SMED30013404

Overview
NameSMED30013404
Smed IDSMED30013404
Length (bp)1237
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30013404 (SMED30013404) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30013404 (SMED30013404) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30013404 (SMED30013404) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30013404 (SMED30013404) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013404

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30013404h1SMcG0003224 Contig2140newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30013404h1SMcG0003224 Contig2140uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30013404h1SMcG0002776 Contig2140newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30013404h1SMcG0002776 Contig2140uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
head regionSMED30013404h1SMcG0003224 SmedASXL_012844SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30013404h1SMcG0003224 dd_Smed_v4_16528_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30013404h1SMcG0003224 dd_Smed_v4_16528_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30013404h1SMcG0003224 dd_Smed_v4_16528_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30013404h1SMcG0003224 dd_Smed_v4_16528_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30013404h1SMcG0003224 dd_Smed_v6_16528_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30013404h1SMcG0003224 dd_Smed_v4_16528_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30013404 vs. TrEMBL
Match: A0A267FJD5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027923g1 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 5.217e-15
Identity = 69/302 (22.85%), Postives = 148/302 (49.01%), Query Frame = 3 
Query:  105 LTPNEIVLTYRIQLKEEKLTNLKFELKKIYEDNVQINNKLKSFEQDKESFCNEMLRKYRESDGKASKSNVYKKSDVVNRMKSNWAISKNEQNXXXXXXXXXXXXXXXXXXXXXXXXNLKYMKNCELEDNDQQIKILKHILDDMENNFNAIKSYLEKTYYDKKSELVDNEKLSTEKFQKDACIKASKVIKDNMKQEITDNKWLINKRSLHRENFERLKSEIGIIENENIQLNEKIFQLKQSEKKFA-NLFITEIEPDEILFNQGILEYDFNKVFKFYPKKNSELDENENSEIYSTSSFEKRID 1007
            +TP E +  Y+I+L+E +L  + F++K   E   +   + K   +++E++ N +L++ +E + K S+ +   + +VV  M+ NW + + E+ EI  L+  IE          SEI   +  ++    +    I++L+  + D++  F ++  YL++     +  +      + E+ +  A   A + +  N KQE+ DN WL  +   HR   ER+ +++  +E  N+++   +F+ +  + +F+ + F+T+    + L   G+LE D ++V           D  + +     S+ EK ++
Sbjct:   27 MTPAEAIWDYKIKLQERELEEIAFQVKGQLEMKERNQERNKRLAEEREAYMNMLLKRLQEQEKKDSQ-HFTDRPEVVAAMQENWRLEQQEREEIRSLRQEIEQTIADTAATRSEIETWREFRDRGQLEQQTSIRLLRQEVRDIDETFKSMCEYLDRKTRQDRDRIQTTTDTALEEQKSRATDTAVQQLDKNTKQELVDNSWLKEEAETHRAEMERVLAQVEDLERRNLRIMADLFECRVQDLRFSRDFFLTQFRDGDSLAEPGLLELDLDQVLASKGAIGGAGDSEDGASRQPRSASEKALE 327
[Close]
BLAST of SMED30013404 vs. TrEMBL
Match: A0A267FS54 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig020312g1 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 6.216e-15
Identity = 69/302 (22.85%), Postives = 148/302 (49.01%), Query Frame = 3 
Query:  105 LTPNEIVLTYRIQLKEEKLTNLKFELKKIYEDNVQINNKLKSFEQDKESFCNEMLRKYRESDGKASKSNVYKKSDVVNRMKSNWAISKNEQNXXXXXXXXXXXXXXXXXXXXXXXXNLKYMKNCELEDNDQQIKILKHILDDMENNFNAIKSYLEKTYYDKKSELVDNEKLSTEKFQKDACIKASKVIKDNMKQEITDNKWLINKRSLHRENFERLKSEIGIIENENIQLNEKIFQLKQSEKKFA-NLFITEIEPDEILFNQGILEYDFNKVFKFYPKKNSELDENENSEIYSTSSFEKRID 1007
            +TP E +  Y+I+L+E +L  + F++K   E   +   + K   +++E++ N +L++ +E + K S+ +   + +VV  M+ NW + + E+ EI  L+  IE          SEI   +  ++    +    I++L+  + D++  F ++  YL++     +  +      + E+ +  A   A + +  N KQE+ DN WL  +   HR   ER+ +++  +E  N+++   +F+ +  + +F+ + F+T+    + L   G+LE D ++V           D  + +     S+ EK ++
Sbjct:   27 MTPAEAIWDYKIKLQERELEEIAFQVKGQLEMKERNQERNKRLAEEREAYMNMLLKRLQEQEKKDSE-HFTDRPEVVAAMQENWRLDQQEREEIRSLRQEIEQTIADTAATRSEIETWREFRDRGQLEQQTSIRLLRQEVRDIDETFKSMCEYLDRKTRQDRDRIQTTTDTALEEQKSRATDTAVQQLDKNTKQELVDNSWLKEEAETHRAEMERVLAQVEDLERRNLRIMADLFECRVQDLRFSRDFFLTQFRDGDSLAEPGLLELDLDQVLASKGAIGGTGDSEDGASRQPRSASEKALE 327
[Close]
BLAST of SMED30013404 vs. TrEMBL
Match: A0A267DUY9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027923g2 PE=4 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.424e-14
Identity = 69/302 (22.85%), Postives = 147/302 (48.68%), Query Frame = 3 
Query:  105 LTPNEIVLTYRIQLKEEKLTNLKFELKKIYEDNVQINNKLKSFEQDKESFCNEMLRKYRESDGKASKSNVYKKSDVVNRMKSNWAISKNEQNXXXXXXXXXXXXXXXXXXXXXXXXNLKYMKNCELEDNDQQIKILKHILDDMENNFNAIKSYLEKTYYDKKSELVDNEKLSTEKFQKDACIKASKVIKDNMKQEITDNKWLINKRSLHRENFERLKSEIGIIENENIQLNEKIFQLKQSEKKFA-NLFITEIEPDEILFNQGILEYDFNKVFKFYPKKNSELDENENSEIYSTSSFEKRID 1007
            +TP E +  Y+I+L+E +L  + F++K   E   +   + K   +++E++ N +L++ +E + K S+ +   +  VV  M+ NW + + E+ EI  L+  IE          SEI   +  ++    +    I++L+  + D++  F ++  YL++     +  +      + E+ +  A   A + +  N KQE+ DN WL  +   HR   ER+ +++  +E  N+++   +F+ +  + +F+ + F+T+    + L   G+LE D ++V           D  + +     S+ EK ++
Sbjct:   27 MTPAEAIWDYKIKLQERELEEIAFQVKGQLEMKERNQERNKRLAEEREAYMNMLLKRLQEQEKKDSE-HFTDRPKVVAAMQENWRLEQQEREEIRSLRQEIEQTIADTAATRSEIETWREFRDRGQLEQQTSIRLLRQEVRDIDETFKSMCEYLDRKTRQDRDRIQTTTDTALEEQKSRATDTAVQQLDKNTKQELVDNSWLKEEAETHRAEMERVLAQVEDLERRNLRIMADLFECRVQDLRFSRDFFLTQFRDGDSLAEPGLLELDLDQVLASKGAIGGAGDSEDGASRQPRSASEKALE 327
[Close]
BLAST of SMED30013404 vs. TrEMBL
Match: A0A1W2WNR1 (coiled-coil domain-containing protein 83-like OS=Ciona intestinalis OX=7719 GN=LOC100180806 PE=4 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 9.979e-12
Identity = 83/353 (23.51%), Postives = 167/353 (47.31%), Query Frame = 3 
Query:  105 LTPNEIVLTYRIQLKEEKLTNLKFELKKIYEDNVQINNKLKSFEQDKESFCNEMLRKYRESDGKASKSNVYKKSDVVNRMKSNWAISKNEQNXXXXXXXXXXXXXXXXXXXXXXXXNLKYMKNCELEDNDQQIKILKHILDDMENNFNAIKSYLEKTYYDKKSELVDNEKLSTEKFQKDACIKASKVIKDNMKQEITDNKWLINKRSLHRENFERLKSEIGIIENENIQLNEKIFQLKQSEKKFA-NLFITEIEPDEILFNQGILEYDF-------------NKVFKFYPKKNSELDENENSEIYSTSSFEKRI--------------DLSYSSWKDYLNLNPVNFDFLYISG 1079
            +T  E +L ++IQ+KE+   +L+FE+K + + N +  ++     +D++     +LR+ +E + +  +  VY    V   ++  WA ++NE  E+ DL   IE  +++  ++ S++      K+   E++ +QI +L   L+DM  NFN +  +L +     K E+   ++    + Q  A  KA + +     QE+ +N+WL  +  +HRE  + L   +  +E+EN+ +  ++   KQ + K + N ++T+ E DEIL    + + D              N V    P+  S +      +++S    ++                D++    KD+L+L P+    L ++G
Sbjct:   28 MTLKEAILAFQIQVKEKASEDLQFEIKGLKDKNRRFRDRNARLYEDQQIHIKTLLRQAKEQEHELEEKEVYNSEHVEQALREKWAAARNEDKELDDLYKRIEQVEQEYAKMSSDVEYWTEYKDVGSEEHAKQIHLLNSELEDMLRNFNDMCGHLTRQLEAAKYEVTLYKEECMAEQQHLASEKAMEHLDPASVQEVHENEWLKKEAHIHREEEKNLLRLLDELESENLSIISELLYCKQEDLKISRNFYMTQFEEDEILNQDTMHDMDLKQMSVYQTKDDDNNSVKAIEPRPMSAMQRAVEQKVFSILPEQEEEEEEWENEIIARQDQDINIYEEKDFLHLGPLEIKLLCVTG 380
[Close]
BLAST of SMED30013404 vs. TrEMBL
Match: F6ZDH4 (Uncharacterized protein OS=Ciona intestinalis OX=7719 PE=4 SV=2)

HSP 1 Score: 73.9442 bits (180), Expect = 1.191e-10
Identity = 71/278 (25.54%), Postives = 144/278 (51.80%), Query Frame = 3 
Query:  105 LTPNEIVLTYRIQLKEEKLTNLKFELKKIYEDNVQINNKLKSFEQDKESFCNEMLRKYRESDGKASKSNVYKKSDVVNRMKSNWAISKNEQNXXXXXXXXXXXXXXXXXXXXXXXXNLKYMKNCELEDNDQQIKILKHILDDMENNFNAIKSYLEKTYYDKKSELVDNEKLSTEKFQKDACIKASKVIKDNMKQEITDNKWLINKRSLHRENFERLKSEIGIIENENIQLNEKIFQLKQSEKKFA-NLFITEIEPDEILFNQGILEYDFNKVFKFYPK 935
            +T  E +L ++IQ+KE+   +L+FE+K + + N +  ++     +D++     +LR+ +E + +  +  VY    V   ++  WA ++NE  E+ DL   IE  +++  ++ S++      K+   E++ +QI +L   L+DM  NFN +  +L +     K E+   ++    + Q  A  KA + +     QE+ +N+WL  +  +HRE  + L   +  +E+EN+ +  ++   KQ + K + N ++T+ E DEIL    + + D  ++  +  K
Sbjct:   28 MTLKEAILAFQIQVKEKASEDLQFEIKGLKDKNRRFRDRNARLYEDQQIHIKTLLRQAKEQEHELEEKEVYNSEHVEQALREKWAAARNEDKELDDLYKRIEQVEQEYAKMSSDVEYWTEYKDVGSEEHAKQIHLLNSELEDMLRNFNDMCGHLTRQLEAAKYEVTLYKEECMAEQQHLASEKAMEHLDPASVQEVHENEWLKKEAHIHREEEKNLLRLLDELESENLSIISELLYCKQEDLKISRNFYMTQFEEDEILNQDTMHDMDLKQMSVYQTK 305
[Close]
BLAST of SMED30013404 vs. Planmine SMEST
Match: SMESG000027863.1 (SMESG000027863.1)

HSP 1 Score: 614.764 bits (1584), Expect = 0.000e+0
Identity = 387/389 (99.49%), Postives = 387/389 (99.49%), Query Frame = 3 
Query:   30 MPPXXXXXXXXXXXXXXXXXXXXXXLTPNEIVLTYRIQLKEEKLTNLKFELKKIYEDNVQINNKLKSFEQDKESFCNEMLRKYRESDGKASKSNVYKKSDVVNRMKSNWAISKNEQNXXXXXXXXXXXXXXXXXXXXXXXXNLKYMKNCELEDNDQQIKILKHILDDMENNFNAIKSYLEKTYYDKKSELVDNEKLSTEKFQKDACIKASKVIKDNMKQEITDNKWLINKRSLHRENFERLKSEIGIIENENIQLNEKIFQLKQSEKKFANLFITEIEPDEILFNQGILEYDFNKVFKFYPKKNSELDENENSEIYSTSSFEKRIDLSYSSWKDYLNLNPVNFDFLYISGNLLDIQEAXXXXXXXXXXXXXXXXXXPVDIHSLKDNVII 1196
            MPPKKGKT KKSKKGTKNNSIKKISLTPNEIVLTYRIQLKEEKLTNLKFELKKIYEDNVQINNKLKSFEQDKESFCNEMLR YRESDGKASKSNVYKKSDVVNRMKSNWAISKNEQNEIIDLKIYIEDKDKKIKELESEISNLKYMKNCELEDNDQQIKILKHILDDMENNFNAIKSYLEKTYYDKKSELVDNEKLSTEKFQKDACIKASKVIKDNMKQEITDNKWLINKRSLHRENFERLKSEIGIIENENIQLNEKIFQLKQSEKKFANLFITEIEPDEILFNQGILEYDFNKVFKFYPKKNSELDENENSEIYSTSSFEKRIDLSYSSWKDYLNLNPVNFDFLYISGNLLDIQEAKPKTKEEMEEEMFTIKKFPVDIHSLKDNVII
Sbjct:    1 MPPKKGKTGKKSKKGTKNNSIKKISLTPNEIVLTYRIQLKEEKLTNLKFELKKIYEDNVQINNKLKSFEQDKESFCNEMLRNYRESDGKASKSNVYKKSDVVNRMKSNWAISKNEQNEIIDLKIYIEDKDKKIKELESEISNLKYMKNCELEDNDQQIKILKHILDDMENNFNAIKSYLEKTYYDKKSELVDNEKLSTEKFQKDACIKASKVIKDNMKQEITDNKWLINKRSLHRENFERLKSEIGIIENENIQLNEKIFQLKQSEKKFANLFITEIEPDEILFNQGILEYDFNKVFKFYPKKNSELDENENSEIYSTSSFEKRIDLSYSSWKDYLNLNPVNFDFLYISGNLLDIQEAKPKTKEEMEEEMFTIKKFPVDIHSLKDNVII 389
The following BLAST results are available for this feature:
BLAST of SMED30013404 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013404 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013404 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013404 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013404 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013404 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013404 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013404 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267FJD55.217e-1522.85Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FS546.216e-1522.85Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DUY91.424e-1422.85Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1W2WNR19.979e-1223.51coiled-coil domain-containing protein 83-like OS=C... [more]
F6ZDH41.191e-1025.54Uncharacterized protein OS=Ciona intestinalis OX=7... [more]
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BLAST of SMED30013404 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013404 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013404 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013404 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013404 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013404 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000027863.10.000e+099.49SMESG000027863.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013404 ID=SMED30013404|Name=SMED30013404|organism=Schmidtea mediterranea sexual|type=transcript|length=1237bp
TTCATAGTAACAATTATAAAAACATTTGAATGCCACCTAAAAAGGGTAAA
ACGAGCAAAAAAAGCAAGAAAGGTACAAAAAATAATTCGATCAAAAAAAT
TTCACTTACTCCAAATGAAATAGTATTGACTTATCGAATTCAATTGAAGG
AAGAAAAATTAACTAACTTAAAATTTGAGCTTAAAAAAATTTATGAAGAT
AATGTTCAAATCAATAATAAACTTAAAAGTTTCGAACAAGACAAAGAAAG
TTTTTGTAATGAAATGCTCAGAAAGTATCGTGAATCGGACGGCAAAGCTT
CCAAAAGTAATGTTTATAAAAAATCTGATGTTGTAAACCGCATGAAATCC
AATTGGGCAATTTCAAAGAATGAGCAAAATGAAATCATTGATTTAAAAAT
CTATATTGAAGATAAAGACAAGAAAATTAAAGAGCTTGAATCTGAAATAT
CAAATTTAAAATACATGAAAAATTGTGAACTTGAAGATAATGATCAACAA
ATCAAAATTTTGAAACATATTTTAGATGACATGGAAAATAATTTTAATGC
TATAAAGTCTTATCTTGAAAAGACATATTATGATAAAAAATCTGAGTTAG
TAGATAATGAAAAACTGTCGACGGAAAAGTTTCAGAAAGATGCTTGTATT
AAAGCATCTAAAGTAATCAAAGACAACATGAAGCAGGAGATCACAGATAA
CAAATGGCTGATTAATAAAAGAAGCCTTCACAGAGAAAATTTTGAAAGAC
TCAAATCTGAGATTGGAATTATAGAAAACGAGAATATTCAACTAAATGAA
AAAATATTTCAATTAAAACAATCGGAGAAAAAATTTGCAAATTTATTTAT
AACAGAAATAGAGCCAGATGAAATTTTGTTCAATCAAGGAATTTTGGAAT
ATGACTTCAACAAAGTTTTCAAATTTTATCCAAAAAAAAACAGTGAACTT
GATGAAAATGAAAATTCAGAAATATATTCAACAAGTTCCTTCGAAAAAAG
AATTGATTTATCTTATTCCAGCTGGAAAGATTATTTAAATTTGAATCCAG
TGAATTTCGATTTTTTATATATTTCGGGAAACTTATTAGATATTCAAGAA
GCAAAACCGAAAACTAAAGAAGAAATGGAAGAAGAAATGTTTACTATTAA
AAAATTTCCTGTAGATATTCATTCACTTAAAGATAATGTAATAATTTGAT
ATTTTGTTTTATTAAAAATATAAAAGTTGTTAATATG
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protein sequence of SMED30013404-orf-1

>SMED30013404-orf-1 ID=SMED30013404-orf-1|Name=SMED30013404-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=390bp
MPPKKGKTSKKSKKGTKNNSIKKISLTPNEIVLTYRIQLKEEKLTNLKFE
LKKIYEDNVQINNKLKSFEQDKESFCNEMLRKYRESDGKASKSNVYKKSD
VVNRMKSNWAISKNEQNEIIDLKIYIEDKDKKIKELESEISNLKYMKNCE
LEDNDQQIKILKHILDDMENNFNAIKSYLEKTYYDKKSELVDNEKLSTEK
FQKDACIKASKVIKDNMKQEITDNKWLINKRSLHRENFERLKSEIGIIEN
ENIQLNEKIFQLKQSEKKFANLFITEIEPDEILFNQGILEYDFNKVFKFY
PKKNSELDENENSEIYSTSSFEKRIDLSYSSWKDYLNLNPVNFDFLYISG
NLLDIQEAKPKTKEEMEEEMFTIKKFPVDIHSLKDNVII*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR026702CCDC83
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 119..146
NoneNo IPR availableCOILSCoilCoilcoord: 238..258
NoneNo IPR availableCOILSCoilCoilcoord: 51..71
NoneNo IPR availableCOILSCoilCoilcoord: 151..171
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
IPR026702Coiled-coil domain-containing protein 83PANTHERPTHR21468HSD9coord: 1..325