Pre-mRNA-splicing ATP-dependent RNA helicase PRP28

Overview


Name	Pre-mRNA-splicing ATP-dependent RNA helicase PRP28
Smed ID	SMED30013392
Length (bp)	4372

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 (SMED30013392) t-SNE clustered cells

Violin plots show distribution of expression levels for Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 (SMED30013392) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 (SMED30013392) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30013392

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 11

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X1 cell	SMED30013392	SMESG000068237.1	SmedASXL_011090	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
X2 cell	SMED30013392	SMESG000068237.1	SmedASXL_011090	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
pharynx	SMED30013392	SMESG000068237.1	dd_Smed_v4_5080_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30013392	SMESG000068237.1	dd_Smed_v4_5080_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30013392	SMESG000068237.1	dd_Smed_v4_5080_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30013392	SMESG000068237.1	dd_Smed_v4_5080_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30013392	SMESG000068237.1	dd_Smed_v4_5080_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30013392	SMESG000068237.1	dd_Smed_v4_5080_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30013392	SMESG000068237.1	dd_Smed_v4_5080_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30013392	SMESG000068237.1	dd_Smed_v4_5080_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30013392	SMESG000068237.1	dd_Smed_v6_5080_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Human
Match: DDX23 (DEAD-box helicase 23 [Source:HGNC Symbol;Acc:HGNC:17347])

HSP 1 Score: 746.888 bits (1927), Expect = 0.000e+0
Identity = 364/570 (63.86%), Postives = 461/570 (80.88%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            RYLG  +  +R +   N+RKFVF WD  EDTS DYNPLYK++H+ Q  GRG I GID+  QK+   +FY  +M  RR+  +K+QE  RL+ +  +EAK +WD+R W++K LDEMT+RDWRIFRED+SITTKGG IPN IR W+++ L   +L+VI +K  YKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL WI  LP++ ++E+++ GPYAIIL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G EIVIATPGRL+DVLENRY+VL++CTYV++DE DRMIDM FE DV+KIL ++P + +KPDTD+AED EK+L NF +   KYRQTVMFTATM P+VERLAR++LRRPA VYIGS GKP ER +Q +++++E++KR KL  I+ + F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI+DVS+V+NYDM K I++YIHR+GRTGRAGK G+AI+F+TK+DS +FY+LK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:  253 RYLGGIKKRRRTRHL-NDRKFVFEWDASEDTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEARLRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWKDSSLPPHILEVI-DKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEHMPVSNQKPDTDEAEDPEKMLANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSESEKRKKLLAILEQGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSAVFYELKQAILESPVSSCPPELANHPDAQHKPGTILTKKRR-EETIF 819

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Human
Match: DDX46 (DEAD-box helicase 46 [Source:HGNC Symbol;Acc:HGNC:18681])

HSP 1 Score: 280.026 bits (715), Expect = 3.119e-77
Identity = 161/420 (38.33%), Postives = 244/420 (58.10%), Query Frame = 1

Query: 1024 HLDEMTNRDWRIFREDF-SITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVL---ENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKP---TERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDS 2262
             L +M+  +  +FR +   IT KG   P  I+ W + G+S K+L+ +  K  Y++PTPIQ QAIP  +  RD+IG+A+TGSGKT AFL+P+   I     L++ E    GP A+I+ PTR+LA+QI +E  KF + LG++ V + GG    EQ+ +L+ GAEI++ TPGR++D+L     R   L + TYV++DE DRM DM FE  V +I+  +     +PD                    RQTVMF+AT   ++E LAR  L +P  V +G  G+    ++  +QVI +  E +    L  +        +IIFV++++ AD L K L + +   +S HGG  Q  R+  +   K+    +LVAT++A RG+D+K + LV+NY  P   ++Y+HR GRTGRAG +G A +F+T+D +
Sbjct:  341 ELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSL-KKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGE----GPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNV-----RPD--------------------RQTVMFSATFPRAMEALARRILSKPIEVQVG--GRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQA 728

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Human
Match: DDX46 (DEAD-box helicase 46 [Source:HGNC Symbol;Acc:HGNC:18681])

HSP 1 Score: 280.026 bits (715), Expect = 3.167e-77
Identity = 161/420 (38.33%), Postives = 244/420 (58.10%), Query Frame = 1

Query: 1024 HLDEMTNRDWRIFREDF-SITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVL---ENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKP---TERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDS 2262
             L +M+  +  +FR +   IT KG   P  I+ W + G+S K+L+ +  K  Y++PTPIQ QAIP  +  RD+IG+A+TGSGKT AFL+P+   I     L++ E    GP A+I+ PTR+LA+QI +E  KF + LG++ V + GG    EQ+ +L+ GAEI++ TPGR++D+L     R   L + TYV++DE DRM DM FE  V +I+  +     +PD                    RQTVMF+AT   ++E LAR  L +P  V +G  G+    ++  +QVI +  E +    L  +        +IIFV++++ AD L K L + +   +S HGG  Q  R+  +   K+    +LVAT++A RG+D+K + LV+NY  P   ++Y+HR GRTGRAG +G A +F+T+D +
Sbjct:  341 ELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSL-KKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGE----GPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNV-----RPD--------------------RQTVMFSATFPRAMEALARRILSKPIEVQVG--GRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQA 728

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Human
Match: DDX43 (DEAD-box helicase 43 [Source:HGNC Symbol;Acc:HGNC:18677])

HSP 1 Score: 266.544 bits (680), Expect = 1.559e-75
Identity = 163/450 (36.22%), Postives = 257/450 (57.11%), Query Frame = 1

Query:  979 REAKVKWDERPWTE---------------KHLDEMTNRDWRIFREDFSIT---TKGGN---IPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIG-KPTERTKQVIYMLTENQKRDKLREIM-SRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDD 2259
            RE  +KW +  W +                 + ++    WR  +E+F+IT    K G    IPN    +++A      +   I KA +++PTPIQ QA PI LQ  D+IGVA+TG+GKT  +L+P    +   P L K ++N   P  ++L PTR+LA+Q+  E  K+    G+++V + GG +R+EQ+ +L+ G +I+IATPGRL D+  + ++ L   TY+++DE D+M+DM FE  + KIL  +     +PD                    RQTVM +AT   SV RLA+++L+ P  VY+G++        KQ I + TE +K   ++  + S +    +I+FV++K  AD L+  L   N+   S HG + Q  RE A+   K+ +  IL+AT+LA RG+D+ DV+ V N+D P+ I+EY+HR+GRTGRAG+ G++I+ +T++D
Sbjct:  177 REEGLKWQKTKWADLPPIKKNFYKESTATSAMSKVEADSWR--KENFNITWDDLKDGEKRPIPNPTCTFDDAFQCYPEVMENIKKAGFQKPTPIQSQAWPIVLQGIDLIGVAQTGTGKTLCYLMPGFIHLVLQPSL-KGQRNR--PGMLVLTPTRELALQVEGECCKYSYK-GLRSVCVYGGGNRDEQIEELKKGVDIIIATPGRLNDLQMSNFVNLKNITYLVLDEADKMLDMGFEPQIMKILLDV-----RPD--------------------RQTVMTSATWPHSVHRLAQSYLKEPMIVYVGTLDLVAVSSVKQNIIVTTEEEKWSHMQTFLQSMSSTDKVIVFVSRKAVADHLSSDLILGNISVESLHGDREQRDREKALENFKTGKVRILIATDLASRGLDVHDVTHVYNFDFPRNIEEYVHRIGRTGRAGRTGVSITTLTRND 595

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Human
Match: DDX41 (DEAD-box helicase 41 [Source:HGNC Symbol;Acc:HGNC:18674])

HSP 1 Score: 264.618 bits (675), Expect = 4.663e-75
Identity = 154/454 (33.92%), Postives = 248/454 (54.63%), Query Frame = 1

Query:  949 EAKRLKNISNREAKVKWDE---RPWTE-KHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQ---ILEETMKFGQ----PLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTK--DDSPLF 2271
            E + L ++      + +D+     WT  +++  M+       R+ + I  +G  IP  I+ ++E      +L  +  K  +  PTPIQ Q IP  L  RD+IG+A TGSGKT  F +P++ +  +  +     + E GPY +I+ P+R+LA Q   ILE   +  Q    PL ++    IGG S +EQ+  +R G  +++ATPGRL+D+L+ + + L+ C Y+ +DE DRMIDM FE D+R I SY                          +  RQT++F+ATM   ++  A++ L +P T+ +G  G  +    Q +  + E  K   L E + +   PP++IF  +K   D + + L    ++A++ HGGK QE R  A+ A +  +KD+LVAT++A +G+D   +  V+NYDMP+ I+ Y+HR+GRTGR+G  G+A +F+ K  D+S L 
Sbjct:  122 EGRALMSVKEMAKGITYDDPIKTSWTPPRYVLSMSEERHERVRKKYHILVEGDGIPPPIKSFKEMKFPAAILRGLKKKGIH-HPTPIQIQGIPTILSGRDMIGIAFTGSGKTLVFTLPVIMFCLEQEKRLPFSKRE-GPYGLIICPSRELARQTHGILEYYCRLLQEDSSPL-LRCALCIGGMSVKEQMETIRHGVHMMVATPGRLMDLLQKKMVSLDICRYLALDEADRMIDMGFEGDIRTIFSYF-------------------------KGQRQTLLFSATMPKKIQNFAKSALVKPVTINVGRAGAASLDVIQEVEYVKEEAKMVYLLECLQKT-PPPVLIFAEKKADVDAIHEYLLLKGVEAVAIHGGKDQEERTKAIEAFREGKKDVLVATDVASKGLDFPAIQHVINYDMPEEIENYVHRIGRTGRSGNTGIATTFINKACDESVLM 546

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Celegans
Match: ddx-23 (DEAD boX helicase homolog [Source:UniProtKB/TrEMBL;Acc:Q95QN2])

HSP 1 Score: 711.449 bits (1835), Expect = 0.000e+0
Identity = 354/570 (62.11%), Postives = 450/570 (78.95%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLYI 2406
            RYLG+++  K+R RRL+E+KFVF WD GEDTS DYN LY+ +HE QFFGRG + G D+NAQKK    FY +MM +RR+  +K+QE  RL+    +E KV  D+R W  K L EM++RDWRIFREDF+I+ KGG +P  +R WEEAG  D++   +  +  Y EPTPIQRQAIPIGLQNRD+IGVAETGSGKTAAFL+PLL WI  LP++++ E  +LGPYAII+ PTR+LA QI EET KFG+ LGIK V++IGG SRE+Q  +LR+G E+VIATPGRL+DVLENRY++LNQCTYVI+DE DRM+DM FE DV+K+L Y+P+   K DTD+ ++ E L++ F T +KYRQTVMFTATM  ++ERLAR +LRRPA V+IGS GKPTER +QV+YM+ E++KR KL E++   F PPIIIFVNQKKGAD+L+KGL K+  K    HGGKGQ+ REYA+ ALK    DILVAT++AGRGID+KDVSLVLNYDM K+I++Y HR+GRTGRAGK G AI+F+T DD+ +++DLK++L ESP+S  P EL +HPDAQ K G FTSKKR++ ETL++
Sbjct:  162 RYLGKQKEKKKRGRRLHEKKFVFDWDAGEDTSQDYNKLYQSRHEIQFFGRGSVAGTDVNAQKKEKNSFYQEMMENRRTVDEKEQEMHRLEKELKKEKKVAHDDRHWRMKELSEMSDRDWRIFREDFNISIKGGRVPRPLRNWEEAGFPDEVYQAV-KEIGYLEPTPIQRQAIPIGLQNRDVIGVAETGSGKTAAFLLPLLVWITSLPKMERQEHRDLGPYAIIMAPTRELAQQIEEETNKFGKLLGIKTVSVIGGASREDQGMKLRMGVEVVIATPGRLLDVLENRYLLLNQCTYVILDEADRMLDMGFEPDVQKVLEYMPDTNMKKDTDEFDNEEALMKGFSTREKYRQTVMFTATMSSAIERLARQYLRRPAVVHIGSAGKPTERVEQVVYMVPEDRKRKKLVEVLESQFQPPIIIFVNQKKGADMLSKGLTKLGFKPTVLHGGKGQDQREYALQALKEGTSDILVATDVAGRGIDVKDVSLVLNYDMAKSIEDYTHRIGRTGRAGKHGKAITFLTPDDTAVYFDLKQVLVESPVSSCPPELANHPDAQSKPGVFTSKKRQD-ETLFL 729

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Celegans
Match: F53H1.1 (pep chromosome:WBcel235:IV:1297400:1309644:-1 gene:WBGene00018776.1 transcript:F53H1.1b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F53H1.1)

HSP 1 Score: 279.256 bits (713), Expect = 1.987e-79
Identity = 155/410 (37.80%), Postives = 241/410 (58.78%), Query Frame = 1

Query: 1036 MTNRDWRIFREDF-SITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVL---ENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVP-PIIIFVNQKKGADVLAKGLEKMNLKAIS-FHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFV 2247
            MT  + + +RE+  SIT KG + P  I+ W + G++ K+++V+  K +Y +PT IQ QAIP  +  RD+IG+A+TGSGKT AFL+P+   I   P L++ +    GP A+IL PTR+LA+Q  +E  KF +PLG+K     GG    EQ+  L+ GAEIV+ TPGR++DVL     +   L + TY+++DE DRM D  FE  + K+++ +     +PD                    +QTV+F+AT    +E LAR  L +P  + +G          Q   +  E+QK  KL E++   +     I+FV++++ AD +   L +    +++  HGG  Q  R+ ++A  K+    +LVAT++A RG+D+K++ LV+NYD P   ++Y+HRVGRTGRAG++G A +FV
Sbjct:  277 MTKAEVKAYREELDSITVKGIDCPKPIKTWAQCGVNLKMMNVL-KKFEYSKPTSIQAQAIPSIMSGRDVIGIAKTGSGKTLAFLLPMFRHILDQPELEEGD----GPIAVILAPTRELAMQTYKEANKFAKPLGLKVACTYGGVGISEQIADLKRGAEIVVCTPGRMIDVLAANSGKVTNLRRVTYLVLDEADRMFDKGFEPQIMKVVNNI-----RPD--------------------KQTVLFSATFPRHMEALARKVLDKPVEILVGGKSVVCSDITQNAVICAEHQKFLKLLELLGMYYEEGSSIVFVDKQEKADDIVDQLMRTGYNSVAPLHGGIDQHDRDSSIADFKTGVIKVLVATSVAARGLDVKNLILVVNYDCPNHYEDYVHRVGRTGRAGRKGYAYTFV 656

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Celegans
Match: F53H1.1 (pep chromosome:WBcel235:IV:1297222:1309652:-1 gene:WBGene00018776.1 transcript:F53H1.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F53H1.1)

HSP 1 Score: 279.641 bits (714), Expect = 8.221e-78
Identity = 155/410 (37.80%), Postives = 241/410 (58.78%), Query Frame = 1

Query: 1036 MTNRDWRIFREDF-SITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVL---ENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVP-PIIIFVNQKKGADVLAKGLEKMNLKAIS-FHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFV 2247
            MT  + + +RE+  SIT KG + P  I+ W + G++ K+++V+  K +Y +PT IQ QAIP  +  RD+IG+A+TGSGKT AFL+P+   I   P L++ +    GP A+IL PTR+LA+Q  +E  KF +PLG+K     GG    EQ+  L+ GAEIV+ TPGR++DVL     +   L + TY+++DE DRM D  FE  + K+++ +     +PD                    +QTV+F+AT    +E LAR  L +P  + +G          Q   +  E+QK  KL E++   +     I+FV++++ AD +   L +    +++  HGG  Q  R+ ++A  K+    +LVAT++A RG+D+K++ LV+NYD P   ++Y+HRVGRTGRAG++G A +FV
Sbjct:  277 MTKAEVKAYREELDSITVKGIDCPKPIKTWAQCGVNLKMMNVL-KKFEYSKPTSIQAQAIPSIMSGRDVIGIAKTGSGKTLAFLLPMFRHILDQPELEEGD----GPIAVILAPTRELAMQTYKEANKFAKPLGLKVACTYGGVGISEQIADLKRGAEIVVCTPGRMIDVLAANSGKVTNLRRVTYLVLDEADRMFDKGFEPQIMKVVNNI-----RPD--------------------KQTVLFSATFPRHMEALARKVLDKPVEILVGGKSVVCSDITQNAVICAEHQKFLKLLELLGMYYEEGSSIVFVDKQEKADDIVDQLMRTGYNSVAPLHGGIDQHDRDSSIADFKTGVIKVLVATSVAARGLDVKNLILVVNYDCPNHYEDYVHRVGRTGRAGRKGYAYTFV 656

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Celegans
Match: vbh-1 (Vasa-and Belle-like Helicase [Source:UniProtKB/TrEMBL;Acc:Q65XX1])

HSP 1 Score: 267.7 bits (683), Expect = 1.821e-76
Identity = 159/433 (36.72%), Postives = 243/433 (56.12%), Query Frame = 1

Query:  994 KWDERP--WTEKHLDEMTNRDWRIFR-EDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWI-EKLPRLQKMEQNELG-----PYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGG-NSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSY-LPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPAT-VYIGSIGKPTERTKQVIYMLTENQKRDKLREIM----SRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISF 2244
            +W   P  ++E +L   T+      + E+  +   G ++P +I  + EAG    +++ + N++ Y +PTP+Q+ +IP  L NRD++  A+TGSGKTAAFL+P++  I    P + K      G     P A++L PTR+LAIQI +E  KF     I+   + GG  +  +QV +LR G  I+IATPGRL+D++E  +I L  C Y+++DE DRM+DM FE  +RKI+   +P  T                        R T MF+AT    ++ LA+ FL+     + +G +G  +E  +Q +  + E +KR  L EI+    S N V   ++FV  K+GA+ LA  L +  ++++S HG   Q  RE  +   +S +  ILVAT +A RG+DI +V  V+NYD+P   DEY+HR+GRTGR G  G+A SF
Sbjct:   77 QWGGAPAEYSESNLFHRTDSGINFDKYENIPVEVSGDSVPAAIEHFNEAGFGPAVMENV-NRSGYSKPTPVQKHSIPTLLANRDLMSCAQTGSGKTAAFLLPIIQHILAGGPDMVKPPAFTNGRRTYYPCALVLSPTRELAIQIHKEATKFSYKSNIQTAILYGGRENYRDQVNRLRAGTHILIATPGRLIDIIEQGFIGLAGCRYLVLDEADRMLDMGFEPQIRKIVGQGMPPKTA-----------------------RTTAMFSATFPKEIQVLAKDFLKDNYIFLAVGRVGSTSENIEQRLLWVNEMEKRSNLMEILMNEHSENLV---LVFVETKRGANELAYFLNRQQIRSVSIHGDLKQIERERNLELFRSGQCPILVATAVAARGLDIPNVRHVINYDLPGDSDEYVHRIGRTGRCGNLGIATSF 482

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Query:  994 KWDERP--WTEKHLDEMTNRDWRIFR-EDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWI-EKLPRLQKMEQNELG-----PYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGG-NSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSY-LPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPAT-VYIGSIGKPTERTKQVIYMLTENQKRDKLREIM----SRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISF 2244
            +W   P  ++E +L   T+      + E+  +   G ++P +I  + EAG    +++ + N++ Y +PTP+Q+ +IP  L NRD++  A+TGSGKTAAFL+P++  I    P + K      G     P A++L PTR+LAIQI +E  KF     I+   + GG  +  +QV +LR G  I+IATPGRL+D++E  +I L  C Y+++DE DRM+DM FE  +RKI+   +P  T                        R T MF+AT    ++ LA+ FL+     + +G +G  +E  +Q +  + E +KR  L EI+    S N V   ++FV  K+GA+ LA  L +  ++++S HG   Q  RE  +   +S +  ILVAT +A RG+DI +V  V+NYD+P   DEY+HR+GRTGR G  G+A SF
Sbjct:   77 QWGGAPAEYSESNLFHRTDSGINFDKYENIPVEVSGDSVPAAIEHFNEAGFGPAVMENV-NRSGYSKPTPVQKHSIPTLLANRDLMSCAQTGSGKTAAFLLPIIQHILAGGPDMVKPPAFTNGRRTYYPCALVLSPTRELAIQIHKEATKFSYKSNIQTAILYGGRENYRDQVNRLRAGTHILIATPGRLIDIIEQGFIGLAGCRYLVLDEADRMLDMGFEPQIRKIVGQGMPPKTA-----------------------RTTAMFSATFPKEIQVLAKDFLKDNYIFLAVGRVGSTSENIEQRLLWVNEMEKRSNLMEILMNEHSENLV---LVFVETKRGANELAYFLNRQQIRSVSIHGDLKQIERERNLELFRSGQCPILVATAVAARGLDIPNVRHVINYDLPGDSDEYVHRIGRTGRCGNLGIATSF 482

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Fly
Match: CG10333 (gene:FBgn0032690 transcript:FBtr0081072)

HSP 1 Score: 786.178 bits (2029), Expect = 0.000e+0
Identity = 382/565 (67.61%), Postives = 462/565 (81.77%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNE 2391
            RYLG  +  +R +R  N+RKFVF WD GEDTS DYN LYK++H  QFFGRG++ GIDI  QK+   +FY  ++  RR++ +K+QE  RLK +  +E K KWD+R W+EK  DEMT RDWRIFRED+++T KGG IPN IR W E+G   +++D II+K  YKEPTPIQRQAIPIGLQNRDIIGVAETGSGKT AFLIPLLSWI+ LP+++++E  + GPYAII+ PTR+LA QI EET KFGQPLGI+ V ++GG SREEQ F+LRLG EIVIATPGRL+DVLENRY+VLNQCTY+++DE DRMIDM FE DV+KIL Y+P    KPDT++AED  KL++NF+T +KYRQTVMFTATM P+VERLART+LRRPATVYIGS+GKPTERT+Q++YM+ EN KR KL EI+SR   PP+IIFVNQKKGADVLAKGLEK+   + + HGGKGQE REYA+AALKS  KDILVAT++AGRGIDIKDVSLV+NYDM KTI++Y HR+GRTGRAGK G AISFVTKDDS LFYDLK+ +  SP+S  P EL++HP+AQ K GT  +KKRR E
Sbjct:  256 RYLGIIKKKRRVRRL-NDRKFVFDWDAGEDTSIDYNNLYKERHHVQFFGRGNVAGIDIKEQKRTQSKFYGDLLEKRRTEAEKEQEKVRLKKMKRKEDKQKWDDRHWSEKENDEMTERDWRIFREDYNVTIKGGRIPNPIRSWNESGFPKEIID-IIDKVGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTLAFLIPLLSWIQSLPKIERLEDVDQGPYAIIMAPTRELAQQIEEETTKFGQPLGIRTVVVVGGLSREEQGFRLRLGCEIVIATPGRLIDVLENRYLVLNQCTYIVLDEADRMIDMGFEPDVQKILEYMPVTNLKPDTEEAEDETKLMENFYTKKKYRQTVMFTATMPPAVERLARTYLRRPATVYIGSVGKPTERTEQIVYMMGENDKRKKLMEILSRKIDPPVIIFVNQKKGADVLAKGLEKLGYNSCTLHGGKGQEQREYALAALKSGAKDILVATDVAGRGIDIKDVSLVINYDMAKTIEDYTHRIGRTGRAGKTGCAISFVTKDDSALFYDLKQCVSASPVSTCPPELMNHPEAQHKPGTVVTKKRREE 818

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Fly
Match: vas (gene:FBgn0283442 transcript:FBtr0445187)

HSP 1 Score: 278.87 bits (712), Expect = 5.946e-80
Identity = 154/390 (39.49%), Postives = 224/390 (57.44%), Query Frame = 1

Query: 1078 ITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELG-PYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISF 2244
            +   G ++P  I+ +  A L D ++D + NK+ YK PTPIQ+ +IP+    RD++  A+TGSGKTAAFL+P+LS +     L+   + ELG P  +I+ PTR+LAIQI  E  KF     +K   + GG S   Q   +  G  +VIATPGRL+D ++  +I      +V++DE DRM+DM F  D+R+I++++   T +P+                     QT+MF+AT    ++R+A  FL+    V IG +G      KQ IY + +  KR KL EI+S       I+FV  K+GAD LA  L +      S HG + Q  RE A+   K+    +L+AT++A RG+DIK++  V+NYDMP  ID+Y+HR+GRTGR G  G A SF
Sbjct:  233 VKVTGSDVPQPIQHFTSADLRDIIIDNV-NKSGYKIPTPIQKCSIPVISSGRDLMACAQTGSGKTAAFLLPILSKL-----LEDPHELELGRPQVVIVSPTRELAIQIFNEARKFAFESYLKIGIVYGGTSFRHQNECITRGCHVVIATPGRLLDFVDRTFITFEDTRFVVLDEADRMLDMGFSEDMRRIMTHV---TMRPE--------------------HQTLMFSATFPEEIQRMAGEFLKNYVFVAIGIVGGACSDVKQTIYEVNKYAKRSKLIEILSEQ-ADGTIVFVETKRGADFLASFLSEKEFPTTSIHGDRLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIGRTGRVGNNGRATSF 592

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Fly
Match: vas (gene:FBgn0283442 transcript:FBtr0445185)

HSP 1 Score: 278.87 bits (712), Expect = 5.946e-80
Identity = 154/390 (39.49%), Postives = 224/390 (57.44%), Query Frame = 1

Query: 1078 ITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELG-PYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISF 2244
            +   G ++P  I+ +  A L D ++D + NK+ YK PTPIQ+ +IP+    RD++  A+TGSGKTAAFL+P+LS +     L+   + ELG P  +I+ PTR+LAIQI  E  KF     +K   + GG S   Q   +  G  +VIATPGRL+D ++  +I      +V++DE DRM+DM F  D+R+I++++   T +P+                     QT+MF+AT    ++R+A  FL+    V IG +G      KQ IY + +  KR KL EI+S       I+FV  K+GAD LA  L +      S HG + Q  RE A+   K+    +L+AT++A RG+DIK++  V+NYDMP  ID+Y+HR+GRTGR G  G A SF
Sbjct:  233 VKVTGSDVPQPIQHFTSADLRDIIIDNV-NKSGYKIPTPIQKCSIPVISSGRDLMACAQTGSGKTAAFLLPILSKL-----LEDPHELELGRPQVVIVSPTRELAIQIFNEARKFAFESYLKIGIVYGGTSFRHQNECITRGCHVVIATPGRLLDFVDRTFITFEDTRFVVLDEADRMLDMGFSEDMRRIMTHV---TMRPE--------------------HQTLMFSATFPEEIQRMAGEFLKNYVFVAIGIVGGACSDVKQTIYEVNKYAKRSKLIEILSEQ-ADGTIVFVETKRGADFLASFLSEKEFPTTSIHGDRLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIGRTGRVGNNGRATSF 592

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Fly
Match: vas (gene:FBgn0283442 transcript:FBtr0445186)

HSP 1 Score: 278.87 bits (712), Expect = 5.946e-80
Identity = 154/390 (39.49%), Postives = 224/390 (57.44%), Query Frame = 1

Query: 1078 ITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELG-PYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISF 2244
            +   G ++P  I+ +  A L D ++D + NK+ YK PTPIQ+ +IP+    RD++  A+TGSGKTAAFL+P+LS +     L+   + ELG P  +I+ PTR+LAIQI  E  KF     +K   + GG S   Q   +  G  +VIATPGRL+D ++  +I      +V++DE DRM+DM F  D+R+I++++   T +P+                     QT+MF+AT    ++R+A  FL+    V IG +G      KQ IY + +  KR KL EI+S       I+FV  K+GAD LA  L +      S HG + Q  RE A+   K+    +L+AT++A RG+DIK++  V+NYDMP  ID+Y+HR+GRTGR G  G A SF
Sbjct:  233 VKVTGSDVPQPIQHFTSADLRDIIIDNV-NKSGYKIPTPIQKCSIPVISSGRDLMACAQTGSGKTAAFLLPILSKL-----LEDPHELELGRPQVVIVSPTRELAIQIFNEARKFAFESYLKIGIVYGGTSFRHQNECITRGCHVVIATPGRLLDFVDRTFITFEDTRFVVLDEADRMLDMGFSEDMRRIMTHV---TMRPE--------------------HQTLMFSATFPEEIQRMAGEFLKNYVFVAIGIVGGACSDVKQTIYEVNKYAKRSKLIEILSEQ-ADGTIVFVETKRGADFLASFLSEKEFPTTSIHGDRLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIGRTGRVGNNGRATSF 592

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Fly
Match: CG6227 (gene:FBgn0030631 transcript:FBtr0074011)

HSP 1 Score: 289.271 bits (739), Expect = 1.076e-79
Identity = 159/418 (38.04%), Postives = 245/418 (58.61%), Query Frame = 1

Query: 1024 HLDEMTNRDWRIFREDF-SITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVL---ENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMS-RNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDS 2262
             L  MT  D   +R D   I  KG   P  I+ W + G+S K ++V+  +  +++PTPIQ QAIP  +  RD+IG+A+TGSGKT AF++P+   I   P ++  +    G  AII+ PTR+L +QI ++  KF + LG++ V + GG    EQ+ +L+ GAEI++ TPGR++D+L     R   L + TYV++DE DRM DM FE  V +I+  +     +PD                    RQTVMF+AT    +E LAR  L++P  V +G      +  +Q + +L ++ K  KL E++        II+FV++++ AD+L + L K +   +S HGG  Q  R+  +   KS +  +L+AT++A RG+D+KD+ LV+NYD+P   ++Y+HR GRTGRAGK+G A +F+T + S
Sbjct:  479 ELTRMTAADVEKYRSDLEGIQVKGKGCPKPIKTWAQCGVSKKEMEVL-RRLGFEKPTPIQCQAIPAIMSGRDLIGIAKTGSGKTLAFILPMFRHILDQPSMEDGD----GAIAIIMAPTRELCMQIGKDIRKFSKSLGLRPVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIIDNV-----RPD--------------------RQTVMFSATFPRQMEALARRILKKPIEVIVGGRSVVCKEVEQHVVILNDDAKFFKLLELLGIYQEAGSIIVFVDKQENADILLRDLMKASYPCMSLHGGIDQFDRDSTIIDFKSGKVRLLIATSVAARGLDVKDLILVVNYDVPNHYEDYVHRCGRTGRAGKKGSAYTFITPEQS 866

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Zebrafish
Match: ddx23 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 [Source:ZFIN;Acc:ZDB-GENE-030131-6215])

HSP 1 Score: 749.969 bits (1935), Expect = 0.000e+0
Identity = 367/570 (64.39%), Postives = 459/570 (80.53%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            RYLG  +  +R +   N+RKFVF WD  EDTS DYNP+YK+KH+   +GRG I GID+  QK+   +FY  +M  RR++ +K+QE +RLK +  +EAK +WD+R W++K LDEMT+RDWRIFRED+SITTKGG IPN IR W+E  L   +L+VI  K  YK+PTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL WI  LP++ ++E ++ GPYAIIL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G EIVIATPGRL+DVLENRY+VL++CTYV++DE DRMIDM FE DV+KIL Y+P   +KPDTDDAED EK++QNF +   KYRQTVMFTATM P+VERLAR++LRRPA VYIGS GKP ER +Q + +++E +KR KL E+++  F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI+DVS+VLNYDM K I++YIHR+GRTGRAGK G+A++F+TK+DS +FYDLK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:  240 RYLGGMKKRRRTRHL-NDRKFVFEWDASEDTSIDYNPIYKEKHQVHLYGRGFIAGIDLKQQKRDQSRFYGDLMEKRRTNEEKEQEEQRLKKVRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRNWKEYSLPPHILEVI-EKCGYKDPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEDSDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEYIPVTNQKPDTDDAEDPEKMMQNFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVILMSEGEKRKKLLEVLASGFEPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVLNYDMAKNIEDYIHRIGRTGRAGKSGVAMTFLTKEDSSVFYDLKQAILESPVSTCPPELANHPDAQHKPGTILTKKRR-EETIF 806

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Zebrafish
Match: ddx23 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 [Source:ZFIN;Acc:ZDB-GENE-030131-6215])

HSP 1 Score: 716.072 bits (1847), Expect = 0.000e+0
Identity = 360/571 (63.05%), Postives = 452/571 (79.16%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWD-EGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            RYLG  +  +R +   N+RKFVF WD      + D + L  KKH+   +GRG I GID+  QK+   +FY  +M  RR++ +K+QE +RLK +  +EAK +WD+R W++K LDEMT+RDWRIFRED+SITTKGG IPN IR W+E  L   +L+VI  K  YK+PTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL WI  LP++ ++E ++ GPYAIIL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G EIVIATPGRL+DVLENRY+VL++CTYV++DE DRMIDM FE DV+KIL Y+P   +KPDTDDAED EK++QNF +   KYRQTVMFTATM P+VERLAR++LRRPA VYIGS GKP ER +Q + +++E +KR KL E+++  F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI+DVS+VLNYDM K I++YIHR+GRTGRAGK G+A++F+TK+DS +FYDLK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:  181 RYLGGMKKRRRTRHL-NDRKFVFEWDYRFIYIAIDLHCL--KKHQVHLYGRGFIAGIDLKQQKRDQSRFYGDLMEKRRTNEEKEQEEQRLKKVRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRNWKEYSLPPHILEVI-EKCGYKDPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEDSDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEYIPVTNQKPDTDDAEDPEKMMQNFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVILMSEGEKRKKLLEVLASGFEPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVLNYDMAKNIEDYIHRIGRTGRAGKSGVAMTFLTKEDSSVFYDLKQAILESPVSTCPPELANHPDAQHKPGTILTKKRR-EETIF 746

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Zebrafish
Match: CR936486.1 (pep chromosome:GRCz11:CHR_ALT_CTG23_2_1:14207403:14214672:-1 gene:ENSDARG00000110234.1 transcript:ENSDART00000186263.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR936486.1)

HSP 1 Score: 409.838 bits (1052), Expect = 1.110e-131
Identity = 202/304 (66.45%), Postives = 252/304 (82.89%), Query Frame = 1

Query: 1495 LGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            +G EIVIATPGRL+DVLENRY+VL++CTYV++DE DRMIDM FE DV+KIL Y+P   +KPDTDDAED EK++QNF +   KYRQTVMFTATM P+VERLAR++LRRPA VYIGS GKP ER +Q + +++E +KR KL E+++  F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI+DVS+VLNYDM K I++YIHR+GRTGRAGK G+A++F+TK+DS +FYDLK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:    1 MGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEYIPVTNQKPDTDDAEDPEKMMQNFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVILMSEGEKRKKLLEVLASGFEPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVLNYDMAKNIEDYIHRIGRTGRAGKSGVAMTFLTKEDSSVFYDLKQAILESPVSTCPPELANHPDAQHKPGTILTKKRR-EETIF 303

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Zebrafish
Match: ddx46 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 [Source:NCBI gene;Acc:321948])

HSP 1 Score: 278.485 bits (711), Expect = 5.674e-77
Identity = 155/396 (39.14%), Postives = 231/396 (58.33%), Query Frame = 1

Query: 1078 ITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVL---ENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSR-NFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTK 2253
            I+ KG   P  I+ W + G+S K+L+ +  K  Y++PTPIQ QAIP  +  RD+IG+A+TGSGKT AFL+P+   I     + + E    GP A+I+ PTR+LA+QI +E  KF + L ++ V + GG    EQ+ +L+ GAEI++ TPGR++D+L     R   L + TYV++DE DRM DM FE  V +I+  +     +PD                    RQTVMF+AT   ++E LAR  L +P  V +G         +Q + ++ E +K  KL EI+        +IIFV++++ AD L K L K +   +S HGG  Q  R+  +   K+    +LVAT++A RG+D+K + LV+NY  P   ++Y+HR GRTGRAG +G A +F+T+
Sbjct:  328 ISVKGKGCPKPIKTWVQCGISMKVLNAL-KKHNYEKPTPIQAQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHILDQRPVGEAE----GPLAVIMTPTRELALQITKECKKFSKSLALRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLGANNGRVTNLRRVTYVVIDEADRMFDMGFEPQVMRIVDNV-----RPD--------------------RQTVMFSATFPRTMEALARRILSKPVEVQVGGRSVVCSDVEQHVIVIEEEKKFLKLLEILGHYQEKGSVIIFVDKQEHADGLLKDLMKASYPCMSLHGGIDQYDRDSIINDFKNGACRLLVATSVAARGLDVKQLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITE 693

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Zebrafish
Match: DDX17 (si:dkey-156n14.5 [Source:ZFIN;Acc:ZDB-GENE-030131-18])

HSP 1 Score: 268.855 bits (686), Expect = 2.948e-76
Identity = 153/445 (34.38%), Postives = 241/445 (54.16%), Query Frame = 1

Query:  955 KRLKNISNREAKVKWD--ERPWTEKH-------LDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIG-KPTERTKQVIYMLTENQKRDKLREIMSRNFVP---PIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVT 2250
            K+  N  +R  K KWD  + P  EK+       +  M+  D   +R    IT +G   P  +  + +A     ++DV++ +  +KEPT IQ Q  P+ L  RD++G+A+TGSGKT A+L+P +  I   P L++ +    GP  ++L PTR+LA Q+ +    +G+   IK+  + GG  +  Q+  L  G EI IATPGRL+D LE     L +CTY+++DE DRM+DM FE  +RKI+  +     +PD                    RQT+M++AT    V +LA  FL+    + IG++         Q++ +  EN+K +KL ++M           IIFV  KK  D L + + +    A+  HG K Q  R++ +   +S +  IL+AT++A RG+D++DV  V+NYD P + ++Y+HR+GRT R+  +G A +F T
Sbjct:   33 KKFGNPGDRLRKKKWDLDQLPKFEKNFYNENPEVHHMSQYDVEEYRRKREITVRGSGCPKPVTNFHQAQFPQYVMDVLLQQ-NFKEPTAIQAQGFPLALSGRDMVGIAQTGSGKTLAYLLPAIVHINHQPYLERGD----GPICLVLAPTRELAQQVQQVAFDYGKSSRIKSTCVYGGAPKGPQIRDLERGVEICIATPGRLIDFLEVGKTNLRRCTYLVLDEADRMLDMGFEPQIRKIVDQI-----RPD--------------------RQTLMWSATWPKEVRQLAEDFLQDYVQINIGALELSANHNILQIVDVCMENEKDNKLIQLMEEIMAEKENKTIIFVETKKRCDELTRRMRRDGWPAMCIHGDKSQPERDWVLTEFRSGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYVHRIGRTARSTNKGTAYTFFT 447

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Xenopus
Match: ankrd9 (ankyrin repeat domain 9 [Source:Xenbase;Acc:XB-GENE-955835])

HSP 1 Score: 744.577 bits (1921), Expect = 0.000e+0
Identity = 364/570 (63.86%), Postives = 457/570 (80.18%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            RYLG  +  +R +   N+RKFVF WD  EDTS DYNPLYK++H+ Q  GRG I GID+  QK+   +FY  +M  RR+  +K+QE  RL+ +  +EAK +WD+R W++K LDEMT+RDWRIFRED+SITTKGG IPN IR W ++ L   +L+VI +K  YKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL WI  LP++ ++E+++ GPYAIIL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G EIVIATPGRL+DVLENRY+VL++CTYV++DE DRMIDM FE DV+KIL ++P   +KPDTD+AED EK+  NF +   KYRQTVMFTATM P+VERLAR++LRRPA VYIGS GKP ER +Q +++++E +KR KL  I+ + F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI+DVS+V+NYDM K I++YIHR+GRTGRAGK G+AI+F+TK+DS +FYDLK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:  241 RYLGGLKKRRRTRHL-NDRKFVFEWDASEDTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEVRLRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWVDSSLPPHILEVI-DKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEHMPVTNQKPDTDEAEDPEKMTANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSEAEKRKKLLAILEKGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSSVFYDLKQAILESPVSSCPPELANHPDAQHKPGTILTKKRR-EETIF 807

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Xenopus
Match: ankrd9 (ankyrin repeat domain 9 [Source:Xenbase;Acc:XB-GENE-955835])

HSP 1 Score: 744.191 bits (1920), Expect = 0.000e+0
Identity = 364/570 (63.86%), Postives = 457/570 (80.18%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            RYLG  +  +R +   N+RKFVF WD  EDTS DYNPLYK++H+ Q  GRG I GID+  QK+   +FY  +M  RR+  +K+QE  RL+ +  +EAK +WD+R W++K LDEMT+RDWRIFRED+SITTKGG IPN IR W ++ L   +L+VI +K  YKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL WI  LP++ ++E+++ GPYAIIL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G EIVIATPGRL+DVLENRY+VL++CTYV++DE DRMIDM FE DV+KIL ++P   +KPDTD+AED EK+  NF +   KYRQTVMFTATM P+VERLAR++LRRPA VYIGS GKP ER +Q +++++E +KR KL  I+ + F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI+DVS+V+NYDM K I++YIHR+GRTGRAGK G+AI+F+TK+DS +FYDLK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:  217 RYLGGLKKRRRTRHL-NDRKFVFEWDASEDTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEVRLRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWVDSSLPPHILEVI-DKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEHMPVTNQKPDTDEAEDPEKMTANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSEAEKRKKLLAILEKGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSSVFYDLKQAILESPVSSCPPELANHPDAQHKPGTILTKKRR-EETIF 783

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Xenopus
Match: ankrd9 (ankyrin repeat domain 9 [Source:Xenbase;Acc:XB-GENE-955835])

HSP 1 Score: 741.88 bits (1914), Expect = 0.000e+0
Identity = 364/570 (63.86%), Postives = 457/570 (80.18%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            RYLG  +  +R +   N+RKFVF WD  EDTS DYNPLYK++H+ Q  GRG I GID+  QK+   +FY  +M  RR+  +K+QE  RL+ +  +EAK +WD+R W++K LDEMT+RDWRIFRED+SITTKGG IPN IR W ++ L   +L+VI +K  YKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL WI  LP++ ++E+++ GPYAIIL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G EIVIATPGRL+DVLENRY+VL++CTYV++DE DRMIDM FE DV+KIL ++P   +KPDTD+AED EK+  NF +   KYRQTVMFTATM P+VERLAR++LRRPA VYIGS GKP ER +Q +++++E +KR KL  I+ + F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI+DVS+V+NYDM K I++YIHR+GRTGRAGK G+AI+F+TK+DS +FYDLK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:  161 RYLGGLKKRRRTRHL-NDRKFVFEWDASEDTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEVRLRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWVDSSLPPHILEVI-DKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEHMPVTNQKPDTDEAEDPEKMTANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSEAEKRKKLLAILEKGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSSVFYDLKQAILESPVSSCPPELANHPDAQHKPGTILTKKRR-EETIF 727

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Xenopus
Match: ankrd9 (ankyrin repeat domain 9 [Source:Xenbase;Acc:XB-GENE-955835])

HSP 1 Score: 740.725 bits (1911), Expect = 0.000e+0
Identity = 364/570 (63.86%), Postives = 457/570 (80.18%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            RYLG  +  +R +   N+RKFVF WD  EDTS DYNPLYK++H+ Q  GRG I GID+  QK+   +FY  +M  RR+  +K+QE  RL+ +  +EAK +WD+R W++K LDEMT+RDWRIFRED+SITTKGG IPN IR W ++ L   +L+VI +K  YKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL WI  LP++ ++E+++ GPYAIIL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G EIVIATPGRL+DVLENRY+VL++CTYV++DE DRMIDM FE DV+KIL ++P   +KPDTD+AED EK+  NF +   KYRQTVMFTATM P+VERLAR++LRRPA VYIGS GKP ER +Q +++++E +KR KL  I+ + F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI+DVS+V+NYDM K I++YIHR+GRTGRAGK G+AI+F+TK+DS +FYDLK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:  101 RYLGGLKKRRRTRHL-NDRKFVFEWDASEDTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEVRLRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWVDSSLPPHILEVI-DKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEHMPVTNQKPDTDEAEDPEKMTANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSEAEKRKKLLAILEKGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSSVFYDLKQAILESPVSSCPPELANHPDAQHKPGTILTKKRR-EETIF 667

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Xenopus
Match: ankrd9 (ankyrin repeat domain 9 [Source:Xenbase;Acc:XB-GENE-955835])

HSP 1 Score: 714.916 bits (1844), Expect = 0.000e+0
Identity = 356/570 (62.46%), Postives = 449/570 (78.77%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            RYLG  +  +R +   N+RKFVF WD      A     YK++H+ Q  GRG I GID+  QK+   +FY  +M  RR+  +K+QE  RL+ +  +EAK +WD+R W++K LDEMT+RDWRIFRED+SITTKGG IPN IR W ++ L   +L+VI +K  YKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL WI  LP++ ++E+++ GPYAIIL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G EIVIATPGRL+DVLENRY+VL++CTYV++DE DRMIDM FE DV+KIL ++P   +KPDTD+AED EK+  NF +   KYRQTVMFTATM P+VERLAR++LRRPA VYIGS GKP ER +Q +++++E +KR KL  I+ + F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI+DVS+V+NYDM K I++YIHR+GRTGRAGK G+AI+F+TK+DS +FYDLK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:   79 RYLGGLKKRRRTRHL-NDRKFVFEWD------AKCFSRYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEVRLRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWVDSSLPPHILEVI-DKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEHMPVTNQKPDTDEAEDPEKMTANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSEAEKRKKLLAILEKGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSSVFYDLKQAILESPVSSCPPELANHPDAQHKPGTILTKKRR-EETIF 639

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Mouse
Match: Ddx23 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 [Source:MGI Symbol;Acc:MGI:1921601])

HSP 1 Score: 747.658 bits (1929), Expect = 0.000e+0
Identity = 364/570 (63.86%), Postives = 461/570 (80.88%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            RYLG  +  +R +   N+RKFVF WD  EDTS DYNPLYK++H+ Q  GRG I GID+  QK+   +FY  +M  RR+  +K+QE  RL+ +  +EAK +WD+R W++K LDEMT+RDWRIFRED+SITTKGG IPN IR W+++ L   +L+VI +K  YKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL WI  LP++ ++E+++ GPYAIIL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G EIVIATPGRL+DVLENRY+VL++CTYV++DE DRMIDM FE DV+KIL ++P + +KPDTD+AED EK+L NF +   KYRQTVMFTATM P+VERLAR++LRRPA VYIGS GKP ER +Q +++++E++KR KL  I+ + F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI+DVS+V+NYDM K I++YIHR+GRTGRAGK G+AI+F+TK+DS +FY+LK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:  252 RYLGGIKKRRRTRHL-NDRKFVFEWDASEDTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEARLRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWKDSSLPPHILEVI-DKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEHMPVSNQKPDTDEAEDPEKMLANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSESEKRKKLLAILEQGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSAVFYELKQAILESPVSSCPPELANHPDAQHKPGTILTKKRR-EETIF 818

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Mouse
Match: Ddx43 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 43 [Source:MGI Symbol;Acc:MGI:3642857])

HSP 1 Score: 273.478 bits (698), Expect = 5.018e-78
Identity = 166/451 (36.81%), Postives = 262/451 (58.09%), Query Frame = 1

Query:  979 REAKVKWDERPW---------------TEKHLDEMTNRDWRIFREDFSITT---KGGN---IPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERT-KQVIYMLTENQKRDKLREIMSRNFVPP--IIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDD 2259
            RE  +KW+++ W               T   + ++   +WR  +E+F+IT    K G    IPN I  +E+A  S   +   I +A +++PTPIQ QA PI LQ  D+IGVA+TG+GKT ++L+P    ++  P L + ++N  GP  ++L PTR+LA+Q+  E  K+     +K+V + GG  R+ Q+  +  G +I+IATPGRL D+  N ++ L   TY+++DE D+M+DM FE  + KIL  +     +PD                    RQT+M +AT   +V RLA+++L+ P  VY+G++      T KQ I + TE +KR  ++  +  N  P   +I+FV++K  AD L+  L   ++   S HG + Q  RE A+   K+ +  IL+AT+LA RG+D+ D++ V NYD P+ I+EY+HRVGRTGRAG+ G++I+ +T++D
Sbjct:  175 REDALKWEKKKWADLPPIKKNFYIESATTSSMSQVQIDNWR--KENFNITCDDLKDGEKRPIPNPICKFEDAFQSYPEVMENIKRAGFQKPTPIQSQAWPIVLQGIDLIGVAQTGTGKTLSYLMPGFIHLDSQP-LAREQRN--GPGMLVLTPTRELALQVEAECSKYSYG-DLKSVCVYGGGDRDGQIQDVSKGVDIIIATPGRLNDLQMNNFVNLKSVTYLVLDEADKMLDMGFEPQIMKILLDV-----RPD--------------------RQTIMTSATWPYAVRRLAQSYLKEPMIVYVGTLDLVAVSTVKQNIIITTEEEKRTHIQTFLE-NMSPKDKVIVFVSRKAVADHLSSDLILRHISVESLHGNREQSDREKALENFKTGKVRILIATDLASRGLDVHDITHVYNYDFPRNIEEYVHRVGRTGRAGRTGMSITLITRND 593

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Mouse
Match: Ddx46 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 [Source:MGI Symbol;Acc:MGI:1920895])

HSP 1 Score: 280.026 bits (715), Expect = 2.181e-77
Identity = 161/420 (38.33%), Postives = 244/420 (58.10%), Query Frame = 1

Query: 1024 HLDEMTNRDWRIFREDF-SITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVL---ENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKP---TERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDS 2262
             L +M+  +  +FR +   IT KG   P  I+ W + G+S K+L+ +  K  Y++PTPIQ QAIP  +  RD+IG+A+TGSGKT AFL+P+   I     L++ E    GP A+I+ PTR+LA+QI +E  KF + LG++ V + GG    EQ+ +L+ GAEI++ TPGR++D+L     R   L + TYV++DE DRM DM FE  V +I+  +     +PD                    RQTVMF+AT   ++E LAR  L +P  V +G  G+    ++  +QVI +  E +    L  +        +IIFV++++ AD L K L + +   +S HGG  Q  R+  +   K+    +LVAT++A RG+D+K + LV+NY  P   ++Y+HR GRTGRAG +G A +F+T+D +
Sbjct:  341 ELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSL-KKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGE----GPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNV-----RPD--------------------RQTVMFSATFPRAMEALARRILSKPIEVQVG--GRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQA 728

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Query: 1024 HLDEMTNRDWRIFREDF-SITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVL---ENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKP---TERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDS 2262
             L +M+  +  +FR +   IT KG   P  I+ W + G+S K+L+ +  K  Y++PTPIQ QAIP  +  RD+IG+A+TGSGKT AFL+P+   I     L++ E    GP A+I+ PTR+LA+QI +E  KF + LG++ V + GG    EQ+ +L+ GAEI++ TPGR++D+L     R   L + TYV++DE DRM DM FE  V +I+  +     +PD                    RQTVMF+AT   ++E LAR  L +P  V +G  G+    ++  +QVI +  E +    L  +        +IIFV++++ AD L K L + +   +S HGG  Q  R+  +   K+    +LVAT++A RG+D+K + LV+NY  P   ++Y+HR GRTGRAG +G A +F+T+D +
Sbjct:  341 ELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSL-KKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGE----GPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNV-----RPD--------------------RQTVMFSATFPRAMEALARRILSKPIEVQVG--GRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQA 728

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Mouse
Match: Ddx41 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 41 [Source:MGI Symbol;Acc:MGI:1920185])

HSP 1 Score: 263.077 bits (671), Expect = 8.126e-75
Identity = 154/454 (33.92%), Postives = 247/454 (54.41%), Query Frame = 1

Query:  949 EAKRLKNISNREAKVKWDE---RPWTE-KHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQ---ILEETMKFGQ----PLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTK--DDSPLF 2271
            E + L ++      + +D+     WT  +++  M+       R+ + I  +G  IP  I+ ++E      +L  +  K     PTPIQ Q IP  L  RD+IG+A TGSGKT  F +P++ +  +  +     + E GPY +I+ P+R+LA Q   ILE   +  Q    PL ++    IGG S +EQ+  +R G  +++ATPGRL+D+L+ + + L+ C Y+ +DE DRMIDM FE D+R I SY                          +  RQT++F+ATM   ++  A++ L +P T+ +G  G  +    Q +  + E  K   L E + +   PP++IF  +K   D + + L    ++A++ HGGK QE R  A+ A +  +KD+LVAT++A +G+D   +  V+NYDMP+ I+ Y+HR+GRTGR+G  G+A +F+ K  D+S L 
Sbjct:  122 EGRALMSVKEMAKGITYDDPIKTSWTPPRYVLSMSEERHERVRKKYHILVEGDGIPPPIKSFKEMKFPAAILRGL-KKKGILHPTPIQIQGIPTILSGRDMIGIAFTGSGKTLVFTLPVIMFCLEQEKRLPFSKRE-GPYGLIICPSRELARQTHGILEYYCRLLQEDSSPL-LRCALCIGGMSVKEQMETIRHGVHMMVATPGRLMDLLQKKMVSLDICRYLALDEADRMIDMGFEGDIRTIFSYF-------------------------KGQRQTLLFSATMPKKIQNFAKSALVKPVTINVGRAGAASLDVIQEVEYVKEEAKMVYLLECLQKT-PPPVLIFAEKKADVDAIHEYLLLKGVEAVAIHGGKDQEERTKAIEAFREGKKDVLVATDVASKGLDFPAIQHVINYDMPEEIENYVHRIGRTGRSGNTGIATTFINKACDESVLM 546

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. UniProt/SwissProt
Match: sp|Q9BUQ8|DDX23_HUMAN (Probable ATP-dependent RNA helicase DDX23 OS=Homo sapiens OX=9606 GN=DDX23 PE=1 SV=3)

HSP 1 Score: 746.888 bits (1927), Expect = 0.000e+0
Identity = 364/570 (63.86%), Postives = 461/570 (80.88%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            RYLG  +  +R +   N+RKFVF WD  EDTS DYNPLYK++H+ Q  GRG I GID+  QK+   +FY  +M  RR+  +K+QE  RL+ +  +EAK +WD+R W++K LDEMT+RDWRIFRED+SITTKGG IPN IR W+++ L   +L+VI +K  YKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL WI  LP++ ++E+++ GPYAIIL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G EIVIATPGRL+DVLENRY+VL++CTYV++DE DRMIDM FE DV+KIL ++P + +KPDTD+AED EK+L NF +   KYRQTVMFTATM P+VERLAR++LRRPA VYIGS GKP ER +Q +++++E++KR KL  I+ + F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI+DVS+V+NYDM K I++YIHR+GRTGRAGK G+AI+F+TK+DS +FY+LK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:  253 RYLGGIKKRRRTRHL-NDRKFVFEWDASEDTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEARLRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWKDSSLPPHILEVI-DKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEHMPVSNQKPDTDEAEDPEKMLANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSESEKRKKLLAILEQGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSAVFYELKQAILESPVSSCPPELANHPDAQHKPGTILTKKRR-EETIF 819

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. UniProt/SwissProt
Match: sp|Q5RC67|DDX23_PONAB (Probable ATP-dependent RNA helicase DDX23 OS=Pongo abelii OX=9601 GN=DDX23 PE=2 SV=1)

HSP 1 Score: 741.88 bits (1914), Expect = 0.000e+0
Identity = 362/570 (63.51%), Postives = 459/570 (80.53%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            RYLG  +  +R +   N+RKFVF WD  EDTS DYNPLYK++H+ Q  GRG I GID+  QK+   +FY  +M  RR+  +K+QE  R + +  +EAK +WD+R W++K LDEMT+RDWRIFRED+SITTKGG IPN IR W+++ L   +L+VI +K  YKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL WI  LP++ ++E+++ GPYAIIL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G EIVIATPGRL+DVLENRY+V ++CTYV++DE DRMIDM FE DV+KIL ++P + +KPDTD+AED EK+L NF +   KYRQTVMFTATM P+VERLAR++LRRPA VYIGS GKP ER +Q +++++E++KR KL  I+ + F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI+DVS+V+NYDM K I++YIHR+GRTGRAGK G+AI+F+TK+DS +FY+LK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:  253 RYLGGIKKRRRTRHL-NDRKFVFEWDASEDTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEARFRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWKDSSLPPHILEVI-DKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVPSRCTYVVLDEADRMIDMGFEPDVQKILEHMPVSNQKPDTDEAEDPEKMLANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSESEKRKKLLAILEQGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSAVFYELKQAILESPVSSCPPELANHPDAQHKPGTILTKKRR-EETIF 819

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. UniProt/SwissProt
Match: sp|Q5BCU6|PRP28_EMENI (Pre-mRNA-splicing ATP-dependent RNA helicase prp28 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=prp28 PE=3 SV=2)

HSP 1 Score: 540.421 bits (1391), Expect = 4.268e-173
Identity = 262/522 (50.19%), Postives = 370/522 (70.88%), Query Frame = 1

Query:  745 NERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWD--------ERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKME-QNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLT-ENQKRDKLREIMSR-NFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYD 2277
             +RKF F W+  EDTS DYNPLY+ +HEA FFGRG + G   +    ++ + Y K + DR      D+EA  ++     E + +          ++ W+EK L+ M  RDWRIF+EDF+I TKGG++PN +R W+E+GL  +LL+ ++++  YKEPTPIQR AIPI +Q+RD+IGVA TGSGKTAAFL+PLL +I +LPR+ + E +   GPYAI+L PTR+LA QI  E  KF +PLG   V+I+GG+S EEQ + LR GAEI+IATPGRLVD +E R +VL+QC YVIMDE DRMID+ FE  V KIL  LP   EKPDT++AED+  + ++  +  +YRQT+M+TATM  +VER+AR +LRRPA V IGS G+  +  +Q + M+  E++++ +L EI+S  +F PPII+FVN K+  D +A+ +++    +++ HG K QE RE A+A++++   D+LVAT+LAGRGID+ DVSLV+N++M  +I+ Y HR+GRTGRAGK G+AI+F+  +D+ + YD
Sbjct:  222 TDRKFNFEWNAEEDTSGDYNPLYQHRHEANFFGRGRLAGFGDDVAD-SVAKKYAKALEDR------DREAGSIRAREILEMERRRREESTRNQLDKHWSEKKLEHMRERDWRIFKEDFNIATKGGSVPNPMRSWDESGLPKRLLE-LVDRVGYKEPTPIQRAAIPIAMQSRDLIGVAVTGSGKTAAFLLPLLCYIAELPRIDEFEWRKNDGPYAIVLAPTRELAQQIEIEAKKFTEPLGFNVVSIVGGHSFEEQAYSLRNGAEIIIATPGRLVDCIERRMLVLSQCCYVIMDEADRMIDLGFEEPVNKILDALPVTNEKPDTEEAEDSSAMSRHLGSKDRYRQTMMYTATMPTAVERIARKYLRRPAIVTIGSAGEAVDTVEQRVEMIAGEDKRKKRLGEILSSGDFRPPIIVFVNIKRNCDAIAREIKQWGFSSVTLHGSKTQEQREAALASVRNGSTDVLVATDLAGRGIDVPDVSLVINFNMATSIESYTHRIGRTGRAGKSGVAITFLGNEDADVMYD 735

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. UniProt/SwissProt
Match: sp|Q2UH00|PRP28_ASPOR (Pre-mRNA-splicing ATP-dependent RNA helicase prp28 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=prp28 PE=3 SV=1)

HSP 1 Score: 539.265 bits (1388), Expect = 2.518e-172
Identity = 260/515 (50.49%), Postives = 371/515 (72.04%), Query Frame = 1

Query:  748 ERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDR--RSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKME-QNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQ-VIYMLTENQKRDKLREIMSRN-FVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYD 2277
            +RKF F W+  EDTS DYNPLY+ +HEA FFGRG + G   +     + + Y + + DR   + G + +E   ++     E+     ++ W+EK L+ M  RDWRIF+EDF+I+TKGG++PN +R W+E+GL  +L++ ++NK  YKEPTPIQR AIPI +Q+RD+IGVA TGSGKTA+FL+PLL +I +LPR+ + E +   GPYAI+L PTR+LA QI  E  KF +PLG   V+I+GG+S EEQ + LR GAEI+IATPGRLVD +E R +VL+QC YVIMDE DRMID+ FE  V KIL  LP + EKPD+++AE++  + Q+  T  +YRQT+M+TATM  +VER+AR +LRRPA V IGS G+  +  +Q V ++  E++++ +L +I+S   F PPII+FVN K+  D +A+ +++    +++ HG K QE RE A+A++++ + D+LVAT+LAGRGID+ DVSLV+N++M  TI+ Y HR+GRTGRAGK G+AI+F+  +D+ + YD
Sbjct:  244 DRKFNFEWNAEEDTSGDYNPLYQHRHEANFFGRGRLAGFGDDVADN-VAKKYARALEDRDHEAGGIRAREILEMERRRREESTRNQLDKHWSEKKLEHMRERDWRIFKEDFNISTKGGSVPNPMRSWDESGLPKRLME-LVNKVGYKEPTPIQRAAIPIAMQSRDLIGVAVTGSGKTASFLLPLLVYIAELPRIDEFEWRKNDGPYAIVLAPTRELAQQIEIEAKKFTEPLGFNVVSIVGGHSFEEQAYSLRNGAEIIIATPGRLVDCIERRMLVLSQCCYVIMDEADRMIDLGFEEPVNKILDALPVSNEKPDSEEAENSMAMSQHIGTKDRYRQTMMYTATMPTAVERIARKYLRRPAIVTIGSAGEAVDTVEQRVEFIAGEDKRKKRLGDILSSGEFRPPIIVFVNIKRNCDAIAREIKQWGFSSVTLHGSKTQEQREAALASVRNGQTDVLVATDLAGRGIDVPDVSLVINFNMATTIESYTHRIGRTGRAGKSGVAITFLGNEDTDVMYD 756

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. UniProt/SwissProt
Match: sp|A1CX72|PRP28_NEOFI (Pre-mRNA-splicing ATP-dependent RNA helicase prp28 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=prp28 PE=3 SV=1)

HSP 1 Score: 538.88 bits (1387), Expect = 2.789e-172
Identity = 263/521 (50.48%), Postives = 369/521 (70.83%), Query Frame = 1

Query:  748 ERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWD--------ERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKME-QNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLT-ENQKRDKLREIMSRN-FVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYD 2277
            +RKF F W+  EDTS DYNPLY+++HEA F+GRG + G   +     L Q Y + + DR      D+EA  ++     E + +          ++ W+EK L+ M  RDWRIF+EDF+I+TKGG++PN +R WEE+GL  +LL+ ++++  YKEPTPIQR AIPI LQ+RD+IGVA TGSGKTA+FL+PLL +I +LPR+ + E +   GPYAI+L PTR+LA QI  E  KF QPLG   V+I+GG+S EEQ + LR GAEI+IATPGRLVD +E R +VL+QC YVIMDE DRMID+ FE  V KIL  LP   EKPDT++AE++  +      + +YRQT+M+TATM  +VER+AR +LRRPA V IGS G+  +  +Q + M+  E++++ +L +I+S   F PPII+FVN K+  D +A+ +++M   +++ HG K QE RE A+A++++   D+LVAT+LAGRGID+ DVSLV+N++M  +I+ Y HR+GRTGRAGK G+AI+F+  +D+ + YD
Sbjct:  241 DRKFNFEWNAEEDTSGDYNPLYQQRHEANFYGRGRLAGFGDDVAD-TLAQKYARALEDR------DREAGSIRAREILEMERRRREESTRNQLDKHWSEKKLEHMRERDWRIFKEDFNISTKGGSVPNPMRSWEESGLPKRLLE-LVDQVGYKEPTPIQRAAIPIALQSRDLIGVAVTGSGKTASFLLPLLVYISELPRIDEFEWRKNDGPYAIVLAPTRELAQQIEIEARKFTQPLGFNVVSIVGGHSFEEQAYSLRNGAEIIIATPGRLVDCIERRLLVLSQCCYVIMDEADRMIDLGFEEPVNKILDALPVTNEKPDTEEAENSAAM-----RSHRYRQTMMYTATMPSAVERIARKYLRRPAIVTIGSAGEAVDTVEQRVEMIAGEDKRKKRLADILSSGEFRPPIIVFVNIKRNCDAIAREIKQMGFSSVTLHGSKTQEQREAALASVRNGSTDVLVATDLAGRGIDVPDVSLVVNFNMANSIESYTHRIGRTGRAGKSGVAITFLGNEDADVMYD 748

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. TrEMBL
Match: G4V9R0 (Putative dead box ATP-dependent RNA helicase OS=Schistosoma mansoni OX=6183 GN=Smp_093690.1 PE=3 SV=1)

HSP 1 Score: 816.994 bits (2109), Expect = 0.000e+0
Identity = 444/690 (64.35%), Postives = 544/690 (78.84%), Query Frame = 1

Query:  364 AVPKHAQIPLSVEEVLXXXXXXXXXXXXXXYFSKAEREALAIQRRXXXXXXXXXXXXXXXNLQTKYIEEG----RIKXXXXXXXXXXXXXXXXXXXKTAXXXXXXX-----XXXXXXXXXRYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLYI 2406
             VPK  ++P+S+EE++  K AEE    KPK+ SK ER   AIQRR+ +V+  ++K +     QT+Y+E      R +  +  +   R + + R+  K A Q E         +EE+AIKERYLGQKRLTKRR RRLNERKFVF WD  +DTS DYNPLYK+KH+ QFFGRGHIGGIDI  QKK +G+FY+K++  RRSD QK QE KRL  ++ REAK KWD+R WTEK LD+MT RDWRIFREDF+I+TKGGNIPN +R W E  ++D+L DVI  K  Y EPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL+WI++LP+L+++E  E GPYAII+ PTR+LA QI EET+KFG+PLGIK V++IGG SRE+Q  +LR+GAEIVI TPGRL DVLENRY+VLNQCTY+++DE D+MIDM FE +V  IL+YLP   EKPD +DAED  KLL NF T  KYRQTVMFTATM P+VERLAR++LRRPA VYIGS GKPTER +Q++YM++E +KR KL EI++    PP+IIFVNQKKGADVLAKGLEK+   A+  HGGKGQE REYA+A+LKS +K+ILVAT++AGRGIDIKDVS+V+NYDM KTIDEY+HR+GRTGRAGK G+AIS +TK+D+P+FYDLK+LL +SP+S  P EL +HPDAQ K G   +KKRR EET+YI
Sbjct:   43 CVPK--RVPISLEELILKKKAEEAELIKPKFISKEERIQQAIQRRQAEVQAQREKQMDAFKKQTEYLEGAKDLERKRRTEDFQERMRQNRNFRNTSKRAAQGEVPSDKINNSQEEQAIKERYLGQKRLTKRRPRRLNERKFVFDWDVADDTSQDYNPLYKEKHQIQFFGRGHIGGIDIKQQKKEIGRFYSKLLESRRSDTQKVQEKKRLSGVAKREAKQKWDDRHWTEKALDQMTERDWRIFREDFNISTKGGNIPNPLRSWAEMNVADELKDVI-KKVGYPEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLNWIQRLPKLERLEDTEQGPYAIIMAPTRELAQQIEEETVKFGRPLGIKTVSLIGGLSREDQALKLRMGAEIVIGTPGRLNDVLENRYMVLNQCTYIVLDEADKMIDMGFEPEVNNILTYLPVTNEKPDNEDAEDDSKLLSNFATKHKYRQTVMFTATMPPAVERLARSYLRRPAMVYIGSAGKPTERVEQIVYMVSEQEKRRKLLEILAAGLDPPVIIFVNQKKGADVLAKGLEKLGYSAVVLHGGKGQEQREYALASLKSGQKEILVATDVAGRGIDIKDVSMVINYDMSKTIDEYVHRIGRTGRAGKSGIAISLLTKEDAPVFYDLKQLLIQSPVSTCPHELANHPDAQTKPGILAAKKRRAEETVYI 729

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. TrEMBL
Match: G4V9R1 (Putative dead box ATP-dependent RNA helicase OS=Schistosoma mansoni OX=6183 GN=Smp_093690.2 PE=3 SV=1)

HSP 1 Score: 816.224 bits (2107), Expect = 0.000e+0
Identity = 442/690 (64.06%), Postives = 545/690 (78.99%), Query Frame = 1

Query:  364 AVPKHAQIPLSVEEVLXXXXXXXXXXXXXXYFSKAEREALAIQRRXXXXXXXXXXXXXXXNLQTKYIEEG----RIKXXXXXXXXXXXXXXXXXXXKTAX-----XXXXXXXXXXXXXXXRYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLYI 2406
             VPK  ++P+S+EE++  K AEE    KPK+ SK ER   AIQRR+ +V+  ++K +     QT+Y+E      R +  +  +   R + + R+  K A       ++ +  +EE+AIKERYLGQKRLTKRR RRLNERKFVF WD  +DTS DYNPLYK+KH+ QFFGRGHIGGIDI  QKK +G+FY+K++  RRSD QK QE KRL  ++ REAK KWD+R WTEK LD+MT RDWRIFREDF+I+TKGGNIPN +R W E  ++D+L DVI  K  Y EPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL+WI++LP+L+++E  E GPYAII+ PTR+LA QI EET+KFG+PLGIK V++IGG SRE+Q  +LR+GAEIVI TPGRL DVLENRY+VLNQCTY+++DE D+MIDM FE +V  IL+YLP   EKPD +DAED  KLL NF T  KYRQTVMFTATM P+VERLAR++LRRPA VYIGS GKPTER +Q++YM++E +KR KL EI++    PP+IIFVNQKKGADVLAKGLEK+   A+  HGGKGQE REYA+A+LKS +K+ILVAT++AGRGIDIKDVS+V+NYDM KTIDEY+HR+GRTGRAGK G+AIS +TK+D+P+FYDLK+LL +SP+S  P EL +HPDAQ K G   +KKRR EET+YI
Sbjct:  106 CVPK--RVPISLEELILKKKAEEAELIKPKFISKEERIQQAIQRRQAEVQAQREKQMDAFKKQTEYLEGAKDLERKRRTEDFQERMRQNRNFRNTSKRAAQGEVPSDKINNSQEEQAIKERYLGQKRLTKRRPRRLNERKFVFDWDVADDTSQDYNPLYKEKHQIQFFGRGHIGGIDIKQQKKEIGRFYSKLLESRRSDTQKVQEKKRLSGVAKREAKQKWDDRHWTEKALDQMTERDWRIFREDFNISTKGGNIPNPLRSWAEMNVADELKDVI-KKVGYPEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLNWIQRLPKLERLEDTEQGPYAIIMAPTRELAQQIEEETVKFGRPLGIKTVSLIGGLSREDQALKLRMGAEIVIGTPGRLNDVLENRYMVLNQCTYIVLDEADKMIDMGFEPEVNNILTYLPVTNEKPDNEDAEDDSKLLSNFATKHKYRQTVMFTATMPPAVERLARSYLRRPAMVYIGSAGKPTERVEQIVYMVSEQEKRRKLLEILAAGLDPPVIIFVNQKKGADVLAKGLEKLGYSAVVLHGGKGQEQREYALASLKSGQKEILVATDVAGRGIDIKDVSMVINYDMSKTIDEYVHRIGRTGRAGKSGIAISLLTKEDAPVFYDLKQLLIQSPVSTCPHELANHPDAQTKPGILAAKKRRAEETVYI 792

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. TrEMBL
Match: A0A0R3WBU9 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS8136 PE=4 SV=1)

HSP 1 Score: 812.372 bits (2097), Expect = 0.000e+0
Identity = 439/699 (62.80%), Postives = 548/699 (78.40%), Query Frame = 1

Query:  376 HAQIPLSVEEVLXXXXXXXXXXXXXXYFSKAEREALAIQRRXXXXXXXXXXXXXXXNLQTKYIEEGRIKXXXXXXXXXXXXXXXXXXXK---------------------TAXXXXXXXXXXXXXXXXRYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLYIA 2409
             A++P+S+EE++  K A +    KPK+ S+ ERE +A+QRREE ++E +K+A  E + Q +Y+ + RI S + D  SR      R   +                      ++ +  DK REEEAIKERYLGQKRLTKRRQRRLNERKFVF WD  EDTS DYNPLYK+KH+ QFFGRGHIGGIDI  QK+ +G+FY+KM+  RRSD QK+QE KRL  ++ REAK KWD+R W+EK L EMT RDWRIFRED++I+TKGGN+PN IR W+E+ +   LL+ II+K  Y EPT IQRQAIPIGLQNRDIIG+AETGSGKT AFL+PLL+WI+ LP+L +ME  E GPYA+I+ P+R+LA QI EET+KFG PLGIK V +IGG S+E+Q  +LR+GAEIVI TPGRLVDVLE+RYIVLNQCTYV++DE D+MIDM FE  V  ILSYLP   +KPDT++AE  EKLL NF T QKYRQTVMFTATM P+VERLAR++LRRPATVY+G+ GKPT+R +Q++Y++ EN KR KL EI++    PP+IIFVNQKKGAD+LAKGLEK+   A+  HGGKGQEHREYA+A+LKS +K+ILVAT++AGRGIDIKDVS+V+NYDM KTID+Y+HR+GRTGRAGK G+A++F+TKDD+ ++YDLK+LL  SP+S  P EL +HPDAQ K GTF +KKRRN+ET+YIA
Sbjct:  134 QAKVPVSLEELMQRKEAADAELSKPKFISREEREKMALQRREEAIREQRKRAADELSKQIEYLSKTRIDSSNLDSLSRYDRDSYRLRRRVNEWDRRYPDKGRDRRAEDGSASKVSAADKVREEEAIKERYLGQKRLTKRRQRRLNERKFVFDWDANEDTSQDYNPLYKEKHQIQFFGRGHIGGIDIKQQKREIGKFYSKMLESRRSDTQKEQENKRLSGLAKREAKEKWDDRHWSEKSLSEMTYRDWRIFREDYNISTKGGNLPNPIRSWKESAIHKDLLE-IIHKVGYSEPTAIQRQAIPIGLQNRDIIGIAETGSGKTLAFLVPLLTWIQTLPKLVRMEDTEQGPYALIMAPSRELAQQIEEETVKFGAPLGIKTVAVIGGISKEDQALKLRMGAEIVIGTPGRLVDVLESRYIVLNQCTYVVLDEADKMIDMGFEPHVNNILSYLPVTNQKPDTEEAEQDEKLLANFSTKQKYRQTVMFTATMPPAVERLARSYLRRPATVYVGTAGKPTDRVEQIVYIVQENDKRKKLLEILNEGIEPPVIIFVNQKKGADMLAKGLEKLGHSAVVLHGGKGQEHREYALASLKSGQKEILVATDVAGRGIDIKDVSMVINYDMAKTIDDYVHRIGRTGRAGKSGIAVTFLTKDDTAVYYDLKQLLLTSPVSTCPPELANHPDAQTKPGTFAAKKRRNDETVYIA 831

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. TrEMBL
Match: A0A4E0RA85 (DEAD box ATP dependent RNA helicase OS=Fasciola hepatica OX=6192 GN=D915_004945 PE=4 SV=1)

HSP 1 Score: 811.216 bits (2094), Expect = 0.000e+0
Identity = 446/726 (61.43%), Postives = 559/726 (77.00%), Query Frame = 1

Query:  259 KESFPDIEDFEVEKKIPFNDQSKQNNENAVFKTPNAVPKHAQIPLSVEEVLXXXXXXXXXXXXXXYFSKAEREALAIQRRXXXXXXXXXXXXXXXNLQTKYIEEGR----------IKXXXXXXXXXXXXXXXXXXXKTAXXXXXXXXXXXXXXXXRYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLYI 2406
            ++ FP  +  + E K+   + S   ++N V K+    PK  ++P+S+E+++A K AEE    KPK+ SK ER+A A++RR+ +V+  +++ L +   QT+Y+E+ R          I+      R R       +  +     E+D+ +E EAIKERYLGQKRLTKRRQRRLNERKFVF WD  EDTS DYNPLYK+KH+ QFFGRGHIGGIDI  QKK +G+FY +++ DRRSD QK QE +RL  ++ REAK KWD+R W+EK L +MT RDWRIFRED+ I TKGGNIP  IR W+E G S +LL  II K  Y EPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL+WI++LP+L++ME  E GPYAII+ PTR+LA QI EET+KFG+PLGIK V++IGG SRE+Q  +LR+GAEIVIATPGRL DVLENRY+VLNQCTYV++DE D+MIDM FE +V  ILSYLP   EKPDT+DAED  KLL NF T  KYRQTVMFTATM PSVERLAR++LRRPA VYIGS GKPTER +Q++YM++E +KR KL +I++    PP+IIFVNQKKGAD+LAKGL+K+   A+  HGGK QE REYA+A+LKS +K+ILVAT++AGRGIDIKDVSLV+NYDM KTID+Y+HR+GRTGRAGK G AI+ +TK+D+P++YDLK+LL +SP+S  P +L +HPDAQ K G   +KKRR +ET+YI
Sbjct:  156 RKPFPPHQAAKSEPKL---ETSTTGDKNTVTKS-TLPPK--KVPISLEDLIAKKKAEEAELVKPKFISKEERQAEALRRRQIEVQAQRERQLADLKKQTEYLEQAREAERSRRSEYIERDYRRARGRGIQNKPSNDSQAHRNTEKDETKEMEAIKERYLGQKRLTKRRQRRLNERKFVFDWDAAEDTSQDYNPLYKEKHQIQFFGRGHIGGIDIKQQKKEIGRFYARLLGDRRSDTQKVQEERRLVGVARREAKQKWDDRHWSEKALSQMTERDWRIFREDYGIATKGGNIPCPIRSWDEMGGSPELLQ-IIRKVGYTEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLNWIQRLPKLERMEDTEQGPYAIIMAPTRELAQQIEEETVKFGRPLGIKTVSLIGGLSREDQALKLRMGAEIVIATPGRLNDVLENRYLVLNQCTYVVLDEADKMIDMGFEPEVNNILSYLPVTNEKPDTEDAEDDSKLLSNFATKHKYRQTVMFTATMPPSVERLARSYLRRPAVVYIGSAGKPTERVEQIVYMVSEQEKRKKLLQILNGGIDPPVIIFVNQKKGADMLAKGLDKLGHSAVVLHGGKNQEQREYALASLKSGQKEILVATDVAGRGIDIKDVSLVINYDMSKTIDDYVHRIGRTGRAGKSGTAITLLTKEDTPVYYDLKQLLLQSPVSTCPHDLANHPDAQTKPGIAAAKKRRADETVYI 874

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. TrEMBL
Match: A0A2H1CBR0 (DEAD/DEAH box helicase OS=Fasciola hepatica OX=6192 GN=D915_07936 PE=4 SV=1)

HSP 1 Score: 810.061 bits (2091), Expect = 0.000e+0
Identity = 446/726 (61.43%), Postives = 559/726 (77.00%), Query Frame = 1

Query:  259 KESFPDIEDFEVEKKIPFNDQSKQNNENAVFKTPNAVPKHAQIPLSVEEVLXXXXXXXXXXXXXXYFSKAEREALAIQRRXXXXXXXXXXXXXXXNLQTKYIEEGR----------IKXXXXXXXXXXXXXXXXXXXKTAXXXXXXXXXXXXXXXXRYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLYI 2406
            ++ FP  +  + E K+   + S   ++N V K+    PK  ++P+S+E+++A K AEE    KPK+ SK ER+A A++RR+ +V+  +++ L +   QT+Y+E+ R          I+      R R       +  +     E+D+ +E EAIKERYLGQKRLTKRRQRRLNERKFVF WD  EDTS DYNPLYK+KH+ QFFGRGHIGGIDI  QKK +G+FY +++ DRRSD QK QE +RL  ++ REAK KWD+R W+EK L +MT RDWRIFRED+ I TKGGNIP  IR W+E G S +LL  II K  Y EPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL+WI++LP+L++ME  E GPYAII+ PTR+LA QI EET+KFG+PLGIK V++IGG SRE+Q  +LR+GAEIVIATPGRL DVLENRY+VLNQCTYV++DE D+MIDM FE +V  ILSYLP   EKPDT+DAED  KLL NF T  KYRQTVMFTATM PSVERLAR++LRRPA VYIGS GKPTER +Q++YM++E +KR KL +I++    PP+IIFVNQKKGAD+LAKGL+K+   A+  HGGK QE REYA+A+LKS +K+ILVAT++AGRGIDIKDVSLV+NYDM KTID+Y+HR+GRTGRAGK G AI+ +TK+D+P++YDLK+LL +SP+S  P +L +HPDAQ K G   +KKRR +ET+YI
Sbjct:  112 RKPFPPHQAAKSEPKL---ETSTTGDKNTVTKS-TLPPK--KVPISLEDLIAKKKAEEAELVKPKFISKEERQAEALRRRQIEVQAQRERQLADLKKQTEYLEQAREAERSRRSEYIERDYRRARGRGIQNKPSNDSQAHRNTEKDETKEMEAIKERYLGQKRLTKRRQRRLNERKFVFDWDAAEDTSQDYNPLYKEKHQIQFFGRGHIGGIDIKQQKKEIGRFYARLLGDRRSDTQKVQEERRLVGVARREAKQKWDDRHWSEKALSQMTERDWRIFREDYGIATKGGNIPCPIRSWDEMGGSPELLQ-IIRKVGYTEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLNWIQRLPKLERMEDTEQGPYAIIMAPTRELAQQIEEETVKFGRPLGIKTVSLIGGLSREDQALKLRMGAEIVIATPGRLNDVLENRYLVLNQCTYVVLDEADKMIDMGFEPEVNNILSYLPVTNEKPDTEDAEDDSKLLSNFATKHKYRQTVMFTATMPPSVERLARSYLRRPAVVYIGSAGKPTERVEQIVYMVSEQEKRKKLLQILNGGIDPPVIIFVNQKKGADMLAKGLDKLGHSAVVLHGGKNQEQREYALASLKSGQKEILVATDVAGRGIDIKDVSLVINYDMSKTIDDYVHRIGRTGRAGKSGTAITLLTKEDTPVYYDLKQLLLQSPVSTCPHDLANHPDAQTKPGIAAAKKRRADETVYI 830

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Cavefish
Match: ddx23 (DEAD-box helicase 23 [Source:NCBI gene;Acc:103037499])

HSP 1 Score: 746.888 bits (1927), Expect = 0.000e+0
Identity = 369/570 (64.74%), Postives = 455/570 (79.82%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            RYLG  +  +R +   N+RKFVF WD  EDTS DYNPLYK+KH+ Q +GRG I GID+  QK+    FY  +M  RR+  +K+QE  RLK +  +EAK +WD+R W++K LDEMT+RDWRIFRED+SITTKGG IPN IR W+E  L   +L+VI +K  YKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL WI  LP++ ++E ++ GPYA+IL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G EIVIATPGRL+DVLENRY+VL +CTYV++DE DRMIDM FE DV+KIL Y+P   +KPDTD+AED EK+ QNF +   KYRQTVMFTATM P+VERLAR++LRR A VYIGS GKP ER +Q + +++E +KR KL E++SR F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI DVS+VLNYDM K I++YIHR+GRTGRAGK G+A++F+TK+DS +FYDLK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:  246 RYLGGMKKRRRTRHL-NDRKFVFEWDASEDTSIDYNPLYKEKHQVQLYGRGFIAGIDLKQQKRDQSHFYGDLMEKRRTMEEKEQEELRLKKMRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRNWKEFDLPPHILEVI-DKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEDSDQGPYAVILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLGRCTYVVLDEADRMIDMGFEPDVQKILEYIPVTNQKPDTDEAEDPEKMTQNFESGKHKYRQTVMFTATMPPAVERLARSYLRRAAVVYIGSAGKPHERVEQKVILMSEGEKRKKLLEVLSRGFEPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIHDVSMVLNYDMAKNIEDYIHRIGRTGRAGKSGVAMTFLTKEDSSVFYDLKQAILESPVSTCPPELTNHPDAQHKPGTILTKKRR-EETIF 812

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Cavefish
Match: ddx46 (DEAD-box helicase 46 [Source:NCBI gene;Acc:103041720])

HSP 1 Score: 288.5 bits (737), Expect = 3.312e-80
Identity = 163/419 (38.90%), Postives = 246/419 (58.71%), Query Frame = 1

Query: 1024 HLDEMTNRDWRIFREDF-SITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWI-EKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVL---ENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSR-NFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDS 2262
             L  MT  +   +R +   IT KG   P  I+ W + G S K+L+ +  K  Y++PTPIQ QAIP  +  RD+IG+A+TGSGKT AFL+P+   I ++ P    +E+ E GP A+I+ PTR+LA+QI +E  KF +PLG++ V + GG    EQ+ +L+ GAEI++ TPGR++D+L     R   L + TYV++DE DRM DM FE  V +I+  +     +PD                    RQTVMF+AT   ++E LAR  L +P  V +G         +Q + ++ E++K  KL EI+        +IIFV++++ AD L K L K +   +S HGG  Q  R+  +   K+    ++VAT++A RG+D+K + LV+NY+ P   ++Y+HR GRTGRAG +G A +F+T + +
Sbjct:  343 ELARMTPEEVNEYRLELEGITVKGKGCPKPIKTWVQCGTSMKVLNAL-KKHNYEKPTPIQSQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHILDQRP----LEEGE-GPIAVIMTPTRELALQITKECKKFSKPLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLGANSGRVTNLRRVTYVVVDEADRMFDMGFEPQVMRIMDNV-----RPD--------------------RQTVMFSATFPRAMEALARRILNKPVEVQVGGRSVVCSDVEQHVIVIEEDKKFLKLLEILGHYQEKGSVIIFVDKQEHADGLLKDLMKASYPCMSLHGGIDQYDRDSIINDFKNGACRLMVATSVAARGLDVKQLILVVNYNCPNHYEDYVHRAGRTGRAGNKGFAYTFITDEQA 730

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Cavefish
Match: DDX5 (probable ATP-dependent RNA helicase DDX5 [Source:NCBI gene;Acc:103046784])

HSP 1 Score: 263.848 bits (673), Expect = 3.594e-75
Identity = 159/460 (34.57%), Postives = 246/460 (53.48%), Query Frame = 1

Query:  922 RRSDGQKDQEAKRLKNISNREAKVKW--DERPWTEKHL----DEMTNRDWR---IFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIG-KPTERTKQVIYMLTENQKRDK----LREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDD 2259
            R   G+      +  N   R  K  W  DE P  EK+      ++T R  +    +R    +T KG + P  +  + EA   + +++VI NK  + EPTPIQ Q  P+ L  +D++G+A+TGSGKT A+L+P +  I   P L+  +    GP  ++L PTR+LA Q+ +   ++G+   +K+  I GG  +  Q+  L  G EI IATPGRL+D LE     L +CTY+++DE DRM+DM FE  +RKI+  +     +PD                    RQT+M++AT    V +LA  FL+    + IG++         Q++ +  + +K DK    L EIMS       IIFV  K+  D L + + +    A+  HG K Q+ R++ +   K  +  IL+AT++A RG+D++DV  V+NYD P + ++YIHR+GRT R+ K G A +F T ++
Sbjct:   21 RFGSGRSGPPTSKFGNPGERLRKRHWNLDELPKFEKNFYKEHPDITRRSMQEVEQYRRAKEVTVKGRDCPKPVMKFNEATFPNYVMEVI-NKQAWSEPTPIQAQGWPLALSGKDMVGIAQTGSGKTLAYLLPAIVHINHQPFLEHGD----GPICLVLAPTRELAQQVQQVAAEYGRASRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLEAGKTNLRRCTYLVLDEADRMLDMGFEPQIRKIVDQI-----RPD--------------------RQTLMWSATWPKEVRQLAEDFLKEYVQINIGALQLSANHNILQIVDVCNDGEKDDKLIRLLEEIMSEK-ENKTIIFVETKRRCDELTRRMRRDGWPAMGIHGDKSQQERDWVLNEFKYGKACILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSQKTGTAYTFFTPNN 449

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Cavefish
Match: ddx3xb (DEAD-box helicase 3 X-linked b [Source:ZFIN;Acc:ZDB-GENE-980526-150])

HSP 1 Score: 259.61 bits (662), Expect = 1.028e-73
Identity = 145/412 (35.19%), Postives = 224/412 (54.37%), Query Frame = 1

Query: 1066 EDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLS--WIEKLPRLQKMEQNELG-----PYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPI-IIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYD 2277
            +D  +   G N P  I  + +  + + ++  I   ++Y  PTP+Q+ AIPI    RD++  A+TGSGKTAAFL+P+LS  + +      + E    G     P +++L PTR+LA+QI +E  KF     ++   + GG    +Q+  L  G  +++ATPGRLVD++E   I L+ C Y+++DE DRM+DM FE  +R+I+                      Q+    +  RQT+MF+AT    ++ LAR FL     + +G +G  +E   Q +  + E+ KR  L ++++      + ++FV  KKGAD L   L +      S HG + Q  RE A+   +S    ILVAT +A RG+DI +V  V+N+D+P  I+EY+HR+GRTGR G  GLA SF    +S +  D
Sbjct:  122 DDIPVEATGHNSPPHIDSFHDVEMGEIIMGNIA-LSRYTRPTPVQKHAIPIIKTKRDLMACAQTGSGKTAAFLLPVLSQIYTDGPGEALQAENGRYGRRKQYPLSLVLAPTRELALQIYDEARKFAYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDYCNYLVLDEADRMLDMGFEPQIRRIVE---------------------QDTMPPKGLRQTMMFSATFPKEIQILARDFLEDYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLLNATGKDSLTLVFVETKKGADALEDFLYREGYACTSIHGDRSQRDREEALNQFRSGRCPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNDKNSNITKD 511

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Cavefish
Match: DDX41 (DEAD-box helicase 41 [Source:NCBI gene;Acc:103035623])

HSP 1 Score: 259.225 bits (661), Expect = 1.306e-73
Identity = 146/404 (36.14%), Postives = 229/404 (56.68%), Query Frame = 1

Query: 1063 REDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSW-IEKLPRLQKMEQNELGPYAIILVPTRDLAIQ---ILEETMKFGQPLG---IKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTK 2253
            R+ + I  +G  IP  I+ + E      +L  +  K     PTPIQ Q IP  L  RD+IG+A TGSGKT  F +P++ + +E+  RL   ++   GPY +I+ P+R+LA Q   I+E   K  +  G   +++   IGG S +EQ+  ++ G  +++ATPGRL+D+L  + + L+ C Y+ +DE DRMIDM FE D+R I SY                          +  RQT++F+ATM   ++  A++ L +P T+ +G  G  +    Q +  + E  K   L E + +   PP++IF  +K   D + + L    ++A++ HGGK QE R  A+ A K  +KD+LVAT++A +G+D   +  V+NYDMP+ I+ Y+HR+GRTGR+GK G+A +F+ K
Sbjct:  156 RKKYHILVEGDGIPAPIKSFREMKFPQAILKGL-KKKGIVHPTPIQIQGIPTILSGRDMIGIAFTGSGKTLVFTLPIIMFCLEQEKRLPFCKRE--GPYGLIICPSRELARQTHSIIEYYCKLLEDEGAPQMRSALCIGGMSVKEQMEVVKHGVHMMVATPGRLMDLLNKKMVSLDICRYLALDEADRMIDMGFEEDIRTIFSYF-------------------------KGQRQTLLFSATMPKKIQNFAKSALVKPITINVGRAGAASLDVIQEVEYVKEEAKMVYLLECLQKT-PPPVLIFAEKKADVDAIHEYLLLKGVEAVAIHGGKDQEERTKAIEAFKEGKKDVLVATDVASKGLDFPAIQHVINYDMPEEIENYVHRIGRTGRSGKTGVATTFINK 530

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Sea Lamprey
Match: ddx23 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 [Source:ZFIN;Acc:ZDB-GENE-030131-6215])

HSP 1 Score: 730.709 bits (1885), Expect = 0.000e+0
Identity = 394/682 (57.77%), Postives = 524/682 (76.83%), Query Frame = 1

Query:  388 PLSVEEVLXXXXXXXXXXXXXXYFSKAEREALAIQRRXXXXXXXXXXXXXXXNLQTKYIEEGR---------IKXXXXXXXXXXXXXXXXXXXKTAXXXXXXXXXXXXXXXXRYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            PLS+EE++A K AE+EAE+KPK+ +K EREA A++RR ++V+E ++    E+  +  + EEG+          +    +RR R +       D+   + E+D+++E +AIK+RYLGQ +  +R +R  N+RKFVF WD GEDTS D+NP+YK++H  Q +GRG++ GID+  QKK   +FY  ++  RR+  +KDQE  RLK    +EAK +WD+R W++K L+EM  RDWRIFRED++ITTKGG IPN IR W+E+ L   + +VI  K  YK+PTPIQRQAIPIGLQNRDIIG+AETGSGKTAAFLIPLL WI  LP++ ++E  + GPYA+IL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G +IVIATPGRL+DVLENRY+VL++CTYV++DE DRMIDM FE DV++IL ++P + +KPDTDDAED +K+  NF +   KYRQTVMFTATM  +VERLAR++LRRPA VYIGS+GKP ER +Q ++++ E++KR KL E++SR F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI DVS+VLNYDM K I++Y HR+GRTGRAGK G++++F+TK+D+ +FYDLK+ +  SP+S+ P EL +HPDAQ K GT  +KKRR EET++
Sbjct:   70 PLSLEEMIARKKAEQEAEQKPKFLTKVEREAEALRRRSQQVEEQRRAMEEERKKRMVFQEEGKKMMEDPYERDRRERRERRERETETSLNEDDRNKIREEKDREKEMQAIKDRYLGQPKKRRRVRRL-NDRKFVFDWDAGEDTSVDHNPIYKERHSVQLYGRGYVAGIDLKQQKKEQSKFYGDLLERRRTQEEKDQEEVRLKKERKKEAKQRWDDRHWSQKKLEEMCERDWRIFREDYNITTKGGKIPNPIRLWKESILPSHIREVI-EKCGYKDPTPIQRQAIPIGLQNRDIIGIAETGSGKTAAFLIPLLVWITTLPKIDRIEDTDQGPYAVILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCKIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQRILEHMPVSNQKPDTDDAEDPDKMTANFESGRHKYRQTVMFTATMPAAVERLARSYLRRPAVVYIGSVGKPIERVEQRVFIMGESEKRKKLLELLSRGFEPPIIIFVNQKKGCDVLAKSLEKMGFNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIHDVSMVLNYDMAKNIEDYTHRIGRTGRAGKSGVSVTFLTKEDAAVFYDLKQAMLGSPVSQCPPELANHPDAQHKPGTILTKKRR-EETIF 748

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Sea Lamprey
Match: ddx46 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 [Source:NCBI gene;Acc:321948])

HSP 1 Score: 278.485 bits (711), Expect = 1.178e-77
Identity = 153/415 (36.87%), Postives = 238/415 (57.35%), Query Frame = 1

Query: 1027 LDEMTNRDWRIFREDF-SITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVL---ENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMS-RNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKD 2256
            L +MT  +   +R +   +  KG   P  I+ W + G+S K+L+ +  K  Y++PTPIQ QAIP  +  RD+IG+A+TGSGKT  FL+P+   +     L++ E    GP  +I+ PTR+LA+QI +E  KF + L ++ V + GG    EQ+ +L+ GAEI++ TPGR++D+L     R     + TY+++DE DRM DM FE  V +I+      T +PD                    RQTVMF+AT   ++E LAR  L +P  V +G         +Q + ++ E+ K  KL E++        +I+FV++++ AD L K L K +   +S HGG  Q  R+  +   KS    ++VAT++A RG+D+K + +V+NYD P   ++Y+HRVGRTGRAG +G + +F+T +
Sbjct:  339 LAKMTAEEVSAYRLEMEGVMVKGKGCPKPIKTWVQCGISMKILNTL-KKQNYEKPTPIQAQAIPAIMSGRDLIGIAKTGSGKTIGFLLPMFRHVMDQRPLEEGE----GPIGVIMTPTRELALQIAKECRKFTKQLALRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNFRRATYMVLDEADRMFDMGFEPQVMRIIE-----TSRPD--------------------RQTVMFSATFPRAMEALARRILNKPVEVQVGGRSVVCSDVEQHVIVIEEDTKFYKLLELLGLYQEKGAVIVFVDKQEHADTLLKDLMKASYPCLSLHGGIDQYDRDSIINDFKSGVCRLMVATSVAARGLDVKHLIMVVNYDCPNHYEDYVHRVGRTGRAGNKGYSYTFITSE 723

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003898.1 (pep scaffold:Pmarinus_7.0:GL481305:13824:21767:-1 gene:ENSPMAG00000003524.1 transcript:ENSPMAT00000003898.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 257.684 bits (657), Expect = 1.448e-74
Identity = 145/406 (35.71%), Postives = 222/406 (54.68%), Query Frame = 1

Query: 1066 EDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWI---------EKLPRLQ---KMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPI-IIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISF 2244
            +D  +   G + P  I  + E  L + ++  +    +Y+ PTP+Q+ AIPI    RD++  A+TGSGKTAAFL+P+LS I         E     Q   K  Q +  P +++L PTR+LA+QI +E  KF     ++   + GG     QV +L+ G  +++ATPGRLVD++E   + L+ C Y+++DE DRM+DM FE  +R+I+                      Q+   T+  RQT+MF+AT    ++ LAR FL     + +G +G  +E   Q +  + E  KR  L ++++      + ++FV  KKGAD L   L        S HG + Q  RE A++  ++  + ILVAT +A RG+DI +V  V+N+D+P  I+EY+HR+GRTGR G  G A SF
Sbjct:   43 DDIPVEATGNDCPPHIESFHELELGEIIMSSV-TLCRYERPTPVQKYAIPIIKAKRDLMACAQTGSGKTAAFLLPILSQIYSGGPGKVVEDTKNQQETVKYGQRKQYPMSLVLAPTRELALQIYDEARKFAYRSCVRPCVVYGGADIGNQVRELQKGCHLLVATPGRLVDMMERGKVGLDHCRYLVLDEADRMLDMGFEPQIRRIVE---------------------QDVMPTKGDRQTLMFSATFPKEIQILARDFLEDYIFLAVGRVGSASENITQKVVWVEEMDKRSFLLDLLNATGRDSLTLVFVETKKGADALQGFLCSEGYSCTSIHGDRSQRDREEALSLFRTGRRPILVATAVAARGLDIPNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGQATSF 426

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Sea Lamprey
Match: ddx42 (DEAD (Asp-Glu-Ala-Asp) box helicase 42 [Source:ZFIN;Acc:ZDB-GENE-050706-53])

HSP 1 Score: 252.292 bits (643), Expect = 8.798e-71
Identity = 150/399 (37.59%), Postives = 217/399 (54.39%), Query Frame = 1

Query: 1090 GGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNEL----GPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKR-----DKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDD 2259
            G   P     +   G  ++LL+ +I K+++ +PTPIQ Q IPI L  RDIIG+A+TGSGKTA+F+ P+L  I        M Q EL    GP  +I VPTR+L  QI  E  +FG+   + +V + GG S  EQ   L+ GAEIV+ TPGRL+D ++ +   L + TY++ DE DRM DM FE  VR I +++     +PD                    RQT++++AT +  VE+LAR  L  P  V  G IG+  E   Q + + +    +       L E  S   V   +IFV +K  ++ L+  LE+   +    HG   Q  R   ++  K K   I+VAT++A RG+DI  +  V+NYD+ + ID + HRVGRTGRAG++G A + +T  D
Sbjct:  241 GAAPPKPCSSFGHFGFDEQLLN-LIRKSEFTQPTPIQCQGIPIALSGRDIIGIAKTGSGKTASFIWPMLVHI--------MAQKELKPGDGPIGLICVPTRELCQQINVECKRFGKAYNLHSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVTYLVFDEADRMFDMGFEYQVRSIANHV-----RPD--------------------RQTMLYSATFRMRVEKLARDILIDPIRVVQGDIGEANEDVTQRVEIFSNGAAKWNWLCSHLVEFTSSGSV---LIFVTRKDNSEELSNDLEQEGFQVGLLHGDMDQSERNKVISDFKKKVIPIMVATDVAARGLDIPSIKTVVNYDVARDIDTHTHRVGRTGRAGEKGTAFTLMTHKD 602

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000216.1 (pep scaffold:Pmarinus_7.0:GL476721:117971:126978:-1 gene:ENSPMAG00000000186.1 transcript:ENSPMAT00000000216.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 233.417 bits (594), Expect = 1.332e-67
Identity = 134/366 (36.61%), Postives = 200/366 (54.64%), Query Frame = 1

Query: 1177 YKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLEN-RYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLF 2271
            +K+PT IQ +AIP+ LQ +DIIG+AETGSGKT AF +P+L  +   P  Q++       +A++L PTR+LA QI E+    G  +GIK+  ++GG     Q   L     ++IATPGRLVD LEN +   +    Y++MDE DR+++M+FE +V KIL  +P                         K R+T +F+ATM   V++L R  L+ P    + +  +  E+ +Q    +    K   L  I++       IIF +       +A  L  +   AI  HG   Q  R  ++   K K + IL+AT++A RG+DI  V +V+N+D+P    +YIHRVGRT RAG+ G +I+FV++ D  LF
Sbjct:   15 WKQPTKIQVEAIPVALQGKDIIGLAETGSGKTGAFALPILQALLDAP--QRL-------FALVLTPTRELAFQISEQFEALGSSIGIKSAVVVGGIDMMSQSLALAKKPHVIIATPGRLVDHLENTKGFNIRALKYLVMDEADRILNMDFETEVDKILKVIP-------------------------KERKTYLFSATMTKKVQKLQRAALKEPVKCSVSTKYQTVEKLQQYYVFIPAKFKDSYLVYILNELAGNSFIIFCSTCNNTQRIALLLRNLGFTAIPLHGQMSQNKRLGSLNKFKGKARSILLATDVASRGLDIPHVDVVINFDIPTHSKDYIHRVGRTARAGRSGKSITFVSQYDVELF 346

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Yeast
Match: PRP28 (RNA binding protein; involved in RNA isomerization at the 5' splice site and for exchange of U6 for U1 snRNA at the 5' splice site; similar to the RNA helicases of the DEAD-box family [Source:SGD;Acc:S000002651])

HSP 1 Score: 332.028 bits (850), Expect = 2.823e-100
Identity = 197/514 (38.33%), Postives = 288/514 (56.03%), Query Frame = 1

Query:  745 NERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDK-LLDVIINKAKYKEPTPIQRQAIPIGL---QNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLG------IKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVI-YMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEK-MNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVT 2250
            N  KF F+W+E EDT + Y+P+                        +A+   +               + K  KN     A+  +  + WTEK L EM  RDWRI +ED++I TKGG + N +R WEE  +  + LL VII + ++  PTPIQR  IP      Q RD +GVA TGSGKT AF+IP+L  + + P      +   GP A+IL PTR+L  QI +ET K  +          K ++I+GG+S EE  F L  G +I++ATPGRL+D LEN  +V+ Q   +++DE D+MID+ FE  V  IL+ +       + D A +              RQT+MFTATM P +E++A  ++++P    IG         +QV+ Y   +  K  KL+ I+++ + PPIIIF+N K+ AD LA+  +K  N+K    HG K QE RE+++   ++ +  I++ATN+A RG+DI +VSLV+N+ + K +D+YIHR+GRTGRA  EG A+SFV+
Sbjct:   89 NGSKFHFSWNESEDTLSGYDPI---------------------VSTRAIDLLW---------------KGKTPKN----AAESSYMGKHWTEKSLHEMNERDWRILKEDYAIVTKGGTVENPLRNWEELNIIPRDLLRVIIQELRFPSPTPIQRITIPNVCNMKQYRDFLGVASTGSGKTLAFVIPILIKMSRSPPRPPSLKIIDGPKALILAPTRELVQQIQKETQKVTKIWSKESNYDCKVISIVGGHSLEEISFSLSEGCDILVATPGRLIDSLENHLLVMKQVETLVLDEADKMIDLGFEDQVTNILTKV-----DINADSAVN--------------RQTLMFTATMTPVIEKIAAGYMQKPVYATIGVETGSEPLIQQVVEYADNDEDKFKKLKPIVAK-YDPPIIIFINYKQTADWLAEKFQKETNMKVTILHGSKSQEQREHSLQLFRTNKVQIMIATNVAARGLDIPNVSLVVNFQISKKMDDYIHRIGRTGRAANEGTAVSFVS 542

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Yeast
Match: DBP2 (ATP-dependent RNA helicase of the DEAD-box protein family; has strong preference for dsRNA; interacts with YRA1; required for assembly of Yra1p, Nab2p and Mex67p onto mRNA and formation of nuclear mRNP; involved in mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites [Source:SGD;Acc:S000005056])

HSP 1 Score: 271.552 bits (693), Expect = 1.699e-79
Identity = 150/407 (36.86%), Postives = 236/407 (57.99%), Query Frame = 1

Query: 1060 FREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIG-KPTERTKQVIYMLTENQKRDKLR---EIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPL 2268
            FR++  +T  G +IP  I  ++EAG  D +L+ +  +  + +PT IQ Q  P+ L  RD++G+A TGSGKT ++ +P +  I   P L   +    GP  ++L PTR+LA+QI  E  KFG    I+   + GG  + +Q+  L  G+EIVIATPGRL+D+LE     L + TY+++DE DRM+DM FE  +RKI+  +     +PD                    RQT+M++AT    V++LA  +L  P  V +GS+    +    Q++ ++++ +KRD+L    E  S++     +IF + K+  D + K L +    A++ HG K Q  R++ +   ++    I+VAT++A RGID+K ++ V+NYDMP  I++Y+HR+GRTGRAG  G AISF T+ +  L
Sbjct:   95 FRKENEMTISGHDIPKPITTFDEAGFPDYVLNEVKAEG-FDKPTGIQCQGWPMALSGRDMVGIAATGSGKTLSYCLPGIVHINAQPLLAPGD----GPIVLVLAPTRELAVQIQTECSKFGHSSRIRNTCVYGGVPKSQQIRDLSRGSEIVIATPGRLIDMLEIGKTNLKRVTYLVLDEADRMLDMGFEPQIRKIVDQI-----RPD--------------------RQTLMWSATWPKEVKQLAADYLNDPIQVQVGSLELSASHNITQIVEVVSDFEKRDRLNKYLETASQDNEYKTLIFASTKRMCDDITKYLREDGWPALAIHGDKDQRERDWVLQEFRNGRSPIMVATDVAARGIDVKGINYVINYDMPGNIEDYVHRIGRTGRAGATGTAISFFTEQNKGL 471

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Yeast
Match: DED1 (ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase; required for translation initiation of all yeast mRNAs; binds to mRNA cap-associated factors, and binding stimulates Ded1p RNA-dependent ATPase activity; mutation in human homolog DBY is associated with male infertility; human homolog DDX3X complements ded1 null mutation; DED1 has a paralog, DBP1, that arose from the whole genome duplication [Source:SGD;Acc:S000005730])

HSP 1 Score: 262.307 bits (669), Expect = 1.168e-75
Identity = 148/405 (36.54%), Postives = 222/405 (54.81%), Query Frame = 1

Query: 1066 EDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKL-PRLQKMEQNELG-----PYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDS 2262
            +D  +   G ++P  I  +    L D LL   I  A++ +PTP+Q+ ++PI    RD++  A+TGSGKT  FL P+LS   K  P  Q   Q         P A+I+ PTR+LA QI +E  KF     +KA  + GG+    Q+ ++  G ++++ATPGRL D+LE   I L    Y+++DE DRM+DM FE  +R I+               ED +       T    RQT+MF+AT    ++ LAR FL     + +G +G  +E   Q +  +    K+  L +++S +     +IFV  K+ AD L   L   N +A + HG + Q  RE A+AA +S    +LVAT +A RG+DI +V+ V+NYD+P  +D+Y+HR+GRTGRAG  GLA +F   ++S
Sbjct:  126 DDIPVDASGKDVPEPITEFTSPPL-DGLLLENIKLARFTKPTPVQKYSVPIVANGRDLMACAQTGSGKTGGFLFPVLSESFKTGPSPQPESQGSFYQRKAYPTAVIMAPTRELATQIFDEAKKFTYRSWVKACVVYGGSPIGNQLREIERGCDLLVATPGRLNDLLERGKISLANVKYLVLDEADRMLDMGFEPQIRHIV---------------EDCDM------TPVGERQTLMFSATFPADIQHLARDFLSDYIFLSVGRVGSTSENITQKVLYVENQDKKSALLDLLSASTDGLTLIFVETKRMADQLTDFLIMQNFRATAIHGDRTQSERERALAAFRSGAATLLVATAVAARGLDIPNVTHVINYDLPSDVDDYVHRIGRTGRAGNTGLATAFFNSENS 508

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Yeast
Match: DBP1 (Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress; DBP1 has a paralog, DED1, that arose from the whole genome duplication [Source:SGD;Acc:S000006040])

HSP 1 Score: 259.225 bits (661), Expect = 1.600e-74
Identity = 145/404 (35.89%), Postives = 223/404 (55.20%), Query Frame = 1

Query: 1066 EDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWI-----EKLP-RLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDD 2259
            ++  +   G ++P  I  +    L D+LL   I  A + +PTP+Q+ +IPI  + RD++  A+TGSGKT  FL PL + +       +P + Q     +  P A++L PTR+LA QI EE  KF     ++   + GG     Q+ ++  G ++++ATPGRL D+LE   + L    Y+++DE DRM+DM FE  +R I+    E  + P  ++                 RQT+MF+AT    ++ LAR FL     + +G +G  +E   Q I  + +  K+  L +++S       +IFV  K+ AD L   L   N KA + HG + Q  RE A++A K+   DILVAT +A RG+DI +V+ V+NYD+P  ID+Y+HR+GRTGRAG  G+A SF   ++
Sbjct:  138 DNIPVDASGKDVPEPILDFSSPPL-DELLMENIKLASFTKPTPVQKYSIPIVTKGRDLMACAQTGSGKTGGFLFPLFTELFRSGPSPVPEKAQSFYSRKGYPSALVLAPTRELATQIFEEARKFTYRSWVRPCVVYGGAPIGNQMREVDRGCDLLVATPGRLNDLLERGKVSLANIKYLVLDEADRMLDMGFEPQIRHIV----EECDMPSVEN-----------------RQTLMFSATFPVDIQHLARDFLDNYIFLSVGRVGSTSENITQRILYVDDMDKKSALLDLLSAEHKGLTLIFVETKRMADQLTDFLIMQNFKATAIHGDRTQAERERALSAFKANVADILVATAVAARGLDIPNVTHVINYDLPSDIDDYVHRIGRTGRAGNTGVATSFFNSNN 519

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Yeast
Match: RRP3 (Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity [Source:SGD;Acc:S000001107])

HSP 1 Score: 221.09 bits (562), Expect = 1.140e-62
Identity = 137/391 (35.04%), Postives = 205/391 (52.43%), Query Frame = 1

Query: 1108 SIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLS--WIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLEN-RYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLF 2271
            S   + E  L  +L+    N   Y +PTPIQ +AIP  L+  DIIG+A+TGSGKTAAF IP+L+  W ++ P            YA IL PTR+LA QI E     G  +G+++  I+GG +  +Q   L     I+IATPGRL+D LEN +   L +  +++MDE DR++DM F   + +IL  +P                       TQ+ R T +F+ATM   +++L R  L  P    + +  +  +   Q + ++    K   L  +++      +IIF   K  A+ L+     +   A + HG   Q  R  ++   K+ ++ ILVAT++A RG+DI  V +V+NYD+P     YIHRVGRT RAG+ G +IS V++ D  L 
Sbjct:   79 SFESFSELNLVPELIQACKN-LNYSKPTPIQSKAIPPALEGHDIIGLAQTGSGKTAAFAIPILNRLWHDQEPY-----------YACILAPTRELAQQIKETFDSLGSLMGVRSTCIVGGMNMMDQARDLMRKPHIIIATPGRLMDHLENTKGFSLRKLKFLVMDEADRLLDMEFGPVLDRILKIIP-----------------------TQE-RTTYLFSATMTSKIDKLQRASLTNPVKCAVSNKYQTVDTLVQTLMVVPGGLKNTYLIYLLNEFIGKTMIIFTRTKANAERLSGLCNLLEFSATALHGDLNQNQRMGSLDLFKAGKRSILVATDVAARGLDIPSVDIVVNYDIPVDSKSYIHRVGRTARAGRSGKSISLVSQYDLELI 433

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Nematostella
Match: EDO43602 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYA5])

HSP 1 Score: 731.48 bits (1887), Expect = 0.000e+0
Identity = 362/570 (63.51%), Postives = 458/570 (80.35%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLYI 2406
            RYLG  +  ++ +R   +RKFVF WD GEDTS DYNP+YK++H+ Q FGRGHIGGIDI  QKK  G+FY +MM  RR+  +K+QE  RLK   ++E KV +D+R WT+K+L+EMT RDWRIFREDF+I+TKGG IP  IR W+EA + D +L+ I++K  YK+PTPIQRQAIPIGLQNRDIIGVAETGSGKTAAF IPLL WI  LP++++    + GPYA+IL PTR+LA QI EE +KFG+PLGI+ V++IGG SRE+Q FQLRLG EIVIATPGRL+DVLENRY+VLN+C+Y++MDE DRMIDM FE +V+KIL +LP +  KPD++D+ED E LL +     KYRQTVMFTATM P VERLA+ +LRRPA VYIGS+GKP ER +Q +Y++ E QKR KL E+++++  PPIIIFVNQKKGADVLAK LEKM  +A + HGG+ QE RE+A+++LK   KDILVAT++AGRGIDIKDVS V+NYDM KTI++Y HR+GRTGRAGK G+A+SF+T+ DS +FYDLK+LL  SP+S  PSEL +HP+AQ K GT  +KKR+ EET+++
Sbjct:  212 RYLGLNKKKRKIRRL-TDRKFVFDWDTGEDTSNDYNPIYKERHQVQLFGRGHIGGIDIKIQKKEAGKFYAEMMEKRRTIEEKEQEGNRLKKEKDKERKVAFDDRHWTKKNLEEMTERDWRIFREDFNISTKGGRIPFPIRKWKEAQIPDSILE-IVDKLGYKDPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFAIPLLVWIMGLPKIERDNDADQGPYALILAPTRELAQQIEEEILKFGRPLGIRTVSVIGGLSREDQGFQLRLGVEIVIATPGRLIDVLENRYLVLNRCSYIVMDEADRMIDMGFEPEVQKILEHLPVSNVKPDSEDSEDPEHLLTHM-GKDKYRQTVMFTATMPPQVERLAKNYLRRPAVVYIGSVGKPVERVEQRVYLVNEQQKRKKLLELLNKDLEPPIIIFVNQKKGADVLAKSLEKMGFRATTLHGGRNQEQREFALSSLKGGSKDILVATDVAGRGIDIKDVSHVINYDMAKTIEDYTHRIGRTGRAGKTGIAVSFLTQSDSGVFYDLKQLLLSSPVSSCPSELANHPEAQHKPGTVLTKKRK-EETIFV 777

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Nematostella
Match: EDO42894 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S034])

HSP 1 Score: 287.345 bits (734), Expect = 4.590e-82
Identity = 159/422 (37.68%), Postives = 241/422 (57.11%), Query Frame = 1

Query: 1027 LDEMTNRDWRIFREDF-SITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVL---ENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQK-RDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYD 2277
            L +MT  +   FR    +I  +G N P  ++ W + G+  K+LDV+  K  Y++PTPIQ QAIP+ +  RD+IG+A+TGSGKT AFLIP+   I+  P L++ E    GP AI++ PTR+LAIQI  E  KF +P  ++ V + GG    EQ+ +L+ GAEI++ TPGR++D+L     R     +CTY+++DE DRM DM FE  V +I+  +     +PD                    RQTVMF+AT    +E LAR  L +P  + +G         +Q + ++ E+ K    L  +        +++FV ++  AD L K L K +   +S HGG  Q  R+  +A  K+    +++AT++A RG+D+K ++LV+NYD P   ++Y+HRVGRTGRAG +G A +F+T +      D
Sbjct:  107 LAKMTPEETDEFRLSLENIHVRGKNAPKPVKTWAQTGVQLKILDVL-KKNSYEKPTPIQAQAIPVIMSGRDMIGIAKTGSGKTLAFLIPMFRHIKDQPPLEREE----GPIAIVMTPTRELAIQIHRECKKFCKPNNLRCVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLTANNGRVTNCQRCTYLVLDEADRMFDMGFEPQVMRIIDCI-----RPD--------------------RQTVMFSATFPRQMEALARKILDKPIEIQVGGRSVVCSDVEQNVVVIEEDDKFLKLLELLGLYQEQGSVLVFVEKQDSADSLFKDLLKRSYPCLSLHGGMDQFDRDSTIADFKNGVTKLMIATSVAARGLDVKHLNLVVNYDCPNHYEDYVHRVGRTGRAGNKGTAYTFLTPEQGRFAID 498

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Nematostella
Match: EDO49247 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGX3])

HSP 1 Score: 266.544 bits (680), Expect = 5.668e-78
Identity = 147/421 (34.92%), Postives = 233/421 (55.34%), Query Frame = 1

Query: 1012 WTE-KHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPL------GIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTK 2253
            WT  +++  M        R+ + I  +G +IP  ++ ++E      +L   + K     PTPIQ Q +P  L  RD+IG+A TGSGKT  F +P++ +  +  +    ++NE GPY +I+VP+R+LA Q  E    F + L       ++    IGG+S +EQ   ++ G  +V+ATPGRL+D+L+ R I L+ C Y+++DE DRMIDM FE DVR I SY                          +  RQT++F+ATM   ++  A++ L +P TV +G  G  +    Q +  + +  K   L E + +   PP++IF  +K   D + + L    ++A++ HG K QE R +A+       KD+LVAT++A +G+D  D+  V+N+DMP+ I+ Y+HR+GRTGR GK G+A +F+ K
Sbjct:   12 WTPPRYILHMPKEKIERIRKKWHILVEGDDIPPPVKTFKEMKFPRPIL-AALKKKGITHPTPIQVQGLPAVLTGRDMIGIAFTGSGKTLVFTLPIIMFSLEQEKAMPFQRNE-GPYGMIVVPSRELARQTFEVITHFSRALEAHGFPSLRTNLCIGGSSIKEQSDAMKRGVHMVVATPGRLMDLLDKRIITLDVCRYLVLDEADRMIDMGFEEDVRTIFSYF-------------------------KSQRQTLLFSATMPKKIQNFAKSALVKPVTVNVGRAGAASLDVIQEVEYVKQEAKVVYLLECLQKT-PPPVLIFAEKKSDVDDIHEYLLLKGVEAVAIHGDKSQEERVHAIREFHQGNKDVLVATDVASKGLDFPDIQHVINFDMPEDIENYVHRIGRTGRCGKTGVATTFINK 404

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Nematostella
Match: EDO43557 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RY08])

HSP 1 Score: 254.988 bits (650), Expect = 1.385e-73
Identity = 149/405 (36.79%), Postives = 218/405 (53.83%), Query Frame = 1

Query: 1060 FREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVP-----PIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDD 2259
             R+   I   G         +   G  ++++   I K +Y +PT IQ QA+PI L  RDIIG+A+TGSGKTAAFL P L  I   P LQ  +    GP  +I  PTR+L  QI  E  +FG+   I  V + GG ++ EQ   L+ GAEIV+ATPGRL+D ++ +   L++ TY++ DE DRM DM FE  VR I + +     +PD                    RQT++F+AT +  VE L R  L  P  V IG +G+  E   Q++++      +    E +++N V       ++IFV +K  ++ LA  L K + +    HG   Q  R   +   K +E  ILVAT++A RG+DI  +  V+NYD+ + I  + HR+GRTGRAG++G A + +T+ D
Sbjct:   88 LRKKMGIKVSGAMPARPCISFAHFGFDEQMM-ASIRKLEYTQPTQIQCQALPIALSGRDIIGIAKTGSGKTAAFLWPALVHIMDQPELQVGD----GPIVLICAPTRELCQQIYTEARRFGKAYNIHVVAVFGGGNKYEQSKALQEGAEIVVATPGRLIDHVKAKATNLHRVTYLVFDEADRMFDMGFEPQVRSIANNV-----RPD--------------------RQTLLFSATFKKKVEHLCRDILVDPVRVVIGELGEANEDVTQIVHIFNSMPSK---WEWLTQNLVSFASAGSVLIFVTKKLNSEELATNLRKNDFEVALLHGDMDQFERSKVLGQFKKREIPILVATDVAARGLDIPSIKTVINYDVARDITTHTHRIGRTGRAGEKGNAYTLLTQSD 459

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Nematostella
Match: EDO38061 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDX5])

HSP 1 Score: 251.136 bits (640), Expect = 4.322e-73
Identity = 137/406 (33.74%), Postives = 220/406 (54.19%), Query Frame = 1

Query: 1060 FREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIG-KPTERTKQVIYMLTENQKRDK----LREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDS 2262
            +R    +T  G N+P  +  +EE+   D  +     +  + EPT IQ Q  P+ L  R+++G+A+TGSGKT +F++P +  I   P LQ  +    GP  ++L PTR+LA Q+ E     G+   +++  I GG  +  Q+ +L  G EI IATPGRL+D+LE+R   L +CTY+++DE DRM+DM FE  +R I+  +     +PD                    RQT+M++AT    V+ LA  FL     + +GS+G     +  Q++ +  +++K  K    L EIM        +IF   K+ AD L + L      A+  HG K Q  R++ ++  +     ILVAT++A RG+DI D+  V+N+D P   ++Y+HR+GRT R+ + G + +F T +++
Sbjct:   40 YRRSKDLTVNGRNVPKPVTTFEESAFPD-YIQSYFKREGFTEPTMIQAQGWPVALTGRNLVGIAQTGSGKTLSFILPGIVHINHQPLLQPGD----GPIVLVLCPTRELAQQVQEVAYSVGKHCKLRSTCIYGGAPKGPQIRELERGVEICIATPGRLIDMLESRKTNLRRCTYLVLDEADRMLDMGFEPQIRTIIDQI-----RPD--------------------RQTLMWSATWPKEVQGLAHDFLSDYVHITVGSLGLTANHKILQIVDVCEDHEKEHKLLKLLEEIMGEK-ENKTLIFTETKRRADELTRKLRSDGWPAMCIHGDKAQPERDWVLSEFRKGHAPILVATDVASRGLDISDIKFVINFDFPNCTEDYVHRIGRTARSDRTGTSYTFFTVNNA 414

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Medaka
Match: ddx23 (DEAD-box helicase 23 [Source:NCBI gene;Acc:101157453])

HSP 1 Score: 741.88 bits (1914), Expect = 0.000e+0
Identity = 363/570 (63.68%), Postives = 454/570 (79.65%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            RYLG  +  +R +   N+RKFVF WD  EDTS DYNP+YK+KH+ Q +GRG I GID+  QK+   +FY  +M  RR+  +K+QE  RLK +  +EAK +WD+R W++K LDEMT+RDWRIFRED+SITTKGG IPN IR W+E  L   +L+VI +K  YKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL WI  LP++ ++E ++ GPYA+IL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G EIVIATPGRL+DVLENRY+VL +CTYV++DE DRMIDM FE DV+KIL Y+P   +KPDTD+AED EK+  NF +   KYRQTVMFTATM P+VERLAR++LRR A VYIGS GKP ER +Q + +++E +KR K+ E+++  F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI DVS+V+NYDM K I++YIHR+GRTGRAGK G+A++F+TK+DS +FYDLK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:  235 RYLGGIKKRRRTRHL-NDRKFVFEWDASEDTSVDYNPIYKEKHQVQLYGRGFIAGIDLKQQKRDQSRFYGDLMEKRRTLEEKEQEETRLKKVRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRNWKEYALPAHILEVI-DKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEDSDQGPYAVILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFKLRMGCEIVIATPGRLIDVLENRYLVLGRCTYVVLDEADRMIDMGFEPDVQKILEYIPVTNQKPDTDEAEDPEKMTMNFESGKHKYRQTVMFTATMPPAVERLARSYLRRAAVVYIGSAGKPHERVEQKVMLMSEGEKRKKMLEVLASGFEPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIHDVSMVINYDMAKNIEDYIHRIGRTGRAGKSGVAMTFLTKEDSAVFYDLKQAILESPVSNCPPELANHPDAQHKPGTILTKKRR-EETIF 801

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Medaka
Match: ddx23 (DEAD-box helicase 23 [Source:NCBI gene;Acc:101157453])

HSP 1 Score: 740.339 bits (1910), Expect = 0.000e+0
Identity = 363/570 (63.68%), Postives = 454/570 (79.65%), Query Frame = 1

Query:  697 RYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHT-TQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLY 2403
            RYLG  +  +R +   N+RKFVF WD  EDTS DYNP+YK+KH+ Q +GRG I GID+  QK+   +FY  +M  RR+  +K+QE  RLK +  +EAK +WD+R W++K LDEMT+RDWRIFRED+SITTKGG IPN IR W+E  L   +L+VI +K  YKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLL WI  LP++ ++E ++ GPYA+IL PTR+LA QI EET+KFG+PLGI+ V +IGG SRE+Q F+LR+G EIVIATPGRL+DVLENRY+VL +CTYV++DE DRMIDM FE DV+KIL Y+P   +KPDTD+AED EK+  NF +   KYRQTVMFTATM P+VERLAR++LRR A VYIGS GKP ER +Q + +++E +KR K+ E+++  F PPIIIFVNQKKG DVLAK LEKM   A + HGGKGQE RE+A++ LK+  KDILVAT++AGRGIDI DVS+V+NYDM K I++YIHR+GRTGRAGK G+A++F+TK+DS +FYDLK+ + ESP+S  P EL +HPDAQ K GT  +KKRR EET++
Sbjct:  210 RYLGGIKKRRRTRHL-NDRKFVFEWDASEDTSVDYNPIYKEKHQVQLYGRGFIAGIDLKQQKRDQSRFYGDLMEKRRTLEEKEQEETRLKKVRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRNWKEYALPAHILEVI-DKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEDSDQGPYAVILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFKLRMGCEIVIATPGRLIDVLENRYLVLGRCTYVVLDEADRMIDMGFEPDVQKILEYIPVTNQKPDTDEAEDPEKMTMNFESGKHKYRQTVMFTATMPPAVERLARSYLRRAAVVYIGSAGKPHERVEQKVMLMSEGEKRKKMLEVLASGFEPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIHDVSMVINYDMAKNIEDYIHRIGRTGRAGKSGVAMTFLTKEDSAVFYDLKQAILESPVSNCPPELANHPDAQHKPGTILTKKRR-EETIF 776

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Medaka
Match: ddx46 (DEAD-box helicase 46 [Source:NCBI gene;Acc:101161571])

HSP 1 Score: 286.574 bits (732), Expect = 3.617e-80
Identity = 161/416 (38.70%), Postives = 241/416 (57.93%), Query Frame = 1

Query: 1024 HLDEMTNRDWRIFREDF-SITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVL---ENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSR-NFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKD 2256
             L +MT  +   +R +   I  KG   P  I+ W + G+S K+L  +  K  Y +PTPIQ QAIP  +  RD+IG+A+TGSGKT AFL+P+   I     L++ E    GP ++I+ PTR+LA+QI +E  KF + LG++ V + GG    EQ+ +L+ GAEI++ TPGR++D+L     R   L + TYV++DE DRM DM FE  V +I+  +     +PD                    RQTVMF+AT   ++E LAR  L +P  V +G         +Q + ++ E+QK  KL EI+        +IIFV++++ AD L K L K +   +S HGG  Q  R+  +   K+    ++VAT++A RG+D+K + LV+NY+ P   ++Y+HR GRTGRAG +G A +F+T+D
Sbjct:  246 ELAKMTTEEVNAYRLELEGIMVKGKGCPKPIKTWVQCGVSMKILSAL-KKQGYDKPTPIQAQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRPLEESE----GPISVIMTPTRELALQITKECKKFSKALGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLGANSGRVTNLRRATYVVLDEADRMFDMGFEPQVMRIVDNV-----RPD--------------------RQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQHVLVIEEDQKFLKLLEILGHYQEKGSVIIFVDKQEHADGLLKDLMKASYPCLSLHGGIDQYDRDSIINDFKNGVCRLMVATSVAARGLDVKQLILVVNYNCPNHYEDYVHRAGRTGRAGNKGYAYTFITED 631

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Medaka
Match: ddx46 (DEAD-box helicase 46 [Source:NCBI gene;Acc:101161571])

HSP 1 Score: 286.96 bits (733), Expect = 1.172e-79
Identity = 161/416 (38.70%), Postives = 241/416 (57.93%), Query Frame = 1

Query: 1024 HLDEMTNRDWRIFREDF-SITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVL---ENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSR-NFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKD 2256
             L +MT  +   +R +   I  KG   P  I+ W + G+S K+L  +  K  Y +PTPIQ QAIP  +  RD+IG+A+TGSGKT AFL+P+   I     L++ E    GP ++I+ PTR+LA+QI +E  KF + LG++ V + GG    EQ+ +L+ GAEI++ TPGR++D+L     R   L + TYV++DE DRM DM FE  V +I+  +     +PD                    RQTVMF+AT   ++E LAR  L +P  V +G         +Q + ++ E+QK  KL EI+        +IIFV++++ AD L K L K +   +S HGG  Q  R+  +   K+    ++VAT++A RG+D+K + LV+NY+ P   ++Y+HR GRTGRAG +G A +F+T+D
Sbjct:  356 ELAKMTTEEVNAYRLELEGIMVKGKGCPKPIKTWVQCGVSMKILSAL-KKQGYDKPTPIQAQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRPLEESE----GPISVIMTPTRELALQITKECKKFSKALGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLGANSGRVTNLRRATYVVLDEADRMFDMGFEPQVMRIVDNV-----RPD--------------------RQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQHVLVIEEDQKFLKLLEILGHYQEKGSVIIFVDKQEHADGLLKDLMKASYPCLSLHGGIDQYDRDSIINDFKNGVCRLMVATSVAARGLDVKQLILVVNYNCPNHYEDYVHRAGRTGRAGNKGYAYTFITED 741

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BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Medaka
Match: ENSORLT00000031911.1 (probable ATP-dependent RNA helicase DDX17 [Source:NCBI gene;Acc:101171584])

HSP 1 Score: 271.166 bits (692), Expect = 1.372e-77
Identity = 152/446 (34.08%), Postives = 243/446 (54.48%), Query Frame = 1

Query:  952 AKRLKNISNREAKVKWD-------ERPWTEKH--LDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIG-KPTERTKQVIYMLTENQKRDKLREIMSRNFVP---PIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVT 2250
             K+  N  +R  + KWD       E+ +  +H  ++ M+  D   FR    IT +G   P  +  + +A     ++DV++ +  +KEPT IQ Q  P+ L  RD++G+A+TGSGKT A+L+P +  I   P L + +    GP  ++L PTR+LA Q+ +    +G+   IK+  + GG  +  Q+  L  G EI IATPGRL+D LE+    L +CTY+++DE DRM+DM FE  +RKI+  +     +PD                    RQT+M++AT    V +LA  FL+    + IG++         Q++ + TE++K  KL ++M           IIFV  KK  D L + + +    A+  HG K Q  R++ ++  +S +  +L+AT++A RG+D++DV  V+NYD P + ++YIHR+GRT R+  +G A +F T
Sbjct:   30 GKKFGNPGDRLRRKKWDLDQLPKFEKNFYSEHAEVERMSQFDVEEFRRKKEITVRGSGCPKPLTSFHQAQFPQYVIDVLMQQ-NFKEPTAIQAQGFPLALSGRDMVGIAQTGSGKTLAYLLPAIVHINHQPYLDRGD----GPICLVLAPTRELAQQVQQVACDYGKSSRIKSTCVYGGAPKGPQIRDLERGVEICIATPGRLIDFLESGKTNLRRCTYLVLDEADRMLDMGFEPQIRKIVDQI-----RPD--------------------RQTLMWSATWPKEVRQLAEDFLKEYVQINIGALELSANHNILQIVDVCTESEKDQKLIQLMEEIMAEKENKTIIFVETKKRCDDLTRRMRRDGWPAMCIHGDKSQPERDWVLSEFRSGKAPVLIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTNKGTAYTFFT 445

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Planmine SMEST
Match: SMESG000068237.1 (SMESG000068237.1)

HSP 1 Score: 1314.29 bits (3400), Expect = 0.000e+0
Identity = 793/793 (100.00%), Postives = 793/793 (100.00%), Query Frame = 1

Query:   31 MDDLVNNYQSDQETVDPNQVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNSYNENNEVEFKKLKRGKESFPDIEDFEVEKKIPFNDQSKQNNENAVFKTPNAVPKHAQIPLSVEEVLXXXXXXXXXXXXXXYFSKAEREALAIQRRXXXXXXXXXXXXXXXNLQTKYIEEGRIKXXXXXXXXXXXXXXXXXXXKTAXXXXXXXXXXXXXXXXRYLGXXXXXXXXXXXXNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDXXXXXXXXXXXXXXXXXXXHPDAQQKYGTFTSKKRRNEETLYIA 2409
            MDDLVNNYQSDQETVDPNQVRKSEEIKKSKKKKEKKEKKEKKPRKEKKEKKKHRKRRHENSYNENNEVEFKKLKRGKESFPDIEDFEVEKKIPFNDQSKQNNENAVFKTPNAVPKHAQIPLSVEEVLATKHAEEEAEKKPKYFSKAEREALAIQRREEKVKESKKKALYEKNLQTKYIEEGRIKSRDGDRRSRRSHGDGRSGDKTAEQNEEDKKREEEAIKERYLGQKRLTKRRQRRLNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDLKELLKESPLSEIPSELLSHPDAQQKYGTFTSKKRRNEETLYIA
Sbjct:    1 MDDLVNNYQSDQETVDPNQVRKSEEIKKSKKKKEKKEKKEKKPRKEKKEKKKHRKRRHENSYNENNEVEFKKLKRGKESFPDIEDFEVEKKIPFNDQSKQNNENAVFKTPNAVPKHAQIPLSVEEVLATKHAEEEAEKKPKYFSKAEREALAIQRREEKVKESKKKALYEKNLQTKYIEEGRIKSRDGDRRSRRSHGDGRSGDKTAEQNEEDKKREEEAIKERYLGQKRLTKRRQRRLNERKFVFAWDEGEDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRSDGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDLKELLKESPLSEIPSELLSHPDAQQKYGTFTSKKRRNEETLYIA 793

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Planmine SMEST
Match: SMESG000033908.1 (SMESG000033908.1)

HSP 1 Score: 289.271 bits (739), Expect = 1.539e-84
Identity = 153/396 (38.64%), Postives = 238/396 (60.10%), Query Frame = 1

Query: 1078 ITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIG-KPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDS 2262
            +T KG NI   I  + E  LS +++ VI N  K+ EPTPIQ Q +P+ L  R+++GVA TGSGKTA++LIP +  ++  P L++ +    GP  +I+VPTR+LA Q+      F + +GI++V + GG ++ +Q+ QLR GAEI++ATPGRL+D+L++    L++ TYV++DE DRM+DM FE  +RKI                         F   ++ RQT+M++AT    V+RLA+ FL +   V IGS         +Q++ +L E  K  +L E++S+     +I+F   KK AD++   L     +  + HG K Q  REY +   + +  +ILVAT++A RG+D+ D+ +V+NYD P   ++YIHR+GRTGR GK G++ +F  + +S
Sbjct:  127 MTVKGDNINKPIFSFNEIDLSREIMSVIRNN-KWNEPTPIQAQGLPMALSGRNLVGVARTGSGKTASYLIPAIVHLKAQPPLRRGD----GPICLIVVPTRELAQQVQTVANDFCESVGIRSVCVYGGANKGDQIRQLRSGAEIIVATPGRLIDLLKSSITNLHRTTYVVLDEADRMLDMGFEPQIRKI-------------------------FSQVRQDRQTLMWSATWPKEVQRLAQDFLGKYIQVNIGSQELHANPNIEQIVEILDEYSKDKRLLEVVSKTPRDKMIVFAETKKKADMICSFLRSKQFRVGAMHGDKSQSDREYILNGFRGENLNILVATDVASRGLDVDDIKVVINYDFPIQFEDYIHRIGRTGRRGKSGVSYTFFNRKNS 492

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Planmine SMEST
Match: SMESG000033916.1 (SMESG000033916.1)

HSP 1 Score: 288.5 bits (737), Expect = 2.045e-84
Identity = 153/396 (38.64%), Postives = 238/396 (60.10%), Query Frame = 1

Query: 1078 ITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIG-KPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGIDIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDS 2262
            +T KG NI   I  + E  LS +++ VI N  K+ EPTPIQ Q +P+ L  R+++GVA TGSGKTA++LIP +  ++  P L++ +    GP  +I+VPTR+LA Q+      F + +GI++V + GG ++ +Q+ QLR GAEI++ATPGRL+D+L++    L++ TYV++DE DRM+DM FE  +RKI                         F   ++ RQT+M++AT    V+RLA+ FL +   V IGS         +Q++ +L E  K  +L E++S+     +I+F   KK AD++   L     +  + HG K Q  REY +   + +  +ILVAT++A RG+D+ D+ +V+NYD P   ++YIHR+GRTGR GK G++ +F  + +S
Sbjct:  121 MTVKGDNINKPIFSFNEIDLSREIMSVIRNN-KWNEPTPIQAQGLPMALSGRNLVGVARTGSGKTASYLIPAIVHLKAQPPLRRGD----GPICLIVVPTRELAQQVQTVANDFCESVGIRSVCVYGGANKGDQIRQLRNGAEIIVATPGRLIDLLKSSITNLHRTTYVVLDEADRMLDMGFEPQIRKI-------------------------FSQVRQDRQTLMWSATWPKEVQRLAQDFLGKYIQVNIGSQELHANPNIEQIVEILDEYSKDKRLLEVVSKTPRDKMIVFAETKKKADMICSFLRSKQFRVGAMHGDKSQSDREYILNGFRGENLNILVATDVASRGLDVDDIKVVINYDFPIQFEDYIHRIGRTGRRGKSGVSYTFFNRKNS 486

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Planmine SMEST
Match: SMESG000033348.1 (SMESG000033348.1)

HSP 1 Score: 283.878 bits (725), Expect = 3.004e-82
Identity = 154/415 (37.11%), Postives = 241/415 (58.07%), Query Frame = 1

Query: 1078 ITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIG-KPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGI-------------------DIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDS 2262
            +T KG NI   I  ++E  LS +++ VI N  K+ EPTPIQ Q +P+ L  R+++GVA TGSGKTA++LIP +  ++  P L++ +    GP  +I+VPTR+LA Q+      F Q +GI++V + GG ++ +Q+ QLR GAEI++ATPGRL+D+L++    L++ TYV++DE DRM+DM FE  +RKI                         F   ++ RQT+M++AT    V+RLA+ FL +   V IGS         +Q++ ++ E+ K  +L EI+S+N    +I+F   KK AD++   L     +  + HG K Q  R+Y +   + +  +ILVAT++A RG+                   +I D+ +V+NYD P   ++YIHR+GRTGR  K GL+ +F  +++S
Sbjct:  174 MTVKGDNINKPIFSFDEIDLSREIMSVIRNN-KWNEPTPIQAQGLPMALSGRNLVGVARTGSGKTASYLIPAIVHLKAQPPLRRGD----GPICLIVVPTRELAQQVQTVANDFSQSVGIRSVCVYGGANKGDQIRQLRYGAEIIVATPGRLIDLLQSSITNLHRTTYVVLDEADRMLDMGFEPQIRKI-------------------------FSQVRQDRQTLMWSATWPKEVQRLAQDFLGKYIQVNIGSQELHANPNIEQIVEIIDEHAKDKRLLEIVSKNPNDKMIVFAETKKKADIICYFLRSKQFRVGAMHGDKSQSDRDYILKGFRGQHLNILVATDVASRGLVIPNYKWVINLNLLAYRYSNIDDIKIVINYDFPNQFEDYIHRIGRTGRKDKTGLSYTFFNENNS 558

[Close]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Planmine SMEST
Match: SMESG000033348.1 (SMESG000033348.1)

HSP 1 Score: 283.493 bits (724), Expect = 3.409e-82
Identity = 154/415 (37.11%), Postives = 241/415 (58.07%), Query Frame = 1

Query: 1078 ITTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTRDLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGRLVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPDTDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYIGSIG-KPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADVLAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGI-------------------DIKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDS 2262
            +T KG NI   I  ++E  LS +++ VI N  K+ EPTPIQ Q +P+ L  R+++GVA TGSGKTA++LIP +  ++  P L++ +    GP  +I+VPTR+LA Q+      F Q +GI++V + GG ++ +Q+ QLR GAEI++ATPGRL+D+L++    L++ TYV++DE DRM+DM FE  +RKI                         F   ++ RQT+M++AT    V+RLA+ FL +   V IGS         +Q++ ++ E+ K  +L EI+S+N    +I+F   KK AD++   L     +  + HG K Q  R+Y +   + +  +ILVAT++A RG+                   +I D+ +V+NYD P   ++YIHR+GRTGR  K GL+ +F  +++S
Sbjct:  167 MTVKGDNINKPIFSFDEIDLSREIMSVIRNN-KWNEPTPIQAQGLPMALSGRNLVGVARTGSGKTASYLIPAIVHLKAQPPLRRGD----GPICLIVVPTRELAQQVQTVANDFSQSVGIRSVCVYGGANKGDQIRQLRYGAEIIVATPGRLIDLLQSSITNLHRTTYVVLDEADRMLDMGFEPQIRKI-------------------------FSQVRQDRQTLMWSATWPKEVQRLAQDFLGKYIQVNIGSQELHANPNIEQIVEIIDEHAKDKRLLEIVSKNPNDKMIVFAETKKKADIICYFLRSKQFRVGAMHGDKSQSDRDYILKGFRGQHLNILVATDVASRGLVIPNYKWVINLNLLAYRYSNIDDIKIVINYDFPNQFEDYIHRIGRTGRKDKTGLSYTFFNENNS 551

The following BLAST results are available for this feature:

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Human
BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Celegans
BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Fly
BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Zebrafish
BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Xenopus
BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Mouse
BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. UniProt/SwissProt
BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. TrEMBL
BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Cavefish
BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Sea Lamprey
BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Yeast
BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Nematostella
BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Medaka
BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Planmine SMEST

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
DDX23	0.000e+0	63.86	DEAD-box helicase 23 [Source:HGNC Symbol;Acc:HGNC:...	[more]
DDX46	3.119e-77	38.33	DEAD-box helicase 46 [Source:HGNC Symbol;Acc:HGNC:...	[more]
DDX46	3.167e-77	38.33	DEAD-box helicase 46 [Source:HGNC Symbol;Acc:HGNC:...	[more]
DDX43	1.559e-75	36.22	DEAD-box helicase 43 [Source:HGNC Symbol;Acc:HGNC:...	[more]
DDX41	4.663e-75	33.92	DEAD-box helicase 41 [Source:HGNC Symbol;Acc:HGNC:...	[more]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ddx-23	0.000e+0	62.11	DEAD boX helicase homolog [Source:UniProtKB/TrEMB...	[more]
F53H1.1	1.987e-79	37.80	pep chromosome:WBcel235:IV:1297400:1309644:-1 gene...	[more]
F53H1.1	8.221e-78	37.80	pep chromosome:WBcel235:IV:1297222:1309652:-1 gene...	[more]
vbh-1	1.821e-76	36.72	Vasa-and Belle-like Helicase [Source:UniProtKB/Tr...	[more]
vbh-1	1.821e-76	36.72	Vasa-and Belle-like Helicase [Source:UniProtKB/Tr...	[more]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CG10333	0.000e+0	67.61	gene:FBgn0032690 transcript:FBtr0081072	[more]
vas	5.946e-80	39.49	gene:FBgn0283442 transcript:FBtr0445187	[more]
vas	5.946e-80	39.49	gene:FBgn0283442 transcript:FBtr0445185	[more]
vas	5.946e-80	39.49	gene:FBgn0283442 transcript:FBtr0445186	[more]
CG6227	1.076e-79	38.04	gene:FBgn0030631 transcript:FBtr0074011	[more]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ddx23	0.000e+0	64.39	DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 [Source:...	[more]
ddx23	0.000e+0	63.05	DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 [Source:...	[more]
CR936486.1	1.110e-131	66.45	pep chromosome:GRCz11:CHR_ALT_CTG23_2_1:14207403:1...	[more]
ddx46	5.674e-77	39.14	DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 [Source:...	[more]
DDX17	2.948e-76	34.38	si:dkey-156n14.5 [Source:ZFIN;Acc:ZDB-GENE-030131-...	[more]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ankrd9	0.000e+0	63.86	ankyrin repeat domain 9 [Source:Xenbase;Acc:XB-GEN...	[more]
ankrd9	0.000e+0	63.86	ankyrin repeat domain 9 [Source:Xenbase;Acc:XB-GEN...	[more]
ankrd9	0.000e+0	63.86	ankyrin repeat domain 9 [Source:Xenbase;Acc:XB-GEN...	[more]
ankrd9	0.000e+0	63.86	ankyrin repeat domain 9 [Source:Xenbase;Acc:XB-GEN...	[more]
ankrd9	0.000e+0	62.46	ankyrin repeat domain 9 [Source:Xenbase;Acc:XB-GEN...	[more]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Ddx23	0.000e+0	63.86	DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 [Source:...	[more]
Ddx43	5.018e-78	36.81	DEAD (Asp-Glu-Ala-Asp) box polypeptide 43 [Source:...	[more]
Ddx46	2.181e-77	38.33	DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 [Source:...	[more]
Ddx46	2.181e-77	38.33	DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 [Source:...	[more]
Ddx41	8.126e-75	33.92	DEAD (Asp-Glu-Ala-Asp) box polypeptide 41 [Source:...	[more]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q9BUQ8\|DDX23_HUMAN	0.000e+0	63.86	Probable ATP-dependent RNA helicase DDX23 OS=Homo ...	[more]
sp\|Q5RC67\|DDX23_PONAB	0.000e+0	63.51	Probable ATP-dependent RNA helicase DDX23 OS=Pongo...	[more]
sp\|Q5BCU6\|PRP28_EMENI	4.268e-173	50.19	Pre-mRNA-splicing ATP-dependent RNA helicase prp28...	[more]
sp\|Q2UH00\|PRP28_ASPOR	2.518e-172	50.49	Pre-mRNA-splicing ATP-dependent RNA helicase prp28...	[more]
sp\|A1CX72\|PRP28_NEOFI	2.789e-172	50.48	Pre-mRNA-splicing ATP-dependent RNA helicase prp28...	[more]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
G4V9R0	0.000e+0	64.35	Putative dead box ATP-dependent RNA helicase OS=Sc...	[more]
G4V9R1	0.000e+0	64.06	Putative dead box ATP-dependent RNA helicase OS=Sc...	[more]
A0A0R3WBU9	0.000e+0	62.80	Uncharacterized protein OS=Taenia asiatica OX=6051...	[more]
A0A4E0RA85	0.000e+0	61.43	DEAD box ATP dependent RNA helicase OS=Fasciola he...	[more]
A0A2H1CBR0	0.000e+0	61.43	DEAD/DEAH box helicase OS=Fasciola hepatica OX=619...	[more]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ddx23	0.000e+0	64.74	DEAD-box helicase 23 [Source:NCBI gene;Acc:1030374...	[more]
ddx46	3.312e-80	38.90	DEAD-box helicase 46 [Source:NCBI gene;Acc:1030417...	[more]
DDX5	3.594e-75	34.57	probable ATP-dependent RNA helicase DDX5 [Source:N...	[more]
ddx3xb	1.028e-73	35.19	DEAD-box helicase 3 X-linked b [Source:ZFIN;Acc:ZD...	[more]
DDX41	1.306e-73	36.14	DEAD-box helicase 41 [Source:NCBI gene;Acc:1030356...	[more]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ddx23	0.000e+0	57.77	DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 [Source:...	[more]
ddx46	1.178e-77	36.87	DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 [Source:...	[more]
ENSPMAT00000003898.1	1.448e-74	35.71	pep scaffold:Pmarinus_7.0:GL481305:13824:21767:-1 ...	[more]
ddx42	8.798e-71	37.59	DEAD (Asp-Glu-Ala-Asp) box helicase 42 [Source:ZFI...	[more]
ENSPMAT00000000216.1	1.332e-67	36.61	pep scaffold:Pmarinus_7.0:GL476721:117971:126978:-...	[more]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5


Match Name	E-value	Identity	Description
PRP28	2.823e-100	38.33	RNA binding protein; involved in RNA isomerization...	[more]
DBP2	1.699e-79	36.86	ATP-dependent RNA helicase of the DEAD-box protein...	[more]
DED1	1.168e-75	36.54	ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helic...	[more]
DBP1	1.600e-74	35.89	Putative ATP-dependent RNA helicase of the DEAD-bo...	[more]
RRP3	1.140e-62	35.04	Protein involved in rRNA processing; required for ...	[more]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO43602	0.000e+0	63.51	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42894	4.590e-82	37.68	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO49247	5.668e-78	34.92	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO43557	1.385e-73	36.79	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO38061	4.322e-73	33.74	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ddx23	0.000e+0	63.68	DEAD-box helicase 23 [Source:NCBI gene;Acc:1011574...	[more]
ddx23	0.000e+0	63.68	DEAD-box helicase 23 [Source:NCBI gene;Acc:1011574...	[more]
ddx46	3.617e-80	38.70	DEAD-box helicase 46 [Source:NCBI gene;Acc:1011615...	[more]
ddx46	1.172e-79	38.70	DEAD-box helicase 46 [Source:NCBI gene;Acc:1011615...	[more]
ENSORLT00000031911.1	1.372e-77	34.08	probable ATP-dependent RNA helicase DDX17 [Source:...	[more]

BLAST of Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000068237.1	0.000e+0	100.00	SMESG000068237.1	[more]
SMESG000033908.1	1.539e-84	38.64	SMESG000033908.1	[more]
SMESG000033916.1	2.045e-84	38.64	SMESG000033916.1	[more]
SMESG000033348.1	3.004e-82	37.11	SMESG000033348.1	[more]
SMESG000033348.1	3.409e-82	37.11	SMESG000033348.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence
protein sequence

transcript sequence

>SMED30013392 ID=SMED30013392|Name=Pre-mRNA-splicing ATP-dependent RNA helicase PRP28|organism=Schmidtea mediterranea sexual|type=transcript|length=4372bp
TCTTAATACAAAAGTTGTTATTTCAAATAGATGGATGATTTAGTCAATAA
TTATCAATCTGACCAAGAAACTGTTGACCCTAATCAAGTAAGAAAAAGCG
AAGAAATTAAAAAGTCTAAGAAAAAGAAAGAGAAAAAAGAGAAAAAGGAG
AAAAAACCAAGAAAAGAGAAGAAAGAAAAGAAGAAACATAGAAAACGAAG
GCATGAAAATTCCTATAATGAAAACAATGAAGTAGAGTTTAAAAAATTAA
AACGAGGAAAAGAATCTTTCCCGGATATCGAAGATTTTGAGGTAGAAAAG
AAAATTCCGTTTAATGATCAATCTAAGCAAAATAATGAAAATGCAGTTTT
TAAAACGCCTAATGCTGTTCCTAAACATGCACAAATCCCTTTGTCCGTAG
AAGAAGTTTTGGCTACTAAACATGCTGAAGAAGAAGCTGAAAAAAAACCT
AAATATTTTTCAAAAGCTGAGCGAGAAGCACTTGCTATTCAAAGAAGAGA
AGAAAAAGTTAAAGAAAGTAAAAAAAAAGCATTATACGAAAAAAATCTTC
AGACTAAATATATTGAAGAAGGTAGAATTAAAAGTCGTGATGGTGATAGA
AGGTCGAGACGAAGCCATGGCGACGGAAGGAGTGGTGATAAAACCGCCGA
ACAAAATGAAGAAGACAAAAAACGCGAAGAAGAAGCAATAAAAGAACGTT
ATTTAGGGCAAAAAAGGTTGACTAAACGCAGACAGCGTCGATTAAATGAA
CGCAAATTTGTTTTTGCTTGGGATGAAGGGGAAGATACGAGCGCTGATTA
TAATCCATTATACAAGAAGAAACATGAAGCCCAGTTTTTCGGAAGAGGTC
ATATCGGTGGTATTGATATCAATGCACAGAAAAAGGCATTAGGTCAGTTT
TATACAAAAATGATGTCTGATAGACGATCTGATGGACAGAAAGATCAAGA
AGCTAAAAGATTAAAAAACATTTCTAATCGCGAAGCAAAAGTGAAATGGG
ATGAACGACCCTGGACCGAAAAGCATTTGGATGAGATGACAAATAGAGAT
TGGCGTATTTTCCGTGAAGATTTCAGCATTACAACGAAAGGTGGAAACAT
TCCAAACTCGATTAGGTGCTGGGAGGAAGCCGGATTATCGGATAAGCTTC
TCGATGTGATAATTAATAAGGCCAAATACAAAGAACCAACTCCTATTCAA
AGACAAGCCATTCCAATTGGATTACAGAACAGAGATATTATTGGAGTGGC
TGAAACTGGATCCGGAAAAACAGCAGCCTTCCTGATACCGCTGTTGTCTT
GGATTGAAAAATTACCACGATTGCAAAAGATGGAACAAAACGAACTTGGT
CCTTACGCTATAATTCTTGTGCCAACTCGAGATTTAGCCATTCAGATTCT
TGAAGAAACCATGAAATTTGGTCAGCCTCTTGGAATCAAAGCCGTAACAA
TTATCGGTGGTAACAGTCGAGAGGAACAGGTTTTCCAGTTGCGTTTAGGT
GCTGAGATAGTGATTGCAACTCCTGGTCGACTGGTAGATGTATTGGAGAA
CCGATATATCGTACTCAATCAATGTACTTACGTTATCATGGACGAGGTCG
ACAGAATGATCGATATGAATTTCGAGGCGGACGTCAGAAAGATTTTATCT
TATTTGCCCGAGGCTACGGAAAAACCAGATACTGATGATGCCGAAGACAC
TGAAAAACTGTTGCAAAATTTCCATACCACTCAAAAATACAGACAAACTG
TCATGTTTACCGCTACAATGCAACCGTCTGTGGAACGGCTTGCCAGAACA
TTTTTACGTCGCCCGGCAACGGTCTATATTGGTTCTATCGGCAAGCCAAC
TGAACGCACCAAACAAGTGATCTACATGCTGACCGAAAACCAAAAACGTG
ACAAACTACGGGAAATTATGAGCAGGAATTTCGTCCCGCCGATTATCATC
TTTGTCAATCAAAAGAAAGGAGCCGATGTTTTGGCAAAGGGTCTCGAGAA
AATGAATTTGAAAGCCATCAGTTTCCACGGAGGAAAGGGGCAGGAACACA
GAGAATATGCAATGGCTGCCCTCAAATCCAAGGAGAAGGATATTTTAGTA
GCTACTAATTTAGCTGGTCGCGGTATTGATATCAAGGATGTTTCACTTGT
TCTTAATTACGACATGCCTAAAACTATTGACGAATATATTCATCGGGTTG
GTAGGACTGGTCGTGCTGGCAAGGAAGGCTTGGCAATCAGTTTCGTTACT
AAGGACGATTCGCCTTTGTTTTATGATTTAAAAGAGCTTTTGAAAGAATC
TCCACTCAGTGAAATCCCCAGTGAACTTTTAAGTCATCCCGATGCTCAAC
AGAAATATGGGACATTTACATCGAAAAAGCGAAGAAATGAGGAAACTCTG
TACATAGCTTAATATGTCGTTCAATAAAATATTCATGTCTCGTTATGCGG
GCTCGATTAATAATTTTCAAATAATAAAGGAAATTTAACTTACTTATAAT
TTGTCAGTAATGTATGTTTTAGATAATTAACCTTTTGATGTCCAGCTGAA
ATTATCTCGAATTTCAGAAGATATAGAAAAGGAGAATTCATTAAGAACTT
GTTTAATATATTAACAGTAAAATACTCCCTTGTGCCATTATATCCTTTTT
GTGGAAACCTCCATTTCATTTAGGGATAATTTCAAAAAGTTTACACTAAA
AATTTTGATATTTAGGTAATCTATTTTAATTGGGTGCATATTTAAAACTT
ATTTCGTCTAAAAATCGCATCAGAAGGAAAATTACAATATATTTAACAAC
ACAACTAACTAATAATCGTTCATTAAAAATGTGATTTTCGTGCTGCCAAT
GTCTAAATTAAGTTTCAATAAATTTGTCTTTGTAAATGCGGATTGAAATC
TCGACGTATCATTGGTCATTGTTATGTTCTTAAATAATATACAAGAATAA
AAATTTACCTCATGCAAAAAATCAAATTTGGAGTGTTTTTAGTATGACTG
TTGTACGTGTTTTGTATGCAAAAATATTATATACTGATTGAATGATGTTA
TAATTCGAACTGACAAATTTTTAAGATACCGTATTTCACTCCTTATAAGT
CGCTAGAGACAATGGTGTTGGTTTAACGACTTCAGATACACCGTCATCAG
ATGATTCATTATTCCATTCACTGTCATTTTTAACGATTAAGGAAAATATG
AAAACATTAAATAAAACAAAATTAAAAACCTCGCAGAAAAGTCCACAGCA
GTGGATAGAGACATACTCGAAACTTAAAACCTAGCAAAAGAAGGTTTAGA
AAATACTTATGTGAAGTGGTGAACGAAAATTAAATAATTGAAAGGATCAA
AAAGAAAAATGACAAAAAAATAATAACAAAATAAAAGGTTAAAAAATATA
AATATATCAAAAAGTAAAGAAAATGATATAAAAAATAATATCGTAAAATA
ACTGAAAATTGCCTATTGGTTACACACACACAAAACAACTATAAATACAG
TAGCCAAATTCATAATGTTTATCCTTAAACTAATCAAATTTTGACTTGAA
TTTGTTGTGAGGAAAAAATTTTTTTGAGTAAGGATGTTTATTTTTATTTG
TAATTGTTGTCTTATATAAAATATATATATTCTTATCTATGAGTTAGTTT
TAATGGATTAACTATTCTGGATGGTCGTGGTGATCAAAGTCAAAGCATTA
CAACAAATCATGAATGAGGAGGGACGCTTTATCTGGCAAGTACATCAAAC
TGCTGCCAGAGACAATTCTATTGTAATCGAAATATCATTGGCTACCCAAA
GATTTCAGAAAGCAATCGCAATGCTTGCTGAAAAGAAAAAAATCCAAAAG
CGTTCCAAAAGAGGTAACTCCAACGGAAATAGCTCCAACAGATCAAACCC
GTATATCCAGACAGCGTTACAATCCAGGAAGCATATCCCAAGCAACACTA
AGATTCCGAAAGTTCTGAGCAGTAGGTACACTAGCCGTGCCTCCTAGAAA
AAAAGCATATCTCAGTAAACAAAGTTGGAGTTTTGAATTTTCTGAATTCG
AGCTCCTAGCGATACAAGAAAAAGAAGGGAAAAGGAAAACTGAACAAAAA
CCTGCAAAGAGCAGAAAAATCAAAATAGTCAAAGAAATGGACGAAATCAA
AAAGAATCATCCAGACGAAGAAATCCAGCAAAAGCATGCCAAAACAAAAC
TAGATATGAAAAATCAAAGATTTTCAAAAAGCCTGGATGCAATCTTTAAA
GAGATATCTTATTATGACTGATAATTTTTTGTAAAAATTTTTCTAAGTTC
AACCCCCCCTACCAAAAATTTTTATGAATTTTATAACAAGCATCTTTTAA
ACTCTGAAAATTGTAAACCGAT

protein sequence of SMED30013392-orf-1

>SMED30013392-orf-1 ID=SMED30013392-orf-1|Name=SMED30013392-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=109bp
MVVVIKVKALQQIMNEEGRFIWQVHQTAARDNSIVIEISLATQRFQKAIA
MLAEKKKIQKRSKRGNSNGNSSNRSNPYIQTALQSRKHIPSNTKIPKVLS
SRYTSRAS*

protein sequence of SMED30013392-orf-2

>SMED30013392-orf-2 ID=SMED30013392-orf-2|Name=SMED30013392-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=794bp
MDDLVNNYQSDQETVDPNQVRKSEEIKKSKKKKEKKEKKEKKPRKEKKEK
KKHRKRRHENSYNENNEVEFKKLKRGKESFPDIEDFEVEKKIPFNDQSKQ
NNENAVFKTPNAVPKHAQIPLSVEEVLATKHAEEEAEKKPKYFSKAEREA
LAIQRREEKVKESKKKALYEKNLQTKYIEEGRIKSRDGDRRSRRSHGDGR
SGDKTAEQNEEDKKREEEAIKERYLGQKRLTKRRQRRLNERKFVFAWDEG
EDTSADYNPLYKKKHEAQFFGRGHIGGIDINAQKKALGQFYTKMMSDRRS
DGQKDQEAKRLKNISNREAKVKWDERPWTEKHLDEMTNRDWRIFREDFSI
TTKGGNIPNSIRCWEEAGLSDKLLDVIINKAKYKEPTPIQRQAIPIGLQN
RDIIGVAETGSGKTAAFLIPLLSWIEKLPRLQKMEQNELGPYAIILVPTR
DLAIQILEETMKFGQPLGIKAVTIIGGNSREEQVFQLRLGAEIVIATPGR
LVDVLENRYIVLNQCTYVIMDEVDRMIDMNFEADVRKILSYLPEATEKPD
TDDAEDTEKLLQNFHTTQKYRQTVMFTATMQPSVERLARTFLRRPATVYI
GSIGKPTERTKQVIYMLTENQKRDKLREIMSRNFVPPIIIFVNQKKGADV
LAKGLEKMNLKAISFHGGKGQEHREYAMAALKSKEKDILVATNLAGRGID
IKDVSLVLNYDMPKTIDEYIHRVGRTGRAGKEGLAISFVTKDDSPLFYDL
KELLKESPLSEIPSELLSHPDAQQKYGTFTSKKRRNEETLYIA*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **molecular function**
Term	Definition
GO:0003724	RNA helicase activity
GO:0003676	nucleic acid binding
GO:0005524	ATP binding
GO:0016787	hydrolase activity
GO:0000166	nucleotide binding
GO:0004004	ATP-dependent RNA helicase activity
GO:0004386	helicase activity

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000099	neuron
PLANA:0000429	neoblast
PLANA:0002109	X1 cell
PLANA:0002111	X2 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR001650	Helicase_C
IPR014001	Helicase_ATP-bd
IPR027417	P-loop_NTPase
IPR011545	DEAD/DEAH_box_helicase_dom
IPR014014	RNA_helicase_DEAD_Q_motif

Vocabulary: **cellular component**
Term	Definition
GO:0071013	catalytic step 2 spliceosome
GO:0005634	nucleus
GO:0005682	U5 snRNP
GO:0005737	cytoplasm

Vocabulary: **biological process**
Term	Definition
GO:0000375	RNA splicing, via transesterification reactions
GO:0006397	mRNA processing
GO:0008150	biological_process
GO:0008380	RNA splicing
GO:0010501	RNA secondary structure unwinding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 21..41
None	No IPR available	GENE3D	G3DSA:3.40.50.300		coord: 603..792 e-value: 7.1E-54 score: 183.9
None	No IPR available	GENE3D	G3DSA:3.40.50.300		coord: 323..602 e-value: 1.8E-88 score: 298.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 180..217
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 59..74
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 32..58
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 17..31
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..74
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..16
None	No IPR available	PANTHER	PTHR24031:SF23	ATP-DEPENDENT RNA HELICASE DDX23-RELATED	coord: 223..742
None	No IPR available	PANTHER	PTHR24031	RNA HELICASE	coord: 223..742
None	No IPR available	CDD	cd18787	SF2_C_DEAD	coord: 611..739 e-value: 2.74424E-52 score: 176.159
None	No IPR available	CDD	cd17945	DEADc_DDX23	coord: 373..599 e-value: 4.10786E-123 score: 366.645
IPR014001	Helicase superfamily 1/2, ATP-binding domain	SMART	SM00487	ultradead3	coord: 382..613 e-value: 1.5E-58 score: 210.5
IPR014001	Helicase superfamily 1/2, ATP-binding domain	PROSITE	PS51192	HELICASE_ATP_BIND_1	coord: 394..598 score: 29.051
IPR001650	Helicase, C-terminal	SMART	SM00490	helicmild6	coord: 649..730 e-value: 1.2E-31 score: 121.2
IPR001650	Helicase, C-terminal	PFAM	PF00271	Helicase_C	coord: 623..730 e-value: 5.4E-26 score: 91.1
IPR001650	Helicase, C-terminal	PROSITE	PS51194	HELICASE_CTER	coord: 609..770 score: 22.544
IPR011545	DEAD/DEAH box helicase domain	PFAM	PF00270	DEAD	coord: 387..586 e-value: 7.1E-49 score: 165.9
IPR014014	RNA helicase, DEAD-box type, Q motif	PROSITE	PS51195	Q_MOTIF	coord: 362..391 score: 9.809
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	SSF52540	P-loop containing nucleoside triphosphate hydrolases	coord: 24..543
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	SSF52540	P-loop containing nucleoside triphosphate hydrolases	coord: 440..750