPR018114TRYPSIN_HIS
IPR009003Peptidase_S1_PA
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
Vocabulary: molecular function
TermDefinition
GO:0004252serine-type endopeptidase activity
GO:0008233peptidase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001314Peptidase S1A, chymotrypsin familyPRINTSPR00722CHYMOTRYPSINcoord: 114..128
score: 26.8
coord: 208..220
score: 61.15
coord: 45..60
score: 60.14
IPR001254Serine proteases, trypsin domainSMARTSM00020trypsin_2coord: 18..260
e-value: 5.5E-64
score: 228.5
IPR001254Serine proteases, trypsin domainPFAMPF00089Trypsincoord: 20..260
e-value: 1.7E-48
score: 165.4
IPR001254Serine proteases, trypsin domainPROSITEPS50240TRYPSIN_DOMcoord: 19..265
score: 29.194
IPR001254Serine proteases, trypsin domainCDDcd00190Tryp_SPccoord: 19..260
e-value: 3.97612E-65
score: 201.351
NoneNo IPR availableGENE3DG3DSA:2.40.10.10coord: 11..266
e-value: 3.3E-58
score: 198.9
NoneNo IPR availablePANTHERPTHR24276FAMILY NOT NAMEDcoord: 18..267
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..15
score: 0.844
IPR018114Serine proteases, trypsin family, histidine active sitePROSITEPS00134TRYPSIN_HIScoord: 55..60
IPR033116Serine proteases, trypsin family, serine active sitePROSITEPS00135TRYPSIN_SERcoord: 209..220
IPR009003Peptidase S1, PA clanSUPERFAMILYSSF50494Trypsin-like serine proteasescoord: 7..265