SMED30013443

Overview
NameSMED30013443
Smed IDSMED30013443
Length (bp)414
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30013443 (SMED30013443) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30013443 (SMED30013443) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013443

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30013443h1SMcG0010684 SmedASXL_019870SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30013443h1SMcG0002131 OX_Smed_1.0.02541ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30013443h1SMcG0002130 OX_Smed_1.0.02541ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30013443h1SMcG0010683 OX_Smed_1.0.02541ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30013443h1SMcG0010684 OX_Smed_1.0.02541ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
gutSMED30013443h1SMcG0010684 SMED30024934smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30013443h1SMcG0010684 SMED30024934smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30013443h1SMcG0010683 SMED30024934smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30013443h1SMcG0010683 SMED30024934smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30013443 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001226:49729..50870 +2049
Homology
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BLAST of SMED30013443 vs. Planmine SMEST
Match: SMESG000039796.1 (SMESG000039796.1)

HSP 1 Score: 233.417 bits (594), Expect = 1.491e-77
Identity = 113/114 (99.12%), Postives = 113/114 (99.12%), Query Frame = 2 
Query:    2 GIPVITIKPQAYKTSILFSTNEVFGANETNLYGEKFIKKSEDILQNWIEGASENFHEVINTLESATLNRTYQYKYIPNFIFRMKLLMLNFNSTSNLTRTGFQNGDISIPVKTEF 343
            GIPVITIKPQAYKTSILFSTNEVFGANETNLYGEKFIKKSEDILQNWIEGASENFHEVINTLESA LNRTYQYKYIPNFIFRMKLLMLNFNSTSNLTRTGFQNGDISIPVKTEF
Sbjct:  235 GIPVITIKPQAYKTSILFSTNEVFGANETNLYGEKFIKKSEDILQNWIEGASENFHEVINTLESAILNRTYQYKYIPNFIFRMKLLMLNFNSTSNLTRTGFQNGDISIPVKTEF 348
[Close]
BLAST of SMED30013443 vs. Planmine SMEST
Match: SMESG000039796.1 (SMESG000039796.1)

HSP 1 Score: 233.802 bits (595), Expect = 3.493e-75
Identity = 113/114 (99.12%), Postives = 113/114 (99.12%), Query Frame = 2 
Query:    2 GIPVITIKPQAYKTSILFSTNEVFGANETNLYGEKFIKKSEDILQNWIEGASENFHEVINTLESATLNRTYQYKYIPNFIFRMKLLMLNFNSTSNLTRTGFQNGDISIPVKTEF 343
            GIPVITIKPQAYKTSILFSTNEVFGANETNLYGEKFIKKSEDILQNWIEGASENFHEVINTLESA LNRTYQYKYIPNFIFRMKLLMLNFNSTSNLTRTGFQNGDISIPVKTEF
Sbjct:  453 GIPVITIKPQAYKTSILFSTNEVFGANETNLYGEKFIKKSEDILQNWIEGASENFHEVINTLESAILNRTYQYKYIPNFIFRMKLLMLNFNSTSNLTRTGFQNGDISIPVKTEF 566
[Close]
BLAST of SMED30013443 vs. Planmine SMEST
Match: SMESG000039794.1 (SMESG000039794.1)

HSP 1 Score: 180.644 bits (457), Expect = 7.721e-58
Identity = 87/114 (76.32%), Postives = 95/114 (83.33%), Query Frame = 2 
Query:    2 GIPVITIKPQAYKTSILFSTNEVFGANETNLYGEKFIKKSEDILQNWIEGASENFHEVINTLESATLNRTYQYKYIPNFIFRMKLLMLNFNSTSNLTRTGFQNGDISIPVKTEF 343
            GIPVITI PQAYKT IL STN+VFG  ET LYGEKFIKKSEDIL+NW+E AS NF EVIN LESA LNRTYQYKY PNF+FRMK+LM+  NS +N  RTGF+  DISIPVKTEF
Sbjct:  146 GIPVITINPQAYKTYILLSTNKVFGTEETKLYGEKFIKKSEDILRNWVESASVNFQEVINALESAILNRTYQYKYKPNFVFRMKILMMKANSLNNRIRTGFRYSDISIPVKTEF 259
[Close]
BLAST of SMED30013443 vs. Planmine SMEST
Match: SMESG000039792.1 (SMESG000039792.1)

HSP 1 Score: 181.8 bits (460), Expect = 7.906e-57
Identity = 87/114 (76.32%), Postives = 95/114 (83.33%), Query Frame = 2 
Query:    2 GIPVITIKPQAYKTSILFSTNEVFGANETNLYGEKFIKKSEDILQNWIEGASENFHEVINTLESATLNRTYQYKYIPNFIFRMKLLMLNFNSTSNLTRTGFQNGDISIPVKTEF 343
            GIPVITI PQAYKT IL STN+VFG  ET LYGEKFIKKSEDIL+NW+E AS NF EVIN LESA LNRTYQYKY PNF+FRMK+LM+  NS +N  RTGF+  DISIPVKTEF
Sbjct:  274 GIPVITINPQAYKTYILLSTNKVFGTEETKLYGEKFIKKSEDILRNWVESASVNFQEVINALESAILNRTYQYKYKPNFVFRMKILMMKANSLNNRIRTGFRYSDISIPVKTEF 387
[Close]
BLAST of SMED30013443 vs. Planmine SMEST
Match: SMESG000039792.1 (SMESG000039792.1)

HSP 1 Score: 180.644 bits (457), Expect = 8.648e-57
Identity = 87/114 (76.32%), Postives = 95/114 (83.33%), Query Frame = 2 
Query:    2 GIPVITIKPQAYKTSILFSTNEVFGANETNLYGEKFIKKSEDILQNWIEGASENFHEVINTLESATLNRTYQYKYIPNFIFRMKLLMLNFNSTSNLTRTGFQNGDISIPVKTEF 343
            GIPVITI PQAYKT IL STN+VFG  ET LYGEKFIKKSEDIL+NW+E AS NF EVIN LESA LNRTYQYKY PNF+FRMK+LM+  NS +N  RTGF+  DISIPVKTEF
Sbjct:  235 GIPVITINPQAYKTYILLSTNKVFGTEETKLYGEKFIKKSEDILRNWVESASVNFQEVINALESAILNRTYQYKYKPNFVFRMKILMMKANSLNNRIRTGFRYSDISIPVKTEF 348
The following BLAST results are available for this feature:
BLAST of SMED30013443 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013443 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013443 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013443 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013443 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013443 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013443 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013443 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013443 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013443 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013443 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013443 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013443 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013443 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000039796.11.491e-7799.12SMESG000039796.1[more]
SMESG000039796.13.493e-7599.12SMESG000039796.1[more]
SMESG000039794.17.721e-5876.32SMESG000039794.1[more]
SMESG000039792.17.906e-5776.32SMESG000039792.1[more]
SMESG000039792.18.648e-5776.32SMESG000039792.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013443 ID=SMED30013443|Name=SMED30013443|organism=Schmidtea mediterranea sexual|type=transcript|length=414bp
GGGAATACCCGTTATTACTATTAAACCACAAGCTTACAAAACTTCTATTC
TATTTTCAACTAATGAAGTTTTCGGAGCCAACGAAACTAATTTATACGGA
GAGAAGTTCATTAAAAAGTCCGAAGACATTCTTCAAAACTGGATTGAGGG
CGCCAGTGAAAACTTCCACGAAGTGATCAATACATTGGAGAGTGCTACTT
TGAACCGGACTTATCAATACAAATACATACCGAATTTTATTTTTAGAATG
AAGCTTTTGATGCTGAATTTCAATTCGACCTCAAATCTGACCCGGACCGG
ATTTCAAAATGGCGACATTTCTATACCAGTAAAAACGGAATTTTGATCTC
TTTTCATTTCACGTTGACTTGATGTTTACGAAGTGATTTTGTTTACATAA
ATATTTATGGAAAA
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protein sequence of SMED30013443-orf-1

>SMED30013443-orf-1 ID=SMED30013443-orf-1|Name=SMED30013443-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=115bp
GIPVITIKPQAYKTSILFSTNEVFGANETNLYGEKFIKKSEDILQNWIEG
ASENFHEVINTLESATLNRTYQYKYIPNFIFRMKLLMLNFNSTSNLTRTG
FQNGDISIPVKTEF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000420parapharyngeal region