Vigilin High density lipoprotein-binding protein
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30013430 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Human
Match: HDLBP (high density lipoprotein binding protein [Source:HGNC Symbol;Acc:HGNC:4857]) HSP 1 Score: 326.635 bits (836), Expect = 2.889e-112 Identity = 214/687 (31.15%), Postives = 384/687 (55.90%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQ-KSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
K +H N + +++ R++IPP SV++ EI +G+++ +AQA + + + Y + K + +E+ K +HKY+IGP+G+ ++EIL++T V V+I ++ T+ L G + LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V E ++ V DL +R+ +E+ ID +H+IG G NI +I+ + S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+D+FPEV INFP P SD++ L GP+ +V++ +QK A LVE+++S V I ++HK IIG+ G I+++ + +NTK +PA + ++ I+ITGK+ + E A R+ +I + +NI E+ + I LH LIG G I S+ E CGG V I FP + S+TV+I+G DV KA+ +L L EE ++ + E ++FL K++K+R++ R+ +E +D+ + IIG+++ V + L+ + +V +++ + +F + ++ R + + V +S + ++ + +G +++AK I II+D E +T + ++ +++ +++ +I++ + VQI+ P
Sbjct: 231 KAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPP---SDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVAAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGG-VHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFP 910 HSP 2 Score: 101.679 bits (252), Expect = 2.889e-112 Identity = 54/134 (40.30%), Postives = 79/134 (58.96%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHAR 497
IT F +P EER+Y D + K+ EI G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G ++LE T+T++ P+ +DPS IKI G+K+GIE AR
Sbjct: 76 ITQVFHVPLEERKYKDMNQFGEGEQAKICLEIMQRTGAHLELSLAKDQGLSIMVSGKLDAVMKARKDIVARLQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKAR 209 HSP 3 Score: 156.762 bits (395), Expect = 7.700e-38 Identity = 97/311 (31.19%), Postives = 173/311 (55.63%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN---INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQ 3826
I+G K+K AKE + L + +EV VP + + +IG KG I+K+ +V+I +P + + +TG + ++ AK ++E V+EL+ +++R +R+F L++ V +YH K+IG KG I++IR ++ V I P +D ++ITI G++ N E A++ +L V +LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N V V G E+V+ D +++ L ++ D L+ Y EE P++ GF +R+APW A ++ S+S++
Sbjct: 953 ISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSG--APDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQ-----VYMKPPAHEEAKAPSR-------GFVVRDAPWTASSSEKAPDMSSSEE 1249 HSP 4 Score: 94.3597 bits (233), Expect = 2.942e-27 Identity = 116/512 (22.66%), Postives = 218/512 (42.58%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDA----PGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQ---IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP---------------------------RDLTK------KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQV-AAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEEN---DEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDA-DSNTVIIKGVKDDVLKAQVKLHDLVEEITA 2053
+ I G V +A LL + + + I E HK++IG G I+++ D T V + + I ++G +D + A + ++++ + D+ P F+I + G L +++ + + F Q + ++G+ + V + +++ + DL+++++ LE I + ++G G IQ+I ++ + I FP +D ++ I G EA K L ++ L +IGQ+G G++++ D F EV I+ P P+ SD+I++ G ++D+ A +L++ E +F V + KYH IIGR+G I ++ + Q P + N D+I ITG +K+ E A + I + ++ + + +H +IG G I + + +V IRFP A D N V + G+ ++V +A + +L EE A
Sbjct: 700 VVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPA--AEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVI-RRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-LECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEAL-----EALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEF-EVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF--KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLA 1199 HSP 5 Score: 49.6766 bits (117), Expect = 2.942e-27 Identity = 39/139 (28.06%), Postives = 69/139 (49.64%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITR----LVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIFLHF 653
V + G K++V + KY+ + LV+ + IS+ I K+FH+++IG G+ K++ ++T++ P N S I I G + E AR I + + I V I + + N L+ RL ++E + +HF
Sbjct: 554 VQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEE--CGGVHIHF 690 HSP 6 Score: 113.235 bits (282), Expect = 2.150e-24 Identity = 152/689 (22.06%), Postives = 300/689 (43.54%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALS-INDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLI-VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSSTISAGVALN-KDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDL-----EVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
+++ S V IP ++H+ +IG+ G +++L T TK QIP + +++I ITG ++ +E A V I ++ +E+ K H + G + + + G R+ I P P + ++ G K+ + +A ++ + EE ++ ++++ L+++ R SV I ++ S I+ G+ E + L EV KA S V+ A S ++ +K L +I + M K+++ + + + GP +++ A+++I ++ D N + I +D + +++ S ++ I +Y+V ++IP E N I + ++ + N D++ E + G K I EI + I +N DP + Q + G K+++ + + K+ V DL ++VP ++F+ +IG G IKK+R I +P +NS +I+TG + AA+ I+ ++L + + + + +PA+ H LIG KG I I + V I P + S + + I+G +VEKAK ++L + ++ V + H LIG G + +++ ++FP E ++ +++ + G +++V EL
Sbjct: 147 LQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINI--PPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFT---VSSVAAPSWLHRFIIGKKGQNLAKITQ-QMPKVHIEFTEGEDK------ITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRME-------------NERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVI-----INFPDPAQKSDIVQLR----GPKNEVEKCTKYMQKM-VADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAE--------VEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFP--AAEDKDQDLITIIGKEDAVREAQKEL 790 HSP 7 Score: 109.383 bits (272), Expect = 2.673e-23 Identity = 141/643 (21.93%), Postives = 286/643 (44.48%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK-SNELF----RIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDER 2521
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++++ +T+ + I R ++ S N I + G K+ + A + L+ + + + ++ H FI G ++G+ N+ SVN+ +IV G E++ + +KK + + + + + +E+ K++IG G ++Q+I + T S+ P D + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP +DV+ ++ L+ ++ I K+H+ +IG+ G I + +IP SE+++ I I G + V+ A + + + + N + I + H +IG+ G RI +++ V I FP P S+ V ++G K++V K + +V ++ ++Y + + ++ + +KK+RE + +I +E + + I G++ N + ARS + I + ++ +++ K N L R+ R M + + + + RGP +++ AK +++H+ ++ + T++ VD R
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQTQASAT-----VAIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPDDPS-NQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR-----------TEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVAAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKF-PEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANC-------EAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQ---TKSFTVDIR 733 HSP 8 Score: 77.0258 bits (188), Expect = 1.335e-20 Identity = 98/450 (21.78%), Postives = 196/450 (43.56%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNE--------------------------GSP---NTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR----DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDA-DSDLISLIGPRKDVDQ-VAAILQKKHAFLVESNFSKKVKI---PFKYHKAIIGREGITIRELTKATNTKFQIP--AASEE 1744
K + + E + + P +++++ G +D V A + + ++A L+ IP++ H+ ++GP+GS I++I SV + ++ N GSP + I + G K+ A + ++ I ++ P LH+++IG+ GS + V++ V P +Q ++ I I G + L ++V +LQ+ R +L + +DP IIG G I +IR E + +I FP + + +TI G EA + +L+ + +E +D+ H + ++IG +GK +++I D F +V I FP A D + +++ G ++V++ + IL + +L + S+ +++ P + +A G +R+ ++ + P ++SEE
Sbjct: 810 KHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-LECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEAL--EALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQ------SDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF-KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQVYMKPPAHEEAKAPSRGFVVRDAPWTASSSEKAPDMSSSEE 1249 HSP 9 Score: 44.669 bits (104), Expect = 1.335e-20 Identity = 33/118 (27.97%), Postives = 56/118 (47.46%), Query Frame = 3
Query: 174 KLLTEICANCG-VRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQ----FEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPST-LIKIKGSKDGIEHARQLIQ 509
+L+ I CG V + V S V I G V +AKK + L + + F + + E+H+ LIG G +++ ++T +V FP D LI I G +D + A++ ++
Sbjct: 674 RLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELE 791 HSP 10 Score: 82.8037 bits (203), Expect = 3.794e-15 Identity = 59/218 (27.06%), Postives = 121/218 (55.50%), Query Frame = 2
Query: 2981 VPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRA 3625
+ + F+ +IG KGE+I+++R +V I P+ K V + G K E+ + + +++M + ++S+++ + Q+H+ +IG G I KIR +++ +I +P+++SN I I G + N E A++ +L+ L + I EV ++I +++H+ LIG++G + + ++ V I FP+ EG ++ V ++G V++ +L+ A
Sbjct: 514 IEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGP-------KNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDL-ANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPV---EGSGSDTVVIRGPSSDVEKAKKQLLHLA 720 HSP 11 Score: 73.9442 bits (180), Expect = 1.892e-12 Identity = 62/286 (21.68%), Postives = 126/286 (44.06%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELV 3616
K+ + G KD + AK+ I ++ + LE +P++F+ +++G KG +I+++ V IK P++ + +I++G E AAK+ + V ++ + VP HR +IG KG I K+ + V I VP+ + + I I G N+++AK +L V +L++ + +D + H ++IG +GA + Q++ ++ V I FP + + + + + G +++ + D ++
Sbjct: 846 KVTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEALEALVP-------------VTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAIL 1118 HSP 12 Score: 62.003 bits (149), Expect = 9.616e-9 Identity = 34/124 (27.42%), Postives = 65/124 (52.42%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIG 3469
++V+G ++V+ A+K+IV ++ S + +P ++HR +IG GE + + K + +I +P D N+I I G K+ +EKA++EVL + + +E L ++ H + G
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQT-----------QASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAG 239
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Human
Match: HDLBP (high density lipoprotein binding protein [Source:HGNC Symbol;Acc:HGNC:4857]) HSP 1 Score: 326.635 bits (836), Expect = 2.889e-112 Identity = 214/687 (31.15%), Postives = 384/687 (55.90%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQ-KSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
K +H N + +++ R++IPP SV++ EI +G+++ +AQA + + + Y + K + +E+ K +HKY+IGP+G+ ++EIL++T V V+I ++ T+ L G + LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V E ++ V DL +R+ +E+ ID +H+IG G NI +I+ + S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+D+FPEV INFP P SD++ L GP+ +V++ +QK A LVE+++S V I ++HK IIG+ G I+++ + +NTK +PA + ++ I+ITGK+ + E A R+ +I + +NI E+ + I LH LIG G I S+ E CGG V I FP + S+TV+I+G DV KA+ +L L EE ++ + E ++FL K++K+R++ R+ +E +D+ + IIG+++ V + L+ + +V +++ + +F + ++ R + + V +S + ++ + +G +++AK I II+D E +T + ++ +++ +++ +I++ + VQI+ P
Sbjct: 231 KAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPP---SDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVAAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGG-VHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFP 910 HSP 2 Score: 101.679 bits (252), Expect = 2.889e-112 Identity = 54/134 (40.30%), Postives = 79/134 (58.96%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHAR 497
IT F +P EER+Y D + K+ EI G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G ++LE T+T++ P+ +DPS IKI G+K+GIE AR
Sbjct: 76 ITQVFHVPLEERKYKDMNQFGEGEQAKICLEIMQRTGAHLELSLAKDQGLSIMVSGKLDAVMKARKDIVARLQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKAR 209 HSP 3 Score: 156.762 bits (395), Expect = 7.700e-38 Identity = 97/311 (31.19%), Postives = 173/311 (55.63%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN---INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQ 3826
I+G K+K AKE + L + +EV VP + + +IG KG I+K+ +V+I +P + + +TG + ++ AK ++E V+EL+ +++R +R+F L++ V +YH K+IG KG I++IR ++ V I P +D ++ITI G++ N E A++ +L V +LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N V V G E+V+ D +++ L ++ D L+ Y EE P++ GF +R+APW A ++ S+S++
Sbjct: 953 ISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSG--APDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQ-----VYMKPPAHEEAKAPSR-------GFVVRDAPWTASSSEKAPDMSSSEE 1249 HSP 4 Score: 94.3597 bits (233), Expect = 2.942e-27 Identity = 116/512 (22.66%), Postives = 218/512 (42.58%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDA----PGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQ---IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP---------------------------RDLTK------KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQV-AAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEEN---DEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDA-DSNTVIIKGVKDDVLKAQVKLHDLVEEITA 2053
+ I G V +A LL + + + I E HK++IG G I+++ D T V + + I ++G +D + A + ++++ + D+ P F+I + G L +++ + + F Q + ++G+ + V + +++ + DL+++++ LE I + ++G G IQ+I ++ + I FP +D ++ I G EA K L ++ L +IGQ+G G++++ D F EV I+ P P+ SD+I++ G ++D+ A +L++ E +F V + KYH IIGR+G I ++ + Q P + N D+I ITG +K+ E A + I + ++ + + +H +IG G I + + +V IRFP A D N V + G+ ++V +A + +L EE A
Sbjct: 700 VVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPA--AEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVI-RRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-LECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEAL-----EALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEF-EVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF--KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLA 1199 HSP 5 Score: 49.6766 bits (117), Expect = 2.942e-27 Identity = 39/139 (28.06%), Postives = 69/139 (49.64%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITR----LVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIFLHF 653
V + G K++V + KY+ + LV+ + IS+ I K+FH+++IG G+ K++ ++T++ P N S I I G + E AR I + + I V I + + N L+ RL ++E + +HF
Sbjct: 554 VQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEE--CGGVHIHF 690 HSP 6 Score: 113.235 bits (282), Expect = 2.150e-24 Identity = 152/689 (22.06%), Postives = 300/689 (43.54%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALS-INDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLI-VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSSTISAGVALN-KDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDL-----EVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
+++ S V IP ++H+ +IG+ G +++L T TK QIP + +++I ITG ++ +E A V I ++ +E+ K H + G + + + G R+ I P P + ++ G K+ + +A ++ + EE ++ ++++ L+++ R SV I ++ S I+ G+ E + L EV KA S V+ A S ++ +K L +I + M K+++ + + + GP +++ A+++I ++ D N + I +D + +++ S ++ I +Y+V ++IP E N I + ++ + N D++ E + G K I EI + I +N DP + Q + G K+++ + + K+ V DL ++VP ++F+ +IG G IKK+R I +P +NS +I+TG + AA+ I+ ++L + + + + +PA+ H LIG KG I I + V I P + S + + I+G +VEKAK ++L + ++ V + H LIG G + +++ ++FP E ++ +++ + G +++V EL
Sbjct: 147 LQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINI--PPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFT---VSSVAAPSWLHRFIIGKKGQNLAKITQ-QMPKVHIEFTEGEDK------ITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRME-------------NERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVI-----INFPDPAQKSDIVQLR----GPKNEVEKCTKYMQKM-VADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAE--------VEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFP--AAEDKDQDLITIIGKEDAVREAQKEL 790 HSP 7 Score: 109.383 bits (272), Expect = 2.673e-23 Identity = 141/643 (21.93%), Postives = 286/643 (44.48%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK-SNELF----RIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDER 2521
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++++ +T+ + I R ++ S N I + G K+ + A + L+ + + + ++ H FI G ++G+ N+ SVN+ +IV G E++ + +KK + + + + + +E+ K++IG G ++Q+I + T S+ P D + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP +DV+ ++ L+ ++ I K+H+ +IG+ G I + +IP SE+++ I I G + V+ A + + + + N + I + H +IG+ G RI +++ V I FP P S+ V ++G K++V K + +V ++ ++Y + + ++ + +KK+RE + +I +E + + I G++ N + ARS + I + ++ +++ K N L R+ R M + + + + RGP +++ AK +++H+ ++ + T++ VD R
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQTQASAT-----VAIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPDDPS-NQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR-----------TEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVAAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKF-PEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANC-------EAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQ---TKSFTVDIR 733 HSP 8 Score: 77.0258 bits (188), Expect = 1.335e-20 Identity = 98/450 (21.78%), Postives = 196/450 (43.56%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNE--------------------------GSP---NTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR----DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDA-DSDLISLIGPRKDVDQ-VAAILQKKHAFLVESNFSKKVKI---PFKYHKAIIGREGITIRELTKATNTKFQIP--AASEE 1744
K + + E + + P +++++ G +D V A + + ++A L+ IP++ H+ ++GP+GS I++I SV + ++ N GSP + I + G K+ A + ++ I ++ P LH+++IG+ GS + V++ V P +Q ++ I I G + L ++V +LQ+ R +L + +DP IIG G I +IR E + +I FP + + +TI G EA + +L+ + +E +D+ H + ++IG +GK +++I D F +V I FP A D + +++ G ++V++ + IL + +L + S+ +++ P + +A G +R+ ++ + P ++SEE
Sbjct: 810 KHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-LECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEAL--EALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQ------SDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF-KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQVYMKPPAHEEAKAPSRGFVVRDAPWTASSSEKAPDMSSSEE 1249 HSP 9 Score: 44.669 bits (104), Expect = 1.335e-20 Identity = 33/118 (27.97%), Postives = 56/118 (47.46%), Query Frame = 3
Query: 174 KLLTEICANCG-VRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQ----FEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPST-LIKIKGSKDGIEHARQLIQ 509
+L+ I CG V + V S V I G V +AKK + L + + F + + E+H+ LIG G +++ ++T +V FP D LI I G +D + A++ ++
Sbjct: 674 RLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELE 791 HSP 10 Score: 82.8037 bits (203), Expect = 3.794e-15 Identity = 59/218 (27.06%), Postives = 121/218 (55.50%), Query Frame = 2
Query: 2981 VPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRA 3625
+ + F+ +IG KGE+I+++R +V I P+ K V + G K E+ + + +++M + ++S+++ + Q+H+ +IG G I KIR +++ +I +P+++SN I I G + N E A++ +L+ L + I EV ++I +++H+ LIG++G + + ++ V I FP+ EG ++ V ++G V++ +L+ A
Sbjct: 514 IEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGP-------KNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDL-ANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPV---EGSGSDTVVIRGPSSDVEKAKKQLLHLA 720 HSP 11 Score: 73.9442 bits (180), Expect = 1.892e-12 Identity = 62/286 (21.68%), Postives = 126/286 (44.06%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELV 3616
K+ + G KD + AK+ I ++ + LE +P++F+ +++G KG +I+++ V IK P++ + +I++G E AAK+ + V ++ + VP HR +IG KG I K+ + V I VP+ + + I I G N+++AK +L V +L++ + +D + H ++IG +GA + Q++ ++ V I FP + + + + + G +++ + D ++
Sbjct: 846 KVTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEALEALVP-------------VTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAIL 1118 HSP 12 Score: 62.003 bits (149), Expect = 9.616e-9 Identity = 34/124 (27.42%), Postives = 65/124 (52.42%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIG 3469
++V+G ++V+ A+K+IV ++ S + +P ++HR +IG GE + + K + +I +P D N+I I G K+ +EKA++EVL + + +E L ++ H + G
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQT-----------QASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAG 239
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Human
Match: HDLBP (high density lipoprotein binding protein [Source:HGNC Symbol;Acc:HGNC:4857]) HSP 1 Score: 326.635 bits (836), Expect = 2.889e-112 Identity = 214/687 (31.15%), Postives = 384/687 (55.90%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQ-KSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
K +H N + +++ R++IPP SV++ EI +G+++ +AQA + + + Y + K + +E+ K +HKY+IGP+G+ ++EIL++T V V+I ++ T+ L G + LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V E ++ V DL +R+ +E+ ID +H+IG G NI +I+ + S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+D+FPEV INFP P SD++ L GP+ +V++ +QK A LVE+++S V I ++HK IIG+ G I+++ + +NTK +PA + ++ I+ITGK+ + E A R+ +I + +NI E+ + I LH LIG G I S+ E CGG V I FP + S+TV+I+G DV KA+ +L L EE ++ + E ++FL K++K+R++ R+ +E +D+ + IIG+++ V + L+ + +V +++ + +F + ++ R + + V +S + ++ + +G +++AK I II+D E +T + ++ +++ +++ +I++ + VQI+ P
Sbjct: 231 KAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPP---SDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVAAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGG-VHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFP 910 HSP 2 Score: 101.679 bits (252), Expect = 2.889e-112 Identity = 54/134 (40.30%), Postives = 79/134 (58.96%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHAR 497
IT F +P EER+Y D + K+ EI G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G ++LE T+T++ P+ +DPS IKI G+K+GIE AR
Sbjct: 76 ITQVFHVPLEERKYKDMNQFGEGEQAKICLEIMQRTGAHLELSLAKDQGLSIMVSGKLDAVMKARKDIVARLQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKAR 209 HSP 3 Score: 156.762 bits (395), Expect = 7.700e-38 Identity = 97/311 (31.19%), Postives = 173/311 (55.63%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN---INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQ 3826
I+G K+K AKE + L + +EV VP + + +IG KG I+K+ +V+I +P + + +TG + ++ AK ++E V+EL+ +++R +R+F L++ V +YH K+IG KG I++IR ++ V I P +D ++ITI G++ N E A++ +L V +LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N V V G E+V+ D +++ L ++ D L+ Y EE P++ GF +R+APW A ++ S+S++
Sbjct: 953 ISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSG--APDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQ-----VYMKPPAHEEAKAPSR-------GFVVRDAPWTASSSEKAPDMSSSEE 1249 HSP 4 Score: 94.3597 bits (233), Expect = 2.942e-27 Identity = 116/512 (22.66%), Postives = 218/512 (42.58%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDA----PGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQ---IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP---------------------------RDLTK------KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQV-AAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEEN---DEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDA-DSNTVIIKGVKDDVLKAQVKLHDLVEEITA 2053
+ I G V +A LL + + + I E HK++IG G I+++ D T V + + I ++G +D + A + ++++ + D+ P F+I + G L +++ + + F Q + ++G+ + V + +++ + DL+++++ LE I + ++G G IQ+I ++ + I FP +D ++ I G EA K L ++ L +IGQ+G G++++ D F EV I+ P P+ SD+I++ G ++D+ A +L++ E +F V + KYH IIGR+G I ++ + Q P + N D+I ITG +K+ E A + I + ++ + + +H +IG G I + + +V IRFP A D N V + G+ ++V +A + +L EE A
Sbjct: 700 VVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPA--AEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVI-RRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-LECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEAL-----EALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEF-EVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF--KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLA 1199 HSP 5 Score: 49.6766 bits (117), Expect = 2.942e-27 Identity = 39/139 (28.06%), Postives = 69/139 (49.64%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITR----LVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIFLHF 653
V + G K++V + KY+ + LV+ + IS+ I K+FH+++IG G+ K++ ++T++ P N S I I G + E AR I + + I V I + + N L+ RL ++E + +HF
Sbjct: 554 VQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEE--CGGVHIHF 690 HSP 6 Score: 113.235 bits (282), Expect = 2.150e-24 Identity = 152/689 (22.06%), Postives = 300/689 (43.54%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALS-INDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLI-VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSSTISAGVALN-KDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDL-----EVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
+++ S V IP ++H+ +IG+ G +++L T TK QIP + +++I ITG ++ +E A V I ++ +E+ K H + G + + + G R+ I P P + ++ G K+ + +A ++ + EE ++ ++++ L+++ R SV I ++ S I+ G+ E + L EV KA S V+ A S ++ +K L +I + M K+++ + + + GP +++ A+++I ++ D N + I +D + +++ S ++ I +Y+V ++IP E N I + ++ + N D++ E + G K I EI + I +N DP + Q + G K+++ + + K+ V DL ++VP ++F+ +IG G IKK+R I +P +NS +I+TG + AA+ I+ ++L + + + + +PA+ H LIG KG I I + V I P + S + + I+G +VEKAK ++L + ++ V + H LIG G + +++ ++FP E ++ +++ + G +++V EL
Sbjct: 147 LQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINI--PPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFT---VSSVAAPSWLHRFIIGKKGQNLAKITQ-QMPKVHIEFTEGEDK------ITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRME-------------NERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVI-----INFPDPAQKSDIVQLR----GPKNEVEKCTKYMQKM-VADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAE--------VEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFP--AAEDKDQDLITIIGKEDAVREAQKEL 790 HSP 7 Score: 109.383 bits (272), Expect = 2.673e-23 Identity = 141/643 (21.93%), Postives = 286/643 (44.48%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK-SNELF----RIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDER 2521
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++++ +T+ + I R ++ S N I + G K+ + A + L+ + + + ++ H FI G ++G+ N+ SVN+ +IV G E++ + +KK + + + + + +E+ K++IG G ++Q+I + T S+ P D + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP +DV+ ++ L+ ++ I K+H+ +IG+ G I + +IP SE+++ I I G + V+ A + + + + N + I + H +IG+ G RI +++ V I FP P S+ V ++G K++V K + +V ++ ++Y + + ++ + +KK+RE + +I +E + + I G++ N + ARS + I + ++ +++ K N L R+ R M + + + + RGP +++ AK +++H+ ++ + T++ VD R
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQTQASAT-----VAIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPDDPS-NQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR-----------TEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVAAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKF-PEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANC-------EAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQ---TKSFTVDIR 733 HSP 8 Score: 77.0258 bits (188), Expect = 1.335e-20 Identity = 98/450 (21.78%), Postives = 196/450 (43.56%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNE--------------------------GSP---NTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR----DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDA-DSDLISLIGPRKDVDQ-VAAILQKKHAFLVESNFSKKVKI---PFKYHKAIIGREGITIRELTKATNTKFQIP--AASEE 1744
K + + E + + P +++++ G +D V A + + ++A L+ IP++ H+ ++GP+GS I++I SV + ++ N GSP + I + G K+ A + ++ I ++ P LH+++IG+ GS + V++ V P +Q ++ I I G + L ++V +LQ+ R +L + +DP IIG G I +IR E + +I FP + + +TI G EA + +L+ + +E +D+ H + ++IG +GK +++I D F +V I FP A D + +++ G ++V++ + IL + +L + S+ +++ P + +A G +R+ ++ + P ++SEE
Sbjct: 810 KHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-LECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEAL--EALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQ------SDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF-KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQVYMKPPAHEEAKAPSRGFVVRDAPWTASSSEKAPDMSSSEE 1249 HSP 9 Score: 44.669 bits (104), Expect = 1.335e-20 Identity = 33/118 (27.97%), Postives = 56/118 (47.46%), Query Frame = 3
Query: 174 KLLTEICANCG-VRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQ----FEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPST-LIKIKGSKDGIEHARQLIQ 509
+L+ I CG V + V S V I G V +AKK + L + + F + + E+H+ LIG G +++ ++T +V FP D LI I G +D + A++ ++
Sbjct: 674 RLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELE 791 HSP 10 Score: 82.8037 bits (203), Expect = 3.794e-15 Identity = 59/218 (27.06%), Postives = 121/218 (55.50%), Query Frame = 2
Query: 2981 VPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRA 3625
+ + F+ +IG KGE+I+++R +V I P+ K V + G K E+ + + +++M + ++S+++ + Q+H+ +IG G I KIR +++ +I +P+++SN I I G + N E A++ +L+ L + I EV ++I +++H+ LIG++G + + ++ V I FP+ EG ++ V ++G V++ +L+ A
Sbjct: 514 IEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGP-------KNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDL-ANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPV---EGSGSDTVVIRGPSSDVEKAKKQLLHLA 720 HSP 11 Score: 73.9442 bits (180), Expect = 1.892e-12 Identity = 62/286 (21.68%), Postives = 126/286 (44.06%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELV 3616
K+ + G KD + AK+ I ++ + LE +P++F+ +++G KG +I+++ V IK P++ + +I++G E AAK+ + V ++ + VP HR +IG KG I K+ + V I VP+ + + I I G N+++AK +L V +L++ + +D + H ++IG +GA + Q++ ++ V I FP + + + + + G +++ + D ++
Sbjct: 846 KVTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEALEALVP-------------VTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAIL 1118 HSP 12 Score: 62.003 bits (149), Expect = 9.616e-9 Identity = 34/124 (27.42%), Postives = 65/124 (52.42%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIG 3469
++V+G ++V+ A+K+IV ++ S + +P ++HR +IG GE + + K + +I +P D N+I I G K+ +EKA++EVL + + +E L ++ H + G
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQT-----------QASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAG 239
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Human
Match: HDLBP (high density lipoprotein binding protein [Source:HGNC Symbol;Acc:HGNC:4857]) HSP 1 Score: 323.553 bits (828), Expect = 4.687e-91 Identity = 276/992 (27.82%), Postives = 488/992 (49.19%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAA-SILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFT----KSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQ-KSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP-------FYPEPV-----------------NSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEIN-----SEVSSTISA--GVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNK---NSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQY--HRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVL 3391
K +H+ K+ L + + + ++ IP N+I I+G ++G+ +A +LL + Q K + L++E K H +I GP + EI+ +T ++I +N I G K+ L A+ + K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V E ++ V DL +R+ +E+ ID +H+IG G NI +I+ + S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+D+FPEV INFP P SD++ L GP+ +V++ +QK A LVE+++S V I ++HK IIG+ G I+++ + +NTK +PA + ++ I+ITGK+ + E A R+ +I + +NI E+ + I LH LIG G I S+ E CGG V I FP + S+TV+I+G DV KA+ +L L EE ++ + E ++FL K++K+R++ R+ +E +D+ + IIG+++ V + L+ + +V +++ + +F + ++ R + + V +S + ++ + +G +++AK I II+D E +T + ++ +++ +++ +I++ + VQI+ P EPV + + + G K + P L ++ + S K + E+N E+ S I A G+A N D L + K E+ ++ L + F L + V+ +++P +IG KG I ++R H V+I+ P+K N ++ +TG ++ AA+ I+ V ELE+M E VP + H ++IG +G+ I KI + V I P S + N +T+ G +NVE+A + +L
Sbjct: 195 KEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVE--KAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR---TEIVFTGEKEQLAQAVARIKKIYEEKANSFTVSSVAAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGG-VHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVK-ELQAEQEDRAL------RSFKLSVTVD--PKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSE----------DVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHIL 1158 HSP 2 Score: 157.147 bits (396), Expect = 6.552e-38 Identity = 97/311 (31.19%), Postives = 173/311 (55.63%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN---INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQ 3826
I+G K+K AKE + L + +EV VP + + +IG KG I+K+ +V+I +P + + +TG + ++ AK ++E V+EL+ +++R +R+F L++ V +YH K+IG KG I++IR ++ V I P +D ++ITI G++ N E A++ +L V +LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N V V G E+V+ D +++ L ++ D L+ Y EE P++ GF +R+APW A ++ S+S++
Sbjct: 920 ISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSG--APDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQ-----VYMKPPAHEEAKAPSR-------GFVVRDAPWTASSSEKAPDMSSSEE 1216 HSP 3 Score: 135.576 bits (340), Expect = 3.050e-31 Identity = 164/712 (23.03%), Postives = 312/712 (43.82%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYS---VRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKS--NELFR--IGRD----NMCKLNVXXXXXXXXXXEQG-ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIPFYPEPVNSKTISNSHVSAKPGEK--PYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN--GSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLE 3667
+++ S V IP ++H+ +IG+ G +++L T TK QIP + +++I ITG ++ +E A V I ++ +E+ K H + G + + + G R+ I P P + ++ G K+ + +A ++ + EE S + F+ L K+ + + +E EDK L G E+V E ++ ++ IN + Y + + ++ R IG+ N K +E+ ++ G Q ++ AK E++ + EN T++LI+++R + I ++ EI K+ +V I +P+P I EK YM + L N + I + + N G NI +I E ++ I P N + ITG + A+ I+ + + ++EV++P + + +LIG KG I+ + V I P + S V++ G S V AKK+++ EE ++ ++F++ + +YH+ LIG G I K+R R+ P ++D + + ITI G +D V +A+ E+ A + L++ + + + +D + H + RG L ++ ++Y V + FP GT+ ++ V +KG K+ C + R +++++ ++ LE
Sbjct: 183 LQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINI--PPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKANSFTVSSVAAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLE--GPTEDVNVAQEQIEGMVKDL-----------INRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIIN---FPDPAQKSDIVQLRGPKNEVEKCTKYMQKM---------VADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDL-------PAENSN--SETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEE-------KQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKD----CVEAAKKRIQEIIEDLEAQVTLE 844 HSP 4 Score: 94.7449 bits (234), Expect = 2.415e-27 Identity = 116/512 (22.66%), Postives = 218/512 (42.58%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDA----PGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQ---IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP---------------------------RDLTK------KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQV-AAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEEN---DEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDA-DSNTVIIKGVKDDVLKAQVKLHDLVEEITA 2053
+ I G V +A LL + + + I E HK++IG G I+++ D T V + + I ++G +D + A + ++++ + D+ P F+I + G L +++ + + F Q + ++G+ + V + +++ + DL+++++ LE I + ++G G IQ+I ++ + I FP +D ++ I G EA K L ++ L +IGQ+G G++++ D F EV I+ P P+ SD+I++ G ++D+ A +L++ E +F V + KYH IIGR+G I ++ + Q P + N D+I ITG +K+ E A + I + ++ + + +H +IG G I + + +V IRFP A D N V + G+ ++V +A + +L EE A
Sbjct: 667 VVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPA--AEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVI-RRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-LECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEAL-----EALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEF-EVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF--KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLA 1166 HSP 5 Score: 49.6766 bits (117), Expect = 2.415e-27 Identity = 39/139 (28.06%), Postives = 69/139 (49.64%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITR----LVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIFLHF 653
V + G K++V + KY+ + LV+ + IS+ I K+FH+++IG G+ K++ ++T++ P N S I I G + E AR I + + I V I + + N L+ RL ++E + +HF
Sbjct: 521 VQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEE--CGGVHIHF 657 HSP 6 Score: 101.679 bits (252), Expect = 6.467e-21 Identity = 54/134 (40.30%), Postives = 79/134 (58.96%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSES---NKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHAR 497
IT F +P EER+Y D + K+ EI G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G ++LE T+T++ P+ +DPS IKI G+K+GIE AR
Sbjct: 112 ITQVFHVPLEERKYKDMNQFGEGEQAKICLEIMQRTGAHLELSLAKDQGLSIMVSGKLDAVMKARKDIVARLQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKAR 245 HSP 7 Score: 77.411 bits (189), Expect = 9.857e-21 Identity = 98/450 (21.78%), Postives = 196/450 (43.56%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNE--------------------------GSP---NTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR----DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDA-DSDLISLIGPRKDVDQ-VAAILQKKHAFLVESNFSKKVKI---PFKYHKAIIGREGITIRELTKATNTKFQIP--AASEE 1744
K + + E + + P +++++ G +D V A + + ++A L+ IP++ H+ ++GP+GS I++I SV + ++ N GSP + I + G K+ A + ++ I ++ P LH+++IG+ GS + V++ V P +Q ++ I I G + L ++V +LQ+ R +L + +DP IIG G I +IR E + +I FP + + +TI G EA + +L+ + +E +D+ H + ++IG +GK +++I D F +V I FP A D + +++ G ++V++ + IL + +L + S+ +++ P + +A G +R+ ++ + P ++SEE
Sbjct: 777 KHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-LECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEAL--EALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQ------SDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF-KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQVYMKPPAHEEAKAPSRGFVVRDAPWTASSSEKAPDMSSSEE 1216 HSP 8 Score: 44.669 bits (104), Expect = 9.857e-21 Identity = 33/118 (27.97%), Postives = 56/118 (47.46%), Query Frame = 3
Query: 174 KLLTEICANCG-VRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQ----FEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPST-LIKIKGSKDGIEHARQLIQ 509
+L+ I CG V + V S V I G V +AKK + L + + F + + E+H+ LIG G +++ ++T +V FP D LI I G +D + A++ ++
Sbjct: 641 RLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELE 758 HSP 9 Score: 81.2629 bits (199), Expect = 1.337e-14 Identity = 113/473 (23.89%), Postives = 210/473 (44.40%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKET-NCSINFPRDLTKKVVTIIGTPHGVE-AVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXX-XXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKL 2026
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++++ +T+ + I R ++ S N I + G K+ + A + L+ + + + ++ H FI G ++G+ N+ SVN+ +IV G E++ + +KK + + + + + + IIG G N+ KI ++ I F K +T+ G V A + I + I +I H LIG+ G + IKD++ +V + PP S+LI + G + V Q L + A +E+ +K + I ++H+ IIG++G IRE+ K P ++++D + + G + +VE K + + K H +IGKGG I ++E ++ + P+ +++S T+II G + + A+ ++
Sbjct: 163 IMVSGKLDAVMKARKDIVARLQTQASAT-----VAIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPDDPS-NQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR-----------TEIVFTGEKEQLAQAVARIKKIYEEKANSFTVSSVAAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDK--ITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQY-KVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELL-ELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDL--PAENSNSETIIITGKRANCEAARSRI 610 HSP 10 Score: 74.3294 bits (181), Expect = 1.531e-12 Identity = 62/286 (21.68%), Postives = 126/286 (44.06%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELV 3616
K+ + G KD + AK+ I ++ + LE +P++F+ +++G KG +I+++ V IK P++ + +I++G E AAK+ + V ++ + VP HR +IG KG I K+ + V I VP+ + + I I G N+++AK +L V +L++ + +D + H ++IG +GA + Q++ ++ V I FP + + + + + G +++ + D ++
Sbjct: 813 KVTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEALEALVP-------------VTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAIL 1085 HSP 11 Score: 68.5514 bits (166), Expect = 9.194e-11 Identity = 58/241 (24.07%), Postives = 112/241 (46.47%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLE--VNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLA-FVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
++G D + A+++I+ V +P+E + +IG GEK++ L I+IP + ++ +TG+ E + A+ E++ E ++ ER L V +H + G + +I + RI++P N EI G K+ + +A + + K S + + S +H +IG +G NL ++ ++ KV I F TEGE+ + ++G E V+ +++
Sbjct: 165 VSGKLDAVMKARKDIVARLQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVER--------LEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKANSFTVSSVAAPSWLHRFIIGKKGQNLAKITQQMPKVHIEF----TEGEDK--ITLEGPTEDVNVAQEQI 391 HSP 12 Score: 62.003 bits (149), Expect = 8.852e-9 Identity = 34/124 (27.42%), Postives = 65/124 (52.42%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIG 3469
++V+G ++V+ A+K+IV ++ S + +P ++HR +IG GE + + K + +I +P D N+I I G K+ +EKA++EVL + + +E L ++ H + G
Sbjct: 163 IMVSGKLDAVMKARKDIVARLQT-----------QASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAG 275
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Human
Match: HDLBP (high density lipoprotein binding protein [Source:HGNC Symbol;Acc:HGNC:4857]) HSP 1 Score: 289.656 bits (740), Expect = 6.027e-81 Identity = 205/692 (29.62%), Postives = 369/692 (53.32%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAA-SILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFT----KSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQ-KSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
K +H+ K+ L + + + ++ IP N+I I+G ++G+ +A +LL + Q K + L++E K H +I GP + EI+ +T ++I +N I G K+ L A+ + K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V E ++ V DL +R+ +E+ ID +H+IG G NI +I+ + S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+D+FPEV INFP P SD++ L GP+ +V++ +QK A LVE+++S V I ++HK IIG+ G I+++ + +NTK +PA + ++ I+ITGK+ + E A R+ +I + +NI E+ + I LH LIG G I S+ E CGG V I FP + S+TV+I+G DV KA+ +L L EE ++ + E ++FL K++K+R++ R+ +E +D+ + IIG+++ V + L+ + +V +++ + +F + ++ R + + V +S + ++ + +G +++AK I II+D E +T + ++ +++ +++ +I++ + VQI+ P
Sbjct: 37 KEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVE--KAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR---TEIVFTGEKEQLAQAVARIKKIYEEKANSFTVSSVAAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGG-VHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFP 719 HSP 2 Score: 134.42 bits (337), Expect = 4.593e-31 Identity = 164/712 (23.03%), Postives = 312/712 (43.82%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYS---VRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKS--NELFR--IGRD----NMCKLNVXXXXXXXXXXEQG-ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIPFYPEPVNSKTISNSHVSAKPGEK--PYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN--GSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLE 3667
+++ S V IP ++H+ +IG+ G +++L T TK QIP + +++I ITG ++ +E A V I ++ +E+ K H + G + + + G R+ I P P + ++ G K+ + +A ++ + EE S + F+ L K+ + + +E EDK L G E+V E ++ ++ IN + Y + + ++ R IG+ N K +E+ ++ G Q ++ AK E++ + EN T++LI+++R + I ++ EI K+ +V I +P+P I EK YM + L N + I + + N G NI +I E ++ I P N + ITG + A+ I+ + + ++EV++P + + +LIG KG I+ + V I P + S V++ G S V AKK+++ EE ++ ++F++ + +YH+ LIG G I K+R R+ P ++D + + ITI G +D V +A+ E+ A + L++ + + + +D + H + RG L ++ ++Y V + FP GT+ ++ V +KG K+ C + R +++++ ++ LE
Sbjct: 25 LQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINI--PPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKANSFTVSSVAAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLE--GPTEDVNVAQEQIEGMVKDL-----------INRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIIN---FPDPAQKSDIVQLRGPKNEVEKCTKYMQKM---------VADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDL-------PAENSN--SETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEE-------KQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKD----CVEAAKKRIQEIIEDLEAQVTLE 686 HSP 3 Score: 100.138 bits (248), Expect = 1.623e-20 Identity = 124/557 (22.26%), Postives = 247/557 (44.34%), Query Frame = 2
Query: 896 TICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQL-RFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIG----TPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK-SNELF----RIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDER 2521
+I + G D + A + + ++ + P H+F+IGK+G L DL + IQ+ R +D +NQI I G+ +E IE + + + Q + + +E++ I G + +I +ET IN P + + + G V +K I + + + + H +IG++G+ L +I + P+V I F D I+L GP +DV+ ++ L+ ++ I K+H+ +IG+ G I + +IP SE+++ I I G + V+ A + + + + N + I + H +IG+ G RI +++ V I FP P S+ V ++G K++V K + +V ++ ++Y + + ++ + +KK+RE + +I +E + + I G++ N + ARS + I + ++ +++ K N L R+ R M + + + + RGP +++ AK +++H+ ++ + T++ VD R
Sbjct: 4 SIMVSGKLDAVMKARKDIVARLQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGT-KEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKANSFTVSSVAAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKF-PEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANC-------EAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQ---TKSFTVDIR 542 HSP 4 Score: 65.4698 bits (158), Expect = 1.440e-18 Identity = 83/389 (21.34%), Postives = 162/389 (41.65%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDA----PGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQ---IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP---------------------------RDLTK------KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQV-AAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIREL 1696
+ I G V +A LL + + + I E HK++IG G I+++ D T V + + I ++G +D + A + ++++ + D+ P F+I + G L +++ + + F Q + ++G+ + V + +++ + DL+++++ LE I + ++G G IQ+I ++ + I FP +D ++ I G EA K L ++ L +IGQ+G G++++ D F EV I+ P P+ SD+I++ G ++D+ A +L++ E +F V + KYH IIGR+G I ++
Sbjct: 509 VVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPA--AEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVI-RRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-LECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEAL-----EALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEF-EVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQI 887 HSP 5 Score: 49.6766 bits (117), Expect = 1.440e-18 Identity = 39/139 (28.06%), Postives = 69/139 (49.64%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITR----LVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIFLHF 653
V + G K++V + KY+ + LV+ + IS+ I K+FH+++IG G+ K++ ++T++ P N S I I G + E AR I + + I V I + + N L+ RL ++E + +HF
Sbjct: 363 VQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEE--CGGVHIHF 499 HSP 6 Score: 83.5741 bits (205), Expect = 2.066e-15 Identity = 46/134 (34.33%), Postives = 82/134 (61.19%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRI 3304
I+G K+K AKE + L + +EV VP + + +IG KG I+K+ +V+I +P + + +TG + ++ AK ++E V+EL+ +++R +R+F L++ V +YH K+IG KG I++IR ++ V I
Sbjct: 762 ISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNI 895 HSP 7 Score: 81.2629 bits (199), Expect = 1.092e-14 Identity = 113/473 (23.89%), Postives = 210/473 (44.40%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKET-NCSINFPRDLTKKVVTIIGTPHGVE-AVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXX-XXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKL 2026
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++++ +T+ + I R ++ S N I + G K+ + A + L+ + + + ++ H FI G ++G+ N+ SVN+ +IV G E++ + +KK + + + + + + IIG G N+ KI ++ I F K +T+ G V A + I + I +I H LIG+ G + IKD++ +V + PP S+LI + G + V Q L + A +E+ +K + I ++H+ IIG++G IRE+ K P ++++D + + G + +VE K + + K H +IGKGG I ++E ++ + P+ +++S T+II G + + A+ ++
Sbjct: 5 IMVSGKLDAVMKARKDIVARLQTQASAT-----VAIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPDDPS-NQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR-----------TEIVFTGEKEQLAQAVARIKKIYEEKANSFTVSSVAAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDK--ITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQY-KVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELL-ELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDL--PAENSNSETIIITGKRANCEAARSRI 452 HSP 8 Score: 77.7962 bits (190), Expect = 1.336e-13 Identity = 38/86 (44.19%), Postives = 56/86 (65.12%), Query Frame = 3
Query: 240 SLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHAR 497
L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G ++LE T+T++ P+ +DPS IKI G+K+GIE AR
Sbjct: 2 GLSIMVSGKLDAVMKARKDIVARLQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKAR 87 HSP 9 Score: 49.2914 bits (116), Expect = 1.890e-12 Identity = 60/286 (20.98%), Postives = 115/286 (40.21%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNE--------------------------GSP---NTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSI 1285
K + + E + + P +++++ G +D V A + + ++A L+ IP++ H+ ++GP+GS I++I SV + ++ N GSP + I + G K+ A + ++ I ++ P LH+++IG+ GS + V++ V P +Q ++ I I G + L ++V +LQ+ R +L + +DP IIG G I +IR E + +I
Sbjct: 619 KHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-LECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEAL--EALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQ------SDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNI 895 HSP 10 Score: 45.0542 bits (105), Expect = 1.890e-12 Identity = 33/118 (27.97%), Postives = 56/118 (47.46%), Query Frame = 3
Query: 174 KLLTEICANCG-VRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQ----FEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPST-LIKIKGSKDGIEHARQLIQ 509
+L+ I CG V + V S V I G V +AKK + L + + F + + E+H+ LIG G +++ ++T +V FP D LI I G +D + A++ ++
Sbjct: 483 RLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELE 600 HSP 11 Score: 67.781 bits (164), Expect = 1.271e-10 Identity = 58/255 (22.75%), Postives = 112/255 (43.92%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIV 3523
K+ + G KD + AK+ I ++ + LE +P++F+ +++G KG +I+++ V IK P++ + +I++G E AAK+ + V ++ + VP HR +IG KG I K+ + V I VP+ + + I I G N+++AK +L V +L++ + +D + H ++IG +GA + Q++ ++ V I+
Sbjct: 655 KVTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEALEALVP-------------VTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIL 896 HSP 12 Score: 67.781 bits (164), Expect = 1.571e-10 Identity = 58/241 (24.07%), Postives = 112/241 (46.47%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLE--VNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLA-FVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
++G D + A+++I+ V +P+E + +IG GEK++ L I+IP + ++ +TG+ E + A+ E++ E ++ ER L V +H + G + +I + RI++P N EI G K+ + +A + + K S + + S +H +IG +G NL ++ ++ KV I F TEGE+ + ++G E V+ +++
Sbjct: 7 VSGKLDAVMKARKDIVARLQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVER--------LEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKANSFTVSSVAAPSWLHRFIIGKKGQNLAKITQQMPKVHIEF----TEGEDK--ITLEGPTEDVNVAQEQI 233 HSP 13 Score: 61.6178 bits (148), Expect = 1.081e-8 Identity = 34/124 (27.42%), Postives = 65/124 (52.42%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIG 3469
++V+G ++V+ A+K+IV ++ S + +P ++HR +IG GE + + K + +I +P D N+I I G K+ +EKA++EVL + + +E L ++ H + G
Sbjct: 5 IMVSGKLDAVMKARKDIVARLQT-----------QASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAG 117
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Celegans
Match: vgln-1 (ViGiLN homolog [Source:UniProtKB/TrEMBL;Acc:B3GWB2]) HSP 1 Score: 271.937 bits (694), Expect = 6.615e-88 Identity = 210/681 (30.84%), Postives = 362/681 (53.16%), Query Frame = 2
Query: 593 NTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI-----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSIN-DLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGI----LEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
N + + ++++IPP V IS++G++DGV + A+ + Q + K +Q+ + + +H+YI+G SGI ++L +T V++ + + GS + + L+G +L AL LV ++ SV +I AP WLHK +IG G+ L L N+ N+Q+ F D +NQI +EGSPEEV E L K+V LQ L+ ++++ P + +H+IG GG I KI+ + I P + T + + G GV+ + + IEN K +DII L +IG +G G++ I+D P V + FP + SD++++ G + +VD V L E+N+ + V I ++ K I+G+ G +IR+L T T+ +P + ++ +I +TGKQ +VE A+ ++ I E+ +N+ E IEI + + + G G I+ +++ CGG V IRFPS ++S V I+G DV KA L L ++ + E + E + FL K+ KLR+T +VR+ +AE K + ++G+KE+V L+ A ++ +ET+ + I D Y++ N L R G + ++ N + + G + RG Q +++AK+ I +++DYE IT+N+ + + + + ++ E+ KY V I+ P
Sbjct: 227 NETYDRLTQNNGVKINIPPPHVTNEVISVTGEKDGVLRVAAEIRQII-ESKKNVSSIQVAVARTQHRYIVGQSRSGIHDVLQKTGCVVEVPAE--DSGS-DQVTLIGNAQDLAKALALVIERASSVVTQSISAPNWLHKHLIGPKGATLTALVPNRNNVQIEF-DNSNQIFLEGSPEEVKLAFEPLSKEVARLQMELAIEKVKVHPTLHRHVIGRGGSLISKIKDQHGVQITIPNEETNSDEIVVEGKKEGVKKAVTEIRAIVTKIENEKSRDIIIPQRLHKLIIGSKGSGVQVIRDSHPNVSVVFPDAKSKSDVVNIRGDKTEVDAVYKKLTALSKEYAENNYQQTVAIFKEFLKHIVGKGGASIRKLRDETETRIDLPESGSDDGKITVTGKQANVEKAVAQLNKIQEELANVAEESIEIPQKVQSRFFGNGRRLISDIEDECGG-VHIRFPSEKSESTKVTIRGPAGDVAKAVGLLSALAKDKEENYVEDTVKAKPEFHRFLIGKGGSKIAKLRDTLNVRVMFPKEGDAE-KETIHLLGKKEDVPKAKAALEDAIKQL----SETVDIKITVDPKYYK--NFLAR-GAALVKEIQEQNGGVVISFPKNGTDSSEVSIRGSKQCVEAAKNRIEDVVEDYEKQITDNVTIPAQFHRGLLAGRGAKIHELQSKYNVSIRFP 893 HSP 2 Score: 73.9442 bits (180), Expect = 6.615e-88 Identity = 43/124 (34.68%), Postives = 65/124 (52.42%), Query Frame = 3
Query: 150 DSESNKLK---------KLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHA 494
D SNK+K K I R+E + + D L V + G++ K EA+ I R +QTQ +DIPK+ H LIG +G+L + LE T+ ++ P + PS+ I I G ++GI+ A
Sbjct: 70 DERSNKVKSFGSTSEESKKAQAIATATKTRIELSESKDGELTVVVKGERAKAEEARARIIRDLQTQASREIDIPKDHHGRLIGKEGALLRNLEAETNCRIQIPNRDGPSSKITITGPREGIQRA 193 HSP 3 Score: 145.591 bits (366), Expect = 9.661e-35 Identity = 91/299 (30.43%), Postives = 164/299 (54.85%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKES-VDRCADEL-VDRANIMLD--EINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPW 3778
++ ++G K+ AKE ++ + + + +P + + ++IG GE ++KL + V+I IP NS + VTG +E+V A + + + E E ++R+++ +S++++VP YH+K+IG +G TI+ ++ K V I+VP +D N ITIQG+++ + + +++L S + +++D+R H RLIG RG NL ++ + Y+VEI P +G E+ N+V V G E+ V C D L + +LD E M + +S+N + QP + Q++ APW
Sbjct: 905 QVTVSGRDTKVEEAKEALLAMVPISKVIQLPVDMHRSIIGRGGETVRKLMQDYDVNISIPKDNSS-EDITVTGQTENVDQALEALRGKLGEYEAQAEDRKLKQWSMSINVPTDYHQKIIGQRGATITALKEKYGVIINVPREDGN-ETITIQGYEEKANECAAAIEEMISELRSMFTQEISLDARYHPRLIGQRGKNLKKVMEDYRVEIRLPRQG--AEDPNLVVVAGKDENDVYDCIDHLRAEEEEFLLDNVERTQYMSPRQQESNNTRHHQQP--------------TSVQMKGAPW 1185 HSP 4 Score: 128.257 bits (321), Expect = 1.769e-29 Identity = 203/877 (23.15%), Postives = 380/877 (43.33%), Query Frame = 2
Query: 1100 IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP-RDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLK-EIKDRFPE---VRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESN---FSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRIS---EAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELF--------RIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDY-ENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITL-------AKE-EIIKLFVLDLEVNVPE-------EFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVI-VTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNS-VRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEE-ANIVKVKGTKESVDRCADELV 3616
+V++G E + E + + DLQ++ S+ E++I +IG G ++ + ETNC I P RD +TI G G++ A +L E + H + P+ + +G K E DR + V+IN PPP +++IS+ G + V +VAA +++ ++ES S +V + H+ I+G+ I ++ + T ++PA +D++ + G +D+ A+ V + S+++ I LH LIG G + +L + V I F +SN + ++G ++V A L V + + ++ + + + K+++ + V+I+ E + + + G+KE V +KA +E+ IVT+ D+ Q+ ++L ++ RD+ ++V +KS + ++ RG + + ++ + +Y EN + + + + LK+I + ++ + + +I +P T++ + + V +E +++ +P + S NG + IS+I E GV +I+ P K E T K+T+ AK ++ D E N E EF+ LIG G KI KLR T V + P + + I + G E V AK + + +++L + + V +Y++ + + +I+ +N V IS P ++ +E++I+G K VE AKN + V E I + + I ++ H L+ RGA + +L+ KY V I FP EG E ++ V V G V+ + L+
Sbjct: 112 VVVKG---ERAKAEEARARIIRDLQTQASR-EIDIPKDHHGRLIGKEGALLRNLEAETNCRIQIPNRDGPSSKITITGPREGIQRAAAHILAVSEREAKLATE-----HIVCPKNLVAFVRGPKNETYDRLTQNNGVKINIPPPHVTNEVISVTGEKDGVLRVAAEIRQ----IIESKKNVSSIQVAVARTQHRYIVGQSRSGIHDVLQKTGCVVEVPAEDSGSDQVTLIGNAQDLAKALALVIE---RASSVVTQSISAPNWLHKHLIGPKGATLTALVPNRNN-VQIEFD----NSNQIFLEGSPEEVKLAFEPLSKEVARLQMELAIEKVKVHPTLHRHVIGRGGSLISKIKDQHGVQITIPNEETNSDEIVVEGKKEGV-------KKAVTEIRAIVTKIENEKSRDIIIPQRLHKLIIGSKGSGVQVIRDSHPNVSVVFPDAKS---KSDVVNIRGDKTEVDAVYKKLTALSKEYAENNYQQTVAIFKEFLKHIVGKGGASIRKLRDETETRIDLPESGSDDGKITVTGKQANVE-------KAVAQLNKIQEELANVAEESIEIPQKVQSRFFGNGRRL--ISDIEDEC-----GGV--------HIRFPSEKSEST----KVTIRGPAGDVAKAVGLLSALAKDKEENYVEDTVKAKPEFHRFLIGKGGSKIAKLRDTLNVRVMFPKEGDAEKETIHLLGKKEDVPKAKAALEDAIKQLSE--------TVDIKITVDPKYYKNFLARGAALVKEIQEQNGGVVISFPKNGTDSSEVSIRGSKQCVEAAKNRIEDVVEDYEKQITDNVTIPAQFHRGLLAGRGAKIHELQSKYNVSIRFPNNREEGSEGSDQVTVSGRDTKVEEAKEALL 923 HSP 5 Score: 112.849 bits (281), Expect = 6.312e-28 Identity = 134/569 (23.55%), Postives = 257/569 (45.17%), Query Frame = 2
Query: 587 KSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNT-ICLLGPKDNLGPALTLV--FTKSESVNII--AIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQ----IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETN-CSINFPRDLTKKV-VTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFP----PPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXL--------HTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKEN 2212
K ++ + + R+D+P D +I+++G + V +A + L + ++ A E IEIP++ G I +I D+ ++ + +E S +T + + GP ++ A+ L+ K + N + + A H+F+IGK GS + L + N+++ F E + I + G E+V + L+ + L S +++ +DP K+ + G +++I+++ I+FP++ T V+I G+ VEA K + +E E ++ L+ +G + E++ ++ V I FP SD +++ G V++ K A L SK +++P H++IIGR G T+R+L + + IP + D I +TG+ ++V+ A++ + + E ++ H +IG+ G I +LKE G V I P D + T+ I+G ++ + + +++ E+ S + Q + + L LKK+ E Y V I AED + + + G+ EN
Sbjct: 582 KGGASIRKLRDETETRIDLPESGSDDGKITVTGKQANVEKAVAQLNKIQEEL-ANVAEESIEIPQKVQSRFFGNGRRLISDIEDECG---GVHIRFPSEKSESTKVTIRGPAGDVAKAVGLLSALAKDKEENYVEDTVKAKPEFHRFLIGKGGSKIAKLR-DTLNVRVMFPKEGDAEKETIHLLGKKEDVPKAKAALEDAIKQL-SETVDIKITVDPKYYKNFLARGAALVKEIQEQNGGVVISFPKNGTDSSEVSIRGSKQCVEAAKNRIEDVVEDYEKQITDNVTIPAQFHRGLLAGRGAKIHELQSKY-NVSIRFPNNREEGSEGSDQVTVSGRDTKVEEA------KEALLAMVPISKVIQLPVDMHRSIIGRGGETVRKLMQDYDVNISIPKDNSSED-ITVTGQTENVDQALEALRGKLGEYEAQAEDRKLKQWSMSINVPTDYHQKIIGQRGATITALKEKYG--VIINVPREDGN-ETITIQGYEEKANECAAAIEEMISELR-SMFTQEISLDARYHPRLIGQRGKNLKKVMEDYRVEIRLPRQGAEDPNLVVVAGKDEN 1132 HSP 6 Score: 32.7278 bits (73), Expect = 6.312e-28 Identity = 29/104 (27.88%), Postives = 55/104 (52.88%), Query Frame = 3
Query: 177 LLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLV-QTQFEISLDI--PKEFHRSLIGNKGSLHKEL-ENNTSTQVSFPKINDPSTLIKIKGSK 476
L+++I GV++ S + + G+K+ V +A I +V + + E S DI P+ H+ +IG+KGS + + +++ + V FP S ++ I+G K
Sbjct: 440 LISKIKDQHGVQITIPNEETNSDEIVVEGKKEGVKKAVTEIRAIVTKIENEKSRDIIIPQRLHKLIIGSKGSGVQVIRDSHPNVSVVFPDAKSKSDVVNIRGDK 543 HSP 7 Score: 116.316 bits (290), Expect = 7.401e-26 Identity = 125/555 (22.52%), Postives = 252/555 (45.41%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAID---APGWLHKFIIGK--------SGSNLVDLSVNKPNIQLRFEDEANQIV-IEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP-RDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAE---DKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLAL 2287
+ + G+R +A + +++ T +I+IPK+ H +IG G+ ++ + +T+ + I + +G + I + GP++ + A + SE +A + P L F+ G + +N V +++ P++ N+++ + G + V+ V+ + +++ + + +S +++ + ++I+G I + ++T C + P D VT+IG + A++++ ++ + H LIG +G L + V+I F +S+ I L G ++V L K+ A L +KVK+ H+ +IGR G I ++ + IP +DEIV+ GK++ V+ A+ + I K N I I + LH ++IG G+ + +++S V++ FP + S+ V I+G K +V KL L +E +NY+Q + E + + ++KLR+ RI E D + + G++ NV + L K + E+ + E++ +
Sbjct: 112 VVVKGERAKAEEARARIIRDLQ----TQASREIDIPKDHHGRLIGKEGALLRNLEAETNCRIQIPNR---DGPSSKITITGPREGIQRAAAHILAVSEREAKLATEHIVCPKNLVAFVRGPKNETYDRLTQNNGVKINIPPPHV-------TNEVISVTGEKDGVLRVAAEI-RQIIESKKNVSSIQVAVARTQHRYIVGQSRSGIHDVLQKTGCVVEVPAEDSGSDQVTLIGNAQDLAKALALVIERASSVVTQSISAPNWLHK---HLIGPKGATLTALVPNRNNVQIEFD----NSNQIFLEGSPEEVKLAFEPLSKEVARLQMELAIEKVKVHPTLHRHVIGRGGSLISKIKDQHGVQITIPNEETNSDEIVVEGKKEGVKKAVTEIRAIVTKIENEKSRDIIIPQRLHKLIIGSKGSGVQVIRDSHPN-VSVVFPDAKSKSDVVNIRGDKTEVDAVYKKLTALSKEYAENNYQQTVAIFKEFLKHIVGKGGASIRKLRDETETRIDLPESGSDDGKITVTGKQANVEKAVAQLNKIQEELANVAEESIEI 643 HSP 8 Score: 80.4925 bits (197), Expect = 8.006e-15 Identity = 116/508 (22.83%), Postives = 223/508 (43.90%), Query Frame = 2
Query: 629 LRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTK----SESVNI-IAIDAPGWLHKFIIGKSGSNLV-DLSVNKPNIQLRFED---EANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKV-----VTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHA-FLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDA-DSNTVIIKGV-KDDVLKAQVKLHDLVEEITASNYEQHFQMT 2083
+ + P + +++I G VA+A +L + Y E ++ E H+++IG GS I ++ D +V V ++ + TI LLG K+++ A + SE+V+I I +D P + F+ G+ LV ++ + + F +++++ I GS + V ++ V D + +++ + I + ++ G I +++ + N SI FP + + VT+ G VE K LL + + + + +IG+ G+ ++++ + +V I+ P D S+ I++ G ++VDQ L+ K + ++ +S + +P YH+ IIG+ G TI L + +P + N+ I I G ++ + + + + ++ I + H LIG+ G + + E RV IR P A D N V++ G ++DV L EE N E+ M+
Sbjct: 669 VHIRFPSEKSESTKVTIRGPAGDVAKAVGLLSALAKDKEENYVEDTVKAKPEFHRFLIGKGGSKIAKLRDTLNVRVMFPKE--GDAEKETIHLLGKKEDVPKAKAALEDAIKQLSETVDIKITVD-PKYYKNFL--ARGAALVKEIQEQNGGVVISFPKNGTDSSEVSIRGSKQCVEAAKNRIEDVVEDYEKQITD-NVTIPAQFHRGLLAGRGAKIHELQSKYNVSIRFPNNREEGSEGSDQVTVSGRDTKVEEAKEALLAMVPISKVIQ-----LPVDMHRSIIGRGGETVRKLMQDY-DVNISIPK-DNSSEDITVTGQTENVDQALEALRGKLGEYEAQAEDRKLKQWSMSINVPTDYHQKIIGQRGATITALKEKYGVIINVP-REDGNETITIQGYEEKANECAAAIEEMISELRSMFTQEISLDARYHPRLIGQRGKNLKKVMEDY--RVEIRLPRQGAEDPNLVVVAGKDENDVYDCIDHLRAEEEEFLLDNVERTQYMS 1160
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Celegans
Match: vgln-1 (ViGiLN homolog [Source:UniProtKB/TrEMBL;Acc:B3GWB2]) HSP 1 Score: 178.333 bits (451), Expect = 7.854e-60 Identity = 153/550 (27.82%), Postives = 267/550 (48.55%), Query Frame = 2
Query: 593 NTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIP----AASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRIIGQKENVLNTL 2227
N + + ++++IPP V IS++G++DGV + A+ + Q + K +Q+ + + +H+YI+G SGI ++L +T V++ + + GS + + L+G +L AL LV ++ SV +I AP WLHK +IG G+ L L N+ N+Q+ F D +NQI +EGSPEEV E L K+V LQ L+ ++++ P + +H+IG GG I KI+ + I P + T + + G GV+ KA L I+ + T I + + +KEI+++ V I+FP DS +S+ G ++ V+ ++ E + V IP ++H+ ++ G I EL N + P SE +D++ ++G+ VE+A + + + I I++ ++H +IG+GG + L + V I P D S + + G ++V +A + + L +YE + D KLK+ +S+ I+ D H +IIGQ+ + L
Sbjct: 227 NETYDRLTQNNGVKINIPPPHVTNEVISVTGEKDGVLRVAAEIRQII-ESKKNVSSIQVAVARTQHRYIVGQSRSGIHDVLQKTGCVVEVPAE--DSGS-DQVTLIGNAQDLAKALALVIERASSVVTQSISAPNWLHKHLIGPKGATLTALVPNRNNVQIEF-DNSNQIFLEGSPEEVKLAFEPLSKEVARLQMELAIEKVKVHPTLHRHVIGRGGSLISKIKDQHGVQITIPNEETNSDEIVVEGKKEGVK--KAALEDAIKQLSETVDIKITVDPKYYKNFLARGAALVKEIQEQNGGVVISFPKNGTDSSEVSIRGSKQCVEAAKNRIEDV-VEDYEKQITDNVTIPAQFHRGLLAGRGAKIHELQSKYNVSIRFPNNREEGSEGSDQVTVSGRDTKVEEAKEALLAMV-----PISKVIQLPVDMHRSIIGRGGETVRKLMQDYD--VNISIPK-DNSSEDITVTGQTENVDQAL----------------EALRGKLGEYE--AQAEDRKLKQ----WSMSINVPTD-YHQKIIGQRGATITAL 737 HSP 2 Score: 73.9442 bits (180), Expect = 7.854e-60 Identity = 43/124 (34.68%), Postives = 65/124 (52.42%), Query Frame = 3
Query: 150 DSESNKLK---------KLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHA 494
D SNK+K K I R+E + + D L V + G++ K EA+ I R +QTQ +DIPK+ H LIG +G+L + LE T+ ++ P + PS+ I I G ++GI+ A
Sbjct: 70 DERSNKVKSFGSTSEESKKAQAIATATKTRIELSESKDGELTVVVKGERAKAEEARARIIRDLQTQASREIDIPKDHHGRLIGKEGALLRNLEAETNCRIQIPNRDGPSSKITITGPREGIQRA 193 HSP 3 Score: 146.362 bits (368), Expect = 3.538e-35 Identity = 91/299 (30.43%), Postives = 164/299 (54.85%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKES-VDRCADEL-VDRANIMLD--EINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPW 3778
++ ++G K+ AKE ++ + + + +P + + ++IG GE ++KL + V+I IP NS + VTG +E+V A + + + E E ++R+++ +S++++VP YH+K+IG +G TI+ ++ K V I+VP +D N ITIQG+++ + + +++L S + +++D+R H RLIG RG NL ++ + Y+VEI P +G E+ N+V V G E+ V C D L + +LD E M + +S+N + QP + Q++ APW
Sbjct: 610 QVTVSGRDTKVEEAKEALLAMVPISKVIQLPVDMHRSIIGRGGETVRKLMQDYDVNISIPKDNSS-EDITVTGQTENVDQALEALRGKLGEYEAQAEDRKLKQWSMSINVPTDYHQKIIGQRGATITALKEKYGVIINVPREDGN-ETITIQGYEEKANECAAAIEEMISELRSMFTQEISLDARYHPRLIGQRGKNLKKVMEDYRVEIRLPRQG--AEDPNLVVVAGKDENDVYDCIDHLRAEEEEFLLDNVERTQYMSPRQQESNNTRHHQQP--------------TSVQMKGAPW 890 HSP 4 Score: 96.6709 bits (239), Expect = 6.607e-20 Identity = 116/519 (22.35%), Postives = 231/519 (44.51%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAID---APGWLHKFIIGK--------SGSNLVDLSVNKPNIQLRFEDEANQIV-IEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP-RDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDV-----EDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHF----QMTLEDYEFLFMSADLKLKKLRETYSVRI 2161
+ + G+R +A + +++ T +I+IPK+ H +IG G+ ++ + +T+ + I + +G + I + GP++ + A + SE +A + P L F+ G + +N V +++ P++ N+++ + G + V+ V+ + +++ + + +S +++ + ++I+G I + ++T C + P D VT+IG + A++++ ++ + H LIG +G L + V+I F +S+ I L G ++V L K+ A L +KVK+ H+ +IGR G I ++ + IP +DEIV+ GK++ V EDAIK++ +I I + + + +G + ++E GG V I FP DS+ V I+G K V A+ ++ D+VE +YE+ + + + L K+ +L+ Y+V I
Sbjct: 112 VVVKGERAKAEEARARIIRDLQ----TQASREIDIPKDHHGRLIGKEGALLRNLEAETNCRIQIPNR---DGPSSKITITGPREGIQRAAAHILAVSEREAKLATEHIVCPKNLVAFVRGPKNETYDRLTQNNGVKINIPPPHV-------TNEVISVTGEKDGVLRVAAEI-RQIIESKKNVSSIQVAVARTQHRYIVGQSRSGIHDVLQKTGCVVEVPAEDSGSDQVTLIGNAQDLAKALALVIERASSVVTQSISAPNWLHK---HLIGPKGATLTALVPNRNNVQIEFD----NSNQIFLEGSPEEVKLAFEPLSKEVARLQMELAIEKVKVHPTLHRHVIGRGGSLISKIKDQHGVQITIPNEETNSDEIVVEGKKEGVKKAALEDAIKQLSETV-------DIKITVDPKYYKNFLARGAALVKEIQEQNGG-VVISFPKNGTDSSEVSIRGSKQCVEAAKNRIEDVVE-----DYEKQITDNVTIPAQFHRGLLAGRGAKIHELQSKYNVSI 595 HSP 5 Score: 86.2705 bits (212), Expect = 1.135e-16 Identity = 122/517 (23.60%), Postives = 237/517 (45.84%), Query Frame = 2
Query: 1100 IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP-RDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLK-EIKDRFPE---VRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESN---FSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRIS---EAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSIN-DLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGI----LEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
+V++G E + E + + DLQ++ S+ E++I +IG G ++ + ETNC I P RD +TI G G++ A +L E + H + P+ + +G K E DR + V+IN PPP +++IS+ G + V +VAA +++ ++ES S +V + H+ I+G+ I ++ + T ++PA +D++ + G +D+ A+ V + S+++ I LH LIG G + +L + V I F +SN + ++G ++V A L V + + ++ + + + K+++ + V+I+ E + + + G+KE V L+ A ++ +ET+ + I D Y++ N L R G + ++ N + + G + RG Q +++AK+ I +++DYE IT+N+ + + + + ++ E+ KY V I+ P
Sbjct: 112 VVVKG---ERAKAEEARARIIRDLQTQASR-EIDIPKDHHGRLIGKEGALLRNLEAETNCRIQIPNRDGPSSKITITGPREGIQRAAAHILAVSEREAKLATE-----HIVCPKNLVAFVRGPKNETYDRLTQNNGVKINIPPPHVTNEVISVTGEKDGVLRVAAEIRQ----IIESKKNVSSIQVAVARTQHRYIVGQSRSGIHDVLQKTGCVVEVPAEDSGSDQVTLIGNAQDLAKALALVIE---RASSVVTQSISAPNWLHKHLIGPKGATLTALVPNRNN-VQIEFD----NSNQIFLEGSPEEVKLAFEPLSKEVARLQMELAIEKVKVHPTLHRHVIGRGGSLISKIKDQHGVQITIPNEETNSDEIVVEGKKEGVKKA--ALEDAIKQL----SETVDIKITVDPKYYK--NFLAR-GAALVKEIQEQNGGVVISFPKNGTDSSEVSIRGSKQCVEAAKNRIEDVVEDYEKQITDNVTIPAQFHRGLLAGRGAKIHELQSKYNVSIRFP 598 HSP 6 Score: 84.3445 bits (207), Expect = 4.033e-16 Identity = 70/246 (28.46%), Postives = 121/246 (49.19%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIK-LFVLDLEVNVPE-EFYPAL----IGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNS-VRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEE-ANIVKVKGTKESVDRCADELV 3616
+ G +++ LA E + K + L +E+ + + + +P L IG G I K++ H V I IPN+ + +++V G E V A E + +KQ E + + V +Y++ + + +I+ +N V IS P ++ +E++I+G K VE AKN + V E I + + I ++ H L+ RGA + +L+ KY V I FP EG E ++ V V G V+ + L+
Sbjct: 393 LEGSPEEVKLAFEPLSKEVARLQMELAIEKVKVHPTLHRHVIGRGGSLISKIKDQHGVQITIPNEETNSDEIVVEGKKEGVKKAALE--------DAIKQLSE--TVDIKITVDPKYYKNFLARGAALVKEIQEQNGGVVISFPKNGTDSSEVSIRGSKQCVEAAKNRIEDVVEDYEKQITDNVTIPAQFHRGLLAGRGAKIHELQSKYNVSIRFPNNREEGSEGSDQVTVSGRDTKVEEAKEALL 628 HSP 7 Score: 75.485 bits (184), Expect = 2.014e-13 Identity = 94/431 (21.81%), Postives = 211/431 (48.96%), Query Frame = 2
Query: 569 HQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTI-----CLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRF-------EDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKVVTIIGTPHGV----EAVKAILLXXXXXXXXXXXXXXXFTHTL----MPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIP-AASEENDEIVITGK-QKDVEDAI 1795
H+ + + + + ++ +++ IP + +EI + G ++GV +AA L Q+ T +++I + + +K + + +KEI +Q V + + S +I C+ K+ + + + K + N+ P H+ ++ G+ + +L +K N+ +RF + ++Q+ + G +V E E L V S++ QL ++ M + IIG GG ++K+ ++ + +I+ P+D + + +T+ G V EA++ L + E+ K K + + ++IGQ+G + +K+++ V IN P D + + I++ G + ++ AA +++ + L S F++++ + +YH +IG+ G ++++ + + ++P +E+ + +V+ GK + DV D I
Sbjct: 430 HRHVIGRGGSLISKIKDQHGVQITIPNEETNSDEIVVEGKKEGVKKAA--LEDAIKQLSETV-DIKITVDPKYYKNFLARGAALVKEIQEQNGGVVISFPKNGTDSSEVSIRGSKQCVEAAKNRIEDVVE-DYEKQITDNVTI---PAQFHRGLLAGRGAKIHELQ-SKYNVSIRFPNNREEGSEGSDQVTVSGRDTKVEEAKEALLAMV--PISKVIQLPVD----MHRSIIGRGGETVRKLMQDYDVNISIPKDNSSEDITVTGQTENVDQALEALRGKLGEYEAQAEDRKLKQWSMSINVPTDYHQKIIGQRGATITALKEKYG-VIINVPREDGN-ETITIQGYEEKANECAAAIEEMISEL-RSMFTQEISLDARYHPRLIGQRGKNLKKVMEDYRVEIRLPRQGAEDPNLVVVAGKDENDVYDCI 843 HSP 8 Score: 60.077 bits (144), Expect = 1.303e-8 Identity = 53/216 (24.54%), Postives = 97/216 (44.91%), Query Frame = 2
Query: 2867 ISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVN-----VPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGII-EVLNIDSRIHSRLIGSRGANLDQL 3496
I N P K+ ITG ++ I A I+ + + ++ P+ + G K E +L + V I IP + + VTG + V+ EI + +E + + S+ + V HR ++G I + K + VP++DS +++T+ G+ ++ KA LA V + S ++ + ++ + +H LIG +GA L L
Sbjct: 171 IPNRDGPSSKITITGPREGIQRAAAHILAVSEREAKLATEHIVCPKNLVAFVRGPKNETYDRLTQNNGVKINIPPPHVTNEVISVTGEKDGVLRVAAEIRQIIESKKNVS--------SIQVAVARTQHRYIVGQSRSGIHDVLQKTGCVVEVPAEDSGSDQVTLIGNAQDLAKA----LALVIERASSVVTQSISAPNWLHKHLIGPKGATLTAL 374 HSP 9 Score: 50.8322 bits (120), Expect = 7.399e-6 Identity = 40/156 (25.64%), Postives = 74/156 (47.44%), Query Frame = 2
Query: 3170 ERMKQE----REIRNF----SLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
ER K E R IR+ S + +P +H +LIG +G + + + + RI +P++D ++ITI G ++ +++A +LA + E + + + + G + D+L + V+I P E ++ V G K+ V R A E+
Sbjct: 117 ERAKAEEARARIIRDLQTQASREIDIPKDHHGRLIGKEGALLRNLEAETNCRIQIPNRDGPSSKITITGPREGIQRAAAHILAVSEREAKLATEHIVCPKNLVAFVRGPKNETYDRLTQNNGVKINIPPPHVTNE---VISVTGEKDGVLRVAAEI 269
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Fly
Match: Dp1 (gene:FBgn0027835 transcript:FBtr0086681) HSP 1 Score: 297.36 bits (760), Expect = 4.137e-94 Identity = 217/691 (31.40%), Postives = 376/691 (54.41%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLR-FEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMC-KLNVXXXXXXXXXXEQGI----LEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
K YH + L+ + E R+++PP V K+EI ISG++D VA A + + Y M+ + +E+ K KH+Y+IGP+GS I EIL T V V++ N+ TI L GP+ LG ALT+V+ KS SV + I+A W+HK++IG+ G+N+ L + PN+ + ED +I +EG PE V YL + + + + + M ++P KHIIG G N+ +++ E +IN + + I G GV + L + I+ +EN K KD+I L +IG +G+ ++E+KDR+ +V I P P ++D++ L GP++DVD+ L K + ES+ +V I ++HK +IG+ G I+++ T TK +PA + N+ IVITGK+++V +A +R+ I + S+I+ ++I + +IG GG I+S+ E CGG V+I+FP+ D+ S+ V I+G KDDV KA+V+L +L E +++ + + ++FL ++K+R+ RI +E DK + IIG++E+V +KAR ++ I+ E ++ +++ K ++ F R + +++ + GI + +G I++A+ I I+ D E T +++ +R + I ++ +++ ++ VQI+ P
Sbjct: 249 KVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPP---NDSPSETITLRGPQVALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLED---KIKLEGDPENVDRAVAYLSEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNNIRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGG-VSIKFPNSDSKSDKVTIRGPKDDVEKAKVQLLELANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKEVITIIGKEESV-------KKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDKVTIKGAKDCIEAARQRIEEIVADLEAQTTIEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFP 925 HSP 2 Score: 69.707 bits (169), Expect = 4.137e-94 Identity = 39/133 (29.32%), Postives = 73/133 (54.89%), Query Frame = 3
Query: 123 IPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQK 512
+P +ER+ +SE + K++ +I G ++E +QSL I G++ ++ +A++ I TQ + +P+E R ++G G +E+E T+T+++ P +D S I I G+K+GI A Q I++
Sbjct: 100 VPCKERKSTESEKFGEGESKRICQQITKETGAQIEIVSGKNQSLTFLIKGKQSELLDARRKILMGFSTQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQ 232 HSP 3 Score: 138.658 bits (348), Expect = 1.502e-32 Identity = 173/734 (23.57%), Postives = 329/734 (44.82%), Query Frame = 2
Query: 1439 LIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXX-LHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEI-TASNYEQHFQMTLEDY--EFLFMSADLKLKKLRETY-SVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRD-NMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPS-QDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVD 3595
++G+ G+ L+EI +R RIN P +S+ I++ G ++ + Q +++ A + + S ++ +P YH I+G + +L + T + +P + DEIVI+G++ V A +V IY+ H +IG G+ I + + G V++ P D+ S T+ ++G QV L + + + SN + ++ + +++ +K+L E +V ++ EDK +++ G ENV + L SE+ E + + + + + G + N K + + Q + GP + ++ A+ E+ ID EN ++++I+D R ++I ++ E+ +Y QV I IP E N+ + G K ++ + S + +P G NI +I E + I + + + + ITG K+ + AKE I K+ ++ EV +P ++Y ++IG G+ I + V IK PN +SK KV + G + V AK +++E + ER++ +F+ + Q+H+ LIG G +I KIR RI PS +D++ ITI G +++V+KA+ ++ A + + + +++D + H + RG L ++ ++ V I FP G ++ V +KG K+ ++
Sbjct: 183 ILGKGGQRLREI-ERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKS-SDRITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTG--VSVEMPPNDSPSETITLRG-------PQVALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLEDK--IKLEGDPENVDRAVAYL----SEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNNIRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQE--------NTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEV---------IVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGGVSIKFPNSDSKSDKVTIRGPKDDVEKAKVQLLE-------LANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKEVITIIGKEESVKKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDK---VTIKGAKDCIE 872 HSP 4 Score: 127.872 bits (320), Expect = 2.829e-29 Identity = 90/334 (26.95%), Postives = 163/334 (48.80%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS-NINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSS----SASDQNLVNGGGDHIPMPSQTSSW 3883
+ ITG +K AK+ ++ L ++ E++VP + + +IG +G +++ + H V +++P K + V G+ V A++ +V+ +E+ E + +RE+R+F L + V ++H KLIG G I+K+R + V IS+P +D N I+I G++ N E A++ +L V E+ EV+ ID RIH LIG R + ++ + KV I F + + N + + G E V+ + L A + D + + + + + + ++GF I++APW+ N ++ S D GG + TS W
Sbjct: 972 IRITGRIEKCEAAKQALLDLIPIEEELSVPFDLHRTIIGPRGANVRQFMSKHDVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIEDNKVNIKF---SADDDNPNSIFISGKIEDVENVKELLFGMAEDYERDYLDNVAIAPPTIGAFLTG-----QGSGSDAAGASENGFVIKDAPWEKQKQAKNLTAPNTQSQEDFPHFAAGGAPVASTPITSVW 1297 HSP 5 Score: 110.538 bits (275), Expect = 6.486e-24 Identity = 137/534 (25.66%), Postives = 244/534 (45.69%), Query Frame = 2
Query: 746 ATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLV-------FTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLS------VNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQK--KHAFLVESNFSKKV-KIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLAL 2287
+T Q+ +P+E + I+G G ++EI T+ ++I Q I + G K+ + A + + KS I P H FI+G NL L +N P Q+ + ++IVI G + V ++ D++ + S + +E+ +++IG G I +I + T S+ P D + +T+ G P A+ L + + K +I H + +IG++G +K++++ P V +N D I L G ++VD+ A L + K+ E NF+ +V + Y+K IIG+ G + L I +N+ I I G ++ V A + +K N + I + LH +IG G +I +K+ +VTI P+P +++ V ++G K+DV K L LV+EI S++ + + ++F+ +KK+R+ +I +E + + I G+KENVL E +QK ++E+ IVTE + +
Sbjct: 166 STQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQ---SDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSD----RITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQV----QKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPPNDSPSETITLRG-PQ--VALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVN-----CLEDKIKLEGDPENVDRAVAYLSEIIKN---YEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNN-IRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRY-RQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQI 675 HSP 6 Score: 92.8189 bits (229), Expect = 1.615e-18 Identity = 114/513 (22.22%), Postives = 239/513 (46.59%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQ-TYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLG---PKDNLGPALTLVFTKSE-SVNIIAIDAPGWLHKFIIGKSGSNLVD-LSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKET-NCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMP-------QLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXX-XXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEIT 2050
+ + ++ K L + + R++IP S + I+I+G ++G+AQA + Q + Q K + +I +PK H +I+GP + ++ ++T +++ Q++ + + I + G + ++ E + ++++ H+++IG GS + + L + ++++ D ++ + P+ + + + V+ + + +E+ + K++IG G N++++ ++ N ++N D K + G P V+ A L E K + FT +M +IG+ G + +KD +V IN + ++ I + GP++ V Q LQ+K L E+ SK V I + H++IIG +G IRE+ + IP E D + + G ++DV+ K + + ++ I I K H +IGKGG I +++ + I P+ + ++I G K++VL+A+ ++ + E++
Sbjct: 177 REHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSD--RITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQK---DEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPPNDSPSETITLRGPQVALGNALTV---VYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLEDKIK----LEGDPENVDRAVAYL------SEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDEL-KVNINIEEREGQNN-IRIEGPKEGVRQAQLELQEKIDKL-ENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDET--QTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELS 666 HSP 7 Score: 86.6557 bits (213), Expect = 1.233e-16 Identity = 117/525 (22.29%), Postives = 223/525 (42.48%), Query Frame = 2
Query: 665 NEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDI--NEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFED---EANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP-RDLTK--------------------------------------KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIV-ITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHF 2074
++++I G +D V +A LL+ N+ + ++ ++ HK++IG G+ I++I D T + NE E I ++G ++++ A L + + + V + HK + K G L +S + + F ++++ I+G+ + + + +++ V DL+++ + +E+ I + I+G+ G +Q++ E + I FP RD T+ V+ I G EA K LL I E L +IG +G +++ + +V + PP + SD+I + G P + + A+++ + + +F +V + ++H +IGR G I +L + +P E ND I+ ITG Q + E A + I + IEI K +H LIG+ I + E +V I+F + D + N++ I G +DV + L + E +YE+ +
Sbjct: 713 DKVTIRGPKDDVEKAKVQLLELANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKE----VITIIGKEESVKKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDKVTIKGAKDCIEAARQRIEEIVADLEAQTT-IEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFPDRDATEPVEGLTNGGSGENGGENEGQEGEQEVEKEAEQEPVRQCDVIRITGRIEKCEAAKQALLDLIPIEEELS-----VPFDLHRTIIGPRGANVRQFMSKH-DVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIED--NKVNIKFSADDDNPNSIFISGKIEDVENVKELLFGMAE-----DYERDY 1219 HSP 8 Score: 78.5666 bits (192), Expect = 3.430e-14 Identity = 62/223 (27.80%), Postives = 114/223 (51.12%), Query Frame = 2
Query: 2972 EVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVP--AQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRAN 3628
+V + + ++IGAKGEKI++++ + QV I IP V + G E V K++++ V +EI+ S + VP Q+H+ +IG G I KIR + +I +P++ I I G K+NV +AK + N+L + E + I + ++ +IG+ G + + ++ V I FP +++ V ++G K+ V++ +L++ AN
Sbjct: 526 DVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLV---------KEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGGVSIKFP---NSDSKSDKVTIRGPKDDVEKAKVQLLELAN 736 HSP 9 Score: 65.0846 bits (157), Expect = 5.461e-10 Identity = 65/297 (21.89%), Postives = 130/297 (43.77%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLFVLDLE------VNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFV---------NKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEAN--IVKVKGTKESVDRCADELVD 3619
K+ I G KD I A++ I ++ V DLE V +P+ + ++GA+G K++++ V IK P++++ + + +TG E AAK+ +++ + E L VP HR +IG +G + + +K+ V + +P + + I + G V +A+ ++ + +L S +++V ++D+ HS+LIG GA +++L+ + V I P R +E N I+ + G + + + D +++
Sbjct: 861 KVTIKGAKDCIEAARQRIEEI-VADLEAQTTIEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFPDRDATEPVEGLTNGGSGENGGENEGQEGEQEVEKEAEQEPVRQCDVIRITGRIEKCEAAKQALLDLIPIEEE-------------LSVPFDLHRTIIGPRGANVRQFMSKHDVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQV-DVDTEFHSKLIGRHGAVINKLRADHDVIISLPKR----DEPNDRIISITGYQANAEAARDAILE 1138 HSP 10 Score: 61.6178 bits (148), Expect = 5.352e-9 Identity = 52/212 (24.53%), Postives = 103/212 (48.58%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDL--EVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFP 3529
I G + ++ A+ +I+ F +V VP E + ++G G++++++ I IP+++ + + + G+ E + A++EI + E + +R + VP YH ++G E ++K++ + RI+VP Q +EI I G KD V AK +V A +E V + + H +IG +G+ + ++ + V + P
Sbjct: 147 IKGKQSELLDARRKILMGFSTQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSDR--------ITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMP 350 HSP 11 Score: 51.6026 bits (122), Expect = 6.523e-6 Identity = 35/142 (24.65%), Postives = 70/142 (49.30%), Query Frame = 3
Query: 255 ISGQKDKVCEAKKYITRLVQ-----------TQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPS-TLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIF 644
+ G +V EA++ + ++++ F + +D+ EFH LIG G++ +L + +S PK ++P+ +I I G + E AR I + V + + R+V+EI+ I L+ + R +++N ++ F
Sbjct: 1041 VCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIEDNKVNIKF 1182
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Fly
Match: Dp1 (gene:FBgn0027835 transcript:FBtr0345226) HSP 1 Score: 297.36 bits (760), Expect = 4.137e-94 Identity = 217/691 (31.40%), Postives = 376/691 (54.41%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLR-FEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMC-KLNVXXXXXXXXXXEQGI----LEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
K YH + L+ + E R+++PP V K+EI ISG++D VA A + + Y M+ + +E+ K KH+Y+IGP+GS I EIL T V V++ N+ TI L GP+ LG ALT+V+ KS SV + I+A W+HK++IG+ G+N+ L + PN+ + ED +I +EG PE V YL + + + + + M ++P KHIIG G N+ +++ E +IN + + I G GV + L + I+ +EN K KD+I L +IG +G+ ++E+KDR+ +V I P P ++D++ L GP++DVD+ L K + ES+ +V I ++HK +IG+ G I+++ T TK +PA + N+ IVITGK+++V +A +R+ I + S+I+ ++I + +IG GG I+S+ E CGG V+I+FP+ D+ S+ V I+G KDDV KA+V+L +L E +++ + + ++FL ++K+R+ RI +E DK + IIG++E+V +KAR ++ I+ E ++ +++ K ++ F R + +++ + GI + +G I++A+ I I+ D E T +++ +R + I ++ +++ ++ VQI+ P
Sbjct: 249 KVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPP---NDSPSETITLRGPQVALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLED---KIKLEGDPENVDRAVAYLSEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNNIRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGG-VSIKFPNSDSKSDKVTIRGPKDDVEKAKVQLLELANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKEVITIIGKEESV-------KKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDKVTIKGAKDCIEAARQRIEEIVADLEAQTTIEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFP 925 HSP 2 Score: 69.707 bits (169), Expect = 4.137e-94 Identity = 39/133 (29.32%), Postives = 73/133 (54.89%), Query Frame = 3
Query: 123 IPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQK 512
+P +ER+ +SE + K++ +I G ++E +QSL I G++ ++ +A++ I TQ + +P+E R ++G G +E+E T+T+++ P +D S I I G+K+GI A Q I++
Sbjct: 100 VPCKERKSTESEKFGEGESKRICQQITKETGAQIEIVSGKNQSLTFLIKGKQSELLDARRKILMGFSTQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQ 232 HSP 3 Score: 138.658 bits (348), Expect = 1.502e-32 Identity = 173/734 (23.57%), Postives = 329/734 (44.82%), Query Frame = 2
Query: 1439 LIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXX-LHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEI-TASNYEQHFQMTLEDY--EFLFMSADLKLKKLRETY-SVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRD-NMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPS-QDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVD 3595
++G+ G+ L+EI +R RIN P +S+ I++ G ++ + Q +++ A + + S ++ +P YH I+G + +L + T + +P + DEIVI+G++ V A +V IY+ H +IG G+ I + + G V++ P D+ S T+ ++G QV L + + + SN + ++ + +++ +K+L E +V ++ EDK +++ G ENV + L SE+ E + + + + + G + N K + + Q + GP + ++ A+ E+ ID EN ++++I+D R ++I ++ E+ +Y QV I IP E N+ + G K ++ + S + +P G NI +I E + I + + + + ITG K+ + AKE I K+ ++ EV +P ++Y ++IG G+ I + V IK PN +SK KV + G + V AK +++E + ER++ +F+ + Q+H+ LIG G +I KIR RI PS +D++ ITI G +++V+KA+ ++ A + + + +++D + H + RG L ++ ++ V I FP G ++ V +KG K+ ++
Sbjct: 183 ILGKGGQRLREI-ERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKS-SDRITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTG--VSVEMPPNDSPSETITLRG-------PQVALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLEDK--IKLEGDPENVDRAVAYL----SEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNNIRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQE--------NTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEV---------IVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGGVSIKFPNSDSKSDKVTIRGPKDDVEKAKVQLLE-------LANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKEVITIIGKEESVKKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDK---VTIKGAKDCIE 872 HSP 4 Score: 127.872 bits (320), Expect = 2.829e-29 Identity = 90/334 (26.95%), Postives = 163/334 (48.80%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS-NINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSS----SASDQNLVNGGGDHIPMPSQTSSW 3883
+ ITG +K AK+ ++ L ++ E++VP + + +IG +G +++ + H V +++P K + V G+ V A++ +V+ +E+ E + +RE+R+F L + V ++H KLIG G I+K+R + V IS+P +D N I+I G++ N E A++ +L V E+ EV+ ID RIH LIG R + ++ + KV I F + + N + + G E V+ + L A + D + + + + + + ++GF I++APW+ N ++ S D GG + TS W
Sbjct: 972 IRITGRIEKCEAAKQALLDLIPIEEELSVPFDLHRTIIGPRGANVRQFMSKHDVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIEDNKVNIKF---SADDDNPNSIFISGKIEDVENVKELLFGMAEDYERDYLDNVAIAPPTIGAFLTG-----QGSGSDAAGASENGFVIKDAPWEKQKQAKNLTAPNTQSQEDFPHFAAGGAPVASTPITSVW 1297 HSP 5 Score: 110.538 bits (275), Expect = 6.486e-24 Identity = 137/534 (25.66%), Postives = 244/534 (45.69%), Query Frame = 2
Query: 746 ATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLV-------FTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLS------VNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQK--KHAFLVESNFSKKV-KIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLAL 2287
+T Q+ +P+E + I+G G ++EI T+ ++I Q I + G K+ + A + + KS I P H FI+G NL L +N P Q+ + ++IVI G + V ++ D++ + S + +E+ +++IG G I +I + T S+ P D + +T+ G P A+ L + + K +I H + +IG++G +K++++ P V +N D I L G ++VD+ A L + K+ E NF+ +V + Y+K IIG+ G + L I +N+ I I G ++ V A + +K N + I + LH +IG G +I +K+ +VTI P+P +++ V ++G K+DV K L LV+EI S++ + + ++F+ +KK+R+ +I +E + + I G+KENVL E +QK ++E+ IVTE + +
Sbjct: 166 STQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQ---SDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSD----RITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQV----QKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPPNDSPSETITLRG-PQ--VALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVN-----CLEDKIKLEGDPENVDRAVAYLSEIIKN---YEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNN-IRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRY-RQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQI 675 HSP 6 Score: 92.8189 bits (229), Expect = 1.615e-18 Identity = 114/513 (22.22%), Postives = 239/513 (46.59%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQ-TYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLG---PKDNLGPALTLVFTKSE-SVNIIAIDAPGWLHKFIIGKSGSNLVD-LSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKET-NCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMP-------QLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXX-XXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEIT 2050
+ + ++ K L + + R++IP S + I+I+G ++G+AQA + Q + Q K + +I +PK H +I+GP + ++ ++T +++ Q++ + + I + G + ++ E + ++++ H+++IG GS + + L + ++++ D ++ + P+ + + + V+ + + +E+ + K++IG G N++++ ++ N ++N D K + G P V+ A L E K + FT +M +IG+ G + +KD +V IN + ++ I + GP++ V Q LQ+K L E+ SK V I + H++IIG +G IRE+ + IP E D + + G ++DV+ K + + ++ I I K H +IGKGG I +++ + I P+ + ++I G K++VL+A+ ++ + E++
Sbjct: 177 REHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSD--RITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQK---DEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPPNDSPSETITLRGPQVALGNALTV---VYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLEDKIK----LEGDPENVDRAVAYL------SEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDEL-KVNINIEEREGQNN-IRIEGPKEGVRQAQLELQEKIDKL-ENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDET--QTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELS 666 HSP 7 Score: 86.6557 bits (213), Expect = 1.233e-16 Identity = 117/525 (22.29%), Postives = 223/525 (42.48%), Query Frame = 2
Query: 665 NEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDI--NEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFED---EANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP-RDLTK--------------------------------------KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIV-ITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHF 2074
++++I G +D V +A LL+ N+ + ++ ++ HK++IG G+ I++I D T + NE E I ++G ++++ A L + + + V + HK + K G L +S + + F ++++ I+G+ + + + +++ V DL+++ + +E+ I + I+G+ G +Q++ E + I FP RD T+ V+ I G EA K LL I E L +IG +G +++ + +V + PP + SD+I + G P + + A+++ + + +F +V + ++H +IGR G I +L + +P E ND I+ ITG Q + E A + I + IEI K +H LIG+ I + E +V I+F + D + N++ I G +DV + L + E +YE+ +
Sbjct: 713 DKVTIRGPKDDVEKAKVQLLELANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKE----VITIIGKEESVKKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDKVTIKGAKDCIEAARQRIEEIVADLEAQTT-IEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFPDRDATEPVEGLTNGGSGENGGENEGQEGEQEVEKEAEQEPVRQCDVIRITGRIEKCEAAKQALLDLIPIEEELS-----VPFDLHRTIIGPRGANVRQFMSKH-DVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIED--NKVNIKFSADDDNPNSIFISGKIEDVENVKELLFGMAE-----DYERDY 1219 HSP 8 Score: 78.5666 bits (192), Expect = 3.430e-14 Identity = 62/223 (27.80%), Postives = 114/223 (51.12%), Query Frame = 2
Query: 2972 EVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVP--AQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRAN 3628
+V + + ++IGAKGEKI++++ + QV I IP V + G E V K++++ V +EI+ S + VP Q+H+ +IG G I KIR + +I +P++ I I G K+NV +AK + N+L + E + I + ++ +IG+ G + + ++ V I FP +++ V ++G K+ V++ +L++ AN
Sbjct: 526 DVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLV---------KEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGGVSIKFP---NSDSKSDKVTIRGPKDDVEKAKVQLLELAN 736 HSP 9 Score: 65.0846 bits (157), Expect = 5.461e-10 Identity = 65/297 (21.89%), Postives = 130/297 (43.77%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLFVLDLE------VNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFV---------NKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEAN--IVKVKGTKESVDRCADELVD 3619
K+ I G KD I A++ I ++ V DLE V +P+ + ++GA+G K++++ V IK P++++ + + +TG E AAK+ +++ + E L VP HR +IG +G + + +K+ V + +P + + I + G V +A+ ++ + +L S +++V ++D+ HS+LIG GA +++L+ + V I P R +E N I+ + G + + + D +++
Sbjct: 861 KVTIKGAKDCIEAARQRIEEI-VADLEAQTTIEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFPDRDATEPVEGLTNGGSGENGGENEGQEGEQEVEKEAEQEPVRQCDVIRITGRIEKCEAAKQALLDLIPIEEE-------------LSVPFDLHRTIIGPRGANVRQFMSKHDVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQV-DVDTEFHSKLIGRHGAVINKLRADHDVIISLPKR----DEPNDRIISITGYQANAEAARDAILE 1138 HSP 10 Score: 61.6178 bits (148), Expect = 5.352e-9 Identity = 52/212 (24.53%), Postives = 103/212 (48.58%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDL--EVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFP 3529
I G + ++ A+ +I+ F +V VP E + ++G G++++++ I IP+++ + + + G+ E + A++EI + E + +R + VP YH ++G E ++K++ + RI+VP Q +EI I G KD V AK +V A +E V + + H +IG +G+ + ++ + V + P
Sbjct: 147 IKGKQSELLDARRKILMGFSTQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSDR--------ITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMP 350 HSP 11 Score: 51.6026 bits (122), Expect = 6.523e-6 Identity = 35/142 (24.65%), Postives = 70/142 (49.30%), Query Frame = 3
Query: 255 ISGQKDKVCEAKKYITRLVQ-----------TQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPS-TLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIF 644
+ G +V EA++ + ++++ F + +D+ EFH LIG G++ +L + +S PK ++P+ +I I G + E AR I + V + + R+V+EI+ I L+ + R +++N ++ F
Sbjct: 1041 VCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIEDNKVNIKF 1182
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Fly
Match: Dp1 (gene:FBgn0027835 transcript:FBtr0340329) HSP 1 Score: 297.36 bits (760), Expect = 4.137e-94 Identity = 217/691 (31.40%), Postives = 376/691 (54.41%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLR-FEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMC-KLNVXXXXXXXXXXEQGI----LEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
K YH + L+ + E R+++PP V K+EI ISG++D VA A + + Y M+ + +E+ K KH+Y+IGP+GS I EIL T V V++ N+ TI L GP+ LG ALT+V+ KS SV + I+A W+HK++IG+ G+N+ L + PN+ + ED +I +EG PE V YL + + + + + M ++P KHIIG G N+ +++ E +IN + + I G GV + L + I+ +EN K KD+I L +IG +G+ ++E+KDR+ +V I P P ++D++ L GP++DVD+ L K + ES+ +V I ++HK +IG+ G I+++ T TK +PA + N+ IVITGK+++V +A +R+ I + S+I+ ++I + +IG GG I+S+ E CGG V+I+FP+ D+ S+ V I+G KDDV KA+V+L +L E +++ + + ++FL ++K+R+ RI +E DK + IIG++E+V +KAR ++ I+ E ++ +++ K ++ F R + +++ + GI + +G I++A+ I I+ D E T +++ +R + I ++ +++ ++ VQI+ P
Sbjct: 249 KVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPP---NDSPSETITLRGPQVALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLED---KIKLEGDPENVDRAVAYLSEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNNIRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGG-VSIKFPNSDSKSDKVTIRGPKDDVEKAKVQLLELANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKEVITIIGKEESV-------KKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDKVTIKGAKDCIEAARQRIEEIVADLEAQTTIEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFP 925 HSP 2 Score: 69.707 bits (169), Expect = 4.137e-94 Identity = 39/133 (29.32%), Postives = 73/133 (54.89%), Query Frame = 3
Query: 123 IPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQK 512
+P +ER+ +SE + K++ +I G ++E +QSL I G++ ++ +A++ I TQ + +P+E R ++G G +E+E T+T+++ P +D S I I G+K+GI A Q I++
Sbjct: 100 VPCKERKSTESEKFGEGESKRICQQITKETGAQIEIVSGKNQSLTFLIKGKQSELLDARRKILMGFSTQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQ 232 HSP 3 Score: 138.658 bits (348), Expect = 1.502e-32 Identity = 173/734 (23.57%), Postives = 329/734 (44.82%), Query Frame = 2
Query: 1439 LIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXX-LHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEI-TASNYEQHFQMTLEDY--EFLFMSADLKLKKLRETY-SVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRD-NMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPS-QDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVD 3595
++G+ G+ L+EI +R RIN P +S+ I++ G ++ + Q +++ A + + S ++ +P YH I+G + +L + T + +P + DEIVI+G++ V A +V IY+ H +IG G+ I + + G V++ P D+ S T+ ++G QV L + + + SN + ++ + +++ +K+L E +V ++ EDK +++ G ENV + L SE+ E + + + + + G + N K + + Q + GP + ++ A+ E+ ID EN ++++I+D R ++I ++ E+ +Y QV I IP E N+ + G K ++ + S + +P G NI +I E + I + + + + ITG K+ + AKE I K+ ++ EV +P ++Y ++IG G+ I + V IK PN +SK KV + G + V AK +++E + ER++ +F+ + Q+H+ LIG G +I KIR RI PS +D++ ITI G +++V+KA+ ++ A + + + +++D + H + RG L ++ ++ V I FP G ++ V +KG K+ ++
Sbjct: 183 ILGKGGQRLREI-ERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKS-SDRITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTG--VSVEMPPNDSPSETITLRG-------PQVALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLEDK--IKLEGDPENVDRAVAYL----SEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNNIRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQE--------NTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEV---------IVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGGVSIKFPNSDSKSDKVTIRGPKDDVEKAKVQLLE-------LANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKEVITIIGKEESVKKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDK---VTIKGAKDCIE 872 HSP 4 Score: 127.872 bits (320), Expect = 2.829e-29 Identity = 90/334 (26.95%), Postives = 163/334 (48.80%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS-NINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSS----SASDQNLVNGGGDHIPMPSQTSSW 3883
+ ITG +K AK+ ++ L ++ E++VP + + +IG +G +++ + H V +++P K + V G+ V A++ +V+ +E+ E + +RE+R+F L + V ++H KLIG G I+K+R + V IS+P +D N I+I G++ N E A++ +L V E+ EV+ ID RIH LIG R + ++ + KV I F + + N + + G E V+ + L A + D + + + + + + ++GF I++APW+ N ++ S D GG + TS W
Sbjct: 972 IRITGRIEKCEAAKQALLDLIPIEEELSVPFDLHRTIIGPRGANVRQFMSKHDVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIEDNKVNIKF---SADDDNPNSIFISGKIEDVENVKELLFGMAEDYERDYLDNVAIAPPTIGAFLTG-----QGSGSDAAGASENGFVIKDAPWEKQKQAKNLTAPNTQSQEDFPHFAAGGAPVASTPITSVW 1297 HSP 5 Score: 110.538 bits (275), Expect = 6.486e-24 Identity = 137/534 (25.66%), Postives = 244/534 (45.69%), Query Frame = 2
Query: 746 ATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLV-------FTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLS------VNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQK--KHAFLVESNFSKKV-KIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLAL 2287
+T Q+ +P+E + I+G G ++EI T+ ++I Q I + G K+ + A + + KS I P H FI+G NL L +N P Q+ + ++IVI G + V ++ D++ + S + +E+ +++IG G I +I + T S+ P D + +T+ G P A+ L + + K +I H + +IG++G +K++++ P V +N D I L G ++VD+ A L + K+ E NF+ +V + Y+K IIG+ G + L I +N+ I I G ++ V A + +K N + I + LH +IG G +I +K+ +VTI P+P +++ V ++G K+DV K L LV+EI S++ + + ++F+ +KK+R+ +I +E + + I G+KENVL E +QK ++E+ IVTE + +
Sbjct: 166 STQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQ---SDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSD----RITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQV----QKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPPNDSPSETITLRG-PQ--VALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVN-----CLEDKIKLEGDPENVDRAVAYLSEIIKN---YEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNN-IRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRY-RQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQI 675 HSP 6 Score: 92.8189 bits (229), Expect = 1.615e-18 Identity = 114/513 (22.22%), Postives = 239/513 (46.59%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQ-TYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLG---PKDNLGPALTLVFTKSE-SVNIIAIDAPGWLHKFIIGKSGSNLVD-LSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKET-NCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMP-------QLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXX-XXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEIT 2050
+ + ++ K L + + R++IP S + I+I+G ++G+AQA + Q + Q K + +I +PK H +I+GP + ++ ++T +++ Q++ + + I + G + ++ E + ++++ H+++IG GS + + L + ++++ D ++ + P+ + + + V+ + + +E+ + K++IG G N++++ ++ N ++N D K + G P V+ A L E K + FT +M +IG+ G + +KD +V IN + ++ I + GP++ V Q LQ+K L E+ SK V I + H++IIG +G IRE+ + IP E D + + G ++DV+ K + + ++ I I K H +IGKGG I +++ + I P+ + ++I G K++VL+A+ ++ + E++
Sbjct: 177 REHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSD--RITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQK---DEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPPNDSPSETITLRGPQVALGNALTV---VYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLEDKIK----LEGDPENVDRAVAYL------SEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDEL-KVNINIEEREGQNN-IRIEGPKEGVRQAQLELQEKIDKL-ENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDET--QTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELS 666 HSP 7 Score: 86.6557 bits (213), Expect = 1.233e-16 Identity = 117/525 (22.29%), Postives = 223/525 (42.48%), Query Frame = 2
Query: 665 NEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDI--NEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFED---EANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP-RDLTK--------------------------------------KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIV-ITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHF 2074
++++I G +D V +A LL+ N+ + ++ ++ HK++IG G+ I++I D T + NE E I ++G ++++ A L + + + V + HK + K G L +S + + F ++++ I+G+ + + + +++ V DL+++ + +E+ I + I+G+ G +Q++ E + I FP RD T+ V+ I G EA K LL I E L +IG +G +++ + +V + PP + SD+I + G P + + A+++ + + +F +V + ++H +IGR G I +L + +P E ND I+ ITG Q + E A + I + IEI K +H LIG+ I + E +V I+F + D + N++ I G +DV + L + E +YE+ +
Sbjct: 713 DKVTIRGPKDDVEKAKVQLLELANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKE----VITIIGKEESVKKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDKVTIKGAKDCIEAARQRIEEIVADLEAQTT-IEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFPDRDATEPVEGLTNGGSGENGGENEGQEGEQEVEKEAEQEPVRQCDVIRITGRIEKCEAAKQALLDLIPIEEELS-----VPFDLHRTIIGPRGANVRQFMSKH-DVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIED--NKVNIKFSADDDNPNSIFISGKIEDVENVKELLFGMAE-----DYERDY 1219 HSP 8 Score: 78.5666 bits (192), Expect = 3.430e-14 Identity = 62/223 (27.80%), Postives = 114/223 (51.12%), Query Frame = 2
Query: 2972 EVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVP--AQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRAN 3628
+V + + ++IGAKGEKI++++ + QV I IP V + G E V K++++ V +EI+ S + VP Q+H+ +IG G I KIR + +I +P++ I I G K+NV +AK + N+L + E + I + ++ +IG+ G + + ++ V I FP +++ V ++G K+ V++ +L++ AN
Sbjct: 526 DVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLV---------KEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGGVSIKFP---NSDSKSDKVTIRGPKDDVEKAKVQLLELAN 736 HSP 9 Score: 65.0846 bits (157), Expect = 5.461e-10 Identity = 65/297 (21.89%), Postives = 130/297 (43.77%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLFVLDLE------VNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFV---------NKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEAN--IVKVKGTKESVDRCADELVD 3619
K+ I G KD I A++ I ++ V DLE V +P+ + ++GA+G K++++ V IK P++++ + + +TG E AAK+ +++ + E L VP HR +IG +G + + +K+ V + +P + + I + G V +A+ ++ + +L S +++V ++D+ HS+LIG GA +++L+ + V I P R +E N I+ + G + + + D +++
Sbjct: 861 KVTIKGAKDCIEAARQRIEEI-VADLEAQTTIEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFPDRDATEPVEGLTNGGSGENGGENEGQEGEQEVEKEAEQEPVRQCDVIRITGRIEKCEAAKQALLDLIPIEEE-------------LSVPFDLHRTIIGPRGANVRQFMSKHDVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQV-DVDTEFHSKLIGRHGAVINKLRADHDVIISLPKR----DEPNDRIISITGYQANAEAARDAILE 1138 HSP 10 Score: 61.6178 bits (148), Expect = 5.352e-9 Identity = 52/212 (24.53%), Postives = 103/212 (48.58%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDL--EVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFP 3529
I G + ++ A+ +I+ F +V VP E + ++G G++++++ I IP+++ + + + G+ E + A++EI + E + +R + VP YH ++G E ++K++ + RI+VP Q +EI I G KD V AK +V A +E V + + H +IG +G+ + ++ + V + P
Sbjct: 147 IKGKQSELLDARRKILMGFSTQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSDR--------ITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMP 350 HSP 11 Score: 51.6026 bits (122), Expect = 6.523e-6 Identity = 35/142 (24.65%), Postives = 70/142 (49.30%), Query Frame = 3
Query: 255 ISGQKDKVCEAKKYITRLVQ-----------TQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPS-TLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIF 644
+ G +V EA++ + ++++ F + +D+ EFH LIG G++ +L + +S PK ++P+ +I I G + E AR I + V + + R+V+EI+ I L+ + R +++N ++ F
Sbjct: 1041 VCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIEDNKVNIKF 1182
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Fly
Match: Dp1 (gene:FBgn0027835 transcript:FBtr0086682) HSP 1 Score: 297.36 bits (760), Expect = 4.137e-94 Identity = 217/691 (31.40%), Postives = 376/691 (54.41%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLR-FEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMC-KLNVXXXXXXXXXXEQGI----LEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
K YH + L+ + E R+++PP V K+EI ISG++D VA A + + Y M+ + +E+ K KH+Y+IGP+GS I EIL T V V++ N+ TI L GP+ LG ALT+V+ KS SV + I+A W+HK++IG+ G+N+ L + PN+ + ED +I +EG PE V YL + + + + + M ++P KHIIG G N+ +++ E +IN + + I G GV + L + I+ +EN K KD+I L +IG +G+ ++E+KDR+ +V I P P ++D++ L GP++DVD+ L K + ES+ +V I ++HK +IG+ G I+++ T TK +PA + N+ IVITGK+++V +A +R+ I + S+I+ ++I + +IG GG I+S+ E CGG V+I+FP+ D+ S+ V I+G KDDV KA+V+L +L E +++ + + ++FL ++K+R+ RI +E DK + IIG++E+V +KAR ++ I+ E ++ +++ K ++ F R + +++ + GI + +G I++A+ I I+ D E T +++ +R + I ++ +++ ++ VQI+ P
Sbjct: 249 KVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPP---NDSPSETITLRGPQVALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLED---KIKLEGDPENVDRAVAYLSEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNNIRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGG-VSIKFPNSDSKSDKVTIRGPKDDVEKAKVQLLELANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKEVITIIGKEESV-------KKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDKVTIKGAKDCIEAARQRIEEIVADLEAQTTIEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFP 925 HSP 2 Score: 69.707 bits (169), Expect = 4.137e-94 Identity = 39/133 (29.32%), Postives = 73/133 (54.89%), Query Frame = 3
Query: 123 IPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQK 512
+P +ER+ +SE + K++ +I G ++E +QSL I G++ ++ +A++ I TQ + +P+E R ++G G +E+E T+T+++ P +D S I I G+K+GI A Q I++
Sbjct: 100 VPCKERKSTESEKFGEGESKRICQQITKETGAQIEIVSGKNQSLTFLIKGKQSELLDARRKILMGFSTQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQ 232 HSP 3 Score: 138.658 bits (348), Expect = 1.502e-32 Identity = 173/734 (23.57%), Postives = 329/734 (44.82%), Query Frame = 2
Query: 1439 LIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXX-LHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEI-TASNYEQHFQMTLEDY--EFLFMSADLKLKKLRETY-SVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRD-NMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPS-QDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVD 3595
++G+ G+ L+EI +R RIN P +S+ I++ G ++ + Q +++ A + + S ++ +P YH I+G + +L + T + +P + DEIVI+G++ V A +V IY+ H +IG G+ I + + G V++ P D+ S T+ ++G QV L + + + SN + ++ + +++ +K+L E +V ++ EDK +++ G ENV + L SE+ E + + + + + G + N K + + Q + GP + ++ A+ E+ ID EN ++++I+D R ++I ++ E+ +Y QV I IP E N+ + G K ++ + S + +P G NI +I E + I + + + + ITG K+ + AKE I K+ ++ EV +P ++Y ++IG G+ I + V IK PN +SK KV + G + V AK +++E + ER++ +F+ + Q+H+ LIG G +I KIR RI PS +D++ ITI G +++V+KA+ ++ A + + + +++D + H + RG L ++ ++ V I FP G ++ V +KG K+ ++
Sbjct: 183 ILGKGGQRLREI-ERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKS-SDRITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTG--VSVEMPPNDSPSETITLRG-------PQVALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLEDK--IKLEGDPENVDRAVAYL----SEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNNIRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQE--------NTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEV---------IVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGGVSIKFPNSDSKSDKVTIRGPKDDVEKAKVQLLE-------LANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKEVITIIGKEESVKKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDK---VTIKGAKDCIE 872 HSP 4 Score: 127.872 bits (320), Expect = 2.829e-29 Identity = 90/334 (26.95%), Postives = 163/334 (48.80%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS-NINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSS----SASDQNLVNGGGDHIPMPSQTSSW 3883
+ ITG +K AK+ ++ L ++ E++VP + + +IG +G +++ + H V +++P K + V G+ V A++ +V+ +E+ E + +RE+R+F L + V ++H KLIG G I+K+R + V IS+P +D N I+I G++ N E A++ +L V E+ EV+ ID RIH LIG R + ++ + KV I F + + N + + G E V+ + L A + D + + + + + + ++GF I++APW+ N ++ S D GG + TS W
Sbjct: 972 IRITGRIEKCEAAKQALLDLIPIEEELSVPFDLHRTIIGPRGANVRQFMSKHDVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIEDNKVNIKF---SADDDNPNSIFISGKIEDVENVKELLFGMAEDYERDYLDNVAIAPPTIGAFLTG-----QGSGSDAAGASENGFVIKDAPWEKQKQAKNLTAPNTQSQEDFPHFAAGGAPVASTPITSVW 1297 HSP 5 Score: 110.538 bits (275), Expect = 6.486e-24 Identity = 137/534 (25.66%), Postives = 244/534 (45.69%), Query Frame = 2
Query: 746 ATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLV-------FTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLS------VNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQK--KHAFLVESNFSKKV-KIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLAL 2287
+T Q+ +P+E + I+G G ++EI T+ ++I Q I + G K+ + A + + KS I P H FI+G NL L +N P Q+ + ++IVI G + V ++ D++ + S + +E+ +++IG G I +I + T S+ P D + +T+ G P A+ L + + K +I H + +IG++G +K++++ P V +N D I L G ++VD+ A L + K+ E NF+ +V + Y+K IIG+ G + L I +N+ I I G ++ V A + +K N + I + LH +IG G +I +K+ +VTI P+P +++ V ++G K+DV K L LV+EI S++ + + ++F+ +KK+R+ +I +E + + I G+KENVL E +QK ++E+ IVTE + +
Sbjct: 166 STQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQ---SDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSD----RITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQV----QKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPPNDSPSETITLRG-PQ--VALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVN-----CLEDKIKLEGDPENVDRAVAYLSEIIKN---YEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNN-IRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRY-RQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQI 675 HSP 6 Score: 92.8189 bits (229), Expect = 1.615e-18 Identity = 114/513 (22.22%), Postives = 239/513 (46.59%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQ-TYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLG---PKDNLGPALTLVFTKSE-SVNIIAIDAPGWLHKFIIGKSGSNLVD-LSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKET-NCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMP-------QLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXX-XXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEIT 2050
+ + ++ K L + + R++IP S + I+I+G ++G+AQA + Q + Q K + +I +PK H +I+GP + ++ ++T +++ Q++ + + I + G + ++ E + ++++ H+++IG GS + + L + ++++ D ++ + P+ + + + V+ + + +E+ + K++IG G N++++ ++ N ++N D K + G P V+ A L E K + FT +M +IG+ G + +KD +V IN + ++ I + GP++ V Q LQ+K L E+ SK V I + H++IIG +G IRE+ + IP E D + + G ++DV+ K + + ++ I I K H +IGKGG I +++ + I P+ + ++I G K++VL+A+ ++ + E++
Sbjct: 177 REHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSD--RITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQK---DEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPPNDSPSETITLRGPQVALGNALTV---VYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLEDKIK----LEGDPENVDRAVAYL------SEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDEL-KVNINIEEREGQNN-IRIEGPKEGVRQAQLELQEKIDKL-ENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDET--QTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELS 666 HSP 7 Score: 86.6557 bits (213), Expect = 1.233e-16 Identity = 117/525 (22.29%), Postives = 223/525 (42.48%), Query Frame = 2
Query: 665 NEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDI--NEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFED---EANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP-RDLTK--------------------------------------KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIV-ITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHF 2074
++++I G +D V +A LL+ N+ + ++ ++ HK++IG G+ I++I D T + NE E I ++G ++++ A L + + + V + HK + K G L +S + + F ++++ I+G+ + + + +++ V DL+++ + +E+ I + I+G+ G +Q++ E + I FP RD T+ V+ I G EA K LL I E L +IG +G +++ + +V + PP + SD+I + G P + + A+++ + + +F +V + ++H +IGR G I +L + +P E ND I+ ITG Q + E A + I + IEI K +H LIG+ I + E +V I+F + D + N++ I G +DV + L + E +YE+ +
Sbjct: 713 DKVTIRGPKDDVEKAKVQLLELANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKE----VITIIGKEESVKKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDKVTIKGAKDCIEAARQRIEEIVADLEAQTT-IEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFPDRDATEPVEGLTNGGSGENGGENEGQEGEQEVEKEAEQEPVRQCDVIRITGRIEKCEAAKQALLDLIPIEEELS-----VPFDLHRTIIGPRGANVRQFMSKH-DVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIED--NKVNIKFSADDDNPNSIFISGKIEDVENVKELLFGMAE-----DYERDY 1219 HSP 8 Score: 78.5666 bits (192), Expect = 3.430e-14 Identity = 62/223 (27.80%), Postives = 114/223 (51.12%), Query Frame = 2
Query: 2972 EVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVP--AQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRAN 3628
+V + + ++IGAKGEKI++++ + QV I IP V + G E V K++++ V +EI+ S + VP Q+H+ +IG G I KIR + +I +P++ I I G K+NV +AK + N+L + E + I + ++ +IG+ G + + ++ V I FP +++ V ++G K+ V++ +L++ AN
Sbjct: 526 DVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLV---------KEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGGVSIKFP---NSDSKSDKVTIRGPKDDVEKAKVQLLELAN 736 HSP 9 Score: 65.0846 bits (157), Expect = 5.461e-10 Identity = 65/297 (21.89%), Postives = 130/297 (43.77%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLFVLDLE------VNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFV---------NKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEAN--IVKVKGTKESVDRCADELVD 3619
K+ I G KD I A++ I ++ V DLE V +P+ + ++GA+G K++++ V IK P++++ + + +TG E AAK+ +++ + E L VP HR +IG +G + + +K+ V + +P + + I + G V +A+ ++ + +L S +++V ++D+ HS+LIG GA +++L+ + V I P R +E N I+ + G + + + D +++
Sbjct: 861 KVTIKGAKDCIEAARQRIEEI-VADLEAQTTIEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFPDRDATEPVEGLTNGGSGENGGENEGQEGEQEVEKEAEQEPVRQCDVIRITGRIEKCEAAKQALLDLIPIEEE-------------LSVPFDLHRTIIGPRGANVRQFMSKHDVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQV-DVDTEFHSKLIGRHGAVINKLRADHDVIISLPKR----DEPNDRIISITGYQANAEAARDAILE 1138 HSP 10 Score: 61.6178 bits (148), Expect = 5.352e-9 Identity = 52/212 (24.53%), Postives = 103/212 (48.58%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDL--EVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFP 3529
I G + ++ A+ +I+ F +V VP E + ++G G++++++ I IP+++ + + + G+ E + A++EI + E + +R + VP YH ++G E ++K++ + RI+VP Q +EI I G KD V AK +V A +E V + + H +IG +G+ + ++ + V + P
Sbjct: 147 IKGKQSELLDARRKILMGFSTQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSDR--------ITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMP 350 HSP 11 Score: 51.6026 bits (122), Expect = 6.523e-6 Identity = 35/142 (24.65%), Postives = 70/142 (49.30%), Query Frame = 3
Query: 255 ISGQKDKVCEAKKYITRLVQ-----------TQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPS-TLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIF 644
+ G +V EA++ + ++++ F + +D+ EFH LIG G++ +L + +S PK ++P+ +I I G + E AR I + V + + R+V+EI+ I L+ + R +++N ++ F
Sbjct: 1041 VCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIEDNKVNIKF 1182
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Fly
Match: Dp1 (gene:FBgn0027835 transcript:FBtr0086680) HSP 1 Score: 297.36 bits (760), Expect = 4.137e-94 Identity = 217/691 (31.40%), Postives = 376/691 (54.41%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLR-FEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMC-KLNVXXXXXXXXXXEQGI----LEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
K YH + L+ + E R+++PP V K+EI ISG++D VA A + + Y M+ + +E+ K KH+Y+IGP+GS I EIL T V V++ N+ TI L GP+ LG ALT+V+ KS SV + I+A W+HK++IG+ G+N+ L + PN+ + ED +I +EG PE V YL + + + + + M ++P KHIIG G N+ +++ E +IN + + I G GV + L + I+ +EN K KD+I L +IG +G+ ++E+KDR+ +V I P P ++D++ L GP++DVD+ L K + ES+ +V I ++HK +IG+ G I+++ T TK +PA + N+ IVITGK+++V +A +R+ I + S+I+ ++I + +IG GG I+S+ E CGG V+I+FP+ D+ S+ V I+G KDDV KA+V+L +L E +++ + + ++FL ++K+R+ RI +E DK + IIG++E+V +KAR ++ I+ E ++ +++ K ++ F R + +++ + GI + +G I++A+ I I+ D E T +++ +R + I ++ +++ ++ VQI+ P
Sbjct: 249 KVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPP---NDSPSETITLRGPQVALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLED---KIKLEGDPENVDRAVAYLSEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNNIRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGG-VSIKFPNSDSKSDKVTIRGPKDDVEKAKVQLLELANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKEVITIIGKEESV-------KKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDKVTIKGAKDCIEAARQRIEEIVADLEAQTTIEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFP 925 HSP 2 Score: 69.707 bits (169), Expect = 4.137e-94 Identity = 39/133 (29.32%), Postives = 73/133 (54.89%), Query Frame = 3
Query: 123 IPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQK 512
+P +ER+ +SE + K++ +I G ++E +QSL I G++ ++ +A++ I TQ + +P+E R ++G G +E+E T+T+++ P +D S I I G+K+GI A Q I++
Sbjct: 100 VPCKERKSTESEKFGEGESKRICQQITKETGAQIEIVSGKNQSLTFLIKGKQSELLDARRKILMGFSTQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQ 232 HSP 3 Score: 138.658 bits (348), Expect = 1.502e-32 Identity = 173/734 (23.57%), Postives = 329/734 (44.82%), Query Frame = 2
Query: 1439 LIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXX-LHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEI-TASNYEQHFQMTLEDY--EFLFMSADLKLKKLRETY-SVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRD-NMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPS-QDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVD 3595
++G+ G+ L+EI +R RIN P +S+ I++ G ++ + Q +++ A + + S ++ +P YH I+G + +L + T + +P + DEIVI+G++ V A +V IY+ H +IG G+ I + + G V++ P D+ S T+ ++G QV L + + + SN + ++ + +++ +K+L E +V ++ EDK +++ G ENV + L SE+ E + + + + + G + N K + + Q + GP + ++ A+ E+ ID EN ++++I+D R ++I ++ E+ +Y QV I IP E N+ + G K ++ + S + +P G NI +I E + I + + + + ITG K+ + AKE I K+ ++ EV +P ++Y ++IG G+ I + V IK PN +SK KV + G + V AK +++E + ER++ +F+ + Q+H+ LIG G +I KIR RI PS +D++ ITI G +++V+KA+ ++ A + + + +++D + H + RG L ++ ++ V I FP G ++ V +KG K+ ++
Sbjct: 183 ILGKGGQRLREI-ERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKS-SDRITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTG--VSVEMPPNDSPSETITLRG-------PQVALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLEDK--IKLEGDPENVDRAVAYL----SEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNNIRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQE--------NTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEV---------IVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGGVSIKFPNSDSKSDKVTIRGPKDDVEKAKVQLLE-------LANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKEVITIIGKEESVKKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDK---VTIKGAKDCIE 872 HSP 4 Score: 127.872 bits (320), Expect = 2.829e-29 Identity = 90/334 (26.95%), Postives = 163/334 (48.80%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS-NINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSS----SASDQNLVNGGGDHIPMPSQTSSW 3883
+ ITG +K AK+ ++ L ++ E++VP + + +IG +G +++ + H V +++P K + V G+ V A++ +V+ +E+ E + +RE+R+F L + V ++H KLIG G I+K+R + V IS+P +D N I+I G++ N E A++ +L V E+ EV+ ID RIH LIG R + ++ + KV I F + + N + + G E V+ + L A + D + + + + + + ++GF I++APW+ N ++ S D GG + TS W
Sbjct: 972 IRITGRIEKCEAAKQALLDLIPIEEELSVPFDLHRTIIGPRGANVRQFMSKHDVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIEDNKVNIKF---SADDDNPNSIFISGKIEDVENVKELLFGMAEDYERDYLDNVAIAPPTIGAFLTG-----QGSGSDAAGASENGFVIKDAPWEKQKQAKNLTAPNTQSQEDFPHFAAGGAPVASTPITSVW 1297 HSP 5 Score: 110.538 bits (275), Expect = 6.486e-24 Identity = 137/534 (25.66%), Postives = 244/534 (45.69%), Query Frame = 2
Query: 746 ATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLV-------FTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLS------VNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQK--KHAFLVESNFSKKV-KIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLAL 2287
+T Q+ +P+E + I+G G ++EI T+ ++I Q I + G K+ + A + + KS I P H FI+G NL L +N P Q+ + ++IVI G + V ++ D++ + S + +E+ +++IG G I +I + T S+ P D + +T+ G P A+ L + + K +I H + +IG++G +K++++ P V +N D I L G ++VD+ A L + K+ E NF+ +V + Y+K IIG+ G + L I +N+ I I G ++ V A + +K N + I + LH +IG G +I +K+ +VTI P+P +++ V ++G K+DV K L LV+EI S++ + + ++F+ +KK+R+ +I +E + + I G+KENVL E +QK ++E+ IVTE + +
Sbjct: 166 STQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQ---SDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSD----RITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQV----QKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPPNDSPSETITLRG-PQ--VALGNALTVVYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVN-----CLEDKIKLEGDPENVDRAVAYLSEIIKN---YEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDELKVNINIEEREGQNN-IRIEGPKEGVRQAQLELQEKIDKLENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRY-RQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQI 675 HSP 6 Score: 92.8189 bits (229), Expect = 1.615e-18 Identity = 114/513 (22.22%), Postives = 239/513 (46.59%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQ-TYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLG---PKDNLGPALTLVFTKSE-SVNIIAIDAPGWLHKFIIGKSGSNLVD-LSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKET-NCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMP-------QLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXX-XXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEIT 2050
+ + ++ K L + + R++IP S + I+I+G ++G+AQA + Q + Q K + +I +PK H +I+GP + ++ ++T +++ Q++ + + I + G + ++ E + ++++ H+++IG GS + + L + ++++ D ++ + P+ + + + V+ + + +E+ + K++IG G N++++ ++ N ++N D K + G P V+ A L E K + FT +M +IG+ G + +KD +V IN + ++ I + GP++ V Q LQ+K L E+ SK V I + H++IIG +G IRE+ + IP E D + + G ++DV+ K + + ++ I I K H +IGKGG I +++ + I P+ + ++I G K++VL+A+ ++ + E++
Sbjct: 177 REHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSD--RITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQK---DEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMPPNDSPSETITLRGPQVALGNALTV---VYQKSNSVKSVEINAAHWIHKYVIGRKGANMKQLEEDCPNVNVNCLEDKIK----LEGDPENVDRAVAYL------SEIIKNYEENFTFEVMTVNPSYYKHIIGKAGANVNRLKDEL-KVNINIEEREGQNN-IRIEGPKEGVRQAQLELQEKIDKL-ENEKSKDVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLVKEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDET--QTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELS 666 HSP 7 Score: 86.6557 bits (213), Expect = 1.233e-16 Identity = 117/525 (22.29%), Postives = 223/525 (42.48%), Query Frame = 2
Query: 665 NEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDI--NEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFED---EANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP-RDLTK--------------------------------------KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIV-ITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHF 2074
++++I G +D V +A LL+ N+ + ++ ++ HK++IG G+ I++I D T + NE E I ++G ++++ A L + + + V + HK + K G L +S + + F ++++ I+G+ + + + +++ V DL+++ + +E+ I + I+G+ G +Q++ E + I FP RD T+ V+ I G EA K LL I E L +IG +G +++ + +V + PP + SD+I + G P + + A+++ + + +F +V + ++H +IGR G I +L + +P E ND I+ ITG Q + E A + I + IEI K +H LIG+ I + E +V I+F + D + N++ I G +DV + L + E +YE+ +
Sbjct: 713 DKVTIRGPKDDVEKAKVQLLELANERQLASFTAEVRAKQQHHKFLIGKNGASIRKIRDATGARIIFPSNEDTDKE----VITIIGKEESVKKAREQLEAIIKECDEVTEGEVSVDPKHHKHFVAKRGFILHRISEECGGVMISFPRVGINSDKVTIKGAKDCIEAARQRIEEIVADLEAQTT-IEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFPDRDATEPVEGLTNGGSGENGGENEGQEGEQEVEKEAEQEPVRQCDVIRITGRIEKCEAAKQALLDLIPIEEELS-----VPFDLHRTIIGPRGANVRQFMSKH-DVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIED--NKVNIKFSADDDNPNSIFISGKIEDVENVKELLFGMAE-----DYERDY 1219 HSP 8 Score: 78.5666 bits (192), Expect = 3.430e-14 Identity = 62/223 (27.80%), Postives = 114/223 (51.12%), Query Frame = 2
Query: 2972 EVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVP--AQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRAN 3628
+V + + ++IGAKGEKI++++ + QV I IP V + G E V K++++ V +EI+ S + VP Q+H+ +IG G I KIR + +I +P++ I I G K+NV +AK + N+L + E + I + ++ +IG+ G + + ++ V I FP +++ V ++G K+ V++ +L++ AN
Sbjct: 526 DVIIDRRLHRSIIGAKGEKIREVKDRYRQVTITIPTPQENTDIVKLRGPKEDVDKCHKDLLKLV---------KEIQESSHIIEVPIFKQFHKFVIGKGGANIKKIRDETQTKIDLPAEGDTNEVIVITGKKENVLEAKERIQKIQNELSDIVTEEVQIPPKYYNSIIGTGGKLISSIMEECGGVSIKFP---NSDSKSDKVTIRGPKDDVEKAKVQLLELAN 736 HSP 9 Score: 65.0846 bits (157), Expect = 5.461e-10 Identity = 65/297 (21.89%), Postives = 130/297 (43.77%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLFVLDLE------VNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFV---------NKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEAN--IVKVKGTKESVDRCADELVD 3619
K+ I G KD I A++ I ++ V DLE V +P+ + ++GA+G K++++ V IK P++++ + + +TG E AAK+ +++ + E L VP HR +IG +G + + +K+ V + +P + + I + G V +A+ ++ + +L S +++V ++D+ HS+LIG GA +++L+ + V I P R +E N I+ + G + + + D +++
Sbjct: 861 KVTIKGAKDCIEAARQRIEEI-VADLEAQTTIEVVIPQRHHRTIMGARGFKVQQVTFEFDVQIKFPDRDATEPVEGLTNGGSGENGGENEGQEGEQEVEKEAEQEPVRQCDVIRITGRIEKCEAAKQALLDLIPIEEE-------------LSVPFDLHRTIIGPRGANVRQFMSKHDVHVELPPSELKSDVIKVCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQV-DVDTEFHSKLIGRHGAVINKLRADHDVIISLPKR----DEPNDRIISITGYQANAEAARDAILE 1138 HSP 10 Score: 61.6178 bits (148), Expect = 5.352e-9 Identity = 52/212 (24.53%), Postives = 103/212 (48.58%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDL--EVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFP 3529
I G + ++ A+ +I+ F +V VP E + ++G G++++++ I IP+++ + + + G+ E + A++EI + E + +R + VP YH ++G E ++K++ + RI+VP Q +EI I G KD V AK +V A +E V + + H +IG +G+ + ++ + V + P
Sbjct: 147 IKGKQSELLDARRKILMGFSTQASRQVTVPREHFRVILGKGGQRLREIERVTATRINIPSQSDESEFITIAGTKEGIAQAEQEIRQLSAEQYKKSSDR--------ITVPKVYHPFIVGPYSENLNKLQEETGARINVPPQQVQKDEIVISGEKDAVAAAKAKVEAIYKDMEKKCSTVSVEVAKPKHRYVIGPKGSTIAEILQLTGVSVEMP 350 HSP 11 Score: 51.6026 bits (122), Expect = 6.523e-6 Identity = 35/142 (24.65%), Postives = 70/142 (49.30%), Query Frame = 3
Query: 255 ISGQKDKVCEAKKYITRLVQ-----------TQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPS-TLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIF 644
+ G +V EA++ + ++++ F + +D+ EFH LIG G++ +L + +S PK ++P+ +I I G + E AR I + V + + R+V+EI+ I L+ + R +++N ++ F
Sbjct: 1041 VCGTPARVAEAREALVKMIEDYEADRADRELRSFVLQVDVDTEFHSKLIGRHGAVINKLRADHDVIISLPKRDEPNDRIISITGYQANAEAARDAILEIVGDPETLHREVIEIDKRIHPHLIGQRRRTIRKIIEDNKVNIKF 1182
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Zebrafish
Match: hdlbpa (high density lipoprotein binding protein a [Source:ZFIN;Acc:ZDB-GENE-030131-2032]) HSP 1 Score: 377.481 bits (968), Expect = 1.688e-127 Identity = 286/997 (28.69%), Postives = 503/997 (50.45%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI--SEAEDKSH--LRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP--------------------FYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIK-------------LFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFT-KVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIE 3427
K +H + +++ R+++PP SV+K EI I+G+++ VA A + + Y + K + +E+ K +HKY+IGP+G+ ++EIL++T V V+I + S T+ L G LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG ++V V ++ V DL SR+ E+ +DP +H+IG GG NI +I++ S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ +KEI+D+FPEV INFP P SD++ L GPR +V++ + +QK A +VE++FS V I ++HK IIG+ G I+++ + TNTK +PA + ++ IVITGK+ + E A R+ I ++ +NI E+ + I LH LIG G + S+ E CGG V I FP+ + + V I+G ++V KA+ +L L EE ++ + E ++FL ++K+R++ RI AEDK H + +IG +E V + L+ + IV + + + +F + ++ R D + V ++ + ++ + +G +++AK ++ +I+D + +T ++ ++ ++I +++ +I++ + VQI+ P EPV+ V G K + P L I+ + S K + E+N +V + L D IS ITG + AKE +++ L L + V +++P +IG KG I +R H+V+I+ P KN + ++ +TG ++ +AA+ I V+ELE M S + + ++ H ++IG +G+ I KI + V + P S ++ N +T+ G ++V++A + +L + + ++E
Sbjct: 234 KVFHPFITGAHGKLVGDLMQETGARINVPPPSVNKTEIVITGEKEQVALAMVKIKKLYEEKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILERTGVSVEIPPL---DSSSETVILRGEPARLGQALTEVYAKANSYTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTKDVQIVQGQIEVIVTDLVSRMDYTEISVDPKFHRHLIGKGGANINRIKELHKVSVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDMIIEQRFHRAIIGQKGEKIKEIRDKFPEVIINFPDPAQKSDIVQLRGPRTEVEKCSKFMQKMVAEMVENSFSVSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPAENSNSEMIVITGKKANCEAAKNRILAIQKELANITEVEVSIPSKLHNSLIGSKGRFVRSIMEECGG-VHIHFPTEGSGIDAVTIRGPAEEVEKAKKQLLSLAEEKQTKSHTVELRAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPTAEDKDHELITVIGTEEAVAEAQKELEALIKSLDNIVEDFMIVDPKHHRFFVARRGQVLRDIADEYGGVIVSFPRTAAQSDK---VTLKGAKDCVEAAKKRMLEMIEDLDAQVTMECVIPQKFHRSIMGPKGSRIQQITKDHNVQIKFPDREEQQQADASVQENGEANGEVKEPVDPTAPKKCDVIVLSGRKERCEAAVEALKALVPVTIAVEVPFELHRYIIGQKGSGIRKMMDEFEVNIQVPAH-----ELQSDIIS----------ITGLASHLDRAKEGLLERVKELQAEQEDRALRSFKLTITVEPKYHPKIIGRKGAIISHIRHDHEVNIQFPEKNDENQDQITITGYEQNAIAARDAIQAIVDELEEM--------ISEDITLDSRVHARIIGARGKGIRKIMDEFKVDVRFPQSGAADPNLVTVTGRPEHVDEAIDHLLNLEEEYMADVVE 1200 HSP 2 Score: 101.293 bits (251), Expect = 1.688e-127 Identity = 55/139 (39.57%), Postives = 82/139 (58.99%), Query Frame = 3
Query: 102 FITDTFVIPYEERRYID----SESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
IT F +P EER+Y D E ++ K L +I G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G +ELE T+T++ P+ +DPS IKI G+K+G+E A+ I
Sbjct: 78 IITQVFHVPLEERKYKDMNQFGEGDQAKVCL-DIMHKTGAHLELSMAKDQGLSIMVSGKYDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKSGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEI 215 HSP 3 Score: 160.229 bits (404), Expect = 4.825e-39 Identity = 101/310 (32.58%), Postives = 174/310 (56.13%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVD-RANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQ 3826
++G K++ A E + L + + V VP E + +IG KG I+K+ +V+I++P + + +TG + + AK+ ++E V+EL+ +++R +R+F L + V +YH K+IG KG IS IR + V I P + D N ++ITI G++ N A++ + A V++LE I E + +DSR+H+R+IG+RG + ++ ++KV++ FP G + N+V V G E VD D L++ M D + + ++ K S ++ EE P+K GF +R APW A GN+ S+S++
Sbjct: 952 LSGRKERCEAAVEALKALVPVTIAVEVPFELHRYIIGQKGSGIRKMMDEFEVNIQVPAHELQSDIISITGLASHLDRAKEGLLERVKELQAEQEDRALRSFKLTITVEPKYHPKIIGRKGAIISHIRHDHEVNIQFPEKNDENQDQITITGYEQNAIAARDAIQAIVDELEEMISEDITLDSRVHARIIGARGKGIRKIMDEFKVDVRFPQSG--AADPNLVTVTGRPEHVDEAIDHLLNLEEEYMADVVENEAKMAYMKPSGSSASSM-EEPRGPSK-------GFVVREAPW-ATGNEKAPDMSSSEE 1250 HSP 4 Score: 129.798 bits (325), Expect = 1.040e-29 Identity = 158/687 (23.00%), Postives = 299/687 (43.52%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXE-QGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLI-VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSST-ISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFV--NKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
+++ S V IP ++H+ +IG+ G ++EL T TK QIP + +++I I+G ++ +E A + I ++ + I K H + G G + L + G R+ + P P + ++I G K+ V A VK+ L EE + ++ ++++ L+++ R SV I + S I+ G+ + L EV KA S S++ ++ + F IG+ N+ K+ K E + + GP ++++ + +I I+ D + + I VD + +++ ++ I + ++V ++IP E N I + +K + N D++ E + G K I EI + I+ K I ++ P+ ++E ++ + ++ V+VP ++F+ +IG G IKK+R I +P +NS +++TG + AAK I+ +EL + + + + +P++ H LIG+KG + I + V I P++ S I+ +TI+G + VEKAK ++L+ + +S +E L H LIG G N+ +++ I+FP E ++ ++ V GT+E+V EL
Sbjct: 150 LQTQASATVAIPKEHHRFVIGKSGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEILLISAEQDKRAVERMNIDKVFHPFITGAHGKLVGDLMQETGARINV--PPPSVNKTEIVITGEKEQVALAMVKIKKLYEEKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILERTGVSVEIPPLDSSSETVILRGEPARLGQALTEVYAKANS--------YTVSSVSAPSWLHR----FIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTKDVQIVQGQIEVIVTDLVSRMDYTEISVDPKFHRHLIGKGGANINRIKELHKVSVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRME-------------NERTKDMIIEQRFHRAIIGQKGEKIKEIRDKFPEVIINFPDPAQKSDIVQLRGPRTEVEKCS-----KFMQKMVAEMVENSFSVSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPAENSNSEMIVITGKKANCEAAKNRILAIQKELANITE--------VEVSIPSKLHNSLIGSKGRFVRSIMEECGGVHIHFPTEGSGIDAVTIRGPAEEVEKAKKQLLSLAEEKQTKSHTVE-LRAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPT--AEDKDHELITVIGTEEAVAEAQKEL 793 HSP 5 Score: 121.709 bits (304), Expect = 2.884e-27 Identity = 229/1044 (21.93%), Postives = 438/1044 (41.95%), Query Frame = 2
Query: 773 IPKEKHKYI-IGPRGSGIKEILDQTSVWVDIN-------EQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQL-RFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVE-AVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAA----SEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXX-XLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLED--YEFLFMSADLKLKKLRETYSVRISEAEDKSH----LRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQG---ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQM--SEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-VLDLEVNVPEEF-------YPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ------DSNINE----------------------ITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQP 3706
+P E+ KY + G G DQ V +DI E + + +I + G D + A + ++ ++ + P H+F+IGKSG L +L + IQ+ R +D +NQI I G+ +E +E +++ + Q + + M ID + I G+ G + + +ET IN P + K + I G V A+ I E +N + + +IG +G L+EI +R V + PP D+ S+ + L G P + + + K +++ V S V P H+ IIG++G + ++T+ Q+P +E D+I + G KDV+ ++ I + ++ H LIGKGG IN +KE +V++R P + SN + I+G V +A+ +L +L + E+ M +E + + K+K++R+ + I D + +++ G + V + +QK +EM V + ++S+ F K+ + G N+ K+ P E ++ G N ++AK+ I+ I + N ITE + +L N S+ + S + + V I P +++ TI + +K ++ + + L P + I G NI ++ + I A +KD + + G ++ + A++E+ L LD N+ E+F + + +G+ ++ + + V + P ++ KV + G+ + V AAKK ++E +E+L+ ++ +P ++HR ++G KG I +I ++V+I P + D+++ E I + G K+ E A + A V + + + +H +IG +G+ + ++ +++V I P +++I+ + G +DR + L++R + E DR + E + P
Sbjct: 85 VPLEERKYKDMNQFGEG-----DQAKVCLDIMHKTGAHLELSMAKDQGLSIMVSGKYDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKSGEKLQELELKTATKIQIPRPDDPSNQIKISGT-KEGLEKAKHEILLISAEQDKRAVERMNIDKVFHPFITGAHGKLVGDLMQETGARINVPPPSVNKTEIVITGEKEQVALAMVKIKKLYEEKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILERTG-VSVEIPPLDSSSETVILRGEPARLGQALTEVYAKANSYTVSS-----VSAPSWLHRFIIGKKGQNLAKITQ------QMPKVHIEFTEGEDKITLEGPTKDVQIVQGQIEVIVTDLVSRMDYTEISVDPKFHRHLIGKGGANINRIKELH--KVSVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRM---ENERTKDMIIEQRFHRAIIGQKGEKIKEIRDKFPEVIINFPDPAQKSDIVQLRGPRTEVEKCSKFMQKMVAEM---VENSFSVSVPIFKQFHKN--IIGKGGANIKKIREETNTKIDLPAENSNSEMIVITGKKANCEAAKNRILAIQKELAN-ITEVEVSIPSKLHNSLIGSKGRFVRSIMEECGGVHIHFPTEGSGIDAVTIRGPAEEVEKAKKQLLS-LAEEKQTKSHTVELRAKPEYHKFLI-------GKGGGNIRKVRDSTGARIIFPTAEDKD--------HELITVIGTEEAVAEAQKELEALIKSLD---NIVEDFMIVDPKHHRFFVARRGQVLRDIADEYGGVIVSFPRTAAQSDKVTLKGAKDCVEAAKKRMLEMIEDLD--------AQVTMECVIPQKFHRSIMGPKGSRIQQITKDHNVQIKFPDREEQQQADASVQENGEANGEVKEPVDPTAPKKCDVIVLSGRKERCEAAVEALKALVP-----VTIAVEVPFELHRYIIGQKGSGIRKMMDEFEVNIQVPAHEL---QSDIISITGLASHLDRAKEGLLERVKELQAEQEDRALRSFKLTITVEPKYHP 1064 HSP 6 Score: 117.472 bits (293), Expect = 6.565e-26 Identity = 126/531 (23.73%), Postives = 240/531 (45.20%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDL--------SVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENV 2215
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++E+ +T+ + I R ++ S N I + G K+ L A + L+ + + + ++ H FI G G + DL +V P++ +IVI G E+V +KK + + + + +E+ K++IG G +Q+I + T S+ P D + + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP KDV V ++ LV ++ + K+H+ +IG+ G I + + +IP +E+++ I I G + V++A K + + + N + I + H +IG+ G +I +++ V I FP P S+ V ++G + +V K + +V E+ +++ + + ++ + +KK+RE + +I +E + + I G+K N
Sbjct: 130 IMVSGKYDAVMKARKEIVSRLQTQASAT-----VAIPKEHHRFVIGKSGEKLQELELKTATKIQI--PRPDDPS-NQIKISGTKEGLEKAKHEILLISAEQDKRAVERMNIDKVFHPFITGAHGKLVGDLMQETGARINVPPPSVN------KTEIVITGEKEQVALAMVKIKKLYEEKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILERTGVSVEIPPLDSSSETVILRGEPARLGQALTEVYAKANSYTVSSVSAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTKDVQIVQGQIEVIVTDLVSRMDYTEISVDPKFHRHLIGKGGANINRIKELHKVSVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDMIIEQRFHRAIIGQKGEKIKEIRDKF-PEVIINFPDPAQKSDIVQLRGPRTEVEKCSKFMQKMVAEMVENSFSVSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPAENSNSEMIVITGKKANC 639 HSP 7 Score: 90.1225 bits (222), Expect = 1.440e-17 Identity = 119/516 (23.06%), Postives = 233/516 (45.16%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAA-SILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALT----LVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDL------SVNKPNIQLRFEDEANQIVIEGSP----EEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKET-NCSINFPRDLTKKVVTIIGTPHGVEAVKA-ILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXX-XXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEIT 2050
K +H+ KS L + + + ++ IP N+I ISG ++G+ +A ILL + Q K + + I+ K H +I G G + +++ +T +++ +N+ I + G K+ + A+ L K ++ IA++ HK++IG G+ L ++ SV P + + + +++ G P + + EV Y K + + S + + + IIG G N+ KI ++ I F K +T+ G V+ V+ I + + + +I LIG+ G + IK+ +V + PP + S+LI + G + V + L + A +E+ +K + I ++H+AIIG++G I+E+ K P ++++D + + G + +VE K + + + I K H +IGKGG I ++E + I P+ +++S ++I G K + A+ ++ + +E+
Sbjct: 162 KEHHRFVIGKSGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEILLISAEQDKRAVERMNID--KVFHPFITGAHGKLVGDLMQETGARINVPPPSVNK---TEIVITGEKEQVALAMVKIKKLYEEKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILERTGVSVEIPPL----DSSSETVILRGEPARLGQALTEV--YAKANSYTVSS------VSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDK--ITLEGPTKDVQIVQGQIEVIVTDLVSRMDYTEISVDPKFHRHLIGKGGANINRIKE-LHKVSVRIPPDNEKSNLIRIEGDPQGVQEAKKELLEL-ASRMENERTKDMIIEQRFHRAIIGQKGEKIKEIRDKFPEVIINFPDPAQKSDIVQLRGPRTEVEKCSKFMQKMVAEMVENSFSVSVPIFKQFHKNIIGKGGANIKKIREETNTK--IDLPAENSNSEMIVITGKKANCEAAKNRILAIQKELA 654
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Zebrafish
Match: hdlbpb (high density lipoprotein binding protein b [Source:ZFIN;Acc:ZDB-GENE-030131-6822]) HSP 1 Score: 359.762 bits (922), Expect = 1.226e-122 Identity = 279/1007 (27.71%), Postives = 519/1007 (51.54%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTK-KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTIS--NSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYK-----LEITGHKDKITLAKEEII-------------KLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIV-TGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS-NINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIH--SRLIGSRGANLD 3490
K +H N + + + R+ IPP S+ K+EI I+G+++ VA A + + TY + K + +E+ K +HKYI+GP+G+ ++EIL+ T V V++ L+ S TI L G D LGPALT V+ K++SV + + AP WLH+FIIGK G N+ ++ P + + F D +I +EG EEV + +++ + DL R+ E+ ID +H+IG G NI +I+++ S+ P+D + +V I G P GV+ + L+ + +EN + KD+I +IGQ+G+ +KE++D+FPEV I FP SD++ L GP+ +V++ A LQK A LVES+FS V I ++HK IIG+ G I+++ + TNTK +P + ++ IVITGK+ + E A +R+ I ++ +++ E+ + I LH LIG G+ + S+ E CGG V I FP+ + + V I+G ++V +A+ +L L EE +N+ Q E +++L ++++R+ RI ++ +++ H+ I+G++E V+ L+ + ++ +++ + +F + ++ R + + V ++ ++ + + +GP + + +AK I I+ D E+ ++ +++ +R + I ++ +I+++++VQI+ P E + S N P + + + + E+ + + +P I E + + +Y I + S I + +D NI +PQ + ++ITG + AK ++ L + ++V +++P +IG KG I ++R + V+++ P+K + VIV +G + AK I + V L+ M S + + + H ++IG +G+ I KI + V I P S + N++T+ G +NV+ + +L + + E + + + S+ +G+ G + D
Sbjct: 228 KAFHPFIAGAYNRLVQELSQETGARISIPPPSIPKDEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTGVSVEMPP--LDSAS-ETIILRGEPDKLGPALTQVYAKAKSVIVAEVIAPAWLHRFIIGKKGQNISRITQQLPKVHIEFTDGEERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERCGLVRIEGDPQGVQLARKELMDMAQRMENERTKDLIIEQKFHRTIIGQKGEKIKEVRDKFPEVIIIFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEVIVITGKKSNCEAARERILAIEKELASMKEVEVSIPAKLHNSLIGSKGSLVRSIMEDCGG-VHIHFPAEGSGLDRVTIRGPAEEVERARKQLLQLAEEKQVNNFSVELQAKPEYHKYLIGRGGANIRRVRDRTGARIIFPSADDQEQEHITIMGKEEAVILAQRELETLIKNLDDVIEDSMIVEPRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGTHSDS---ITLKGPRECVDAAKKRIQEIVCDLESQVSMEVLIPQRYHRAIMGPKGCRIQQITREHEVQIKFPDRDESAAQEPTSQENGDTDLTPRK---CDIITVTGRAEKCELARAALLALVPVTIDVEVSYDLHRY-----IIGQKGSGIR---KMMEDYEVNIWVPQPEQQSDIIKITGQVASVERAKHGLLDRVKELQAEQEDRALRSYKVTLSVDPKYHPKIIGRKGAVISQIRKEYDVNVQFPDKGDEQQDVIVISGYERNANDAKSAIEQLVAALQEM--------VSEDVRLDRRVHARIIGARGKAIRKIMEEFKVDIRFPQPGSEDPNKVTVTGLPENVDNTIDHLLNLEEEYMLSVTETETMAAYMKPPSKTMGTGGNDED 1208 HSP 2 Score: 102.834 bits (255), Expect = 1.226e-122 Identity = 54/139 (38.85%), Postives = 83/139 (59.71%), Query Frame = 3
Query: 102 FITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQ 509
IT F +P EERRY D+ + K+ +I G +E ++ DQ L + ++G+ D V +A+K I +QTQ ++ IPKE HR +IG G +ELE T+T+++ P+ +DP+ I+I G+K+GIE AR IQ
Sbjct: 72 VITQVFHVPLEERRYKDNSQFGEGEEAKVCLDIMQKTGAHIELSLAKDQGLSIMVTGKLDSVMKARKEIVARLQTQASATVLIPKEHHRFVIGKNGEKLQELELKTATKIAIPRSDDPNANIRITGTKEGIEKARHEIQ 210 HSP 3 Score: 181.03 bits (458), Expect = 1.806e-45 Identity = 264/1149 (22.98%), Postives = 502/1149 (43.69%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAA-SILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFT----KSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKP-NIQLRFEDEANQ-IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKE-TNCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCN-IYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRE-TYSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTY-FQKSNEL--FRIGRDNMC------KLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQ-VQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTI-------SAGVALNKDPISNIKLPQYKLEI---TGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTH---------KSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSW 3883
K +H+ K+ L + + + ++ IP I I+G ++G+ +A I L + Q K + L E K H +I G ++E+ +T + I + + + I + G K+ + A+ + K I+++ HK+I+G G+ L ++ N ++++ D A++ I++ G P+++ +V+ + E+ + + IIG G NI +I ++ I F D +++ T +A I + + +I LIG+ G + IK+++ +V + P L+ + G + V Q+A A +E+ +K + I K+H+ IIG++G I+E+ K P +++D + + G + +VE K + I E + I + I K H +IGKGG I ++E + I P+ +++S ++I G K + A+ ++ + +E+ AS E + + + L S ++ + E V I + S L + + E +++AR ++ + E +N+ + Q E + IGR + ++ EQ + G + + A+ E+ +I + ++ I +++IV+ R ++ + E++++Y V + P +S T+ +K V S+ + P IM G K I +I E I SA + L K +I TG +K LA+ ++ L + ++V V + + +IG KG I+K+ ++V+I +P + + +TG SV AK +++ V+EL+ +++R +R++ + L V +YH K+IG KG IS+IR + V + P + D + I I G++ N AK+ + V L+ + E + +D R+H+R+IG+RG + ++ +++KV+I FP G+ E+ N V V G E+VD D L++ + + L ++ + +P P+K+ T GF +R+APW+A GN SSA + G P QTS+W
Sbjct: 156 KEHHRFVIGKNGEKLQELELKTATKIAIPRSDDPNANIRITGTKEGIEKARHEIQLISAEQDKRAVERLCFE--KAFHPFIAGAYNRLVQELSQETGARISIPPPSIPK---DEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTGVSVEMPPLDSASETIILRGEPDKLGPAL----TQVYAKAKSVIVAEVIAPAWLHRFIIGKKGQNISRITQQLPKVHIEFT-DGEERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRFHRHLIGKNGANINRIKEQY-KVSVRIPQDSERCGLVRIEGDPQGV-QLARKELMDMAQRMENERTKDLIIEQKFHRTIIGQKGEKIKEVRDKFPEVIIIFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTK--IDLPTENSNSEVIVITGKKSNCEAARERILAIEKEL-ASMKEVEVSIPAKLHNSLIGSKGSLVRSIMEDCGGVHIHFPAEGSGL----DRVTIRGPAEEVERARKQLLQLAEEK---QVNNFSVELQAKPEYHKYLIGRGGANIRRVRDRTGARIIFPSADDQEQEHITIMGKEEAVILAQRELETLIKNLDDVIEDSMIVEPRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGTHSDSITLKGPRECVDAAKKRIQEIVCDLESQVSMEVLI---PQRYHRAIM------GPKGCRIQQITREHEVQIKFPDRDESAAQEPTSQENGDTDLTPRKCDIITVTGRAEKCELARAALLALVPVTIDVEVSYDLHRYIIGQKGSGIRKMMEDYEVNIWVPQPEQQSDIIKITGQVASVERAKHGLLDRVKELQAEQEDRALRSYKVTLSVDPKYHPKIIGRKGAVISQIRKEYDVNVQFPDKGDEQQDVIVISGYERNANDAKSAIEQLVAALQEMVSEDVRLDRRVHARIIGARGKAIRKIMEEFKVDIRFPQPGS--EDPNKVTVTGLPENVDNTIDHLLN--------LEEEYMLSVTETETMAAYMKP-----PSKTMGTGGNDEDNRALAKGFVVRDAPWNAQGNKAPDVSSAEEFPTFGSG----ITPKQTSAW 1254 HSP 4 Score: 112.849 bits (281), Expect = 1.627e-24 Identity = 189/869 (21.75%), Postives = 383/869 (44.07%), Query Frame = 2
Query: 1013 IIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEI------TASNYEQHFQMTLEDYEFLFMSADLKLKKLRETY--SVRISEAEDKSHL-RIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK--------SNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDY-ENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPN-----PNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISA-GVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTK-VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGI-IEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIR 3535
I+ K+G++ ++LS+ K D+ I++ G + V++ K+ V LQ++ S + I + +IG G +Q++ +T I PR D + I GT G+E + + + + + F P + G + ++E+ RI+ PPP D I + G ++ V A ++ + + V++ HK I+G +G T++E+ + T ++P ++ I++ G+ + A+ +V Y K ++I + LH +IGK G I+ + + +V I F + + ++G ++V +AQ+++ ++++++ T N +Q F + L + +++E Y SVRI + ++ L RI G + V Q AR E+ + DL QK E + RD ++ + + + I++ RGP ++ + +I + E++ + ++ + ++ KNI + +I ++ +I +P SNS V G+K E + P L N ++ S GS +I E V A G L++ I+ P ++E K + LA+E+ + F ++L+ E++ LIG G I+++R I P+ + + + + + G E+V+ A++E+ ++ L+ + ++ I V ++HR + +G+ + ++ + V +S P ++ + IT++G ++ V+ AK + V LES + +EVL I R H ++G +G + Q+ ++++V+I FP R
Sbjct: 104 IMQKTGAH-IELSLAK--------DQGLSIMVTGKLDSVMKAR---KEIVARLQTQASATVL-IPKEHHRFVIGKNGEKLQELELKTATKIAIPRSDDPNANIRITGTKEGIEKARHEIQLISAEQDKRAVERLCFEKAFHPFIAGAYNRLVQELSQETG-ARISIPPPSIPKDEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTGVSVEMPPLDSASETIILRGEPDKLGPALTQV---YAKAKSVIVAEVIAPAWLHRFIIGKKGQNISRITQQLP-KVHIEFTDGE---ERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRF------HRHLIGKNGANINRIKEQYKVSVRIPQDSERCGLVRIEGDPQGV-------QLARKELMDMAQRMENERTKDLIIEQKFHRTIIGQKGEKIKEVRDKFPEVIIIFPDQQQ---KSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTKIDLP--------TENSNSEVIVITGKKSNCEAARERILAIEKELASMKEVEVSIPAKLHNSLI---GSKGSLVRSIMEDCGGVHIHFPAEGSGLDR---VTIRGPAEEVE-RARKQLLQLAEEKQVNNFSVELQAK--PEYHKYLIGRGGANIRRVRDRTGARIIFPSADDQEQEHITIMGKEEAVILAQRELETLIKNLDDVIEDSMI--------VEPRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGTHSDSITLKGPRECVDAAKKRIQEIVCDLESQVSMEVL-IPQRYHRAIMGPKGCRIQQITREHEVQIKFPDR 909 HSP 5 Score: 95.1301 bits (235), Expect = 4.796e-19 Identity = 118/532 (22.18%), Postives = 235/532 (44.17%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNT-ICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGK--------SGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRII---GQKENV 2215
I ++G D V +A I+ + Q AT + IPKE H+++IG G ++E+ +T+ + I PN I + G K+ + A + L+ + + + + H FI G S +S+ P+I ++IVI G E V ++ + + + + + +E+ K+I+G G +Q+I + T S+ P D + + + G P + + +++ + + H +IG++G+ + I + P+V I F D + + I L GP ++V+Q +Q+ L+ ++ I ++H+ +IG+ G I + + +IP SE + I G + V+ A K + ++ ++ N + I + H +IG+ G +I +++ V I FP S+ V ++G K++V K L L+ E+ S++ + + ++ + +KK+RE + +I + S+ +I G+K N
Sbjct: 124 IMVTGKLDSVMKARKEIVARLQTQASAT-----VLIPKEHHRFVIGKNGEKLQELELKTATKIAIP----RSDDPNANIRITGTKEGIEKARHEIQLISAEQDKRAVERLCFEKAFHPFIAGAYNRLVQELSQETGARISIPPPSI------PKDEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTGVSVEMPPLDSASETIILRGEPDKLGPALTQVYAKAKSVIVAEVIAPAWLHRF---IIGKKGQNISRITQQLPKVHIEF--TDGE-ERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERCGLVRIEGDPQGVQLARKELMDMAQRMENERTKDLIIEQKFHRTIIGQKGEKIKEVRDKF-PEVIIIFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEVIVITGKKSNC 633 HSP 6 Score: 54.299 bits (129), Expect = 1.287e-6 Identity = 116/516 (22.48%), Postives = 224/516 (43.41%), Query Frame = 2
Query: 827 EILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQLRFEDEANQ-IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVE-AVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAA----SEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXX-XXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASN-----YEQHFQMTLEDYEFLFMSADLKLKKLRETYS---VRISEAEDKSHL-RIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKS 2320
+I+ +T ++++ L + +I + G D++ A + + ++ + P H+F+IGK+G L +L + I + D+ N I I G+ +E IE + + + + Q + + + + I G+ +Q++ +ET I+ P + K + I G V A+ I E T + + ++G +G L+EI + V + PP D+ S+ I L R + D++ L + +A +S +V P H+ IIG++G I +T+ Q+P ++ + I + G ++VE A ++ I + ++ I + H LIGK G IN +KE +V++R P V I+G V A+ +L D+ + + EQ F T + K+K++R+ + + + + KS + ++ G K V + LQK +E+ V + ++S+ F K+
Sbjct: 103 DIMQKTGAHIELS---LAKDQGLSIMVTGKLDSVMKARKEIVARLQTQASATVLIPKEHHRFVIGKNGEKLQELELKTATKIAIPRSDDPNANIRITGT-KEGIEKARHEIQLISAEQDKRAVERLCFEKAFHPFIAGAYNRLVQELSQETGARISIPPPSIPKDEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTG-VSVEMPPLDSASETIIL---RGEPDKLGPALTQVYA-KAKSVIVAEVIAPAWLHRFIIGKKGQNISRITQ------QLPKVHIEFTDGEERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRFHRHLIGKNGANINRIKEQY--KVSVRIPQDSERCGLVRIEGDPQGVQLARKELMDMAQRMENERTKDLIIEQKFHRT------IIGQKGEKIKEVRDKFPEVIIIFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAEL---VESSFSISVPIHKQFHKN 592 HSP 7 Score: 53.1434 bits (126), Expect = 2.571e-6 Identity = 41/127 (32.28%), Postives = 67/127 (52.76%), Query Frame = 3
Query: 225 VTPDQ---SLGVFISGQKDKVCEAKKY----ITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LV 584
+ PDQ S V + G K++V + K+ I LV++ F IS+ I K+FH+++IG G+ K++ T+T++ P N S +I I G K E AR+ I E + + V I + + N L+
Sbjct: 540 IFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEVIVITGKKSNCEAARERILAIEKELASMKEVEVSIPAKLHNSLI 666
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Zebrafish
Match: hdlbpb (high density lipoprotein binding protein b [Source:ZFIN;Acc:ZDB-GENE-030131-6822]) HSP 1 Score: 359.762 bits (922), Expect = 1.226e-122 Identity = 279/1007 (27.71%), Postives = 519/1007 (51.54%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTK-KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTIS--NSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYK-----LEITGHKDKITLAKEEII-------------KLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIV-TGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS-NINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIH--SRLIGSRGANLD 3490
K +H N + + + R+ IPP S+ K+EI I+G+++ VA A + + TY + K + +E+ K +HKYI+GP+G+ ++EIL+ T V V++ L+ S TI L G D LGPALT V+ K++SV + + AP WLH+FIIGK G N+ ++ P + + F D +I +EG EEV + +++ + DL R+ E+ ID +H+IG G NI +I+++ S+ P+D + +V I G P GV+ + L+ + +EN + KD+I +IGQ+G+ +KE++D+FPEV I FP SD++ L GP+ +V++ A LQK A LVES+FS V I ++HK IIG+ G I+++ + TNTK +P + ++ IVITGK+ + E A +R+ I ++ +++ E+ + I LH LIG G+ + S+ E CGG V I FP+ + + V I+G ++V +A+ +L L EE +N+ Q E +++L ++++R+ RI ++ +++ H+ I+G++E V+ L+ + ++ +++ + +F + ++ R + + V ++ ++ + + +GP + + +AK I I+ D E+ ++ +++ +R + I ++ +I+++++VQI+ P E + S N P + + + + E+ + + +P I E + + +Y I + S I + +D NI +PQ + ++ITG + AK ++ L + ++V +++P +IG KG I ++R + V+++ P+K + VIV +G + AK I + V L+ M S + + + H ++IG +G+ I KI + V I P S + N++T+ G +NV+ + +L + + E + + + S+ +G+ G + D
Sbjct: 228 KAFHPFIAGAYNRLVQELSQETGARISIPPPSIPKDEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTGVSVEMPP--LDSAS-ETIILRGEPDKLGPALTQVYAKAKSVIVAEVIAPAWLHRFIIGKKGQNISRITQQLPKVHIEFTDGEERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERCGLVRIEGDPQGVQLARKELMDMAQRMENERTKDLIIEQKFHRTIIGQKGEKIKEVRDKFPEVIIIFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEVIVITGKKSNCEAARERILAIEKELASMKEVEVSIPAKLHNSLIGSKGSLVRSIMEDCGG-VHIHFPAEGSGLDRVTIRGPAEEVERARKQLLQLAEEKQVNNFSVELQAKPEYHKYLIGRGGANIRRVRDRTGARIIFPSADDQEQEHITIMGKEEAVILAQRELETLIKNLDDVIEDSMIVEPRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGTHSDS---ITLKGPRECVDAAKKRIQEIVCDLESQVSMEVLIPQRYHRAIMGPKGCRIQQITREHEVQIKFPDRDESAAQEPTSQENGDTDLTPRK---CDIITVTGRAEKCELARAALLALVPVTIDVEVSYDLHRY-----IIGQKGSGIR---KMMEDYEVNIWVPQPEQQSDIIKITGQVASVERAKHGLLDRVKELQAEQEDRALRSYKVTLSVDPKYHPKIIGRKGAVISQIRKEYDVNVQFPDKGDEQQDVIVISGYERNANDAKSAIEQLVAALQEM--------VSEDVRLDRRVHARIIGARGKAIRKIMEEFKVDIRFPQPGSEDPNKVTVTGLPENVDNTIDHLLNLEEEYMLSVTETETMAAYMKPPSKTMGTGGNDED 1208 HSP 2 Score: 102.834 bits (255), Expect = 1.226e-122 Identity = 54/139 (38.85%), Postives = 83/139 (59.71%), Query Frame = 3
Query: 102 FITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQ 509
IT F +P EERRY D+ + K+ +I G +E ++ DQ L + ++G+ D V +A+K I +QTQ ++ IPKE HR +IG G +ELE T+T+++ P+ +DP+ I+I G+K+GIE AR IQ
Sbjct: 72 VITQVFHVPLEERRYKDNSQFGEGEEAKVCLDIMQKTGAHIELSLAKDQGLSIMVTGKLDSVMKARKEIVARLQTQASATVLIPKEHHRFVIGKNGEKLQELELKTATKIAIPRSDDPNANIRITGTKEGIEKARHEIQ 210 HSP 3 Score: 181.03 bits (458), Expect = 1.806e-45 Identity = 264/1149 (22.98%), Postives = 502/1149 (43.69%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAA-SILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFT----KSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKP-NIQLRFEDEANQ-IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKE-TNCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCN-IYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRE-TYSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTY-FQKSNEL--FRIGRDNMC------KLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQ-VQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTI-------SAGVALNKDPISNIKLPQYKLEI---TGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTH---------KSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSW 3883
K +H+ K+ L + + + ++ IP I I+G ++G+ +A I L + Q K + L E K H +I G ++E+ +T + I + + + I + G K+ + A+ + K I+++ HK+I+G G+ L ++ N ++++ D A++ I++ G P+++ +V+ + E+ + + IIG G NI +I ++ I F D +++ T +A I + + +I LIG+ G + IK+++ +V + P L+ + G + V Q+A A +E+ +K + I K+H+ IIG++G I+E+ K P +++D + + G + +VE K + I E + I + I K H +IGKGG I ++E + I P+ +++S ++I G K + A+ ++ + +E+ AS E + + + L S ++ + E V I + S L + + E +++AR ++ + E +N+ + Q E + IGR + ++ EQ + G + + A+ E+ +I + ++ I +++IV+ R ++ + E++++Y V + P +S T+ +K V S+ + P IM G K I +I E I SA + L K +I TG +K LA+ ++ L + ++V V + + +IG KG I+K+ ++V+I +P + + +TG SV AK +++ V+EL+ +++R +R++ + L V +YH K+IG KG IS+IR + V + P + D + I I G++ N AK+ + V L+ + E + +D R+H+R+IG+RG + ++ +++KV+I FP G+ E+ N V V G E+VD D L++ + + L ++ + +P P+K+ T GF +R+APW+A GN SSA + G P QTS+W
Sbjct: 156 KEHHRFVIGKNGEKLQELELKTATKIAIPRSDDPNANIRITGTKEGIEKARHEIQLISAEQDKRAVERLCFE--KAFHPFIAGAYNRLVQELSQETGARISIPPPSIPK---DEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTGVSVEMPPLDSASETIILRGEPDKLGPAL----TQVYAKAKSVIVAEVIAPAWLHRFIIGKKGQNISRITQQLPKVHIEFT-DGEERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRFHRHLIGKNGANINRIKEQY-KVSVRIPQDSERCGLVRIEGDPQGV-QLARKELMDMAQRMENERTKDLIIEQKFHRTIIGQKGEKIKEVRDKFPEVIIIFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTK--IDLPTENSNSEVIVITGKKSNCEAARERILAIEKEL-ASMKEVEVSIPAKLHNSLIGSKGSLVRSIMEDCGGVHIHFPAEGSGL----DRVTIRGPAEEVERARKQLLQLAEEK---QVNNFSVELQAKPEYHKYLIGRGGANIRRVRDRTGARIIFPSADDQEQEHITIMGKEEAVILAQRELETLIKNLDDVIEDSMIVEPRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGTHSDSITLKGPRECVDAAKKRIQEIVCDLESQVSMEVLI---PQRYHRAIM------GPKGCRIQQITREHEVQIKFPDRDESAAQEPTSQENGDTDLTPRKCDIITVTGRAEKCELARAALLALVPVTIDVEVSYDLHRYIIGQKGSGIRKMMEDYEVNIWVPQPEQQSDIIKITGQVASVERAKHGLLDRVKELQAEQEDRALRSYKVTLSVDPKYHPKIIGRKGAVISQIRKEYDVNVQFPDKGDEQQDVIVISGYERNANDAKSAIEQLVAALQEMVSEDVRLDRRVHARIIGARGKAIRKIMEEFKVDIRFPQPGS--EDPNKVTVTGLPENVDNTIDHLLN--------LEEEYMLSVTETETMAAYMKP-----PSKTMGTGGNDEDNRALAKGFVVRDAPWNAQGNKAPDVSSAEEFPTFGSG----ITPKQTSAW 1254 HSP 4 Score: 112.849 bits (281), Expect = 1.627e-24 Identity = 189/869 (21.75%), Postives = 383/869 (44.07%), Query Frame = 2
Query: 1013 IIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEI------TASNYEQHFQMTLEDYEFLFMSADLKLKKLRETY--SVRISEAEDKSHL-RIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK--------SNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDY-ENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPN-----PNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISA-GVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTK-VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGI-IEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIR 3535
I+ K+G++ ++LS+ K D+ I++ G + V++ K+ V LQ++ S + I + +IG G +Q++ +T I PR D + I GT G+E + + + + + F P + G + ++E+ RI+ PPP D I + G ++ V A ++ + + V++ HK I+G +G T++E+ + T ++P ++ I++ G+ + A+ +V Y K ++I + LH +IGK G I+ + + +V I F + + ++G ++V +AQ+++ ++++++ T N +Q F + L + +++E Y SVRI + ++ L RI G + V Q AR E+ + DL QK E + RD ++ + + + I++ RGP ++ + +I + E++ + ++ + ++ KNI + +I ++ +I +P SNS V G+K E + P L N ++ S GS +I E V A G L++ I+ P ++E K + LA+E+ + F ++L+ E++ LIG G I+++R I P+ + + + + + G E+V+ A++E+ ++ L+ + ++ I V ++HR + +G+ + ++ + V +S P ++ + IT++G ++ V+ AK + V LES + +EVL I R H ++G +G + Q+ ++++V+I FP R
Sbjct: 104 IMQKTGAH-IELSLAK--------DQGLSIMVTGKLDSVMKAR---KEIVARLQTQASATVL-IPKEHHRFVIGKNGEKLQELELKTATKIAIPRSDDPNANIRITGTKEGIEKARHEIQLISAEQDKRAVERLCFEKAFHPFIAGAYNRLVQELSQETG-ARISIPPPSIPKDEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTGVSVEMPPLDSASETIILRGEPDKLGPALTQV---YAKAKSVIVAEVIAPAWLHRFIIGKKGQNISRITQQLP-KVHIEFTDGE---ERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRF------HRHLIGKNGANINRIKEQYKVSVRIPQDSERCGLVRIEGDPQGV-------QLARKELMDMAQRMENERTKDLIIEQKFHRTIIGQKGEKIKEVRDKFPEVIIIFPDQQQ---KSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTKIDLP--------TENSNSEVIVITGKKSNCEAARERILAIEKELASMKEVEVSIPAKLHNSLI---GSKGSLVRSIMEDCGGVHIHFPAEGSGLDR---VTIRGPAEEVE-RARKQLLQLAEEKQVNNFSVELQAK--PEYHKYLIGRGGANIRRVRDRTGARIIFPSADDQEQEHITIMGKEEAVILAQRELETLIKNLDDVIEDSMI--------VEPRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGTHSDSITLKGPRECVDAAKKRIQEIVCDLESQVSMEVL-IPQRYHRAIMGPKGCRIQQITREHEVQIKFPDR 909 HSP 5 Score: 95.1301 bits (235), Expect = 4.796e-19 Identity = 118/532 (22.18%), Postives = 235/532 (44.17%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNT-ICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGK--------SGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRII---GQKENV 2215
I ++G D V +A I+ + Q AT + IPKE H+++IG G ++E+ +T+ + I PN I + G K+ + A + L+ + + + + H FI G S +S+ P+I ++IVI G E V ++ + + + + + +E+ K+I+G G +Q+I + T S+ P D + + + G P + + +++ + + H +IG++G+ + I + P+V I F D + + I L GP ++V+Q +Q+ L+ ++ I ++H+ +IG+ G I + + +IP SE + I G + V+ A K + ++ ++ N + I + H +IG+ G +I +++ V I FP S+ V ++G K++V K L L+ E+ S++ + + ++ + +KK+RE + +I + S+ +I G+K N
Sbjct: 124 IMVTGKLDSVMKARKEIVARLQTQASAT-----VLIPKEHHRFVIGKNGEKLQELELKTATKIAIP----RSDDPNANIRITGTKEGIEKARHEIQLISAEQDKRAVERLCFEKAFHPFIAGAYNRLVQELSQETGARISIPPPSI------PKDEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTGVSVEMPPLDSASETIILRGEPDKLGPALTQVYAKAKSVIVAEVIAPAWLHRF---IIGKKGQNISRITQQLPKVHIEF--TDGE-ERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERCGLVRIEGDPQGVQLARKELMDMAQRMENERTKDLIIEQKFHRTIIGQKGEKIKEVRDKF-PEVIIIFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEVIVITGKKSNC 633 HSP 6 Score: 54.299 bits (129), Expect = 1.287e-6 Identity = 116/516 (22.48%), Postives = 224/516 (43.41%), Query Frame = 2
Query: 827 EILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQLRFEDEANQ-IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVE-AVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAA----SEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXX-XXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASN-----YEQHFQMTLEDYEFLFMSADLKLKKLRETYS---VRISEAEDKSHL-RIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKS 2320
+I+ +T ++++ L + +I + G D++ A + + ++ + P H+F+IGK+G L +L + I + D+ N I I G+ +E IE + + + + Q + + + + I G+ +Q++ +ET I+ P + K + I G V A+ I E T + + ++G +G L+EI + V + PP D+ S+ I L R + D++ L + +A +S +V P H+ IIG++G I +T+ Q+P ++ + I + G ++VE A ++ I + ++ I + H LIGK G IN +KE +V++R P V I+G V A+ +L D+ + + EQ F T + K+K++R+ + + + + KS + ++ G K V + LQK +E+ V + ++S+ F K+
Sbjct: 103 DIMQKTGAHIELS---LAKDQGLSIMVTGKLDSVMKARKEIVARLQTQASATVLIPKEHHRFVIGKNGEKLQELELKTATKIAIPRSDDPNANIRITGT-KEGIEKARHEIQLISAEQDKRAVERLCFEKAFHPFIAGAYNRLVQELSQETGARISIPPPSIPKDEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTG-VSVEMPPLDSASETIIL---RGEPDKLGPALTQVYA-KAKSVIVAEVIAPAWLHRFIIGKKGQNISRITQ------QLPKVHIEFTDGEERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRFHRHLIGKNGANINRIKEQY--KVSVRIPQDSERCGLVRIEGDPQGVQLARKELMDMAQRMENERTKDLIIEQKFHRT------IIGQKGEKIKEVRDKFPEVIIIFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAEL---VESSFSISVPIHKQFHKN 592 HSP 7 Score: 53.1434 bits (126), Expect = 2.571e-6 Identity = 41/127 (32.28%), Postives = 67/127 (52.76%), Query Frame = 3
Query: 225 VTPDQ---SLGVFISGQKDKVCEAKKY----ITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LV 584
+ PDQ S V + G K++V + K+ I LV++ F IS+ I K+FH+++IG G+ K++ T+T++ P N S +I I G K E AR+ I E + + V I + + N L+
Sbjct: 540 IFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEVIVITGKKSNCEAARERILAIEKELASMKEVEVSIPAKLHNSLI 666
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Zebrafish
Match: hdlbpb (high density lipoprotein binding protein b [Source:ZFIN;Acc:ZDB-GENE-030131-6822]) HSP 1 Score: 359.762 bits (922), Expect = 1.226e-122 Identity = 279/1007 (27.71%), Postives = 519/1007 (51.54%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTK-KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTIS--NSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYK-----LEITGHKDKITLAKEEII-------------KLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIV-TGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS-NINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIH--SRLIGSRGANLD 3490
K +H N + + + R+ IPP S+ K+EI I+G+++ VA A + + TY + K + +E+ K +HKYI+GP+G+ ++EIL+ T V V++ L+ S TI L G D LGPALT V+ K++SV + + AP WLH+FIIGK G N+ ++ P + + F D +I +EG EEV + +++ + DL R+ E+ ID +H+IG G NI +I+++ S+ P+D + +V I G P GV+ + L+ + +EN + KD+I +IGQ+G+ +KE++D+FPEV I FP SD++ L GP+ +V++ A LQK A LVES+FS V I ++HK IIG+ G I+++ + TNTK +P + ++ IVITGK+ + E A +R+ I ++ +++ E+ + I LH LIG G+ + S+ E CGG V I FP+ + + V I+G ++V +A+ +L L EE +N+ Q E +++L ++++R+ RI ++ +++ H+ I+G++E V+ L+ + ++ +++ + +F + ++ R + + V ++ ++ + + +GP + + +AK I I+ D E+ ++ +++ +R + I ++ +I+++++VQI+ P E + S N P + + + + E+ + + +P I E + + +Y I + S I + +D NI +PQ + ++ITG + AK ++ L + ++V +++P +IG KG I ++R + V+++ P+K + VIV +G + AK I + V L+ M S + + + H ++IG +G+ I KI + V I P S + N++T+ G +NV+ + +L + + E + + + S+ +G+ G + D
Sbjct: 228 KAFHPFIAGAYNRLVQELSQETGARISIPPPSIPKDEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTGVSVEMPP--LDSAS-ETIILRGEPDKLGPALTQVYAKAKSVIVAEVIAPAWLHRFIIGKKGQNISRITQQLPKVHIEFTDGEERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERCGLVRIEGDPQGVQLARKELMDMAQRMENERTKDLIIEQKFHRTIIGQKGEKIKEVRDKFPEVIIIFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEVIVITGKKSNCEAARERILAIEKELASMKEVEVSIPAKLHNSLIGSKGSLVRSIMEDCGG-VHIHFPAEGSGLDRVTIRGPAEEVERARKQLLQLAEEKQVNNFSVELQAKPEYHKYLIGRGGANIRRVRDRTGARIIFPSADDQEQEHITIMGKEEAVILAQRELETLIKNLDDVIEDSMIVEPRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGTHSDS---ITLKGPRECVDAAKKRIQEIVCDLESQVSMEVLIPQRYHRAIMGPKGCRIQQITREHEVQIKFPDRDESAAQEPTSQENGDTDLTPRK---CDIITVTGRAEKCELARAALLALVPVTIDVEVSYDLHRY-----IIGQKGSGIR---KMMEDYEVNIWVPQPEQQSDIIKITGQVASVERAKHGLLDRVKELQAEQEDRALRSYKVTLSVDPKYHPKIIGRKGAVISQIRKEYDVNVQFPDKGDEQQDVIVISGYERNANDAKSAIEQLVAALQEM--------VSEDVRLDRRVHARIIGARGKAIRKIMEEFKVDIRFPQPGSEDPNKVTVTGLPENVDNTIDHLLNLEEEYMLSVTETETMAAYMKPPSKTMGTGGNDED 1208 HSP 2 Score: 102.834 bits (255), Expect = 1.226e-122 Identity = 54/139 (38.85%), Postives = 83/139 (59.71%), Query Frame = 3
Query: 102 FITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQ 509
IT F +P EERRY D+ + K+ +I G +E ++ DQ L + ++G+ D V +A+K I +QTQ ++ IPKE HR +IG G +ELE T+T+++ P+ +DP+ I+I G+K+GIE AR IQ
Sbjct: 72 VITQVFHVPLEERRYKDNSQFGEGEEAKVCLDIMQKTGAHIELSLAKDQGLSIMVTGKLDSVMKARKEIVARLQTQASATVLIPKEHHRFVIGKNGEKLQELELKTATKIAIPRSDDPNANIRITGTKEGIEKARHEIQ 210 HSP 3 Score: 181.03 bits (458), Expect = 1.806e-45 Identity = 264/1149 (22.98%), Postives = 502/1149 (43.69%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAA-SILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFT----KSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKP-NIQLRFEDEANQ-IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKE-TNCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCN-IYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRE-TYSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTY-FQKSNEL--FRIGRDNMC------KLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQ-VQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTI-------SAGVALNKDPISNIKLPQYKLEI---TGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTH---------KSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSW 3883
K +H+ K+ L + + + ++ IP I I+G ++G+ +A I L + Q K + L E K H +I G ++E+ +T + I + + + I + G K+ + A+ + K I+++ HK+I+G G+ L ++ N ++++ D A++ I++ G P+++ +V+ + E+ + + IIG G NI +I ++ I F D +++ T +A I + + +I LIG+ G + IK+++ +V + P L+ + G + V Q+A A +E+ +K + I K+H+ IIG++G I+E+ K P +++D + + G + +VE K + I E + I + I K H +IGKGG I ++E + I P+ +++S ++I G K + A+ ++ + +E+ AS E + + + L S ++ + E V I + S L + + E +++AR ++ + E +N+ + Q E + IGR + ++ EQ + G + + A+ E+ +I + ++ I +++IV+ R ++ + E++++Y V + P +S T+ +K V S+ + P IM G K I +I E I SA + L K +I TG +K LA+ ++ L + ++V V + + +IG KG I+K+ ++V+I +P + + +TG SV AK +++ V+EL+ +++R +R++ + L V +YH K+IG KG IS+IR + V + P + D + I I G++ N AK+ + V L+ + E + +D R+H+R+IG+RG + ++ +++KV+I FP G+ E+ N V V G E+VD D L++ + + L ++ + +P P+K+ T GF +R+APW+A GN SSA + G P QTS+W
Sbjct: 156 KEHHRFVIGKNGEKLQELELKTATKIAIPRSDDPNANIRITGTKEGIEKARHEIQLISAEQDKRAVERLCFE--KAFHPFIAGAYNRLVQELSQETGARISIPPPSIPK---DEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTGVSVEMPPLDSASETIILRGEPDKLGPAL----TQVYAKAKSVIVAEVIAPAWLHRFIIGKKGQNISRITQQLPKVHIEFT-DGEERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRFHRHLIGKNGANINRIKEQY-KVSVRIPQDSERCGLVRIEGDPQGV-QLARKELMDMAQRMENERTKDLIIEQKFHRTIIGQKGEKIKEVRDKFPEVIIIFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTK--IDLPTENSNSEVIVITGKKSNCEAARERILAIEKEL-ASMKEVEVSIPAKLHNSLIGSKGSLVRSIMEDCGGVHIHFPAEGSGL----DRVTIRGPAEEVERARKQLLQLAEEK---QVNNFSVELQAKPEYHKYLIGRGGANIRRVRDRTGARIIFPSADDQEQEHITIMGKEEAVILAQRELETLIKNLDDVIEDSMIVEPRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGTHSDSITLKGPRECVDAAKKRIQEIVCDLESQVSMEVLI---PQRYHRAIM------GPKGCRIQQITREHEVQIKFPDRDESAAQEPTSQENGDTDLTPRKCDIITVTGRAEKCELARAALLALVPVTIDVEVSYDLHRYIIGQKGSGIRKMMEDYEVNIWVPQPEQQSDIIKITGQVASVERAKHGLLDRVKELQAEQEDRALRSYKVTLSVDPKYHPKIIGRKGAVISQIRKEYDVNVQFPDKGDEQQDVIVISGYERNANDAKSAIEQLVAALQEMVSEDVRLDRRVHARIIGARGKAIRKIMEEFKVDIRFPQPGS--EDPNKVTVTGLPENVDNTIDHLLN--------LEEEYMLSVTETETMAAYMKP-----PSKTMGTGGNDEDNRALAKGFVVRDAPWNAQGNKAPDVSSAEEFPTFGSG----ITPKQTSAW 1254 HSP 4 Score: 112.849 bits (281), Expect = 1.627e-24 Identity = 189/869 (21.75%), Postives = 383/869 (44.07%), Query Frame = 2
Query: 1013 IIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEI------TASNYEQHFQMTLEDYEFLFMSADLKLKKLRETY--SVRISEAEDKSHL-RIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK--------SNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDY-ENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPN-----PNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISA-GVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTK-VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGI-IEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIR 3535
I+ K+G++ ++LS+ K D+ I++ G + V++ K+ V LQ++ S + I + +IG G +Q++ +T I PR D + I GT G+E + + + + + F P + G + ++E+ RI+ PPP D I + G ++ V A ++ + + V++ HK I+G +G T++E+ + T ++P ++ I++ G+ + A+ +V Y K ++I + LH +IGK G I+ + + +V I F + + ++G ++V +AQ+++ ++++++ T N +Q F + L + +++E Y SVRI + ++ L RI G + V Q AR E+ + DL QK E + RD ++ + + + I++ RGP ++ + +I + E++ + ++ + ++ KNI + +I ++ +I +P SNS V G+K E + P L N ++ S GS +I E V A G L++ I+ P ++E K + LA+E+ + F ++L+ E++ LIG G I+++R I P+ + + + + + G E+V+ A++E+ ++ L+ + ++ I V ++HR + +G+ + ++ + V +S P ++ + IT++G ++ V+ AK + V LES + +EVL I R H ++G +G + Q+ ++++V+I FP R
Sbjct: 104 IMQKTGAH-IELSLAK--------DQGLSIMVTGKLDSVMKAR---KEIVARLQTQASATVL-IPKEHHRFVIGKNGEKLQELELKTATKIAIPRSDDPNANIRITGTKEGIEKARHEIQLISAEQDKRAVERLCFEKAFHPFIAGAYNRLVQELSQETG-ARISIPPPSIPKDEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTGVSVEMPPLDSASETIILRGEPDKLGPALTQV---YAKAKSVIVAEVIAPAWLHRFIIGKKGQNISRITQQLP-KVHIEFTDGE---ERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRF------HRHLIGKNGANINRIKEQYKVSVRIPQDSERCGLVRIEGDPQGV-------QLARKELMDMAQRMENERTKDLIIEQKFHRTIIGQKGEKIKEVRDKFPEVIIIFPDQQQ---KSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTKIDLP--------TENSNSEVIVITGKKSNCEAARERILAIEKELASMKEVEVSIPAKLHNSLI---GSKGSLVRSIMEDCGGVHIHFPAEGSGLDR---VTIRGPAEEVE-RARKQLLQLAEEKQVNNFSVELQAK--PEYHKYLIGRGGANIRRVRDRTGARIIFPSADDQEQEHITIMGKEEAVILAQRELETLIKNLDDVIEDSMI--------VEPRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGTHSDSITLKGPRECVDAAKKRIQEIVCDLESQVSMEVL-IPQRYHRAIMGPKGCRIQQITREHEVQIKFPDR 909 HSP 5 Score: 95.1301 bits (235), Expect = 4.796e-19 Identity = 118/532 (22.18%), Postives = 235/532 (44.17%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNT-ICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGK--------SGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRII---GQKENV 2215
I ++G D V +A I+ + Q AT + IPKE H+++IG G ++E+ +T+ + I PN I + G K+ + A + L+ + + + + H FI G S +S+ P+I ++IVI G E V ++ + + + + + +E+ K+I+G G +Q+I + T S+ P D + + + G P + + +++ + + H +IG++G+ + I + P+V I F D + + I L GP ++V+Q +Q+ L+ ++ I ++H+ +IG+ G I + + +IP SE + I G + V+ A K + ++ ++ N + I + H +IG+ G +I +++ V I FP S+ V ++G K++V K L L+ E+ S++ + + ++ + +KK+RE + +I + S+ +I G+K N
Sbjct: 124 IMVTGKLDSVMKARKEIVARLQTQASAT-----VLIPKEHHRFVIGKNGEKLQELELKTATKIAIP----RSDDPNANIRITGTKEGIEKARHEIQLISAEQDKRAVERLCFEKAFHPFIAGAYNRLVQELSQETGARISIPPPSI------PKDEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTGVSVEMPPLDSASETIILRGEPDKLGPALTQVYAKAKSVIVAEVIAPAWLHRF---IIGKKGQNISRITQQLPKVHIEF--TDGE-ERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERCGLVRIEGDPQGVQLARKELMDMAQRMENERTKDLIIEQKFHRTIIGQKGEKIKEVRDKF-PEVIIIFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEVIVITGKKSNC 633 HSP 6 Score: 54.299 bits (129), Expect = 1.287e-6 Identity = 116/516 (22.48%), Postives = 224/516 (43.41%), Query Frame = 2
Query: 827 EILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQLRFEDEANQ-IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVE-AVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAA----SEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXX-XXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASN-----YEQHFQMTLEDYEFLFMSADLKLKKLRETYS---VRISEAEDKSHL-RIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKS 2320
+I+ +T ++++ L + +I + G D++ A + + ++ + P H+F+IGK+G L +L + I + D+ N I I G+ +E IE + + + + Q + + + + I G+ +Q++ +ET I+ P + K + I G V A+ I E T + + ++G +G L+EI + V + PP D+ S+ I L R + D++ L + +A +S +V P H+ IIG++G I +T+ Q+P ++ + I + G ++VE A ++ I + ++ I + H LIGK G IN +KE +V++R P V I+G V A+ +L D+ + + EQ F T + K+K++R+ + + + + KS + ++ G K V + LQK +E+ V + ++S+ F K+
Sbjct: 103 DIMQKTGAHIELS---LAKDQGLSIMVTGKLDSVMKARKEIVARLQTQASATVLIPKEHHRFVIGKNGEKLQELELKTATKIAIPRSDDPNANIRITGT-KEGIEKARHEIQLISAEQDKRAVERLCFEKAFHPFIAGAYNRLVQELSQETGARISIPPPSIPKDEIVITGEKEAVAMAIARIRATYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEILENTG-VSVEMPPLDSASETIIL---RGEPDKLGPALTQVYA-KAKSVIVAEVIAPAWLHRFIIGKKGQNISRITQ------QLPKVHIEFTDGEERICLEGPTEEVEQAQIQIQEIIKDLMVRMDYTEINIDQRFHRHLIGKNGANINRIKEQY--KVSVRIPQDSERCGLVRIEGDPQGVQLARKELMDMAQRMENERTKDLIIEQKFHRT------IIGQKGEKIKEVRDKFPEVIIIFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAEL---VESSFSISVPIHKQFHKN 592 HSP 7 Score: 53.1434 bits (126), Expect = 2.571e-6 Identity = 41/127 (32.28%), Postives = 67/127 (52.76%), Query Frame = 3
Query: 225 VTPDQ---SLGVFISGQKDKVCEAKKY----ITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LV 584
+ PDQ S V + G K++V + K+ I LV++ F IS+ I K+FH+++IG G+ K++ T+T++ P N S +I I G K E AR+ I E + + V I + + N L+
Sbjct: 540 IFPDQQQKSDIVQLRGPKNEVEKCAKFLQKLIAELVESSFSISVPIHKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEVIVITGKKSNCEAARERILAIEKELASMKEVEVSIPAKLHNSLI 666
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Zebrafish
Match: hdlbpa (high density lipoprotein binding protein a [Source:ZFIN;Acc:ZDB-GENE-030131-2032]) HSP 1 Score: 128.257 bits (321), Expect = 6.510e-53 Identity = 64/190 (33.68%), Postives = 110/190 (57.89%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEV 1129
K +H + +++ R+++PP SV+K EI I+G+++ VA A + + Y + K + +E+ K +HKY+IGP+G+ ++EIL++T V V+I + S T+ L G LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG ++V
Sbjct: 234 KVFHPFITGAHGKLVGDLMQETGARINVPPPSVNKTEIVITGEKEQVALAMVKIKKLYEEKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILERTGVSVEIPPL---DSSSETVILRGEPARLGQALTEVYAKANSYTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTKDV 420 HSP 2 Score: 101.679 bits (252), Expect = 6.510e-53 Identity = 55/139 (39.57%), Postives = 82/139 (58.99%), Query Frame = 3
Query: 102 FITDTFVIPYEERRYID----SESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
IT F +P EER+Y D E ++ K L +I G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G +ELE T+T++ P+ +DPS IKI G+K+G+E A+ I
Sbjct: 78 IITQVFHVPLEERKYKDMNQFGEGDQAKVCL-DIMHKTGAHLELSMAKDQGLSIMVSGKYDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKSGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEI 215 HSP 3 Score: 72.0182 bits (175), Expect = 2.223e-12 Identity = 41/144 (28.47%), Postives = 75/144 (52.08%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFP 3529
++V+G ++V+ A+KEIV ++ S + +P ++HR +IG GE + ++ K + +I +P D N+I I G K+ +EKAK+E+L + + +E +NID H + G+ G + L ++ I P
Sbjct: 130 IMVSGKYDAVMKARKEIVSRLQ-----------TQASATVAIPKEHHRFVIGKSGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEILLISAEQDKRAVERMNIDKVFHPFITGAHGKLVGDLMQETGARINVP 262 HSP 4 Score: 70.8626 bits (172), Expect = 4.695e-12 Identity = 79/311 (25.40%), Postives = 139/311 (44.69%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDL--------SVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDV 1564
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++E+ +T+ + I R ++ S N I + G K+ L A + L+ + + + ++ H FI G G + DL +V P++ +IVI G E+V +KK + + + + +E+ K++IG G +Q+I + T S+ P D + + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP KDV
Sbjct: 130 IMVSGKYDAVMKARKEIVSRLQTQASAT-----VAIPKEHHRFVIGKSGEKLQELELKTATKIQIP--RPDDPS-NQIKISGTKEGLEKAKHEILLISAEQDKRAVERMNIDKVFHPFITGAHGKLVGDLMQETGARINVPPPSVN------KTEIVITGEKEQVALAMVKIKKLYEEKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILERTGVSVEIPPLDSSSETVILRGEPARLGQALTEVYAKANSYTVSSVSAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTKDV 420 HSP 5 Score: 65.4698 bits (158), Expect = 2.467e-10 Identity = 92/355 (25.92%), Postives = 160/355 (45.07%), Query Frame = 2
Query: 773 IPKEKHKYI-IGPRGSGIKEILDQTSVWVDIN-------EQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQL-RFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVE-AVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAA----SEENDEIVITGKQKDVE 1786
+P E+ KY + G G DQ V +DI E + + +I + G D + A + ++ ++ + P H+F+IGKSG L +L + IQ+ R +D +NQI I G+ +E +E +++ + Q + + M ID + I G+ G + + +ET IN P + K + I G V A+ I E +N + + +IG +G L+EI +R V + PP D+ S+ + L G P + + + K +++ V S V P H+ IIG++G + ++T+ Q+P +E D+I + G KDV+
Sbjct: 85 VPLEERKYKDMNQFGEG-----DQAKVCLDIMHKTGAHLELSMAKDQGLSIMVSGKYDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKSGEKLQELELKTATKIQIPRPDDPSNQIKISGT-KEGLEKAKHEILLISAEQDKRAVERMNIDKVFHPFITGAHGKLVGDLMQETGARINVPPPSVNKTEIVITGEKEQVALAMVKIKKLYEEKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILERT-GVSVEIPPLDSSSETVILRGEPARLGQALTEVYAKANSYTVSS-----VSAPSWLHRFIIGKKGQNLAKITQ------QMPKVHIEFTEGEDKITLEGPTKDVQ 421 HSP 6 Score: 65.0846 bits (157), Expect = 3.151e-10 Identity = 55/245 (22.45%), Postives = 111/245 (45.31%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLE--VNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLA-FVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRAN 3628
++G D + A++EI+ V +P+E + +IG GEK+++L I+IP + ++ ++G+ E + AK EI+ E ++ ER +++ +H + G G+ + + + RI+VP N EI I G K+ V A ++ + K ++ + + H +IG +G L ++ ++ V + P + E V ++G + + E+ +AN
Sbjct: 132 VSGKYDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKSGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEILLISAEQDKRAVER--------MNIDKVFHPFITGAHGKLVGDLMQETGARINVPPPSVNKTEIVITGEKEQVALAMVKIKKLYEEKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILERTGVSVEIPPLDSSSE---TVILRGEPARLGQALTEVYAKAN 365 HSP 7 Score: 62.3882 bits (150), Expect = 2.168e-9 Identity = 71/313 (22.68%), Postives = 139/313 (44.41%), Query Frame = 2
Query: 1013 IIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRF 1948
I+ K+G++L +LS+ K D+ I++ G + V++ K+ V LQ++ S + I + +IG G +Q++ +T I PR D + I GT G+E K +L + + + P + G GK + ++ RIN PPP + I + G ++ V ++K + ++ + V++ HK +IG +G T++E+ + T +IP ++ +++ G+ + A+ +Y K ++ + LH +IGK G + + + +V I F
Sbjct: 110 IMHKTGAHL-ELSMAK--------DQGLSIMVSGKYDAVMKAR---KEIVSRLQTQASAT-VAIPKEHHRFVIGKSGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEILLISAEQDKRAVERMNIDKVFHPFITGAHGKLVGDLMQETG-ARINVPPPSVNKTEIVITGEKEQVALAMVKIKKLYEEKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILERTGVSVEIPPLDSSSETVILRGEPARLGQAL---TEVYAKANSYTVSSVSAPSWLHRFIIGKKGQNLAKITQQM-PKVHIEF 404 HSP 8 Score: 55.4546 bits (132), Expect = 3.154e-7 Identity = 58/252 (23.02%), Postives = 114/252 (45.24%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAA-SILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALT----LVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDL------SVNKPNIQLRFEDEANQIVIEGSP----EEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKE 1270
K +H+ KS L + + + ++ IP N+I ISG ++G+ +A ILL + Q K + + I+ K H +I G G + +++ +T +++ +N+ I + G K+ + A+ L K ++ IA++ HK++IG G+ L ++ SV P + + + +++ G P + + EV Y K + + S + + + IIG G N+ KI ++
Sbjct: 162 KEHHRFVIGKSGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEILLISAEQDKRAVERMNID--KVFHPFITGAHGKLVGDLMQETGARINVPPPSVNK---TEIVITGEKEQVALAMVKIKKLYEEKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILERTGVSVEIPPL----DSSSETVILRGEPARLGQALTEV--YAKANSYTVSS------VSAPSWLHRFIIGKKGQNLAKITQQ 396
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Xenopus
Match: hdlbp (high density lipoprotein binding protein (vigilin) [Source:NCBI gene;Acc:448610]) HSP 1 Score: 347.436 bits (890), Expect = 4.269e-117 Identity = 289/1011 (28.59%), Postives = 522/1011 (51.63%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDP------LMIKHIIG-SGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIG---RDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP---FYP--EP-VNSKTISNSHVSAKPGEKPYMNGV-------DXXXXXXXXXXXXXP------NPNHLPNDIMSEHNSNGSKYHNISEINSEVSS------TIS-AGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFT-KVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS-NINEITIQGHKDNVEKAKNEVLAFVNKLESGII--EVLNIDSRIHS 3457
K YH N + +++ + R++IPP SV+K EI +G+++ +AQA + + Q Y + K + +E+ K +HKY+IGP+G+ ++EIL++T V V+I ++ S T+ L G + LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V + ++ V DL + + + + L+++ +IG + + KI+++ S+ P+D K + I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+++FPEV INFP P SD++ L GP+ +V++ LQK + LVE+++S V I ++HK IIG+ G I+++ + NTK +PA + ++ IVITGK+ + E A R+ I ++ +NI E+ + I LH LIG G I S+ E CGG V I FPS + S+TV I+G DV +A+ +L L EE ++ + E ++FL ++K+R+ RI ++ +D+ + I+G +++V + L+ + IV +++++ +F + R G RD + + + + +G + +++AK +I+ II+D E +T ++ +R ++I ++ +I++++ VQI+ P P EP V + V A E P + + + ++ K+L P P L I+ + S K + E+N +V S TI+ G + N D L + + E+ ++ + ++ F L + V+ +F+P +IG KG I +R+ H V+I+ P+KN + ++I+TG + +A+ I+ V +LE+M E + V + H ++IG +G+ I KI + V I P + + N +T+ G ++V++A + +L + S ++ EV+ + HS
Sbjct: 228 KAYHPFIAGPYNKVVTEIMQETATRINIPPPSVNKTEIVCTGEKEQLAQAVARIKQIYEEKKKKTTSIAVEVRKSQHKYVIGPKGNNLQEILERTGVSVEIPP---SDSSSETVILRGEPEKLGQALTEVYAKANSFTVASVSAPSWLHRFIIGKKGQNLAKITQQIPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALVRDLVKYFTCICVYVIHVYVLFLLVMRSVIGLMDHIAVNKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANITELEVNIPSKLHNSLIGAKGRFIRSIMEECGG-VHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEEKQTKSFTSELKAKPEYHKFLIGKGGGNIRKVRDQTGARIIFPTADDKDQQLITIVGTEDSVKEAQKELEFLIKSLDNIVEDSMSVDPKHHRHF-----VIRRGQVLRDIAEEYGGVIVSFPRSGVQSDRVTLKGAKECVEAAKKKILDIIEDLEAQVTIECLITQRFHRSIMGPKGIRVQQITREFGVQIKFPDREVTPQGEPHVQENGDEGTEVKATDPESPKKCDIILISGRKEKCEAASDALKALVPVSIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSPEQQCDTITITGQSANLDRAKAGLLERVR-ELQAEQE------DRALRSFKLAVSVD--PKFHPKIIGRKGAVISLIRSDHDVNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSE--------DITVDHRVHARIIGARGKAIRKIMDEFKVDIRFPQTGAPDPNLVTVTGKPEDVDEAIDHLLNLEEEYMSDVVDNEVMQSYMKPHS 1212 HSP 2 Score: 97.4413 bits (241), Expect = 4.269e-117 Identity = 53/138 (38.41%), Postives = 79/138 (57.25%), Query Frame = 3
Query: 102 FITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
IT F +P EER+Y D + K+ +I G ++E ++ DQ L + +SG+ D V +A+K I +QTQ S+ IPKE HR +IG G ++LE T+T++ P+ + S IKI G+K+GIE AR I
Sbjct: 72 IITQVFHVPLEERKYKDMAQFGEGEQAKICLDIMQKTGAQLELSLAKDQGLSIMVSGKHDSVMKARKEIVTRLQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGTKEGIEKARHEI 209 HSP 3 Score: 172.555 bits (436), Expect = 1.169e-42 Identity = 105/330 (31.82%), Postives = 179/330 (54.24%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEI--NDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSW 3883
I+G K+K A + + L + +EV VP + + +IG KG I+K+ +V+I++P+ + + +TG S ++ AK ++E V EL+ +++R +R+F LA+ V ++H K+IG KG IS IR+ + V I P + D N ++I I G++ N E A++ +L V LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N+V V G E VD D L++ + ++ N+ MQ S+ ES QP + GF +R+APW N+ S+S+ + G P+ +TS W
Sbjct: 952 ISGRKEKCEAASDALKALVPVSIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSPEQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHPKIIGRKGAVISLIRSDHDVNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSEDITVDHRVHARIIGARGKAIRKIMDEFKVDIRFPQTG--APDPNLVTVTGKPEDVDEAIDHLLNLEEEYMSDVVDNEVMQSYMKPHSHEESKSQPSK-------------GFVVRDAPWTTGSNEKAPDMSSSEDFPIFG----TPVAHKTSPW 1262 HSP 4 Score: 116.316 bits (290), Expect = 2.332e-25 Identity = 155/696 (22.27%), Postives = 300/696 (43.10%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXE-QGILEFRGPVQNIKSAKDEIIHIIDD---YENTITENLI-------VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSST-ISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFV---LDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES-GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
+++ S V IP ++H+ +IG+ G +++L T TK QIP E +++I ITG ++ +E A + I ++ +EI K H + G + + + R+ I P P + ++ G K+ + +A ++ + EE ++ ++++ L+++ R SV I ++ S I+ G+ E + L EV KA S T+A S++ ++ + F IG+ N+ K+ K E + + GP +++ A+ ++ ++ D Y I +I + R + I +++I ++Y+V ++IP E N I + +K + N D++ E + G K I EI + I+ K I ++ P+ ++E K T ++++ V + V + ++F+ +IG G IKK+R I +P +NS +++TG + AA+ I+ +EL + + L +++P++ H LIG KG I I + V I PS+ S + +TI+G +VE+AK ++L + ++ L H LIG G N+ +++ + I+FP + ++ ++ + GT++SV EL
Sbjct: 144 LQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGTKEGIEKARHEILLISAEQDKRAVERLEIEKAYHPFIAGPYNKVVTEIMQETATRINI--PPPSVNKTEIVCTGEKEQLAQAVARIKQIYEEKKKKTTSIAVEVRKSQHKYVIGPKGNNLQEILERTGVSVEIPPSDSSSETVILRGEPEKLGQALTEVYAKANS-------FTVA-SVSAPSWLHR----FIIGKKGQNLAKITQQIPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALVRDLVKYFTCICVYVIHVYVLFLLVMRSV--IGLMDHIAVNKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRME-------------NERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRGPKNEVE------KCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANITE--------LEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEEKQTKSFTSELKAKPEYHKFLIGKGGGNIRKVRDQTGARIIFPT--ADDKDQQLITIVGTEDSVKEAQKEL 794 HSP 5 Score: 107.842 bits (268), Expect = 8.066e-23 Identity = 139/621 (22.38%), Postives = 278/621 (44.77%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG---KSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDV----DQVAAILQK-KHAFLVESNFSKKVKIPFKY-HKAIIG-REGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDD 2479
I +SG D V +A I+ + Q A+ + IPKE H+++IG G ++++ +T+ + I R E S N I + G K+ + A + L+ + + + ++ H FI G K + ++ + + NI ++ +IV G E++ + +K+ + + + + + +E+ K++IG G N+Q+I + T S+ P D + + V + G P + + + + H +IG++G+ L +I + P+V I F D ISL GP +DV Q+ A+++ F + V + F +++IG + I + ++ + +IP SE+++ I I G + V+ A K + + + N + I + H +IG+ G RI ++E V I FP P S+ V ++G K++V K L +V E+ ++Y + + ++ + +KK+RE + +I +E + + I G++ N + + + E+ I L ++I + R R M + + S + + RGPVQ+++ AK +++ + ++
Sbjct: 124 IMVSGKHDSVMKARKEIVTRLQTQASAS-----VAIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPEEAS-NQIKITGTKEGIEKARHEILLISAEQDKRAVERLEIEKAYHPFIAGPYNKVVTEIMQETATRINIPPPSVNK-TEIVCTGEKEQLAQAVARIKQIYEEKKKKTTSIAVEVRKSQHKYVIGPKGNNLQEILERTGVSVEIPPSDSSSETVILRGEPEKLGQALTEVYAKANSFTVASVSAPSWLHRF---IIGKKGQNLAKITQQIPKVHIEF---TEGEDKISLEGPTEDVTVAQQQMEALVRDLVKYFTCICVYVIHVYVLFLLVMRSVIGLMDHIAVNKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKF-PEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANIT--ELEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEE 726 HSP 6 Score: 98.2117 bits (243), Expect = 6.537e-20 Identity = 214/1003 (21.34%), Postives = 412/1003 (41.08%), Query Frame = 2
Query: 896 TICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQL-RFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIG----TPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAA----SEENDEIVITGKQKDV-------EDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGN-RINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASN-----YEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSH----LRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQG---ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTIS-NSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQD----------SNINE-----------------ITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQP 3706
+I + G D++ A + T+ ++ ++ P H+F+IGK+G L DL + IQ+ R E+ +NQI I G+ +E IE + + + Q + + +EI+ I G + +I +ET IN P + K + G V +K I + + + + H +IG +G L+EI +R V + PP D+ S+ + L G P K + + K ++F V S V P H+ IIG++G + ++T+ QIP +E D+I + G +DV E ++ + + + + + +IG + +N +KE +V++R P SN + I+G V +A+ +L +L + EQ F T + +++++RE + I D + +++ G K V + LQK SE+ V + ++S+ F K+ + G N+ K+ P E ++ G N ++A+D I+ I + N ITE + +L N ++ + + + I F E S T++ V K + + + L P + I G NI ++ + + I A +KD Q + I G +D + A++E+ L +++ ++V + + + +G+ ++ + + V + P + +V + G+ E V AAKK+I++ +E+LE ++ + ++HR ++G KG + +I + V+I P ++ N +E I I G K+ E A + + A V IEV + +H +IG +G+ + ++ +++V I P + ++ + + + G ++DR L++R + E DR + + + F P
Sbjct: 123 SIMVSGKHDSVMKARKEIVTRLQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGT-KEGIEKARHEILLISAEQDKRAVERLEIEKAYHPFIAGPYNKVVTEIMQETATRINIPPPSVNKTEIVCTGEKEQLAQAVARIKQIYEEKKKKTTSIAVEVRKSQHKY---VIGPKGNNLQEILERT-GVSVEIPPSDSSSETVILRGEPEKLGQALTEVYAKANSFTVAS-----VSAPSWLHRFIIGKKGQNLAKITQ------QIPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALVRDLVKYFTCICVYVIHVYVLFLLVMRSVIGLMDHIAVNKIKEQY--KVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRT------IIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYLQKMVSEL---VENSYSISVPIFKQFHKN--IIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELAN-ITELEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEEKQTKSFTSELKAKPEYHKFLI-------GKGGGNIRKVRDQTGARIIFPTADDKD--------QQLITIVGTEDSVKEAQKELEFLIKSLDNIVEDSMSVDPKHHRHFVIRRGQVLRDIAEEYGGVIVSFPRSGVQSDRVTLKGAKECVEAAKKKILDIIEDLE--------AQVTIECLITQRFHRSIMGPKGIRVQQITREFGVQIKFPDREVTPQGEPHVQENGDEGTEVKATDPESPKKCDIILISGRKEKCEAASDALKALV----PVSIEV-EVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVP---SPEQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHP 1064
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Xenopus
Match: hdlbp (high density lipoprotein binding protein (vigilin) [Source:NCBI gene;Acc:448610]) HSP 1 Score: 338.576 bits (867), Expect = 1.800e-114 Identity = 220/689 (31.93%), Postives = 383/689 (55.59%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIG---RDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
K YH N + +++ + R++IPP SV+K EI +G+++ +AQA + + Q Y + K + +E+ K +HKY+IGP+G+ ++EIL++T V V+I ++ S T+ L G + LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V + ++ V DL SR+ E+ ID +H+IG G I KI+++ S+ P+D K + I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+++FPEV INFP P SD++ L GP+ +V++ LQK + LVE+++S V I ++HK IIG+ G I+++ + NTK +PA + ++ IVITGK+ + E A R+ I ++ +NI E+ + I LH LIG G I S+ E CGG V I FPS + S+TV I+G DV +A+ +L L EE ++ + E ++FL ++K+R+ RI ++ +D+ + I+G +++V + L+ + IV +++++ +F + R G RD + + + + +G + +++AK +I+ II+D E +T ++ +R ++I ++ +I++++ VQI+ P
Sbjct: 232 KAYHPFIAGPYNKVVTEIMQETATRINIPPPSVNKTEIVCTGEKEQLAQAVARIKQIYEEKKKKTTSIAVEVRKSQHKYVIGPKGNNLQEILERTGVSVEIPP---SDSSSETVILRGEPEKLGQALTEVYAKANSFTVASVSAPSWLHRFIIGKKGQNLAKITQQIPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALVRDLISRMDFAEINIDHRFHRHLIGKSGAKINKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANITELEVNIPSKLHNSLIGAKGRFIRSIMEECGG-VHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEEKQTKSFTSELKAKPEYHKFLIGKGGGNIRKVRDQTGARIIFPTADDKDQQLITIVGTEDSVKEAQKELEFLIKSLDNIVEDSMSVDPKHHRHF-----VIRRGQVLRDIAEEYGGVIVSFPRSGVQSDRVTLKGAKECVEAAKKKILDIIEDLEAQVTIECLITQRFHRSIMGPKGIRVQQITREFGVQIKFP 911 HSP 2 Score: 97.4413 bits (241), Expect = 1.800e-114 Identity = 53/138 (38.41%), Postives = 79/138 (57.25%), Query Frame = 3
Query: 102 FITDTFVIPYEERRYIDSES---NKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
IT F +P EER+Y D + K+ +I G ++E ++ DQ L + +SG+ D V +A+K I +QTQ S+ IPKE HR +IG G ++LE T+T++ P+ + S IKI G+K+GIE AR I
Sbjct: 76 IITQVFHVPLEERKYKDMAQFGEGEQAKICLDIMQKTGAQLELSLAKDQGLSIMVSGKHDSVMKARKEIVTRLQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGTKEGIEKARHEI 213 HSP 3 Score: 172.17 bits (435), Expect = 1.264e-42 Identity = 105/330 (31.82%), Postives = 179/330 (54.24%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEI--NDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSW 3883
I+G K+K A + + L + +EV VP + + +IG KG I+K+ +V+I++P+ + + +TG S ++ AK ++E V EL+ +++R +R+F LA+ V ++H K+IG KG IS IR+ + V I P + D N ++I I G++ N E A++ +L V LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N+V V G E VD D L++ + ++ N+ MQ S+ ES QP + GF +R+APW N+ S+S+ + G P+ +TS W
Sbjct: 953 ISGRKEKCEAASDALKALVPVSIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSPEQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHPKIIGRKGAVISLIRSDHDVNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSEDITVDHRVHARIIGARGKAIRKIMDEFKVDIRFPQTG--APDPNLVTVTGKPEDVDEAIDHLLNLEEEYMSDVVDNEVMQSYMKPHSHEESKSQPSK-------------GFVVRDAPWTTGSNEKAPDMSSSEDFPIFG----TPVAHKTSPW 1263 HSP 4 Score: 127.102 bits (318), Expect = 1.138e-28 Identity = 141/618 (22.82%), Postives = 280/618 (45.31%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG---KSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSK----KVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDD 2479
I +SG D V +A I+ + Q A+ + IPKE H+++IG G ++++ +T+ + I R E S N I + G K+ + A + L+ + + + ++ H FI G K + ++ + + NI ++ +IV G E++ + +K+ + + + + + +E+ K++IG G N+Q+I + T S+ P D + + V + G P + + + + H +IG++G+ L +I + P+V I F D ISL GP +DV + Q++ LV S+ ++ I ++H+ +IG+ G I ++ + +IP SE+++ I I G + V+ A K + + + N + I + H +IG+ G RI ++E V I FP P S+ V ++G K++V K L +V E+ ++Y + + ++ + +KK+RE + +I +E + + I G++ N + + + E+ I L ++I + R R M + + S + + RGPVQ+++ AK +++ + ++
Sbjct: 128 IMVSGKHDSVMKARKEIVTRLQTQASAS-----VAIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPEEAS-NQIKITGTKEGIEKARHEILLISAEQDKRAVERLEIEKAYHPFIAGPYNKVVTEIMQETATRINIPPPSVNK-TEIVCTGEKEQLAQAVARIKQIYEEKKKKTTSIAVEVRKSQHKYVIGPKGNNLQEILERTGVSVEIPPSDSSSETVILRGEPEKLGQALTEVYAKANSFTVASVSAPSWLHRF---IIGKKGQNLAKITQQIPKVHIEF---TEGEDKISLEGPTEDV----TVAQQQMEALVRDLISRMDFAEINIDHRFHRHLIGKSGAKINKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKF-PEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANIT--ELEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEE 723 HSP 5 Score: 125.946 bits (315), Expect = 2.045e-28 Identity = 152/687 (22.13%), Postives = 300/687 (43.67%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXE-QGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLI-VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSST-ISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFV---LDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES-GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
+++ S V IP ++H+ +IG+ G +++L T TK QIP E +++I ITG ++ +E A + I ++ +EI K H + G + + + R+ I P P + ++ G K+ + +A ++ + EE ++ ++++ L+++ R SV I ++ S I+ G+ E + L EV KA S S++ ++ + F IG+ N+ K+ K E + + GP +++ A+ ++ ++ D + + I +D R +++ S ++++I ++Y+V ++IP E N I + +K + N D++ E + G K I EI + I+ K I ++ P+ ++E K T ++++ V + V + ++F+ +IG G IKK+R I +P +NS +++TG + AA+ I+ +EL + + L +++P++ H LIG KG I I + V I PS+ S + +TI+G +VE+AK ++L + ++ L H LIG G N+ +++ + I+FP + ++ ++ + GT++SV EL
Sbjct: 148 LQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGTKEGIEKARHEILLISAEQDKRAVERLEIEKAYHPFIAGPYNKVVTEIMQETATRINI--PPPSVNKTEIVCTGEKEQLAQAVARIKQIYEEKKKKTTSIAVEVRKSQHKYVIGPKGNNLQEILERTGVSVEIPPSDSSSETVILRGEPEKLGQALTEVYAKANS--------FTVASVSAPSWLHR----FIIGKKGQNLAKITQQIPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALVRDLISRMDFAEINIDHRFHRHLIGKSGAKINKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRME-------------NERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRGPKNEVE------KCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANITE--------LEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEEKQTKSFTSELKAKPEYHKFLIGKGGGNIRKVRDQTGARIIFPT--ADDKDQQLITIVGTEDSVKEAQKEL 791 HSP 6 Score: 113.62 bits (283), Expect = 1.472e-24 Identity = 222/1000 (22.20%), Postives = 417/1000 (41.70%), Query Frame = 2
Query: 896 TICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQL-RFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIG----TPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAA----SEENDEIVITGKQKDVEDAIKRV-CNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASN-----YEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSH----LRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQG---ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTIS-NSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQD--------------SNINE-----------------ITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQP 3706
+I + G D++ A + T+ ++ ++ P H+F+IGK+G L DL + IQ+ R E+ +NQI I G+ +E IE + + + Q + + +EI+ I G + +I +ET IN P + K + G V +K I + + + + H +IG +G L+EI +R V + PP D+ S+ + L G P K + + K ++F V S V P H+ IIG++G + ++T+ QIP +E D+I + G +DV A +++ + + S + I I H LIGK G +IN +KE +V++R P SN + I+G V +A+ +L +L + EQ F T + +++++RE + I D + +++ G K V + LQK SE+ V + ++S+ F K+ + G N+ K+ P E ++ G N ++A+D I+ I + N ITE + +L N ++ + + + I F E S T++ V K + + + L P + I G NI ++ + + I A +KD Q + I G +D + A++E+ L +++ ++V + + + +G+ ++ + + V + P + +V + G+ E V AAKK+I++ +E+LE ++ + ++HR ++G KG + +I + V+I P ++ N +E I I G K+ E A + + A V IEV + +H +IG +G+ + ++ +++V I P + ++ + + + G ++DR L++R + E DR + + + F P
Sbjct: 127 SIMVSGKHDSVMKARKEIVTRLQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGT-KEGIEKARHEILLISAEQDKRAVERLEIEKAYHPFIAGPYNKVVTEIMQETATRINIPPPSVNKTEIVCTGEKEQLAQAVARIKQIYEEKKKKTTSIAVEVRKSQHKY---VIGPKGNNLQEILERT-GVSVEIPPSDSSSETVILRGEPEKLGQALTEVYAKANSFTVAS-----VSAPSWLHRFIIGKKGQNLAKITQ------QIPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALVRDLISRMDFAEINIDHRFHRHLIGKSGAKINKIKEQY--KVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRT------IIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYLQKMVSEL---VENSYSISVPIFKQFHKN--IIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELAN-ITELEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEEKQTKSFTSELKAKPEYHKFLI-------GKGGGNIRKVRDQTGARIIFPTADDKD--------QQLITIVGTEDSVKEAQKELEFLIKSLDNIVEDSMSVDPKHHRHFVIRRGQVLRDIAEEYGGVIVSFPRSGVQSDRVTLKGAKECVEAAKKKILDIIEDLE--------AQVTIECLITQRFHRSIMGPKGIRVQQITREFGVQIKFPDREVTPQGAPNAEPHVQENGDEGTEVKATDPESPKKCDIILISGRKEKCEAASDALKALV----PVSIEV-EVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVP---SPEQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHP 1065 HSP 7 Score: 93.2041 bits (230), Expect = 2.146e-18 Identity = 133/563 (23.62%), Postives = 249/563 (44.23%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA----LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRF--EDEANQ--IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSI-NFPRD-LTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFP--------PPDADSDL----------------------ISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDA----IKRVCNIYXXXXXXXXXXXXXXXX----LHTILIGKGGNRINSLKESCGGRVTIRFPSP-DADSNTVIIKG-------VKDDVLKAQVKLHDLVEEITASNYEQHFQM 2080
K++H+ K + + E + ++D+P + + I I+G R A +L ++ A EL++ IP + H +IG +G I+ I+++ V I+ GS +T+ + GP ++ A L L K + A HKF+IGK G N+ + ++ ++ F D+ +Q I I G+ + V E + L+ + L + + + M +DP +H + G ++ I +E I +FPR + VT+ G VEA K +L IE +E + + T ++G +G +++I F V+I FP P+A+ + I LI RK+ + A+ A S +V++PF H+ IIG++G IR++ Q+P+ ++ D I ITG+ +++ A ++RV + ++ + +++ + H +IG+ G I+ ++ V I+FP D + + +II G +D +L L +V E ++ H ++
Sbjct: 591 KQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKEL-ANITELEVNIPSKLHNSLIGAKGRFIRSIMEECG-GVHIHFPSEGSGS-DTVTIRGPVQDVERAKKQLLQLAEEKQTKSFTSELKAKPEYHKFLIGKGGGNIRKVR-DQTGARIIFPTADDKDQQLITIVGTEDSVKEAQKELEFLIKSLDN-IVEDSMSVDPKHHRHFVIRRGQVLRDIAEEYGGVIVSFPRSGVQSDRVTLKGAKECVEAAKKKILDIIEDLEAQVTIECLITQRFHRSIMGPKGIRVQQITREF-GVQIKFPDREVTPQGAPNAEPHVQENGDEGTEVKATDPESPKKCDIILISGRKEKCEAAS-----DALKALVPVSIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSPEQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHPKIIGRKGAVISLIRSDHD--VNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSEDITVDHRVHARI 1140 HSP 8 Score: 77.411 bits (189), Expect = 1.798e-13 Identity = 82/357 (22.97%), Postives = 156/357 (43.70%), Query Frame = 2
Query: 635 LDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLN-EGSPNT-------------ICLLGPKDNLGPALTLVFTKSESVNI------------IAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP--RDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDA-DSDLISLIGPRKDVDQV 1573
+ P V + +++ G ++ V A +L ++A ++ I + H+ I+GP+G +++I + V + ++ + +G+PN + P+ + L+ + E I ++ P LH++IIG+ GS + V++ V P E + + I I G + L ++V +LQ+ R +L + +DP IIG G I IR + + +I FP D + + I G E+ + +L + +E +DI H + ++IG +GK +++I D F +V I FP A D +L+++ G +DVD+
Sbjct: 836 VSFPRSGVQSDRVTLKGAKECVEAAKKKILDIIEDLEAQVT-IECLITQRFHRSIMGPKGIRVQQITREFGVQIKFPDREVTPQGAPNAEPHVQENGDEGTEVKATDPESPKKCDIILISGRKEKCEAASDALKALVPVSIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSP------EQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHPKIIGRKGAVISLIRSDHDVNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSEDITVDHRVHARIIGARGKAIRKIMDEF-KVDIRFPQTGAPDPNLVTVTGKPEDVDEA 1184 HSP 9 Score: 65.0846 bits (157), Expect = 1.047e-9 Identity = 66/258 (25.58%), Postives = 120/258 (46.51%), Query Frame = 2
Query: 647 PFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNI-----------IAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDE----ANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLT--KKVVTIIGTPHGV-EAVKAIL 1366
P S K +I + R +AAS L+ + +++E+P + H+YIIG +GSGI++++D+ V + + E +TI + G NL A + + + +A+ H IIG+ G+ ++ L + ++ ++F D+ +QI+I G + + V DL+ +S+ ++ +D + IIG+ G I+KI E I FP+ +VT+ G P V EA+ +L
Sbjct: 942 PESPKKCDIILISGRKEKCEAASDALKALVPVS-----IEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSP---EQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHPKIIGRKGA-VISLIRSDHDVNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSE-DITVDHRVHARIIGARGKAIRKIMDEFKVDIRFPQTGAPDPNLVTVTGKPEDVDEAIDHLL 1189
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Xenopus
Match: hdlbp (high density lipoprotein binding protein (vigilin) [Source:NCBI gene;Acc:448610]) HSP 1 Score: 280.796 bits (717), Expect = 3.915e-97 Identity = 178/497 (35.81%), Postives = 286/497 (57.55%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLM-IKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEE 2044
K YH N + +++ + R++IPP SV+K EI +G+++ +AQA + + Q Y + K + +E+ K +HKY+IGP+G+ ++EIL++T V V+I ++ S T+ L G + LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V + ++ V DL + D L+ +H KI+++ S+ P+D K + I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+++FPEV INFP P SD++ L GP+ +V++ LQK + LVE+++S V I ++HK IIG+ G I+++ + NTK +PA + ++ IVITGK+ + E A R+ I ++ +NI E+ + I LH LIG G I S+ E CGG V I FPS + S+TV I+G DV +A+ +L L EE
Sbjct: 228 KAYHPFIAGPYNKVVTEIMQETATRINIPPPSVNKTEIVCTGEKEQLAQAVARIKQIYEEKKKKTTSIAVEVRKSQHKYVIGPKGNNLQEILERTGVSVEIPP---SDSSSETVILRGEPEKLGQALTEVYAKANSFTVASVSAPSWLHRFIIGKKGQNLAKITQQIPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALVRDLVKYFALHFSFFDHLLNPRH-------QFNKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANITELEVNIPSKLHNSLIGAKGRFIRSIMEECGG-VHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEE 713 HSP 2 Score: 97.4413 bits (241), Expect = 3.915e-97 Identity = 58/156 (37.18%), Postives = 86/156 (55.13%), Query Frame = 3
Query: 102 FITDTFVIPYEERRYIDSES---NKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIE 560
IT F +P EER+Y D + K+ +I G ++E ++ DQ L + +SG+ D V +A+K I +QTQ S+ IPKE HR +IG G ++LE T+T++ P+ + S IKI G+K+GIE AR I E K + +EIE
Sbjct: 72 IITQVFHVPLEERKYKDMAQFGEGEQAKICLDIMQKTGAQLELSLAKDQGLSIMVSGKHDSVMKARKEIVTRLQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGTKEGIEKARHEILLISAEQDKRAVERLEIE 227 HSP 3 Score: 172.94 bits (437), Expect = 6.393e-43 Identity = 105/330 (31.82%), Postives = 179/330 (54.24%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEI--NDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSW 3883
I+G K+K A + + L + +EV VP + + +IG KG I+K+ +V+I++P+ + + +TG S ++ AK ++E V EL+ +++R +R+F LA+ V ++H K+IG KG IS IR+ + V I P + D N ++I I G++ N E A++ +L V LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N+V V G E VD D L++ + ++ N+ MQ S+ ES QP + GF +R+APW N+ S+S+ + G P+ +TS W
Sbjct: 791 ISGRKEKCEAASDALKALVPVSIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSPEQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHPKIIGRKGAVISLIRSDHDVNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSEDITVDHRVHARIIGARGKAIRKIMDEFKVDIRFPQTG--APDPNLVTVTGKPEDVDEAIDHLLNLEEEYMSDVVDNEVMQSYMKPHSHEESKSQPSK-------------GFVVRDAPWTTGSNEKAPDMSSSEDFPIFG----TPVAHKTSPW 1101 HSP 4 Score: 110.153 bits (274), Expect = 1.496e-23 Identity = 136/614 (22.15%), Postives = 272/614 (44.30%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG---KSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDD 2479
I +SG D V +A I+ + Q A+ + IPKE H+++IG G ++++ +T+ + I R E S N I + G K+ + A + L+ + + + ++ H FI G K + ++ + + NI ++ +IV G E++ + +K+ + + + + + +E+ K++IG G N+Q+I + T S+ P D + + V + G P + + + + H +IG++G+ L +I + P+V I F D ISL GP +DV + Q++ LV + K + F + ++ ++ + +IP SE+++ I I G + V+ A K + + + N + I + H +IG+ G RI ++E V I FP P S+ V ++G K++V K L +V E+ ++Y + + ++ + +KK+RE + +I +E + + I G++ N + + + E+ I L ++I + R R M + + S + + RGPVQ+++ AK +++ + ++
Sbjct: 124 IMVSGKHDSVMKARKEIVTRLQTQASAS-----VAIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPEEAS-NQIKITGTKEGIEKARHEILLISAEQDKRAVERLEIEKAYHPFIAGPYNKVVTEIMQETATRINIPPPSVNK-TEIVCTGEKEQLAQAVARIKQIYEEKKKKTTSIAVEVRKSQHKYVIGPKGNNLQEILERTGVSVEIPPSDSSSETVILRGEPEKLGQALTEVYAKANSFTVASVSAPSWLHRF---IIGKKGQNLAKITQQIPKVHIEF---TEGEDKISLEGPTEDV----TVAQQQMEALVR-DLVKYFALHFSFFDHLLNPRH-QFNKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKF-PEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANIT--ELEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEE 713 HSP 5 Score: 103.605 bits (257), Expect = 1.387e-21 Identity = 136/614 (22.15%), Postives = 264/614 (43.00%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXE-QGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSST-ISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFV---LDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFV 3400
+++ S V IP ++H+ +IG+ G +++L T TK QIP E +++I ITG ++ +E A + I ++ +EI K H + G + + + R+ I P P + ++ G K+ + +A ++ + EE ++ ++++ L+++ R SV I ++ S I+ G+ E + L EV KA S T+A S++ ++ + F IG+ N+ K+ K E + + GP +++ A+ ++ ++ D + + L ++Q ++I ++Y+V ++IP E N I + +K + N D++ E + G K I EI + I+ K I ++ P+ ++E K T ++++ V + V + ++F+ +IG G IKK+R I +P +NS +++TG + AA+ I+ +EL + + L +++P++ H LIG KG I I + V I PS+ S + +TI+G +VE+AK ++L
Sbjct: 144 LQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGTKEGIEKARHEILLISAEQDKRAVERLEIEKAYHPFIAGPYNKVVTEIMQETATRINI--PPPSVNKTEIVCTGEKEQLAQAVARIKQIYEEKKKKTTSIAVEVRKSQHKYVIGPKGNNLQEILERTGVSVEIPPSDSSSETVILRGEPEKLGQALTEVYAKANS-------FTVA-SVSAPSWLHR----FIIGKKGQNLAKITQQIPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALVRDLVKYFALHFSFFDHLLN-----PRHQFNKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRME-------------NERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRGPKNEVE------KCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANITE--------LEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLA 711 HSP 6 Score: 92.0485 bits (227), Expect = 4.927e-18 Identity = 72/264 (27.27%), Postives = 143/264 (54.17%), Query Frame = 2
Query: 2873 NIKLPQ-----YKLEITGHKDKITLAKEEIIKLFVL-----DLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRA 3625
++++PQ + I G + AK+E+++L ++ + ++F+ +IG KGE+I+++R +V I P+ K V + G K E+ + + L++M E ++S+++ + Q+H+ +IG G I KIR + + +I +P+++SN I I G + N E A++ +LA +L + I E+ +NI S++H+ LIG++G + + ++ V I FP +EG ++ V ++G + V+R +L+ A
Sbjct: 459 SVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRG-------PKNEVEKCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKEL-ANITELEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFP---SEGSGSDTVTIRGPVQDVERAKKQLLQLA 711 HSP 7 Score: 75.0998 bits (183), Expect = 8.270e-13 Identity = 138/554 (24.91%), Postives = 236/554 (42.60%), Query Frame = 2
Query: 602 LHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMK-ATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALT-LVFTKSESV-NIIAIDAPGW--LHKFIIGKSGSNL----------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRL---SQLEMEIDPLMIKHIIGSGGVNIQKIRKETN-CSINFPRDLT-KKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMP----QLIGQQG---------------------KGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDA----IKRVCNIYXXXXXXXXXXXXXXXX----LHTILIGKGGNRINSLKESCGGRVTIRFPSP-DADSNTVIIKG-------VKDDVLKAQVKLHDLVEEITASNYEQHFQM 2080
+ + E+ + + IP S N I I GD GV QA LL+ ++M+ ++L IE ++ H+ IIG +G I+EI ++ V IN + S + + L GPK+ + L SE V N +I P + HK IIGK G+N+ +DL N ++ IVI G E + ++ +Q L ++LE+ I + +IG+ G I+ I +E I+FP + + VTI G VE K LL+ E + + P L+ Q ++E D EV+ P D+I LI RK+ + A+ A S +V++PF H+ IIG++G IR++ Q+P+ ++ D I ITG+ +++ A ++RV + ++ + +++ + H +IG+ G I+ ++ V I+FP D + + +II G +D +L L +V E ++ H ++
Sbjct: 449 FNKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIE--QKFHRTIIGQKGERIREIREKFP-EVIINFPDPAQKS-DIVQLRGPKNEVEKCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEM--------IVITGKRPNC----EAARDRILAIQKELANITELEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEEKVRSVQYINRLNFYFFPFPRITLLRIQCLHHIYSFLHFFFFFFLAPNAEPHVQENGDEGTEVKATDPESPKKCDII-LISGRKEKCEAAS-----DALKALVPVSIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSPEQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHPKIIGRKGAVISLIRSDHD--VNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSEDITVDHRVHARI 978 HSP 8 Score: 71.633 bits (174), Expect = 9.091e-12 Identity = 134/562 (23.84%), Postives = 238/562 (42.35%), Query Frame = 2
Query: 896 TICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQL-RFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIG----TPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAA----SEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGN---RINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASN-----YEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSH----LRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQG---ILEFRGPVQNIKSAKDEIIHIIDDYENTITE 2500
+I + G D++ A + T+ ++ ++ P H+F+IGK+G L DL + IQ+ R E+ +NQI I G+ +E IE + + + Q + + +EI+ I G + +I +ET IN P + K + G V +K I + + + + H +IG +G L+EI +R V + PP D+ S+ + L G P K + + K ++F V S V P H+ IIG++G + ++T+ QIP +E D+I + G +DV A +++ + + LH N + N +KE +V++R P SN + I+G V +A+ +L +L + EQ F T + +++++RE + I D + +++ G K V + LQK SE+ V + ++S+ F K+ + G N+ K+ P E ++ G N ++A+D I+ I + N ITE
Sbjct: 123 SIMVSGKHDSVMKARKEIVTRLQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGT-KEGIEKARHEILLISAEQDKRAVERLEIEKAYHPFIAGPYNKVVTEIMQETATRINIPPPSVNKTEIVCTGEKEQLAQAVARIKQIYEEKKKKTTSIAVEVRKSQHKY---VIGPKGNNLQEILERT-GVSVEIPPSDSSSETVILRGEPEKLGQALTEVYAKANSFTVAS-----VSAPSWLHRFIIGKKGQNLAKITQ------QIPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALV--------RDLVKYFALHFSFFDHLLNPRHQFNKIKEQY--KVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRT------IIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYLQKMVSEL---VENSYSISVPIFKQFHKN--IIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELAN-ITE 646 HSP 9 Score: 71.2478 bits (173), Expect = 1.244e-11 Identity = 58/218 (26.61%), Postives = 111/218 (50.92%), Query Frame = 2
Query: 2975 VNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFT----KVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
V+ P + +IG KG+ + K+ T Q IP + +FT K+ + G +E V A++++ V +L ++ F ALH H + N +KI+ + V + +P N I I+G V++AK E+L +++E+ + L I+ + H +IG +G + ++++K+ +V I FP ++++IV+++G K V++C L
Sbjct: 366 VSAPSWLHRFIIGKKGQNLAKI--TQQ----IPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALVRDL--------VKYF--ALHFSFFDH---LLNPRHQFNKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFP---DPAQKSDIVQLRGPKNEVEKCTKYL 561 HSP 10 Score: 68.9366 bits (167), Expect = 5.664e-11 Identity = 61/217 (28.11%), Postives = 104/217 (47.93%), Query Frame = 2
Query: 977 IAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP--RDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDA-DSDLISLIGPRKDVDQV 1573
I ++ P LH++IIG+ GS + V++ V P E + + I I G + L ++V +LQ+ R +L + +DP IIG G I IR + + +I FP D + + I G E+ + +L + +E +DI H + ++IG +GK +++I D F +V I FP A D +L+++ G +DVD+
Sbjct: 813 IEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSP------EQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHPKIIGRKGAVISLIRSDHDVNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSEDITVDHRVHARIIGARGKAIRKIMDEF-KVDIRFPQTGAPDPNLVTVTGKPEDVDEA 1022 HSP 11 Score: 65.0846 bits (157), Expect = 8.726e-10 Identity = 66/258 (25.58%), Postives = 120/258 (46.51%), Query Frame = 2
Query: 647 PFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNI-----------IAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDE----ANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLT--KKVVTIIGTPHGV-EAVKAIL 1366
P S K +I + R +AAS L+ + +++E+P + H+YIIG +GSGI++++D+ V + + E +TI + G NL A + + + +A+ H IIG+ G+ ++ L + ++ ++F D+ +QI+I G + + V DL+ +S+ ++ +D + IIG+ G I+KI E I FP+ +VT+ G P V EA+ +L
Sbjct: 780 PESPKKCDIILISGRKEKCEAASDALKALVPVS-----IEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSP---EQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHPKIIGRKGA-VISLIRSDHDVNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSE-DITVDHRVHARIIGARGKAIRKIMDEFKVDIRFPQTGAPDPNLVTVTGKPEDVDEAIDHLL 1027 HSP 12 Score: 63.5438 bits (153), Expect = 3.055e-9 Identity = 37/144 (25.69%), Postives = 73/144 (50.69%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFP 3529
++V+G +SV+ A+KEIV ++ S ++ +P ++HR +IG GE + + K + +I +P + N+I I G K+ +EKA++E+L + + +E L I+ H + G + ++ ++ I P
Sbjct: 124 IMVSGKHDSVMKARKEIVTRLQ-----------TQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGTKEGIEKARHEILLISAEQDKRAVERLEIEKAYHPFIAGPYNKVVTEIMQETATRINIP 256
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Xenopus
Match: hdlbp (high density lipoprotein binding protein (vigilin) [Source:NCBI gene;Acc:448610]) HSP 1 Score: 323.168 bits (827), Expect = 4.005e-91 Identity = 285/1009 (28.25%), Postives = 508/1009 (50.35%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAA-SILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFT----KSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIG---RDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP---FYP--EP-VNSKTISNSHVSAKPGEKPYMNGV-------DXXXXXXXXXXXXXP------NPNHLPNDIMSEHNSNGSKYHNISEINSEVSS------TIS-AGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFT-KVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS-NINEITIQGHKDNVEKAKNEVLAFVNKLESGII--EVLNIDSRIHS 3457
K +H+ K+ L + + + ++ IP N+I I+G ++G+ +A ILL + Q K + L+IE K H +I GP + EI+ +T+ ++I +N+ I G K+ L A+ + K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V + ++ V DL SR+ E+ ID +H+IG G I KI+++ S+ P+D K + I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+++FPEV INFP P SD++ L GP+ +V++ LQK + LVE+++S V I ++HK IIG+ G I+++ + NTK +PA + ++ IVITGK+ + E A R+ I ++ +NI E+ + I LH LIG G I S+ E CGG V I FPS + S+TV I+G DV +A+ +L L EE ++ + E ++FL ++K+R+ RI ++ +D+ + I+G +++V + L+ + IV +++++ +F + R G RD + + + + +G + +++AK +I+ II+D E +T ++ +R ++I ++ +I++++ VQI+ P P EP V + V A E P + + + ++ K+L P P L I+ + S K + E+N +V S TI+ G + N D L + + E+ ++ + ++ F L + V+ +F+P +IG KG I +R+ H V+I+ P+KN + ++I+TG + +A+ I+ V +LE+M S + V + H ++IG +G+ I KI + V I P + + N +T+ G ++V++A + +L + S ++ EV+ + HS
Sbjct: 156 KEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGTKEGIEKARHEILLISAEQDKRAVERLEIE--KAYHPFIAGPYNKVVTEIMQETATRINIPPPSVNK---TEIVCTGEKEQLAQAVARIKQIYEEKANSFTVASVSAPSWLHRFIIGKKGQNLAKITQQIPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALVRDLISRMDFAEINIDHRFHRHLIGKSGAKINKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANITELEVNIPSKLHNSLIGAKGRFIRSIMEECGG-VHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEEKQTKSFTSELKAKPEYHKFLIGKGGGNIRKVRDQTGARIIFPTADDKDQQLITIVGTEDSVKEAQKELEFLIKSLDNIVEDSMSVDPKHHRHF-----VIRRGQVLRDIAEEYGGVIVSFPRSGVQSDRVTLKGAKECVEAAKKKILDIIEDLEAQVTIECLITQRFHRSIMGPKGIRVQQITREFGVQIKFPDREVTPQGEPHVQENGDEGTEVKATDPESPKKCDIILISGRKEKCEAASDALKALVPVSIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSPEQQCDTITITGQSANLDRAKAGLLERVR-ELQAEQE------DRALRSFKLAVSVD--PKFHPKIIGRKGAVISLIRSDHDVNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKM--------VSEDITVDHRVHARIIGARGKAIRKIMDEFKVDIRFPQTGAPDPNLVTVTGKPEDVDEAIDHLLNLEEEYMSDVVDNEVMQSYMKPHS 1136 HSP 2 Score: 172.555 bits (436), Expect = 1.058e-42 Identity = 105/330 (31.82%), Postives = 179/330 (54.24%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEI--NDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSW 3883
I+G K+K A + + L + +EV VP + + +IG KG I+K+ +V+I++P+ + + +TG S ++ AK ++E V EL+ +++R +R+F LA+ V ++H K+IG KG IS IR+ + V I P + D N ++I I G++ N E A++ +L V LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N+V V G E VD D L++ + ++ N+ MQ S+ ES QP + GF +R+APW N+ S+S+ + G P+ +TS W
Sbjct: 876 ISGRKEKCEAASDALKALVPVSIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSPEQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHPKIIGRKGAVISLIRSDHDVNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSEDITVDHRVHARIIGARGKAIRKIMDEFKVDIRFPQTG--APDPNLVTVTGKPEDVDEAIDHLLNLEEEYMSDVVDNEVMQSYMKPHSHEESKSQPSK-------------GFVVRDAPWTTGSNEKAPDMSSSEDFPIFG----TPVAHKTSPW 1186 HSP 3 Score: 137.502 bits (345), Expect = 6.191e-32 Identity = 167/694 (24.06%), Postives = 307/694 (44.24%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRET---YSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFR--IGRD----NMCKLNVXXXXXXXXXXEQG-ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIPFYPEPVNSKTISNSHVSAKPGEK--PYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN--GSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINE-ITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIV-FPIRGTEGEEANIVKVKGTKESVDRCADELVD 3619
+++ S V IP ++H+ +IG+ G +++L T TK QIP E +++I ITG ++ +E A + I ++ +EI K H + G + + + R+ I P P + ++ G K+ + +A ++ + EE S + F+ L K+ + + +E EDK L G E+V A+ +M +V + + S D + R IG+ N K +E+ + G Q ++ AK E++ + EN T++LI++++ + I ++ EI +K+ +V I +P+P I EK Y+ + L N + I + + N G NI +I E ++ I A N SN ++ + ITG + A++ I+ + + +LEVN+P + + +LIGAKG I+ + V I P++ S V + G + V AKK++++ EE ++ ++F+ L +YH+ LIG G I K+R + RI P+ D + ITI G +D+V++A+ E+ + L++ + + +++D + H + RG L + ++Y IV FP G + + V +KG KE V+ +++D
Sbjct: 144 LQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGTKEGIEKARHEILLISAEQDKRAVERLEIEKAYHPFIAGPYNKVVTEIMQETATRINI--PPPSVNKTEIVCTGEKEQLAQAVARIKQIYEEKANSFTVASVSAPSWLHRFIIGKKGQNLAKITQQIPKVHIEFTEGEDKISLE--GPTEDVTV-------AQQQMEALVRDLI--SRMDFAEINIDHRFHRHLIGKSGAKINKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIIN---FPDPAQKSDIVQLRGPKNEVEKCTKYLQKM---------VSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLP-AEN----SNSEM----IVITGKRPNCEAARDRILAIQKELANITELEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEE-------KQTKSFTSELKAKPEYHKFLIGKGGGNIRKVRDQTGARIIFPTADDKDQQLITIVGTEDSVKEAQKELEFLIKSLDNIVEDSMSVDPKHHRHFVIRRGQVLRDIAEEYGGVIVSFPRSGVQSDR---VTLKGAKECVEAAKKKILD 793 HSP 4 Score: 114.005 bits (284), Expect = 1.031e-24 Identity = 129/542 (23.80%), Postives = 244/542 (45.02%), Query Frame = 2
Query: 896 TICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQL-RFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIG----TPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSK----KVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDD 2479
+I + G D++ A + T+ ++ ++ P H+F+IGK+G L DL + IQ+ R E+ +NQI I G+ +E IE + + + Q + + +EI+ I G + +I +ET IN P + K + G V +K I + + + H +IG++G+ L +I + P+V I F D ISL GP +DV + Q++ LV S+ ++ I ++H+ +IG+ G I ++ + +IP SE+++ I I G + V+ A K + + + N + I + H +IG+ G RI ++E V I FP P S+ V ++G K++V K L +V E+ ++Y + + ++ + +KK+RE + +I +E + + I G++ N + + + E+ I L ++I + R R M + + S + + RGPVQ+++ AK +++ + ++
Sbjct: 123 SIMVSGKHDSVMKARKEIVTRLQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGT-KEGIEKARHEILLISAEQDKRAVERLEIEKAYHPFIAGPYNKVVTEIMQETATRINIPPPSVNKTEIVCTGEKEQLAQAVARIKQIYEEKANSFTVASVSAPSWLHRF---IIGKKGQNLAKITQQIPKVHIEF---TEGEDKISLEGPTEDV----TVAQQQMEALVRDLISRMDFAEINIDHRFHRHLIGKSGAKINKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKF-PEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANIT--ELEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEE 650 HSP 5 Score: 102.449 bits (254), Expect = 3.694e-21 Identity = 139/666 (20.87%), Postives = 288/666 (43.24%), Query Frame = 2
Query: 1667 GREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRIS---EAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXE-QGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLI-VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSST-ISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFV---LDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES-GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
G + ++ + T + ++ A ++ I+++GK V A K I + + I K H +IGK G ++ L+ ++ I P P+ SN + I G K+ + KA+ ++ + E E+ ++ + F+ + + ++ + + RI+ + +K+ + G+KE + + +++ E T S++ ++ + F IG+ N+ K+ K E + + GP +++ A+ ++ ++ D + + I +D R +++ S ++++I ++Y+V ++IP E N I + +K + N D++ E + G K I EI + I+ K I ++ P+ ++E K T ++++ V + V + ++F+ +IG G IKK+R I +P +NS +++TG + AA+ I+ +EL + + L +++P++ H LIG KG I I + V I PS+ S + +TI+G +VE+AK ++L + ++ L H LIG G N+ +++ + I+FP + ++ ++ + GT++SV EL
Sbjct: 95 GEQAKICLDIMQKTGAQLELSLAKDQGLSIMVSGKHDSVMKARK---EIVTRLQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPEEASNQIKITGTKEGIEKARHEILLISAEQDKRAVER-LEIEKAYHPFIAGPYNKVVTEIMQETATRINIPPPSVNKTEIVCTGEKEQLAQAVARIKQIYEEKANSFT---VASVSAPSWLHR----FIIGKKGQNLAKITQQIPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALVRDLISRMDFAEINIDHRFHRHLIGKSGAKINKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRME-------------NERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRGPKNEVE------KCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANITE--------LEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPSEGSGSDTVTIRGPVQDVERAKKQLLQLAEEKQTKSFTSELKAKPEYHKFLIGKGGGNIRKVRDQTGARIIFPT--ADDKDQQLITIVGTEDSVKEAQKEL 718 HSP 6 Score: 97.4413 bits (241), Expect = 1.126e-19 Identity = 53/138 (38.41%), Postives = 79/138 (57.25%), Query Frame = 3
Query: 102 FITDTFVIPYEERRYIDSES---NKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
IT F +P EER+Y D + K+ +I G ++E ++ DQ L + +SG+ D V +A+K I +QTQ S+ IPKE HR +IG G ++LE T+T++ P+ + S IKI G+K+GIE AR I
Sbjct: 72 IITQVFHVPLEERKYKDMAQFGEGEQAKICLDIMQKTGAQLELSLAKDQGLSIMVSGKHDSVMKARKEIVTRLQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGTKEGIEKARHEI 209 HSP 7 Score: 91.6633 bits (226), Expect = 7.884e-18 Identity = 72/264 (27.27%), Postives = 143/264 (54.17%), Query Frame = 2
Query: 2873 NIKLPQ-----YKLEITGHKDKITLAKEEIIKLFVL-----DLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRA 3625
++++PQ + I G + AK+E+++L ++ + ++F+ +IG KGE+I+++R +V I P+ K V + G K E+ + + L++M E ++S+++ + Q+H+ +IG G I KIR + + +I +P+++SN I I G + N E A++ +LA +L + I E+ +NI S++H+ LIG++G + + ++ V I FP +EG ++ V ++G + V+R +L+ A
Sbjct: 396 SVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRG-------PKNEVEKCTKYLQKMVSELVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKEL-ANITELEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFP---SEGSGSDTVTIRGPVQDVERAKKQLLQLA 648
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Xenopus
Match: hdlbp (high density lipoprotein binding protein (vigilin) [Source:NCBI gene;Acc:448610]) HSP 1 Score: 266.544 bits (680), Expect = 3.938e-73 Identity = 260/950 (27.37%), Postives = 460/950 (48.42%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPAL-TLVFTKSESV-----------NIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIG---RDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGV-------DXXXXXXXXXXXXXP------NPNHLPNDIMSEHNSNGSKYHNISEINSEVSS------TIS-AGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFT-KVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS-NINEITIQGHKDNVEKAKNEVL 3391
I +SG D V +A I+ + Q A+ + IPKE H+++IG G ++++ +T+ + I R E S N I + G K+ + A ++ +E V + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V + ++ V DL SR+ E+ ID +H+IG G I KI+++ S+ P+D K + I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+++FPEV INFP P SD++ L GP+ +V++ LQK +VE+++S V I ++HK IIG+ G I+++ + NTK +PA + ++ IVITGK+ + E A R+ I ++ +NI E+ + I LH LIG G I S+ E CGG V I FPS E + +NY + + E ++FL ++K+R+ RI ++ +D+ + I+G +++V + L+ + IV +++++ +F + R G RD + + + + +G + +++AK +I+ II+D ++ +R ++I ++ +I++++ VQI+ P I + V A E P + + + ++ K+L P P L I+ + S K + E+N +V S TI+ G + N D L + + E+ A++E L L V+V +F+P +IG KG I +R+ H V+I+ P+KN + ++I+TG + +A+ I+ V +LE+M E + V + H ++IG +G+ I KI + V I P + + N +T+ G ++V++A + +L
Sbjct: 63 IMVSGKHDSVMKARKEIVTRLQTQASAS-----VAIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPEEAS-NQIKITGTKEGIEKARHEILLISAEQVKNICTFFPFLFTVASVSAPSWLHRFIIGKKGQNLAKITQQIPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALVRDLISRMDFAEINIDHRFHRHLIGKSGAKINKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYLQK----MVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANITELEVNIPSKLHNSLIGAKGRFIRSIMEECGG-VHIHFPS-----------------------------EGSGTNYAE-LKAKPEYHKFLIGKGGGNIRKVRDQTGARIIFPTADDKDQQLITIVGTEDSVKEAQKELEFLIKSLDNIVEDSMSVDPKHHRHF-----VIRRGQVLRDIAEEYGGVIVSFPRSGVQSDRVTLKGAKECVEAAKKKILDIIEDL-------CLITQRFHRSIMGPKGIRVQQITREFGVQIKFPDREVTPQGGEILGTEVKATDPESPKKCDIILISGRKEKCEAASDALKALVPVSIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSPEQQCDTITITGQSANLDRAKAGLLERVR-ELQ--------AEQEDRALRSFKLAVSVDPKFHPKIIGRKGAVISLIRSDHDVNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSE--------DITVDHRVHARIIGARGKAIRKIMDEFKVDIRFPQTGAPDPNLVTVTGKPEDVDEAIDHLL 940 HSP 2 Score: 151.754 bits (382), Expect = 2.018e-36 Identity = 83/240 (34.58%), Postives = 143/240 (59.58%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVD 3619
I+G K+K A + + L + +EV VP + + +IG KG I+K+ +V+I++P+ + + +TG S ++ AK ++E V EL+ +++R +R+F LA+ V ++H K+IG KG IS IR+ + V I P + D N ++I I G++ N E A++ +L V LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N+V V G E VD D L++
Sbjct: 704 ISGRKEKCEAASDALKALVPVSIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSPEQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHPKIIGRKGAVISLIRSDHDVNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSEDITVDHRVHARIIGARGKAIRKIMDEFKVDIRFPQTG--APDPNLVTVTGKPEDVDEAIDHLLN 941 HSP 3 Score: 113.235 bits (282), Expect = 1.483e-24 Identity = 173/752 (23.01%), Postives = 319/752 (42.42%), Query Frame = 2
Query: 563 RYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMK-ATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNL----------VDLSVNKPNIQL------RFEDEANQIVIEGSPEEVIEVSEY-------LKKKVFDLQSRL----------------------SQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR--DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTK---------------------ATNTKFQIPAASEENDEIVITGKQKDVE---DAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
R+H+ KS ++ + E+ + + IP S N I I GD GV QA LL+ ++M+ ++L IE ++ H+ IIG +G I+EI ++ V IN + S + + L GPK+ + + E+ I++ HK IIGK G+N+ +DL N ++ R EA + I +E+ ++E L + + R + E++ P K +IG GG NI+K+R +T I FP D ++++TI+GT V+ + L I++++N + + + ++G+ L++I + + V ++FP SD ++L G ++ V+ +KK ++E I ++H++I+G +GI ++++T+ T K P + ++ D I+I+G+++ E DA+K + + I +E+ +LH +IG+ G+ I + + V I+ PSP+ +T+ I G ++ +A+ L + V E+ A D L+ + SV + K H +IIG+K V++ L R D +N+ +Q I+ G +N +SA+D I+HI+ D E ++E++ VD R I + +I +++V I+ P
Sbjct: 248 RFHRHLIGKSGAKINKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIE--QKFHRTIIGQKGERIREIREKFP-EVIINFPDPAQKS-DIVQLRGPKNEVEKCTKYLQKMVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANITELEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPSEGSGTNYAELKAKPEYHKFLIGKGGGNIRKVRDQTGARIIFPTADDKDQQLITIVGTEDSVKEAQKELEFLIKSLDNIVEDSMSVDPKHHRHFVIRRGQVLRDIAEEYGGVIVSFPRSGVQSDRVTLKGAKECVEAA----KKKILDIIED----LCLITQRFHRSIMGPKGIRVQQITREFGVQIKFPDREVTPQGGEILGTEVKATDPESPKKCDIILISGRKEKCEAASDALKALVPV--------SIEVEVPFDLHRYIIGQKGSGIRKMMDEF--EVNIQVPSPEQQCDTITITGQSANLDRAKAGLLERVRELQAEQ------------------EDRALRSFKLAVSV-----DPKFHPKIIGRKGAVIS-----------------------------------LIRSDHD----VNIQFPDKNDENQDQIIIT--GYERNTESARDAILHIVGDLEKMVSEDITVDHRVHARIIGARGKAIRKIMDEFKVDIRFP 913 HSP 4 Score: 80.8777 bits (198), Expect = 3.262e-20 Identity = 124/549 (22.59%), Postives = 232/549 (42.26%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRF--EDEANQ--IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSI-NFPRD-LTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFP--------------------PPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDA----IKRVCNIYXXXXXXXXXXXXXXXX----LHTILIGKGGNRINSLKESCGGRVTIRFPSP-DADSNTVIIKG-------VKDDVLKAQVKLHDLVEEITASNYEQHFQM 2080
K++H+ K + + E + ++D+P + + I I+G R A +L ++ A EL++ IP + H +IG +G I+ I+++ ++ +EGS N + A HKF+IGK G N+ + ++ ++ F D+ +Q I I G+ + V E + L+ + L + + + M +DP +H + G ++ I +E I +FPR + VT+ G VEA K +L IE + T ++G +G +++I F V+I FP P I LI RK+ + A+ A S +V++PF H+ IIG++G IR++ Q+P+ ++ D I ITG+ +++ A ++RV + ++ + +++ + H +IG+ G I+ ++ V I+FP D + + +II G +D +L L +V E ++ H ++
Sbjct: 389 KQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKEL-ANITELEVNIPSKLHNSLIGAKGRFIRSIMEECG---GVHIHFPSEGS-------------------------GTNYAELKAKPEYHKFLIGKGGGNIRKVR-DQTGARIIFPTADDKDQQLITIVGTEDSVKEAQKELEFLIKSLDN-IVEDSMSVDPKHHRHFVIRRGQVLRDIAEEYGGVIVSFPRSGVQSDRVTLKGAKECVEAAKKKILDIIEDLCL-------ITQRFHRSIMGPKGIRVQQITREF-GVQIKFPDREVTPQGGEILGTEVKATDPESPKKCDIILISGRKEKCEAAS-----DALKALVPVSIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSPEQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHPKIIGRKGAVISLIRSDHD--VNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSEDITVDHRVHARI 891 HSP 5 Score: 40.0466 bits (92), Expect = 3.262e-20 Identity = 27/95 (28.42%), Postives = 51/95 (53.68%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITRL---VQTQFEISLDIPKEFHRSLIGNKGSLHKEL-ENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVD 521
+ I G V +AKK + L ++ + L I ++FHR++IG KG +E+ E ++FP S +++++G K+ +E + +QK V+
Sbjct: 284 IRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYLQKMVE 378 HSP 6 Score: 93.5893 bits (231), Expect = 1.427e-18 Identity = 51/134 (38.06%), Postives = 77/134 (57.46%), Query Frame = 3
Query: 114 TFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
F +P EER+Y D + K+ +I G ++E ++ DQ L + +SG+ D V +A+K I +QTQ S+ IPKE HR +IG G ++LE T+T++ P+ + S IKI G+K+GIE AR I
Sbjct: 15 VFHVPLEERKYKDMAQFGEGEQAKICLDIMQKTGAQLELSLAKDQGLSIMVSGKHDSVMKARKEIVTRLQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGTKEGIEKARHEI 148 HSP 7 Score: 93.5893 bits (231), Expect = 1.609e-18 Identity = 70/265 (26.42%), Postives = 139/265 (52.45%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFV--LDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNF------SLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGI-IEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEE 3670
++G D + A++EI+ V +P+E + +IG GEK++ L I+IP ++ +TG+ E + A+ EI+ L +Q + I F ++ P+ HR +IG KG+ ++KI T+ ++ + + ++I+++G ++V A+ ++ A V L S + +NID R H LIG GA ++++K++YKV + P + E++N ++++G + V + EL++ A+ M +E + +E+
Sbjct: 65 VSGKHDSVMKARKEIVTRLQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPEEASNQIKITGTKEGIEKARHEIL-----LISAEQVKNICTFFPFLFTVASVSAPSWLHRFIIGKKGQNLAKI-TQQIPKVHIEFTEGE-DKISLEGPTEDVTVAQQQMEALVRDLISRMDFAEINIDHRFHRHLIGKSGAKINKIKEQYKVSVRIP---QDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQ 319 HSP 8 Score: 89.7373 bits (221), Expect = 2.151e-17 Identity = 71/260 (27.31%), Postives = 137/260 (52.69%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKL-------------FVLDL-EVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFT----KVIVTGSSESVVAAKKEIVEFVEEL-ERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
+++ITG K+ I A+ EI+ + F+ + V+ P + +IG KG+ + K+ T Q IP + +FT K+ + G +E V A++++ V +L RM +F+ +++ ++HR LIG G I+KI+ + V + +P N I I+G V++AK E+L +++E+ + L I+ + H +IG +G + ++++K+ +V I FP ++++IV+++G K V++C L
Sbjct: 131 QIKITGTKEGIEKARHEILLISAEQVKNICTFFPFLFTVASVSAPSWLHRFIIGKKGQNLAKI--TQQ----IPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALVRDLISRM-------DFA-EINIDHRFHRHLIGKSGAKINKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFP---DPAQKSDIVQLRGPKNEVEKCTKYL 373 HSP 9 Score: 89.7373 bits (221), Expect = 2.263e-17 Identity = 147/698 (21.06%), Postives = 275/698 (39.40%), Query Frame = 2
Query: 1667 GREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRIIGQK-ENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLI-VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSSTISAGVALN-KDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQ-----------------EREIRNF-----SLALHVPAQ--------------YHRKLIGNKGETISKIRTKNSVRISVPSQDSNINE-ITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIV-FPIRGTEGEEANIVKVKGTKESVDRCADELVD 3619
G + ++ + T + ++ A ++ I+++GK V A K I + + I K H +IGK G ++ L+ ++ I P P+ SN + I G K+ + KA+ EI + EQ + + FLF A + A H IIG+K +N+ + + K E F G D + GP +++ A+ ++ ++ D + + I +D R +++ S ++++I ++Y+V ++IP E N I + +K + N D++ E + G K I EI + I +N DP + Q + G K+++ + + K+ ++VP ++F+ +IG G IKK+R I +P +NS +++TG + AA+ I+ +EL + + R IR+ + +H P++ YH+ LIG G I K+R + RI P+ D + ITI G +D+V++A+ E+ + L++ + + +++D + H + RG L + ++Y IV FP G + + V +KG KE V+ +++D
Sbjct: 34 GEQAKICLDIMQKTGAQLELSLAKDQGLSIMVSGKHDSVMKARK---EIVTRLQTQASASVAIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPEEASNQIKITGTKEGIEKAR-------HEILLISAEQ-VKNICTFFPFLFTVASVS--------------APSWLHRFIIGKKGQNLAKITQQIPKVHIE-------------------------FTEGEDK--------------------ISLEGPTEDVTVAQQQMEALVRDLISRMDFAEINIDHRFHRHLIGKSGAKINKIKEQYKVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRME-------------NERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVI-----INFPDPAQKSDIVQLR----GPKNEVEKCTKYLQKMVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANITELEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPSEGSGTNYAELKAKPEYHKFLIGKGGGNIRKVRDQTGARIIFPTADDKDQQLITIVGTEDSVKEAQKELEFLIKSLDNIVEDSMSVDPKHHRHFVIRRGQVLRDIAEEYGGVIVSFPRSGVQSDR---VTLKGAKECVEAAKKKILD 634 HSP 10 Score: 73.9442 bits (180), Expect = 1.699e-12 Identity = 166/817 (20.32%), Postives = 334/817 (40.88%), Query Frame = 2
Query: 1439 LIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVK-----IPFKY-----------HKAIIGREGITIRELTKATNTKFQIPAA----SEENDEIVITGKQKDVEDAIKRV-CNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASN-----YEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSH----LRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQG---ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQ-VDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQD---------------------SNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPE 3709
+IG+ G+ L++++ + +I P P+ S+ I + G ++ +++ +H L+ S +++VK PF + H+ IIG++G + ++T+ QIP +E D+I + G +DV A +++ + + S + I I H LIGK G +IN +KE +V++R P SN + I+G V +A+ +L +L + EQ F T+ +++++RE + I D + +++ G K V + LQK +V + ++S+ F K+ + G N+ K+ P E ++ G N ++A+D I+ I + N ITE + +L N ++ + + + I F P + + + AKP ++ G G NI ++ + + I A +KD Q + I G +D + A++E+ L +++ ++V + + + +G+ ++ + + V + P + +V + G+ E V AAKK+I++ +E+L + Q ++HR ++G KG + +I + V+I P ++ + I I G K+ E A + + A V IEV + +H +IG +G+ + ++ +++V I P + ++ + + + G ++DR L++R + E DR + + + F P+
Sbjct: 101 VIGKNGEKLQDLELK-TATKIQIPRPEEASNQIKITGTKEGIEKA------RHEILLIS--AEQVKNICTFFPFLFTVASVSAPSWLHRFIIGKKGQNLAKITQ------QIPKVHIEFTEGEDKISLEGPTEDVTVAQQQMEALVRDLISRMDFAEINIDHRFHRHLIGKSGAKINKIKEQY--KVSVRIPQDSEKSNHIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTI------IGQKGERIREIREKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYLQK-------MVENSYSISVPIFKQFHKN--IIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELAN-ITELEVNIPSKLHNSLIGAKGRFIRSIMEECGGVHIHF---PSEGSGTNYAELKAKPEYHKFLIG-----------------------------KGGG----NIRKVRDQTGARIIFPTADDKD--------QQLITIVGTEDSVKEAQKELEFLIKSLDNIVEDSMSVDPKHHRHFVIRRGQVLRDIAEEYGGVIVSFPRSGVQSDRVTLKGAKECVEAAKKKILDIIEDLCLITQ---------------RFHRSIMGPKGIRVQQITREFGVQIKFPDREVTPQGGEILGTEVKATDPESPKKCDIILISGRKEKCEAASDALKALV----PVSIEV-EVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVP---SPEQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHPK 817 HSP 11 Score: 65.0846 bits (157), Expect = 9.036e-10 Identity = 66/258 (25.58%), Postives = 120/258 (46.51%), Query Frame = 2
Query: 647 PFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNI-----------IAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDE----ANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLT--KKVVTIIGTPHGV-EAVKAIL 1366
P S K +I + R +AAS L+ + +++E+P + H+YIIG +GSGI++++D+ V + + E +TI + G NL A + + + +A+ H IIG+ G+ ++ L + ++ ++F D+ +QI+I G + + V DL+ +S+ ++ +D + IIG+ G I+KI E I FP+ +VT+ G P V EA+ +L
Sbjct: 693 PESPKKCDIILISGRKEKCEAASDALKALVPVS-----IEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPSP---EQQCDTITITGQSANLDRAKAGLLERVRELQAEQEDRALRSFKLAVSVDPKFHPKIIGRKGA-VISLIRSDHDVNIQFPDKNDENQDQIIITGYERNTESARDAILHIVGDLEKMVSE-DITVDHRVHARIIGARGKAIRKIMDEFKVDIRFPQTGAPDPNLVTVTGKPEDVDEAIDHLL 940 HSP 12 Score: 55.0694 bits (131), Expect = 1.096e-6 Identity = 37/125 (29.60%), Postives = 64/125 (51.20%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKE 623
V + G K++V + KY+ ++V+ + IS+ I K+FH+++IG G+ K++ +T++ P N S +I I G + E AR I E + I V I S + N L+ R ++E
Sbjct: 357 VQLRGPKNEVEKCTKYLQKMVENSYSISVPIFKQFHKNIIGKGGANIKKIREECNTKIDLPAENSNSEMIVITGKRPNCEAARDRILAIQKELANITELEVNIPSKLHNSLIGAKGRFIRSIMEE 481
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Mouse
Match: Hdlbp (high density lipoprotein (HDL) binding protein [Source:MGI Symbol;Acc:MGI:99256]) HSP 1 Score: 368.622 bits (945), Expect = 4.267e-125 Identity = 292/989 (29.52%), Postives = 504/989 (50.96%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQ-KSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPE-PVNSKTISNSHVSAKPGE-----------------------KPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEV------SSTIS-AGVALNKDPISNIKLPQYKLEITGHKDKIT--LAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNK---NSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQY--HRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVL 3391
K +H N + +++ R++IPP SV++ EI +G+++ +AQA + + + Y + K + +E+ K +HKY+IGP+G+ ++EIL++T V V+I ++ T+ L G + LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V E ++ V DL +R+ +E+ ID +H+IG G NI +I+ + S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+D+FPEV INFP P SD++ L GP+ +V++ +QK A LVE+++S V I ++HK IIG+ G I+++ + +NTK +PA + ++ I+ITGK+ + E A R+ +I + +NI E+ + I LH LIG G I S+ E CGG V I FP + S+TV+I+G DV KA+ +L L EE ++ + E ++FL K++K+R++ RI +E +D+ + IIG+++ V + L+ + +V + + + +F + ++ R + + V +S + ++ + +G +++AK I II+D E +T + ++ +++ +++ +I++ Y VQI+ P E PV+S S + GE K + P L I+ + S K + E+N V S TI+ G+A N D G D++ A++E L L V V +++P +IG KG I ++R H+V+I+ P+K N ++ +TG ++ AA+ I++ V ELE+M E VP + H ++IG +G+ I KI + V I P S + N +T+ G +NVE+A + +L
Sbjct: 231 KAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPP---SDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGG-VHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLENVVEDYMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKDCVEAAKKRIQEIIEDLEAQVTVECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSDTIAITGLAANLDRAK-----------AGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSE----------DVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHIL 1191 HSP 2 Score: 102.064 bits (253), Expect = 4.267e-125 Identity = 54/134 (40.30%), Postives = 79/134 (58.96%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHAR 497
IT F +P EER+Y D + K+ EI G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G ++LE T+T++ P+ +DPS IKI G+K+GIE AR
Sbjct: 76 ITQVFHVPLEERKYKDMNQFGEGEQAKICLEIMQRTGAHLELSLAKDQGLSIMVSGKLDAVMKARKDIVARLQTQASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKAR 209 HSP 3 Score: 155.992 bits (393), Expect = 1.073e-37 Identity = 96/311 (30.87%), Postives = 174/311 (55.95%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN---INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQ 3826
I+G K+K AKE + L + +EV VP + + +IG KG I+K+ +V+I +P + + +TG + ++ AK +++ V+EL+ +++R +R+F L++ V +YH K+IG KG I++IR ++ V I P +D ++ITI G++ N E A++ +L V +LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N V V G E+V+ D +++ L ++ D L+ Y EE+ P+K GF +R+APW + ++ S+S++
Sbjct: 953 ISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSG--APDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQ-----VYMKPPAHEESRAPSK-------GFVVRDAPWTSNSSEKAPDMSSSEE 1249 HSP 4 Score: 93.2041 bits (230), Expect = 3.876e-27 Identity = 118/513 (23.00%), Postives = 217/513 (42.30%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESV--NIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQ---IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP------------------------RDLTKK---------VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQV-AAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEEN---DEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDA-DSNTVIIKGVKDDVLKAQVKLHDLVEEITA 2053
+ I G V +A LL + + + I E HK++IG G I+++ D T + + + I ++G +D + A L + E+V + + +D P F+I + G L +++ + + F Q + ++G+ + V + +++ + DL+++++ +E I + ++G G IQ+I ++ N I FP R+ + ++ I G EA K L ++ L +IGQ+G G++++ D F EV I+ P P+ SD I++ G ++D+ A +L + E +F V + KYH IIGR+G I ++ Q P + N D+I ITG +K+ E A + I + ++ + + +H +IG G I + + +V IRFP A D N V + G+ ++V +A + +L EE A
Sbjct: 700 VVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFPA--AEDKDQDLITIIGKEDAVREAQKELEALIQNLENVVEDYMLVD-PKHHRHFVI-RRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-VECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEAL-----EALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEF-EVNIHVPAPELQSDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF--KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLA 1199 HSP 5 Score: 50.0618 bits (118), Expect = 3.876e-27 Identity = 39/139 (28.06%), Postives = 69/139 (49.64%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITR----LVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIFLHF 653
V + G K++V + KY+ + LV+ + IS+ I K+FH+++IG G+ K++ ++T++ P N S I I G + E AR I + + I V I + + N L+ RL ++E + +HF
Sbjct: 554 VQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEE--CGGVHIHF 690 HSP 6 Score: 114.39 bits (285), Expect = 6.592e-25 Identity = 153/691 (22.14%), Postives = 298/691 (43.13%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXE-QGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLI-VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSSTISAGVALN-KDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDL-----EVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
+++ S V IP ++H+ +IG+ G +++L T TK QIP + +++I ITG ++ +E A V I ++ +E+ K H + G + + + G R+ I P P + ++ G K+ + +A ++ + EE ++ ++++ L+++ R SV I ++ S I+ G+ E + L EV KA S S++ ++ + F IG+ N+ K+ K E + + GP +++ A+++I ++ D N + I +D + +++ S ++ I +Y+V ++IP E N I + ++ + N D++ E + G K I EI + I +N DP + Q + G K+++ + + K+ V DL ++VP ++F+ +IG G IKK+R I +P +NS +I+TG + AA+ I+ ++L + + + + +PA+ H LIG KG I I + V I P + S + + I+G +VEKAK ++L + ++ V + H LIG G + +++ I+FP E ++ +++ + G +++V EL
Sbjct: 147 LQTQASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINI--PPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANS--------FTVSSVSAPSWLHR----FIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRME-------------NERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVI-----INFPDPAQKSDIVQLR----GPKNEVEKCTKYMQKM-VADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAE--------VEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFP--AAEDKDQDLITIIGKEDAVREAQKEL 790 HSP 7 Score: 110.538 bits (275), Expect = 1.048e-23 Identity = 141/643 (21.93%), Postives = 286/643 (44.48%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK-SNELF----RIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDER 2521
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++++ +T+ + I R ++ S N I + G K+ + A + L+ + + + ++ H FI G ++G+ N+ SVN+ +IV G E++ + +KK + + + + + +E+ K++IG G ++Q+I + T S+ P D + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP +DV+ ++ L+ ++ I K+H+ +IG+ G I + +IP SE+++ I I G + V+ A + + + + N + I + H +IG+ G RI +++ V I FP P S+ V ++G K++V K + +V ++ ++Y + + ++ + +KK+RE + +I +E + + I G++ N + ARS + I + ++ +++ K N L R+ R M + + + + RGP +++ AK +++H+ ++ + T++ VD R
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQTQASAT-----VPIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPDDPS-NQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR-----------TEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVSAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKF-PEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANC-------EAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQ---TKSFTVDIR 733 HSP 8 Score: 83.1889 bits (204), Expect = 2.108e-15 Identity = 65/251 (25.90%), Postives = 134/251 (53.39%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVL-----DLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRA 3625
+ I G + AK E+++L ++ + + F+ +IG KGE+I+++R +V I P+ K V + G K E+ + + +++M + ++S+++ + Q+H+ +IG G I KIR +++ +I +P+++SN I I G + N E A++ +L+ L + I EV ++I +++H+ LIG++G + + ++ V I FP+ EG ++ V ++G V++ +L+ A
Sbjct: 481 IRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGP-------KNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDL-ANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPV---EGSGSDTVVIRGPSSDVEKAKKQLLHLA 720 HSP 9 Score: 76.2554 bits (186), Expect = 2.980e-13 Identity = 98/450 (21.78%), Postives = 196/450 (43.56%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNE--------------------------GSP---NTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR----DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDA-DSDLISLIGPRKDVDQ-VAAILQKKHAFLVESNFSKKVKI---PFKYHKAIIGREGITIRELTKATNTKFQIP--AASEE 1744
K + + E + + P +++++ G +D V A + + ++A ++ IP++ H+ ++GP+GS I++I +V + ++ N GSP + I + G K+ A + ++ I ++ P LH++IIG+ GS + V++ V P +Q ++ I I G + L +V +LQ+ R +L + +DP IIG G I +IR E +I FP + + +TI G EA + +LK + +E +D+ H + ++IG +GK +++I D F +V I FP A D + +++ G ++V++ + IL + +L + S+ +++ P + ++ +G +R+ +N+ + P ++SEE
Sbjct: 810 KHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-VECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEAL--EALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQ------SDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF-KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQVYMKPPAHEESRAPSKGFVVRDAPWTSNSSEKAPDMSSSEE 1249 HSP 10 Score: 76.2554 bits (186), Expect = 3.109e-13 Identity = 64/287 (22.30%), Postives = 129/287 (44.95%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLFVLDLEVNV------PEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELV 3616
K+ + G KD + AK+ I ++ + DLE V P++F+ +++G KG +I+++ + V IK P++ + +I++G E AAK+ + V ++ + VP HR +IG KG I K+ + V I VP+ + + I I G N+++AK +L V +L++ + +D + H ++IG +GA + Q++ +++V I FP + + + + + G +++ + D ++
Sbjct: 846 KVTLKGAKDCVEAAKKRIQEI-IEDLEAQVTVECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEALEALVP-------------VTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAIL 1118 HSP 11 Score: 61.6178 bits (148), Expect = 7.494e-9 Identity = 34/124 (27.42%), Postives = 65/124 (52.42%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIG 3469
++V+G ++V+ A+K+IV ++ S + +P ++HR +IG GE + + K + +I +P D N+I I G K+ +EKA++EVL + + +E L ++ H + G
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQ-----------TQASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAG 239
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Mouse
Match: Hdlbp (high density lipoprotein (HDL) binding protein [Source:MGI Symbol;Acc:MGI:99256]) HSP 1 Score: 368.622 bits (945), Expect = 4.267e-125 Identity = 292/989 (29.52%), Postives = 504/989 (50.96%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQ-KSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPE-PVNSKTISNSHVSAKPGE-----------------------KPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEV------SSTIS-AGVALNKDPISNIKLPQYKLEITGHKDKIT--LAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNK---NSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQY--HRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVL 3391
K +H N + +++ R++IPP SV++ EI +G+++ +AQA + + + Y + K + +E+ K +HKY+IGP+G+ ++EIL++T V V+I ++ T+ L G + LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V E ++ V DL +R+ +E+ ID +H+IG G NI +I+ + S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+D+FPEV INFP P SD++ L GP+ +V++ +QK A LVE+++S V I ++HK IIG+ G I+++ + +NTK +PA + ++ I+ITGK+ + E A R+ +I + +NI E+ + I LH LIG G I S+ E CGG V I FP + S+TV+I+G DV KA+ +L L EE ++ + E ++FL K++K+R++ RI +E +D+ + IIG+++ V + L+ + +V + + + +F + ++ R + + V +S + ++ + +G +++AK I II+D E +T + ++ +++ +++ +I++ Y VQI+ P E PV+S S + GE K + P L I+ + S K + E+N V S TI+ G+A N D G D++ A++E L L V V +++P +IG KG I ++R H+V+I+ P+K N ++ +TG ++ AA+ I++ V ELE+M E VP + H ++IG +G+ I KI + V I P S + N +T+ G +NVE+A + +L
Sbjct: 231 KAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPP---SDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGG-VHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLENVVEDYMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKDCVEAAKKRIQEIIEDLEAQVTVECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSDTIAITGLAANLDRAK-----------AGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSE----------DVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHIL 1191 HSP 2 Score: 102.064 bits (253), Expect = 4.267e-125 Identity = 54/134 (40.30%), Postives = 79/134 (58.96%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHAR 497
IT F +P EER+Y D + K+ EI G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G ++LE T+T++ P+ +DPS IKI G+K+GIE AR
Sbjct: 76 ITQVFHVPLEERKYKDMNQFGEGEQAKICLEIMQRTGAHLELSLAKDQGLSIMVSGKLDAVMKARKDIVARLQTQASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKAR 209 HSP 3 Score: 155.992 bits (393), Expect = 1.073e-37 Identity = 96/311 (30.87%), Postives = 174/311 (55.95%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN---INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQ 3826
I+G K+K AKE + L + +EV VP + + +IG KG I+K+ +V+I +P + + +TG + ++ AK +++ V+EL+ +++R +R+F L++ V +YH K+IG KG I++IR ++ V I P +D ++ITI G++ N E A++ +L V +LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N V V G E+V+ D +++ L ++ D L+ Y EE+ P+K GF +R+APW + ++ S+S++
Sbjct: 953 ISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSG--APDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQ-----VYMKPPAHEESRAPSK-------GFVVRDAPWTSNSSEKAPDMSSSEE 1249 HSP 4 Score: 93.2041 bits (230), Expect = 3.876e-27 Identity = 118/513 (23.00%), Postives = 217/513 (42.30%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESV--NIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQ---IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP------------------------RDLTKK---------VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQV-AAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEEN---DEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDA-DSNTVIIKGVKDDVLKAQVKLHDLVEEITA 2053
+ I G V +A LL + + + I E HK++IG G I+++ D T + + + I ++G +D + A L + E+V + + +D P F+I + G L +++ + + F Q + ++G+ + V + +++ + DL+++++ +E I + ++G G IQ+I ++ N I FP R+ + ++ I G EA K L ++ L +IGQ+G G++++ D F EV I+ P P+ SD I++ G ++D+ A +L + E +F V + KYH IIGR+G I ++ Q P + N D+I ITG +K+ E A + I + ++ + + +H +IG G I + + +V IRFP A D N V + G+ ++V +A + +L EE A
Sbjct: 700 VVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFPA--AEDKDQDLITIIGKEDAVREAQKELEALIQNLENVVEDYMLVD-PKHHRHFVI-RRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-VECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEAL-----EALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEF-EVNIHVPAPELQSDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF--KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLA 1199 HSP 5 Score: 50.0618 bits (118), Expect = 3.876e-27 Identity = 39/139 (28.06%), Postives = 69/139 (49.64%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITR----LVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIFLHF 653
V + G K++V + KY+ + LV+ + IS+ I K+FH+++IG G+ K++ ++T++ P N S I I G + E AR I + + I V I + + N L+ RL ++E + +HF
Sbjct: 554 VQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEE--CGGVHIHF 690 HSP 6 Score: 114.39 bits (285), Expect = 6.592e-25 Identity = 153/691 (22.14%), Postives = 298/691 (43.13%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXE-QGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLI-VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSSTISAGVALN-KDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDL-----EVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
+++ S V IP ++H+ +IG+ G +++L T TK QIP + +++I ITG ++ +E A V I ++ +E+ K H + G + + + G R+ I P P + ++ G K+ + +A ++ + EE ++ ++++ L+++ R SV I ++ S I+ G+ E + L EV KA S S++ ++ + F IG+ N+ K+ K E + + GP +++ A+++I ++ D N + I +D + +++ S ++ I +Y+V ++IP E N I + ++ + N D++ E + G K I EI + I +N DP + Q + G K+++ + + K+ V DL ++VP ++F+ +IG G IKK+R I +P +NS +I+TG + AA+ I+ ++L + + + + +PA+ H LIG KG I I + V I P + S + + I+G +VEKAK ++L + ++ V + H LIG G + +++ I+FP E ++ +++ + G +++V EL
Sbjct: 147 LQTQASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINI--PPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANS--------FTVSSVSAPSWLHR----FIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRME-------------NERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVI-----INFPDPAQKSDIVQLR----GPKNEVEKCTKYMQKM-VADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAE--------VEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFP--AAEDKDQDLITIIGKEDAVREAQKEL 790 HSP 7 Score: 110.538 bits (275), Expect = 1.048e-23 Identity = 141/643 (21.93%), Postives = 286/643 (44.48%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK-SNELF----RIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDER 2521
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++++ +T+ + I R ++ S N I + G K+ + A + L+ + + + ++ H FI G ++G+ N+ SVN+ +IV G E++ + +KK + + + + + +E+ K++IG G ++Q+I + T S+ P D + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP +DV+ ++ L+ ++ I K+H+ +IG+ G I + +IP SE+++ I I G + V+ A + + + + N + I + H +IG+ G RI +++ V I FP P S+ V ++G K++V K + +V ++ ++Y + + ++ + +KK+RE + +I +E + + I G++ N + ARS + I + ++ +++ K N L R+ R M + + + + RGP +++ AK +++H+ ++ + T++ VD R
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQTQASAT-----VPIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPDDPS-NQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR-----------TEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVSAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKF-PEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANC-------EAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQ---TKSFTVDIR 733 HSP 8 Score: 83.1889 bits (204), Expect = 2.108e-15 Identity = 65/251 (25.90%), Postives = 134/251 (53.39%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVL-----DLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRA 3625
+ I G + AK E+++L ++ + + F+ +IG KGE+I+++R +V I P+ K V + G K E+ + + +++M + ++S+++ + Q+H+ +IG G I KIR +++ +I +P+++SN I I G + N E A++ +L+ L + I EV ++I +++H+ LIG++G + + ++ V I FP+ EG ++ V ++G V++ +L+ A
Sbjct: 481 IRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGP-------KNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDL-ANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPV---EGSGSDTVVIRGPSSDVEKAKKQLLHLA 720 HSP 9 Score: 76.2554 bits (186), Expect = 2.980e-13 Identity = 98/450 (21.78%), Postives = 196/450 (43.56%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNE--------------------------GSP---NTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR----DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDA-DSDLISLIGPRKDVDQ-VAAILQKKHAFLVESNFSKKVKI---PFKYHKAIIGREGITIRELTKATNTKFQIP--AASEE 1744
K + + E + + P +++++ G +D V A + + ++A ++ IP++ H+ ++GP+GS I++I +V + ++ N GSP + I + G K+ A + ++ I ++ P LH++IIG+ GS + V++ V P +Q ++ I I G + L +V +LQ+ R +L + +DP IIG G I +IR E +I FP + + +TI G EA + +LK + +E +D+ H + ++IG +GK +++I D F +V I FP A D + +++ G ++V++ + IL + +L + S+ +++ P + ++ +G +R+ +N+ + P ++SEE
Sbjct: 810 KHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-VECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEAL--EALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQ------SDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF-KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQVYMKPPAHEESRAPSKGFVVRDAPWTSNSSEKAPDMSSSEE 1249 HSP 10 Score: 76.2554 bits (186), Expect = 3.109e-13 Identity = 64/287 (22.30%), Postives = 129/287 (44.95%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLFVLDLEVNV------PEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELV 3616
K+ + G KD + AK+ I ++ + DLE V P++F+ +++G KG +I+++ + V IK P++ + +I++G E AAK+ + V ++ + VP HR +IG KG I K+ + V I VP+ + + I I G N+++AK +L V +L++ + +D + H ++IG +GA + Q++ +++V I FP + + + + + G +++ + D ++
Sbjct: 846 KVTLKGAKDCVEAAKKRIQEI-IEDLEAQVTVECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEALEALVP-------------VTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAIL 1118 HSP 11 Score: 61.6178 bits (148), Expect = 7.494e-9 Identity = 34/124 (27.42%), Postives = 65/124 (52.42%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIG 3469
++V+G ++V+ A+K+IV ++ S + +P ++HR +IG GE + + K + +I +P D N+I I G K+ +EKA++EVL + + +E L ++ H + G
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQ-----------TQASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAG 239
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Mouse
Match: Hdlbp (high density lipoprotein (HDL) binding protein [Source:MGI Symbol;Acc:MGI:99256]) HSP 1 Score: 331.643 bits (849), Expect = 4.432e-94 Identity = 283/994 (28.47%), Postives = 489/994 (49.20%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAA-SILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFT----KSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQ-KSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPE-PVNSKTISNSHVSAKPGE-----------------------KPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEV------SSTIS-AGVALNKDPISNIKLPQYKLEITGHKDKIT--LAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNK---NSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQY--HRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVL 3391
K +H+ K+ L + + + ++ IP N+I I+G ++G+ +A +LL + Q K + L++E K H +I GP + EI+ +T ++I +N I G K+ L A+ + K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V E ++ V DL +R+ +E+ ID +H+IG G NI +I+ + S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+D+FPEV INFP P SD++ L GP+ +V++ +QK A LVE+++S V I ++HK IIG+ G I+++ + +NTK +PA + ++ I+ITGK+ + E A R+ +I + +NI E+ + I LH LIG G I S+ E CGG V I FP + S+TV+I+G DV KA+ +L L EE ++ + E ++FL K++K+R++ RI +E +D+ + IIG+++ V + L+ + +V + + + +F + ++ R + + V +S + ++ + +G +++AK I II+D E +T + ++ +++ +++ +I++ Y VQI+ P E PV+S S + GE K + P L I+ + S K + E+N V S TI+ G+A N D G D++ A++E L L V V +++P +IG KG I ++R H+V+I+ P+K N ++ +TG ++ AA+ I++ V ELE+M E VP + H ++IG +G+ I KI + V I P S + N +T+ G +NVE+A + +L
Sbjct: 159 KEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVE--KAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR---TEIVFTGEKEQLAQAVARIKKIYEEKANSFTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGG-VHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLENVVEDYMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKDCVEAAKKRIQEIIEDLEAQVTVECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSDTIAITGLAANLDRAK-----------AGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSE----------DVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHIL 1122 HSP 2 Score: 155.606 bits (392), Expect = 1.205e-37 Identity = 96/311 (30.87%), Postives = 174/311 (55.95%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN---INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQ 3826
I+G K+K AKE + L + +EV VP + + +IG KG I+K+ +V+I +P + + +TG + ++ AK +++ V+EL+ +++R +R+F L++ V +YH K+IG KG I++IR ++ V I P +D ++ITI G++ N E A++ +L V +LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N V V G E+V+ D +++ L ++ D L+ Y EE+ P+K GF +R+APW + ++ S+S++
Sbjct: 884 ISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSG--APDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQ-----VYMKPPAHEESRAPSK-------GFVVRDAPWTSNSSEKAPDMSSSEE 1180 HSP 3 Score: 136.732 bits (343), Expect = 7.258e-32 Identity = 164/688 (23.84%), Postives = 304/688 (44.19%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYS---VRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKS--NELFR--IGRD----NMCKLNVXXXXXXXXXXEQG-ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIPFYPEPVNSKTISNSHVSAKPGEK--PYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN--GSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVD 3595
+++ S V IP ++H+ +IG+ G +++L T TK QIP + +++I ITG ++ +E A V I ++ +E+ K H + G + + + G R+ I P P + ++ G K+ + +A ++ + EE S + F+ L K+ + + +E EDK L G E+V E ++ ++ IN + Y + + ++ R IG+ N K +E+ ++ G Q ++ AK E++ + EN T++LI+++R + I ++ EI K+ +V I +P+P I EK YM + L N + I + + N G NI +I E ++ I A N + + I ITG + A+ I+ + + ++EV++P + + +LIG KG I+ + V I P + S V++ G S V AKK+++ EE ++ ++F++ + +YH+ LIG G I K+R RI P ++D + + ITI G +D V +A+ E+ A + LE+ + + + +D + H + RG L ++ ++Y V + FP GT+ ++ V +KG K+ V+
Sbjct: 147 LQTQASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINI--PPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKANSFTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLE--GPTEDVNVAQEQIEGMVKDL-----------INRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIIN---FPDPAQKSDIVQLRGPKNEVEKCTKYMQKM---------VADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLP-AENSNSETII--------ITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEE-------KQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLENVVEDYMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKDCVE 788 HSP 4 Score: 93.2041 bits (230), Expect = 3.879e-27 Identity = 118/513 (23.00%), Postives = 217/513 (42.30%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESV--NIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQ---IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP------------------------RDLTKK---------VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQV-AAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEEN---DEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDA-DSNTVIIKGVKDDVLKAQVKLHDLVEEITA 2053
+ I G V +A LL + + + I E HK++IG G I+++ D T + + + I ++G +D + A L + E+V + + +D P F+I + G L +++ + + F Q + ++G+ + V + +++ + DL+++++ +E I + ++G G IQ+I ++ N I FP R+ + ++ I G EA K L ++ L +IGQ+G G++++ D F EV I+ P P+ SD I++ G ++D+ A +L + E +F V + KYH IIGR+G I ++ Q P + N D+I ITG +K+ E A + I + ++ + + +H +IG G I + + +V IRFP A D N V + G+ ++V +A + +L EE A
Sbjct: 631 VVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFPA--AEDKDQDLITIIGKEDAVREAQKELEALIQNLENVVEDYMLVD-PKHHRHFVI-RRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-VECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEAL-----EALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEF-EVNIHVPAPELQSDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF--KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLA 1130 HSP 5 Score: 50.0618 bits (118), Expect = 3.879e-27 Identity = 39/139 (28.06%), Postives = 69/139 (49.64%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITR----LVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIFLHF 653
V + G K++V + KY+ + LV+ + IS+ I K+FH+++IG G+ K++ ++T++ P N S I I G + E AR I + + I V I + + N L+ RL ++E + +HF
Sbjct: 485 VQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEE--CGGVHIHF 621 HSP 6 Score: 102.064 bits (253), Expect = 3.472e-21 Identity = 54/134 (40.30%), Postives = 79/134 (58.96%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHAR 497
IT F +P EER+Y D + K+ EI G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G ++LE T+T++ P+ +DPS IKI G+K+GIE AR
Sbjct: 76 ITQVFHVPLEERKYKDMNQFGEGEQAKICLEIMQRTGAHLELSLAKDQGLSIMVSGKLDAVMKARKDIVARLQTQASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKAR 209 HSP 7 Score: 84.7297 bits (208), Expect = 6.910e-16 Identity = 66/245 (26.94%), Postives = 127/245 (51.84%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLF------VLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFT----KVIVTGSSESVVAAKKEIVEFVEEL-ERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRC 3601
TG K+++ A I K++ V+ P + +IG KG+ + K+ T Q +P + +FT K+ + G +E V A+++I V++L RM + +++ ++HR LIG G I++I+ + V + +P N I I+G V++AK E+L +++E+ + L I+ R H +IG +G + +++ K+ +V I FP ++++IV+++G K V++C
Sbjct: 270 FTGEKEQLAQAVARIKKIYEEKANSFTVSSVSAPSWLHRFIIGKKGQNLAKI--TQQ----MPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMD--------YVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFP---DPAQKSDIVQLRGPKNEVEKC 497 HSP 8 Score: 83.1889 bits (204), Expect = 2.340e-15 Identity = 65/251 (25.90%), Postives = 134/251 (53.39%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVL-----DLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRA 3625
+ I G + AK E+++L ++ + + F+ +IG KGE+I+++R +V I P+ K V + G K E+ + + +++M + ++S+++ + Q+H+ +IG G I KIR +++ +I +P+++SN I I G + N E A++ +L+ L + I EV ++I +++H+ LIG++G + + ++ V I FP+ EG ++ V ++G V++ +L+ A
Sbjct: 412 IRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGP-------KNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDL-ANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPV---EGSGSDTVVIRGPSSDVEKAKKQLLHLA 651 HSP 9 Score: 81.6481 bits (200), Expect = 5.460e-15 Identity = 113/473 (23.89%), Postives = 210/473 (44.40%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKET-NCSINFPRDLTKKVVTIIGTPHGVE-AVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXX-XXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKL 2026
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++++ +T+ + I R ++ S N I + G K+ + A + L+ + + + ++ H FI G ++G+ N+ SVN+ +IV G E++ + +KK + + + + + + IIG G N+ KI ++ I F K +T+ G V A + I + I +I H LIG+ G + IKD++ +V + PP S+LI + G + V Q L + A +E+ +K + I ++H+ IIG++G IRE+ K P ++++D + + G + +VE K + + K H +IGKGG I ++E ++ + P+ +++S T+II G + + A+ ++
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQTQASAT-----VPIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPDDPS-NQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR-----------TEIVFTGEKEQLAQAVARIKKIYEEKANSFTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDK--ITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQY-KVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELL-ELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDL--PAENSNSETIIITGKRANCEAARSRI 574 HSP 10 Score: 76.2554 bits (186), Expect = 2.731e-13 Identity = 98/450 (21.78%), Postives = 196/450 (43.56%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNE--------------------------GSP---NTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR----DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDA-DSDLISLIGPRKDVDQ-VAAILQKKHAFLVESNFSKKVKI---PFKYHKAIIGREGITIRELTKATNTKFQIP--AASEE 1744
K + + E + + P +++++ G +D V A + + ++A ++ IP++ H+ ++GP+GS I++I +V + ++ N GSP + I + G K+ A + ++ I ++ P LH++IIG+ GS + V++ V P +Q ++ I I G + L +V +LQ+ R +L + +DP IIG G I +IR E +I FP + + +TI G EA + +LK + +E +D+ H + ++IG +GK +++I D F +V I FP A D + +++ G ++V++ + IL + +L + S+ +++ P + ++ +G +R+ +N+ + P ++SEE
Sbjct: 741 KHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-VECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEAL--EALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQ------SDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF-KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQVYMKPPAHEESRAPSKGFVVRDAPWTSNSSEKAPDMSSSEE 1180 HSP 11 Score: 75.8702 bits (185), Expect = 3.180e-13 Identity = 64/287 (22.30%), Postives = 129/287 (44.95%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLFVLDLEVNV------PEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELV 3616
K+ + G KD + AK+ I ++ + DLE V P++F+ +++G KG +I+++ + V IK P++ + +I++G E AAK+ + V ++ + VP HR +IG KG I K+ + V I VP+ + + I I G N+++AK +L V +L++ + +D + H ++IG +GA + Q++ +++V I FP + + + + + G +++ + D ++
Sbjct: 777 KVTLKGAKDCVEAAKKRIQEI-IEDLEAQVTVECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEALEALVP-------------VTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAIL 1049 HSP 12 Score: 68.9366 bits (167), Expect = 4.348e-11 Identity = 58/241 (24.07%), Postives = 113/241 (46.89%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLE--VNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLA-FVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
++G D + A+++I+ V +P+E + +IG GEK++ L I+IP + ++ +TG+ E + A+ E++ E ++ ER L V +H + G + +I + RI++P N EI G K+ + +A + + K S + ++ S +H +IG +G NL ++ ++ KV I F TEGE+ + ++G E V+ +++
Sbjct: 129 VSGKLDAVMKARKDIVARLQTQASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVER--------LEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKANSFTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEF----TEGEDK--ITLEGPTEDVNVAQEQI 355 HSP 13 Score: 61.6178 bits (148), Expect = 7.388e-9 Identity = 34/124 (27.42%), Postives = 65/124 (52.42%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIG 3469
++V+G ++V+ A+K+IV ++ S + +P ++HR +IG GE + + K + +I +P D N+I I G K+ +EKA++EVL + + +E L ++ H + G
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQT-----------QASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAG 239
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Mouse
Match: Gm382 (predicted gene 382 [Source:MGI Symbol;Acc:MGI:2685228]) HSP 1 Score: 201.445 bits (511), Expect = 8.627e-52 Identity = 219/840 (26.07%), Postives = 393/840 (46.79%), Query Frame = 2
Query: 980 AIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSIN-FPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPV-----------------NSKTISNSHVSAKPGEKPYMNG-VDXXXXXXXXXXXXXP------NPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLP----QYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFT--KVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVL 3391
+I AP WLH+FIIG G + +++ P +Q+ F + ++I ++G ++V E V DL S++ E+ D K++ G+ G + +I ++ SI FP + + + I G GV K LL +E +DII H LIGQ+G+ ++EI +FP+V +NFP P SD++ LIGPR + ++ A L+ + E+N+S V I K HK IIG+ IR++++ATNTK P S ++E +ITG ++ E A + ++ ++ ++ E I I NL+ L +NS+ E C G++ + FP ++ N +II G ++V KA+ KL L EE A NY + + + ++FL + K+ + + +D+ + I G +E+V + L + V +++ ++ YF + +L + ++ + + S + I RG +++AK I I + + IT ++ I +++ +I++ Y+V+I+ P +P +K I+ + S + G+ +++G V++ + E+ S+ P P HL I+ S K E++ +VS N D IS + L Q K+++ + + E+ L L N+ ++ + G KG I ++ T H V I P K + ++ +TG E+ +AA+ I+ + ++E + S + + Q +IG +G+TI+KI + V I +P + IT+ G DNVEKA +L
Sbjct: 356 SIFAPSWLHRFIIGTKGEEISEITECVPKVQIHFTAK-DKITLKGPIDDVNYAQEKFDIIVKDLMSKMEYTEINSDSKFYKYLTGNNGEILNRITEKNQVSITMFPENESNYSIRIEGESLGVHQAKKELLDLANNLEEEYSQDIIIKHQFHHILIGQKGERVREICKKFPDVILNFPHPAEKSDIVQLIGPRYESEKCAQYLENMLTDIKENNYSISVPIIKKLHKRIIGKGVSNIRKISEATNTKITFPPESCNSEEFIITGYPENCEIARNWILSLQQELADTAEEEIIIPANLYKHLTNPKECLLNSIIEEC-GKIHLHFPKGKSNLNKIIIMGTIENVEKAKTKLLKLSEEEQAKNYSETLHIKSKYHQFLLNKNGGNISKICDETGTCVFFPNPTNKDQETITITGTEESVKEVQKQLDDLVKDFENEVDDSILINRRFHHYFVMRRGQLLKEMAEDYGGVVITFSYSGRQNTKVTI---RGAKPCVEAAKKHIKEIFEPLGSQITTRYVLPHSFQPFIMGPISSRIQQIARDYKVEIKFPDIEKPALNMDLGTHEKGKEKWKRTAKEIAPN--SPRKGDTIFISGQVENCKAATEALASIIPVTTEVHVPLHLQPYIIGHKGSGLRKLVKEYEVHMQVSQP-----GKNSDIISIMGLSANVEQAKIKLQKRVKSLQMEVEDRT-LRNFKLMFNLDPKYQAKITGHKGLLITQICTEHDVTIHFPKKGTHDMQEQITITGYKENTLAARDAIMRLLHKIE--------KTISKEITLNQQVRGNVIGVRGKTINKIMDQYQVDIRLPPKGLYNPNITVTGLADNVEKAIEHIL 1174 HSP 2 Score: 107.457 bits (267), Expect = 8.099e-23 Identity = 72/264 (27.27%), Postives = 144/264 (54.55%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKI--PNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN--INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLD-EINDRMQLEENKS 3679
I+G + A E + + + EV+VP P +IG KG ++KL ++V +++ P KNS ++ G S +V AK ++ + V+ L+ ++R +RNF L ++ +Y K+ G+KG I++I T++ V I P + ++ +ITI G+K+N A++ ++ ++K+E I + + ++ ++ +IG RG ++++ +Y+V+I P +G + V G ++V++ + +++ L IN Q E+ KS
Sbjct: 938 ISGQVENCKAATEALASIIPVTTEVHVPLHLQPYIIGHKGSGLRKLVKEYEVHMQVSQPGKNSDIISIM--GLSANVEQAKIKLQKRVKSLQMEVEDRTLRNFKLMFNLDPKYQAKITGHKGLLITQICTEHDVTIHFPKKGTHDMQEQITITGYKENTLAARDAIMRLLHKIEKTISKEITLNQQVRGNVIGVRGKTINKIMDQYQVDIRLPPKGLYNPN---ITVTGLADNVEKAIEHILNLEKYYLSAAINHGSQQEQPKS 1196 HSP 3 Score: 71.2478 bits (173), Expect = 8.677e-12 Identity = 57/239 (23.85%), Postives = 114/239 (47.70%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKL-RTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINE-ITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVD 3595
ITG+ + +A+ I+ L + E+ +P Y L K + + ++ + P S K+I+ G+ E+V AK +++ ++ +E + +N+S LH+ ++YH+ L+ G ISKI + + P+ + E ITI G +++V++ + ++ V E+ + + + I+ R H + RG L ++ + Y V I F G + + V ++G K V+
Sbjct: 616 ITGYPENCEIARNWILSLQQELADTAEEEIIIPANLYKHLTNPKECLLNSIIEECGKIHLHFPKGKSNLNKIIIMGTIENVEKAKTKLL-------KLSEEEQAKNYSETLHIKSKYHQFLLNKNGGNISKICDETGTCVFFPNPTNKDQETITITGTEESVKEVQKQLDDLVKDFENEVDDSILINRRFHHYFVMRRGQLLKEMAEDYGGVVITFSYSGRQNTK---VTIRGAKPCVE 844 HSP 4 Score: 68.1662 bits (165), Expect = 7.131e-11 Identity = 61/216 (28.24%), Postives = 108/216 (50.00%), Query Frame = 2
Query: 2981 VPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFT---KVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISV-PSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
P + +IG KGE+I ++ +P FT K+ + G + V A+++ V++L + EI + S ++++ L GN GE +++I KN V I++ P +SN + I I+G V +AK E+L N LE + + I + H LIG +G + ++ KK+ V + FP E+++IV++ G + ++CA L
Sbjct: 359 APSWLHRFIIGTKGEEISEITEC------VPKVQIHFTAKDKITLKGPIDDVNYAQEKFDIIVKDLMSKMEYTEINSDS-------KFYKYLTGNNGEILNRITEKNQVSITMFPENESNYS-IRIEGESLGVHQAKKELLDLANNLEEEYSQDIIIKHQFHHILIGQKGERVREICKKFPDVILNFP---HPAEKSDIVQLIGPRYESEKCAQYL 557 HSP 5 Score: 65.0846 bits (157), Expect = 7.810e-10 Identity = 73/307 (23.78%), Postives = 140/307 (45.60%), Query Frame = 2
Query: 2756 DIMS-----EHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQ----YKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEE----------FYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELV 3616
D+MS E NS+ Y ++ N E+ + I+ K+ +S P+ Y + I G + AK+E+ LDL N+ EE F+ LIG KGE+++ ++ K P+ F ++ + E + + LE M + + N+S+++ + + H+++IG I KI + +I+ P + N E I G+ +N E A+N +L+ +L E + I + ++ L + L+ + ++ K+ + FP +G N + + GT E+V++ +L+
Sbjct: 417 DLMSKMEYTEINSDSKFYKYLTGNNGEILNRITE-----KNQVSITMFPENESNYSIRIEGESLGVHQAKKEL-----LDLANNLEEEYSQDIIIKHQFHHILIGQKGERVR------EICKKFPDVILNFPHPAEKSDIVQLIGPRYESEKCAQYLENMLTDIKENNYSISVPIIKKLHKRIIGKGVSNIRKISEATNTKITFPPESCNSEEFIITGYPENCEIARNWILSLQQELADTAEEEIIIPANLYKHLTNPKECLLNSIIEECGKIHLHFP-KGKSN--LNKIIIMGTIENVEKAKTKLL 704 HSP 6 Score: 59.3066 bits (142), Expect = 4.017e-8 Identity = 104/475 (21.89%), Postives = 208/475 (43.79%), Query Frame = 2
Query: 776 PKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPAL----TLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLS-VNKPNIQLRFEDEAN-QIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKH-----IIGSGGVNIQKI-RKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXX-FTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIG-REGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXX-XXXLHTILIGKGGNRINSLKESCGGRVTIRFPSP-DADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETY 2149
P H++IIG +G I EI + + + +++ + + I L GP D++ A +V + I++ +K++ G +G L ++ N+ +I + E+E+N I IEG V + KK++ DL + L + E D ++IKH +IG G +++I +K + +NFP K +V +IG + E L + I+ + L ++IG+ +++I + +I FPP +S+ + G ++ + + L ++ +++ IP +K + +E + + + P ++I+I G ++VE A ++ + E+E H L+ K G I+ + + G + FP+P + D T+ I G ++ V + Q +L DLV++ + + + + + M LK++ E Y
Sbjct: 360 PSWLHRFIIGTKGEEISEITEC------VPKVQIHFTAKDKITLKGPIDDVNYAQEKFDIIVKDLMSKMEYTEINSDSKFYKYLTGNNGEILNRITEKNQVSITMFPENESNYSIRIEGESLGVHQA----KKELLDLANNLEE-EYSQD-IIIKHQFHHILIGQKGERVREICKKFPDVILNFPHPAEKSDIVQLIGPRYESEKCAQYLENMLTDIKENNYSISVPIIKKLHKRIIGKGVSNIRKISEA-TNTKITFPPESCNSEEFIITGYPENCEIARNWILSLQQELADTA-EEEIIIPANLYKHLTNPKECLLNSIIEECGKIHLHFPKGKSNLNKIIIMGTIENVEKAKTKLLKLSEEEQAKNYSETLHIKSKYHQFLLNKNGGNISKICDETG--TCVFFPNPTNKDQETITITGTEESVKEVQKQLDDLVKDF-ENEVDDSILINRRFHHYFVMRRGQLLKEMAEDY 817 HSP 7 Score: 52.7582 bits (125), Expect = 4.022e-6 Identity = 45/147 (30.61%), Postives = 78/147 (53.06%), Query Frame = 2
Query: 3212 ALHVPAQYHRKLIGNKGETISKI-RTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGI-IEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEI-VFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDE 3643
++ P+ HR +IG KGE IS+I V+I ++D +IT++G D+V A+ + V L S + +N DS+ + L G+ G L+++ +K +V I +FP E E ++++G V + EL+D AN + +E
Sbjct: 356 SIFAPSWLHRFIIGTKGEEISEITECVPKVQIHFTAKD----KITLKGPIDDVNYAQEKFDIIVKDLMSKMEYTEINSDSKFYKYLTGNNGEILNRITEKNQVSITMFP----ENESNYSIRIEGESLGVHQAKKELLDLANNLEEE 494
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Mouse
Match: 4921511C20Rik (RIKEN cDNA 4921511C20 gene [Source:MGI Symbol;Acc:MGI:2685229]) HSP 1 Score: 105.916 bits (263), Expect = 3.077e-23 Identity = 84/335 (25.07%), Postives = 155/335 (46.27%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN--INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIP-----MPSQTSSWR 3886
I+G + A E + L EV VP +P +IG KG IKK+ + V ++ S + + G + V AK ++ + + L+ ++R +RNF L + A+YH + G+KG I++I ++ V I P + + ++ITI G+K N AK+ ++ ++K+E + + + I+ ++ + G G + ++ +++ V+I P +G+ ++V G ++V D + D L + NY+S E K+ +K + +P ++V NDG S+ S N N + P PS+T WR
Sbjct: 158 ISGQIENCKAAMEALEYLIPFTAEVQVPSHLHPYIIGPKGSGIKKIMSEFDVHTQVSQTGSNSDIISIMGLAAKVKQAKVKLEKQINALQTEIEDRALRNFKLMFTLDAKYHSTITGHKGLFIAQICREHDVTIHFPKKANREIQDQITITGYKKNTLAAKDAIMRMLHKIEKTVSKEIPINPQVRDSIFGFGGKFIHKMNEQFHVDIRLPPKGSWNTNVSVV---GLPDNVQEAIDHIFDLEKYYLSAL-----------INYDSQL------ESLKNTCLYKITME---SPNNSVKNDGP-HSAKSYPNSSNDDSEDFPKLKQQTPSKTHPWR 468 HSP 2 Score: 64.3142 bits (155), Expect = 6.757e-10 Identity = 63/271 (23.25%), Postives = 121/271 (44.65%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFT---KVIVTGSSESVVAAKKEIVE----------FVEELERMKQERE----IRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIE--------VLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELV 3616
++ + G K + AK+ I +F + + +P++FY +G K++++ +V + P+K T VI E + KEIV ++E K E + F+ + VP+ H +IG KG I KI ++ V V SN + I+I G V++AK ++ +N L++ I + + +D++ HS + G +G + Q+ +++ V I FP + E + + + G K++ D ++
Sbjct: 53 RVTMKGEKACVEAAKKHIQHIFEPLGSHVTTQYVIPQKFYTFFMGPLCSKVQQIARDFKVQLAFPDKEKPTTYIHPVIQEAEKEKWGKSTKEIVFSNQRKNKTILISGQIENCKAAMEALEYLIPFTAEVQVPSHLHPYIIGPKGSGIKKIMSEFDVHTQVSQTGSNSDIISIMGLAAKVKQAKVKLEKQINALQTEIEDRALRNFKLMFTLDAKYHSTITGHKGLFIAQICREHDVTIHFPKKANR-EIQDQITITGYKKNTLAAKDAIM 322 HSP 3 Score: 63.5438 bits (153), Expect = 1.177e-9 Identity = 59/212 (27.83%), Postives = 97/212 (45.75%), Query Frame = 2
Query: 1427 LMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKK-HAFLVE------SNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIP--AASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDL 2035
L P +IG +G G+K+I F +V ++SD+IS++G V Q L+K+ +A E NF + KYH I G +G+ I ++ + + P A E D+I ITG +K+ A + + K + I I+ + + G GG I+ + E V IR P + + V + G+ D+V +A + DL
Sbjct: 188 LHPYIIGPKGSGIKKIMSEF-DVHTQVSQTGSNSDIISIMGLAAKVKQAKVKLEKQINALQTEIEDRALRNFKLMFTLDAKYHSTITGHKGLFIAQICREHDVTIHFPKKANREIQDQITITGYKKNTLAAKDAIMRMLHKIEKTVSKEIPINPQVRDSIFGFGGKFIHKMNEQF--HVDIRLPPKGSWNTNVSVVGLPDNVQEAIDHIFDL 396 HSP 4 Score: 53.1434 bits (126), Expect = 1.924e-6 Identity = 55/222 (24.77%), Postives = 102/222 (45.95%), Query Frame = 2
Query: 764 QIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGS-PNTICLLG-------PKDNLGPALTLVFTKSESVNI------IAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEAN-----QIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGV-EAVKAIL 1366
++++P H YIIGP+GSGIK+I+ + D++ Q GS + I ++G K L + + T+ E + +DA H I G G + + + ++ + F +AN QI I G + + + + + + ++ +S+ E+ I+P + I G GG I K+ ++ + I P+ V+++G P V EA+ I
Sbjct: 181 EVQVPSHLHPYIIGPKGSGIKKIMSE----FDVHTQVSQTGSNSDIISIMGLAAKVKQAKVKLEKQINALQTEIEDRALRNFKLMFTLDAK--YHSTITGHKGLFIAQI-CREHDVTIHFPKKANREIQDQITITGYKKNTLAAKDAIMRMLHKIEKTVSK-EIPINPQVRDSIFGFGGKFIHKMNEQFHVDIRLPPKGSWNTNVSVVGLPDNVQEAIDHIF 394
BLAST of Vigilin High density lipoprotein-binding protein vs. UniProt/SwissProt
Match: sp|Q8VDJ3|VIGLN_MOUSE (Vigilin OS=Mus musculus OX=10090 GN=Hdlbp PE=1 SV=1) HSP 1 Score: 368.622 bits (945), Expect = 2.608e-124 Identity = 292/989 (29.52%), Postives = 504/989 (50.96%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQ-KSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPE-PVNSKTISNSHVSAKPGE-----------------------KPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEV------SSTIS-AGVALNKDPISNIKLPQYKLEITGHKDKIT--LAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNK---NSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQY--HRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVL 3391
K +H N + +++ R++IPP SV++ EI +G+++ +AQA + + + Y + K + +E+ K +HKY+IGP+G+ ++EIL++T V V+I ++ T+ L G + LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V E ++ V DL +R+ +E+ ID +H+IG G NI +I+ + S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+D+FPEV INFP P SD++ L GP+ +V++ +QK A LVE+++S V I ++HK IIG+ G I+++ + +NTK +PA + ++ I+ITGK+ + E A R+ +I + +NI E+ + I LH LIG G I S+ E CGG V I FP + S+TV+I+G DV KA+ +L L EE ++ + E ++FL K++K+R++ RI +E +D+ + IIG+++ V + L+ + +V + + + +F + ++ R + + V +S + ++ + +G +++AK I II+D E +T + ++ +++ +++ +I++ Y VQI+ P E PV+S S + GE K + P L I+ + S K + E+N V S TI+ G+A N D G D++ A++E L L V V +++P +IG KG I ++R H+V+I+ P+K N ++ +TG ++ AA+ I++ V ELE+M E VP + H ++IG +G+ I KI + V I P S + N +T+ G +NVE+A + +L
Sbjct: 231 KAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPP---SDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGG-VHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLENVVEDYMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKDCVEAAKKRIQEIIEDLEAQVTVECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSDTIAITGLAANLDRAK-----------AGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSE----------DVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHIL 1191 HSP 2 Score: 102.064 bits (253), Expect = 2.608e-124 Identity = 54/134 (40.30%), Postives = 79/134 (58.96%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHAR 497
IT F +P EER+Y D + K+ EI G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G ++LE T+T++ P+ +DPS IKI G+K+GIE AR
Sbjct: 76 ITQVFHVPLEERKYKDMNQFGEGEQAKICLEIMQRTGAHLELSLAKDQGLSIMVSGKLDAVMKARKDIVARLQTQASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKAR 209 HSP 3 Score: 155.992 bits (393), Expect = 7.507e-37 Identity = 96/311 (30.87%), Postives = 174/311 (55.95%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN---INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQ 3826
I+G K+K AKE + L + +EV VP + + +IG KG I+K+ +V+I +P + + +TG + ++ AK +++ V+EL+ +++R +R+F L++ V +YH K+IG KG I++IR ++ V I P +D ++ITI G++ N E A++ +L V +LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N V V G E+V+ D +++ L ++ D L+ Y EE+ P+K GF +R+APW + ++ S+S++
Sbjct: 953 ISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSG--APDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQ-----VYMKPPAHEESRAPSK-------GFVVRDAPWTSNSSEKAPDMSSSEE 1249 HSP 4 Score: 93.2041 bits (230), Expect = 2.369e-26 Identity = 118/513 (23.00%), Postives = 217/513 (42.30%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESV--NIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQ---IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP------------------------RDLTKK---------VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQV-AAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEEN---DEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDA-DSNTVIIKGVKDDVLKAQVKLHDLVEEITA 2053
+ I G V +A LL + + + I E HK++IG G I+++ D T + + + I ++G +D + A L + E+V + + +D P F+I + G L +++ + + F Q + ++G+ + V + +++ + DL+++++ +E I + ++G G IQ+I ++ N I FP R+ + ++ I G EA K L ++ L +IGQ+G G++++ D F EV I+ P P+ SD I++ G ++D+ A +L + E +F V + KYH IIGR+G I ++ Q P + N D+I ITG +K+ E A + I + ++ + + +H +IG G I + + +V IRFP A D N V + G+ ++V +A + +L EE A
Sbjct: 700 VVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFPA--AEDKDQDLITIIGKEDAVREAQKELEALIQNLENVVEDYMLVD-PKHHRHFVI-RRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-VECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEAL-----EALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEF-EVNIHVPAPELQSDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF--KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLA 1199 HSP 5 Score: 50.0618 bits (118), Expect = 2.369e-26 Identity = 39/139 (28.06%), Postives = 69/139 (49.64%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITR----LVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIFLHF 653
V + G K++V + KY+ + LV+ + IS+ I K+FH+++IG G+ K++ ++T++ P N S I I G + E AR I + + I V I + + N L+ RL ++E + +HF
Sbjct: 554 VQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEE--CGGVHIHF 690 HSP 6 Score: 114.39 bits (285), Expect = 4.611e-24 Identity = 153/691 (22.14%), Postives = 298/691 (43.13%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXE-QGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLI-VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSSTISAGVALN-KDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDL-----EVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
+++ S V IP ++H+ +IG+ G +++L T TK QIP + +++I ITG ++ +E A V I ++ +E+ K H + G + + + G R+ I P P + ++ G K+ + +A ++ + EE ++ ++++ L+++ R SV I ++ S I+ G+ E + L EV KA S S++ ++ + F IG+ N+ K+ K E + + GP +++ A+++I ++ D N + I +D + +++ S ++ I +Y+V ++IP E N I + ++ + N D++ E + G K I EI + I +N DP + Q + G K+++ + + K+ V DL ++VP ++F+ +IG G IKK+R I +P +NS +I+TG + AA+ I+ ++L + + + + +PA+ H LIG KG I I + V I P + S + + I+G +VEKAK ++L + ++ V + H LIG G + +++ I+FP E ++ +++ + G +++V EL
Sbjct: 147 LQTQASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINI--PPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANS--------FTVSSVSAPSWLHR----FIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRME-------------NERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVI-----INFPDPAQKSDIVQLR----GPKNEVEKCTKYMQKM-VADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAE--------VEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFP--AAEDKDQDLITIIGKEDAVREAQKEL 790 HSP 7 Score: 110.538 bits (275), Expect = 7.333e-23 Identity = 141/643 (21.93%), Postives = 286/643 (44.48%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK-SNELF----RIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDER 2521
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++++ +T+ + I R ++ S N I + G K+ + A + L+ + + + ++ H FI G ++G+ N+ SVN+ +IV G E++ + +KK + + + + + +E+ K++IG G ++Q+I + T S+ P D + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP +DV+ ++ L+ ++ I K+H+ +IG+ G I + +IP SE+++ I I G + V+ A + + + + N + I + H +IG+ G RI +++ V I FP P S+ V ++G K++V K + +V ++ ++Y + + ++ + +KK+RE + +I +E + + I G++ N + ARS + I + ++ +++ K N L R+ R M + + + + RGP +++ AK +++H+ ++ + T++ VD R
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQTQASAT-----VPIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPDDPS-NQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR-----------TEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVSAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKF-PEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANC-------EAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQ---TKSFTVDIR 733 HSP 8 Score: 83.1889 bits (204), Expect = 1.474e-14 Identity = 65/251 (25.90%), Postives = 134/251 (53.39%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVL-----DLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRA 3625
+ I G + AK E+++L ++ + + F+ +IG KGE+I+++R +V I P+ K V + G K E+ + + +++M + ++S+++ + Q+H+ +IG G I KIR +++ +I +P+++SN I I G + N E A++ +L+ L + I EV ++I +++H+ LIG++G + + ++ V I FP+ EG ++ V ++G V++ +L+ A
Sbjct: 481 IRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGP-------KNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDL-ANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPV---EGSGSDTVVIRGPSSDVEKAKKQLLHLA 720 HSP 9 Score: 76.2554 bits (186), Expect = 2.084e-12 Identity = 98/450 (21.78%), Postives = 196/450 (43.56%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNE--------------------------GSP---NTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR----DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDA-DSDLISLIGPRKDVDQ-VAAILQKKHAFLVESNFSKKVKI---PFKYHKAIIGREGITIRELTKATNTKFQIP--AASEE 1744
K + + E + + P +++++ G +D V A + + ++A ++ IP++ H+ ++GP+GS I++I +V + ++ N GSP + I + G K+ A + ++ I ++ P LH++IIG+ GS + V++ V P +Q ++ I I G + L +V +LQ+ R +L + +DP IIG G I +IR E +I FP + + +TI G EA + +LK + +E +D+ H + ++IG +GK +++I D F +V I FP A D + +++ G ++V++ + IL + +L + S+ +++ P + ++ +G +R+ +N+ + P ++SEE
Sbjct: 810 KHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-VECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEAL--EALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQ------SDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF-KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQVYMKPPAHEESRAPSKGFVVRDAPWTSNSSEKAPDMSSSEE 1249 HSP 10 Score: 76.2554 bits (186), Expect = 2.174e-12 Identity = 64/287 (22.30%), Postives = 129/287 (44.95%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLFVLDLEVNV------PEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELV 3616
K+ + G KD + AK+ I ++ + DLE V P++F+ +++G KG +I+++ + V IK P++ + +I++G E AAK+ + V ++ + VP HR +IG KG I K+ + V I VP+ + + I I G N+++AK +L V +L++ + +D + H ++IG +GA + Q++ +++V I FP + + + + + G +++ + D ++
Sbjct: 846 KVTLKGAKDCVEAAKKRIQEI-IEDLEAQVTVECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIIISGRKEKCEAAKEALEALVP-------------VTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSDTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHEVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAIL 1118 HSP 11 Score: 61.6178 bits (148), Expect = 5.242e-8 Identity = 34/124 (27.42%), Postives = 65/124 (52.42%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIG 3469
++V+G ++V+ A+K+IV ++ S + +P ++HR +IG GE + + K + +I +P D N+I I G K+ +EKA++EVL + + +E L ++ H + G
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQ-----------TQASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAG 239
BLAST of Vigilin High density lipoprotein-binding protein vs. UniProt/SwissProt
Match: sp|Q9Z1A6|VIGLN_RAT (Vigilin OS=Rattus norvegicus OX=10116 GN=Hdlbp PE=1 SV=1) HSP 1 Score: 365.54 bits (937), Expect = 2.362e-123 Identity = 291/987 (29.48%), Postives = 501/987 (50.76%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQ-KSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPE-PVNSKTISNSHVSAKPGE-----------------------KPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEV------SSTIS-AGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNK---NSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQY--HRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVL 3391
K +H N + +++ R++IPP SV++ EI +G+++ +AQA + + + Y + K + +E+ K +HKY+IGP+G+ ++EIL++T V V+I ++ T+ L G + LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V E ++ V DL +R+ +E+ ID +H+IG G NI +I+ + S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+D+FPEV INFP P S+++ L GP+ +V++ +QK A LVE+++S V I ++HK IIG+ G I+++ + +NTK +PA + ++ IVITGK+ + E A R+ +I + +NI E+ + I LH LIG G I S+ E CGG V I FP + S+TV+I+G DV KA+ +L L EE ++ + E ++FL K++K+R++ RI +E +D+ + IIG+++ V + L+ + +V + + + +F + ++ R + + V +S + ++ + +G +++AK I II+D E +T + ++ +++ +++ +I++ Y VQI+ P E PV+S S + GE K + P L I+ + S K + E+N V S TI+ G+A N D L + K +D+ L L V V +++P +IG KG I ++R H V+I+ P+K N ++ +TG ++ AA+ I++ V ELE+M E VP + H ++IG +G+ I KI + V I P S + N +T+ G +NVE+A + +L
Sbjct: 231 KAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPP---SDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVSAPSWLHRFIIGKKGQNLAKITHQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSEIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIVITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGG-VHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDYMLVDPRHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIVISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSHTIAITGLAANLDRAKAGLLDRVKELQAEQEDR---------ALRSFKLSVTVDPKYHPKIIGGKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSE----------DVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHIL 1191 HSP 2 Score: 102.064 bits (253), Expect = 2.362e-123 Identity = 54/134 (40.30%), Postives = 79/134 (58.96%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHAR 497
IT F +P EER+Y D + K+ EI G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G ++LE T+T++ P+ +DPS IKI G+K+GIE AR
Sbjct: 76 ITQVFHVPLEERKYKDMNQFGEGEQAKICLEIMQRTGAHLELSLAKDQGLSIMVSGKLDAVMKARKDIVARLQTQASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKAR 209 HSP 3 Score: 155.992 bits (393), Expect = 6.719e-37 Identity = 96/311 (30.87%), Postives = 174/311 (55.95%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN---INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQ 3826
I+G K+K AKE + L + +EV VP + + +IG KG I+K+ +V+I +P + + +TG + ++ AK +++ V+EL+ +++R +R+F L++ V +YH K+IG KG I++IR ++ V I P +D ++ITI G++ N E A++ +L V +LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N V V G E+V+ D +++ L ++ D L+ Y EE+ P+K GF +R+APW + ++ S+S++
Sbjct: 953 ISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSHTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGGKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSG--APDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQ-----VYMKPPAHEESKAPSK-------GFVVRDAPWTSNSSEKAPDMSSSEE 1249 HSP 4 Score: 87.4261 bits (215), Expect = 1.045e-24 Identity = 116/513 (22.61%), Postives = 216/513 (42.11%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESV--NIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQ---IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP------------------------RDLTKK---------VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQV-AAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEEN---DEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDA-DSNTVIIKGVKDDVLKAQVKLHDLVEEITA 2053
+ I G V +A LL + + + I E HK++IG G I+++ D T + + + I ++G +D + A L + ++V + + +D P F+I + G L +++ + + F Q + ++G+ + V + +++ + DL+++++ LE I + ++G G IQ+I ++ N I FP R+ + ++ I G EA K L ++ L +IGQ+G G++++ D F EV I+ P P+ S I++ G ++D+ A +L + E +F V + KYH IIG +G I ++ + Q P + N D+I ITG +K+ E A + I + ++ + + +H +IG G I + + +V IRFP A D N V + G+ ++V +A + +L EE A
Sbjct: 700 VVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFPA--AEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDYMLVD-PRHHRHFVI-RRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-LECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIVISGRKEKCEAAKEAL-----EALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEF-EVNIHVPAPELQSHTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGGKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF--KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLA 1199 HSP 5 Score: 50.0618 bits (118), Expect = 1.045e-24 Identity = 39/139 (28.06%), Postives = 69/139 (49.64%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITR----LVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIFLHF 653
V + G K++V + KY+ + LV+ + IS+ I K+FH+++IG G+ K++ ++T++ P N S I I G + E AR I + + I V I + + N L+ RL ++E + +HF
Sbjct: 554 VQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIVITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEE--CGGVHIHF 690 HSP 6 Score: 114.005 bits (284), Expect = 6.312e-24 Identity = 152/691 (22.00%), Postives = 299/691 (43.27%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXE-QGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLI-VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSSTISAGVALN-KDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDL-----EVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
+++ S V IP ++H+ +IG+ G +++L T TK QIP + +++I ITG ++ +E A V I ++ +E+ K H + G + + + G R+ I P P + ++ G K+ + +A ++ + EE ++ ++++ L+++ R SV I ++ S I+ G+ E + L EV KA S S++ ++ + F IG+ N+ K+ K E + + GP +++ A+++I ++ D N + I +D + +++ S ++ I +Y+V ++IP E N I + ++ + N D++ E + G K I EI + I +N DP ++ Q + G K+++ + + K+ V DL ++VP ++F+ +IG G IKK+R I +P +NS +++TG + AA+ I+ ++L + + + + +PA+ H LIG KG I I + V I P + S + + I+G +VEKAK ++L + ++ V + H LIG G + +++ I+FP E ++ +++ + G +++V EL
Sbjct: 147 LQTQASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVKRLEVEKAFHPFIAGPYNRLVGEIMQETGTRINI--PPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANS--------FTVSSVSAPSWLHR----FIIGKKGQNLAKITHQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRME-------------NERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVI-----INFPDPAQKSEIVQLR----GPKNEVEKCTKYMQKM-VADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIVITGKRANCEAARSRILSIQKDLANIAE--------VEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARIIFP--AAEDKDQDLITIIGKEDAVREAQKEL 790 HSP 7 Score: 109.383 bits (272), Expect = 1.339e-22 Identity = 141/643 (21.93%), Postives = 285/643 (44.32%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK-SNELF----RIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDER 2521
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++++ +T+ + I R ++ S N I + G K+ + A + L+ + + + ++ H FI G ++G+ N+ SVN+ +IV G E++ + +KK + + + + + +E+ K++IG G ++Q+I + T S+ P D + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP +DV+ ++ L+ ++ I K+H+ +IG+ G I + +IP SE+++ I I G + V+ A + + + + N + I + H +IG+ G RI +++ V I FP P S V ++G K++V K + +V ++ ++Y + + ++ + +KK+RE + +I +E + + I G++ N + ARS + I + ++ +++ K N L R+ R M + + + + RGP +++ AK +++H+ ++ + T++ VD R
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQTQASAT-----VPIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPDDPS-NQIKITGTKEGIEKARHEVLLISAEQDKRAVKRLEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR-----------TEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVSAPSWLHRF---IIGKKGQNLAKITHQMPKVHIEF---TEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKF-PEVIINFPDPAQKSEIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIVITGKRANC-------EAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQ---TKSFTVDIR 733 HSP 8 Score: 83.5741 bits (205), Expect = 1.245e-14 Identity = 65/251 (25.90%), Postives = 134/251 (53.39%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVL-----DLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRA 3625
+ I G + AK E+++L ++ + + F+ +IG KGE+I+++R +V I P+ K V + G K E+ + + +++M + ++S+++ + Q+H+ +IG G I KIR +++ +I +P+++SN I I G + N E A++ +L+ L + I EV ++I +++H+ LIG++G + + ++ V I FP+ EG ++ V ++G V++ +L+ A
Sbjct: 481 IRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSEIVQLRGP-------KNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIVITGKRANCEAARSRILSIQKDL-ANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPV---EGSGSDTVVIRGPSSDVEKAKKQLLHLA 720 HSP 9 Score: 80.1073 bits (196), Expect = 1.224e-13 Identity = 103/458 (22.49%), Postives = 203/458 (44.32%), Query Frame = 2
Query: 563 RYHQLACHKSNTALHTVLER-KSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNE--------------------------GSP---NTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR----DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDA-DSDLISLIGPRKDVDQ-VAAILQKKHAFLVESNFSKKVKI---PFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQ 1774
R+H+ + L + E + + P +++++ G +D V A + + ++A L+ IP++ H+ ++GP+GS I++I +V + ++ N GSP + I + G K+ A + ++ I ++ P LH++IIG+ GS + V++ V P +Q ++ I I G + L +V +LQ+ R +L + +DP IIG G I +IR E + +I FP + + +TI G EA + +LK + +E +D+ H + ++IG +GK +++I D F +V I FP A D + +++ G ++V++ + IL + +L + S+ +++ P + ++ +G +R+ +N+ + P S ++EI G Q
Sbjct: 810 RHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-LECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIVISGRKEKCEAAKEAL--EALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQ------SHTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGGKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILKIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF-KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQVYMKPPAHEESKAPSKGFVVRDAPWTSNSSEKAPDMS-SSEEIPTFGAQ 1256 HSP 10 Score: 75.8702 bits (185), Expect = 2.779e-12 Identity = 61/286 (21.33%), Postives = 127/286 (44.41%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELV 3616
K+ + G KD + AK+ I ++ + LE +P++F+ +++G KG +I+++ + V IK P++ + ++++G E AAK+ + V ++ + VP HR +IG KG I K+ + V I VP+ + + I I G N+++AK +L V +L++ + +D + H ++IG +GA + Q++ ++ V I FP + + + + + G +++ + D ++
Sbjct: 846 KVTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDYNVQIKFPDREENPVHSVEPSIQENGDEAGEGREAKETDPGSPRRCDIIVISGRKEKCEAAKEALEALVP-------------VTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIHVPAPELQSHTIAITGLAANLDRAKAGLLDRVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGGKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAIL 1118 HSP 11 Score: 60.077 bits (144), Expect = 1.879e-7 Identity = 33/124 (26.61%), Postives = 65/124 (52.42%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIG 3469
++V+G ++V+ A+K+IV ++ S + +P ++HR +IG GE + + K + +I +P D N+I I G K+ +EKA++EVL + + ++ L ++ H + G
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQT-----------QASATVPIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVKRLEVEKAFHPFIAG 239
BLAST of Vigilin High density lipoprotein-binding protein vs. UniProt/SwissProt
Match: sp|Q5R439|VIGLN_PONAB (Vigilin OS=Pongo abelii OX=9601 GN=HDLBP PE=2 SV=1) HSP 1 Score: 328.946 bits (842), Expect = 1.838e-112 Identity = 218/696 (31.32%), Postives = 390/696 (56.03%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQ-KSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPE-PVNS 2626
K +H N + +++ R++IPP SV++ EI +G+++ +AQA + + + Y + K + +E+ K +HKY+IGP+G+ ++EIL++T V V+I ++ T+ L G + LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V E+++ V DL +R+ +E+ ID +H+IG G NI +I+ + S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+D+FPEV INFP P SD++ L GP+ +V++ +QK A LVE+++S V I ++HK IIG+ G I+++ + +NTK +PA + ++ I+ITGK+ + E A R+ +I + +NI E+ + I LH LIG G I S+ E CGG V I FP + S+TV+I+G DV KA+ +L L EE ++ + E ++FL K++K+R++ R+ +E +D+ + IIG+++ V + L+ + +V +++ + +F + ++ R + + V +S + ++ + +G +++AK I II+D E +T + ++ +++ +++ +I++ + VQI+ P E PV+S
Sbjct: 231 KAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPP---SDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVAAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVSVTQEHIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGG-VHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKGCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENPVHS 919 HSP 2 Score: 102.449 bits (254), Expect = 1.838e-112 Identity = 58/146 (39.73%), Postives = 83/146 (56.85%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQ---LIQKNVDE 524
IT F +P EER+Y D + K+ EI G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G ++LE T+T++ P+ +DPS IKI G+K+GIE AR LI DE
Sbjct: 76 ITQVFHVPLEERKYKDMNQFGEGEQAKICLEIMQRTGAHLELSLAKDQGLSIMVSGKLDAVMKARKDIVARLQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDE 221 HSP 3 Score: 142.124 bits (357), Expect = 1.489e-32 Identity = 98/311 (31.51%), Postives = 174/311 (55.95%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSN--INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQ 3826
I+G K+K AKE + L + +EV VP + + +IG KG I+K+ +V+I +P + + +TG + ++ AK ++E V+EL+ +++R +R+F L++ V +YH K+IG KG I++IR ++ V I P + D N ++ITI G++ N E A++ +L V +LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N V V G E+V+ D +++ L ++ D L+ Y EE P++ GF +R+APW A ++ S+S++
Sbjct: 953 ISGRKEKCEAAKEALEALVPVTVEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKGDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSG--APDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQ-----VYMKPPVHEEAKAPSR-------GFVVRDAPWAASSSEKAPDMSSSEE 1249 HSP 4 Score: 110.538 bits (275), Expect = 5.782e-23 Identity = 151/689 (21.92%), Postives = 298/689 (43.25%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALS-INDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLI-VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSSTISAGVALN-KDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDL-----EVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
+++ S V IP ++H+ +IG+ G +++L T TK QIP + +++I ITG ++ +E A V I ++ +E+ K H + G + + + G R+ I P P + ++ G K+ + +A ++ + EE ++ ++++ L+++ R SV I ++ S I+ G+ E + L EV KA S V+ A S ++ +K L +I + M K+++ + + + GP +++ ++ I ++ D N + I +D + +++ S ++ I +Y+V ++IP E N I + ++ + N D++ E + G K I EI + I +N DP + Q + G K+++ + + K+ V DL ++VP ++F+ +IG G IKK+R I +P +NS +I+TG + AA+ I+ ++L + + + + +PA+ H LIG KG I I + V I P + S + + I+G +VEKAK ++L + ++ V + H LIG G + +++ ++FP E ++ +++ + G +++V EL
Sbjct: 147 LQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDERAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINI--PPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFT---VSSVAAPSWLHRFIIGKKGQNLAKITQ-QMPKVHIEFTEGEDK------ITLEGPTEDVSVTQEHIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRME-------------NERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVI-----INFPDPAQKSDIVQLR----GPKNEVEKCTKYMQKM-VADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAE--------VEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFP--AAEDKDQDLITIIGKEDAVREAQKEL 790 HSP 5 Score: 108.997 bits (271), Expect = 2.047e-22 Identity = 141/643 (21.93%), Postives = 285/643 (44.32%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK-SNELF----RIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDER 2521
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++++ +T+ + I R ++ S N I + G K+ + A + L+ + + + ++ H FI G ++G+ N+ SVN+ +IV G E++ + +KK + + + + + +E+ K++IG G ++Q+I + T S+ P D + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP +DV ++ L+ ++ I K+H+ +IG+ G I + +IP SE+++ I I G + V+ A + + + + N + I + H +IG+ G RI +++ V I FP P S+ V ++G K++V K + +V ++ ++Y + + ++ + +KK+RE + +I +E + + I G++ N + ARS + I + ++ +++ K N L R+ R M + + + + RGP +++ AK +++H+ ++ + T++ VD R
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQTQASAT-----VAIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPDDPS-NQIKITGTKEGIEKARHEVLLISAEQDERAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR-----------TEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVAAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTEDVSVTQEHIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKF-PEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANC-------EAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQ---TKSFTVDIR 733 HSP 6 Score: 82.8037 bits (203), Expect = 1.853e-14 Identity = 65/251 (25.90%), Postives = 134/251 (53.39%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVL-----DLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRA 3625
+ I G + AK E+++L ++ + + F+ +IG KGE+I+++R +V I P+ K V + G K E+ + + +++M + ++S+++ + Q+H+ +IG G I KIR +++ +I +P+++SN I I G + N E A++ +L+ L + I EV ++I +++H+ LIG++G + + ++ V I FP+ EG ++ V ++G V++ +L+ A
Sbjct: 481 IRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGP-------KNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDL-ANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPV---EGSGSDTVVIRGPSSDVEKAKKQLLHLA 720 HSP 7 Score: 69.707 bits (169), Expect = 1.894e-10 Identity = 98/452 (21.68%), Postives = 192/452 (42.48%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNE--------------------------GSP---NTICLLGPKDNLGPALTLV--FTKSESVNIIAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR----DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDA-DSDLISLIGPRKDVDQ-VAAILQKKHAFLVESNFSKKVKIPFK---YHKAIIGREGITIRELTKATNTKFQIP--AASEE 1744
K + + E + + P +++++ G + G +AA +Q + L+ IP++ H+ ++GP+GS I++I SV + ++ N GSP + I + G K+ A + + D LH+++IG+ GS + V++ V P +Q ++ I I G + L ++V +LQ+ R +L + +DP IIG G I +IR E + +I FP + + +TI G EA + +L+ + +E +D+ H + ++IG +GK +++I D F +V I FP A D + +++ G ++V++ + IL + +L + S+ +++ K + +A G +R+ A ++ + P ++SEE
Sbjct: 810 KHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAK-GCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENPVHSTEPAVQENGDEAGEGREAKDSDPGSPRRCDIIIISGRKEKCEAAKEALEALVPVTVEVEVPFD----LHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQ------SDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKGDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF-KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQVYMKPPVHEEAKAPSRGFVVRDAPWAASSSEKAPDMSSSEE 1249 HSP 8 Score: 62.003 bits (149), Expect = 4.899e-8 Identity = 34/124 (27.42%), Postives = 65/124 (52.42%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIG 3469
++V+G ++V+ A+K+IV ++ S + +P ++HR +IG GE + + K + +I +P D N+I I G K+ +EKA++EVL + + +E L ++ H + G
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQT-----------QASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDERAVERLEVEKAFHPFIAG 239
BLAST of Vigilin High density lipoprotein-binding protein vs. UniProt/SwissProt
Match: sp|P81021|VIGLN_CHICK (Vigilin OS=Gallus gallus OX=9031 GN=HDLBP PE=2 SV=1) HSP 1 Score: 330.487 bits (846), Expect = 4.400e-112 Identity = 281/988 (28.44%), Postives = 486/988 (49.19%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEEND-EIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQ-KSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFY---PEPVNSKTISNS---------HVSAKP------------GEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSS---------TISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFT---KVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVL 3391
K YH N + +++ R++IPP SV+K EI +G+++ +AQA + + + Y + K + +E+ K +HKY+IG +G+ ++EIL++T V V+I + S T+ L G + LG ALT V+ K+ S + ++ AP WLH+FIIG G NL ++ P I + F + ++I EG E+V E ++ V DL +R E+ +D +H+IG G NI +I+ S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+++FPEV INFP P SD++ L GP+ +V++ +QK A LVE++FS V I ++HK IIG+ G I+++ + +NTK +P + +++ G+++ V+ R+ I ++ +NI E+ + I LH LIG G I S+ E CGG V I FP+ + S TV I+ K + EE +Y + E ++FL ++K+R+ RI SE +D+ + I+G +E V + L+ + +V +++ + +F + ++ R D + V +++ + +G +++AK I II+D E +T + ++ ++I +++ +I++ Y VQI+ P P PV + + A P G + + P L I+ + S K + E+N +V + TI+ G+A N D L + K E+ A++E L L V V +++P +IG KG I ++RT H+V+I+ P+K+ + ++ +TG ++ AA+ I++ V ELE+M E + +L V H ++IG +G+ I KI + V I P S + N +T+ G +NVE+A + +L
Sbjct: 230 KVYHPFIAGPYNKLVSELMQDTGTRINIPPPSVNKTEIVFTGEKEQLAQAVARVKKIYEEKKKKTTTIAVEVKKSQHKYVIGRKGNSLQEILEKTGVSVEIPP---TDSSSETVILRGEPEKLGQALTEVYAKANSFTVSSVSAPSWLHRFIIGLFGQNLAKITQQMPKIHIEFTEGEDKITSEGPTEDVNVAQEQIEVMVKDLINRTDYAEINVDHKFHRHLIGKNGANINRIKDLYKVSVRIPPDNEKSNLIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIINFPDPAHKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSFSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPGREQATQRQLLSQGREQTVKLLRHRILAIQKELANITEVEVSIPSKLHNSLIGAKGRFIRSIMEECGG-VHIHFPTEGSGSATVTIRAQPRTWRKPRSSCCTWAEEKQTKSYTVDLRAKPEYHKFLIGKGGGNIRKVRDNTGARIIFPTSEDKDQELITIMGTEEAVKEAQKELEALIKNLDNVVEDSMVVDPKHHRHFVIRRGQVLREIADEYGGVMVRLPTVSGTQSDK--VTLKGAKDCVEAAKKRIQEIIEDLEAQVTIECTIPQKFHRSIMGPKGSRIQQITRDYGVQIKFPDREENPAPVAEPALQENGEEGGEGKDGKDADPSSPRKCDIIIISGRREKCEAAKEALQALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPAPELQSSDIITIT-GLAANLDRAKAGLLERVK-ELQ--------AEQEDRALRSFKLTVTVDPKYHPKIIGRKGAVITQIRTEHEVNIQFPDKDDESQAQDQITITGYEKNAEAARDAIMKIVGELEQMVSE----DVTLDHRV----HARIIGARGKAIRKIMDEFKVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHIL 1193 HSP 2 Score: 99.3673 bits (246), Expect = 4.400e-112 Identity = 53/137 (38.69%), Postives = 80/137 (58.39%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
IT F +P EER+Y D + K+ +I G +E ++ DQ L + +SG+ + V +A+K I +QTQ ++ IPKE HR +IG G ++LE T+T++ P+ +DPS IKI G+K+GIE AR I
Sbjct: 75 ITQVFHVPLEERKYKDMNQFGEGEQAKICLDIMQKTGAHLELSLAKDQGLSIMVSGKLEAVMKARKEIVARLQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEI 211 HSP 3 Score: 159.844 bits (403), Expect = 4.500e-38 Identity = 100/313 (31.95%), Postives = 178/313 (56.87%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVI-VTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNI---NEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEI--NDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASD 3823
I+G ++K AKE + L + +EV VP + + +IG KG I+K+ +V+I++P + + +I +TG + ++ AK ++E V+EL+ +++R +R+F L + V +YH K+IG KG I++IRT++ V I P +D ++ITI G++ N E A++ ++ V +LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N V V G E+V+ D +++ L ++ N+ MQ+ SS+ ES P+K GF +R+AP V N+ S+S+
Sbjct: 954 ISGRREKCEAAKEALQALVPVTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPAPELQSSDIITITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLTVTVDPKYHPKIIGRKGAVITQIRTEHEVNIQFPDKDDESQAQDQITITGYEKNAEAARDAIMKIVGELEQMVSEDVTLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSG--APDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDNEAMQVYMKPSSHEESKV-------PSK-------GFVVRDAPCGTVNNEKAPDMSSSE 1250 HSP 4 Score: 122.094 bits (305), Expect = 2.032e-26 Identity = 171/754 (22.68%), Postives = 333/754 (44.16%), Query Frame = 2
Query: 1439 LIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLV-----ESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXX-XXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHD-----LVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRIIGQKENV---LNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRD--------NMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIPFYPEPVNSKTISNSHVSAKPGEK--PYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN--GSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVD 3595
+IG+ G+ L++++ + +I P PD S+ I + G ++ +++ +H L+ + +++ + YH I G + EL + T T+ IP S EIV TG+++ + A+ RV IYE++ +E+ K+ H +IG+ GN + + E G V++ P D+ S TVI++G + + +A +++ V ++A ++ F + L F K+ + + +E EDK + G E+V +EV+ K ++ T IN F + IG++ ++ K++V + ++ G Q ++ AK E++ + EN T++LI++++ + I ++ EI +K+ +V I +P+P + I EK YM + L N + I + + N G NI +I E ++ I D + Q +L G + + L + I+ + + ++EV++P + + +LIGAKG I+ + V I P + S V + + + + EE ++ +++++ L +YH+ LIG G I K+R RI P S+D + ITI G ++ V++A+ E+ A + L++ + + + +D + H + RG L ++ +Y V + P G +++ V +KG K+ V+
Sbjct: 164 VIGKNGEKLQDLELKT-ATKIQIPRPDDPSNQIKITGTKEGIEKA------RHEILLISAEQDKRAVERLDVEKVYHPFIAGPYNKLVSELMQDTGTRINIPPPSVNKTEIVFTGEKEQLAQAVARVKKIYEEKKKKTTTIAVEVKKSQHKYVIGRKGNSLQEILEKTG--VSVEIPPTDSSSETVILRGEPEKLGQALTEVYAKANSFTVSSVSAPSWLHRFIIGL------FGQNLAKITQQMPKIHIEFTEGEDK--ITSEGPTEDVNVAQEQIEVMVKD------LINRTDYAEINVDHKFHR----HLIGKNGANINRIKDLYKVSVRIPPDNE---KSNLIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKFPEVIIN---FPDPAHKSDIVQLRGPKNEVEKCTKYMQKM---------VADLVENSFSISVPIFKQFHKNIIGKGGANIKKIREESNTKI--------DLPGREQATQRQLLSQGREQTVKLLRHRILAIQKELANITEVEVSIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPTEGSGSATVTIRAQPRTWRKPRSSCCTWAEE-------KQTKSYTVDLRAKPEYHKFLIGKGGGNIRKVRDNTGARIIFPTSEDKDQELITIMGTEEAVKEAQKELEALIKNLDNVVEDSMVVDPKHHRHFVIRRGQVLREIADEYGGVMVRLPT--VSGTQSDKVTLKGAKDCVE 858 HSP 5 Score: 103.219 bits (256), Expect = 1.232e-20 Identity = 114/505 (22.57%), Postives = 231/505 (45.74%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG---KSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI 2161
I +SG + V +A I+ + Q AT + IPKE H+++IG G ++++ +T+ + I R ++ S N I + G K+ + A + L+ + + + +D H FI G K S L+ + + NI ++ +IV G E++ + +KK + + + + + +E+ K++IG G ++Q+I ++T S+ P D + + V + G P + + + + + H +IG G+ L +I + P++ I F D I+ GP +DV+ ++ L+ ++ + K+H+ +IG+ G I + +IP +E+++ I I G + V+ A K + + + N + I + H +IG+ G RI ++E V I FP P S+ V ++G K++V K + +V ++ +++ + + ++ + +KK+RE + +I
Sbjct: 126 IMVSGKLEAVMKARKEIVARLQTQASAT-----VAIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPDDPS-NQIKITGTKEGIEKARHEILLISAEQDKRAVERLDVEKVYHPFIAGPYNKLVSELMQDTGTRINIPPPSVNK-TEIVFTGEKEQLAQAVARVKKIYEEKKKKTTTIAVEVKKSQHKYVIGRKGNSLQEILEKTGVSVEIPPTDSSSETVILRGEPEKLGQALTEVYAKANSFTVSSVSAPSWLHRF---IIGLFGQNLAKITQQMPKIHIEF---TEGEDKITSEGPTEDVNVAQEQIEVMVKDLINRTDYAEINVDHKFHRHLIGKNGANINRIKDLYKVSVRIPPDNEKSNLIRIEGDPQGVQQAKKELLELASRMENERTKDLIIEQKFHRTIIGQKGERIREIREKF-PEVIINFPDPAHKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSFSISVPIFKQFHKNIIGKGGANIKKIREESNTKI 614 HSP 6 Score: 94.3597 bits (233), Expect = 6.096e-18 Identity = 146/694 (21.04%), Postives = 291/694 (41.93%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRET--YSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIG--RDNMCKLNVXXXXXXXXXXE-QGILEFRGPVQNIKSAKDEIIHIIDDYEN-TITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYK---LEITGHKDKITLAKEEIIKLFVLDL-----EVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVT-GSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
+++ S V IP ++H+ +IG+ G +++L T TK QIP + +++I ITG ++ +E A + I ++ +++ K H + G ++ L + G R+ I P P + ++ G K+ + +A ++ + EE ++ ++++ L+++ E SV I + S I+ G+ E + L EV KA S S++ ++ + F IG N+ K+ K E + + GP +++ A+++I ++ D N T + VD + +++ + ++ I Y+V ++IP E N I + +K + N D++ E + G K I EI + I N P +K +++ G K+++ + + K+ V DL ++VP ++F+ +IG G IKK+R I +P + + +++ G ++V + I+ +EL + + + + +P++ H LIG KG I I + V I P++ S +TI+ K ++ + + ++ V L H LIG G N+ +++ I+FP +E ++ ++ + GT+E+V EL
Sbjct: 146 LQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEILLISAEQDKRAVERLDVEKVYHPFIAGPYNKLVSELMQDTGTRINI--PPPSVNKTEIVFTGEKEQLAQAVARVKKIYEEKKKKTTTIAVEVKKSQHKYVIGRKGNSLQEILEKTGVSVEIPPTDSSSETVILRGEPEKLGQALTEVYAKANS--------FTVSSVSAPSWLHR----FIIGLFGQNLAKITQQMPKIHIEFTEGEDKITSEGPTEDVNVAQEQIEVMVKDLINRTDYAEINVDHKFHRHLIGKNGANINRIKDLYKVSVRIPPDNEKSNLIRIEGDPQGVQQAKKELLELASRME-------------NERTKDLIIEQKFHRTIIGQKGERIREIREKFPEV-----------IINFPDPAHKSDIVQLRGPKNEVEKCTKYMQKM-VADLVENSFSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPGREQATQRQLLSQGREQTVKLLRHRILAIQKELANITE--------VEVSIPSKLHNSLIGAKGRFIRSIMEECGGVHIHFPTEGSGSATVTIRAQPRTWRKPRSSCCTWAEEKQTKSYTVDLRAKPEYHKFLIGKGGGNIRKVRDNTGARIIFPT--SEDKDQELITIMGTEEAVKEAQKEL 790 HSP 7 Score: 81.6481 bits (200), Expect = 3.873e-14 Identity = 67/289 (23.18%), Postives = 132/289 (45.67%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPS---QDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELV 3616
K+ + G KD + AK+ I ++ + +E +P++F+ +++G KG +I+++ + V IK P++ K +I++G E AAK+ + V ++ + VP HR +IG KG I K+ + V I VP+ Q S+I ITI G N+++AK +L V +L++ + +D + H ++IG +GA + Q++ +++V I FP + E + + + + G +++ + D ++
Sbjct: 847 KVTLKGAKDCVEAAKKRIQEIIEDLEAQVTIECTIPQKFHRSIMGPKGSRIQQITRDYGVQIKFPDREENPAPVAEPALQENGEEGGEGKDGKDADPSSPRKCDIIIISGRREKCEAAKEALQALVP-------------VTIEVEVPFDLHRYIIGQKGSGIRKMMDEFEVNIQVPAPELQSSDI--ITITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLTVTVDPKYHPKIIGRKGAVITQIRTEHEVNIQFPDKDDESQAQDQITITGYEKNAEAARDAIM 1120 HSP 8 Score: 64.3142 bits (155), Expect = 7.694e-9 Identity = 38/144 (26.39%), Postives = 72/144 (50.00%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFP 3529
++V+G E+V+ A+KEIV ++ S + +P ++HR +IG GE + + K + +I +P D N+I I G K+ +EKA++E+L + + +E L+++ H + G + +L + I P
Sbjct: 126 IMVSGKLEAVMKARKEIVARLQT-----------QASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEILLISAEQDKRAVERLDVEKVYHPFIAGPYNKLVSELMQDTGTRINIP 258
BLAST of Vigilin High density lipoprotein-binding protein vs. UniProt/SwissProt
Match: sp|Q00341|VIGLN_HUMAN (Vigilin OS=Homo sapiens OX=9606 GN=HDLBP PE=1 SV=2) HSP 1 Score: 326.635 bits (836), Expect = 1.234e-111 Identity = 214/687 (31.15%), Postives = 384/687 (55.90%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQ-KSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
K +H N + +++ R++IPP SV++ EI +G+++ +AQA + + + Y + K + +E+ K +HKY+IGP+G+ ++EIL++T V V+I ++ T+ L G + LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG E+V E ++ V DL +R+ +E+ ID +H+IG G NI +I+ + S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ ++EI+D+FPEV INFP P SD++ L GP+ +V++ +QK A LVE+++S V I ++HK IIG+ G I+++ + +NTK +PA + ++ I+ITGK+ + E A R+ +I + +NI E+ + I LH LIG G I S+ E CGG V I FP + S+TV+I+G DV KA+ +L L EE ++ + E ++FL K++K+R++ R+ +E +D+ + IIG+++ V + L+ + +V +++ + +F + ++ R + + V +S + ++ + +G +++AK I II+D E +T + ++ +++ +++ +I++ + VQI+ P
Sbjct: 231 KAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPP---SDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVAAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGG-VHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDK---VTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFP 910 HSP 2 Score: 101.679 bits (252), Expect = 1.234e-111 Identity = 54/134 (40.30%), Postives = 79/134 (58.96%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHAR 497
IT F +P EER+Y D + K+ EI G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G ++LE T+T++ P+ +DPS IKI G+K+GIE AR
Sbjct: 76 ITQVFHVPLEERKYKDMNQFGEGEQAKICLEIMQRTGAHLELSLAKDQGLSIMVSGKLDAVMKARKDIVARLQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKAR 209 HSP 3 Score: 157.147 bits (396), Expect = 3.543e-37 Identity = 97/311 (31.19%), Postives = 173/311 (55.63%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN---INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQ 3826
I+G K+K AKE + L + +EV VP + + +IG KG I+K+ +V+I +P + + +TG + ++ AK ++E V+EL+ +++R +R+F L++ V +YH K+IG KG I++IR ++ V I P +D ++ITI G++ N E A++ +L V +LE + E + +D R+H+R+IG+RG + ++ ++KV+I FP G + N V V G E+V+ D +++ L ++ D L+ Y EE P++ GF +R+APW A ++ S+S++
Sbjct: 953 ISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSG--APDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQ-----VYMKPPAHEEAKAPSR-------GFVVRDAPWTASSSEKAPDMSSSEE 1249 HSP 4 Score: 94.3597 bits (233), Expect = 1.257e-26 Identity = 116/512 (22.66%), Postives = 218/512 (42.58%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDA----PGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQ---IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP---------------------------RDLTK------KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQV-AAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEEN---DEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDA-DSNTVIIKGVKDDVLKAQVKLHDLVEEITA 2053
+ I G V +A LL + + + I E HK++IG G I+++ D T V + + I ++G +D + A + ++++ + D+ P F+I + G L +++ + + F Q + ++G+ + V + +++ + DL+++++ LE I + ++G G IQ+I ++ + I FP +D ++ I G EA K L ++ L +IGQ+G G++++ D F EV I+ P P+ SD+I++ G ++D+ A +L++ E +F V + KYH IIGR+G I ++ + Q P + N D+I ITG +K+ E A + I + ++ + + +H +IG G I + + +V IRFP A D N V + G+ ++V +A + +L EE A
Sbjct: 700 VVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPA--AEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVI-RRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-LECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEAL-----EALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEF-EVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF--KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLA 1199 HSP 5 Score: 49.6766 bits (117), Expect = 1.257e-26 Identity = 39/139 (28.06%), Postives = 69/139 (49.64%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITR----LVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIFLHF 653
V + G K++V + KY+ + LV+ + IS+ I K+FH+++IG G+ K++ ++T++ P N S I I G + E AR I + + I V I + + N L+ RL ++E + +HF
Sbjct: 554 VQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEE--CGGVHIHF 690 HSP 6 Score: 113.235 bits (282), Expect = 9.814e-24 Identity = 152/689 (22.06%), Postives = 300/689 (43.54%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALS-INDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLI-VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSSTISAGVALN-KDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDL-----EVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
+++ S V IP ++H+ +IG+ G +++L T TK QIP + +++I ITG ++ +E A V I ++ +E+ K H + G + + + G R+ I P P + ++ G K+ + +A ++ + EE ++ ++++ L+++ R SV I ++ S I+ G+ E + L EV KA S V+ A S ++ +K L +I + M K+++ + + + GP +++ A+++I ++ D N + I +D + +++ S ++ I +Y+V ++IP E N I + ++ + N D++ E + G K I EI + I +N DP + Q + G K+++ + + K+ V DL ++VP ++F+ +IG G IKK+R I +P +NS +I+TG + AA+ I+ ++L + + + + +PA+ H LIG KG I I + V I P + S + + I+G +VEKAK ++L + ++ V + H LIG G + +++ ++FP E ++ +++ + G +++V EL
Sbjct: 147 LQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINI--PPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFT---VSSVAAPSWLHRFIIGKKGQNLAKITQ-QMPKVHIEFTEGEDK------ITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRME-------------NERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVI-----INFPDPAQKSDIVQLR----GPKNEVEKCTKYMQKM-VADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAE--------VEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFP--AAEDKDQDLITIIGKEDAVREAQKEL 790 HSP 7 Score: 109.768 bits (273), Expect = 1.230e-22 Identity = 141/643 (21.93%), Postives = 286/643 (44.48%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK-SNELF----RIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDER 2521
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++++ +T+ + I R ++ S N I + G K+ + A + L+ + + + ++ H FI G ++G+ N+ SVN+ +IV G E++ + +KK + + + + + +E+ K++IG G ++Q+I + T S+ P D + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP +DV+ ++ L+ ++ I K+H+ +IG+ G I + +IP SE+++ I I G + V+ A + + + + N + I + H +IG+ G RI +++ V I FP P S+ V ++G K++V K + +V ++ ++Y + + ++ + +KK+RE + +I +E + + I G++ N + ARS + I + ++ +++ K N L R+ R M + + + + RGP +++ AK +++H+ ++ + T++ VD R
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQTQASAT-----VAIPKEHHRFVIGKNGEKLQDLELKTATKIQI--PRPDDPS-NQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNR-----------TEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVAAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKF-PEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANC-------EAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQ---TKSFTVDIR 733 HSP 8 Score: 77.0258 bits (188), Expect = 5.846e-20 Identity = 98/450 (21.78%), Postives = 196/450 (43.56%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNE--------------------------GSP---NTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR----DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDA-DSDLISLIGPRKDVDQ-VAAILQKKHAFLVESNFSKKVKI---PFKYHKAIIGREGITIRELTKATNTKFQIP--AASEE 1744
K + + E + + P +++++ G +D V A + + ++A L+ IP++ H+ ++GP+GS I++I SV + ++ N GSP + I + G K+ A + ++ I ++ P LH+++IG+ GS + V++ V P +Q ++ I I G + L ++V +LQ+ R +L + +DP IIG G I +IR E + +I FP + + +TI G EA + +L+ + +E +D+ H + ++IG +GK +++I D F +V I FP A D + +++ G ++V++ + IL + +L + S+ +++ P + +A G +R+ ++ + P ++SEE
Sbjct: 810 KHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVT-LECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEAL--EALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQ------SDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEF-KVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQVYMKPPAHEEAKAPSRGFVVRDAPWTASSSEKAPDMSSSEE 1249 HSP 9 Score: 44.669 bits (104), Expect = 5.846e-20 Identity = 33/118 (27.97%), Postives = 56/118 (47.46%), Query Frame = 3
Query: 174 KLLTEICANCG-VRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQ----FEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPST-LIKIKGSKDGIEHARQLIQ 509
+L+ I CG V + V S V I G V +AKK + L + + F + + E+H+ LIG G +++ ++T +V FP D LI I G +D + A++ ++
Sbjct: 674 RLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELE 791 HSP 10 Score: 82.8037 bits (203), Expect = 1.807e-14 Identity = 59/218 (27.06%), Postives = 121/218 (55.50%), Query Frame = 2
Query: 2981 VPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRA 3625
+ + F+ +IG KGE+I+++R +V I P+ K V + G K E+ + + +++M + ++S+++ + Q+H+ +IG G I KIR +++ +I +P+++SN I I G + N E A++ +L+ L + I EV ++I +++H+ LIG++G + + ++ V I FP+ EG ++ V ++G V++ +L+ A
Sbjct: 514 IEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGP-------KNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDL-ANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPV---EGSGSDTVVIRGPSSDVEKAKKQLLHLA 720 HSP 11 Score: 73.9442 bits (180), Expect = 8.784e-12 Identity = 62/286 (21.68%), Postives = 126/286 (44.06%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--------------------------------KFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELV 3616
K+ + G KD + AK+ I ++ + LE +P++F+ +++G KG +I+++ V IK P++ + +I++G E AAK+ + V ++ + VP HR +IG KG I K+ + V I VP+ + + I I G N+++AK +L V +L++ + +D + H ++IG +GA + Q++ ++ V I FP + + + + + G +++ + D ++
Sbjct: 846 KVTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEALEALVP-------------VTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAIL 1118 HSP 12 Score: 62.003 bits (149), Expect = 4.657e-8 Identity = 34/124 (27.42%), Postives = 65/124 (52.42%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIG 3469
++V+G ++V+ A+K+IV ++ S + +P ++HR +IG GE + + K + +I +P D N+I I G K+ +EKA++EVL + + +E L ++ H + G
Sbjct: 127 IMVSGKLDAVMKARKDIVARLQT-----------QASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAG 239
BLAST of Vigilin High density lipoprotein-binding protein vs. TrEMBL
Match: A0A1S3I7W9 (vigilin OS=Lingula unguis OX=7574 GN=LOC106161524 PE=4 SV=1) HSP 1 Score: 501.516 bits (1290), Expect = 1.607e-161 Identity = 335/1082 (30.96%), Postives = 584/1082 (53.97%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDL-TKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNG-VDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS-NINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWD 3781
K YH C +N + + ++ R+ +PP SV K+EI +SG+++GV +A + +++ Y + K Q + +E+ K +HKY+IGPR + EIL T V V++ L+ S TI L G +D LGPALT+V++K+ SV I + AP WLH+FIIG+ G+ + ++ + P + + F + ++IVIEG P+EV + + L + DLQ+R++ E++++ KHIIG G NI +I+ +T +I P D +++ I G P GVE KA L++ +E +EN + KDI+ +IG +G +KE++D+F +V+I FP P S++++L GP+ DV++ LQ+ H L+ N+ +V I ++HK IIG+ G TI+++ + T+T+ +P+ + ++D IVITGK+++VE A ++ I ++ +NI EI IEI H +IG G I S+ E CGG V +RFP S+ V+++G KDDV KA+ +L L +E + + + ++FL ++K+RE+ R+ S+ D+ + IIG +E+V L+ E+ +V +++ +F + E+ + ++V +S ++ + +G I+ AK I I+ + E+ +T ++ ++ + + +++ E+++ + V I+ P P+ +N K KP +NG VD + H+ +EH+ + K +I + ITG K+ AKE ++ L + EVNVP +F+ +IG +G ++K+ V+I IP +++ V VTG +V A + + VEELE+ K++R ++NFSL + V YH K+IG +G ISKIR V I P + S N + ITI G++ + ++AK+E++ V +L++ I E +N+D R+HSR+IG +G + ++ YKV+I FP R ++ + ++V + G +++V D L + L ++ DR + + + + P++ H GFQ+R+APWD
Sbjct: 226 KIYHPFICGPNNALMKELQDKTGARIHVPPPSVMKDEIVVSGEKEGVHEAKATIMKIYEEKKRKCQTVSVEVRKSQHKYVIGPRAGALNEILQATGVSVEVPP--LDSPS-ETITLRGEQDKLGPALTMVYSKANSVVIAEVVAPAWLHRFIIGRQGATIRKITQDLPKVHIEFTEGQDKIVIEGPPDEVEQAQKALGEITQDLQTRMAYAELDVNQKYHKHIIGKNGANITRIKNDTGVAIRIPSDSEGSRIIRIEGDPKGVEKAKAELMEMVEKMENERSKDIMIEQRFHRTIIGAKGDKIKEVRDKFNQVQITFPDPGKKSNIVTLRGPKNDVEKCYKYLQQMHQELIAVNYQAEVHIFKQFHKNIIGKGGATIKKIREETDTRIDLPSEASDSDVIVITGKRQNVETAKAKIEAIQKELANIKEITIEIPHKHHNSIIGAKGRLIRSIMEDCGG-VIVRFPPEGGKSDKVVLRGPKDDVEKAKKELLALADERQTHGFTAEIKAKPDFHKFLIGRNGANIRKVRESTGARVIFPTSKDADQETISIIGTEESVSTAKTELEAMIKELENVVEGEISVDPKYHRHFVARRGEVLHTIAADYGGVSVSFPRSGMKSDK---VVLKGAKDCIEGAKKRIAEIVAELESQVTIECVIPQQYHRTVMGTKGSRVQEVTKDHNVGIKFPDRPQ-------ANGAGDGKGNAKPQVNGDVDVN------------GDTHVDGAGDAEHDQDKPKKQDI-------------------------------ILITGKKEDCEAAKEALMALVPITEEVNVPFDFHRFIIGQRGRDVRKMMEEFDVNISIPPADNRSDIVKVTGPRANVARAVEGLNARVEELEKEKEDRALKNFSLEVRVDPAYHPKIIGRRGAVISKIRDAFDVNIQFPDKGSENQDVITITGYEKSAKEAKDEIMKLVGELDNMITEEVNLDHRVHSRIIGQKGRQVRKIMDDYKVDIKFP-RSSDNDNPDLVVITGMEDNVLDAKDYLQNLEEEYLQDVLDRESMRQYMHPSKQEQSHPKQ---------NHGPGFQVRDAPWD 1240 HSP 2 Score: 100.908 bits (250), Expect = 1.607e-161 Identity = 52/150 (34.67%), Postives = 91/150 (60.67%), Query Frame = 3
Query: 108 TDTFVIPYEERRYID------SESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIG 539
T F +P EERR+ + E+ + K+ +I GV +E ++ DQSL V I+G+ + V +A++ + +QTQ +++ IP+E HR ++G G +ELE NT+T+++ P+ + S +I+I G+K+GI+ AR +Q DE +K+
Sbjct: 69 TQVFRVPLEERRFQEINEHGFGEAGEQAKICKDIMQKTGVAIEMSLAKDQSLTVVITGKIENVMKARRMVVNQLQTQANVTMKIPREHHRFILGKSGKKLQELELNTTTKITIPRPEENSDVIRIVGTKEGIDKARHELQLITDEQAKLA 218 HSP 3 Score: 140.969 bits (354), Expect = 8.788e-30 Identity = 183/836 (21.89%), Postives = 359/836 (42.94%), Query Frame = 2
Query: 1439 LIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQ------KKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXX-XXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHD-----LVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNE---------LFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN--GSKYHNISEINSEVSSTIS-AGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGD 3850
++G+ GK L+E+ + +I P P+ +SD+I ++G ++ +D+ LQ K AF +++ I YH I G ++EL T + +P S DEIV++G+++ V +A + IYE++ + +E+ K+ H +IG +N + ++ G V++ P D+ S T+ ++G +D + A ++ ++ E+ A + F + + ++ DL + +E +DK + E + +E QKA E+ + +A + +L QK ++ + RI D + +P+ S I+ G + ++ AK E++ +++ EN ++++++++R + I +++ E+ K+ Q+QI F P+P I EK Y + I + + N G I +I E + I A + D I ITG + + AK +I + + ++ + +P + + ++IGAKG I+ + V ++ P + K KV++ G + V AKKE++ +E R+ F+ + +H+ LIG G I K+R R+ P S+D++ I+I G +++V AK E+ A + +LE+ + +++D + H + RG L + Y V + FP G + ++ V +KG K+ C + R ++ E+ ++ +E Y + + H G + + P DG ++ V+ GD
Sbjct: 160 ILGKSGKKLQEL-ELNTTTKITIPRPEENSDVIRIVGTKEGIDKARHELQLITDEQAKLAF-------ERLPIAKIYHPFICGPNNALMKELQDKTGARIHVPPPSVMKDEIVVSGEKEGVHEAKATIMKIYEEKKRKCQTVSVEVRKSQHKYVIGPRAGALNEILQATG--VSVEVPPLDSPSETITLRGEQDKLGPALTMVYSKANSVVIAEVVAPAWLHRFIIGRQGATIRKITQDLP------KVHIEFTEGQDKIVI------EGPPDEVEQAQKALGEITQDLQTRMAYA--ELDVNQKYHKHIIGKNGANITRIKNDTGVAIRIPSDSEGSR-----IIRIEGDPKGVEKAKAELMEMVEKMENERSKDIMIEQRFHRTIIGAKGDKIKEVRDKFN-QVQITF-PDPGKKSNIVTLRGPKNDVEKCYKYLQQMHQELIAVNYQAEVH-------IFKQFHKNIIGKGGATIKKIREETDTRIDLPSEASDSDVIV----------ITGKRQNVETAKAKIEAIQKELANIKEITIEIPHKHHNSIIGAKGRLIRSIMEDCGGVIVRFPPEGGKSDKVVLRGPKDDVEKAKKELLALADE-------RQTHGFTAEIKAKPDFHKFLIGRNGANIRKVRESTGARVIFPTSKDADQETISIIGTEESVSTAKTELEAMIKELENVVEGEISVDPKYHRHFVARRGEVLHTIAADYGGVSVSFPRSGMKSDK---VVLKGAKD----CIEGAKKRIAEIVAELESQVTIECVIPQQYHRTVMGTKGSRVQEVTKDHNVGIKFPDRPQANGAGDGKGNAKPQVNGDVDVNGD 933 HSP 4 Score: 127.487 bits (319), Expect = 1.174e-25 Identity = 149/619 (24.07%), Postives = 279/619 (45.07%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEA---NQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNT---LEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYEN 2488
+ I+G + V +A +++ NQ++ T + ++IP+E H++I+G G ++E+ T+ + I R E S + I ++G K+ + A L L+ + + + H FI G + + + +L +K ++ + ++IV+ G E V E + K + + + + +E+ K++IG + +I + T S+ P D + +T+ G + ++ ++ + + H +IG+QG +++I P+V I F D I + GP +V+Q L + L ++ + KYHK IIG+ G I + T +IP+ SE + I I G K VE A + + EK N I I + H +IG G++I +++ +V I FP P SN V ++G K+DV K L + +E+ A NY+ + + ++ + +KK+RE RI SEA D + I G+++NV +E +QK + + I E N + K + I D C + + +++ +L RGP +++ AK E++ + D+ +
Sbjct: 122 VVITGKIENVMKARRMVV---NQLQ-TQANVTMKIPREHHRFILGKSGKKLQELELNTTTKITI--PRPEENS-DVIRIVGTKEGIDKARHELQLITDEQAKLAFERLPIAKIYHPFICGPNNALMKELQ-DKTGARIHVPPPSVMKDEIVVSGEKEGVHEAKATIMKIYEEKKRKCQTVSVEVRKSQHKYVIGPRAGALNEILQATGVSVEVPPLDSPSETITLRGEQDKLGPALTMVYSKANSVVIAEVVAPAWLHRF---IIGRQGATIRKITQDLPKVHIEF---TEGQDKIVIEGPPDEVEQAQKALGEITQDLQTRMAYAELDVNQKYHKHIIGKNGANITRIKNDTGVAIRIPSDSEGSRIIRIEGDPKGVEKAKAELMEMVEKMENERSKDIMIEQRFHRTIIGAKGDKIKEVRDKF-NQVQITFPDPGKKSNIVTLRGPKNDVEKCYKYLQQMHQELIAVNYQAEVHIFKQFHKNIIGKGGATIKKIREETDTRIDLPSEASDSDVIVITGKRQNVETAKAKIEAIQKELANIKEITIEIPHKHHNSI-IGAKGRLIRSIMED--CGGVIVRFPPEGGKSDKVVL--RGPKDDVEKAKKELLALADERQT 720 HSP 5 Score: 93.9745 bits (232), Expect = 2.259e-15 Identity = 125/492 (25.41%), Postives = 236/492 (47.97%), Query Frame = 2
Query: 824 KEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQL-RFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLX-XXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXX-XXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASN-----YEQHFQMTLEDYEFLFMSADLKLKKLRETYS-VRISEAEDKSHLRII---GQKENVLNTLEVLQKARSEM 2257
K+I+ +T V ++++ L + T+ + G +N+ A +V + ++ + + P H+FI+GKSG L +L +N I + R E+ ++ I I G+ +E I+ + + + + D Q++L+ + I + I G +++++ +T I+ P + K + + G GV KA ++K E + + + +IG + L EI + V + PP D+ S+ I+L G + + ++ K +V + +V P H+ IIGR+G TIR++T+ K I +E D+IVI G +VE A K + I + ++ + +++++ H +IGK G I +K G V IR PS S + I+G V KA+ +L ++VE++ EQ F T+ + D K+K++R+ ++ V+I+ + I+ G K +V + LQ+ E+
Sbjct: 100 KDIMQKTGVAIEMS---LAKDQSLTVVITGKIENVMKARRMVVNQLQTQANVTMKIPREHHRFILGKSGKKLQELELNTTTKITIPRPEENSDVIRIVGT-KEGIDKARHELQLITDEQAKLAFERLPIAKIYHPFICGPNNALMKELQDKTGARIHVPPPSVMKDEIVVSGEKEGVHEAKATIMKIYEEKKRKCQTVSVEVRKSQHKYVIGPRAGALNEIL-QATGVSVEVPPLDSPSETITLRGEQDKLGPALTMVYSKANSVVIA----EVVAPAWLHRFIIGRQGATIRKITQDL-PKVHI-EFTEGQDKIVIEGPPDEVEQAQKALGEITQDLQTRMAYAELDVNQKYHKHIIGKNGANITRIKNDTG--VAIRIPSDSEGSRIIRIEGDPKGVEKAKAELMEMVEKMENERSKDIMIEQRFHRTI-----IGAKGD-KIKEVRDKFNQVQITFPDPGKKSNIVTLRGPKNDVEKCYKYLQQMHQEL 572
BLAST of Vigilin High density lipoprotein-binding protein vs. TrEMBL
Match: A0A3Q0JY67 (vigilin isoform X3 OS=Ciona intestinalis OX=7719 GN=LOC100176627 PE=4 SV=1) HSP 1 Score: 506.138 bits (1302), Expect = 2.229e-160 Identity = 348/1116 (31.18%), Postives = 586/1116 (52.51%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTK-KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIG---RDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGV--ALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIP--NKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS-NINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVG----------------NDGNWSSSAS 3820
K YH N + + +R+ IPP SV K+E+ +SG+RDGVA A + ++ Y Q + + +EI K +HKYIIGPRG +++IL + V V++ + T+ L G LG LT+V+ K+ SV I + A WLH+FIIGK G+N+ ++ N P + + F+DE N+IVIEG PEE + L+ +V +L++R+ E+ IDP K+IIG GG +I K+R + N +I P D K ++ I G P GV+ K L + + +EN K KD++ H +IGQ+G+ +K I+D FP+V I+FP SD+++L GP+ DV++ L++ + +VE N+ V I +YHK IIG+ G IR++ + TNT+ ++P +N+ IVITGK+ D E A K + I +++NI+E + I+ LH LIG G + SL + GG V I FP + S+ V I+G K++V KA+ +L + ++ S+Y + + E + FL +KK+R+ RI S DK + ++G++ +V ++L++ M IV + + YF + R G RD + +E + +GP + ++ AK ++ ++DD +N +T +VDE+ + + + ++ +Y VQ++ P + + NS V+ + ++ ++ H +G + N + + G NKD I ITGHKD+ AKE I+ L + +V+VP +F+ +IG KG ++KL + + I IP ++NS F + +TG S + AAK+ ++E V+ +E +++R +R F+L + VP QYH ++IG +G T++ IR K+SV I P +D ++I I G++ N E A++ V+ VN+LES I + ++ID ++H RLIG++G + ++ + + V+I FP ++ +IV V G E VD C + +++ + +I ++ + + S Y+ N + + T+K+ F +R+APW G + GN SSSA+
Sbjct: 218 KMYHPFINGPRNEFASKLSKEHGVRIHIPPPSVQKDEVVVSGERDGVAAAVNKIMMVYQQKSRNCKTISVEINKSQHKYIIGPRGQTLQDILAEADVSVELPP---TDSLSETVVLRGEPGKLGQGLTIVYAKANSVVIREVSAAAWLHRFIIGKKGANIRAITSNLPRVNVEFQDEGNKIVIEGPPEEADPAQQQLELQVKELKARMDFAEVHIDPKFHKNIIGKGGQSINKLRDQYNVNIQIPTDTDKSSLIRIEGDPEGVKEAKKELEQMGQRLENEKSKDVLIEHRFHRNIIGQKGEKVKAIRDLFPKVNISFPDAKKKSDVVNLRGPKNDVEKCYKYLKQLNDDMVEKNYCIDVPIFKQYHKNIIGKGGANIRKIREETNTQIELPKEDSDNEVIVITGKKADCEKARKLIRAIEREQANIVEESVTINPQLHNQLIGAKGRLVRSLMDDYGGNVQIHFPMGSSGSDKVTIRGPKEEVEKAKDQLMQIAKQKELSSYTKELKCKAELHRFLIGRGGTTIKKVRDETGARIMFPASNDTDKETITVMGKQADVDTACKILEERIKSMENIVEIEMEIEQKHHKYF-----VARRGAVLRDIADEFGGVAVSFPRIGDESSTVRIKGPSECVEGAKSKLAELVDDLDNQVTIECLVDEQYHRTVIGQKGRNIQGVTSQYNVQVKFP------SRNNVQNSGVNKRQQQQQSR------------------------KNVKKSHKGDGD-----APANGDKGEVVENGTNEEENKDLIL----------ITGHKDRCQKAKEAILALVPISEQVDVPYKFHRYIIGQKGLGVRKLMEDYDITISIPPADQNSNF--ITITGVSSKLEAAKEGLMERVKVIEGEEEDRVLRGFTLTVDVPNQYHPQIIGRRGVTVTDIRNKHSVNIQFPDRDDEKKDQIRIIGYEKNTESARDAVMKIVNELESHISQDIHIDRKVHPRLIGTKGRAIKKIMEDFGVDIRFP------KDEDIVTVTGALEKVDECVEHILNLEEEYMQDIAEQEETKRYSHSTYQGNEKSSRS--------TNKAPFVVRDAPWHQPGSVDTNNLEEFPSLGGPSSGNKSSSAA 1264 HSP 2 Score: 92.8189 bits (229), Expect = 2.229e-160 Identity = 54/156 (34.62%), Postives = 92/156 (58.97%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSESNKLKKLLTEICAN----CGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIE 560
+T F IP EER + SE + +K +++C + +E + D SL + +SG+ + V +A++ I +QTQ ++++IPKE HR ++G GS ++LE T+T+++ P+ ST I I G+++GI ARQ IQ DE +K+ + + IE
Sbjct: 62 VTQIFHIPMEERAFKQSEFGQSQKDQSKVCIDIMRITDTMIEMSTCRDGSLSLLVSGKAENVLKARREIFNKLQTQACVTVNIPKEHHRVILGKAGSRLQKLELETATKINIPRSEKQSTEITIVGTQEGIHRARQEIQMISDEQAKLAMERLPIE 217 HSP 3 Score: 82.4185 bits (202), Expect = 7.147e-12 Identity = 115/472 (24.36%), Postives = 213/472 (45.13%), Query Frame = 2
Query: 677 ISGDRDGVAQAAS---ILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDIN-------EQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDL---SVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGV-EAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTK---ATNTKFQIPAASEENDEIVITGKQKDVEDAIKRV-CNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDL 2035
++GD + V +S L ++++T IP E+ + G K DQ+ V +DI E ++ + G +N+ A +F K ++ + ++ P H+ I+GK+GS L L + K NI R E ++ +I I G+ E + + + + + D Q++L+ + I+ + I G K+ KE I+ P + K V + G GV AV I++ + N K + + +IG +G+ L++I +V + PP D+ S+ + L G + Q I+ K +V ++V H+ IIG++G IR +T N +FQ +E ++IVI G ++ + A +++ + E ++ + + I H +IGKGG IN L++ V I+ P+ S+ + I+G + V +A+ +L +
Sbjct: 33 LAGDHENVPTTSSENQWLNPKVQRIRSTVVTQIFHIPMEERAFKQSEFGQSQK---DQSKVCIDIMRITDTMIEMSTCRDGSLSLLVSGKAENVLKARREIFNKLQTQACVTVNIPKEHHRVILGKAGSRLQKLELETATKINIP-RSEKQSTEITIVGTQEGIHRARQEI-QMISDEQAKLAMERLPIEKMYHPFINGPRNEFASKLSKEHGVRIHIPPPSVQKDEVVVSGERDGVAAAVNKIMMVYQQKSRNCKTISVEINKSQHKYIIGPRGQTLQDILAE-ADVSVELPPTDSLSETVVLRGEPGKLGQGLTIVYAKANSVV----IREVSAAAWLHRFIIGKKGANIRAITSNLPRVNVEFQ-----DEGNKIVIEGPPEEADPAQQQLELQVKELKARMDFAEVHIDPKFHKNIIGKGGQSINKLRDQYN--VNIQIPTDTDKSSLIRIEGDPEGVKEAKKELEQM 487 HSP 4 Score: 75.0998 bits (183), Expect = 1.335e-9 Identity = 51/214 (23.83%), Postives = 98/214 (45.79%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVLD--LEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFP 3529
L ++G + + A+ EI + VN+P+E + ++G G +++KL I IP + T++ + G+ E + A++EI +E ++ ER L + YH + G + E SK+ ++ VRI +P +E+ + G +D V A N+++ + + + I+ H +IG RG L + + V + P
Sbjct: 114 LLVSGKAENVLKARREIFNKLQTQACVTVNIPKEHHRVILGKAGSRLQKLELETATKINIPRSEKQSTEITIVGTQEGIHRARQEIQMISDEQAKLAMER--------LPIEKMYHPFINGPRNEFASKLSKEHGVRIHIPPPSVQKDEVVVSGERDGVAAAVNKIMMVYQQKSRNCKTISVEINKSQHKYIIGPRGQTLQDILAEADVSVELP 319
BLAST of Vigilin High density lipoprotein-binding protein vs. TrEMBL
Match: A0A4D5R9H2 (Vigilin OS=Scolopendra viridis OX=118503 GN=vigilin PE=4 SV=1) HSP 1 Score: 483.411 bits (1243), Expect = 2.103e-157 Identity = 347/1090 (31.83%), Postives = 574/1090 (52.66%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTIT--ENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSH---VSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEE-NKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGND 3796
K YH N + + + + R+++PP SV+K+EI+I+G++DGVA+A I+ + Y K Q + +E+ K +H+Y+IGPRG+ ++EIL QT V V+I E S TI L G +D LGPALTLV+ K+ S+ ++AP WLHKFIIG+ G+N+ ++ + P + + F D ++IVIEG PEEV L+K DL SR++ E+ +DP KHIIG G N+ +I++ET I+ P D V+ I G+ GV K LL + +EN K +D+I H +IG +G+ +KEI+D+F +V I F P A SD++++ GP+ DV+Q L K + L ESNF +V I + HK IIG+ G TI+++ T+TK ++PA E+D IVITGK+ +VE+A KR+ I N+I + I H +IG G I S+ E C G V + FP+ + S+ V I+G KDDV KA+ +L +L E S+Y + E ++FL +KK+R+ RI SE +D L II +++ + ++A++E+ ++ + + D++ K + F R + + +E G + P N+ S K + D + I + ++ D +Q IE Y I QKY + SK + SH V K ++ +D M E N NG + +V+ + N + + + ++ITG ++ AK+ ++ L + +EVNVP +F+ +IG KG+ +++L V+I +P + + + V+G+ +V AK+ + VE+LE+ KQ+R +R F L + V ++H K+IG KG ISKIR ++ V+I P + D N ITI G++ N E A+++++ V +LE I E ++I+ +HSRLIG++G N+ ++ ++KV+I FP R ++ + +V + G ++ V + L++ + +IND L + ++ + + +++P + GF ++ PW+ D
Sbjct: 222 KIYHPFITGSRNEIVSNIASQTNARINVPPPSVNKDEITIAGEKDGVARAKEIITKIYEDKKRKCQTVSVEVRKSQHRYVIGPRGNTLQEILAQTGVSVEIPP---FESSTETITLRGEQDKLGPALTLVYAKANSMVEAEVEAPSWLHKFIIGRKGANIRQITQDLPRVHVEFRDGTDKIVIEGPPEEVEHAKNELEKLTQDLVSRINYEEIVVDPKYHKHIIGKNGANVNRIKQETGVQISIPSDGEHNMVIRIEGSHQGVATAKQQLLDMVTKMENEKSRDLIIDHRFHRTIIGAKGEKIKEIRDKFNQVNITFADPGAKSDVVTIRGPKDDVEQCYKYLTKLNKELKESNFKIEVPIYKQLHKFIIGKAGATIKKIRDDTDTKIELPAEGNESDTIVITGKKANVEEAKKRILEIQNSHENVISDIVTIPCKFHNSVIGTKGRLIRSITEECRG-VNVFFPAEGSGSDEVTIRGPKDDVEKAKKQLMELANEKQLSSYTVEVRAKPEHHKFLIGRNGTNIKKVRDKTGARIMFPSEKDDDRELIIIAGRKDAV------EQAKAELEALIKDLDNVVEKDMSVESKYHRHFVARRGQVLR---------EITDEYGGVTVSFPRSNVNSDKVTLKGAKDCVDGAIQRIKEIVADLKQQVTIECY-------IPQKYHRTVM-----GARGSKVQNISHDYDVQIKFPDREAKEELDE----------------------MDESNQNGD--------DLQVNGDLYIDGDHNAEFLFDKPRKNDTIKITGKEENCEKAKQALLNLVPVTIEVNVPYDFHRFIIGQKGKDVRELMKEFDVNITVPPASEQSDIITVSGTPGNVEMAKQALEAKVEQLEKDKQDRILRTFQLKVEVDPRHHPKIIGRKGAVISKIRQEHEVQIQFPDKGDPEENIITITGYEKNAEAARDDIIRIVQELEEMISEDVSINHHVHSRLIGAKGRNIRKIMDQFKVDIRFP-RDSDPDLDTVV-ITGLEDQVLEAKEHLLNLEEEYMQDINDAEVLRQYTQAPSRQDDYRPR----------SENKGFVVKGGPWEQKAPD 1238 HSP 2 Score: 105.531 bits (262), Expect = 2.103e-157 Identity = 54/152 (35.53%), Postives = 91/152 (59.87%), Query Frame = 3
Query: 102 FITDTFVIPYEERRY------IDSESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIG 539
IT F +P EER+Y + + K+ +I + G +E N+ DQSL + ++G++D V +A+ I + +QTQ +++IPKE HR ++G KG +LE T+T++S P+ +D S +I+I G+++GIE A+ IQ DE +K+
Sbjct: 63 IITQVFRVPVEERKYKEINNQFGGQGGEQTKICADIMQHTGAHIEINLAKDQSLTILVTGKQDAVMKARCEIVKALQTQANATINIPKEHHRFILGLKGKKLADLEMATATKISVPRTDDNSEIIRIVGTREGIEKAKHEIQLCSDEQAKLA 214 HSP 3 Score: 128.642 bits (322), Expect = 5.238e-26 Identity = 125/526 (23.76%), Postives = 243/526 (46.20%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEA---NQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENV 2215
I ++G +D V +A I+ Q AT I IPKE H++I+G +G + ++ T+ + + R ++ S I ++G ++ + A + L + + I + P H FI G S + +V ++ N ++ + ++I I G + V E + K D + + + +E+ +++IG G +Q+I +T S+ P + + + +T+ G + ++ ++ + + + H +IG++G +++I P V + F +D I + GP ++V+ L+K LV +++ + KYHK IIG+ G + + + T + IP+ E N I I G + V A +++ ++ K N + I H +IG G +I +++ +V I F P A S+ V I+G KDDV + L L +E+ SN++ + + ++F+ A +KK+R+ +I +E + + I G+K NV
Sbjct: 118 ILVTGKQDAVMKARCEIVKALQTQANAT-----INIPKEHHRFILGLKGKKLADLEMATATKISV--PRTDDNS-EIIRIVGTREGIEKAKHEIQLCSDEQAKLAFIRLPIPKIYHPFITG-SRNEIVSNIASQTNARINVPPPSVNKDEITIAGEKDGVARAKEIITKIYEDKKRKCQTVSVEVRKSQHRYVIGPRGNTLQEILAQTGVSVEIPPFESSTETITLRGEQDKLGPALTLVYAKANSMVEAEVEAPSWLHKF---IIGRKGANIRQITQDLPRVHVEF---RDGTDKIVIEGPPEEVEHAKNELEKLTQDLVSRINYEEIVVDPKYHKHIIGKNGANVNRIKQETGVQISIPSDGEHNMVIRIEGSHQGVATAKQQLLDMVTKMENEKSRDLIIDHRFHRTIIGAKGEKIKEIRDKF-NQVNITFADPGAKSDVVTIRGPKDDVEQCYKYLTKLNKELKESNFKIEVPIYKQLHKFIIGKAGATIKKIRDDTDTKIELPAEGNESDTIVITGKKANV 627 HSP 4 Score: 89.7373 bits (221), Expect = 4.040e-14 Identity = 124/492 (25.20%), Postives = 227/492 (46.14%), Query Frame = 2
Query: 827 EILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSV-NKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLX-XXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTK---ATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXX-XLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVI-IKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEF---LFMSADLKLKKLRETYS-VRISEAEDKSHLRII---GQKENVLNTLEVLQKARSEM 2257
+I+ T ++IN L + TI + G +D + A + ++ I+ P H+FI+G G L DL + I + D+ ++I+ E IE +++ + D Q++L+ + + I + I GS + I +TN IN P + K +TI G GV K I+ K + + + + +IG +G L+EI + V + PP ++ ++ I+L G + + ++ K +VE+ +V+ P HK IIGR+G IR++T+ + +F+ + D+IVI G ++VE A + + + + I + H +IGK G +N +K+ G V I PS D + N VI I+G V A+ +L D+V T E+ + + D+ F + + K+K++R+ ++ V I+ A+ + ++ G K++V + L K E+
Sbjct: 97 DIMQHTGAHIEIN---LAKDQSLTILVTGKQDAVMKARCEIVKALQTQANATINIPKEHHRFILGLKGKKLADLEMATATKISVPRTDDNSEIIRIVGTREGIEKAKHEIQLCSDEQAKLAFIRLPIPKIYHPFITGSRNEIVSNIASQTNARINVPPPSVNKDEITIAGEKDGVARAKEIITKIYEDKKRKCQTVSVEVRKSQHRYVIGPRGNTLQEILAQTG-VSVEIPPFESSTETITLRGEQDKLGPALTLVYAKANSMVEA----EVEAPSWLHKFIIGRKGANIRQITQDLPRVHVEFR-----DGTDKIVIEGPPEEVEHAKNELEKLTQDLVSRINYEEIVVDPKYHKHIIGKNGANVNRIKQETG--VQISIPS-DGEHNMVIRIEGSHQGVATAKQQLLDMV---TKMENEKSRDLII-DHRFHRTIIGAKGEKIKEIRDKFNQVNITFADPGAKSDVVTIRGPKDDVEQCYKYLTKLNKEL 568 HSP 5 Score: 81.6481 bits (200), Expect = 1.166e-11 Identity = 56/213 (26.29%), Postives = 108/213 (50.70%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVL--DLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSR--IHSRLIGSRGANLDQLKKKYKVEIVFP 3529
+TG +D + A+ EI+K + +N+P+E + ++G KG+K+ L I +P + + + G+ E + AK EI ++ + + + + L +P YH + G++ E +S I ++ + RI+VP N +EITI G KD V +AK E++ + + + + ++++ R H +IG RG L ++ + V + P
Sbjct: 120 VTGKQDAVMKARCEIVKALQTQANATINIPKEHHRFILGLKGKKLADLEMATATKISVPRTDDNSEIIRIVGTREGIEKAKHEI--------QLCSDEQAKLAFIRLPIPKIYHPFITGSRNEIVSNIASQTNARINVPPPSVNKDEITIAGEKDGVARAK-EIITKIYEDKKRKCQTVSVEVRKSQHRYVIGPRGNTLQEILAQTGVSVEIP 323 HSP 6 Score: 71.2478 bits (173), Expect = 1.982e-8 Identity = 65/278 (23.38%), Postives = 129/278 (46.40%), Query Frame = 2
Query: 2819 EVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEIN 3649
E+++ V D I++ + I K +I L +E KL + L + P+ ++P + G++ E + + + I +P + ++ + G + V AK+ I + E+ +R Q ++++ V HR +IG +G T+ +I + V + +P +S+ IT++G +D + A V A N + +E S +H +IG +GAN+ Q+ + +V + F GT+ + ++G E V+ +EL ++ IN
Sbjct: 168 EMATATKISVPRTDDNSEIIRIVGTREGIEKAKHEIQLCSDEQAKLAFIRLPI--PKIYHPFITGSRNEIVSNIASQTNARINVPPPSVNKDEITIAGEKDGVARAKEIITKIYEDKKRKCQ-------TVSVEVRKSQHRYVIGPRGNTLQEILAQTGVSVEIPPFESSTETITLRGEQDKLGPALTLVYAKANSMVEAEVEA---PSWLHKFIIGRKGANIRQITQDLPRVHVEFR-DGTDK-----IVIEGPPEEVEHAKNELEKLTQDLVSRIN 427
BLAST of Vigilin High density lipoprotein-binding protein vs. TrEMBL
Match: A0A4W6D8F5 (Uncharacterized protein OS=Lates calcarifer OX=8187 GN=hdlbp PE=4 SV=1) HSP 1 Score: 484.952 bits (1247), Expect = 1.956e-156 Identity = 335/1116 (30.02%), Postives = 585/1116 (52.42%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVD-RANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSW 3883
K YH N + +++ R+++PP SV+K EI I+G+++ VA A +++ + Y K + +E+ K +HKY+IGP+G+ ++EILD+T V V+I ++ S T+ L G D LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG ++V V ++ V DL SR+ E+ +DP +H+IG GGVNI +I++ ++ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ +KE++D+FPEV INFP P SD++ L GPR +V++ + +QK A +VE+++S V I ++H+ IIG+ G I+++ + TNTK +PA + ++ IVITGK+ + E A R+ I ++ +NI EI + I LH LIG G + S+ E CGG V I FP+ + + V I+G ++V KA+ +L L EE ++ + E ++FL ++K+R++ RI +E +D+ + ++G +E V + L++ + +V +T+++ YF + ++ R D + V ++ S + + +G + +++AK + I++D + +T ++ ++ ++I +++ +I++ + VQI+ P +P A P E P NG E N EV + D I I+G K++ A E + L + +EV VP E + +IG KG I+K+ +V+I++P + K+ +TG + + AK+ ++E V+EL+ +++R +R+F L + V +YH K+IG KG I+ IRT++ V I P + D N ++ITI G++ A++ + A V++LE I E + +DSR+H+R+IG+RG + ++ ++KV++ FP G + N+V V G E VD D L++ M D + + ++ + S + E+ P+K GF +R APW GN+ ++S+ G P+ ++TS W
Sbjct: 229 KVYHPFITGAYNKLVGEMMQETGARINVPPPSVNKTEIVITGEKEQVALAVAMIKKIYEDKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILDRTGVSVEIPP---SDSSSETVILRGEPDRLGQALTEVYAKANSYTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTKDVQMVQSQIEAIVTDLVSRMDYAEISVDPKFHRHLIGKGGVNINRIKELHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEQRFHRAIIGQKGEKIKEVRDKFPEVIINFPDPAQKSDIVQLRGPRTEVEKCSKFMQKIVAEMVENSYSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKANCEAARNRILAIQKELANIAEIEVSIPSKLHNSLIGSKGRLVRSIMEECGG-VHIHFPTEGSGIDKVTIRGPIEEVEKAKQQLLGLAEEKQTKSHTAELRAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPTAEDKDQELITVVGTEEAVREAQKELEELIRSLDNVVEDTMSVDPKHHRYFVARRGQVLRDLADEYGGVMVSFPRTGSQSEK---VTLKGAKECVEAAKKRMQEIVEDLDAQVTIECVISQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDP-----------QAPPAEAPI--------------------------------QENG-------EANGEVKEPVDPNAPKKCDVIV----------ISGRKERCDAAVEALKALVPVTIEVEVPFELHRYIIGQKGSGIRKMMDEFEVNIQVPAPEQQSDKIAITGLANHLDRAKEGLLERVKELQAEQEDRALRSFKLTITVDPKYHPKIIGRKGAIITNIRTEHDVNIQFPEKNDENQDQITITGYEHKAIAARDAIKAIVDELEEMISEDITLDSRVHARIIGARGKGIRKIMDEFKVDLRFPQSG--AADPNLVTVTGRPELVDEAIDHLLNLEEEYMADVVENEAKMAYMRPSGGSAAAMDEQR-GPSK-------GFVVREAPW-TTGNEKAPDMTSSEDFPSFGA----PVATKTSPW 1262 HSP 2 Score: 100.523 bits (249), Expect = 1.956e-156 Identity = 54/139 (38.85%), Postives = 82/139 (58.99%), Query Frame = 3
Query: 102 FITDTFVIPYEERRYID----SESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
IT F +P EER+Y D E ++ K + +I G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G +ELE T+T++ P+ +DPS IKI G+K+G+E A+ I
Sbjct: 73 IITQVFHVPLEERKYKDINQFGEGDQAK-VCVDIMHKTGAHLELSLAKDQGLSIMVSGKLDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEI 210 HSP 3 Score: 119.783 bits (299), Expect = 3.204e-23 Identity = 129/536 (24.07%), Postives = 241/536 (44.96%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENV 2215
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++E+ +T+ + I R ++ S N I + G K+ L A + L+ + + + ++ H FI G ++G+ N+ SVNK +IVI G E+V +KK D + + + +E+ K++IG G +Q+I T S+ P D + + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP KDV V + ++ LV ++ + K+H+ +IG+ G+ I + + +IP +E+++ I I G + V++A K + + + N + I + H +IG+ G +I +++ V I FP P S+ V ++G + +V K + +V E+ ++Y + + + + +KK+RE + +I +E + + I G+K N
Sbjct: 125 IMVSGKLDAVMKARKEIVSRLQTQASAT-----VAIPKEHHRFVIGKNGEKLQELELKTATKIQI--PRPDDPS-NQIKISGTKEGLEKAKHEILLISAEQDKRAVERVNIDKVYHPFITGAYNKLVGEMMQETGARINVPPPSVNK-----------TEIVITGEKEQVALAVAMIKKIYEDKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILDRTGVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANSYTVSSVSAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTKDVQMVQSQIEAIVTDLVSRMDYAEISVDPKFHRHLIGKGGVNINRIKELHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEQRFHRAIIGQKGEKIKEVRDKF-PEVIINFPDPAQKSDIVQLRGPRTEVEKCSKFMQKIVAEMVENSYSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKANC 634 HSP 4 Score: 118.627 bits (296), Expect = 5.855e-23 Identity = 154/706 (21.81%), Postives = 308/706 (43.63%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXE-QGILEFRGPVQNIKSAKDEIIHIIDDYENTIT-ENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSST-ISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLD-LEVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFV--NKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLE 3667
+++ S V IP ++H+ +IG+ G ++EL T TK QIP + +++I I+G ++ +E A + I ++ + I K H + G + + + G R+ + P P + ++I G K+ V A + + E+ + ++ ++++ L+++ R SV I ++ S I+ G+ + + L EV KA S S++ ++ + F IG+ N+ K+ K E + + GP ++++ + +I I+ D + + + VD + +++ ++ I + ++V ++IP E N I + +K + N D++ E + G K I E+ + I+ K I ++ P+ ++E K + ++I+ V + V+VP ++F+ +IG G IKK+R I +P +NS +++TG + AA+ I+ +EL + + + + +P++ H LIG+KG + I + V I P++ S I+++TI+G + VEKAK ++L + +S E L H LIG G N+ +++ I+FP E ++ ++ V GT+E+V EL + + + + D M ++
Sbjct: 145 LQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEILLISAEQDKRAVERVNIDKVYHPFITGAYNKLVGEMMQETGARINV--PPPSVNKTEIVITGEKEQVALAVAMIKKIYEDKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILDRTGVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANS--------YTVSSVSAPSWLHR----FIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTKDVQMVQSQIEAIVTDLVSRMDYAEISVDPKFHRHLIGKGGVNINRIKELHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRME-------------NERTKDLIIEQRFHRAIIGQKGEKIKEVRDKFPEVIINFPDPAQKSDIVQLRGPRTEVE------KCSKFMQKIVAEMVENSYSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKANCEAARNRILAIQKELANIAE--------IEVSIPSKLHNSLIGSKGRLVRSIMEECGGVHIHFPTEGSGIDKVTIRGPIEEVEKAKQQLLGLAEEKQTKSHTAE-LRAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPT--AEDKDQELITVVGTEEAVREAQKELEELIRSLDNVVEDTMSVD 806 HSP 5 Score: 117.087 bits (292), Expect = 2.147e-22 Identity = 234/1059 (22.10%), Postives = 439/1059 (41.45%), Query Frame = 2
Query: 692 DGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYI-IGPRGSGIKEILDQTSVWVDIN-------EQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQL-RFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEME---IDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVE-AVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAA----SEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXX-XXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASN-----YEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSH----LRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQG---ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQM--SEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ--------DSNINE----------------------ITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDR 3655
+G + A+ +K++ +P E+ KY I G G DQ V VDI E L + +I + G D + A + ++ ++ + P H+F+IGK+G L +L + IQ+ R +D +NQI I G+ E + E K ++ + + + +E ID + I G+ + ++ +ET IN P + K + I G V AV I + +N + + +IG +G L+EI DR V + PP D+ S+ + L G P + + + K +++ V S V P H+ IIG++G + ++T+ Q+P +E D+I + G KDV+ ++ I + ++ + H LIGKGG IN +KE +VT+R P + SN + I+G V +A+ +L +L + EQ F + + K+K++R+ + I D + +++ G + V + +QK +EM V + ++S+ F ++ + G N+ K+ P E ++ G N ++A++ I+ I + N I E + +L N S+ ++ S + + V I P ++ TI + K + G+ + L P + I G NI ++ + I A +KD Q + + G ++ + A++E+ +L V++ ++V + + + +G+ ++ L + V + P S+ KV + G+ E V AAKK + E VE+L+ ++ + ++HR ++G KG I +I ++V+I P + ++ I E I I G K+ + A + A V IEV + +H +IG +G+ + ++ +++V I P ++++ + + G +DR + L++R + E DR
Sbjct: 53 EGTQETANAWTSKIRPLKSSIITQVFHVPLEERKYKDINQFGEG-----DQAKVCVDIMHKTGAHLELSLAKDQGLSIMVSGKLDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGL----EKAKHEILLISAEQDKRAVERVNIDKVYHPFITGAYNKLVGEMMQETGARINVPPPSVNKTEIVITGEKEQVALAVAMIKKIYEDKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILDRT-GVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANSYTVSS-----VSAPSWLHRFIIGKKGQNLAKITQ------QMPKVHIEFTEGEDKITLEGPTKDVQMVQSQIEAIVTDLVSRMDYAEISVDPKFHRHLIGKGGVNINRIKELH--KVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEQRF------HRAIIGQKGEKIKEVRDKFPEVIINFPDPAQKSDIVQLRGPRTEVEKCSKFMQKIVAEM---VENSYSVSVPIFKQFHRN--IIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKANCEAARNRILAIQKELAN-IAEIEVSIPSKLHNSLIGSKGRLVRSIMEECGGVHIHFPTEGSGIDKVTIRGP-IEEVEKAKQQLLGLAEEKQTKSHTAELRAKPEYHKFLI-------GKGGGNIRKVRDSTGARIIFPTAEDKD--------QELITVVGTEEAVREAQKELEELIRSLDNVVEDTMSVDPKHHRYFVARRGQVLRDLADEYGGVMVSFPRTGSQSEKVTLKGAKECVEAAKKRMQEIVEDLD--------AQVTIECVISQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDPQAPPAEAPIQENGEANGEVKEPVDPNAPKKCDVIVISGRKERCDAAVEALKALV----PVTIEV-EVPFELHRYIIGQKGSGIRKMMDEFEVNIQVP---APEQQSDKIAITGLANHLDRAKEGLLERVKELQAEQEDR 1044 HSP 6 Score: 88.5817 bits (218), Expect = 1.062e-13 Identity = 117/513 (22.81%), Postives = 235/513 (45.81%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAA-SILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFT----KSESVNIIAIDAPGWLHKFIIGKSGSNLVD-LSVNKPNIQLRFEDEANQIVI-EGSPEEVIEV--SEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKET-NCSINFPRDLTKKVVTIIGTPHGVEAVK----AILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXX-XXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEIT 2050
K +H+ K+ L + + + ++ IP N+I ISG ++G+ +A ILL + Q K + + I+ K H +I G + E++ +T +++ +N+ I + G K+ + A+ ++ K ++ IA++ HK++IG G+ L + L ++++ D +++ VI G P+ + + Y K + + S + + + IIG G N+ KI ++ I F K +T+ G V+ V+ AI+ + ++ + H LIG+ G + IK+ +V + PP + S+LI + G + V + L + A +E+ +K + I ++H+AIIG++G I+E+ K P ++++D + + G + +VE K + I + K H +IGKGG+ I ++E + I P+ +++S ++I G K + A+ ++ + +E+
Sbjct: 157 KEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEILLISAEQDKRAVERVNID--KVYHPFITGAYNKLVGEMMQETGARINVPPPSVNK---TEIVITGEKEQVALAVAMIKKIYEDKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILDRTGVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANSYTVSS------VSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDK--ITLEGPTKDVQMVQSQIEAIVTDLVSRMDYAEISVDPKFHR---HLIGKGGVNINRIKE-LHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELL-ELASRMENERTKDLIIEQRFHRAIIGQKGEKIKEVRDKFPEVIINFPDPAQKSDIVQLRGPRTEVEKCSKFMQKIVAEMVENSYSVSVPIFKQFHRNIIGKGGSNIKKIREETNTK--IDLPAENSNSEMIVITGKKANCEAARNRILAIQKELA 649
BLAST of Vigilin High density lipoprotein-binding protein vs. TrEMBL
Match: A0A4W6D887 (Uncharacterized protein OS=Lates calcarifer OX=8187 GN=hdlbp PE=4 SV=1) HSP 1 Score: 484.952 bits (1247), Expect = 2.408e-156 Identity = 335/1116 (30.02%), Postives = 585/1116 (52.42%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVD-RANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSW 3883
K YH N + +++ R+++PP SV+K EI I+G+++ VA A +++ + Y K + +E+ K +HKY+IGP+G+ ++EILD+T V V+I ++ S T+ L G D LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG ++V V ++ V DL SR+ E+ +DP +H+IG GGVNI +I++ ++ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ +KE++D+FPEV INFP P SD++ L GPR +V++ + +QK A +VE+++S V I ++H+ IIG+ G I+++ + TNTK +PA + ++ IVITGK+ + E A R+ I ++ +NI EI + I LH LIG G + S+ E CGG V I FP+ + + V I+G ++V KA+ +L L EE ++ + E ++FL ++K+R++ RI +E +D+ + ++G +E V + L++ + +V +T+++ YF + ++ R D + V ++ S + + +G + +++AK + I++D + +T ++ ++ ++I +++ +I++ + VQI+ P +P A P E P NG E N EV + D I I+G K++ A E + L + +EV VP E + +IG KG I+K+ +V+I++P + K+ +TG + + AK+ ++E V+EL+ +++R +R+F L + V +YH K+IG KG I+ IRT++ V I P + D N ++ITI G++ A++ + A V++LE I E + +DSR+H+R+IG+RG + ++ ++KV++ FP G + N+V V G E VD D L++ M D + + ++ + S + E+ P+K GF +R APW GN+ ++S+ G P+ ++TS W
Sbjct: 275 KVYHPFITGAYNKLVGEMMQETGARINVPPPSVNKTEIVITGEKEQVALAVAMIKKIYEDKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILDRTGVSVEIPP---SDSSSETVILRGEPDRLGQALTEVYAKANSYTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTKDVQMVQSQIEAIVTDLVSRMDYAEISVDPKFHRHLIGKGGVNINRIKELHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEQRFHRAIIGQKGEKIKEVRDKFPEVIINFPDPAQKSDIVQLRGPRTEVEKCSKFMQKIVAEMVENSYSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKANCEAARNRILAIQKELANIAEIEVSIPSKLHNSLIGSKGRLVRSIMEECGG-VHIHFPTEGSGIDKVTIRGPIEEVEKAKQQLLGLAEEKQTKSHTAELRAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPTAEDKDQELITVVGTEEAVREAQKELEELIRSLDNVVEDTMSVDPKHHRYFVARRGQVLRDLADEYGGVMVSFPRTGSQSEK---VTLKGAKECVEAAKKRMQEIVEDLDAQVTIECVISQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDP-----------QAPPAEAPI--------------------------------QENG-------EANGEVKEPVDPNAPKKCDVIV----------ISGRKERCDAAVEALKALVPVTIEVEVPFELHRYIIGQKGSGIRKMMDEFEVNIQVPAPEQQSDKIAITGLANHLDRAKEGLLERVKELQAEQEDRALRSFKLTITVDPKYHPKIIGRKGAIITNIRTEHDVNIQFPEKNDENQDQITITGYEHKAIAARDAIKAIVDELEEMISEDITLDSRVHARIIGARGKGIRKIMDEFKVDLRFPQSG--AADPNLVTVTGRPELVDEAIDHLLNLEEEYMADVVENEAKMAYMRPSGGSAAAMDEQR-GPSK-------GFVVREAPW-TTGNEKAPDMTSSEDFPSFGA----PVATKTSPW 1308 HSP 2 Score: 100.523 bits (249), Expect = 2.408e-156 Identity = 54/139 (38.85%), Postives = 82/139 (58.99%), Query Frame = 3
Query: 102 FITDTFVIPYEERRYID----SESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
IT F +P EER+Y D E ++ K+ +I G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G +ELE T+T++ P+ +DPS IKI G+K+G+E A+ I
Sbjct: 119 IITQVFHVPLEERKYKDINQFGEGDQ-AKVCVDIMHKTGAHLELSLAKDQGLSIMVSGKLDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEI 256 HSP 3 Score: 119.013 bits (297), Expect = 4.730e-23 Identity = 129/536 (24.07%), Postives = 241/536 (44.96%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENV 2215
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++E+ +T+ + I R ++ S N I + G K+ L A + L+ + + + ++ H FI G ++G+ N+ SVNK +IVI G E+V +KK D + + + +E+ K++IG G +Q+I T S+ P D + + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP KDV V + ++ LV ++ + K+H+ +IG+ G+ I + + +IP +E+++ I I G + V++A K + + + N + I + H +IG+ G +I +++ V I FP P S+ V ++G + +V K + +V E+ ++Y + + + + +KK+RE + +I +E + + I G+K N
Sbjct: 171 IMVSGKLDAVMKARKEIVSRLQTQASAT-----VAIPKEHHRFVIGKNGEKLQELELKTATKIQI--PRPDDPS-NQIKISGTKEGLEKAKHEILLISAEQDKRAVERVNIDKVYHPFITGAYNKLVGEMMQETGARINVPPPSVNK-----------TEIVITGEKEQVALAVAMIKKIYEDKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILDRTGVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANSYTVSSVSAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTKDVQMVQSQIEAIVTDLVSRMDYAEISVDPKFHRHLIGKGGVNINRIKELHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEQRFHRAIIGQKGEKIKEVRDKF-PEVIINFPDPAQKSDIVQLRGPRTEVEKCSKFMQKIVAEMVENSYSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKANC 680 HSP 4 Score: 119.013 bits (297), Expect = 4.893e-23 Identity = 154/706 (21.81%), Postives = 308/706 (43.63%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXE-QGILEFRGPVQNIKSAKDEIIHIIDDYENTIT-ENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSST-ISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLD-LEVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFV--NKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLE 3667
+++ S V IP ++H+ +IG+ G ++EL T TK QIP + +++I I+G ++ +E A + I ++ + I K H + G + + + G R+ + P P + ++I G K+ V A + + E+ + ++ ++++ L+++ R SV I ++ S I+ G+ + + L EV KA S S++ ++ + F IG+ N+ K+ K E + + GP ++++ + +I I+ D + + + VD + +++ ++ I + ++V ++IP E N I + +K + N D++ E + G K I E+ + I+ K I ++ P+ ++E K + ++I+ V + V+VP ++F+ +IG G IKK+R I +P +NS +++TG + AA+ I+ +EL + + + + +P++ H LIG+KG + I + V I P++ S I+++TI+G + VEKAK ++L + +S E L H LIG G N+ +++ I+FP E ++ ++ V GT+E+V EL + + + + D M ++
Sbjct: 191 LQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEILLISAEQDKRAVERVNIDKVYHPFITGAYNKLVGEMMQETGARINV--PPPSVNKTEIVITGEKEQVALAVAMIKKIYEDKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILDRTGVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANS--------YTVSSVSAPSWLHR----FIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTKDVQMVQSQIEAIVTDLVSRMDYAEISVDPKFHRHLIGKGGVNINRIKELHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRME-------------NERTKDLIIEQRFHRAIIGQKGEKIKEVRDKFPEVIINFPDPAQKSDIVQLRGPRTEVE------KCSKFMQKIVAEMVENSYSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKANCEAARNRILAIQKELANIAE--------IEVSIPSKLHNSLIGSKGRLVRSIMEECGGVHIHFPTEGSGIDKVTIRGPIEEVEKAKQQLLGLAEEKQTKSHTAE-LRAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPT--AEDKDQELITVVGTEEAVREAQKELEELIRSLDNVVEDTMSVD 852 HSP 5 Score: 117.087 bits (292), Expect = 1.839e-22 Identity = 234/1059 (22.10%), Postives = 439/1059 (41.45%), Query Frame = 2
Query: 692 DGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYI-IGPRGSGIKEILDQTSVWVDIN-------EQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQL-RFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEME---IDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVE-AVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAA----SEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXX-XXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASN-----YEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSH----LRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQG---ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQM--SEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ--------DSNINE----------------------ITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDR 3655
+G + A+ +K++ +P E+ KY I G G DQ V VDI E L + +I + G D + A + ++ ++ + P H+F+IGK+G L +L + IQ+ R +D +NQI I G+ E + E K ++ + + + +E ID + I G+ + ++ +ET IN P + K + I G V AV I + +N + + +IG +G L+EI DR V + PP D+ S+ + L G P + + + K +++ V S V P H+ IIG++G + ++T+ Q+P +E D+I + G KDV+ ++ I + ++ + H LIGKGG IN +KE +VT+R P + SN + I+G V +A+ +L +L + EQ F + + K+K++R+ + I D + +++ G + V + +QK +EM V + ++S+ F ++ + G N+ K+ P E ++ G N ++A++ I+ I + N I E + +L N S+ ++ S + + V I P ++ TI + K + G+ + L P + I G NI ++ + I A +KD Q + + G ++ + A++E+ +L V++ ++V + + + +G+ ++ L + V + P S+ KV + G+ E V AAKK + E VE+L+ ++ + ++HR ++G KG I +I ++V+I P + ++ I E I I G K+ + A + A V IEV + +H +IG +G+ + ++ +++V I P ++++ + + G +DR + L++R + E DR
Sbjct: 99 EGTQETANAWTSKIRPLKSSIITQVFHVPLEERKYKDINQFGEG-----DQAKVCVDIMHKTGAHLELSLAKDQGLSIMVSGKLDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGL----EKAKHEILLISAEQDKRAVERVNIDKVYHPFITGAYNKLVGEMMQETGARINVPPPSVNKTEIVITGEKEQVALAVAMIKKIYEDKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILDRT-GVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANSYTVSS-----VSAPSWLHRFIIGKKGQNLAKITQ------QMPKVHIEFTEGEDKITLEGPTKDVQMVQSQIEAIVTDLVSRMDYAEISVDPKFHRHLIGKGGVNINRIKELH--KVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEQRF------HRAIIGQKGEKIKEVRDKFPEVIINFPDPAQKSDIVQLRGPRTEVEKCSKFMQKIVAEM---VENSYSVSVPIFKQFHRN--IIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKANCEAARNRILAIQKELAN-IAEIEVSIPSKLHNSLIGSKGRLVRSIMEECGGVHIHFPTEGSGIDKVTIRGP-IEEVEKAKQQLLGLAEEKQTKSHTAELRAKPEYHKFLI-------GKGGGNIRKVRDSTGARIIFPTAEDKD--------QELITVVGTEEAVREAQKELEELIRSLDNVVEDTMSVDPKHHRYFVARRGQVLRDLADEYGGVMVSFPRTGSQSEKVTLKGAKECVEAAKKRMQEIVEDLD--------AQVTIECVISQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDPQAPPAEAPIQENGEANGEVKEPVDPNAPKKCDVIVISGRKERCDAAVEALKALV----PVTIEV-EVPFELHRYIIGQKGSGIRKMMDEFEVNIQVP---APEQQSDKIAITGLANHLDRAKEGLLERVKELQAEQEDR 1090 HSP 6 Score: 88.1965 bits (217), Expect = 1.461e-13 Identity = 117/513 (22.81%), Postives = 235/513 (45.81%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAA-SILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFT----KSESVNIIAIDAPGWLHKFIIGKSGSNLVD-LSVNKPNIQLRFEDEANQIVI-EGSPEEVIEV--SEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKET-NCSINFPRDLTKKVVTIIGTPHGVEAVK----AILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXX-XXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEIT 2050
K +H+ K+ L + + + ++ IP N+I ISG ++G+ +A ILL + Q K + + I+ K H +I G + E++ +T +++ +N+ I + G K+ + A+ ++ K ++ IA++ HK++IG G+ L + L ++++ D +++ VI G P+ + + Y K + + S + + + IIG G N+ KI ++ I F K +T+ G V+ V+ AI+ + ++ + H LIG+ G + IK+ +V + PP + S+LI + G + V + L + A +E+ +K + I ++H+AIIG++G I+E+ K P ++++D + + G + +VE K + I + K H +IGKGG+ I ++E + I P+ +++S ++I G K + A+ ++ + +E+
Sbjct: 203 KEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEILLISAEQDKRAVERVNID--KVYHPFITGAYNKLVGEMMQETGARINVPPPSVNK---TEIVITGEKEQVALAVAMIKKIYEDKKKNATTIAVEVKKSQHKYVIGPKGNTLQEILDRTGVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANSYTVSS------VSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDK--ITLEGPTKDVQMVQSQIEAIVTDLVSRMDYAEISVDPKFHR---HLIGKGGVNINRIKE-LHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELL-ELASRMENERTKDLIIEQRFHRAIIGQKGEKIKEVRDKFPEVIINFPDPAQKSDIVQLRGPRTEVEKCSKFMQKIVAEMVENSYSVSVPIFKQFHRNIIGKGGSNIKKIREETNTK--IDLPAENSNSEMIVITGKKANCEAARNRILAIQKELA 695
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Cavefish
Match: hdlbpa (high density lipoprotein binding protein a [Source:ZFIN;Acc:ZDB-GENE-030131-2032]) HSP 1 Score: 484.182 bits (1245), Expect = 2.227e-154 Identity = 332/1093 (30.38%), Postives = 569/1093 (52.06%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVD-RANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSS 3814
K YH + + +++ R+++PP S++K EI I+G+++ VA A +++ + Y + K + +E+ K +HKY+IGP+G+ ++EILD+T V V+I + S T+ L G D LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG ++V V ++ V DL SR+ E+ +DP +H+IG GG NI +I+ + S+ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ +KEI+D+FPEV INFP P SD++ L GPR +V++ A +QK A +VE++FS V I ++HK IIG+ G I+++ + TNTK +PA + ++ I+ITGK+ + E A R+ I ++ +NI E+ + I LH LIG G + S+ E CGG V I FP+ + + V I+G ++V KA+ +L L EE ++ + E ++FL ++K+R++ RI +E +D+ + +IG +E V + L+ + +V + + + YF + ++ R D + V ++ S ++ + +G +++AK ++ II+D + +T ++ ++ ++I ++ +I++ + VQI+ P A P E P NG E N +V + D I ++G K++ A E + L + +EV VP E + +IG KG I+K+ +V+I++P + + + +TG S + AK+ ++E V+EL+ +++R +R+F L + V +YH K+IG KG I+ IRT++ V I P + D N ++ITI G++ N A++ + A V++LE I E + +DSR+H+R+IG+RG + ++ ++KV++ FP G + N+V V G E VD D L++ M D + + ++ K S S E P+K GF +R APW A + SSS
Sbjct: 140 KVYHPFITGAYSKLVGEMMQETGARINVPPPSINKTEIVITGEKEQVALAVTLIKKVYEEKKRNATTIAVEVKKSQHKYVIGPKGNTLQEILDRTGVSVEIPPP---DSSSETVILRGEPDRLGQALTEVYAKANSYTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTKDVQMVQSQIEAIVTDLVSRMDYAEISVDPKFHRHLIGKGGANINRIKDQYKVSVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDMIIEQRFHRAIIGQKGEKIKEIRDKFPEVIINFPDPAQKSDIVQLRGPRTEVEKCAKFMQKMVAEMVENSFSVSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPAENSNSEMIIITGKKVNCEAARNRILAIQKELANITEVEVSIPSKLHNSLIGSKGRFVRSIMEECGG-VHIHFPTEGSGIDAVTIRGPAEEVEKARKQLLSLAEEKQTKSHTAELRAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPTAEDKDQELITVIGTEEAVAEAQKELEALIKSLDNVVEDNMLVDPKHHRYFVARRGQVLRDLADEYGGVMVSFPRTGSQSDK---VTLKGAKDCVEAAKKRMLEIIEDLDAQVTMECVIPQKFHRSIMGPKGARIQQITRDHNVQIKFP-----------EREDQQAAPAEAPV--------------------------------QENG-------EANGDVKEPVDPAAPKKCDVIV----------LSGRKERCEAAVEALKALVPVTIEVEVPFELHRYIIGQKGSGIRKMMDEFEVNIQVPAPDLQSDIISITGLSTHLDRAKEGLLERVKELQAEQEDRALRSFKLTITVDPKYHPKIIGRKGAIITTIRTEHDVNIQFPEKNDENQDQITITGYEQNAMSARDAIQAIVDELEEMISEDITLDSRVHARIIGARGKGIRKIMDEFKVDLRFPQSG--AADPNLVTVTGRPEMVDEAIDHLLNLEEEYMADVVGNESKMAYMKPSGTASASSDHEPRGPSK-------GFVVREAPW-ATDKAPDMSSS 1155 HSP 2 Score: 83.9593 bits (206), Expect = 2.227e-154 Identity = 43/111 (38.74%), Postives = 66/111 (59.46%), Query Frame = 3
Query: 174 KLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
K+ +I G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G +ELE T+T++ P+ D S IKI G+K+G+E A+ I
Sbjct: 11 KVCVDIMHKTGAHLELSMAKDQGLSIMVSGKLDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPEDSSNHIKISGTKEGLEKAKHEI 121 HSP 3 Score: 123.25 bits (308), Expect = 7.750e-28 Identity = 223/1007 (22.14%), Postives = 422/1007 (41.91%), Query Frame = 2
Query: 836 DQTSVWVDIN-------EQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQL-RFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEME---IDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVE-AVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAA----SEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXX-XLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLED--YEFLFMSADLKLKKLRETYSVRISEAEDKSH----LRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQG---ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQM--SEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQD------------------SNINE------------ITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDR 3655
DQ V VDI E + + +I + G D + A + ++ ++ + P H+F+IGK+G L +L + IQ+ R ED +N I I G+ E + E K ++ + + + +E ID + I G+ + ++ +ET IN P + K + I G V AV I E N + + +IG +G L+EI DR V + PPPD+ S+ + L G P + + + K +++ V S V P H+ IIG++G + ++T+ Q+P +E D+I + G KDV+ ++ I + ++ H LIGKGG IN +K+ +V++R P + SN + I+G V +A+ +L +L + E+ M +E + + K+K++R+ + I D + +++ G + V + +QK +EM V + ++S+ F K+ + G N+ K+ P E ++ G N ++A++ I+ I + N ITE + +L N S+ + S + + V I P +++ TI + K ++ + + L P + I G NI ++ + I A +KD Q + + G ++ + A++E+ L V++ + V + + + +G+ ++ L + V + P S+ KV + G+ + V AAKK ++E +E+L+ ++ +P ++HR ++G KG I +I ++V+I P ++ ++ E I + G K+ E A + A V IEV + +H +IG +G+ + ++ +++V I P +++I+ + G +DR + L++R + E DR
Sbjct: 8 DQAKVCVDIMHKTGAHLELSMAKDQGLSIMVSGKLDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPEDSSNHIKISGTKEGL----EKAKHEILLISAEQDKRAVERVNIDKVYHPFITGAYSKLVGEMMQETGARINVPPPSINKTEIVITGEKEQVALAVTLIKKVYEEKKRNATTIAVEVKKSQHKYVIGPKGNTLQEILDRT-GVSVEIPPPDSSSETVILRGEPDRLGQALTEVYAKANSYTVSS-----VSAPSWLHRFIIGKKGQNLAKITQ------QMPKVHIEFTEGEDKITLEGPTKDVQMVQSQIEAIVTDLVSRMDYAEISVDPKFHRHLIGKGGANINRIKDQY--KVSVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRM---ENERTKDMIIEQRFHRAIIGQKGEKIKEIRDKFPEVIINFPDPAQKSDIVQLRGPRTEVEKCAKFMQKMVAEM---VENSFSVSVPIFKQFHKN--IIGKGGANIKKIREETNTKIDLPAENSNSEMIIITGKKVNCEAARNRILAIQKELAN-ITEVEVSIPSKLHNSLIGSKGRFVRSIMEECGGVHIHFPTEGSGIDAVTIRGPAEEVEKARKQLLS-LAEEKQTKSHTAELRAKPEYHKFLI-------GKGGGNIRKVRDSTGARIIFPTAEDKD--------QELITVIGTEEAVAEAQKELEALIKSLDNVVEDNMLVDPKHHRYFVARRGQVLRDLADEYGGVMVSFPRTGSQSDKVTLKGAKDCVEAAKKRMLEIIEDLD--------AQVTMECVIPQKFHRSIMGPKGARIQQITRDHNVQIKFPEREDQQAAPAEAPVQENGEANGDVKEPVDPAAPKKCDVIVLSGRKERCEAAVEALKALV----PVTIEV-EVPFELHRYIIGQKGSGIRKMMDEFEVNIQVPAPDL---QSDIISITGLSTHLDRAKEGLLERVKELQAEQEDR 955 HSP 4 Score: 118.627 bits (296), Expect = 2.490e-26 Identity = 127/527 (24.10%), Postives = 242/527 (45.92%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEA---NQIVIEGSPEEVIEVSEYLKKKVFDLQSR-LSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENV 2215
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++E+ +T+ + I E S N I + G K+ L A + L+ + + + ++ H FI G + S LV + + ++ + +IVI G E+V ++ L KKV++ + R + + +E+ K++IG G +Q+I T S+ P D + + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP KDV V + ++ LV ++ + K+H+ +IG+ G I + +IP +E+++ I I G + V++A K + + + N + I + H +IG+ G +I +++ V I FP P S+ V ++G + +V K + +V E+ +++ + + ++ + +KK+RE + +I +E + + I G+K N
Sbjct: 36 IMVSGKLDAVMKARKEIVSRLQTQASAT-----VAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRP---EDSSNHIKISGTKEGLEKAKHEILLISAEQDKRAVERVNIDKVYHPFITG-AYSKLVGEMMQETGARINVPPPSINKTEIVITGEKEQVA-LAVTLIKKVYEEKKRNATTIAVEVKKSQHKYVIGPKGNTLQEILDRTGVSVEIPPPDSSSETVILRGEPDRLGQALTEVYAKANSYTVSSVSAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTKDVQMVQSQIEAIVTDLVSRMDYAEISVDPKFHRHLIGKGGANINRIKDQYKVSVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDMIIEQRFHRAIIGQKGEKIKEIRDKF-PEVIINFPDPAQKSDIVQLRGPRTEVEKCAKFMQKMVAEMVENSFSVSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPAENSNSEMIIITGKKVNC 545
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Cavefish
Match: hdlbpb (high density lipoprotein binding protein b [Source:ZFIN;Acc:ZDB-GENE-030131-6822]) HSP 1 Score: 255.373 bits (651), Expect = 2.703e-90 Identity = 174/520 (33.46%), Postives = 283/520 (54.42%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTK-KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSA 2116
K +H N + + + R+ IPP S+ K+EI I+G+++ VA A + + Y + K + +E+ K +HKYI+GP+G+ ++EI++ T V V++ L+ GS TI L G D LGPALT V+ K+ + + +F I K D S + + F D +I +EG EEV + +++ + DL +R+ E+ ID +H+IG G NI +I+++ S+ P+D + +V I G P GV+ + L+ + +EN + KD+I +IGQ+G+ +KE++D+FPEV INFP SD++ L GP+ +V++ A LQK LVES++S V I ++HK IIG+ G I++ K NTK +P + +++ IVI+GK+ + E A +R+ I +N+ E + I LH LIG G + S+ E CGG V I FP+ + + V I+G +V +A+ +L L EE +N+ E ++FL A
Sbjct: 228 KAFHPFIAGAYNRLVQELSQETGARISIPPPSLLKDEIVITGEKEAVAMAIARIRAIYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEIMESTGVSVEMPP--LDSGS-ETIILRGEPDKLGPALTQVYAKARCFMLTVCNI-----RFTIIKYTQLFSDWS---EQVHIEFTDGEERISLEGPTEEVEQAQSQIQEIIKDLVARMDYTEINIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSDRCGLVRIEGDPQGVQLARRELVDMAQRMENERTKDLIIEQRFHRTIIGQKGEKIKEVRDKFPEVIINFPDQSQKSDIVQLRGPKNEVEKCAKFLQKLLVELVESSYSISVPIFKQFHKNIIGKGGANIKK--KRPNTKIDLPTENSDSEMIVISGKKCNCEAARERILTIQRDLANLKETEVTIPAKLHNSLIGSKGCLVRSVMEECGG-VHIHFPAEGSGVDKVTIRGPAGEVERARRQLLQLAEEKQVNNFSIELNAKPEYHKFLIEEA 733 HSP 2 Score: 98.9821 bits (245), Expect = 2.703e-90 Identity = 53/137 (38.69%), Postives = 82/137 (59.85%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSES---NKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
IT F +P EERRY D+ + K+ +I G +E ++ DQ L + ++G+ D V +A+K I +QTQ ++ +PKE HR +IG G +ELE T+T+++ P+ +DPS I+I G+K+GIE AR I
Sbjct: 73 ITQVFHVPLEERRYKDNSQFGEGEEAKVCLDIMQKTGAHIELSLAKDQGLSIMVTGKLDSVMKARKEIVARLQTQASATVIMPKEHHRFVIGKNGEKLQELELKTATKIAIPRPDDPSANIRITGTKEGIEKARHEI 209 HSP 3 Score: 164.466 bits (415), Expect = 2.043e-40 Identity = 104/341 (30.50%), Postives = 182/341 (53.37%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQP-----------EETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSW 3883
+ +TG +K LA+ ++ L + ++V VP + + +IG KG I+K+ ++V+I IP + + + +TG SV A++ +++ V+EL+ +++R +R++ L L V +YH K+IG KG IS+IR + V + P + D + I I G++ NVE+AK + V L+ + + + +D R+H+R+IG+RG + +L +++KV+I FP G+ E+ + V V G E+VD+ D L++ + + L ++ + +P EE+ P K GF +R+APW+A G SSA D G P QTS+W
Sbjct: 870 ITVTGRVEKCELARIALLALVPVTVDVEVPYDLHRYIIGQKGTGIRKMMEEYEVNIWIPQPDQQSDVIKITGQEASVERARQALLDRVKELQAEQEDRALRSYKLTLSVDPKYHPKIIGRKGAVISQIRKDHDVNVQFPDKGDEQQDLIVIWGYERNVEEAKAAIEQMVAALQEMVSDDVRLDHRVHARIIGARGKAIRKLMEEFKVDIRFPQPGS--EDPDKVTVTGLPENVDKAIDHLLN--------LEEEYMLTVTETETLAAYMKPPSRVVGMGGGDEESRGPAK-------GFVVRDAPWNASGGKAPDMSSAEDFPTFGSG----VAPKQTSAW 1189 HSP 4 Score: 99.3673 bits (246), Expect = 2.068e-20 Identity = 142/655 (21.68%), Postives = 270/655 (41.22%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPI--VTETLALSIND------LTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEF---------RGPVQNIKSAKDEIIHIIDDYENTITENLI-VDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYK-----LEITGHKDKITLAKEEIIKLFVLDLE----VNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLI 3466
+++ S V +P ++H+ +IG+ G ++EL T TK IP + + I ITG ++ +E A + I ++ + + K H + G + L + G R++I P P + ++I G K+ V A ++ + EE K R+T ++ + E + H I+G K N L + EM P+ +ET+ L LT F + N+ + + S+ +EQ +EF GP + ++ A+ +I II D + I +D+R +++ + ++ I ++Y+V ++I P +S + P GV + E N D++ E + G K I E+ + I I P +++ G K+++ + + KL V +E ++VP ++F+ +IG G IKK R ++D +P +NS ++++G + AA++ I+ +L +K+ + +PA+ H LIG+KG + + + V I P++ S ++++TI+G VE+A+ ++L + + + LN H LI
Sbjct: 144 LQTQASATVIMPKEHHRFVIGKNGEKLQELELKTATKIAIPRPDDPSANIRITGTKEGIEKARHEILLISAEQDKRAVERLSLEKAFHPFIAGAYNRLVQELSQETGARISI--PPPSLLKDEIVITGEKEAVAMAIARIRAIYEE-----------------------------KKRKTTTISV-EVKKSQHKYIVGPKGNTLQEIMESTGVSVEMPPLDSGSETIILRGEPDKLGPALTQVYAKARCFMLTVCNIRFTIIKYTQLFSDWSEQVHIEFTDGEERISLEGPTEEVEQAQSQIQEIIKDLVARMDYTEINIDQRFHRHLIGKNGANINRIKEQYKVSVRI-----PQDSDRCGLVRIEGDP------QGVQLARRELVDMAQRMENERT--KDLIIEQRFHRTIIGQKGEKIKEVRDKFPEVI-------------INFPDQSQKSDIVQLRGPKNEVEKCAKFLQKLLVELVESSYSISVPIFKQFHKNIIGKGGANIKKKRPNTKID--LPTENSDSEMIVISGKKCNCEAARERILTIQRDLANLKETE--------VTIPAKLHNSLIGSKGCLVRSVMEECGGVHIHFPAEGSGVDKVTIRGPAGEVERARRQLLQLAEEKQVNNFSIELNAKPEYHKFLI 730 HSP 5 Score: 83.1889 bits (204), Expect = 1.510e-15 Identity = 64/244 (26.23%), Postives = 121/244 (49.59%), Query Frame = 2
Query: 2969 LEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNK--NSKFTKV--IVTGS----SESVVAAKKEIVEFVEELERMKQER----EIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTE--------GEEANIVKVKGTKESVDRCADELV 3616
+EV VP+ F+ A++G KG +I+++ +V IK P + ++ ++V + GS + V + +++ +E+ + R + ++ + VP HR +IG KG I K+ + V I +P D + I I G + +VE+A+ +L V +L++ L++D + H ++IG +GA + Q++K + V + FP +G E G E N+ + K E + E+V
Sbjct: 802 VEVVVPQRFHRAVMGPKGCRIQQITREQEVLIKFPEREESAGMSEVCPVENGSPSPEAGDAVPRRCDVITVTGRVEKCELARIALLALVPVTVDVEVPYDLHRYIIGQKGTGIRKMMEEYEVNIWIPQPDQQSDVIKITGQEASVERARQALLDRVKELQAEQEDRALRSYKLTLSVDPKYHPKIIGRKGAVISQIRKDHDVNVQFPDKGDEQQDLIVIWGYERNVEEAKAAIEQMVAALQEMV 1045 HSP 6 Score: 82.0333 bits (201), Expect = 4.173e-15 Identity = 69/250 (27.60%), Postives = 126/250 (50.40%), Query Frame = 2
Query: 2939 EEIIKLFVLDL---EVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIE-----VLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELV----DRANIMLDEIN 3649
+EIIK V + E+N+ + F+ LIG G I +++ ++V ++IP + + V + G + V A++E+V+ +RM+ ER + L + ++HR +IG KGE I ++R K V I+ P Q + + ++G K+ VEK F+ KL ++E + I + H +IG GAN+ KK+ +I P TE ++ ++ + G K + + + ++ D AN+ E+
Sbjct: 414 QEIIKDLVARMDYTEINIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSDRCGLVRIEGDPQGVQLARRELVDMA---QRMENER-----TKDLIIEQRFHRTIIGQKGEKIKEVRDKFPEVIINFPDQSQKSDIVQLRGPKNEVEKCAK----FLQKLLVELVESSYSISVPIFKQFHKNIIGKGGANIK--KKRPNTKIDLP---TENSDSEMIVISGKKCNCEAARERILTIQRDLANLKETEVT 646 HSP 7 Score: 79.7221 bits (195), Expect = 1.776e-14 Identity = 117/527 (22.20%), Postives = 228/527 (43.26%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLS------VNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP-RDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI-SEAEDKSHLRIIGQKENV 2215
I ++G D V +A I+ + Q AT + +PKE H+++IG G ++E+ +T+ + I R ++ S N I + G K+ + A + L+ + + + + H FI G + +LS ++ P L +DE IVI G E V ++ + + + + + +E+ K+I+G G +Q+I + T S+ P D + + + G P + + L + T++ K + D +V I F + ISL GP ++V+Q + +Q+ LV ++ I ++H+ +IG+ G I + + +IP S+ + I G + V+ A + + ++ ++ N + I + H +IG+ G +I +++ V I FP S+ V ++G K++V K L L+ E+ S+Y + + ++ + +KK R + + +E D + I G+K N
Sbjct: 124 IMVTGKLDSVMKARKEIVARLQTQASAT-----VIMPKEHHRFVIGKNGEKLQELELKTATKIAIP--RPDDPSAN-IRITGTKEGIEKARHEILLISAEQDKRAVERLSLEKAFHPFIAGAYNRLVQELSQETGARISIPPPSL-LKDE---IVITGEKEAVAMAIARIRAIYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEIMESTGVSVEMPPLDSGSETIILRGEP---DKLGPALTQVYAKARCFMLTVCNIRFTII--------KYTQLFSDWSEQVHIEF---TDGEERISLEGPTEEVEQAQSQIQEIIKDLVARMDYTEINIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSDRCGLVRIEGDPQGVQLARRELVDMAQRMENERTKDLIIEQRFHRTIIGQKGEKIKEVRDKFP-EVIINFPDQSQKSDIVQLRGPKNEVEKCAKFLQKLLVELVESSYSISVPIFKQFHKNIIGKGGANIKKKRPNTKIDLPTENSDSEMIVISGKKCNC 623 HSP 8 Score: 78.5666 bits (192), Expect = 4.546e-14 Identity = 74/313 (23.64%), Postives = 147/313 (46.96%), Query Frame = 2
Query: 761 LQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---------------LTLVFTKSESVNI---------IAIDAPGWLHKFIIGKSGSNL--------VDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQS-------RLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR--DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDA-DSDLISLIGPRKDVDQV 1573
+++ +P+ H+ ++GP+G I++I + V + E+ + G + +C P +N P+ +T K E I + ++ P LH++IIG+ G+ + V++ + +P+ Q ++ I I G V + L +V +LQ+ R +L + +DP IIG G I +IRK+ + ++ FP D + ++ I G VE KA + + + ++ D+ H + ++IG +GK ++++ + F +V I FP P + D D +++ G ++VD+
Sbjct: 802 VEVVVPQRFHRAVMGPKGCRIQQITREQEVLIKFPEREESAGM-SEVC---PVENGSPSPEAGDAVPRRCDVITVTGRVEKCELARIALLALVPVTVDVEVPYDLHRYIIGQKGTGIRKMMEEYEVNIWIPQPDQQ------SDVIKITGQEASVERARQALLDRVKELQAEQEDRALRSYKLTLSVDPKYHPKIIGRKGAVISQIRKDHDVNVQFPDKGDEQQDLIVIWGYERNVEEAKAAIEQMVAALQEMVSDDVRLDHRVHARIIGARGKAIRKLMEEF-KVDIRFPQPGSEDPDKVTVTGLPENVDKA 1103 HSP 9 Score: 78.1814 bits (191), Expect = 5.250e-14 Identity = 96/411 (23.36%), Postives = 188/411 (45.74%), Query Frame = 2
Query: 1427 LMPQ-----LIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLV-----ESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXX-XXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFR--IGRDNMCKLNVXXXXXXXXXXEQ-----GILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIP 2602
+MP+ +IG+ G+ L+E++ + +I P PD S I + G ++ +++ +H L+ + +++ + +H I G ++EL++ T + IP S DEIVITG+++ V AI R+ IYE++ +E+ K+ H ++G GN + + ES G V++ P D+ S T+I++G D KL + ++ A + F +T+ + F + E + ++ E++ L G E V ++A+S++ I+ + +A D T R IG++ + + S Q G++ G Q ++ A+ E++ + EN T++LI+++R + I ++ E+ K+ +V I P
Sbjct: 153 IMPKEHHRFVIGKNGEKLQELELKTA-TKIAIPRPDDPSANIRITGTKEGIEKA------RHEILLISAEQDKRAVERLSLEKAFHPFIAGAYNRLVQELSQETGARISIPPPSLLKDEIVITGEKEAVAMAIARIRAIYEEKKRKTTTISVEVKKSQHKYIVGPKGNTLQEIMESTG--VSVEMPPLDSGSETIILRGEPD-------KLGPALTQVYAKA--RCFMLTVCNIRFTIIKYTQLFSDWSEQVHIEFTDGEERISLE--GPTEEV-------EQAQSQIQEIIKDLVARM--DYTEINIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSDRCGLVRIEGDPQGVQLARRELVDMAQRMENERTKDLIIEQRFHRTIIGQKGEKIKEVRDKFPEVIINFP 534 HSP 10 Score: 74.7146 bits (182), Expect = 5.766e-13 Identity = 69/265 (26.04%), Postives = 131/265 (49.43%), Query Frame = 2
Query: 2873 NIKLPQYK-----LEITGHKDKITLAKEEIIKLF-------VLDLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRA 3625
++++PQ + I G + LA+ E++ + DL + + F+ +IG KGEKIK++R +V I P+++ K V + G K E+ + + L+++ E ++S+++ + Q+H+ +IG G I K R + +I +P+++S+ I I G K N E A+ +L L + + I +++H+ LIGS+G + + ++ V I FP EG + V ++G V+R +L+ A
Sbjct: 457 SVRIPQDSDRCGLVRIEGDPQGVQLARRELVDMAQRMENERTKDLIIE--QRFHRTIIGQKGEKIKEVRDKFPEVIINFPDQSQKSDIVQLRGP-------KNEVEKCAKFLQKLLVELVESSYSISVPIFKQFHKNIIGKGGANIKKKRP--NTKIDLPTENSDSEMIVISGKKCNCEAARERILTIQRDLANLKETEVTIPAKLHNSLIGSKGCLVRSVMEECGGVHIHFP---AEGSGVDKVTIRGPAGEVERARRQLLQLA 707 HSP 11 Score: 72.0182 bits (175), Expect = 4.472e-12 Identity = 44/171 (25.73%), Postives = 92/171 (53.80%), Query Frame = 2
Query: 3095 KVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCA 3604
++ + G +E V A+ +I E +++L EI ++ ++HR LIG G I++I+ + V + +P + I+G V+ A+ E++ ++E+ + L I+ R H +IG +G + +++ K+ +V I FP + ++++IV+++G K V++CA
Sbjct: 396 RISLEGPTEEVEQAQSQIQEIIKDLVARMDYTEI-------NIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSDRCGLVRIEGDPQGVQLARRELVDMAQRMENERTKDLIIEQRFHRTIIGQKGEKIKEVRDKFPEVIINFP---DQSQKSDIVQLRGPKNEVEKCA 556 HSP 12 Score: 64.3142 bits (155), Expect = 1.032e-9 Identity = 38/144 (26.39%), Postives = 74/144 (51.39%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFP 3529
++VTG +SV+ A+KEIV ++ S + +P ++HR +IG GE + ++ K + +I++P D I I G K+ +EKA++E+L + + +E L+++ H + G+ + +L ++ I P
Sbjct: 124 IMVTGKLDSVMKARKEIVARLQT-----------QASATVIMPKEHHRFVIGKNGEKLQELELKTATKIAIPRPDDPSANIRITGTKEGIEKARHEILLISAEQDKRAVERLSLEKAFHPFIAGAYNRLVQELSQETGARISIP 256 HSP 13 Score: 60.8474 bits (146), Expect = 1.030e-8 Identity = 51/213 (23.94%), Postives = 92/213 (43.19%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNV--PEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFV--NKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFP 3529
+TG D + A++EI+ V P+E + +IG GEK+++L I IP + + +TG+ E + A+ EI+ E ++ ER L + +H + G + ++ + RIS+P +EI I G K+ V A + A K ++ I V + H ++G +G L ++ + V + P
Sbjct: 126 VTGKLDSVMKARKEIVARLQTQASATVIMPKEHHRFVIGKNGEKLQELELKTATKIAIPRPDDPSANIRITGTKEGIEKARHEILLISAEQDKRAVER--------LSLEKAFHPFIAGAYNRLVQELSQETGARISIPPPSLLKDEIVITGEKEAVAMAIARIRAIYEEKKRKTTTISV-EVKKSQHKYIVGPKGNTLQEIMESTGVSVEMP 329 HSP 14 Score: 56.6102 bits (135), Expect = 1.988e-7 Identity = 61/252 (24.21%), Postives = 111/252 (44.05%), Query Frame = 2
Query: 2858 KDPISNIKLP-----QYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISK-IRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNE--------------VLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIR 3535
K P + I LP + I+G K A+E I+ + + + EV +P + + +LIG+KG ++ + V I P + S KV + G + V A++++++ EE +++ NFS+ L+ +YH+ LI K + T + P D++I I Q ++ NE + FV ES + + + R H ++G +G + Q+ ++ +V I FP R
Sbjct: 596 KRPNTKIDLPTENSDSEMIVISGKKCNCEAARERILTIQRDLANLKETEVTIPAKLHNSLIGSKGCLVRSVMEECGGVHIHFPAEGSGVDKVTIRGPAGEVERARRQLLQLAEE-------KQVNNFSIELNAKPEYHKFLIEEAVRLAQKELETLIKNLVLQPITDTDIFTIHNQHILKQIQFLINEYSIIQWNRLYILRVLCVFVQ--ESQVTVEVVVPQRFHRAVMGPKGCRIQQITREQEVLIKFPER 838 HSP 15 Score: 56.225 bits (134), Expect = 2.764e-7 Identity = 129/635 (20.31%), Postives = 263/635 (41.42%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLER-KSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFT-KSESVNI--IAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEA---NQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKVVTIIGTPHGVEAVKAIL----------LXXXXXXXXXXXXXXXFTHTLMPQ-----LIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGP----------------RKDVDQVAAILQK----KHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXL--------HTILIGKGGNRINSLKESCGGRVTIRFPSP-DADSNTVIIKGVKDDVLKAQVKLHDLV---EEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRIS----EAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALS 2290
+R+H+ + + V ++ + ++ P S + + + G ++ V + A L + ++ + + + I K+ HK IIG G+ IK+ T + D+ + + I + G K N A + T + + N+ + P LH +IG G + + + + F E +++ I G EV L + + Q +E+ P K +I V + + ET + +T + I H ++ ++ ++ L + + ++ + ++PQ ++G +G +++I R EV I FP + +S +S + P R DV V ++K + A L + V++P+ H+ IIG++G IR++ + IP +++D I ITG++ VE A + + + ++ E S L H +IG+ G I+ +++ V ++FP D + ++I G + +V +A+ + +V +E+ + + ++ + + + ++KL E + V I +ED + + G ENV ++ L E VTET L+
Sbjct: 505 QRFHRTIIGQKGEKIKEVRDKFPEVIINFPDQSQKSDIVQLRGPKNEVEKCAKFLQKLLVELVESSYSISVPIFKQFHKNIIGKGGANIKKKRPNTKI--DLPTENSDS---EMIVISGKKCNCEAARERILTIQRDLANLKETEVTIPAKLHNSLIGSKGCLVRSVMEECGGVHIHFPAEGSGVDKVTIRGPAGEVERARRQLLQLAEEKQVNNFSIELNAKPEYHKFLIEEA-VRLAQKELETLIKNLVLQPITDTDIFTIHNQHILKQIQFLINEYSIIQWNRLYILRVLCVFVQESQVTVEVVVPQRFHRAVMGPKGCRIQQIT-REQEVLIKFPERE-ESAGMSEVCPVENGSPSPEAGDAVPRRCDVITVTGRVEKCELARIALLALVPVTVDVEVPYDLHRYIIGQKGTGIRKMMEEYEVNIWIPQPDQQSDVIKITGQEASVERARQALLDRVKELQAEQEDRALRSYKLTLSVDPKYHPKIIGRKGAVISQIRKDHD--VNVQFPDKGDEQQDLIVIWGYERNVEEAKAAIEQMVAALQEMVSDDVRLDHRV----HARIIGARGKAIRKLMEEFKVDIRFPQPGSEDPDKVTVTGLPENVDKAIDHLLNLEEEYMLTVTETETLA 1125
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Cavefish
Match: mex3b (mex-3 RNA binding family member B [Source:NCBI gene;Acc:103046405]) HSP 1 Score: 60.4622 bits (145), Expect = 9.052e-9 Identity = 47/170 (27.65%), Postives = 80/170 (47.06%), Query Frame = 2
Query: 3005 LIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSL------------------ALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN-INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHS 3457
++G +G KIK LR IK P + + +VTG E V A++EI+ E ++ R +N SL + VP + ++G KG TI +I+ + I PS+D + E+T G +NV++A+ E+ A + G+IE + ++ H+
Sbjct: 73 IVGRQGCKIKALRAKTNTYIKTPVRGEE-PVFVVTGRREDVAMARREIISAAEHFSMIRASRN-KNSSLNGSTPVPGPPNLPGQTTIQVRVPYRVVGLVVGPKGATIKRIQQQTHTYIVTPSRDKEPVFEVT--GMPENVDRAREEIEAHIAMRTGGLIEFSSDENDFHA 238
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Cavefish
Match: ENSAMXT00000025356.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000037.1:51923:60658:1 gene:ENSAMXG00000024633.2 transcript:ENSAMXT00000025356.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 57.3806 bits (137), Expect = 8.739e-8 Identity = 42/154 (27.27%), Postives = 70/154 (45.45%), Query Frame = 2
Query: 3005 LIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFS--------------LALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN-INEITIQGHKDNVEKAKNEVLAFVNKLESGI 3421
++G +G KIK LR IK P + + ++TG E V A++EI+ E ++ R S + + VP + ++G KG TI +I+ + I PS+D + + EIT G N E+A+ E+ A + G+
Sbjct: 124 IVGRQGCKIKALRAKTNTYIKTPVRGEE-PVFLITGRKEDVALARREIISAAEHFSMLRASRNKLGVSFNGSPPTPLPGQTTIQVRVPYRVVGLVVGPKGSTIKRIQQQTCTYIVTPSRDRDPVFEIT--GSPGNAERAREEIEAHIAFRTGGL 274
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Cavefish
Match: igf2bp2a (insulin-like growth factor 2 mRNA binding protein 2a [Source:ZFIN;Acc:ZDB-GENE-070912-44]) HSP 1 Score: 54.299 bits (129), Expect = 2.766e-7 Identity = 46/176 (26.14%), Postives = 86/176 (48.86%), Query Frame = 2
Query: 2906 TGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKI-PNKNSKFTK--VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINE-----ITIQGHKDNVEKAKNEVLAFVNKL 3409
H + A++E++ LF+ P + ALIG KG+ IK+L IKI P +N T+ VI+TG E+ A+ I ++E + E+R + VP+ ++IG G+T+++++ +S + VP +D +E + I GH + A+ ++ + ++
Sbjct: 75 AAHTHSHSAAEQEVVYLFI-------PTQAVGALIGKKGQHIKQLAQYAGASIKIAPAENPDVTERMVIITGPPEAQFKAQGRIFGKLKEENFFSAKEEVR-LETHIKVPSSAAGRVIGKGGKTVNELQNMSSAEVIVP-RDQTPDENDEVFVKISGHFFASQTAQRKIREIIQRV 241
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Sea Lamprey
Match: hdlbpb (high density lipoprotein binding protein b [Source:ZFIN;Acc:ZDB-GENE-030131-6822]) HSP 1 Score: 337.035 bits (863), Expect = 5.245e-114 Identity = 218/686 (31.78%), Postives = 380/686 (55.39%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTK-KVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQI 2599
K +H + + +++ R++IPP SV+K+EI I+G+++ VAQAA+ + Y + K + +E+ K +HKYIIGPRG+ ++EILD T V V++ E + TI L G ++ LGPALT V+ K+ SV + + AP WLH+FIIGK G N+ +S N P + + F D ++I IEG EEV + +++ + DL R+ E+ +D +H+IG G NI +I+ + P D K ++ I G P GV LL+ +EN + KD+I H L +IGQ+G+ +KE++++F EV INFP P SD++ L GP+ +V++ A + K A L+E+N+S V I ++HK IIG+ G I+++ + TNTK +P + ++ IVITGK+ E A ++V I ++ +NI E + I LH LIG G + ++ E CGG V I FPS + S+ V I+G + +V +A +L L EE +++ + E + FL ++K+R+ RI S+ +D+ + IIG+++ V + + L + +V +++ + + +F + ++ R D + V ++ ++ + +G + ++ AK I+ I+DD E+ +T ++ ++ + + +++ ++++ Y VQI+
Sbjct: 232 KVFHPFISGPHSRIVTEMMQETGARVNIPPPSVNKDEIVITGEKEAVAQAAARIRDIYEEKKRKTTTISVEVKKSQHKYIIGPRGNTLQEILDLTGVSVEMPSV---ESNSETIILRGEQEKLGPALTQVYAKASSVACMDVYAPAWLHRFIIGKKGQNISRISQNLPKVHIEFTDGEDKITIEGPTEEVHQAQAQMEEIIKDLCGRMDFTEITVDQKFHRHLIGKSGSNINRIKDLYKVLVRIPPDHDKNNLIRIEGDPQGVHLAAKELLEMATRMENERTKDLIIEHKLHRAIIGQKGERIKEVREKFLEVIINFPDPAQKSDIVQLRGPKNEVEKCAKFMAKLVAELIENNYSISVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEMIVITGKKAHCEAAREKVLAIQKELANIKEADVTIPAKLHNSLIGAKGRLVRAIMEECGG-VHIHFPSEGSGSDKVTIRGPQPEVDRACKQLMQLAEEKQVNSFTIEVRAKPEYHRFLIGRGGANIRKVRDKTGARIVFPASDDKDQELITIIGKEDAVKDAEKELTALIKNLDDVVEDSMTVDVKHHRHFVARRCQVLRDLADEYGGVTVSLPRTGVKSDK---VVLKGAKECVEGAKKRILEIVDDLESQVTIECVIAQKYHRTVMGAKGSKVQQVTKDYDVQIKF 910 HSP 2 Score: 93.9745 bits (232), Expect = 5.245e-114 Identity = 51/141 (36.17%), Postives = 80/141 (56.74%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYID------SESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQ 509
IT F +P EER++ + E + K+ +I G +E ++ DQ L + ++G+ + V +A+K I +QTQ + IPKE HR +IG G ++LE T+T++ P+ +D S IKI G+K+GIE AR IQ
Sbjct: 74 ITQVFHVPLEERKFKEMNQTQFGEGGEQGKICLDIMQKTGAHIELSLAKDQGLSIMVTGKHEFVMKARKEIVAKLQTQATAMVAIPKEHHRYIIGRNGEKLQDLELRTATKIQIPRPDDTSNQIKITGTKEGIEKARHEIQ 214 HSP 3 Score: 146.747 bits (369), Expect = 6.776e-36 Identity = 197/896 (21.99%), Postives = 383/896 (42.75%), Query Frame = 2
Query: 1013 IIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN--GSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINE-ITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLE 3667
I+ K+G++ ++LS+ K D+ I++ G E V++ K+ V LQ++ + + + I ++IIG G +Q + T I PR D T + I GT G+E + + T + + I P + G + + E+ R+N PPP + D I + G ++ V Q AA ++ + + V++ HK IIG G T++E+ T ++P+ ++ I++ G+Q+ + A+ +V Y K S++ + + LH +IGK G I+ + ++ +V I F + + + I+G ++V +AQ ++ ++++++ +F ++ D K H +IG+ + +N ++ L K L RI D+ ++ ++ G Q + A E++ + EN T++LI++ + + I ++ E+ +K+ +++ I F P+P I EK + + L N + I + + N G NI +I E ++ I P N + ITG K A+E+++ + + + +V +P + + +LIGAKG ++ + V I P++ S KV + G V A K++++ EE +++ +F++ + +YHR LIG G I K+R K RI P+ D E ITI G +D V+ A+ E+ A + L+ + + + +D + H + R L L +Y V + P G + ++ V +KG KE V+ +++ ++D++ ++ +E
Sbjct: 108 IMQKTGAH-IELSLAK--------DQGLSIMVTGKHEFVMKAR---KEIVAKLQTQATAM-VAIPKEHHRYIIGRNGEKLQDLELRTATKIQIPRPDDTSNQIKITGTKEGIEKARHEIQLTSAEQDKRAVERISIDKVFHPFISGPHSRIVTEMMQETG-ARVNIPPPSVNKDEIVITGEKEAVAQAAARIRDIYEEKKRKTTTISVEVKKSQHKYIIGPRGNTLQEILDLTGVSVEMPSVESNSETIILRGEQEKLGPALTQV---YAKASSVACMDVYAPAWLHRFIIGKKGQNISRISQNLP-KVHIEFTDGE---DKITIEGPTEEVHQAQAQMEEIIKDLCGR------------MDFTEITVDQKF------------------HRHLIGKSGSNINRIKDLYKV---------------------------LVRIPPDH---------------DKNNLIRIEGDPQGVHLAAKELLEMATRMENERTKDLIIEHKLHRAIIGQKGERIKEVREKF-LEVIINF-PDPAQKSDIVQLRGPKNEVEK-------CAKFMAKLVAELIENNYSISVPIFKQFHKNIIGKGGANIKKIREETNTKIDL-------PTENSN--SEMIVITGKKAHCEAAREKVLAIQKELANIKEADVTIPAKLHNSLIGAKGRLVRAIMEECGGVHIHFPSEGSGSDKVTIRGPQPEVDRACKQLMQLAEE-------KQVNSFTIEVRAKPEYHRFLIGRGGANIRKVRDKTGARIVFPASDDKDQELITIIGKEDAVKDAEKELTALIKNLDDVVEDSMTVDVKHHRHFVARRCQVLRDLADEYGGVTVSLPRTGVKSDK---VVLKGAKECVEGAKKRILE----IVDDLESQVTIE 878 HSP 4 Score: 102.064 bits (253), Expect = 4.876e-22 Identity = 125/547 (22.85%), Postives = 238/547 (43.51%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA-----LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDL------SVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPI 2266
I ++G + V +A ++ Q +AT + IPKE H+YIIG G ++++ +T+ + I + + N I + G K+ + A LT +V I+ID H FI G + ++ VN P + ++IVI G E V + + ++ + + + + + +E+ K+IIG G +Q+I T S+ P + + + + G + + ++ + H +IG++G+ + I P+V I F + D I++ GP ++V Q A +++ L ++ + K+H+ +IG+ G I + +IP ++N+ I I G + V A K + + + N + I LH +IG+ G RI ++E V I FP P S+ V ++G K++V K + LV E+ +NY + + ++ + +KK+RE + +I +E + + I G+K + E + + E+ I
Sbjct: 128 IMVTGKHEFVMKARKEIVAKL-QTQATAM---VAIPKEHHRYIIGRNGEKLQDLELRTATKIQIPRP---DDTSNQIKITGTKEGIEKARHEIQLTSAEQDKRAVERISIDK--VFHPFISGPHSRIVTEMMQETGARVNIPPPSVN----KDEIVITGEKEAVAQAAARIRDIYEEKKRKTTTISVEVKKSQHKYIIGPRGNTLQEILDLTGVSVEMPSVESNSETIILRGEQEKLGPALTQVYAKASSVACMDVYAPAWLHRF---IIGKKGQNISRISQNLPKVHIEFTDGE---DKITIEGPTEEVHQAQAQMEEIIKDLCGRMDFTEITVDQKFHRHLIGKSGSNINRIKDLYKVLVRIPPDHDKNNLIRIEGDPQGVHLAAKELLEMATRMENERTKDLIIEHKLHRAIIGQKGERIKEVREKF-LEVIINFPDPAQKSDIVQLRGPKNEVEKCAKFMAKLVAELIENNYSISVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEMIVITGKKAHCEAAREKVLAIQKELANI 654 HSP 5 Score: 64.3142 bits (155), Expect = 1.863e-10 Identity = 110/462 (23.81%), Postives = 202/462 (43.72%), Query Frame = 2
Query: 803 GPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQL-RFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGV-EAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITG-------KQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLED--YEFLFMSADLKLKKLRETY 2149
G +G +I+ +T ++++ L + +I + G + + A + K ++ + P H++IIG++G L DL + IQ+ R +D +NQI I G+ +E IE + + + Q + + + ID + I G + ++ +ET +N P + K + I G V +A I E T + + +IG +G L+EI D V + P +++S+ I L R + +++ L + +A S V P H+ IIG++G I +++ K I E D+I I G Q +E+ IK +C + I + + H LIGK G+ IN +K+ +V +R P PD D N +I ++ D + +L+E T E+ + +E + + ++K++RE +
Sbjct: 99 GEQGKICLDIMQKTGAHIELS---LAKDQGLSIMVTGKHEFVMKARKEIVAKLQTQATAMVAIPKEHHRYIIGRNGEKLQDLELRTATKIQIPRPDDTSNQIKITGT-KEGIEKARHEIQLTSAEQDKRAVERISIDKVFHPFISGPHSRIVTEMMQETGARVNIPPPSVNKDEIVITGEKEAVAQAAARIRDIYEEKKRKTTTISVEVKKSQHKYIIGPRGNTLQEILD-LTGVSVEMPSVESNSETIIL---RGEQEKLGPALTQVYA-KASSVACMDVYAPAWLHRFIIGKKGQNISRISQNL-PKVHIEFTDGE-DKITIEGPTEEVHQAQAQMEEIIKDLCG------RMDFTEITVDQKFHRHLIGKSGSNINRIKDLY--KVLVRIP-PDHDKNNLI--RIEGDPQGVHLAAKELLEMATRMENERTKDLIIEHKLHRAIIGQKGERIKEVREKF 538 HSP 6 Score: 53.9138 bits (128), Expect = 2.696e-7 Identity = 40/139 (28.78%), Postives = 71/139 (51.08%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITRLV----QTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIFLHF 653
V + G K++V + K++ +LV + + IS+ I K+FH+++IG G+ K++ T+T++ P N S +I I G K E AR+ + E + I V I + + N L+ RL ++E + +HF
Sbjct: 555 VQLRGPKNEVEKCAKFMAKLVAELIENNYSISVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEMIVITGKKAHCEAAREKVLAIQKELANIKEADVTIPAKLHNSLIGAKGRLVRAIMEE--CGGVHIHF 691
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000279.1 (pep scaffold:Pmarinus_7.0:GL479126:30633:36553:-1 gene:ENSPMAG00000000249.1 transcript:ENSPMAT00000000279.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 136.346 bits (342), Expect = 1.802e-35 Identity = 88/269 (32.71%), Postives = 144/269 (53.53%), Query Frame = 2
Query: 2990 EFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN-----INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWD 3781
+F+ +IG KG I+K+ ++V+I IP + + + VTG + AK ++ +EL +++R +R F L + V ++YH K+IG KG IS+IR V I P +D + ITI G++ N E A+ +L V + E + + +D R+HSRLIG+RG + ++ +YKVEI FP ++ ++V V G E+VD D L+ N+ + + D ++ EE +S +P E K+ GF +R+APWD
Sbjct: 16 DFHRYIIGQKGTGIRKMMEEYEVNIAIPPSDMQSDVIKVTGLLPNAERAKAGLLSRAKELLAEQEDRALRGFKLTVSVDSKYHPKIIGRKGAVISQIRKDYDVNIQFPEKDGENQILPQDAITITGYERNAESAREAILKIVGEFEEMVNRDVTLDRRVHSRLIGARGKAIRKIMDEYKVEIHFP--QPTSDDPDLVTVTGLPENVDNAVDYLL---NLEEEYLMDVVESEEA-----QSYVKPSRHVEAPKAPA---KGFVVRDAPWD 271 HSP 2 Score: 81.6481 bits (200), Expect = 9.206e-17 Identity = 63/215 (29.30%), Postives = 106/215 (49.30%), Query Frame = 2
Query: 1439 LIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQV-AAILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEEN-----DEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADS-NTVIIKGVKDDVLKAQVKLHDLVEE 2044
+IGQ+G G++++ + + EV I PP D SD+I + G + ++ A +L + L E F V + KYH IIGR+G I ++ K + Q P EN D I ITG +++ E A + + I + ++ + + + +H+ LIG G I + + +V I FP P +D + V + G+ ++V A L +L EE
Sbjct: 21 IIGQKGTGIRKMMEEY-EVNIAIPPSDMQSDVIKVTGLLPNAERAKAGLLSRAKELLAEQEDRALRGFKLTVSVDSKYHPKIIGRKGAVISQIRKDYDVNIQFPEKDGENQILPQDAITITGYERNAESAREAILKIVGEFEEMVNRDVTLDRRVHSRLIGARGKAIRKIMDEY--KVEIHFPQPTSDDPDLVTVTGLPENVDNAVDYLLNLEEE 232 HSP 3 Score: 62.003 bits (149), Expect = 2.272e-10 Identity = 47/160 (29.38%), Postives = 83/160 (51.88%), Query Frame = 2
Query: 2930 LAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFT-----KVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINE-ITIQGHKDNVEKAKNEVL 3391
LA++E L L V+V +++P +IG KG I ++R + V+I+ P K+ + + +TG + +A++ I++ V E E M R+ +L V H +LIG +G+ I KI + V I P S+ + +T+ G +NV+ A + +L
Sbjct: 76 LAEQEDRALRGFKLTVSVDSKYHPKIIGRKGAVISQIRKDYDVNIQFPEKDGENQILPQDAITITGYERNAESAREAILKIVGEFEEMVN----RDVTLDRRV----HSRLIGARGKAIRKIMDEYKVEIHFPQPTSDDPDLVTVTGLPENVDNAVDYLL 227 HSP 4 Score: 55.4546 bits (132), Expect = 3.587e-8 Identity = 53/211 (25.12%), Postives = 100/211 (47.39%), Query Frame = 2
Query: 1001 LHKFIIGKSGSNLVDLSVN-KPNIQLRFEDEANQIV--------IEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRD------LTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADS-DLISLIGPRKDVDQVAAIL 1585
H++IIG+ G+ + + + NI + D + ++ E + ++ ++ L + D R +L + +D IIG G I +IRK+ + +I FP L + +TI G E+ + +LK + E +D+ + +LIG +GK +++I D + +V I+FP P +D DL+++ G ++VD L
Sbjct: 17 FHRYIIGQKGTGIRKMMEEYEVNIAIPPSDMQSDVIKVTGLLPNAERAKAGLLSRAKELLAEQEDRALRGFKLTVSVDSKYHPKIIGRKGAVISQIRKDYDVNIQFPEKDGENQILPQDAITITGYERNAESAREAILKIVGEFEEMVNRDVTLDRRVHSRLIGARGKAIRKIMDEY-KVEIHFPQPTSDDPDLVTVTGLPENVDNAVDYL 226
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Sea Lamprey
Match: BICC1 (BicC family RNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:19351]) HSP 1 Score: 51.6026 bits (122), Expect = 1.370e-6 Identity = 57/251 (22.71%), Postives = 95/251 (37.85%), Query Frame = 2
Query: 1160 VFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP------RDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKH--------------------AFLVE-------SNF--SKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVC 1807
V D +S L+M++ H+IG GG NIQ++ T C I+FP + V+I G P GVE +A F L+P + + E+ P P +A S I+ I R ++ A Q+ A L+E + F S ++ + ++H+ ++GR G+ ++ + T + Q P + I I G + V + R C
Sbjct: 51 VLDTKSNRVTLKMDVSHTEHSHVIGKGGANIQRVMANTGCHIHFPDSNRGSQAEKSNQVSIAGLPGGVEEARA------------------FIRELLPLAL------MFEV----PMTGFLQPVSEASSPAIAHIAQRHGLN--VAFKQRARLYGTVCVARGSNVSTTKEGVAMLIEHLTGSPGATFPVSSQLDVAPQHHQFMMGRNGLNVKRIVHRTGAQIQFPEPGGPHKAIHIHGNMEAV--CLARTC 269
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Yeast
Match: SCP160 (Essential RNA-binding G protein effector of mating response pathway; ligand-activated RNA-binding protein that delivers RNAs involved in polarization and perpetualizing mating signal to shmoo tip during pheromone signaling; Scp160p-mediated RNA trafficking essential for chemotropism and successful mating; mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins [Source:SGD;Acc:S000003616]) HSP 1 Score: 79.337 bits (194), Expect = 2.963e-15 Identity = 99/414 (23.91%), Postives = 193/414 (46.62%), Query Frame = 2
Query: 635 LDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDI-NEQRLNEGSPNTI--CLLGPKDNLGPALTLVFTKSES-VNIIAID--APGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKV-FDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKV--------VTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEI--KDRFPEVR---INFPPPDADSDLISLIGPRKDVDQVAAILQK--KHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCN 1810
L I + ++N++++ GD + A+AA+ + ++ ++ ++ + IP +IG +GS +++I ++ + +DI NE+ N T+ L G + NL A + +++ +II + P H +IG G+ L K N+ + F + + I G V + E LK + F++++ ++ + + + IIG G NI IR E ++F + T + I G+ ++ + + + + + H ++G G L+EI K E+R ++ P D+++ I++ GP+K V +V + K K A E++ +K + IP + A+IG GI R+L N +P + + +I ITG ++VE A K++ N
Sbjct: 596 LQIKLHTPEENQLTVRGD-EKAAKAANKIFESILNSPSSKSKMTVNIPANSVARLIGNKGSNLQQIREKFACQIDIPNEENNNASKDKTVEVTLTGLEYNLTHAKKYLAAEAKKWADIITKELIVPVKFHGSLIGPHGTYRNRLQ-EKYNVFINFPRDNEIVTIRGPSRGVNKAHEELKALLDFEMENG-HKMVINVPAEHVPRIIGKNGDNINDIRAEYGVEMDFLQKSTDPKAQETGEVELEITGSRQNIKDAAKRVESIVAEASDFVTEVLKIDHKYHKSIVGSGGHILREIISKAGGEEIRNKSVDIPNADSENKDITVQGPQKFVKKVVEEINKIVKDA---ENSVTKTIDIPAERKGALIGPGGIVRRQLESEFNINLFVPNKDDPSGKITITGAPENVEKAEKKILN 1003 HSP 2 Score: 77.7962 bits (190), Expect = 1.064e-14 Identity = 77/353 (21.81%), Postives = 170/353 (48.16%), Query Frame = 2
Query: 1043 DLSVNKPNIQLRFED-EANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKV-------VTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQI------PAASEEND-EIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGR----VTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEE 2044
D+S + ++Q++ E NQ+ + G E+ + + + + + + S S++ + I + +IG+ G N+Q+IR++ C I+ P + VT+ G + + K L + + K++I LIG G +++++ V INFP D++++++ GP + V++ L+ F +E+ + +P ++ IIG+ G I ++ + P A E + E+ ITG +++++DA KRV +I + S+ + ++I H ++G GG+ + + GG ++ P+ D+++ + ++G + V K +++ +V++
Sbjct: 588 DISEQEGHLQIKLHTPEENQLTVRGD-EKAAKAANKIFESILNSPSSKSKMTVNIPANSVARLIGNKGSNLQQIREKFACQIDIPNEENNNASKDKTVEVTLTGLEYNLTHAKKYLAAEAKKWADIITKELIVPVKFHGSLIGPHGTYRNRLQEKY-NVFINFP---RDNEIVTIRGPSRGVNKAHEELKALLDFEMENGHKMVINVPAEHVPRIIGKNGDNINDIRAEYGVEMDFLQKSTDPKAQETGEVELEITGSRQNIKDAAKRVESIVAEASDFVTEVLKIDHKYHKSIVGSGGHILREIISKAGGEEIRNKSVDIPNADSENKDITVQGPQKFVKKVVEEINKIVKD 935 HSP 3 Score: 49.2914 bits (116), Expect = 4.485e-6 Identity = 51/236 (21.61%), Postives = 117/236 (49.58%), Query Frame = 2
Query: 2888 QYKLEITGHKDKITLAKEEIIKL------FVLDLEVNVPEEFYPALIGAKGEKIKKL------RTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEAN 3559
+ +LEITG + I A + + + FV ++ + + +++ +++G+ G ++++ + IPN +S+ + V G +K + + VEE+ ++ ++ E + + + +PA+ LIG G ++ ++ ++ + VP++D +ITI G +NVEKA+ ++L + + +++ + I+ + RGA + +L+ V + F G ++AN
Sbjct: 832 EVELEITGSRQNIKDAAKRVESIVAEASDFVTEV-LKIDHKYHKSIVGSGGHILREIISKAGGEEIRNKSVDIPNADSENKDITVQG-------PQKFVKKVVEEINKIVKDAE-NSVTKTIDIPAERKGALIGPGGIVRRQLESEFNINLFVPNKDDPSGKITITGAPENVEKAEKKILNEI--IRENFDREVDVPASIY-EYVSERGAFIQKLRMDLSVNVRF---GNTSKKAN 1052
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Nematostella
Match: EDO37476 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFJ6]) HSP 1 Score: 222.246 bits (565), Expect = 6.684e-59 Identity = 243/976 (24.90%), Postives = 451/976 (46.21%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAA-SILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVF----TKSESVNIIAIDAPGWLHKFIIGKSGSNLVD-LSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKE-TNCSINFPRDLTKKVVTIIGTPHGVEAVKAIL-LXXXXXXXXXXXXXXXFTHTLMPQLIGQQG-----KGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNS---HVSAKPGEKPYMNGV-------DXXXXXXXXXXXXXP--NPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKD-------PISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNS--KFTKVI-VTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNV 3367
+ +H+ K L T+ + ++ +P D I I G ++GV +A I L + Q K ++ L I PK H +I GP I +QT ++I +N+ + + + G KD + A ++ K ++I+ HK+I+G G + + L + ++++ D ++ + ++ + V+ +V++ + + E+ + + IIG G NI+K+ ++ + F + K +T+ G P VE+ + L E I + + P +IG+ G + L I R D++SL GPR+DVD+V A L+K +A LV +N+ V I ++HK +IGR G TI+++ + T+TK ++PA ++D I+ITG + VE A +++ I + +N+ ++ + I H +IG G I S+ E CGG V+I+FP ++S+ V+I+G KDDV KA+ +L +L E +Y + E + FL ++K+RE RI ++ EDK + IIG++E V + L K+ ++ I + + +F K E+ ++ + N + +G + ++ A+ ++ I+ + E+ +T ++ + +NI + E++ +++VQI+ P P + + N H+ + P + +S+ + + L P H+P D G K N+ ++ E S IS A ++ P +N++ LE K A+ E L ++V V +++P +IG KG+ I +R + V+I+ P +++ + VI +TG S AA+ I++ V+ELE S+ L + + HR+LIG KG + K+ + V I P Q++N + + I G + +V
Sbjct: 140 REHHKFILGKGGKTLQTMELSTATKITMPRDGSDT--IKIIGTKEGVDRARHEIQLISDQQAKLAFERLAI--PKTFHPFISGPNNETANRIKEQTGAAINIPPPSVNK---DELTVAGEKDGVAQAKAMILEIYEDKKRKTTTVSIEVRKSQHKYIVGPRGGTIHEILELTGVSVEMPPSDSDSETITLRGEQDKLGVA---LTQVYEKANSVVFAEVAAPRWLHRFIIGRRGQNIRKVTQDLPKVHVEFSDE--KDSITLEGPPEQVESARESLEAFIRELIVSMAFAECNVDQKYHPHIIGKNGANGESRYLSLIHPR-------------RHDVVSLRGPREDVDKVHAYLKKLNAELVAANYCIDVPIFKQFHKNVIGRGGTTIKKIREETDTKIELPAEGSDSDVIIITGHKAQVEAAREKILAIQNELANVTQLEVHIPSKFHNSIIGAKGRLIRSVMEDCGG-VSIKFPPEGSNSDKVLIRGPKDDVEKAKKQLLELTNEKELGSYTVEIRAKPEHHRFLIGRGGASIRKVRENTGARIVFPAAKDEDKELITIIGKQEAVEAAKDELLKSIKDLDNICEGEVHVDPKWHRHFVAKRGEVL---QEIAAEFGGVVVSFPRNGVNSDRVVLKGAKECVEGARQRVMEIVQELESMVTIECVIPQEFHRNIMGAKGANVQEVTARHKVQIKFPDR-SPAGEEPVVNGDGEHLDPEAPISPRKRDIIIITGKKESAEAAKIDLLDLVPVTEQMHIPFDY--HRFVIGPKGSNVRKMMDEFSVNISIPPAKDESDSVSVIGPRANVERAMKALEA---KVAEIEAENEDRALRSFKMDVKVDRQYHPKIIGRKGQVITNIRKQYDVNIQFPPQDAPEESADVIGLTGYQHSCEAARDAILKIVKELE--------DQVSVELTIDPRIHRRLIGAKGRAVRKLMEQYKVDIRFPRQNAN-DPVVISGQEQDV 1071 HSP 2 Score: 186.808 bits (473), Expect = 7.898e-48 Identity = 241/1090 (22.11%), Postives = 476/1090 (43.67%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIG-----------KSGS--NLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP-RDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXX-XXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEIT-ASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRIS---EAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXX------XXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNS-KTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISA-------------GVALNKDPISNIKLPQYK--LEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS---NINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDG 3799
I ++G D VA+A ++L +Q++ T +++I+IP+E HK+I+G G ++ + T+ + + +GS +TI ++G K+ + A + L+ + + + P H FI G ++G+ N+ SVNK +++ + G + V + + + D + + + + +E+ K+I+G G I +I + T S+ P D + +T+ G + + + ++ + + H +IG++G+ ++++ P+V + F + D I+L GP + V+ L+ L+ S + + KYH IIG+ G +++ +D + + G ++DV+ + + + K H +IG+GG I ++E + I P+ +DS+ +II G K V A+ K+ + E+ + E H + + L + + V I E + + I G K++V +KA+ ++ + E S + + F IGR V + ++ ++ G + +++AKDE++ I D +N + VD + ++ + EI+ ++ + + F VNS + + G + + + + + + P H N G+K N+ E+ + I G + DP + I P+ + + ITG K+ AK +++ L + ++++P +++ +IG KG ++K+ V+I IP + V V G +V A K + V E+E ++R +R+F + + V QYH K+IG KG+ I+ IR + V I P QD+ + + I + G++ + E A++ +L V +LE + L ID RIH RLIG++G + +L ++YKV+I FP + + V + G ++ V+ ++L+ + + + ++EE + +Y N + +P P GF +R+APW+A G G
Sbjct: 108 IMVTGKPDTVAKARRLVL---SQLQ-TQAQIEIQIPREHHKFILGKGGKTLQTMELSTATKITMP----RDGS-DTIKIIGTKEGVDRARHEIQLISDQQAKLAFERLAIPKTFHPFISGPNNETANRIKEQTGAAINIPPPSVNK-----------DELTVAGEKDGVAQAKAMILEIYEDKKRKTTTVSIEVRKSQHKYIVGPRGGTIHEILELTGVSVEMPPSDSDSETITLRGEQDKLGVALTQVYEKANSVVFAEVAAPRWLHRF---IIGRRGQNIRKVTQDLPKVHVEF---SDEKDSITLEGPPEQVESARESLEAFIRELIVSMAFAECNVDQKYHPHIIGKNGAN-------GESRYLSLIHPRRHDVVSLRGPREDVDKVHAYLKKLNAELVAANYCIDVPIFKQFHKNVIGRGGTTIKKIREETDTK--IELPAEGSDSDVIIITGHKAQVEAAREKILAIQNELANVTQLEVHIPSKFHN-SIIGAKGRLIRSVMEDCGGVSIKFPPEGSNSDKVLIRGPKDDV-------EKAKKQLLELTNEKELGSYTVEIRAKPEHHRFLIGRGGASIRKVRENTGARIVFPAAKDEDKELITIIGKQEAVEAAKDELLKSIKDLDNICEGEVHVDPKWHRHFVAKRGEVLQEIAAEFG-GVVVSFPRNGVNSDRVVLKGAKECVEGARQRVMEIVQELESMVTIECVIPQEFH--------RNIMGAKGANVQEVTARHKVQIKFPDRSPAGEEPVVNGDGEHLDPEAPIS-PRKRDIIIITGKKESAEAAKIDLLDLVPVTEQMHIPFDYHRFVIGPKGSNVRKMMDEFSVNISIPPAKDESDSVSVIGPRANVERAMKALEAKVAEIEAENEDRALRSFKMDVKVDRQYHPKIIGRKGQVITNIRKQYDVNIQFPPQDAPEESADVIGLTGYQHSCEAARDAILKIVKELEDQVSVELTIDPRIHRRLIGAKGRAVRKLMEQYKVDIRFPRQNAN----DPVVISGQEQDVEEAKEQLLLLEEEYMQSVKE--EIEEYDAISYYMNPPSKTSPSPPPQA--GGQGFVVRDAPWNAQGGKG 1136 HSP 3 Score: 111.694 bits (278), Expect = 9.722e-25 Identity = 87/326 (26.69%), Postives = 158/326 (48.47%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPS-QDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYK-VEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHI 3856
ITGHK ++ A+E+I+ + V LEV++P +F+ ++IGAKG I+ + V IK P + S KV++ G + V AKK+++E + E+E+ ++++ + ++HR LIG G +I K+R RI P+ +D + ITI G ++ VE AK+E+L + L++ +++D + H + RG L ++ ++ V + FP G + V +KG KE C + R ++ E+ + +E + N + + HK + + S A ++ +VNG G+H+
Sbjct: 528 ITGHKAQVEAAREKILAIQNELANVTQLEVHIPSKFHNSIIGAKGRLIRSVMEDCGGVSIKFPPEGSNSDKVLIRGPKDDVEKAKKQLLE-------LTNEKELGSYTVEIRAKPEHHRFLIGRGGASIRKVRENTGARIVFPAAKDEDKELITIIGKQEAVEAAKDELLKSIKDLDNICEGEVHVDPKWHRHFVAKRGEVLQEIAAEFGGVVVSFPRNGVNSDR---VVLKGAKE----CVEGARQRVMEIVQELESMVTIECVIPQEFHRNIMGAKGANVQEVTARHKVQIKFPD------------RSPAGEEPVVNGDGEHL 827 HSP 4 Score: 88.5817 bits (218), Expect = 1.260e-17 Identity = 116/488 (23.77%), Postives = 218/488 (44.67%), Query Frame = 2
Query: 824 KEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLX-XXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTK---ATNTKFQIPAASEENDEIVITGKQKDVEDAIKRV-CNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMG 2260
++I+ +T ++++ L + TI + G D + A LV ++ ++ I I P HKFI+GK G L + ++ D ++ I I G+ +E ++ + + + + D Q++L+ + I I G +I+++T +IN P + K +T+ G GV KA++L+ + T I + ++G +G + EI + V + PP D+DS+ I+L R + D++ L + + F+ +V P H+ IIGR G IR++T+ + +F S+E D I + G + VE A + + I E ++ + + H +IGK G S +++ P + V ++G ++DV K L L E+ A+NY + + ++ + +KK+RE +I +E D + I G K V E + ++E+
Sbjct: 86 RDIMAKTGASIEVS---LGKDLSLTIMVTGKPDTVAKARRLVLSQLQTQAQIEIQIPREHHKFILGKGGKTLQTMELSTATKITMPRDGSDTIKIIGT-KEGVDRARHEIQLISDQQAKLAFERLAIPKTFHPFISGPNNETANRIKEQTGAAINIPPPSVNKDELTVAGEKDGVAQAKAMILEIYEDKKRKTTTVSIEVRKSQHKYIVGPRGGTIHEILE-LTGVSVEMPPSDSDSETITL---RGEQDKLGVALTQVYEKANSVVFA-EVAAPRWLHRFIIGRRGQNIRKVTQDLPKVHVEF-----SDEKDSITLEGPPEQVESARESLEAFIRELIVSMAFAECNVDQKYHPHIIGKNGANGESR------YLSLIHPR---RHDVVSLRGPREDVDKVHAYLKKLNAELVAANYCIDVPIFKQFHKNVIGRGGTTIKKIREETDTKIELPAEGSDSDVIIITGHKAQVEAAREKILAIQNELA 550 HSP 5 Score: 85.8853 bits (211), Expect = 8.196e-17 Identity = 48/156 (30.77%), Postives = 88/156 (56.41%), Query Frame = 3
Query: 105 ITDTFVIPYEERRY-----IDSESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEI 557
+T F +P EERRY + + +I A G +E ++ D SL + ++G+ D V +A++ + +QTQ +I + IP+E H+ ++G G + +E +T+T+++ P+ D S IKI G+K+G++ AR IQ D+ +K+ + + I
Sbjct: 55 VTQVFRVPLEERRYKLFHEATFGDERQHSICRDIMAKTGASIEVSLGKDLSLTIMVTGKPDTVAKARRLVLSQLQTQAQIEIQIPREHHKFILGKGGKTLQTMELSTATKITMPR--DGSDTIKIIGTKEGVDRARHEIQLISDQQAKLAFERLAI 208 HSP 6 Score: 49.6766 bits (117), Expect = 9.742e-6 Identity = 40/151 (26.49%), Postives = 68/151 (45.03%), Query Frame = 3
Query: 123 IPYEERRYIDSESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQ----FEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPST-LIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIE 560
IP + I +L + + E C GV ++ S V I G KD V +AKK + L + + + + E HR LIG G+ +++ NT ++ FP D LI I G ++ +E A+ + K++ + I V ++
Sbjct: 559 IPSKFHNSIIGAKGRLIRSVMEDCG--GVSIKFPPEGSNSDKVLIRGPKDDVEKAKKQLLELTNEKELGSYTVEIRAKPEHHRFLIGRGGASIRKVRENTGARIVFPAAKDEDKELITIIGKQEAVEAAKDELLKSIKDLDNICEGEVHVD 707
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Nematostella
Match: EDO32414 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SV60]) HSP 1 Score: 63.929 bits (154), Expect = 3.239e-11 Identity = 55/178 (30.90%), Postives = 87/178 (48.88%), Query Frame = 2
Query: 2975 VNVPE-EFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNF-SLA---------------LHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN-INEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIH 3454
V VP E ++G +G KIK LR IK P + +VTG E V AK+EI+ E +++ +R+ + SLA + VP + ++G KG TI +I+ + + I PS+D + E+T G +NVE A+NE+L ++ G+++ N DS H
Sbjct: 10 VPVPSSEHVAEIVGRQGCKIKALRAKTNTYIKTPVRGEDPV-FVVTGRKEDVTLAKREIISAAEHFSQIRAQRKNNSLNSLAPGPPSPSVPGQSTILVRVPYRVVGLVVGPKGATIKRIQQQTNTYIVTPSRDKEPVFEVT--GSGENVESARNEILNHISNRTGGLLDA-NDDSVFH 183
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Medaka
Match: hdlbpa (high density lipoprotein binding protein [Source:NCBI gene;Acc:101156075]) HSP 1 Score: 382.489 bits (981), Expect = 7.445e-129 Identity = 285/987 (28.88%), Postives = 501/987 (50.76%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEP----------------------VNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIK-------------LFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFT-KVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVL 3391
K YH N + + + R+++PP SV+K EI I+G+++ VA A +++ + Y + K + +E+ K +HKY++GP+G+ ++EILD+T V V+I ++ S T+ L G D LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG ++V V ++ V DL SR+ E+ +DP +H+IG GGVNI +I++ ++ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ +KE++D+FPEV INFP P SD++ L GPR +V++ + +QK A +VE++ S V I ++H+ IIG+ G I+++ + TNTK +PA + ++ IVITGK+ + E A R+ I ++ +NI E+ + I LH LIG G + S+ E CGG V I FP+ + + V I+G ++V KA+ +L L EE ++ E ++FL ++K+R++ RI E +D+ + ++G +E V + L++ + IV +T+ + YF + ++ R D + V ++ S ++ + +G + +++AK + +I+D + +T ++ ++ ++I +++ +I++ + VQI+ P +P V+ + V G K + P L I+ + S K + E+N +V G L D IS ITG + AKE +++ L L + V +++P +IG KG I +RT H V+I+ P KN + ++ +TG +AA+ I V+ELE M S + + ++ H ++IG +G+ I KI + V + P S ++ N +T+ G ++V++A + +L
Sbjct: 233 KVYHPFITGAFNKMVSEMAQDTGARINVPPPSVNKTEIVITGEKEQVALAVAMIKKVYEEKKKNTTTIAVEVKKSQHKYVVGPKGNTLQEILDRTGVSVEIPP---SDSSSETVILRGEPDRLGQALTEVYAKANSYTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTKDVQMVQSQIEAIVIDLVSRMDYAEITVDPKFHRHLIGKGGVNINRIKEMHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEQRFHRAIIGQKGEKIKEVRDKFPEVIINFPDPAHKSDIVQLRGPRTEVEKCSKFMQKMVAEMVENSHSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKVNCEAARNRILAIQKELANITEMDVSIPSKLHNSLIGSKGRLVRSIMEECGG-VHIHFPNEGSGVDKVTIRGPVEEVEKAKQQLLALAEEKQTKSHTAELHAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPTPEDKDQELITVVGTEEAVREAQKELEELIKSLDNIVEDTMNVDPKHHRYFVARRGQVLRDLADEYGGVMVSFPRTASQSDK---VSLKGAKECVEAAKKRMQEMIEDLDAQVTMECVIPQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDPQAAHSEAPAQENGEANGELKESVDPEAPKRCDVIVISGRKERCEAAIEALKALVPVTIEVDVPFELHRYIIGQKGSGIRKMMDEFEVNIQVP-----GPDLQSDKIS----------ITGLASHLDRAKEGLLERVKELQADQEDRALRSFKLTITVDPKYHPKIIGRKGAIITNIRTEHDVNIQFPEKNDENQDQITITGYEHKAIAARDAIQAIVDELEEM--------VSEDITLDSRVHARIIGARGKGIRKIMDEFKVDLRFPQSGAADPNVVTVIGRPEHVDEAIDHLL 1189 HSP 2 Score: 100.523 bits (249), Expect = 7.445e-129 Identity = 54/139 (38.85%), Postives = 82/139 (58.99%), Query Frame = 3
Query: 102 FITDTFVIPYEERRYID----SESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
IT F +P EER+Y D E ++ K + +I G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G +ELE T+T++ P+ +DPS IKI G+K+G+E A+ I
Sbjct: 77 IITQVFHVPLEERKYKDINQFGEGDQAK-VCVDIMHKTGAHLELSLAKDQGLSIMVSGKLDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEI 214 HSP 3 Score: 152.91 bits (385), Expect = 7.285e-37 Identity = 94/302 (31.13%), Postives = 167/302 (55.30%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVD-RANIMLDEINDRMQLEENK--------SSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPW 3778
I+G K++ A E + L + +EV+VP E + +IG KG I+K+ +V+I++P + + K+ +TG + + AK+ ++E V+EL+ +++R +R+F L + V +YH K+IG KG I+ IRT++ V I P + D N ++ITI G++ A++ + A V++LE + E + +DSR+H+R+IG+RG + ++ ++KV++ FP G + N+V V G E VD D L++ M D + ++ + +E+ P+K GF +R APW
Sbjct: 953 ISGRKERCEAAIEALKALVPVTIEVDVPFELHRYIIGQKGSGIRKMMDEFEVNIQVPGPDLQSDKISITGLASHLDRAKEGLLERVKELQADQEDRALRSFKLTITVDPKYHPKIIGRKGAIITNIRTEHDVNIQFPEKNDENQDQITITGYEHKAIAARDAIQAIVDELEEMVSEDITLDSRVHARIIGARGKGIRKIMDEFKVDLRFPQSG--AADPNVVTVIGRPEHVDEAIDHLLNLEEEYMADVAENEAKMAHMRPSGGSVAAGGGGGGGGASDESRGPSK-------GFVVREAPW 1245 HSP 4 Score: 142.895 bits (359), Expect = 7.679e-34 Identity = 176/771 (22.83%), Postives = 353/771 (45.78%), Query Frame = 2
Query: 1439 LIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLV-----ESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXX-XXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYS---VRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLA-LSINDLTYFQKSNELFRIGR-----DNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIPFYPEPVNSKTISNSHVSAKPGEK--PYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN--GSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPS-QDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLE 3667
+IG+ G+ L+E++ + +I P PD S+ I + G ++ +++ KH L+ + ++V I YH I G + E+ + T + +P S EIVITG+++ V A+ + +YE++ +E+ K+ H ++G GN + + + G V++ P D+ S TVI++G D + +A +++ T S+ + + F+ L K+ + + +E EDK L G ++V Q +S++ IV + ++ + ++T K + IG+ + + +++ + + + ++ G Q ++ AK E++ + EN T++LI+++R + I ++ E+ K+ +V I +P+P + I EK +M + + N + + I + + N G NI +I E ++ I A N SN ++ + ITG K A+ I+ + + +++V++P + + +LIG+KG ++ + V I PN+ S KV + G E V AK++++ EE ++ ++ + LH +YH+ LIG G I K+R RI P+ +D + IT+ G ++ V +A+ E+ + L++ + + +N+D + H + RG L L +Y V + FP ++ ++ V +KG KE C + R M+++++ ++ +E
Sbjct: 167 VIGKNGEKLQELELKTA-TKIQIPRPDDPSNQIKISGTKEGLEKA------KHEILLISAEQDKRAVERVNIDKVYHPFITGAFNKMVSEMAQDTGARINVPPPSVNKTEIVITGEKEQVALAVAMIKKVYEEKKKNTTTIAVEVKKSQHKYVVGPKGNTLQEILDRTG--VSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANSYTVSSVSAPSWL----HRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLE--GPTKDV-------QMVQSQIEAIVIDLVSRMDYAEITVDPKFHRHL-IGKGGVNINRIKEMHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEQRFHRAIIGQKGEKIKEVRDKFPEVIIN---FPDPAHKSDIVQLRGPRTEVEKCSKFMQKM---------VAEMVENSHSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLP-AEN----SNSEM----IVITGKKVNCEAARNRILAIQKELANITEMDVSIPSKLHNSLIGSKGRLVRSIMEECGGVHIHFPNEGSGVDKVTIRGPVEEVEKAKQQLLALAEE-------KQTKSHTAELHAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPTPEDKDQELITVVGTEEAVREAQKELEELIKSLDNIVEDTMNVDPKHHRYFVARRGQVLRDLADEYGGVMVSFPRTASQSDK---VSLKGAKE----CVEAAKKRMQEMIEDLDAQVTME 879 HSP 5 Score: 94.7449 bits (234), Expect = 1.657e-28 Identity = 126/531 (23.73%), Postives = 227/531 (42.75%), Query Frame = 2
Query: 656 VDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWV-----DINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGW---LHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQ---IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-----------------------------DLTKK--VVTIIGTPH----GVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVA-AILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEEN-DEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFP-SPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMT 2083
VDK ++I G + V +A LL + + ++ E HK++IG G I+++ D T + + +Q L I ++G ++ + A + +S++ I D H++ + + G L DL+ + + F A+Q + ++G+ E V + +++ + DL ++++ +E I + I+G G IQ+I ++ N I FP + K+ V+ I G +EA+KA++ ++ L +IGQ+G G++++ D F EV I P PD SD IS+ G +D+ +L++ + +F + + KYH IIGR+G I + + Q P ++EN D+I ITG + A + I ++ ++ I + +H +IG G I + + +V +RFP S AD N V + G + V +A L +L EE A E +M
Sbjct: 699 VDK--VTIRGPVEEVEKAKQQLLALAEEKQTKSHTAELHAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPTPEDKDQEL-------ITVVGTEEAVREAQKELEELIKSLDNIVEDTMNVDPKHHRYFVARRGQVLRDLADEYGGVMVSFPRTASQSDKVSLKGAKECVEAAKKRMQEMIEDLDAQVT-MECVIPQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDPQAAHSEAPAQENGEANGELKESVDPEAPKRCDVIVISGRKERCEAAIEALKALV---------PVTIEVDVPFELHRYIIGQKGSGIRKMMDEF-EVNIQVPGPDLQSDKISITGLASHLDRAKEGLLERVKELQADQEDRALRSFKLTITVDPKYHPKIIGRKGAIITNIRTEHDVNIQFPEKNDENQDQITITGYEHKAIAARDAIQAIVDELEEMVSEDITLDSRVHARIIGARGKGIRKIMDEF--KVDLRFPQSGAADPNVVTVIGRPEHVDEAIDHLLNLEEEYMADVAENEAKMA 1207 HSP 6 Score: 52.7582 bits (125), Expect = 1.657e-28 Identity = 38/122 (31.15%), Postives = 60/122 (49.18%), Query Frame = 3
Query: 288 KKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIFLHF 653
+K + +V+ +S+ I K+FHR++IG GS K++ T+T++ P N S +I I G K E AR I E + I V I S + N L+ RL ++E + +HF
Sbjct: 573 QKMVAEMVENSHSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKVNCEAARNRILAIQKELANITEMDVSIPSKLHNSLIGSKGRLVRSIMEE--CGGVHIHF 692 HSP 7 Score: 119.013 bits (297), Expect = 1.941e-26 Identity = 150/704 (21.31%), Postives = 308/704 (43.75%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXE-QGILEFRGPVQNIKSAKDEIIHIIDDYENTIT-ENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSST-ISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES-GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLE 3667
+++ S V IP ++H+ +IG+ G ++EL T TK QIP + +++I I+G ++ +E A + I ++ + I K H + G ++ + + G R+ + P P + ++I G K+ V A + + EE + ++ ++++ L+++ R SV I ++ S I+ G+ + + L EV KA S S++ ++ + F IG+ N+ K+ K E + + GP ++++ + +I I+ D + + + VD + +++ ++ I + ++V ++IP E N I + +K + N D++ E + G K I E+ + I+ +K I ++ P+ ++E ++ + ++ V+VP ++F+ +IG G IKK+R I +P +NS +++TG + AA+ I+ +EL + + + + +P++ H LIG+KG + I + V I P++ S ++++TI+G + VEKAK ++LA + ++ L+ H LIG G N+ +++ I+FP E ++ ++ V GT+E+V EL + + + + D M ++
Sbjct: 149 LQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEILLISAEQDKRAVERVNIDKVYHPFITGAFNKMVSEMAQDTGARINV--PPPSVNKTEIVITGEKEQVALAVAMIKKVYEEKKKNTTTIAVEVKKSQHKYVVGPKGNTLQEILDRTGVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANS--------YTVSSVSAPSWLHR----FIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTKDVQMVQSQIEAIVIDLVSRMDYAEITVDPKFHRHLIGKGGVNINRIKEMHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRME-------------NERTKDLIIEQRFHRAIIGQKGEKIKEVRDKFPEVIINFPDPAHKSDIVQLRGPRTEVEKCS-----KFMQKMVAEMVENSHSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKVNCEAARNRILAIQKELANITE--------MDVSIPSKLHNSLIGSKGRLVRSIMEECGGVHIHFPNEGSGVDKVTIRGPVEEVEKAKQQLLALAEEKQTKSHTAELHAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPT--PEDKDQELITVVGTEEAVREAQKELEELIKSLDNIVEDTMNVD 810 HSP 8 Score: 118.242 bits (295), Expect = 3.258e-26 Identity = 232/1029 (22.55%), Postives = 432/1029 (41.98%), Query Frame = 2
Query: 773 IPKEKHKYI-IGPRGSGIKEILDQTSVWVDIN-------EQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQL-RFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVE-AVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAA----SEENDEIVITGKQKDVEDAIKRVCNIY-XXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASN-----YEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSH----LRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQG---ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQM--SEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS------------------------------NINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDR 3655
+P E+ KY I G G DQ V VDI E L + +I + G D + A + ++ ++ + P H+F+IGK+G L +L + IQ+ R +D +NQI I G+ +E +E +++ + Q + + + ID + I G+ + ++ ++T IN P + K + I G V AV I E +NT + + ++G +G L+EI DR V + PP D+ S+ + L G P + + + K +++ V S V P H+ IIG++G + ++T+ Q+P +E D+I + G KDV+ ++ I + S + I + H LIGKGG IN +KE +VT+R P + SN + I+G V +A+ +L +L + EQ F + + K+K++R+ + I D +H +++ G + V + +QK +EM V + ++S+ F ++ + G N+ K+ P E ++ G N ++A++ I+ I + N ITE + +L N S+ ++ S + + V I P V+ TI V K + + + L P + I G NI ++ + I +KD Q + + G ++ + A++E+ +L +++ +NV + + + +G+ ++ L + V + P S+ KV + G+ E V AAKK + E +E+L+ ++ +P ++HR ++G KG I +I ++V+I P ++ + I I G K+ E A + A V IEV ++ +H +IG +G+ + ++ +++V I P + ++ + + G +DR + L++R + + DR
Sbjct: 84 VPLEERKYKDINQFGEG-----DQAKVCVDIMHKTGAHLELSLAKDQGLSIMVSGKLDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGT-KEGLEKAKHEILLISAEQDKRAVERVNIDKVYHPFITGAFNKMVSEMAQDTGARINVPPPSVNKTEIVITGEKEQVALAVAMIKKVYEEKKKNTTTIAVEVKKSQHKYVVGPKGNTLQEILDRTG-VSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANSYTVSS-----VSAPSWLHRFIIGKKGQNLAKITQ------QMPKVHIEFTEGEDKITLEGPTKDVQMVQSQIEAIVIDLVSRMDYAEITVDPKFHRHLIGKGGVNINRIKEM--HKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEQRF------HRAIIGQKGEKIKEVRDKFPEVIINFPDPAHKSDIVQLRGPRTEVEKCSKFMQKMVAEM---VENSHSVSVPIFKQFHRN--IIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKVNCEAARNRILAIQKELAN-ITEMDVSIPSKLHNSLIGSKGRLVRSIMEECGGVHIHFPNEGSGVDKVTIRGP-VEEVEKAKQQLLALAEEKQTKSHTAELHAKPEYHKFLI-------GKGGGNIRKVRDSTGARIIFPTPEDKD--------QELITVVGTEEAVREAQKELEELIKSLDNIVEDTMNVDPKHHRYFVARRGQVLRDLADEYGGVMVSFPRTASQSDKVSLKGAKECVEAAKKRMQEMIEDLD--------AQVTMECVIPQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDPQAAHSEAPAQENGEANGELKESVDPEAPKRCDVIVISGRKERCEAAIEALKALV----PVTIEV-DVPFELHRYIIGQKGSGIRKMMDEFEVNIQVPGPDLQSDK---ISITGLASHLDRAKEGLLERVKELQADQEDR 1048 HSP 9 Score: 115.161 bits (287), Expect = 2.986e-25 Identity = 139/622 (22.35%), Postives = 278/622 (44.69%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLS------VNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK-SNELF----RIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDD 2479
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++E+ +T+ + I R ++ S N I + G K+ L A + L+ + + + ++ H FI G + +++ +N P + +IVI G E+V +KK + + + + +E+ K+++G G +Q+I T S+ P D + + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP KDV V + ++ LV ++ + K+H+ +IG+ G+ I + + +IP +E+++ I I G + V++A K + + + N + I + H +IG+ G +I +++ V I FP P S+ V ++G + +V K + +V E+ +++ + + + + +KK+RE + +I +E + + I G+K N + AR+ + I E ++ D++ K N L R+ R M + + + + + RGPV+ ++ AK +++ + ++
Sbjct: 129 IMVSGKLDAVMKARKEIVSRLQTQASAT-----VAIPKEHHRFVIGKNGEKLQELELKTATKIQI--PRPDDPS-NQIKISGTKEGLEKAKHEILLISAEQDKRAVERVNIDKVYHPFITGAFNKMVSEMAQDTGARINVPPPSVN----KTEIVITGEKEQVALAVAMIKKVYEEKKKNTTTIAVEVKKSQHKYVVGPKGNTLQEILDRTGVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANSYTVSSVSAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTKDVQMVQSQIEAIVIDLVSRMDYAEITVDPKFHRHLIGKGGVNINRIKEMHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEQRFHRAIIGQKGEKIKEVRDKF-PEVIINFPDPAHKSDIVQLRGPRTEVEKCSKFMQKMVAEMVENSHSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKVNC-------EAARNRILAIQKELANITEMDVSIPSKLHNSLIGSKGRLVRSIMEECGGVHIHFPNEGSGVDKVTIRGPVEEVEKAKQQLLALAEE 724 HSP 10 Score: 84.7297 bits (208), Expect = 5.933e-16 Identity = 113/495 (22.83%), Postives = 224/495 (45.25%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAA-SILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFT----KSESVNIIAIDAPGWLHKFIIGKSGSNLVD-LSVNKPNIQLRFEDEANQIVI-EGSPEEVIEV--SEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKET-NCSINFPRDLTKKVVTIIGTPHGVEAVK----AILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXX-XXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVK 1996
K +H+ K+ L + + + ++ IP N+I ISG ++G+ +A ILL + Q K + + I+ K H +I G + E+ T +++ +N+ I + G K+ + A+ ++ K ++ IA++ HK+++G G+ L + L ++++ D +++ VI G P+ + + Y K + + S + + + IIG G N+ KI ++ I F K +T+ G V+ V+ AI++ + ++ + H LIG+ G + IK+ +V + PP + S+LI + G + V + L + A +E+ +K + I ++H+AIIG++G I+E+ K P + ++D + + G + +VE K + + + K H +IGKGG+ I ++E + I P+ +++S ++I G K
Sbjct: 161 KEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEILLISAEQDKRAVERVNID--KVYHPFITGAFNKMVSEMAQDTGARINVPPPSVNK---TEIVITGEKEQVALAVAMIKKVYEEKKKNTTTIAVEVKKSQHKYVVGPKGNTLQEILDRTGVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANSYTVSS------VSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDK--ITLEGPTKDVQMVQSQIEAIVIDLVSRMDYAEITVDPKFHR---HLIGKGGVNINRIKE-MHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLEL-ASRMENERTKDLIIEQRFHRAIIGQKGEKIKEVRDKFPEVIINFPDPAHKSDIVQLRGPRTEVEKCSKFMQKMVAEMVENSHSVSVPIFKQFHRNIIGKGGSNIKKIREETNTK--IDLPAENSNSEMIVITGKK 635 HSP 11 Score: 74.3294 bits (181), Expect = 8.738e-13 Identity = 62/280 (22.14%), Postives = 132/280 (47.14%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNK---NSKFTKVIVTGSSES-------------------VVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIV-----KVKGTKESVDRCADEL 3613
K+ + G K+ + AK+ + ++ + +E +P++F+ +++G KG +I+++ H V IK P + + ++ + E+ V++ +KE E +E +K + ++ + VP + HR +IG KG I K+ + V I VP D ++I+I G ++++AK +L V +L++ + +D + H ++IG +GA + ++ ++ V I FP + E ++ + K ++++ DEL
Sbjct: 848 KVSLKGAKECVEAAKKRMQEMIEDLDAQVTMECVIPQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDPQAAHSEAPAQENGEANGELKESVDPEAPKRCDVIVISGRKERCEAA--IEALKA---LVPVTIEVDVPFELHRYIIGQKGSGIRKMMDEFEVNIQVPGPDLQSDKISITGLASHLDRAKEGLLERVKELQADQEDRALRSFKLTITVDPKYHPKIIGRKGAIITNIRTEHDVNIQFPEKNDENQDQITITGYEHKAIAARDAIQAIVDEL 1122 HSP 12 Score: 53.5286 bits (127), Expect = 1.673e-6 Identity = 37/121 (30.58%), Postives = 59/121 (48.76%), Query Frame = 3
Query: 102 FITDTF-VIPYEERRYIDSESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQ---TQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPST 452
+ DT V P R ++ L+ L E GV V T QS V + G K+ V AKK + +++ Q + IP++FHRS++G KGS +++ + + Q+ FP+ DP
Sbjct: 802 IVEDTMNVDPKHHRYFVARRGQVLRDLADEYG---GVMVSFPRTASQSDKVSLKGAKECVEAAKKRMQEMIEDLDAQVTMECVIPQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDPQA 919
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Medaka
Match: hdlbpa (high density lipoprotein binding protein [Source:NCBI gene;Acc:101156075]) HSP 1 Score: 382.104 bits (980), Expect = 9.635e-129 Identity = 285/987 (28.88%), Postives = 501/987 (50.76%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEP----------------------VNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIK-------------LFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFT-KVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVP-SQDSNINEITIQGHKDNVEKAKNEVL 3391
K YH N + + + R+++PP SV+K EI I+G+++ VA A +++ + Y + K + +E+ K +HKY++GP+G+ ++EILD+T V V+I ++ S T+ L G D LG ALT V+ K+ S + ++ AP WLH+FIIGK G NL ++ P + + F + ++I +EG ++V V ++ V DL SR+ E+ +DP +H+IG GGVNI +I++ ++ P D K ++ I G P GV+ K LL+ +EN + KD+I +IGQ+G+ +KE++D+FPEV INFP P SD++ L GPR +V++ + +QK A +VE++ S V I ++H+ IIG+ G I+++ + TNTK +PA + ++ IVITGK+ + E A R+ I ++ +NI E+ + I LH LIG G + S+ E CGG V I FP+ + + V I+G ++V KA+ +L L EE ++ E ++FL ++K+R++ RI E +D+ + ++G +E V + L++ + IV +T+ + YF + ++ R D + V ++ S ++ + +G + +++AK + +I+D + +T ++ ++ ++I +++ +I++ + VQI+ P +P V+ + V G K + P L I+ + S K + E+N +V G L D IS ITG + AKE +++ L L + V +++P +IG KG I +RT H V+I+ P KN + ++ +TG +AA+ I V+ELE M S + + ++ H ++IG +G+ I KI + V + P S ++ N +T+ G ++V++A + +L
Sbjct: 233 KVYHPFITGAFNKMVSEMAQDTGARINVPPPSVNKTEIVITGEKEQVALAVAMIKKVYEEKKKNTTTIAVEVKKSQHKYVVGPKGNTLQEILDRTGVSVEIPP---SDSSSETVILRGEPDRLGQALTEVYAKANSYTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTKDVQMVQSQIEAIVIDLVSRMDYAEITVDPKFHRHLIGKGGVNINRIKEMHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEQRFHRAIIGQKGEKIKEVRDKFPEVIINFPDPAHKSDIVQLRGPRTEVEKCSKFMQKMVAEMVENSHSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKVNCEAARNRILAIQKELANITEMDVSIPSKLHNSLIGSKGRLVRSIMEECGG-VHIHFPNEGSGVDKVTIRGPVEEVEKAKQQLLALAEEKQTKSHTAELHAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPTPEDKDQELITVVGTEEAVREAQKELEELIKSLDNIVEDTMNVDPKHHRYFVARRGQVLRDLADEYGGVMVSFPRTASQSDK---VSLKGAKECVEAAKKRMQEMIEDLDAQVTMECVIPQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDPQAAHSEAPAQENGEANGELKESVDPEAPKRCDVIVISGRKERCEAAIEALKALVPVTIEVDVPFELHRYIIGQKGSGIRKMMDEFEVNIQVP-----GPDLQSDKIS----------ITGLASHLDRAKEGLLERVKELQADQEDRALRSFKLTITVDPKYHPKIIGRKGAIITNIRTEHDVNIQFPEKNDENQDQITITGYEHKAIAARDAIQAIVDELEEM--------VSEDITLDSRVHARIIGARGKGIRKIMDEFKVDLRFPQSGAADPNVVTVIGRPEHVDEAIDHLL 1189 HSP 2 Score: 100.523 bits (249), Expect = 9.635e-129 Identity = 54/139 (38.85%), Postives = 82/139 (58.99%), Query Frame = 3
Query: 102 FITDTFVIPYEERRYID----SESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
IT F +P EER+Y D E ++ K + +I G +E ++ DQ L + +SG+ D V +A+K I +QTQ ++ IPKE HR +IG G +ELE T+T++ P+ +DPS IKI G+K+G+E A+ I
Sbjct: 77 IITQVFHVPLEERKYKDINQFGEGDQAK-VCVDIMHKTGAHLELSLAKDQGLSIMVSGKLDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEI 214 HSP 3 Score: 159.844 bits (403), Expect = 4.943e-39 Identity = 104/337 (30.86%), Postives = 183/337 (54.30%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVD-RANIMLDEINDRMQLEENK--------SSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSW 3883
I+G K++ A E + L + +EV+VP E + +IG KG I+K+ +V+I++P + + K+ +TG + + AK+ ++E V+EL+ +++R +R+F L + V +YH K+IG KG I+ IRT++ V I P + D N ++ITI G++ A++ + A V++LE + E + +DSR+H+R+IG+RG + ++ ++KV++ FP G + N+V V G E VD D L++ M D + ++ + +E+ P+K GF +R APW A N+ S+S+ G P+P++TS W
Sbjct: 953 ISGRKERCEAAIEALKALVPVTIEVDVPFELHRYIIGQKGSGIRKMMDEFEVNIQVPGPDLQSDKISITGLASHLDRAKEGLLERVKELQADQEDRALRSFKLTITVDPKYHPKIIGRKGAIITNIRTEHDVNIQFPEKNDENQDQITITGYEHKAIAARDAIQAIVDELEEMVSEDITLDSRVHARIIGARGKGIRKIMDEFKVDLRFPQSG--AADPNVVTVIGRPEHVDEAIDHLLNLEEEYMADVAENEAKMAHMRPSGGSVAAGGGGGGGGASDESRGPSK-------GFVVREAPW-ANPNEKAPDMSSSEDFPSFGA----PVPTKTSPW 1275 HSP 4 Score: 143.28 bits (360), Expect = 7.036e-34 Identity = 176/771 (22.83%), Postives = 353/771 (45.78%), Query Frame = 2
Query: 1439 LIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLV-----ESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXX-XXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYS---VRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLA-LSINDLTYFQKSNELFRIGR-----DNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIPFYPEPVNSKTISNSHVSAKPGEK--PYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN--GSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPS-QDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLE 3667
+IG+ G+ L+E++ + +I P PD S+ I + G ++ +++ KH L+ + ++V I YH I G + E+ + T + +P S EIVITG+++ V A+ + +YE++ +E+ K+ H ++G GN + + + G V++ P D+ S TVI++G D + +A +++ T S+ + + F+ L K+ + + +E EDK L G ++V Q +S++ IV + ++ + ++T K + IG+ + + +++ + + + ++ G Q ++ AK E++ + EN T++LI+++R + I ++ E+ K+ +V I +P+P + I EK +M + + N + + I + + N G NI +I E ++ I A N SN ++ + ITG K A+ I+ + + +++V++P + + +LIG+KG ++ + V I PN+ S KV + G E V AK++++ EE ++ ++ + LH +YH+ LIG G I K+R RI P+ +D + IT+ G ++ V +A+ E+ + L++ + + +N+D + H + RG L L +Y V + FP ++ ++ V +KG KE C + R M+++++ ++ +E
Sbjct: 167 VIGKNGEKLQELELKT-ATKIQIPRPDDPSNQIKISGTKEGLEKA------KHEILLISAEQDKRAVERVNIDKVYHPFITGAFNKMVSEMAQDTGARINVPPPSVNKTEIVITGEKEQVALAVAMIKKVYEEKKKNTTTIAVEVKKSQHKYVVGPKGNTLQEILDRTG--VSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANSYTVSSVSAPSWL----HRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLE--GPTKDV-------QMVQSQIEAIVIDLVSRMDYAEITVDPKFHRHL-IGKGGVNINRIKEMHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEQRFHRAIIGQKGEKIKEVRDKFPEVIIN---FPDPAHKSDIVQLRGPRTEVEKCSKFMQKM---------VAEMVENSHSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLP-AEN----SNSEM----IVITGKKVNCEAARNRILAIQKELANITEMDVSIPSKLHNSLIGSKGRLVRSIMEECGGVHIHFPNEGSGVDKVTIRGPVEEVEKAKQQLLALAEE-------KQTKSHTAELHAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPTPEDKDQELITVVGTEEAVREAQKELEELIKSLDNIVEDTMNVDPKHHRYFVARRGQVLRDLADEYGGVMVSFPRTASQSDK---VSLKGAKE----CVEAAKKRMQEMIEDLDAQVTME 879 HSP 5 Score: 94.7449 bits (234), Expect = 2.139e-28 Identity = 123/528 (23.30%), Postives = 226/528 (42.80%), Query Frame = 2
Query: 665 NEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWV-----DINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGW---LHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQ---IVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-----------------------------DLTKK--VVTIIGTPH----GVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVA-AILQKKHAFLVES------NFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEEN-DEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFP-SPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMT 2083
++++I G + V +A LL + + ++ E HK++IG G I+++ D T + + +Q L I ++G ++ + A + +S++ I D H++ + + G L DL+ + + F A+Q + ++G+ E V + +++ + DL ++++ +E I + I+G G IQ+I ++ N I FP + K+ V+ I G +EA+KA++ ++ L +IGQ+G G++++ D F EV I P PD SD IS+ G +D+ +L++ + +F + + KYH IIGR+G I + + Q P ++EN D+I ITG + A + I ++ ++ I + +H +IG G I + + +V +RFP S AD N V + G + V +A L +L EE A E +M
Sbjct: 700 DKVTIRGPVEEVEKAKQQLLALAEEKQTKSHTAELHAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPTPEDKDQEL-------ITVVGTEEAVREAQKELEELIKSLDNIVEDTMNVDPKHHRYFVARRGQVLRDLADEYGGVMVSFPRTASQSDKVSLKGAKECVEAAKKRMQEMIEDLDAQVT-MECVIPQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDPQAAHSEAPAQENGEANGELKESVDPEAPKRCDVIVISGRKERCEAAIEALKALV---------PVTIEVDVPFELHRYIIGQKGSGIRKMMDEF-EVNIQVPGPDLQSDKISITGLASHLDRAKEGLLERVKELQADQEDRALRSFKLTITVDPKYHPKIIGRKGAIITNIRTEHDVNIQFPEKNDENQDQITITGYEHKAIAARDAIQAIVDELEEMVSEDITLDSRVHARIIGARGKGIRKIMDEF--KVDLRFPQSGAADPNVVTVIGRPEHVDEAIDHLLNLEEEYMADVAENEAKMA 1207 HSP 6 Score: 52.7582 bits (125), Expect = 2.139e-28 Identity = 38/122 (31.15%), Postives = 60/122 (49.18%), Query Frame = 3
Query: 288 KKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDIIN*LVTKAIRLFILFLKENHFDLIFLHF 653
+K + +V+ +S+ I K+FHR++IG GS K++ T+T++ P N S +I I G K E AR I E + I V I S + N L+ RL ++E + +HF
Sbjct: 573 QKMVAEMVENSHSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKVNCEAARNRILAIQKELANITEMDVSIPSKLHNSLIGSKGRLVRSIMEE--CGGVHIHF 692 HSP 7 Score: 119.398 bits (298), Expect = 1.392e-26 Identity = 154/711 (21.66%), Postives = 310/711 (43.60%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKL--RETYSVRISEAEDKSHLRII-GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXE-QGILEFRGPVQNIKSAKDEIIHIIDDYENTIT-ENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYK---LEITGHKDKITLAKEEIIKLFVLDLE----VNVP--EEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFV--NKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLE 3667
+++ S V IP ++H+ +IG+ G ++EL T TK QIP + +++I I+G ++ +E A + I ++ + I K H + G ++ + + G R+ + P P + ++I G K+ V A + + EE + ++ ++++ L+++ R SV I ++ S I+ G+ + + L EV KA S S++ ++ + F IG+ N+ K+ K E + + GP ++++ + +I I+ D + + + VD + +++ ++ I + ++V ++IP E N I + +K + N D++ E + G K I E+ + I N P +K +++ G + ++ + + K+ +E V+VP ++F+ +IG G IKK+R I +P +NS +++TG + AA+ I+ +EL + + + + +P++ H LIG+KG + I + V I P++ S ++++TI+G + VEKAK ++LA + +S E L+ H LIG G N+ +++ I+FP E ++ ++ V GT+E+V EL + + + + D M ++
Sbjct: 149 LQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEILLISAEQDKRAVERVNIDKVYHPFITGAFNKMVSEMAQDTGARINV--PPPSVNKTEIVITGEKEQVALAVAMIKKVYEEKKKNTTTIAVEVKKSQHKYVVGPKGNTLQEILDRTGVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANS--------YTVSSVSAPSWLHR----FIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTKDVQMVQSQIEAIVIDLVSRMDYAEITVDPKFHRHLIGKGGVNINRIKEMHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRME-------------NERTKDLIIEQRFHRAIIGQKGEKIKEVRDKFPEV-----------IINFPDPAHKSDIVQLRGPRTEVEKCSKFMQKMVAEMVENSHSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKVNCEAARNRILAIQKELANITE--------MDVSIPSKLHNSLIGSKGRLVRSIMEECGGVHIHFPNEGSGVDKVTIRGPVEEVEKAKQQLLALAEEKQTKSHTAE-LHAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPT--PEDKDQELITVVGTEEAVREAQKELEELIKSLDNIVEDTMNVD 810 HSP 8 Score: 117.857 bits (294), Expect = 3.726e-26 Identity = 232/1029 (22.55%), Postives = 432/1029 (41.98%), Query Frame = 2
Query: 773 IPKEKHKYI-IGPRGSGIKEILDQTSVWVDIN-------EQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPN-IQL-RFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVE-AVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIG-PRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAA----SEENDEIVITGKQKDVEDAIKRVCNIY-XXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASN-----YEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSH----LRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQG---ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQM--SEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTH-QVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDS------------------------------NINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDR 3655
+P E+ KY I G G DQ V VDI E L + +I + G D + A + ++ ++ + P H+F+IGK+G L +L + IQ+ R +D +NQI I G+ +E +E +++ + Q + + + ID + I G+ + ++ ++T IN P + K + I G V AV I E +NT + + ++G +G L+EI DR V + PP D+ S+ + L G P + + + K +++ V S V P H+ IIG++G + ++T+ Q+P +E D+I + G KDV+ ++ I + S + I + H LIGKGG IN +KE +VT+R P + SN + I+G V +A+ +L +L + EQ F + + K+K++R+ + I D +H +++ G + V + +QK +EM V + ++S+ F ++ + G N+ K+ P E ++ G N ++A++ I+ I + N ITE + +L N S+ ++ S + + V I P V+ TI V K + + + L P + I G NI ++ + I +KD Q + + G ++ + A++E+ +L +++ +NV + + + +G+ ++ L + V + P S+ KV + G+ E V AAKK + E +E+L+ ++ +P ++HR ++G KG I +I ++V+I P ++ + I I G K+ E A + A V IEV ++ +H +IG +G+ + ++ +++V I P + ++ + + G +DR + L++R + + DR
Sbjct: 84 VPLEERKYKDINQFGEG-----DQAKVCVDIMHKTGAHLELSLAKDQGLSIMVSGKLDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGT-KEGLEKAKHEILLISAEQDKRAVERVNIDKVYHPFITGAFNKMVSEMAQDTGARINVPPPSVNKTEIVITGEKEQVALAVAMIKKVYEEKKKNTTTIAVEVKKSQHKYVVGPKGNTLQEILDRT-GVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANSYTVSS-----VSAPSWLHRFIIGKKGQNLAKITQ------QMPKVHIEFTEGEDKITLEGPTKDVQMVQSQIEAIVIDLVSRMDYAEITVDPKFHRHLIGKGGVNINRIKEMH--KVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEQRF------HRAIIGQKGEKIKEVRDKFPEVIINFPDPAHKSDIVQLRGPRTEVEKCSKFMQKMVAEM---VENSHSVSVPIFKQFHRN--IIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKVNCEAARNRILAIQKELAN-ITEMDVSIPSKLHNSLIGSKGRLVRSIMEECGGVHIHFPNEGSGVDKVTIRGP-VEEVEKAKQQLLALAEEKQTKSHTAELHAKPEYHKFLI-------GKGGGNIRKVRDSTGARIIFPTPEDKD--------QELITVVGTEEAVREAQKELEELIKSLDNIVEDTMNVDPKHHRYFVARRGQVLRDLADEYGGVMVSFPRTASQSDKVSLKGAKECVEAAKKRMQEMIEDLD--------AQVTMECVIPQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDPQAAHSEAPAQENGEANGELKESVDPEAPKRCDVIVISGRKERCEAAIEALKALV----PVTIEV-DVPFELHRYIIGQKGSGIRKMMDEFEVNIQVPGPDLQSDK---ISITGLASHLDRAKEGLLERVKELQADQEDR 1048 HSP 9 Score: 115.546 bits (288), Expect = 2.391e-25 Identity = 139/622 (22.35%), Postives = 278/622 (44.69%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLS------VNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK-SNELF----RIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDD 2479
I +SG D V +A I+ + Q AT + IPKE H+++IG G ++E+ +T+ + I R ++ S N I + G K+ L A + L+ + + + ++ H FI G + +++ +N P + +IVI G E+V +KK + + + + +E+ K+++G G +Q+I T S+ P D + + V + G P + + + + + H +IG++G+ L +I + P+V I F D I+L GP KDV V + ++ LV ++ + K+H+ +IG+ G+ I + + +IP +E+++ I I G + V++A K + + + N + I + H +IG+ G +I +++ V I FP P S+ V ++G + +V K + +V E+ +++ + + + + +KK+RE + +I +E + + I G+K N + AR+ + I E ++ D++ K N L R+ R M + + + + + RGPV+ ++ AK +++ + ++
Sbjct: 129 IMVSGKLDAVMKARKEIVSRLQTQASAT-----VAIPKEHHRFVIGKNGEKLQELELKTATKIQI--PRPDDPS-NQIKISGTKEGLEKAKHEILLISAEQDKRAVERVNIDKVYHPFITGAFNKMVSEMAQDTGARINVPPPSVN----KTEIVITGEKEQVALAVAMIKKVYEEKKKNTTTIAVEVKKSQHKYVVGPKGNTLQEILDRTGVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANSYTVSSVSAPSWLHRF---IIGKKGQNLAKITQQMPKVHIEF---TEGEDKITLEGPTKDVQMVQSQIEAIVIDLVSRMDYAEITVDPKFHRHLIGKGGVNINRIKEMHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEQRFHRAIIGQKGEKIKEVRDKF-PEVIINFPDPAHKSDIVQLRGPRTEVEKCSKFMQKMVAEMVENSHSVSVPIFKQFHRNIIGKGGSNIKKIREETNTKIDLPAENSNSEMIVITGKKVNC-------EAARNRILAIQKELANITEMDVSIPSKLHNSLIGSKGRLVRSIMEECGGVHIHFPNEGSGVDKVTIRGPVEEVEKAKQQLLALAEE 724 HSP 10 Score: 84.7297 bits (208), Expect = 5.828e-16 Identity = 113/495 (22.83%), Postives = 224/495 (45.25%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAA-SILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFT----KSESVNIIAIDAPGWLHKFIIGKSGSNLVD-LSVNKPNIQLRFEDEANQIVI-EGSPEEVIEV--SEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKET-NCSINFPRDLTKKVVTIIGTPHGVEAVK----AILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELT-KATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXX-XXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVK 1996
K +H+ K+ L + + + ++ IP N+I ISG ++G+ +A ILL + Q K + + I+ K H +I G + E+ T +++ +N+ I + G K+ + A+ ++ K ++ IA++ HK+++G G+ L + L ++++ D +++ VI G P+ + + Y K + + S + + + IIG G N+ KI ++ I F K +T+ G V+ V+ AI++ + ++ + H LIG+ G + IK+ +V + PP + S+LI + G + V + L + A +E+ +K + I ++H+AIIG++G I+E+ K P + ++D + + G + +VE K + + + K H +IGKGG+ I ++E + I P+ +++S ++I G K
Sbjct: 161 KEHHRFVIGKNGEKLQELELKTATKIQIPRPDDPSNQIKISGTKEGLEKAKHEILLISAEQDKRAVERVNID--KVYHPFITGAFNKMVSEMAQDTGARINVPPPSVNK---TEIVITGEKEQVALAVAMIKKVYEEKKKNTTTIAVEVKKSQHKYVVGPKGNTLQEILDRTGVSVEIPPSDSSSETVILRGEPDRLGQALTEVYAKANSYTVSS------VSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDK--ITLEGPTKDVQMVQSQIEAIVIDLVSRMDYAEITVDPKFHR---HLIGKGGVNINRIKE-MHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLEL-ASRMENERTKDLIIEQRFHRAIIGQKGEKIKEVRDKFPEVIINFPDPAHKSDIVQLRGPRTEVEKCSKFMQKMVAEMVENSHSVSVPIFKQFHRNIIGKGGSNIKKIREETNTK--IDLPAENSNSEMIVITGKK 635 HSP 11 Score: 74.7146 bits (182), Expect = 7.188e-13 Identity = 62/280 (22.14%), Postives = 132/280 (47.14%), Query Frame = 2
Query: 2894 KLEITGHKDKITLAKEEIIKLF-----VLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNK---NSKFTKVIVTGSSES-------------------VVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLES--------GIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIV-----KVKGTKESVDRCADEL 3613
K+ + G K+ + AK+ + ++ + +E +P++F+ +++G KG +I+++ H V IK P + + ++ + E+ V++ +KE E +E +K + ++ + VP + HR +IG KG I K+ + V I VP D ++I+I G ++++AK +L V +L++ + +D + H ++IG +GA + ++ ++ V I FP + E ++ + K ++++ DEL
Sbjct: 848 KVSLKGAKECVEAAKKRMQEMIEDLDAQVTMECVIPQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDPQAAHSEAPAQENGEANGELKESVDPEAPKRCDVIVISGRKERCEAA--IEALKA---LVPVTIEVDVPFELHRYIIGQKGSGIRKMMDEFEVNIQVPGPDLQSDKISITGLASHLDRAKEGLLERVKELQADQEDRALRSFKLTITVDPKYHPKIIGRKGAIITNIRTEHDVNIQFPEKNDENQDQITITGYEHKAIAARDAIQAIVDEL 1122 HSP 12 Score: 53.5286 bits (127), Expect = 1.898e-6 Identity = 37/119 (31.09%), Postives = 59/119 (49.58%), Query Frame = 3
Query: 102 FITDTF-VIPYEERRYIDSESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQ---TQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDP 446
+ DT V P R ++ L+ L E GV V T QS V + G K+ V AKK + +++ Q + IP++FHRS++G KGS +++ + + Q+ FP+ DP
Sbjct: 802 IVEDTMNVDPKHHRYFVARRGQVLRDLADEYG---GVMVSFPRTASQSDKVSLKGAKECVEAAKKRMQEMIEDLDAQVTMECVIPQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDP 917
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Medaka
Match: hdlbpb (vigilin [Source:NCBI gene;Acc:101163851]) HSP 1 Score: 329.717 bits (844), Expect = 2.077e-114 Identity = 217/704 (30.82%), Postives = 384/704 (54.55%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVS 2653
K +H N + + + R+ IPP S+ K+EI I+G+++ VA A + + Y K + +E+ K +HKYIIGP+G+ ++EIL+ T V V++ + S TI L G D LGPALT V+ K++SV ++ + AP WLH+FIIGK G N+ ++ P + + F D +I +EG EEV + +++ + DL R+ E+ ID +H+IG G NI +I+++ S+ P+D + ++ I G P GV+ + L++ ++ +EN + KD+I +IGQ+G+ +KE++D+FPEV INFP P SD++ L GP+ +V++ A LQK A L+E+++S V I ++HK IIG+ G I+++ + TNTK +P + ++ IVITGK+ + E A R+ I + +N+ E + I LH LIG G + S+ E CGG V I FPS + S+ V I+G +V KA+ +L L EE +N+ Q E ++FL ++++R+ RI + ++ + I+G++E V L+ + +V +++ + + +F + ++ R + + V ++ ++ + + +G +++AK I II+D E+ ++ L + +R + + ++ I+++++VQI+ P + + N VS
Sbjct: 221 KAFHPFIAGAHNRLVQELSQETGARISIPPPSLPKDEIVITGEKEAVALAVNRIRAIYEDKKRKTTTISVEVKKSQHKYIIGPKGNTLQEILEATGVSVEMPPL---DSSSETIILRGEPDKLGPALTQVYAKAKSVMVVEVSAPAWLHRFIIGKKGQNIGRITQQLPRVHIEFTDGEERISLEGPTEEVEQAQIQIQEIIKDLLVRMDYSEIIIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERSNLIRIEGDPKGVQLARRELIEMVQRMENERTKDLIVEQKFHRTIIGQKGEKIKEVRDKFPEVIINFPDPSQKSDIVQLRGPKNEVEKCAKFLQKIIAELIENSYSLSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEMIVITGKKVNCEVARDRILAIQRELANLKETEVTIPAKLHNSLIGSKGCLVRSIMEDCGG-VHIHFPSEGSGSDKVTIRGPVGEVEKAKKQLLQLAEEKQVNNFTAELQAKPEYHKFLIGRGGANIRRVRDRTGARIIFPSPDDTEQELITIVGKEEAVRQAQRELETLVKNLDDVVEDSMEVDVRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGASSQK---VTLKGAKDCVEAAKKRIQEIIEDLESQVSVELAIPQRYHRAVMGPKGCRIQHITREHEVQIKFPERDDSAEALVQENGDVS 917 HSP 2 Score: 105.145 bits (261), Expect = 2.077e-114 Identity = 55/137 (40.15%), Postives = 82/137 (59.85%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
IT F +P EERRY D+ + K+ +I G +E ++ DQ L + ++G+ D V +A+K I +QTQ ++ IPKE HR +IG G +ELE T+T+++ P+ DPST I+I G+K+GIE AR I
Sbjct: 66 ITQVFHVPLEERRYKDNSQFGEGEEAKVCLDIMQRTGAHIELSLAKDQGLSIMVTGKLDSVMKARKEIVARLQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIAIPRPEDPSTSIRITGTKEGIEKARHEI 202 HSP 3 Score: 124.405 bits (311), Expect = 3.687e-28 Identity = 76/242 (31.40%), Postives = 144/242 (59.50%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVD 3619
+ I+G +K LAK ++ L + +V V E + +IG KG I+K+ ++V+I +P + + VTG + +V AK+ ++E V++L+ +++R +R+F + + V ++H K+IG KG IS+IR + V I P + D + I I G++ NVE+A+ + V +L+ + + +++D R H+R+IG+RG + +L +++KV+I FP G+ ++ + V V G E++ D L++
Sbjct: 930 ITISGRAEKCDLAKAALLALVPITEDVEVSYELHRYIIGQKGSGIRKMMEEYEVNIWVPQPEKQLDVIKVTGLAANVERAKQGLLERVKDLQAEQEDRALRSFKVTMSVDPKFHPKIIGRKGAVISQIRKDHDVSIQFPDKGDEQQDLIVISGYERNVEEARQAIQQLVAELQEMVSQDIHLDPRTHARIIGARGKAIRKLMEEFKVDIRFPQPGS--DQPDRVTVTGLPETISSAVDHLLN 1169 HSP 4 Score: 116.701 bits (291), Expect = 9.163e-26 Identity = 153/709 (21.58%), Postives = 299/709 (42.17%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAE-DKSHLRII--GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNI--------KSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSST-ISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFV---LDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFV-NKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLE 3667
+++ S V IP ++H+ +IG+ G ++EL T TK IP + + I ITG ++ +E A + I ++ + + K H + G + L + G R++I P P + ++I G K+ V A ++ + E+ ++ ++++ L+++ E V + D S II G+ + + L +V KA+S M +V + ++ +K + RI + + ++++ + + GP + + + KD ++ + DY +I+D+R +++ + ++ I ++Y+V ++IP E N I + + + V N D++ E + G K I E+ + I+ K I ++ P+ ++E K ++II + L V + ++F+ +IG G IKK+R I +P +NS +++TG + A+ I+ EL +K+ + +PA+ H LIG+KG + I V I PS+ S +++TI+G VEKAK ++L K + L H LIG GAN+ +++ + I+FP + E ++ + G +E+V + EL + D + D M+++
Sbjct: 137 LQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIAIPRPEDPSTSIRITGTKEGIEKARHEILLISAEQDKRAVERLSLEKAFHPFIAGAHNRLVQELSQETGARISI--PPPSLPKDEIVITGEKEAVALAVNRIRAIYEDKKRKTTTISVEVKKSQHKYIIGPKGNTLQEILEATGVSVEMPPLDSSSETIILRGEPDKLGPALTQVYAKAKSVM--VVEVSAPAWLHRFIIGKKGQNIGRITQ-QLPRVHIEFTDGEER------ISLEGPTEEVEQAQIQIQEIIKDLLVRM--DY-----SEIIIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERSNLIRIEGDPKGVQLARRELIEMVQRME-------------NERTKDLIVEQKFHRTIIGQKGEKIKEVRDKFPEVIINFPDPSQKSDIVQLRGPKNEVE------KCAKFLQKIIAELIENSYSLSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEMIVITGKKVNCEVARDRILAIQRELANLKE--------TEVTIPAKLHNSLIGSKGCLVRSIMEDCGGVHIHFPSEGSGSDKVTIRGPVGEVEKAKKQLLQLAEEKQVNNFTAELQAKPEYHKFLIGRGGANIRRVRDRTGARIIFP--SPDDTEQELITIVGKEEAVRQAQRELETLVKNLDDVVEDSMEVD 798 HSP 5 Score: 112.079 bits (279), Expect = 2.233e-24 Identity = 183/874 (20.94%), Postives = 379/874 (43.36%), Query Frame = 2
Query: 1013 IIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDV----DQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETY--SVRISEAEDKSHL-RIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK--------SNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDY-ENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNH---------LPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVI-VTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGE 3550
I+ ++G++ ++LS+ K D+ I++ G + V++ K+ V LQ++ S + I + +IG G +Q++ +T I PR + + I GT G+E + +L + + + P + G + ++E+ RI+ PPP D I + G ++ V +++ AI + K + V++ HK IIG +G T++E+ +AT ++P ++ I++ G+ + A+ +V Y K +++ + + LH +IGK G I + + RV I F + + ++G ++V +AQ+++ ++++++ + + L + +++E Y SVRI + ++S+L RI G + V Q AR E+ +V DL QK E + RD ++ + + I++ RGP ++ + II + EN+ + ++ + ++ KNI + +I ++ +I +P I+ V+ + + + + + + P H L IM + G H SE + TI + P+ ++ + +L + LA+E+ + F +L+ E++ LIG G I+++R I P+ + ++I + G E+V A++E+ V+ L+ + ++ ++ V ++HR + +G+ + ++ + V +S P ++ ++T++G KD VE AK + + LES + L I R H ++G +G + + ++++V+I FP R E
Sbjct: 97 IMQRTGAH-IELSLAK--------DQGLSIMVTGKLDSVMKAR---KEIVARLQTQASAT-VAIPKEHHRFVIGKNGEKLQELELKTATKIAIPRPEDPSTSIRITGTKEGIEKARHEILLISAEQDKRAVERLSLEKAFHPFIAGAHNRLVQELSQETG-ARISIPPPSLPKDEIVITGEKEAVALAVNRIRAIYEDKK----RKTTTISVEVKKSQHKYIIGPKGNTLQEILEATGVSVEMPPLDSSSETIILRGEPDKLGPALTQV---YAKAKSVMVVEVSAPAWLHRFIIGKKGQNIGRITQQLP-RVHIEFTDGE---ERISLEGPTEEVEQAQIQIQEIIKDLLVRMDYSEIIIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERSNLIRIEGDPKGV-------QLARRELIEMVQRMENERTKDLIVEQKFHRTIIGQKGEKIKEVRDKFPEVIINFPDPSQ---KSDIVQLRGPKNEVEKCAKFLQKIIAELIENSYSLSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEMIVITGKKVNCEVARDRIL-AIQRELANLKETEVTIPAKLHNSLIGSKGCLVRSIM--EDCGGVHIHFPSEGSGSDKVTI-------RGPVGEVEKAKKQL--------LQLAEEKQVNNFTAELQAK--PEYHKFLIGRGGANIRRVRDRTGARIIFPSPDDTEQELITIVGKEEAVRQAQRELETLVKNLDDVVED--------SMEVDVRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGASSQKVTLKGAKDCVEAAKKRIQEIIEDLESQVSVELAIPQRYHRAVMGPKGCRIQHITREHEVQIKFPERDDSAE 907 HSP 6 Score: 105.916 bits (263), Expect = 2.042e-22 Identity = 122/530 (23.02%), Postives = 242/530 (45.66%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLS------VNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINF-PRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHA-FLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENV 2215
I ++G D V +A I+ + Q AT + IPKE H+++IG G ++E+ +T+ + I E +I + G K+ + A + L+ + + + + H FI G + +LS ++ P L ++IVI G E V ++ D + + + + +E+ K+IIG G +Q+I + T S+ P D + + + + G P + + +++ + + H +IG++G+ + I + P V I F D + + ISL GP ++V+Q +Q+ LV ++S ++ I ++H+ +IG+ G I + + +IP SE ++ I I G K V+ A + + + ++ N + + + H +IG+ G +I +++ V I FP P S+ V ++G K++V K L ++ E+ ++Y + + ++ + +KK+RE + +I +E + + I G+K N
Sbjct: 117 IMVTGKLDSVMKARKEIVARLQTQASAT-----VAIPKEHHRFVIGKNGEKLQELELKTATKIAIPR---PEDPSTSIRITGTKEGIEKARHEILLISAEQDKRAVERLSLEKAFHPFIAGAHNRLVQELSQETGARISIPPPSL----PKDEIVITGEKEAVALAVNRIRAIYEDKKRKTTTISVEVKKSQHKYIIGPKGNTLQEILEATGVSVEMPPLDSSSETIILRGEPDKLGPALTQVYAKAKSVMVVEVSAPAWLHRF---IIGKKGQNIGRITQQLPRVHIEF--TDGE-ERISLEGPTEEVEQAQIQIQEIIKDLLVRMDYS-EIIIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERSNLIRIEGDPKGVQLARRELIEMVQRMENERTKDLIVEQKFHRTIIGQKGEKIKEVRDKF-PEVIINFPDPSQKSDIVQLRGPKNEVEKCAKFLQKIIAELIENSYSLSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEMIVITGKKVNC 626 HSP 7 Score: 84.3445 bits (207), Expect = 2.643e-21 Identity = 122/570 (21.40%), Postives = 258/570 (45.26%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNI----IAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRF----EDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKE-TNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKK-----------------VKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDA----IKRVCNIYXXXXXXXXXXXXXXXXL----HTILIGKGGNRINSLKESCGGRVTIRFPSP-DADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI 2161
K++H+ K + + E + ++D+P + + I I+G + A +L ++ A +E ++ IP + H +IG +G ++ I++ V I+ GS + + + GP + A + +E + + A HKF+IG+ G+N+ + ++ ++ F + E I I G E V + L+ V +L + + ME+D +H + G ++++ +E +++FPR + + VT+ G VEA K + + IE +E+ ++ ++G +G ++ I R EV+I FP D ++ +L+ DV + +K + S ++K V++ ++ H+ IIG++G IR++ + +P ++ D I +TG +VE A ++RV ++ ++ + +++ ++ H +IG+ G I+ +++ V+I+FP D + ++I G + +V +A+ + LV E+ Q + + + + ++KL E + V I
Sbjct: 580 KQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEMIVITGKKVNCEVARDRILAIQREL-ANLKETEVTIPAKLHNSLIGSKGCLVRSIMEDCG-GVHIHFPSEGSGS-DKVTIRGPVGEVEKAKKQLLQLAEEKQVNNFTAELQAKPEYHKFLIGRGGANIRRVR-DRTGARIIFPSPDDTEQELITIVGKEEAVRQAQRELETLVKNLDD-VVEDSMEVDVRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGASSQKVTLKGAKDCVEAAKKRIQEIIEDLESQVSVELAIPQRYHRAVMGPKGCRIQHI-TREHEVQIKFPERDDSAE--ALVQENGDVSLETEFVPRKCDIITISGRAEKCDLAKAALLALVPITEDVEVSYELHRYIIGQKGSGIRKMMEEYEVNIWVPQPEKQLDVIKVTGLAANVERAKQGLLERVKDLQAEQEDRALRSFKVTMSVDPKFHPKIIGRKGAVISQIRKDHD--VSIQFPDKGDEQQDLIVISGYERNVEEARQAIQQLVAELQ-EMVSQDIHLDPRTHARIIGARGKAIRKLMEEFKVDI 1138 HSP 8 Score: 39.2762 bits (90), Expect = 2.643e-21 Identity = 25/97 (25.77%), Postives = 51/97 (52.58%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITRLVQT---QFEISLDIPKEFHRSLIGNKGSLHKELENN-TSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEF 527
+ I G V A++ + +VQ + L + ++FHR++IG KG KE+ + ++FP + S +++++G K+ +E + +QK + E
Sbjct: 471 IRIEGDPKGVQLARRELIEMVQRMENERTKDLIVEQKFHRTIIGQKGEKIKEVRDKFPEVIINFPDPSQKSDIVQLRGPKNEVEKCAKFLQKIIAEL 567 HSP 9 Score: 102.064 bits (253), Expect = 3.137e-21 Identity = 101/402 (25.12%), Postives = 184/402 (45.77%), Query Frame = 2
Query: 2408 ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN--GSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINE-ITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVD 3595
++ G + ++ A+ E+I ++ EN T++LIV+++ + I ++ E+ K+ +V I +P+P I EK + ++ L N L I + + N G NI +I E ++ I + + I + E+ +D+I + E+ L + EV +P + + +LIG+KG ++ + V I P++ S KV + G V AKK++++ EE +++ NF+ L +YH+ LIG G I ++R + RI PS D E ITI G ++ V +A+ E+ V L+ + + + +D R H + RG L +L ++Y V + FP G ++ V +KG K+ V+
Sbjct: 470 LIRIEGDPKGVQLARRELIEMVQRMENERTKDLIVEQKFHRTIIGQKGEKIKEVRDKFPEVIIN---FPDPSQKSDIVQLRGPKNEVEK-------CAKFLQKIIAELIENSYSLSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEMIVITGKKVNCEVA--RDRILAIQRELANL--KETEVTIPAKLHNSLIGSKGCLVRSIMEDCGGVHIHFPSEGSGSDKVTIRGPVGEVEKAKKQLLQLAEE-------KQVNNFTAELQAKPEYHKFLIGRGGANIRRVRDRTGARIIFPSPDDTEQELITIVGKEEAVRQAQRELETLVKNLDDVVEDSMEVDVRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGASSQK---VTLKGAKDCVE 847 HSP 10 Score: 72.7886 bits (177), Expect = 1.668e-17 Identity = 100/462 (21.65%), Postives = 202/462 (43.72%), Query Frame = 2
Query: 533 NWTTSGRN*KRYHQLACHKSNTALHTVLERKSLRLDIP-PFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRF---EDEANQIVIEGSPEEVIEVSEYLKKK--VFDLQSRLSQL---------------EMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTK-KVVTIIGTPHGVEAVKAILLXXXX--XXXXXXXXXXXFTHTL------MPQLIGQQGKGLKEIKDRFPEVRINFPPP-DADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIP-AASEENDEIVITGKQKDVEDAIKRVCNI 1813
N+T + YH+ + + V +R R+ P P ++ I+I G + V QA L + ++ +E+ H++ + RG ++E+ ++ V ++ R S + L G KD + A + + ES + + P H+ ++G G + ++ + +Q++F +D A +V E +V +E++ +K + + R + ++E+ + ++IIG G I+K+ +E +I P+ + V+ + G VE K LL+ ++ E + F T+ P++IG++G + +I+ +V I FP D DLI + G ++V++ +Q+ A L E S+ + + + H IIG G IR+L + + P S++ D + +TG + + A+ + N+
Sbjct: 717 NFTAELQAKPEYHKFLIGRGGANIRRVRDRTGARIIFPSPDDTEQELITIVGKEEAVRQAQRELETLVKNLDDVVED-SMEVDVRHHRHFVCRRGQVLRELAEEYG-GVAVSFPRTG-ASSQKVTLKGAKDCVEAAKKRIQEIIEDLESQVSVELAIPQRYHRAVMGPKGCRIQHIT-REHEVQIKFPERDDSAEALVQENG--DVSLETEFVPRKCDIITISGRAEKCDLAKAALLALVPITEDVEVSYELHRYIIGQKGSGIRKMMEEYEVNIWVPQPEKQLDVIKVTGLAANVERAKQGLLERVKDLQAEQEDRALRSFKVTMSVDPKFHPKIIGRKGAVISQIRKDH-DVSIQFPDKGDEQQDLIVISGYERNVEEARQAIQQLVAELQEM-VSQDIHLDPRTHARIIGARGKAIRKLMEEFKVDIRFPQPGSDQPDRVTVTGLPETISSAVDHLLNL 1170 HSP 11 Score: 37.7354 bits (86), Expect = 1.668e-17 Identity = 37/124 (29.84%), Postives = 58/124 (46.77%), Query Frame = 3
Query: 207 VRVETNVTPD------QSLGVFISGQKDKVCEAKKYITRLVQTQF----EISLDIPKEFHRSLIGNKGSLHKE-LENNTSTQVSFPKINDPSTLIKIKGSKDGIEHA-RQLIQ----KNVDEFS 530
+R ETN D S + I+G+K CE + +Q + E + IP + H SLIG+KG L + +E+ + FP S + I+G +E A +QL+Q K V+ F+
Sbjct: 597 IREETNTKIDLPTENSNSEMIVITGKKVN-CEVARDRILAIQRELANLKETEVTIPAKLHNSLIGSKGCLVRSIMEDCGGVHIHFPSEGSGSDKVTIRGPVGEVEKAKKQLLQLAEEKQVNNFT 719 HSP 12 Score: 65.0846 bits (157), Expect = 5.469e-10 Identity = 42/165 (25.45%), Postives = 83/165 (50.30%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESV 3592
++VTG +SV+ A+KEIV ++ S + +P ++HR +IG GE + ++ K + +I++P + I I G K+ +EKA++E+L + + +E L+++ H + G+ + +L ++ I P +E + + G KE+V
Sbjct: 117 IMVTGKLDSVMKARKEIVARLQT-----------QASATVAIPKEHHRFVIGKNGEKLQELELKTATKIAIPRPEDPSTSIRITGTKEGIEKARHEILLISAEQDKRAVERLSLEKAFHPFIAGAHNRLVQELSQETGARISIPPPSLPKDE---IVITGEKEAV 267
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Medaka
Match: hdlbpb (vigilin [Source:NCBI gene;Acc:101163851]) HSP 1 Score: 329.331 bits (843), Expect = 2.372e-114 Identity = 214/687 (31.15%), Postives = 379/687 (55.17%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYF-QKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIP 2602
K +H N + + + R+ IPP S+ K+EI I+G+++ VA A + + Y K + +E+ K +HKYIIGP+G+ ++EIL+ T V V++ + S TI L G D LGPALT V+ K++SV ++ + AP WLH+FIIGK G N+ ++ P + + F D +I +EG EEV + +++ + DL R+ E+ ID +H+IG G NI +I+++ S+ P+D + ++ I G P GV+ + L++ ++ +EN + KD+I +IGQ+G+ +KE++D+FPEV INFP P SD++ L GP+ +V++ A LQK A L+E+++S V I ++HK IIG+ G I+++ + TNTK +P + ++ IVITGK+ + E A R+ I + +N+ E + I LH LIG G + S+ E CGG V I FPS + S+ V I+G +V KA+ +L L EE +N+ Q E ++FL ++++R+ RI + ++ + I+G++E V L+ + +V +++ + + +F + ++ R + + V ++ ++ + + +G +++AK I II+D E+ ++ L + +R + + ++ I+++++VQI+ P
Sbjct: 229 KAFHPFIAGAHNRLVQELSQETGARISIPPPSLPKDEIVITGEKEAVALAVNRIRAIYEDKKRKTTTISVEVKKSQHKYIIGPKGNTLQEILEATGVSVEMPPL---DSSSETIILRGEPDKLGPALTQVYAKAKSVMVVEVSAPAWLHRFIIGKKGQNIGRITQQLPRVHIEFTDGEERISLEGPTEEVEQAQIQIQEIIKDLLVRMDYSEIIIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERSNLIRIEGDPKGVQLARRELIEMVQRMENERTKDLIVEQKFHRTIIGQKGEKIKEVRDKFPEVIINFPDPSQKSDIVQLRGPKNEVEKCAKFLQKIIAELIENSYSLSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEMIVITGKKVNCEVARDRILAIQRELANLKETEVTIPAKLHNSLIGSKGCLVRSIMEDCGG-VHIHFPSEGSGSDKVTIRGPVGEVEKAKKQLLQLAEEKQVNNFTAELQAKPEYHKFLIGRGGANIRRVRDRTGARIIFPSPDDTEQELITIVGKEEAVRQAQRELETLVKNLDDVVEDSMEVDVRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGASSQK---VTLKGAKDCVEAAKKRIQEIIEDLESQVSVELAIPQRYHRAVMGPKGCRIQHITREHEVQIKFP 908 HSP 2 Score: 105.531 bits (262), Expect = 2.372e-114 Identity = 55/137 (40.15%), Postives = 82/137 (59.85%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSE---SNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLI 506
IT F +P EERRY D+ + K+ +I G +E ++ DQ L + ++G+ D V +A+K I +QTQ ++ IPKE HR +IG G +ELE T+T+++ P+ DPST I+I G+K+GIE AR I
Sbjct: 74 ITQVFHVPLEERRYKDNSQFGEGEEAKVCLDIMQRTGAHIELSLAKDQGLSIMVTGKLDSVMKARKEIVARLQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIAIPRPEDPSTSIRITGTKEGIEKARHEI 210 HSP 3 Score: 142.51 bits (358), Expect = 1.075e-33 Identity = 98/330 (29.70%), Postives = 175/330 (53.03%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQ-DSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSW 3883
+ I+G +K LAK ++ L + +V V E + +IG KG I+K+ ++V+I +P + + VTG + +V AK+ ++E V++L+ +++R +R+F + + V ++H K+IG KG IS+IR + V I P + D + I I G++ NVE+A+ + V +L+ + + +++D R H+R+IG+RG + +L +++KV+I FP G+ ++ + V V G E++ D L++ + + + L S + S GF +R+APW+A GN SSA D G + P QTS+W
Sbjct: 941 ITISGRAEKCDLAKAALLALVPITEDVEVSYELHRYIIGQKGSGIRKMMEEYEVNIWVPQPEKQLDVIKVTGLAANVERAKQGLLERVKDLQAEQEDRALRSFKVTMSVDPKFHPKIIGRKGAVISQIRKDHDVSIQFPDKGDEQQDLIVISGYERNVEEARQAIQQLVAELQEMVSQDIHLDPRTHARIIGARGKAIRKLMEEFKVDIRFPQPGS--DQPDRVTVTGLPETISSAVDHLLNLEEEYMLSVTETETLAAYMKPP--SRYGGGGGIGMDDSSGGPAKGFVVRDAPWNAAGNKAPDVSSAEDFPTF---GTSV-APKQTSAW 1262 HSP 4 Score: 117.087 bits (292), Expect = 7.360e-26 Identity = 153/709 (21.58%), Postives = 299/709 (42.17%), Query Frame = 2
Query: 1607 VESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAE-DKSHLRII--GQKENVLNTL-EVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNI--------KSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN----GSKYHNISEINSEVSST-ISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFV---LDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFV-NKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLE 3667
+++ S V IP ++H+ +IG+ G ++EL T TK IP + + I ITG ++ +E A + I ++ + + K H + G + L + G R++I P P + ++I G K+ V A ++ + E+ ++ ++++ L+++ E V + D S II G+ + + L +V KA+S M +V + ++ +K + RI + + ++++ + + GP + + + KD ++ + DY +I+D+R +++ + ++ I ++Y+V ++IP E N I + + + V N D++ E + G K I E+ + I+ K I ++ P+ ++E K ++II + L V + ++F+ +IG G IKK+R I +P +NS +++TG + A+ I+ EL +K+ + +PA+ H LIG+KG + I V I PS+ S +++TI+G VEKAK ++L K + L H LIG GAN+ +++ + I+FP + E ++ + G +E+V + EL + D + D M+++
Sbjct: 145 LQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIAIPRPEDPSTSIRITGTKEGIEKARHEILLISAEQDKRAVERLSLEKAFHPFIAGAHNRLVQELSQETGARISI--PPPSLPKDEIVITGEKEAVALAVNRIRAIYEDKKRKTTTISVEVKKSQHKYIIGPKGNTLQEILEATGVSVEMPPLDSSSETIILRGEPDKLGPALTQVYAKAKSVM--VVEVSAPAWLHRFIIGKKGQNIGRITQ-QLPRVHIEFTDGEER------ISLEGPTEEVEQAQIQIQEIIKDLLVRM--DY-----SEIIIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERSNLIRIEGDPKGVQLARRELIEMVQRME-------------NERTKDLIVEQKFHRTIIGQKGEKIKEVRDKFPEVIINFPDPSQKSDIVQLRGPKNEVE------KCAKFLQKIIAELIENSYSLSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEMIVITGKKVNCEVARDRILAIQRELANLKE--------TEVTIPAKLHNSLIGSKGCLVRSIMEDCGGVHIHFPSEGSGSDKVTIRGPVGEVEKAKKQLLQLAEEKQVNNFTAELQAKPEYHKFLIGRGGANIRRVRDRTGARIIFP--SPDDTEQELITIVGKEEAVRQAQRELETLVKNLDDVVEDSMEVD 806 HSP 5 Score: 112.849 bits (281), Expect = 1.590e-24 Identity = 188/880 (21.36%), Postives = 386/880 (43.86%), Query Frame = 2
Query: 1013 IIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDV----DQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETY--SVRISEAEDKSHL-RIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQK--------SNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDY-ENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSK--TISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNH---------LPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVI-VTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIR--GTEGEEA 3556
I+ ++G++ ++LS+ K D+ I++ G + V++ K+ V LQ++ S + I + +IG G +Q++ +T I PR + + I GT G+E + +L + + + P + G + ++E+ RI+ PPP D I + G ++ V +++ AI + K + V++ HK IIG +G T++E+ +AT ++P ++ I++ G+ + A+ +V Y K +++ + + LH +IGK G I + + RV I F + + ++G ++V +AQ+++ ++++++ + + L + +++E Y SVRI + ++S+L RI G + V Q AR E+ +V DL QK E + RD ++ + + I++ RGP ++ + II + EN+ + ++ + ++ KNI + +I ++ +I +P E NS+ I+ V+ + + + + + + P H L IM + G H SE + TI + P+ ++ + +L + LA+E+ + F +L+ E++ LIG G I+++R I P+ + ++I + G E+V A++E+ V+ L+ + ++ ++ V ++HR + +G+ + ++ + V +S P ++ ++T++G KD VE AK + + LES + L I R H ++G +G + + ++++V+I FP R G+EA
Sbjct: 105 IMQRTGAH-IELSLAK--------DQGLSIMVTGKLDSVMKAR---KEIVARLQTQASAT-VAIPKEHHRFVIGKNGEKLQELELKTATKIAIPRPEDPSTSIRITGTKEGIEKARHEILLISAEQDKRAVERLSLEKAFHPFIAGAHNRLVQELSQETG-ARISIPPPSLPKDEIVITGEKEAVALAVNRIRAIYEDKK----RKTTTISVEVKKSQHKYIIGPKGNTLQEILEATGVSVEMPPLDSSSETIILRGEPDKLGPALTQV---YAKAKSVMVVEVSAPAWLHRFIIGKKGQNIGRITQQLP-RVHIEFTDGE---ERISLEGPTEEVEQAQIQIQEIIKDLLVRMDYSEIIIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERSNLIRIEGDPKGV-------QLARRELIEMVQRMENERTKDLIVEQKFHRTIIGQKGEKIKEVRDKFPEVIINFPDPSQ---KSDIVQLRGPKNEVEKCAKFLQKIIAELIENSYSLSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPT--ENSNSEMIVITGKKVNCEVARDRIL-AIQRELANLKETEVTIPAKLHNSLIGSKGCLVRSIM--EDCGGVHIHFPSEGSGSDKVTI-------RGPVGEVEKAKKQL--------LQLAEEKQVNNFTAELQAK--PEYHKFLIGRGGANIRRVRDRTGARIIFPSPDDTEQELITIVGKEEAVRQAQRELETLVKNLDDVVED--------SMEVDVRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGASSQKVTLKGAKDCVEAAKKRIQEIIEDLESQVSVELAIPQRYHRAVMGPKGCRIQHITREHEVQIKFPERDDSAAGQEA 919 HSP 6 Score: 105.531 bits (262), Expect = 2.561e-22 Identity = 122/530 (23.02%), Postives = 242/530 (45.66%), Query Frame = 2
Query: 671 ISISGDRDGVAQA-ASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLS------VNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHA-FLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENV 2215
I ++G D V +A I+ + Q AT + IPKE H+++IG G ++E+ +T+ + I E +I + G K+ + A + L+ + + + + H FI G + +LS ++ P L ++IVI G E V ++ D + + + + +E+ K+IIG G +Q+I + T S+ P D + + + + G P + + +++ + + H +IG++G+ + I + P V I F D + + ISL GP ++V+Q +Q+ LV ++S ++ I ++H+ +IG+ G I + + +IP SE ++ I I G K V+ A + + + ++ N + + + H +IG+ G +I +++ V I FP P S+ V ++G K++V K L ++ E+ ++Y + + ++ + +KK+RE + +I +E + + I G+K N
Sbjct: 125 IMVTGKLDSVMKARKEIVARLQTQASAT-----VAIPKEHHRFVIGKNGEKLQELELKTATKIAIPR---PEDPSTSIRITGTKEGIEKARHEILLISAEQDKRAVERLSLEKAFHPFIAGAHNRLVQELSQETGARISIPPPSL----PKDEIVITGEKEAVALAVNRIRAIYEDKKRKTTTISVEVKKSQHKYIIGPKGNTLQEILEATGVSVEMPPLDSSSETIILRGEPDKLGPALTQVYAKAKSVMVVEVSAPAWLHRF---IIGKKGQNIGRITQQLPRVHIEF--TDGE-ERISLEGPTEEVEQAQIQIQEIIKDLLVRMDYS-EIIIDQRFHRHLIGKNGANINRIKEQYKVSVRIPQDSERSNLIRIEGDPKGVQLARRELIEMVQRMENERTKDLIVEQKFHRTIIGQKGEKIKEVRDKF-PEVIINFPDPSQKSDIVQLRGPKNEVEKCAKFLQKIIAELIENSYSLSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEMIVITGKKVNC 634 HSP 7 Score: 102.064 bits (253), Expect = 3.020e-21 Identity = 101/402 (25.12%), Postives = 184/402 (45.77%), Query Frame = 2
Query: 2408 ILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKY-QVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSN--GSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKK-LRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINE-ITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKY-KVEIVFPIRGTEGEEANIVKVKGTKESVD 3595
++ G + ++ A+ E+I ++ EN T++LIV+++ + I ++ E+ K+ +V I +P+P I EK + ++ L N L I + + N G NI +I E ++ I + + I + E+ +D+I + E+ L + EV +P + + +LIG+KG ++ + V I P++ S KV + G V AKK++++ EE +++ NF+ L +YH+ LIG G I ++R + RI PS D E ITI G ++ V +A+ E+ V L+ + + + +D R H + RG L +L ++Y V + FP G ++ V +KG K+ V+
Sbjct: 478 LIRIEGDPKGVQLARRELIEMVQRMENERTKDLIVEQKFHRTIIGQKGEKIKEVRDKFPEVIIN---FPDPSQKSDIVQLRGPKNEVEK-------CAKFLQKIIAELIENSYSLSVPIFKQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEMIVITGKKVNCEVA--RDRILAIQRELANL--KETEVTIPAKLHNSLIGSKGCLVRSIMEDCGGVHIHFPSEGSGSDKVTIRGPVGEVEKAKKQLLQLAEE-------KQVNNFTAELQAKPEYHKFLIGRGGANIRRVRDRTGARIIFPSPDDTEQELITIVGKEEAVRQAQRELETLVKNLDDVVEDSMEVDVRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGASSQK---VTLKGAKDCVE 855 HSP 8 Score: 83.9593 bits (206), Expect = 3.549e-21 Identity = 133/618 (21.52%), Postives = 275/618 (44.50%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNI----IAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRF----EDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKE-TNCSINFPR-DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPD-------------ADSDLISLIGPRK-DVDQVAAILQKKHAFLVESN----FSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDA----IKRVCNIYXXXXXXXXXXXXXXXXL----HTILIGKGGNRINSLKESCGGRVTIRFPSP-DADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKS----HLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALS 2290
K++H+ K + + E + ++D+P + + I I+G + A +L ++ A +E ++ IP + H +IG +G ++ I++ V I+ GS + + + GP + A + +E + + A HKF+IG+ G+N+ + ++ ++ F + E I I G E V + L+ V +L + + ME+D +H + G ++++ +E +++FPR + + VT+ G VEA K + + IE +E+ ++ ++G +G ++ I R EV+I FP D D L + PRK D+ ++ +K ++ V++ ++ H+ IIG++G IR++ + +P ++ D I +TG +VE A ++RV ++ ++ + +++ ++ H +IG+ G I+ +++ V+I+FP D + ++I G + +V +A+ + LV E+ Q + + + + ++KL E + V I + S + + G E + + ++ L E VTET L+
Sbjct: 588 KQFHKNIIGKGGANIKKIREETNTKIDLPTENSNSEMIVITGKKVNCEVARDRILAIQREL-ANLKETEVTIPAKLHNSLIGSKGCLVRSIMEDCG-GVHIHFPSEGSGS-DKVTIRGPVGEVEKAKKQLLQLAEEKQVNNFTAELQAKPEYHKFLIGRGGANIRRVR-DRTGARIIFPSPDDTEQELITIVGKEEAVRQAQRELETLVKNLDD-VVEDSMEVDVRHHRHFVCRRGQVLRELAEEYGGVAVSFPRTGASSQKVTLKGAKDCVEAAKKRIQEIIEDLESQVSVELAIPQRYHRAVMGPKGCRIQHIT-REHEVQIKFPERDDSAAGQEALVQENGDVSLETEFVPRKCDIITISGRAEKCDLAKAALLALVPITEDVEVSYELHRYIIGQKGSGIRKMMEEYEVNIWVPQPEKQLDVIKVTGLAANVERAKQGLLERVKDLQAEQEDRALRSFKVTMSVDPKFHPKIIGRKGAVISQIRKDHD--VSIQFPDKGDEQQDLIVISGYERNVEEARQAIQQLVAELQ-EMVSQDIHLDPRTHARIIGARGKAIRKLMEEFKVDIRFPQPGSDQPDRVTVTGLPETISSAVDHLLNLEEEYMLSVTETETLA 1196 HSP 9 Score: 39.2762 bits (90), Expect = 3.549e-21 Identity = 25/97 (25.77%), Postives = 51/97 (52.58%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITRLVQT---QFEISLDIPKEFHRSLIGNKGSLHKELENN-TSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEF 527
+ I G V A++ + +VQ + L + ++FHR++IG KG KE+ + ++FP + S +++++G K+ +E + +QK + E
Sbjct: 479 IRIEGDPKGVQLARRELIEMVQRMENERTKDLIVEQKFHRTIIGQKGEKIKEVRDKFPEVIINFPDPSQKSDIVQLRGPKNEVEKCAKFLQKIIAEL 575 HSP 10 Score: 69.3218 bits (168), Expect = 1.880e-16 Identity = 98/463 (21.17%), Postives = 200/463 (43.20%), Query Frame = 2
Query: 533 NWTTSGRN*KRYHQLACHKSNTALHTVLERKSLRLDIP-PFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRF----EDEANQIVIEGSPEEVIEVSEYLKKK--VFDLQSRLSQL---------------EMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTK-KVVTIIGTPHGVEAVKAILLXXXX--XXXXXXXXXXXFTHTLM------PQLIGQQGKGLKEIKDRFPEVRINFPPP-DADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIP-AASEENDEIVITGKQKDVEDAIKRVCNI 1813
N+T + YH+ + + V +R R+ P P ++ I+I G + V QA L + ++ +E+ H++ + RG ++E+ ++ V ++ R S + L G KD + A + + ES + + P H+ ++G G + ++ + +Q++F + A Q + +V +E++ +K + + R + ++E+ + ++IIG G I+K+ +E +I P+ + V+ + G VE K LL+ ++ E + F T+ P++IG++G + +I+ +V I FP D DLI + G ++V++ +Q+ A L E S+ + + + H IIG G IR+L + + P S++ D + +TG + + A+ + N+
Sbjct: 725 NFTAELQAKPEYHKFLIGRGGANIRRVRDRTGARIIFPSPDDTEQELITIVGKEEAVRQAQRELETLVKNLDDVVED-SMEVDVRHHRHFVCRRGQVLRELAEEYG-GVAVSFPRTG-ASSQKVTLKGAKDCVEAAKKRIQEIIEDLESQVSVELAIPQRYHRAVMGPKGCRIQHIT-REHEVQIKFPERDDSAAGQEALVQENGDVSLETEFVPRKCDIITISGRAEKCDLAKAALLALVPITEDVEVSYELHRYIIGQKGSGIRKMMEEYEVNIWVPQPEKQLDVIKVTGLAANVERAKQGLLERVKDLQAEQEDRALRSFKVTMSVDPKFHPKIIGRKGAVISQIRKDH-DVSIQFPDKGDEQQDLIVISGYERNVEEARQAIQQLVAELQEM-VSQDIHLDPRTHARIIGARGKAIRKLMEEFKVDIRFPQPGSDQPDRVTVTGLPETISSAVDHLLNL 1181 HSP 11 Score: 37.7354 bits (86), Expect = 1.880e-16 Identity = 37/124 (29.84%), Postives = 58/124 (46.77%), Query Frame = 3
Query: 207 VRVETNVTPD------QSLGVFISGQKDKVCEAKKYITRLVQTQF----EISLDIPKEFHRSLIGNKGSLHKE-LENNTSTQVSFPKINDPSTLIKIKGSKDGIEHA-RQLIQ----KNVDEFS 530
+R ETN D S + I+G+K CE + +Q + E + IP + H SLIG+KG L + +E+ + FP S + I+G +E A +QL+Q K V+ F+
Sbjct: 605 IREETNTKIDLPTENSNSEMIVITGKKVN-CEVARDRILAIQRELANLKETEVTIPAKLHNSLIGSKGCLVRSIMEDCGGVHIHFPSEGSGSDKVTIRGPVGEVEKAKKQLLQLAEEKQVNNFT 727 HSP 12 Score: 65.4698 bits (158), Expect = 4.787e-10 Identity = 42/165 (25.45%), Postives = 83/165 (50.30%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESV 3592
++VTG +SV+ A+KEIV ++ S + +P ++HR +IG GE + ++ K + +I++P + I I G K+ +EKA++E+L + + +E L+++ H + G+ + +L ++ I P +E + + G KE+V
Sbjct: 125 IMVTGKLDSVMKARKEIVARLQT-----------QASATVAIPKEHHRFVIGKNGEKLQELELKTATKIAIPRPEDPSTSIRITGTKEGIEKARHEILLISAEQDKRAVERLSLEKAFHPFIAGAHNRLVQELSQETGARISIPPPSLPKDE---IVITGEKEAV 275
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Medaka
Match: ENSORLT00000028704.1 (mex-3 RNA binding family member A [Source:NCBI gene;Acc:111948930]) HSP 1 Score: 57.7658 bits (138), Expect = 6.369e-8 Identity = 42/154 (27.27%), Postives = 70/154 (45.45%), Query Frame = 2
Query: 3005 LIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLA--------------LHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSN-INEITIQGHKDNVEKAKNEVLAFVNKLESGI 3421
++G +G KIK LR IK P + + ++TG E V A++EI+ E ++ R S + + VP + ++G KG TI +I+ + I PS+D + + EIT G N E+A+ E+ A + G+
Sbjct: 187 IVGRQGCKIKALRAKTNTYIKTPVRGEE-PVFLITGRKEDVALARREIISAAEHFSMLRASRNKLGVSFSGSPPTPLPGQTTIQVRVPYRVVGLVVGPKGSTIKRIQQQTCTYIVTPSRDRDPVFEIT--GSPSNAERAREEIEAHIAFRTGGL 337
BLAST of Vigilin High density lipoprotein-binding protein vs. Planmine SMEST
Match: SMESG000006601.1 (SMESG000006601.1) HSP 1 Score: 2150.17 bits (5570), Expect = 0.000e+0 Identity = 1116/1118 (99.82%), Postives = 1116/1118 (99.82%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSWRQIFPKSTIN 3913
KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKVVTIIGTPHGVEAVKAILLKTIETIENTKKKDIIFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYEKESNIIEIGIEISKNLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVPNKKSKSNPNEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTI SHVSAKPGEKPYMNGVDSSFSEEESFKSLSPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSWRQIFPKSTIN
Sbjct: 222 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKVVTIIGTPHGVEAVKAILLKTIETIENTKKKDIIFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYEKESNIIEIGIEISKNLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVPNKKSKSNPNEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTICYSHVSAKPGEKPYMNGVDSSFSEEESFKSLSPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSWRQIFPKSTIN 1339 HSP 2 Score: 380.948 bits (977), Expect = 1.026e-110 Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 3
Query: 3 DNNYNNNNKHIQFSNDITELSLIHSQNPSKLVQFITDTFVIPYEERRYIDSESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIE 560
DNNYNNNNKHIQFSNDITELSLIHSQNPSKLVQFITDTFVIPYEERRYIDSESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIE
Sbjct: 36 DNNYNNNNKHIQFSNDITELSLIHSQNPSKLVQFITDTFVIPYEERRYIDSESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIE 221 HSP 3 Score: 88.5817 bits (218), Expect = 2.919e-17 Identity = 121/532 (22.74%), Postives = 249/532 (46.80%), Query Frame = 2
Query: 671 ISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIA---IDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLR---FEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETN--CSINFPR--DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSK-KVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTI---LIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENV 2215
+ ISG +D V +A + ++ T E+ ++IPKE H+ +IG +GS KE+ + TS V ++N+ S I + G KD + A L+ + + I ++ H+ KS + L + + + +++L F + N+I I G + V + + L + +++ +L++EI K+IIG G I++I +T+ IN R + + + ++G + ++ E++ + H +IG+ G L ++ P +++ F + +++ I + G ++V +V+ L+KK F ++S S+ +++I K IIG G+ I+++ K TN P + + I G VE A+K + + + I + HT+ LIG+ G + +K+ V I FP PDADS+ + + G + DV + L + SN+ + ++ + ++ + + +++L + + + + +E+ + I G++++V
Sbjct: 118 VFISGQKDKVCEAKKYI----TRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSF--PKINDPS-TLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIEKRYHQLACHKSNTALHTV-LERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKF---IIGKSGSNLVDLSVNKPNIQLRF---EDEANQIVIEGSPEEVIEVSEYLKKK-VFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVE-AVKAI--LLKTIETIENTKKKDIIFTHTLMPQLIGQQGKGLKEIKDRF-PEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDV 629 HSP 4 Score: 77.7962 bits (190), Expect = 6.179e-14 Identity = 51/201 (25.37%), Postives = 102/201 (50.75%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEV--NVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQL 3496
I+G KDK+ AK+ I +L E+ ++P+EF+ +LIG KG K+L + P N T + + GS + + A++ I + V+E ++ ++ + + +YH+ + + + S+R+ +P + NEI+I G +D V +A + +L N++++ E+ + I H +IG RG+ + ++
Sbjct: 120 ISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQ--------VVEIEKRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEI 312 HSP 5 Score: 59.3066 bits (142), Expect = 2.217e-8 Identity = 43/179 (24.02%), Postives = 78/179 (43.58%), Query Frame = 2
Query: 3098 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIM 3634
V ++G + V AKK I V+ F ++L +P ++HR LIGNKG ++ S ++S P + I I+G KD +E A+ + V++ +V+ I+ R H L + ++ + + P + E + + G ++ V + A L+ N M
Sbjct: 118 VFISGQKDKVCEAKKYITRLVQ-----------TQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIEKRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNE---ISISGDRDGVAQAASILLQTYNQM 282 HSP 6 Score: 53.1434 bits (126), Expect = 1.885e-6 Identity = 34/118 (28.81%), Postives = 57/118 (48.31%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITRLVQT-----------QFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDI 569
V ++G + V AKK I V+ F ++L +P ++HR LIGNKG ++ S ++S P + I I+G KD +E A+ + V++ +V+ I+S I
Sbjct: 1068 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRI 1185
BLAST of Vigilin High density lipoprotein-binding protein vs. Planmine SMEST
Match: SMESG000006601.1 (SMESG000006601.1) HSP 1 Score: 2148.63 bits (5566), Expect = 0.000e+0 Identity = 1116/1118 (99.82%), Postives = 1116/1118 (99.82%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVXXXXXXXXXXEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSWRQIFPKSTIN 3913
KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKVVTIIGTPHGVEAVKAILLKTIETIENTKKKDIIFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYEKESNIIEIGIEISKNLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVPNKKSKSNPNEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTI SHVSAKPGEKPYMNGVDSSFSEEESFKSLSPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSWRQIFPKSTIN
Sbjct: 288 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKKVVTIIGTPHGVEAVKAILLKTIETIENTKKKDIIFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYEKESNIIEIGIEISKNLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGRDNMCKLNVPNKKSKSNPNEQGILEFRGPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTICYSHVSAKPGEKPYMNGVDSSFSEEESFKSLSPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHIPMPSQTSSWRQIFPKSTIN 1405 HSP 2 Score: 221.476 bits (563), Expect = 3.496e-58 Identity = 106/106 (100.00%), Postives = 106/106 (100.00%), Query Frame = 3
Query: 3 DNNYNNNNKHIQFSNDITELSLIHSQNPSKLVQFITDTFVIPYEERRYIDSESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQ 320
DNNYNNNNKHIQFSNDITELSLIHSQNPSKLVQFITDTFVIPYEERRYIDSESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQ
Sbjct: 36 DNNYNNNNKHIQFSNDITELSLIHSQNPSKLVQFITDTFVIPYEERRYIDSESNKLKKLLTEICANCGVRVETNVTPDQSLGVFISGQKDKVCEAKKYITRLVQTQ 141 HSP 3 Score: 163.696 bits (413), Expect = 2.699e-40 Identity = 80/83 (96.39%), Postives = 82/83 (98.80%), Query Frame = 3
Query: 312 QTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIE 560
+ +FEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIE
Sbjct: 205 ELKFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIE 287 HSP 4 Score: 82.0333 bits (201), Expect = 2.588e-15 Identity = 114/503 (22.66%), Postives = 236/503 (46.92%), Query Frame = 2
Query: 758 ELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIA---IDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLR---FEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETN--CSINFPR--DLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSK-KVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTI---LIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENV 2215
E+ ++IPKE H+ +IG +GS KE+ + TS V ++N+ S I + G KD + A L+ + + I ++ H+ KS + L + + + +++L F + N+I I G + V + + L + +++ +L++EI K+IIG G I++I +T+ IN R + + + ++G + ++ E++ + H +IG+ G L ++ P +++ F + +++ I + G ++V +V+ L+KK F ++S S+ +++I K IIG G+ I+++ K TN P + + I G VE A+K + + + I + HT+ LIG+ G + +K+ V I FP PDADS+ + + G + DV + L + SN+ + ++ + ++ + + +++L + + + + +E+ + I G++++V
Sbjct: 209 EISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSF--PKINDPS-TLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIEKRYHQLACHKSNTALHTV-LERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKF---IIGKSGSNLVDLSVNKPNIQLRF---EDEANQIVIEGSPEEVIEVSEYLKKK-VFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRDLTKK-VVTIIGTPHGVE-AVKAI--LLKTIETIENTKKKDIIFTHTLMPQLIGQQGKGLKEIKDRF-PEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDV 695 HSP 5 Score: 66.2402 bits (160), Expect = 1.697e-10 Identity = 41/179 (22.91%), Postives = 90/179 (50.28%), Query Frame = 2
Query: 2963 LDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEV-LNIDSRIHSRLIGSRGANLDQL 3496
++ +++P+EF+ +LIG KG K+L + P N T + + GS + + A++ I + V+E ++ ++ + + +YH+ + + + S+R+ +P + NEI+I G +D V +A + +L N++++ E+ + I H +IG RG+ + ++
Sbjct: 208 FEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQ--------VVEIEKRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEI 378 HSP 6 Score: 58.5362 bits (140), Expect = 4.185e-8 Identity = 56/248 (22.58%), Postives = 106/248 (42.74%), Query Frame = 2
Query: 2903 ITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVE----FVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIM 3634
I+G KDK+ AK+ I +L + + N EE L ++ + ++F V+ ++ +I E +EEL + E++ F ++L +P ++HR LIGNKG ++ S ++S P + I I+G KD +E A+ + V++ +V+ I+ R H L + ++ + + P + E + + G ++ V + A L+ N M
Sbjct: 120 ISGQKDKVCEAKKYITRL--VQTQTNCQEE-------------DVLTYVQHLNALYSDFETRFEDVLTMVIPPWIINPYGDIKETNVIILEELPEISTNEELK-FEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIEKRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNE---ISISGDRDGVAQAASILLQTYNQM 348 HSP 7 Score: 53.1434 bits (126), Expect = 1.633e-6 Identity = 34/118 (28.81%), Postives = 57/118 (48.31%), Query Frame = 3
Query: 249 VFISGQKDKVCEAKKYITRLVQT-----------QFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIESDI 569
V ++G + V AKK I V+ F ++L +P ++HR LIGNKG ++ S ++S P + I I+G KD +E A+ + V++ +V+ I+S I
Sbjct: 1134 VIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRI 1251
BLAST of Vigilin High density lipoprotein-binding protein vs. Planmine SMEST
Match: SMESG000016436.1 (SMESG000016436.1) HSP 1 Score: 495.352 bits (1274), Expect = 7.394e-164 Identity = 354/1111 (31.86%), Postives = 590/1111 (53.11%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRD----LTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNE--LFRIGRDNMCKLNVXXXXXXXXXXEQGILEFR--GPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHI 3856
KR++ +N+ ++ + E + +++IPP SV+ EI ISG++DGV QA I+ + Y K L + IPK +H Y+IG +GSGI EI ++T V +++ Q + +TI L GP + L PAL++V KSES+ + I+AP WLH+FIIGK GSNL L ++ + F + ++ IVIEG ++V E+ + K+ +L+S ++ E+++DP +HIIG G NIQ++++E I P + + + I G P GV+ K + +EN K KD+I H QLIG QG +KE+++++P+V I FP + SD++ L GPR+ VDQVA L K ++E+N++K VKI +YHK IIG+ G+ IR++ + N + P ++D+I +TGK+++VE+A KR+ + + +I E I I + LH LIG G I +++ CGG V I+FP+ D++S+ + I+G + DV KA+ +L +L E+ +++ E ++FL +L + R+ ++V + SE +K ++IIG KE V E L+ + + + + + +F K + + I R+ + I + R GP +++A I II++ E + ++ ++++ +N+ + ++ I Y V I+ P N +S+KPGE NG M E + NG+ NI E EV +T+ S P + I+G K+ A++ +++L + +V + EF+ IG KG+ I T H V + IP N + + G +++ AK + E + E KQ+R RN+ + VP +HR +IG +G I RTK +V I +P Q+S EITI+G++++V+KA+ E+++ V K ++ + E L+I +H +LIG+RG L +L+ ++KVEI FP R + E+A++V+V G KE+VD+C D LV+RAN +L+E +R Q+ E K+ + E + + GF + APW D +SS D ++ GD I
Sbjct: 211 KRFYPFINGSNNSKIYKIREMTNAKINIPPPSVESEEIIISGEKDGVYQALEIIQKIYEDTKKCSSSLNLPIPKSQHCYVIGAKGSGIAEIFERTGVSIEMPSQ---DSDDDTIVLYGPNEFLAPALSMVIQKSESMIRVNIEAPNWLHRFIIGKQGSNLNKLMEKYEHVFINFPENSDLIVIEGPKDDVNELKGQITHKIAELKSTMAITEIQVDPRFHRHIIGRSGANIQRMQEEYKVQIRMPGNNAVGTSGWTIRIEGDPEGVKLAKTEIESMSVKLENEKTKDVILDHKYHNQLIGAQGSRVKELRNQYPDVIIMFPDLSSKSDIVQLRGPRQQVDQVAVKLNKFIQEVIENNYTKDVKIFKQYHKNIIGKGGVNIRKIREEMNIRIDFPDEHSDSDKIRLTGKKENVEEAAKRIEAMQAELISIKEDTISIPQRLHISLIGTRGKLIKCIQQECGG-VQIQFPASDSNSDIIKIRGPESDVDKAKKQLLELSEKNLLLSHQDSVSAKSEFHKFLIGRKGAQLAEFRKRHAVMLIFPDSEDTNKDTIQIIGAKEAVQKAKEELEVTIKSLENEIEKYITIDTKWHKHFFKKRQAVMEEISRE----FGGVRIFFPKQDAVENIDQVRVIGPKGCVEAAVVRIEDIIENLEAQTSLSVFIEQKHHRNLITRNMMEIKSIQSNYDVTIKFP------------NRDLSSKPGE----NG-------------------------MEEIHLNGNGDVNI-ETMEEVDTTLE----------SEQNNPANWVTISGRKENCESAEDALLELVPITEDVPLANEFHRNFIGQKGQGINTFSTKHNVVVNIPPANKCSDFISIKGPRVNIIDAKAGLDELITMWENEKQDRIARNYENHVTVPIVFHRNIIGQRGANIEIFRTKFNVSIQIPDQNSKSEEITIRGYEEDVKKAEEELISMVEKWKAQVKEELHIPRDVHPQLIGTRGIALRKLRDQFKVEINFPNRNVDDEKAHLVEVIGDKENVDKCCDILVERANELLNEWEERNQVREVKNL-----IKDRENINSSSNIKEKGPGFLLTGAPWQQRVPD---ASSNVDFPKIDANGDKI 1253 HSP 2 Score: 103.99 bits (258), Expect = 7.394e-164 Identity = 56/158 (35.44%), Postives = 96/158 (60.76%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSESN------KLKKLLTEICANCGVRVETNV-TPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEI 557
+T T +P ERR+ + E+N KK+ + CGV +E N DQ+L VFISG +D V AK+ +T+ +QTQ ++ + +PKE+H+ ++G G+ +E+E+ ++T V+ P+ + + +I I G+K+GI+ AR I + V E SK+ + + I
Sbjct: 52 VTQTLTVPLAERRFSNREANNSVKNDAQKKICQTVAQKCGVSIELNSGNKDQNLMVFISGPQDNVSNAKRILTKELQTQTQLQMKVPKEYHKFILGRNGASLREIESGSNTNVTIPRSIENNDIITIFGTKEGIDKARAEINQIVAERSKLATERITI 209 HSP 3 Score: 149.443 bits (376), Expect = 5.379e-36 Identity = 138/579 (23.83%), Postives = 277/579 (47.84%), Query Frame = 2
Query: 602 LHTVLERKSLRLDIPPFSVDKN-EISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSN------LVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP-RDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIP---AASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLAL 2287
TV ++ + +++ + D+N + ISG +D V+ A IL + T +LQ+++PKE HK+I+G G+ ++EI ++ V I N + I + G K+ + A + + + + I P + FI G + S + + +N P + E+ +I+I G + V + E ++K D + S L + I ++IG+ G I +I + T SI P +D + + G + +++++ E++ + + H +IG+QG L ++ +++ V INFP +SDLI + GP+ DV+++ + K A L + ++++ ++H+ IIGR G I+ + + + ++P A I I G + V+ A + ++ K N + + H LIG G+R+ L+ V I FP + S+ V ++G + V + VKL+ ++E+ +NY + ++ + ++ + + ++K+RE ++RI E D +R+ G+KENV + ++ ++E+ I +T+++
Sbjct: 83 CQTVAQKCGVSIELNSGNKDQNLMVFISGPQDNVSNAKRILTKELQ----TQTQLQMKVPKEYHKFILGRNGASLREIESGSNTNVTIPRSIENN---DIITIFGTKEGIDKARAEINQIVAERSKLATERITIPKRFYPFINGSNNSKIYKIREMTNAKINIPPPSV----ESEEIIISGEKDGVYQALEIIQKIYEDTKKCSSSLNLPIPKSQHCYVIGAKGSGIAEIFERTGVSIEMPSQDSDDDTIVLYGPNEFLAPALSMVIQKSESMIRVNIEAPNWLHRF---IIGKQGSNLNKLMEKYEHVFINFP---ENSDLIVIEGPKDDVNELKGQITHKIAELKSTMAITEIQVDPRFHRHIIGRSGANIQRMQEEYKVQIRMPGNNAVGTSGWTIRIEGDPEGVKLAKTEIESMSVKLENEKTKDVILDHKYHNQLIGAQGSRVKELRNQYPD-VIIMFPDLSSKSDIVQLRGPRQQVDQVAVKLNKFIQEVIENNYTKDVKIFKQYHKNIIGKGGVNIRKIREEMNIRIDFPDEHSDSDKIRLTGKKENVEEAAKRIEAMQAELISIKEDTISI 643 HSP 4 Score: 148.673 bits (374), Expect = 9.441e-36 Identity = 180/732 (24.59%), Postives = 340/732 (46.45%), Query Frame = 2
Query: 1493 VRINFPPPDADSDLISLI-GPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLE-VLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXEQG-ILEFRGPVQNIKSAKDEIIHIIDDYENT--ITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHN---------ISEINSEVSSTISAGVAL-NKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFV---LDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNK-LESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
V I + D +L+ I GP+ +V IL K+ +++ ++K+P +YHK I+GR G ++RE+ +NT IP + E ND I I G ++ ++ A + I + S + I I K + + G ++I ++E ++ I P P +S +II G KD V +A + + E+ + + + + ++ + + ++ E V I S+ D + + G E + L V+QK+ S + ++I + + F IG+ N+ KL + N E ++ GP ++ K +I H I + ++T ITE + VD R ++I S + + ++Y+VQI++P + + S + + P GV + +E ES N D++ +H KYHN + E+ ++ I L +K I ++ P+ ++ D++ + + I+ + +V + ++++ +IG G I+K+R + I P+++S K+ +TG E+V A K I EL +K++ + +P + H LIG +G+ I I+ + V+I P+ DSN + I I+G + +V+KAK ++L K L + ++ S H LIG +GA L + +K++ V ++FP +E + +++ G KE+V + +EL
Sbjct: 92 VSIELNSGNKDQNLMVFISGPQDNVSNAKRILTKE----LQTQTQLQMKVPKEYHKFILGRNGASLREIESGSNTNVTIPRSIENNDIITIFGTKEGIDKARAEINQIVAERSKLATERITIPKRFYPFINGSNNSKIYKIREMTNAKINI--PPPSVESEEIIISGEKDGVYQALEIIQKIYEDTKKCSSSLNLPIPKSQHCYVIGAKGSGIAEIFERTGVSIEMPSQDSDDDTIVLYGPNEFLAPALSMVIQKSES--------MIRVNIEAPNWLHR----FIIGKQGSNLNKLMEKYEHVFINFPENSDLIVIEGPKDDVNELKGQITHKIAELKSTMAITE-IQVDPRFHRHIIGRSGANIQRMQEEYKVQIRMP------GNNAVGTSGWTIRIEGDP--EGVKLAKTEIESMSVKLENEK--TKDVILDH-----KYHNQLIGAQGSRVKELRNQYPDVIIMFPDLSSKSDIVQLRGPRQQV------DQVAVKLNKFIQEVIENNYTKDVKIFKQYHKNIIGKGGVNIRKIREEMNIRIDFPDEHSDSDKIRLTGKKENVEEAAKRIEAMQAELISIKED--------TISIPQRLHISLIGTRGKLIKCIQQECGGVQIQFPASDSNSDIIKIRGPESDVDKAKKQLLELSEKNLLLSHQDSVSAKSEFHKFLIGRKGAQLAEFRKRHAVMLIFP--DSEDTNKDTIQIIGAKEAVQKAKEEL 773 HSP 5 Score: 92.8189 bits (229), Expect = 3.873e-22 Identity = 147/633 (23.22%), Postives = 278/633 (43.92%), Query Frame = 2
Query: 533 NWTTSGRN*KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNII----AIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDE----ANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKE-TNCSINFPR-DLTKKV--VTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADS-------DLISLIGPR----KDVDQVAAILQKKH---------------------AFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDA---IKRVCNIYXXXXXXXXXXXXXXX-----XLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEA-----EDKSHL-RIIGQKENVLNTLEVLQKARSEM 2257
N+T + K+YH+ K + + E ++R+D P D ++I ++G ++ V +AA + ++ + +E I IP+ H +IG RG IK + Q V I + ++ + + I + GP+ ++ A + SE ++ ++ A HKF+IG+ G+ L + + + L F D + I I G+ E V + E L+ + L++ + + + ID KH +++I +E I FP+ D + + V +IG VEA + IE +E + LI + +K I+ + +V I FP D S + I L G + +++V L+ + A L ++ V + ++H+ IG++G I + N IP A++ +D I I G + ++ DA + + ++E E + H +IG+ G I + V+I+ P ++ S + I+G ++DV KA+ +L +VE+ A E+ + + + L + + L+KLR+ + V I+ ++K+HL +IG KENV ++L + +E+
Sbjct: 564 NYTKDVKIFKQYHKNIIGKGGVNIRKIREEMNIRIDFPDEHSDSDKIRLTGKKENVEEAAKRIEAMQAEL-ISIKEDTISIPQRLHISLIGTRGKLIK-CIQQECGGVQI-QFPASDSNSDIIKIRGPESDVDKAKKQLLELSEKNLLLSHQDSVSAKSEFHKFLIGRKGAQLAEFR-KRHAVMLIFPDSEDTNKDTIQIIGAKEAVQKAKEELEVTIKSLENEIEKY-ITIDTKWHKHFFKKRQAVMEEISREFGGVRIFFPKQDAVENIDQVRVIGPKGCVEAAVVRIEDIIENLEAQTSLSVFIEQKHHRNLITRNMMEIKSIQSNY-DVTIKFPNRDLSSKPGENGMEEIHLNGNGDVNIETMEEVDTTLESEQNNPANWVTISGRKENCESAEDALLELVPITEDVPLANEFHRNFIGQKGQGINTFSTKHNVVVNIPPANKCSDFISIKGPRVNIIDAKAGLDELITMWENEKQDRIARNYENHVTVPIVFHRNIIGQRGANIEIFRTKFN--VSIQIPDQNSKSEEITIRGYEEDVKKAEEELISMVEKWKAQVKEE-LHIPRDVHPQLIGTRGIALRKLRDQFKVEINFPNRNVDDEKAHLVEVIGDKENVDKCCDILVERANEL 1187 HSP 6 Score: 32.7278 bits (73), Expect = 3.873e-22 Identity = 39/171 (22.81%), Postives = 76/171 (44.44%), Query Frame = 3
Query: 45 NDITEL--SLIHSQNPSKLVQFITDTFVIPYEERRYIDSESNKLKKLLTEICANCGVRVETNVTPDQSLG-----VFISGQKDKVCEAKKYITRL-VQTQFEISLDI--PKEFHRSLIGNKGSLHKELENN-TSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDE 524
+D+ EL + H K IT+ V P R I ++++ E +V+ + + ++G + I G + V AK I + V+ + E + D+ ++H LIG +GS KEL N + FP ++ S +++++G + ++ + K + E
Sbjct: 395 DDVNELKGQITHKIAELKSTMAITEIQVDPRFHRHIIGRSGANIQRMQEE------YKVQIRMPGNNAVGTSGWTIRIEGDPEGVKLAKTEIESMSVKLENEKTKDVILDHKYHNQLIGAQGSRVKELRNQYPDVIIMFPDLSSKSDIVQLRGPRQQVDQVAVKLNKFIQE 559 HSP 7 Score: 83.5741 bits (205), Expect = 1.042e-15 Identity = 70/248 (28.23%), Postives = 127/248 (51.21%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFV----LDLE-VNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYK-VEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDR 3622
+ I G K+ I A+ EI ++ L E + +P+ FYP + G+ KI K+R I IP + + ++I++G + V A + I + E+ ++ SL L +P H +IG KG I++I + V I +PSQDS+ + I + G + + A + V + K ES I + + +H +IG +G+NL++L +KY+ V I FP E ++++ ++G K+ V+ ++ +
Sbjct: 176 ITIFGTKEGIDKARAEINQIVAERSKLATERITIPKRFYPFINGSNNSKIYKIREMTNAKINIPPPSVESEEIIISGEKDGVYQALEIIQKIYEDTKKCSS-------SLNLPIPKSQHCYVIGAKGSGIAEIFERTGVSIEMPSQDSDDDTIVLYGPNEFLAPALSMV---IQKSESMIRVNIEAPNWLHRFIIGKQGSNLNKLMEKYEHVFINFP------ENSDLIVIEGPKDDVNELKGQITHK 407
BLAST of Vigilin High density lipoprotein-binding protein vs. Planmine SMEST
Match: SMESG000016436.1 (SMESG000016436.1) HSP 1 Score: 494.967 bits (1273), Expect = 1.093e-163 Identity = 354/1111 (31.86%), Postives = 590/1111 (53.11%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFPRD----LTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI----SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLALSINDLTYFQKSNE--LFRIGRDNMCKLNVXXXXXXXXXXEQGILEFR--GPVQNIKSAKDEIIHIIDDYENTITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHNISEINSEVSSTISAGVALNKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFVLDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIMLDEINDRMQLEENKSSNYESNFQPEETPEPTKSCVTHKSGFQIRNAPWDAVGNDGNWSSSASDQNLVNGGGDHI 3856
KR++ +N+ ++ + E + +++IPP SV+ EI ISG++DGV QA I+ + Y K L + IPK +H Y+IG +GSGI EI ++T V +++ Q + +TI L GP + L PAL++V KSES+ + I+AP WLH+FIIGK GSNL L ++ + F + ++ IVIEG ++V E+ + K+ +L+S ++ E+++DP +HIIG G NIQ++++E I P + + + I G P GV+ K + +EN K KD+I H QLIG QG +KE+++++P+V I FP + SD++ L GPR+ VDQVA L K ++E+N++K VKI +YHK IIG+ G+ IR++ + N + P ++D+I +TGK+++VE+A KR+ + + +I E I I + LH LIG G I +++ CGG V I+FP+ D++S+ + I+G + DV KA+ +L +L E+ +++ E ++FL +L + R+ ++V + SE +K ++IIG KE V E L+ + + + + + +F K + + I R+ + I + R GP +++A I II++ E + ++ ++++ +N+ + ++ I Y V I+ P N +S+KPGE NG M E + NG+ NI E EV +T+ S P + I+G K+ A++ +++L + +V + EF+ IG KG+ I T H V + IP N + + G +++ AK + E + E KQ+R RN+ + VP +HR +IG +G I RTK +V I +P Q+S EITI+G++++V+KA+ E+++ V K ++ + E L+I +H +LIG+RG L +L+ ++KVEI FP R + E+A++V+V G KE+VD+C D LV+RAN +L+E +R Q+ E K+ + E + + GF + APW D +SS D ++ GD I
Sbjct: 213 KRFYPFINGSNNSKIYKIREMTNAKINIPPPSVESEEIIISGEKDGVYQALEIIQKIYEDTKKCSSSLNLPIPKSQHCYVIGAKGSGIAEIFERTGVSIEMPSQ---DSDDDTIVLYGPNEFLAPALSMVIQKSESMIRVNIEAPNWLHRFIIGKQGSNLNKLMEKYEHVFINFPENSDLIVIEGPKDDVNELKGQITHKIAELKSTMAITEIQVDPRFHRHIIGRSGANIQRMQEEYKVQIRMPGNNAVGTSGWTIRIEGDPEGVKLAKTEIESMSVKLENEKTKDVILDHKYHNQLIGAQGSRVKELRNQYPDVIIMFPDLSSKSDIVQLRGPRQQVDQVAVKLNKFIQEVIENNYTKDVKIFKQYHKNIIGKGGVNIRKIREEMNIRIDFPDEHSDSDKIRLTGKKENVEEAAKRIEAMQAELISIKEDTISIPQRLHISLIGTRGKLIKCIQQECGG-VQIQFPASDSNSDIIKIRGPESDVDKAKKQLLELSEKNLLLSHQDSVSAKSEFHKFLIGRKGAQLAEFRKRHAVMLIFPDSEDTNKDTIQIIGAKEAVQKAKEELEVTIKSLENEIEKYITIDTKWHKHFFKKRQAVMEEISRE----FGGVRIFFPKQDAVENIDQVRVIGPKGCVEAAVVRIEDIIENLEAQTSLSVFIEQKHHRNLITRNMMEIKSIQSNYDVTIKFP------------NRDLSSKPGE----NG-------------------------MEEIHLNGNGDVNI-ETMEEVDTTLE----------SEQNNPANWVTISGRKENCESAEDALLELVPITEDVPLANEFHRNFIGQKGQGINTFSTKHNVVVNIPPANKCSDFISIKGPRVNIIDAKAGLDELITMWENEKQDRIARNYENHVTVPIVFHRNIIGQRGANIEIFRTKFNVSIQIPDQNSKSEEITIRGYEEDVKKAEEELISMVEKWKAQVKEELHIPRDVHPQLIGTRGIALRKLRDQFKVEINFPNRNVDDEKAHLVEVIGDKENVDKCCDILVERANELLNEWEERNQVREVKNL-----IKDRENINSSSNIKEKGPGFLLTGAPWQQRVPD---ASSNVDFPKIDANGDKI 1255 HSP 2 Score: 103.99 bits (258), Expect = 1.093e-163 Identity = 56/158 (35.44%), Postives = 96/158 (60.76%), Query Frame = 3
Query: 105 ITDTFVIPYEERRYIDSESN------KLKKLLTEICANCGVRVETNV-TPDQSLGVFISGQKDKVCEAKKYITRLVQTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEI 557
+T T +P ERR+ + E+N KK+ + CGV +E N DQ+L VFISG +D V AK+ +T+ +QTQ ++ + +PKE+H+ ++G G+ +E+E+ ++T V+ P+ + + +I I G+K+GI+ AR I + V E SK+ + + I
Sbjct: 54 VTQTLTVPLAERRFSNREANNSVKNDAQKKICQTVAQKCGVSIELNSGNKDQNLMVFISGPQDNVSNAKRILTKELQTQTQLQMKVPKEYHKFILGRNGASLREIESGSNTNVTIPRSIENNDIITIFGTKEGIDKARAEINQIVAERSKLATERITI 211 HSP 3 Score: 149.443 bits (376), Expect = 6.445e-36 Identity = 138/579 (23.83%), Postives = 277/579 (47.84%), Query Frame = 2
Query: 602 LHTVLERKSLRLDIPPFSVDKN-EISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPA---LTLVFTKSESVNIIAIDAPGWLHKFIIGKSGSN------LVDLSVNKPNIQLRFEDEANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKETNCSINFP-RDLTKKVVTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADSDLISLIGPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIP---AASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLEVLQKARSEMGPIVTETLAL 2287
TV ++ + +++ + D+N + ISG +D V+ A IL + T +LQ+++PKE HK+I+G G+ ++EI ++ V I N + I + G K+ + A + + + + I P + FI G + S + + +N P + E+ +I+I G + V + E ++K D + S L + I ++IG+ G I +I + T SI P +D + + G + +++++ E++ + + H +IG+QG L ++ +++ V INFP +SDLI + GP+ DV+++ + K A L + ++++ ++H+ IIGR G I+ + + + ++P A I I G + V+ A + ++ K N + + H LIG G+R+ L+ V I FP + S+ V ++G + V + VKL+ ++E+ +NY + ++ + ++ + + ++K+RE ++RI E D +R+ G+KENV + ++ ++E+ I +T+++
Sbjct: 85 CQTVAQKCGVSIELNSGNKDQNLMVFISGPQDNVSNAKRILTKELQ----TQTQLQMKVPKEYHKFILGRNGASLREIESGSNTNVTIPRSIENN---DIITIFGTKEGIDKARAEINQIVAERSKLATERITIPKRFYPFINGSNNSKIYKIREMTNAKINIPPPSV----ESEEIIISGEKDGVYQALEIIQKIYEDTKKCSSSLNLPIPKSQHCYVIGAKGSGIAEIFERTGVSIEMPSQDSDDDTIVLYGPNEFLAPALSMVIQKSESMIRVNIEAPNWLHRF---IIGKQGSNLNKLMEKYEHVFINFP---ENSDLIVIEGPKDDVNELKGQITHKIAELKSTMAITEIQVDPRFHRHIIGRSGANIQRMQEEYKVQIRMPGNNAVGTSGWTIRIEGDPEGVKLAKTEIESMSVKLENEKTKDVILDHKYHNQLIGAQGSRVKELRNQYPD-VIIMFPDLSSKSDIVQLRGPRQQVDQVAVKLNKFIQEVIENNYTKDVKIFKQYHKNIIGKGGVNIRKIREEMNIRIDFPDEHSDSDKIRLTGKKENVEEAAKRIEAMQAELISIKEDTISI 645 HSP 4 Score: 148.673 bits (374), Expect = 1.131e-35 Identity = 180/732 (24.59%), Postives = 340/732 (46.45%), Query Frame = 2
Query: 1493 VRINFPPPDADSDLISLI-GPRKDVDQVAAILQKKHAFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDAIKRVCNIYXXXXXXXXXXXXXXXXLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRI---SEAEDKSHLRIIGQKENVLNTLE-VLQKARSEMGPIVTETLALSINDLTYFQKSNELFRIGR--DNMCKLNVXXXXXXXXXXEQG-ILEFRGPVQNIKSAKDEIIHIIDDYENT--ITENLIVDERQLKNIEWYSQNQMSEISQKYQVQIQIPFYPEPVNSKTISNSHVSAKPGEKPYMNGVDXXXXXXXXXXXXXPNPNHLPNDIMSEHNSNGSKYHN---------ISEINSEVSSTISAGVAL-NKDPISNIKLPQYKLEITGHKDKITLAKEEIIKLFV---LDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTK-NSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNK-LESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADEL 3613
V I + D +L+ I GP+ +V IL K+ +++ ++K+P +YHK I+GR G ++RE+ +NT IP + E ND I I G ++ ++ A + I + S + I I K + + G ++I ++E ++ I P P +S +II G KD V +A + + E+ + + + + ++ + + ++ E V I S+ D + + G E + L V+QK+ S + ++I + + F IG+ N+ KL + N E ++ GP ++ K +I H I + ++T ITE + VD R ++I S + + ++Y+VQI++P + + S + + P GV + +E ES N D++ +H KYHN + E+ ++ I L +K I ++ P+ ++ D++ + + I+ + +V + ++++ +IG G I+K+R + I P+++S K+ +TG E+V A K I EL +K++ + +P + H LIG +G+ I I+ + V+I P+ DSN + I I+G + +V+KAK ++L K L + ++ S H LIG +GA L + +K++ V ++FP +E + +++ G KE+V + +EL
Sbjct: 94 VSIELNSGNKDQNLMVFISGPQDNVSNAKRILTKE----LQTQTQLQMKVPKEYHKFILGRNGASLREIESGSNTNVTIPRSIENNDIITIFGTKEGIDKARAEINQIVAERSKLATERITIPKRFYPFINGSNNSKIYKIREMTNAKINI--PPPSVESEEIIISGEKDGVYQALEIIQKIYEDTKKCSSSLNLPIPKSQHCYVIGAKGSGIAEIFERTGVSIEMPSQDSDDDTIVLYGPNEFLAPALSMVIQKSES--------MIRVNIEAPNWLHR----FIIGKQGSNLNKLMEKYEHVFINFPENSDLIVIEGPKDDVNELKGQITHKIAELKSTMAITE-IQVDPRFHRHIIGRSGANIQRMQEEYKVQIRMP------GNNAVGTSGWTIRIEGDP--EGVKLAKTEIESMSVKLENEK--TKDVILDH-----KYHNQLIGAQGSRVKELRNQYPDVIIMFPDLSSKSDIVQLRGPRQQV------DQVAVKLNKFIQEVIENNYTKDVKIFKQYHKNIIGKGGVNIRKIREEMNIRIDFPDEHSDSDKIRLTGKKENVEEAAKRIEAMQAELISIKED--------TISIPQRLHISLIGTRGKLIKCIQQECGGVQIQFPASDSNSDIIKIRGPESDVDKAKKQLLELSEKNLLLSHQDSVSAKSEFHKFLIGRKGAQLAEFRKRHAVMLIFP--DSEDTNKDTIQIIGAKEAVQKAKEEL 775 HSP 5 Score: 92.8189 bits (229), Expect = 4.795e-22 Identity = 147/633 (23.22%), Postives = 278/633 (43.92%), Query Frame = 2
Query: 533 NWTTSGRN*KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQMKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSPNTICLLGPKDNLGPALTLVFTKSESVNII----AIDAPGWLHKFIIGKSGSNLVDLSVNKPNIQLRFEDE----ANQIVIEGSPEEVIEVSEYLKKKVFDLQSRLSQLEMEIDPLMIKHIIGSGGVNIQKIRKE-TNCSINFPR-DLTKKV--VTIIGTPHGVEAVKAILLXXXXXXXXXXXXXXXFTHTLMPQLIGQQGKGLKEIKDRFPEVRINFPPPDADS-------DLISLIGPR----KDVDQVAAILQKKH---------------------AFLVESNFSKKVKIPFKYHKAIIGREGITIRELTKATNTKFQIPAASEENDEIVITGKQKDVEDA---IKRVCNIYXXXXXXXXXXXXXXX-----XLHTILIGKGGNRINSLKESCGGRVTIRFPSPDADSNTVIIKGVKDDVLKAQVKLHDLVEEITASNYEQHFQMTLEDYEFLFMSADLKLKKLRETYSVRISEA-----EDKSHL-RIIGQKENVLNTLEVLQKARSEM 2257
N+T + K+YH+ K + + E ++R+D P D ++I ++G ++ V +AA + ++ + +E I IP+ H +IG RG IK + Q V I + ++ + + I + GP+ ++ A + SE ++ ++ A HKF+IG+ G+ L + + + L F D + I I G+ E V + E L+ + L++ + + + ID KH +++I +E I FP+ D + + V +IG VEA + IE +E + LI + +K I+ + +V I FP D S + I L G + +++V L+ + A L ++ V + ++H+ IG++G I + N IP A++ +D I I G + ++ DA + + ++E E + H +IG+ G I + V+I+ P ++ S + I+G ++DV KA+ +L +VE+ A E+ + + + L + + L+KLR+ + V I+ ++K+HL +IG KENV ++L + +E+
Sbjct: 566 NYTKDVKIFKQYHKNIIGKGGVNIRKIREEMNIRIDFPDEHSDSDKIRLTGKKENVEEAAKRIEAMQAEL-ISIKEDTISIPQRLHISLIGTRGKLIK-CIQQECGGVQI-QFPASDSNSDIIKIRGPESDVDKAKKQLLELSEKNLLLSHQDSVSAKSEFHKFLIGRKGAQLAEFR-KRHAVMLIFPDSEDTNKDTIQIIGAKEAVQKAKEELEVTIKSLENEIEKY-ITIDTKWHKHFFKKRQAVMEEISREFGGVRIFFPKQDAVENIDQVRVIGPKGCVEAAVVRIEDIIENLEAQTSLSVFIEQKHHRNLITRNMMEIKSIQSNY-DVTIKFPNRDLSSKPGENGMEEIHLNGNGDVNIETMEEVDTTLESEQNNPANWVTISGRKENCESAEDALLELVPITEDVPLANEFHRNFIGQKGQGINTFSTKHNVVVNIPPANKCSDFISIKGPRVNIIDAKAGLDELITMWENEKQDRIARNYENHVTVPIVFHRNIIGQRGANIEIFRTKFN--VSIQIPDQNSKSEEITIRGYEEDVKKAEEELISMVEKWKAQVKEE-LHIPRDVHPQLIGTRGIALRKLRDQFKVEINFPNRNVDDEKAHLVEVIGDKENVDKCCDILVERANEL 1189 HSP 6 Score: 32.7278 bits (73), Expect = 4.795e-22 Identity = 39/171 (22.81%), Postives = 76/171 (44.44%), Query Frame = 3
Query: 45 NDITEL--SLIHSQNPSKLVQFITDTFVIPYEERRYIDSESNKLKKLLTEICANCGVRVETNVTPDQSLG-----VFISGQKDKVCEAKKYITRL-VQTQFEISLDI--PKEFHRSLIGNKGSLHKELENN-TSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDE 524
+D+ EL + H K IT+ V P R I ++++ E +V+ + + ++G + I G + V AK I + V+ + E + D+ ++H LIG +GS KEL N + FP ++ S +++++G + ++ + K + E
Sbjct: 397 DDVNELKGQITHKIAELKSTMAITEIQVDPRFHRHIIGRSGANIQRMQEE------YKVQIRMPGNNAVGTSGWTIRIEGDPEGVKLAKTEIESMSVKLENEKTKDVILDHKYHNQLIGAQGSRVKELRNQYPDVIIMFPDLSSKSDIVQLRGPRQQVDQVAVKLNKFIQE 561 HSP 7 Score: 83.5741 bits (205), Expect = 1.061e-15 Identity = 70/248 (28.23%), Postives = 127/248 (51.21%), Query Frame = 2
Query: 2897 LEITGHKDKITLAKEEIIKLFV----LDLE-VNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYK-VEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDR 3622
+ I G K+ I A+ EI ++ L E + +P+ FYP + G+ KI K+R I IP + + ++I++G + V A + I + E+ ++ SL L +P H +IG KG I++I + V I +PSQDS+ + I + G + + A + V + K ES I + + +H +IG +G+NL++L +KY+ V I FP E ++++ ++G K+ V+ ++ +
Sbjct: 178 ITIFGTKEGIDKARAEINQIVAERSKLATERITIPKRFYPFINGSNNSKIYKIREMTNAKINIPPPSVESEEIIISGEKDGVYQALEIIQKIYEDTKKCSS-------SLNLPIPKSQHCYVIGAKGSGIAEIFERTGVSIEMPSQDSDDDTIVLYGPNEFLAPALSMV---IQKSESMIRVNIEAPNWLHRFIIGKQGSNLNKLMEKYEHVFINFP------ENSDLIVIEGPKDDVNELKGQITHK 409
BLAST of Vigilin High density lipoprotein-binding protein vs. Planmine SMEST
Match: SMESG000018596.1 (SMESG000018596.1) HSP 1 Score: 164.081 bits (414), Expect = 2.252e-72 Identity = 80/83 (96.39%), Postives = 82/83 (98.80%), Query Frame = 3
Query: 312 QTQFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIE 560
+ +FEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIE
Sbjct: 193 ELKFEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIE 275 HSP 2 Score: 130.183 bits (326), Expect = 2.252e-72 Identity = 61/61 (100.00%), Postives = 61/61 (100.00%), Query Frame = 2
Query: 560 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQM 742
KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQM
Sbjct: 276 KRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQM 336 HSP 3 Score: 57.3806 bits (137), Expect = 3.422e-8 Identity = 34/149 (22.82%), Postives = 74/149 (49.66%), Query Frame = 2
Query: 2963 LDLEVNVPEEFYPALIGAKGEKIKKLRTTHQVDIKIPNKNSKFTKVIVTGSSESVVAAKKEIVEFVEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKL 3409
++ +++P+EF+ +LIG KG K+L + P N T + + GS + + A++ I + V+E ++ ++ + + +YH+ + + + S+R+ +P + NEI+I G +D V +A + +L N++
Sbjct: 196 FEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQ--------VVEIEKRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNEISISGDRDGVAQAASILLQTYNQM 336 HSP 4 Score: 55.8398 bits (133), Expect = 1.144e-7 Identity = 39/159 (24.53%), Postives = 74/159 (46.54%), Query Frame = 2
Query: 3158 VEELERMKQEREIRNFSLALHVPAQYHRKLIGNKGETISKIRTKNSVRISVPSQDSNINEITIQGHKDNVEKAKNEVLAFVNKLESGIIEVLNIDSRIHSRLIGSRGANLDQLKKKYKVEIVFPIRGTEGEEANIVKVKGTKESVDRCADELVDRANIM 3634
+EEL + E++ F ++L +P ++HR LIGNKG ++ S ++S P + I I+G KD +E A+ + V++ +V+ I+ R H L + ++ + + P + E + + G ++ V + A L+ N M
Sbjct: 182 LEELPEISTNEELK-FEISLDIPKEFHRSLIGNKGSLHKELENNTSTQVSFPKINDPSTLIKIKGSKDGIEHARQLIQKNVDEFSKIGRQVVEIEKRYHQLACHKSNTALHTVLERKSLRLDIPPFSVDKNE---ISISGDRDGVAQAASILLQTYNQM 336 The following BLAST results are available for this feature:
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Vigilin High density lipoprotein-binding protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Vigilin High density lipoprotein-binding protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 3
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 2
BLAST of Vigilin High density lipoprotein-binding protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Vigilin High density lipoprotein-binding protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30013430 ID=SMED30013430|Name=Vigilin High density lipoprotein-binding protein|organism=Schmidtea mediterranea sexual|type=transcript|length=4090bpback to top protein sequence of SMED30013430-orf-1 >SMED30013430-orf-1 ID=SMED30013430-orf-1|Name=SMED30013430-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=192bp DNNYNNNNKHIQFSNDITELSLIHSQNPSKLVQFITDTFVIPYEERRYIDback to top protein sequence of SMED30013430-orf-2 >SMED30013430-orf-2 ID=SMED30013430-orf-2|Name=SMED30013430-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1059bp MKATYQELQIEIPKEKHKYIIGPRGSGIKEILDQTSVWVDINEQRLNEGSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
