Ribosome assembly factor mrt4
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30013497 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Homology
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Human
Match: MRTO4 (MRT4 homolog, ribosome maturation factor [Source:HGNC Symbol;Acc:HGNC:18477]) HSP 1 Score: 110.538 bits (275), Expect = 1.864e-28 Identity = 66/190 (34.74%), Postives = 106/190 (55.79%), Query Frame = 1 Query: 106 KKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEGNP----------LTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNS 645 K+N I+ ++ + + +F+ ++ N+R + +K IR + S++ FGK K+ AL +P +++ L G LLFT R E V+E TKYT +++AR G +V + PL+ F + P LR LG+P +K GV+ L+ DY++CK+G+ L +R+LK F + AEF V IK M S Sbjct: 23 KQNLIEELRKCVDTYKYLFIFSVANMRNSKLKDIRNAWKHSRMFFGKNKVMMVALGRSPSDEYKDNLHQVSKRLRGEVGLLFTNRTKEEVNEWFTKYTEMDYARAGNKAAFTVSLDPGPLEQFPHSMEPQLRQLGLPTALKRGVVTLLSDYEVCKEGDVLTPEQARVLKLFGYEMAEFKVTIKYMWDSQS 212
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Celegans
Match: F10E7.5 (Ribosome assembly factor mrt4 [Source:UniProtKB/TrEMBL;Acc:Q19302]) HSP 1 Score: 79.7221 bits (195), Expect = 1.395e-17 Identity = 47/183 (25.68%), Postives = 93/183 (50.82%), Query Frame = 1 Query: 112 NFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEG-----------NPLTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKA 627 N ++ V+ + Q+ +F+ I N+R+ IR+ + ++ F K G + + +L+G L+FT + + V E ++ + ++AR G + ++V + P+ F + P LR LG+P ++ GVI L Q +++CK+GE L +++LK F ++ ++F + KA Sbjct: 25 NLVNEVRASVDQYKNLFIFTIANMRSTRFIAIRQKYKENSRFFFGKNNVISIALGKQKSDEYANQLHKASAILKGQCGLMFTNMSKKEVEAEFSEASEEDYARVGDVATETVVLPEGPISQFAFSMEPQLRKLGLPTKLDKGVITLYQQFEVCKEGEPLTVEQAKILKHFEVKMSQFRLIFKA 207
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Celegans
Match: F10E7.5 (Ribosome assembly factor mrt4 [Source:UniProtKB/TrEMBL;Acc:Q19302]) HSP 1 Score: 79.7221 bits (195), Expect = 1.395e-17 Identity = 47/183 (25.68%), Postives = 93/183 (50.82%), Query Frame = 1 Query: 112 NFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEG-----------NPLTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKA 627 N ++ V+ + Q+ +F+ I N+R+ IR+ + ++ F K G + + +L+G L+FT + + V E ++ + ++AR G + ++V + P+ F + P LR LG+P ++ GVI L Q +++CK+GE L +++LK F ++ ++F + KA Sbjct: 25 NLVNEVRASVDQYKNLFIFTIANMRSTRFIAIRQKYKENSRFFFGKNNVISIALGKQKSDEYANQLHKASAILKGQCGLMFTNMSKKEVEAEFSEASEEDYARVGDVATETVVLPEGPISQFAFSMEPQLRKLGLPTKLDKGVITLYQQFEVCKEGEPLTVEQAKILKHFEVKMSQFRLIFKA 207
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Fly
Match: RpLP0-like (gene:FBgn0033485 transcript:FBtr0088425) HSP 1 Score: 100.523 bits (249), Expect = 7.122e-25 Identity = 54/192 (28.12%), Postives = 102/192 (53.12%), Query Frame = 1 Query: 82 VERKTKPHKKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEG-----------NPLTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIK 624 +RK K+ +D ++ +G++ IFV + N+R +L+K +R++ + K + G + L++ L G LLFT+++ E V E Y ++E+AR+G + ++V + A PL+ F + P LR LG+P +++ G++ L DY +C++G+ L +R+LK A+F + +K Sbjct: 15 TDRKGLAWKQRIVDDIRFCVGKYPNIFVFQVQNMRNSLLKDLRQELKKNSRFIFGKNRVMQIGLGRTKSEEVEPELHKLSKRLTGQVGLLFTDKSKEEVLEWAENYWAVEYARSGFVATETVTLPAGPLEDFAHSMEPHLRSLGLPTKLEKGIVTLYSDYTVCEEGKVLTPEQARILKLVGKPMAKFRLTMK 206
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Zebrafish
Match: mrto4 (MRT4 homolog, ribosome maturation factor [Source:ZFIN;Acc:ZDB-GENE-050417-142]) HSP 1 Score: 98.9821 bits (245), Expect = 2.540e-24 Identity = 60/190 (31.58%), Postives = 98/190 (51.58%), Query Frame = 1 Query: 106 KKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEGNP----------LTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNS 645 K+N I+ ++ + +FV ++ N+R N +K IR + S+ FGK K+ AL P L+ L G +LFT + + V E + +++AR G +V + PL F + P LR LG+P +K GV+ L++D+++CK+G+ L +R+LK F + AEF + IK M S Sbjct: 23 KQNLIEELRKCADIYRYVFVFSVENMRNNKLKDIRTAWKHSRFFFGKNKVMMIALGKGPTDEYKDNLHKLSRFLRGEVGVLFTNKTKDEVQEYFGNFKEVDYARAGNTASMAVTLDEGPLDQFPHSMEPQLRQLGLPTALKKGVVTLIKDFEVCKEGDTLTPEQARILKLFGFEMAEFKLSIKCMWNSES 212
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Xenopus
Match: mrto4 (MRT4 homolog, ribosome maturation factor [Source:NCBI gene;Acc:448696]) HSP 1 Score: 90.5077 bits (223), Expect = 7.540e-22 Identity = 46/117 (39.32%), Postives = 70/117 (59.83%), Query Frame = 1 Query: 283 LTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAML 633 L++ L+G LLFT R + V E ++ ++FAR G +V + A PL+ F + P LR LG+P +K GVI L+ DY++CK+G+ L +R+LK F+ AEF V IK++ Sbjct: 1 LSKCLKGEVGLLFTNRTEKEVKEWFDQFKDMDFARAGNKATYAVVLDAGPLEQFTHSMEPQLRQLGLPTALKKGVITLLSDYEVCKEGDVLTPEQARILKLFSFM-AEFKVSIKSIW 116
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Mouse
Match: Mrto4 (mRNA turnover 4, ribosome maturation factor [Source:MGI Symbol;Acc:MGI:1917152]) HSP 1 Score: 112.464 bits (280), Expect = 2.171e-29 Identity = 68/190 (35.79%), Postives = 106/190 (55.79%), Query Frame = 1 Query: 106 KKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEGNP----------LTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNS 645 K+N I+ ++ + + +F+ ++ N+R + +K IR + S++ FGK K+ AL +P +++ L G LLFT R E V+E TKYT ++FAR G +V + PLK F + P LR LG+P +K GV+ L+ DY++CK+G+ L +R+LK F + AEF V IK M S Sbjct: 23 KQNLIEELRKCVDTYKYLFIFSVANMRNSKLKDIRNAWKHSRMFFGKNKVMMVALGRSPSDEYKDNLHQVSKKLRGEVGLLFTNRTKEEVNEWFTKYTEMDFARAGNKATLTVSLDPGPLKQFPHSMEPQLRQLGLPTALKKGVVTLLSDYEVCKEGDVLTPEQARILKLFGYEMAEFKVIIKYMWDAQS 212
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Mouse
Match: Mrto4 (mRNA turnover 4, ribosome maturation factor [Source:MGI Symbol;Acc:MGI:1917152]) HSP 1 Score: 106.301 bits (264), Expect = 5.309e-27 Identity = 67/190 (35.26%), Postives = 105/190 (55.26%), Query Frame = 1 Query: 106 KKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEGNP----------LTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNS 645 K+N I+ ++ + + +F+ ++ N+R + +K IR + S++ FGK K+ AL +P +++ L G LLFT R E V+E TKYT ++FAR G +V + PLK F + P LR LG+P +K GV+ L+ DY++CK+G+ L +R+L F + AEF V IK M S Sbjct: 23 KQNLIEELRKCVDTYKYLFIFSVANMRNSKLKDIRNAWKHSRMFFGKNKVMMVALGRSPSDEYKDNLHQVSKKLRGEVGLLFTNRTKEEVNEWFTKYTEMDFARAGNKATLTVSLDPGPLKQFPHSMEPQLRQLGLPTALKKGVVTLLSDYEVCKEGDVLTPEQARIL-LFGYEMAEFKVIIKYMWDAQS 211
BLAST of Ribosome assembly factor mrt4 vs. UniProt/SwissProt
Match: sp|Q9D0I8|MRT4_MOUSE (mRNA turnover protein 4 homolog OS=Mus musculus OX=10090 GN=Mrto4 PE=1 SV=1) HSP 1 Score: 112.464 bits (280), Expect = 1.519e-28 Identity = 68/190 (35.79%), Postives = 106/190 (55.79%), Query Frame = 1 Query: 106 KKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEGNP----------LTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNS 645 K+N I+ ++ + + +F+ ++ N+R + +K IR + S++ FGK K+ AL +P +++ L G LLFT R E V+E TKYT ++FAR G +V + PLK F + P LR LG+P +K GV+ L+ DY++CK+G+ L +R+LK F + AEF V IK M S Sbjct: 23 KQNLIEELRKCVDTYKYLFIFSVANMRNSKLKDIRNAWKHSRMFFGKNKVMMVALGRSPSDEYKDNLHQVSKKLRGEVGLLFTNRTKEEVNEWFTKYTEMDFARAGNKATLTVSLDPGPLKQFPHSMEPQLRQLGLPTALKKGVVTLLSDYEVCKEGDVLTPEQARILKLFGYEMAEFKVIIKYMWDAQS 212
BLAST of Ribosome assembly factor mrt4 vs. UniProt/SwissProt
Match: sp|Q9UKD2|MRT4_HUMAN (mRNA turnover protein 4 homolog OS=Homo sapiens OX=9606 GN=MRTO4 PE=1 SV=2) HSP 1 Score: 110.538 bits (275), Expect = 8.953e-28 Identity = 66/190 (34.74%), Postives = 106/190 (55.79%), Query Frame = 1 Query: 106 KKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEGNP----------LTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNS 645 K+N I+ ++ + + +F+ ++ N+R + +K IR + S++ FGK K+ AL +P +++ L G LLFT R E V+E TKYT +++AR G +V + PL+ F + P LR LG+P +K GV+ L+ DY++CK+G+ L +R+LK F + AEF V IK M S Sbjct: 23 KQNLIEELRKCVDTYKYLFIFSVANMRNSKLKDIRNAWKHSRMFFGKNKVMMVALGRSPSDEYKDNLHQVSKRLRGEVGLLFTNRTKEEVNEWFTKYTEMDYARAGNKAAFTVSLDPGPLEQFPHSMEPQLRQLGLPTALKRGVVTLLSDYEVCKEGDVLTPEQARVLKLFGYEMAEFKVTIKYMWDSQS 212
BLAST of Ribosome assembly factor mrt4 vs. UniProt/SwissProt
Match: sp|A4FV84|MRT4_BOVIN (mRNA turnover protein 4 homolog OS=Bos taurus OX=9913 GN=MRTO4 PE=2 SV=1) HSP 1 Score: 110.153 bits (274), Expect = 1.420e-27 Identity = 66/190 (34.74%), Postives = 105/190 (55.26%), Query Frame = 1 Query: 106 KKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEGNP----------LTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNS 645 K+N I+ ++ + + +F+ ++ N+R + +K IR + S++ FGK K+ AL +P +++ L G LLFT R E V E TKYT +++AR G +V + PL+ F + P LR LG+P +K GV+ L+ DY++CK+G+ L +R+LK F + AEF V IK M S Sbjct: 23 KQNLIEELRKCVDTYKYLFIFSVANMRNSKLKDIRNAWKHSRMFFGKNKVMMVALGRSPSDEYKDNLHQVSKKLRGEVGLLFTNRTKEEVDEWFTKYTEMDYARAGNKATFTVNLDPGPLEQFPHSMEPQLRQLGLPTALKKGVVTLLSDYEVCKEGDVLTPEQARVLKLFGYEMAEFKVSIKYMWDAQS 212
BLAST of Ribosome assembly factor mrt4 vs. UniProt/SwissProt
Match: sp|Q86HD3|MRT4_DICDI (Ribosome assembly factor mrt4 OS=Dictyostelium discoideum OX=44689 GN=mrt4 PE=3 SV=1) HSP 1 Score: 98.2117 bits (243), Expect = 3.151e-23 Identity = 58/198 (29.29%), Postives = 99/198 (50.00%), Query Frame = 1 Query: 106 KKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSX------XXXXXXXXXXXXXEGNP----LTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNSLTNCEINS 669 KK + T+K ++ Q+ I++ N+R N +K +R +++ SK + + E P LTE LEG L FT + V E T Y+ +F R+G + ++++ + P+ + LR LG+P +K+GVI + ++Y +C+ G + S+LLK FN + +EF IK ++ T CE S Sbjct: 23 KKKLVSTIKDIVDQYKFIYLFTFENMRNNKLKSVRTEWSTSKFLFGKNKVLSVGLGKSEEDELKPNLHKLTEHLEGECGLFFTNEPKDKVFEYFTNYSEKDFPRSGFVSEETITIKEGPIVGMTHSMETYLRGLGLPTTLKNGVIFVDREYTLCEAGVAVTPEQSQLLKLFNHEISEFKFHIKGFWNEDEFTLCEEES 220
BLAST of Ribosome assembly factor mrt4 vs. UniProt/SwissProt
Match: sp|Q9USZ6|MRT4_SCHPO (Ribosome assembly factor mrt4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mrt4 PE=3 SV=1) HSP 1 Score: 92.0485 bits (227), Expect = 7.849e-21 Identity = 63/201 (31.34%), Postives = 99/201 (49.25%), Query Frame = 1 Query: 85 ERKTKPHKKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEGNP----------LTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLK------------VFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEI 621 E+K K V+ L F +++ ++ N+R +KRIR+D+ S+I GK K+ KAL P LT++L G LLFT + V + +FAR G + + + A P+ + L P +R LGMP +K+GV+ L+ D+ +C +G+QL++ +RLLK F I AEF V + Sbjct: 16 EKKGHEGKAALFSGVQQSLDSFDYMWIFDVTNMRNTYLKRIRDDWKGSRIFMGKTKVMAKALGHTPEEEHAENVSKLTKLLHGAVGLLFTNSKPDEVIGYFESFVQNDFARAGAVAPFTHVIPAGPVYSRAGQIPVEDDILLTHTLEPQVRQLGMPTVLKNGVVTLLADFPLCTEGQQLDSRQTRLLKLFGITAAEFKVGL 216
BLAST of Ribosome assembly factor mrt4 vs. TrEMBL
Match: A0A419PIK2 (Ribosome assembly factor mrt4 (Fragment) OS=Clonorchis sinensis OX=79923 GN=Mrto4 PE=3 SV=1) HSP 1 Score: 120.168 bits (300), Expect = 9.704e-29 Identity = 72/241 (29.88%), Postives = 120/241 (49.79%), Query Frame = 1 Query: 91 KTKPHKKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFND----------SXXXXXXXXXXXXXXEGNPLTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAML--KDNSLTNCEINSGKSIENLPASVEVRCELLDDGKYYFMSHAIEEAAMD 777 K+ K+ I+ ++ + ++ +V+ NN R + +R D D + + K+ + L++ ++G LL T + + E+ S EFAR GV Q+V + A P F L P LR LG+PV++ GV+IL +DY CK+ ++L R+LK F +Q +EF V I A ++ L + +S + I +L +V V C+ LDDG+YYF+ A+ + D Sbjct: 7 KSASKKREHIEKIRRYVDSYNRAYVITFNNPRNQKISELRHDLKDGQIIFGKNKVTILALRKRLGKALRPHLDALSKFIKGQCALLLTNLSVRELREKFDALRSSEFARPGVPAPQTVTLAAGPCHKFTHTLEPYLRQLGLPVKLVRGVVILEEDYIACKRDQELTPEQCRVLKLFELQLSEFRVAIIASWSEEEGVLEVSDSDSRQMITSLAPAVRVTCQKLDDGQYYFIPEAVPDEPED 247
BLAST of Ribosome assembly factor mrt4 vs. TrEMBL
Match: A0A5K4FB50 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 117.087 bits (292), Expect = 1.524e-27 Identity = 72/237 (30.38%), Postives = 119/237 (50.21%), Query Frame = 1 Query: 91 KTKPHKKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFND----------SXXXXXXXXXXXXXXEGNPLTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNSLTN--CEINSGKSIENLPASVEVRCELLDDGKYYFMSHAIEE 765 K P KK I V+ + ++ ++V+ + N RT V IR+ + + GK + + L++ L+G LLF+ + + E+L + S E++R GV+ +Q+V + + PL F + P LR LGMPV++ GV+ L ++Y +C +G L+ R+LK F I+ +EF V + A+ D E +S +L V V C+ LDDG YYF+ E+ Sbjct: 17 KHAPKKKEHIAKVRQYVDEYRRVYVVTLQNPRTQKVSEIRKSMAKIKLLFGVNKVTTLALGKTHEDSYRPKLHLLSKYLKGQCALLFSRSSPAELREQLDAFRSSEYSRPGVIAEQTVWIASGPLPKFSHSMEPVLRQLGMPVKLVKGVVHLEREYLVCSRGNVLSPEQCRILKLFQIEISEFRVSLLAVWIDGEGVEELAEKDSCMMRTSLHPEVLVVCKQLDDGLYYFIPQPYEK 253
BLAST of Ribosome assembly factor mrt4 vs. TrEMBL
Match: C1LJE9 (Ribosome assembly factor mrt4 OS=Schistosoma japonicum OX=6182 PE=2 SV=1) HSP 1 Score: 116.701 bits (291), Expect = 2.271e-27 Identity = 73/238 (30.67%), Postives = 119/238 (50.00%), Query Frame = 1 Query: 88 RKTKPHKKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFND----------SXXXXXXXXXXXXXXEGNPLTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNSLTNCEINSGKSI---ENLPASVEVRCELLDDGKYYFMSHAIE 762 +K P KK + V+ L ++ ++V+ ++N RT V IR++ D + + GK + + L + L+G LLFT+ + + E+L + S E+ R G +Q+V++ + PL F + P LR LGMPV++ GV+ L +DY +C + L+ R+LK F I+ +EF V + A+ D E+ S +L V V C+ LDDG YYF+ E Sbjct: 16 QKHAPKKKQHVAKVRQYLDEYKRVYVVTLHNPRTQKVSEIRKNMPDIKLLFGINKVTVLALGKTHKDSYRPKLHHLCKYLKGQCALLFTKSSPSKLREQLDAFRSAEYCRPGAPAEQTVRIASGPLPKFPHTMEPVLRQLGMPVKLVRGVVHLERDYLVCNSEDVLSPEQCRILKLFQIEMSEFRVGLLAVWTDGDGVE-ELEEKDSCLMRTSLHPEVIVVCKQLDDGLYYFIPQPCE 252
BLAST of Ribosome assembly factor mrt4 vs. TrEMBL
Match: C1LJF0 (Ribosome assembly factor mrt4 OS=Schistosoma japonicum OX=6182 PE=2 SV=1) HSP 1 Score: 116.701 bits (291), Expect = 2.320e-27 Identity = 73/238 (30.67%), Postives = 119/238 (50.00%), Query Frame = 1 Query: 88 RKTKPHKKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFND----------SXXXXXXXXXXXXXXEGNPLTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNSLTNCEINSGKSI---ENLPASVEVRCELLDDGKYYFMSHAIE 762 +K P KK + V+ L ++ ++V+ ++N RT V IR++ D + + GK + + L + L+G LLFT+ + + E+L + S E+ R G +Q+V++ + PL F + P LR LGMPV++ GV+ L +DY +C + L+ R+LK F I+ +EF V + A+ D E+ S +L V V C+ LDDG YYF+ E Sbjct: 16 QKHAPKKKQHVAKVRQYLDEYKRVYVVTLHNPRTQKVSEIRKNMPDIKLLFGINKVTVLALGKTHKDSYRPKLHHLCKYLKGQCALLFTKSSPSELREQLDAFRSAEYCRPGAPAEQTVRIASGPLPKFPHTMEPVLRQLGMPVKLVRGVVHLERDYLVCNSEDVLSPEQCRILKLFQIEMSEFRVGLLAVWTDGDGVE-ELEEKDSCLMRTSLHPEVIVVCKQLDDGLYYFIPQPCE 252
BLAST of Ribosome assembly factor mrt4 vs. TrEMBL
Match: C1LJF1 (Ribosome assembly factor mrt4 OS=Schistosoma japonicum OX=6182 PE=2 SV=1) HSP 1 Score: 116.701 bits (291), Expect = 2.394e-27 Identity = 73/238 (30.67%), Postives = 119/238 (50.00%), Query Frame = 1 Query: 88 RKTKPHKKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFND----------SXXXXXXXXXXXXXXEGNPLTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNSLTNCEINSGKSI---ENLPASVEVRCELLDDGKYYFMSHAIE 762 +K P KK + V+ L ++ ++V+ ++N RT V IR++ D + + GK + + L + L+G LLFT+ + + E+L + S E+ R G +Q+V++ + PL F + P LR LGMPV++ GV+ L +DY +C + L+ R+LK F I+ +EF V + A+ D E+ S +L V V C+ LDDG YYF+ E Sbjct: 16 QKHAPKKKQHVAKVRQYLDEYKRVYVVTLHNPRTQKVSEIRKNMPDIKLLFGINKVTVLALGKTHKDSYRPKLHHLCKYLKGQCALLFTKSSPSELREQLDAFRSAEYCRPGAPAEQTVRIASGPLPKFPHTMEPVLRQLGMPVKLVRGVVHLERDYLVCNSEDVLSPEQCRILKLFQIEMSEFRVGLLAVWTDGDGVE-ELEEKDSCLMRTSLHPEVIVVCKQLDDGLYYFIPQPCE 252
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Cavefish
Match: mrto4 (MRT4 homolog, ribosome maturation factor [Source:NCBI gene;Acc:103032884]) HSP 1 Score: 107.071 bits (266), Expect = 1.929e-27 Identity = 61/190 (32.11%), Postives = 104/190 (54.74%), Query Frame = 1 Query: 106 KKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEGNP----------LTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNS 645 K+N I+ ++ + + +F+ ++ N+R N +K IR + S+ FGK K+ AL P +++ L G +LFT + E V E + +++AR+G + Q +V + PL+ F + P LR LG+P +K GV+ L++DY++CK+G+ L +R+LK F + AEF + IK M S Sbjct: 23 KQNLIEELRKCVDTYRYVFIFSVENMRNNKLKDIRAAWKHSRFFFGKNKVMMIALGKGPTDEYKDNLHRVSKFLRGEVGVLFTNKTKEEVQEYFNSFKEVDYARSGNVAQMAVTLDEGPLEQFTHSMEPQLRQLGLPTALKKGVVTLIKDYEVCKEGDTLTPEQARILKLFGFEMAEFKLSIKCMWNSES 212
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Cavefish
Match: mrto4 (MRT4 homolog, ribosome maturation factor [Source:NCBI gene;Acc:103032884]) HSP 1 Score: 107.071 bits (266), Expect = 2.371e-27 Identity = 61/190 (32.11%), Postives = 104/190 (54.74%), Query Frame = 1 Query: 106 KKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEGNP----------LTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNS 645 K+N I+ ++ + + +F+ ++ N+R N +K IR + S+ FGK K+ AL P +++ L G +LFT + E V E + +++AR+G + Q +V + PL+ F + P LR LG+P +K GV+ L++DY++CK+G+ L +R+LK F + AEF + IK M S Sbjct: 29 KQNLIEELRKCVDTYRYVFIFSVENMRNNKLKDIRAAWKHSRFFFGKNKVMMIALGKGPTDEYKDNLHRVSKFLRGEVGVLFTNKTKEEVQEYFNSFKEVDYARSGNVAQMAVTLDEGPLEQFTHSMEPQLRQLGLPTALKKGVVTLIKDYEVCKEGDTLTPEQARILKLFGFEMAEFKLSIKCMWNSES 218
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Yeast
Match: MRT4 (Protein involved in mRNA turnover and ribosome assembly; required at post-transcriptional step for efficient retrotransposition; localizes to the nucleolus [Source:SGD;Acc:S000001492]) HSP 1 Score: 64.6994 bits (156), Expect = 8.970e-13 Identity = 55/212 (25.94%), Postives = 107/212 (50.47%), Query Frame = 1 Query: 85 ERKTKPHKKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEGN----------PLTEVLEGNDVLLFTERNYENVSEELTKYTSLEFART------------GVLCQQSVKVLAQPLKVFDSGLAPTLRD-LGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNSLT 651 ++K + +K+ D V+ L + ++VL+++++RT +++ IR + SK++ GK+K+ KAL L+++ G LLFT+ + V E Y +++R G++ + ++ A+ L PT+R+ +P +IK G I + Y +C +GE+L+ + +LK F I +EF V++ A ++S T Sbjct: 16 DKKGRENKERIFDEVREALDTYRYVWVLHLDDVRTPVLQEIRTSWAGSKLIMGKRKVLQKALGEKREEEYKENLYQLSKLCSGVTGLLFTDEDVNTVKEYFKSYVRSDYSRPNTKAPLTFTIPEGIVYSRGGQIPAEEDVPMIHSLEPTMRNKFEIPTKIKAGKITIDSPYLVCTEGEKLDVRQALILKQFGIAASEFKVKVSAYYDNDSST 227
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Nematostella
Match: EDO30123 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T1P1]) HSP 1 Score: 89.3521 bits (220), Expect = 2.182e-21 Identity = 57/182 (31.32%), Postives = 98/182 (53.85%), Query Frame = 1 Query: 106 KKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEGNP-------LTEV---LEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEI 621 K + V+ + +++ ++V ++ N+R + +K RE + S+ FGK K+ AL P L EV L+G LLFT ++ ++V+ +Y+ ++FAR G ++ A PL F P LR LG+P +K GV+ L+ D+ +CK+G+ L +R+LK F AEF++ + Sbjct: 23 KSGLVKEVQECVDEYAFVYVFSVENMRNSKLKDAREKWKQSRFFFGKNKVMAIALGKTPEDEYKENLCEVSKRLKGQCGLLFTNQSKDDVTSWFKEYSEMDFARAGNKAICDFQLDAGPLDQFPHSTEPNLRQLGLPTALKKGVVTLLSDHTVCKEGDVLTPEQARILKLFGNPMAEFHITL 204
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Medaka
Match: mrto4 (MRT4 homolog, ribosome maturation factor [Source:NCBI gene;Acc:101175392]) HSP 1 Score: 104.76 bits (260), Expect = 1.407e-26 Identity = 57/186 (30.65%), Postives = 100/186 (53.76%), Query Frame = 1 Query: 106 KKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXX-XXXXXXXXXXXEGNP---------LTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAML 633 K+ I+ ++ + + +F+ ++ N+R N +K IR + S+ K + +G+P +++ L G +LFT + + V E + + ++FAR G Q V + PL+ F + P LR LG+P +K GV+ L++DY++CK+G+ L +R+LK F I+ AEF V+IK M Sbjct: 23 KQKLIEELRKCVDTYRNLFIFSVENMRNNKLKDIRTAWKHSRFFFGKNKVMIVALGKGSPDEYKDNLHKVSKFLRGEVGVLFTNKTKDEVYEYFSHFKEMDFARAGNQAQMDVTLDEGPLEQFPHSMEPQLRQLGLPTALKKGVVTLLKDYEVCKEGDILTPEQARILKLFAIEMAEFKVKIKCMW 208
BLAST of Ribosome assembly factor mrt4 vs. Planmine SMEST
Match: SMESG000020510.1 (SMESG000020510.1) HSP 1 Score: 437.187 bits (1123), Expect = 1.044e-156 Identity = 231/234 (98.72%), Postives = 231/234 (98.72%), Query Frame = 1 Query: 82 VERKTKPHKKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSXXXXXXXXXXXXXXEGNPLTEVLEGNDVLLFTERNYENVSEELTKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNSLTNCEINSGKSIENLPASVEVRCELLDDGKYYFMSHAIEEAAMDCE 783 VERKTKPHKKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSKILFGKKKLFFKALEGN LTEVLEGNDVLLFTERNYENVSEEL KYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNSLTN EINSGKSIENLPASVEVRCELLDDGKYYFMSHAIEEAAMDCE Sbjct: 18 VERKTKPHKKNFIDTVKTVLGQFSTIFVLNINNLRTNLVKRIREDFNDSKILFGKKKLFFKALEGNHLTEVLEGNDVLLFTERNYENVSEELAKYTSLEFARTGVLCQQSVKVLAQPLKVFDSGLAPTLRDLGMPVEIKDGVIILMQDYQICKKGEQLNNSMSRLLKFFNIQTAEFNVEIKAMLKDNSLTNYEINSGKSIENLPASVEVRCELLDDGKYYFMSHAIEEAAMDCE 251 The following BLAST results are available for this feature:
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 1
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of Ribosome assembly factor mrt4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Ribosome assembly factor mrt4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 2
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Ribosome assembly factor mrt4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of Ribosome assembly factor mrt4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30013497 ID=SMED30013497|Name=Ribosome assembly factor mrt4|organism=Schmidtea mediterranea sexual|type=transcript|length=855bpback to top protein sequence of SMED30013497-orf-1 >SMED30013497-orf-1 ID=SMED30013497-orf-1|Name=SMED30013497-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=252bp MVKSKKISKTKKQVNTKVERKTKPHKKNFIDTVKTVLGQFSTIFVLNINNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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