Nesprin-1

Overview


Name	Nesprin-1
Smed ID	SMED30013390
Length (bp)	1052

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Nesprin-1 (SMED30013390) t-SNE clustered cells

Violin plots show distribution of expression levels for Nesprin-1 (SMED30013390) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Nesprin-1 (SMED30013390) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Nesprin-1 (SMED30013390) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30013390

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30013390	SMESG000069851.1	SmedASXL_007249	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pharynx	SMED30013390	SMESG000069851.1	dd_Smed_v4_10646_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
protonephridia	SMED30013390	SMESG000069851.1	dd_Smed_v4_10646_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30013390	SMESG000069851.1	dd_Smed_v4_10646_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30013390	SMESG000069851.1	dd_Smed_v4_10646_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30013390	SMESG000069851.1	dd_Smed_v4_10646_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30013390	SMESG000069851.1	dd_Smed_v4_10646_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30013390	SMESG000069851.1	Contig32589	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30013390	SMESG000069851.1	Contig32589	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30013390	SMESG000069851.1	Contig40776	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30013390	SMESG000069851.1	Contig40776	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
head region	SMED30013390	SMESG000069851.1	dd_Smed_v6_10646_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
pharynx	SMED30013390	SMESG000069851.1	dd_Smed_v6_10646_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of Nesprin-1 vs. Ensembl Human
Match: SYNE1 (spectrin repeat containing nuclear envelope protein 1 [Source:HGNC Symbol;Acc:HGNC:17089])

HSP 1 Score: 249.21 bits (635), Expect = 7.258e-77
Identity = 135/265 (50.94%), Postives = 178/265 (67.17%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXX--------------------------XXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            +DEQE VQ++TFT WIN++L +  PP  +D+L+E +KDG+ L+ LLE ++G+KL  EQ + M++IH ++NI TAL+FL+ RKIKLVNIN+TDIADGRPSI+LGLMWT+IL+FQIEE                                  +     G  NAK +LL WV  T G +  + V DFG SWR G AF++++H I P LVD   VK ++N+ENL++AF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK YP+
Sbjct:   22 QDEQEIVQKRTFTKWINSHLAKRKPPMVVDDLFEDMKDGVKLLALLEVLSGQKLPCEQGRRMKRIHAVANIGTALKFLEGRKIKLVNINSTDIADGRPSIVLGLMWTIILYFQIEELTSNLPQLQSLSSSASSVDSIVSSETPSPPSKRKVTTKIQG--NAKKALLKWVQYTAGKQTGIEVKDFGKSWRSGVAFHSVIHAIRPELVDLETVKGRSNRENLEDAFTIAETELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHYPD 284

[Close]

BLAST of Nesprin-1 vs. Ensembl Human
Match: SYNE1 (spectrin repeat containing nuclear envelope protein 1 [Source:HGNC Symbol;Acc:HGNC:17089])

HSP 1 Score: 248.825 bits (634), Expect = 1.359e-75
Identity = 135/265 (50.94%), Postives = 178/265 (67.17%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXX--------------------------XXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            +DEQE VQ++TFT WIN++L +  PP  +D+L+E +KDG+ L+ LLE ++G+KL  EQ + M++IH ++NI TAL+FL+ RKIKLVNIN+TDIADGRPSI+LGLMWT+IL+FQIEE                                  +     G  NAK +LL WV  T G +  + V DFG SWR G AF++++H I P LVD   VK ++N+ENL++AF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK YP+
Sbjct:   22 QDEQEIVQKRTFTKWINSHLAKRKPPMVVDDLFEDMKDGVKLLALLEVLSGQKLPCEQGRRMKRIHAVANIGTALKFLEGRKIKLVNINSTDIADGRPSIVLGLMWTIILYFQIEELTSNLPQLQSLSSSASSVDSIVSSETPSPPSKRKVTTKIQG--NAKKALLKWVQYTAGKQTGIEVKDFGKSWRSGVAFHSVIHAIRPELVDLETVKGRSNRENLEDAFTIAETELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHYPD 284

[Close]

BLAST of Nesprin-1 vs. Ensembl Human
Match: SYNE1 (spectrin repeat containing nuclear envelope protein 1 [Source:HGNC Symbol;Acc:HGNC:17089])

HSP 1 Score: 241.506 bits (615), Expect = 2.478e-75
Identity = 129/272 (47.43%), Postives = 171/272 (62.87%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRK-------IKLVNINATDIADGRPSIILGLMWTV--------------------------XXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            +DEQE VQ++TFT WIN++L +  PP  +D+L+E +KDG+ L+ LLE ++G+KL  EQ + M++IH ++NI TAL+FL+ RK       IKLVNIN+TDIADGRPSI+LGLMWT+                                E         +     G  NAK +LL WV  T G +  + V DFG SWR G AF++++H I P LVD   VK ++N+ENL++AF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK YP+
Sbjct:    5 QDEQEIVQKRTFTKWINSHLAKRKPPMVVDDLFEDMKDGVKLLALLEVLSGQKLPCEQGRRMKRIHAVANIGTALKFLEGRKSMHRGSPIKLVNINSTDIADGRPSIVLGLMWTIILYFQIEELTSNLPQLQSLSSSASSVDSIVSSETPSPPSKRKVTTKIQG--NAKKALLKWVQYTAGKQTGIEVKDFGKSWRSGVAFHSVIHAIRPELVDLETVKGRSNRENLEDAFTIAETELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHYPD 274

[Close]

BLAST of Nesprin-1 vs. Ensembl Human
Match: SYNE1 (spectrin repeat containing nuclear envelope protein 1 [Source:HGNC Symbol;Acc:HGNC:17089])

HSP 1 Score: 252.677 bits (644), Expect = 1.252e-74
Identity = 135/265 (50.94%), Postives = 178/265 (67.17%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXX--------------------------XXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            +DEQE VQ++TFT WIN++L +  PP  +D+L+E +KDG+ L+ LLE ++G+KL  EQ + M++IH ++NI TAL+FL+ RKIKLVNIN+TDIADGRPSI+LGLMWT+IL+FQIEE                                  +     G  NAK +LL WV  T G +  + V DFG SWR G AF++++H I P LVD   VK ++N+ENL++AF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK YP+
Sbjct:   22 QDEQEIVQKRTFTKWINSHLAKRKPPMVVDDLFEDMKDGVKLLALLEVLSGQKLPCEQGRRMKRIHAVANIGTALKFLEGRKIKLVNINSTDIADGRPSIVLGLMWTIILYFQIEELTSNLPQLQSLSSSASSVDSIVSSETPSPPSKRKVTTKIQG--NAKKALLKWVQYTAGKQTGIEVKDFGKSWRSGVAFHSVIHAIRPELVDLETVKGRSNRENLEDAFTIAETELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHYPD 284

[Close]

BLAST of Nesprin-1 vs. Ensembl Human
Match: SYNE1 (spectrin repeat containing nuclear envelope protein 1 [Source:HGNC Symbol;Acc:HGNC:17089])

HSP 1 Score: 252.292 bits (643), Expect = 1.458e-74
Identity = 135/265 (50.94%), Postives = 178/265 (67.17%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXX--------------------------XXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            +DEQE VQ++TFT WIN++L +  PP  +D+L+E +KDG+ L+ LLE ++G+KL  EQ + M++IH ++NI TAL+FL+ RKIKLVNIN+TDIADGRPSI+LGLMWT+IL+FQIEE                                  +     G  NAK +LL WV  T G +  + V DFG SWR G AF++++H I P LVD   VK ++N+ENL++AF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK YP+
Sbjct:    5 QDEQEIVQKRTFTKWINSHLAKRKPPMVVDDLFEDMKDGVKLLALLEVLSGQKLPCEQGRRMKRIHAVANIGTALKFLEGRKIKLVNINSTDIADGRPSIVLGLMWTIILYFQIEELTSNLPQLQSLSSSASSVDSIVSSETPSPPSKRKVTTKIQG--NAKKALLKWVQYTAGKQTGIEVKDFGKSWRSGVAFHSVIHAIRPELVDLETVKGRSNRENLEDAFTIAETELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHYPD 267

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BLAST of Nesprin-1 vs. Ensembl Celegans
Match: unc-70 (Spectrin beta chain [Source:UniProtKB/TrEMBL;Acc:E0AHA7])

HSP 1 Score: 175.252 bits (443), Expect = 2.411e-48
Identity = 103/257 (40.08%), Postives = 153/257 (59.53%), Query Frame = 3

Query:  138 YFEFSTMEDHDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQF 905
            Y +    +D+ S +    + I +  DE+E VQ+KTFT W+N++LV+     K+ +LY  ++DG  L+ LL  ++GE+L K  P  M +IH L N+   L+FL+++ + L N+ + DI DG   + LGL+WT+IL FQI++          AD     +AK +LL W         N+NV +F  SWRDG AFNAL+H   P LVD++ ++      NLQ AF  AE QLG+   LD +DV+V +PDEKSI+TY+  +
Sbjct:   11 YTQDEDYDDNSSARLFERSRIKALADERELVQKKTFTKWVNSHLVRV--SCKVQDLYMDMRDGKMLLRLLAVLSGERLPKPTPGKM-RIHCLENVEKGLQFLRNQHVHLENLGSHDIVDGNSRLTLGLIWTIILRFQIQDITFE-----DADNHETRSAKEALLLWCQMKTAGYPNVNVKNFSTSWRDGLAFNALIHKHRPDLVDYDNLQKSNALYNLQSAFDTAENQLGLAKFLDAEDVNVDQPDEKSIITYVVTY 259

[Close]

BLAST of Nesprin-1 vs. Ensembl Celegans
Match: unc-70 (Spectrin beta chain [Source:UniProtKB/TrEMBL;Acc:E0AHA7])

HSP 1 Score: 175.252 bits (443), Expect = 2.417e-48
Identity = 103/257 (40.08%), Postives = 153/257 (59.53%), Query Frame = 3

Query:  138 YFEFSTMEDHDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQF 905
            Y +    +D+ S +    + I +  DE+E VQ+KTFT W+N++LV+     K+ +LY  ++DG  L+ LL  ++GE+L K  P  M +IH L N+   L+FL+++ + L N+ + DI DG   + LGL+WT+IL FQI++          AD     +AK +LL W         N+NV +F  SWRDG AFNAL+H   P LVD++ ++      NLQ AF  AE QLG+   LD +DV+V +PDEKSI+TY+  +
Sbjct:   11 YTQDEDYDDNSSARLFERSRIKALADERELVQKKTFTKWVNSHLVRV--SCKVQDLYMDMRDGKMLLRLLAVLSGERLPKPTPGKM-RIHCLENVEKGLQFLRNQHVHLENLGSHDIVDGNSRLTLGLIWTIILRFQIQDITFE-----DADNHETRSAKEALLLWCQMKTAGYPNVNVKNFSTSWRDGLAFNALIHKHRPDLVDYDNLQKSNALYNLQSAFDTAENQLGLAKFLDAEDVNVDQPDEKSIITYVVTY 259

[Close]

BLAST of Nesprin-1 vs. Ensembl Celegans
Match: unc-70 (Spectrin beta chain [Source:UniProtKB/TrEMBL;Acc:E0AHA7])

HSP 1 Score: 175.252 bits (443), Expect = 2.708e-48
Identity = 103/257 (40.08%), Postives = 153/257 (59.53%), Query Frame = 3

Query:  138 YFEFSTMEDHDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQF 905
            Y +    +D+ S +    + I +  DE+E VQ+KTFT W+N++LV+     K+ +LY  ++DG  L+ LL  ++GE+L K  P  M +IH L N+   L+FL+++ + L N+ + DI DG   + LGL+WT+IL FQI++          AD     +AK +LL W         N+NV +F  SWRDG AFNAL+H   P LVD++ ++      NLQ AF  AE QLG+   LD +DV+V +PDEKSI+TY+  +
Sbjct:   11 YTQDEDYDDNSSARLFERSRIKALADERELVQKKTFTKWVNSHLVRV--SCKVQDLYMDMRDGKMLLRLLAVLSGERLPKPTPGKM-RIHCLENVEKGLQFLRNQHVHLENLGSHDIVDGNSRLTLGLIWTIILRFQIQDITFE-----DADNHETRSAKEALLLWCQMKTAGYPNVNVKNFSTSWRDGLAFNALIHKHRPDLVDYDNLQKSNALYNLQSAFDTAENQLGLAKFLDAEDVNVDQPDEKSIITYVVTY 259

[Close]

BLAST of Nesprin-1 vs. Ensembl Celegans
Match: unc-70 (Spectrin beta chain [Source:UniProtKB/TrEMBL;Acc:E0AHA7])

HSP 1 Score: 174.866 bits (442), Expect = 3.684e-48
Identity = 99/233 (42.49%), Postives = 144/233 (61.80%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQF 905
            +DE+E VQ+KTFT W+N++LV+     K+ +LY  ++DG  L+ LL  ++GE+L K  P  M +IH L N+   L+FL+++ + L N+ + DI DG   + LGL+WT+IL FQI++          AD     +AK +LL W         N+NV +F  SWRDG AFNAL+H   P LVD++ ++      NLQ AF  AE QLG+   LD +DV+V +PDEKSI+TY+  +
Sbjct:  104 RDERELVQKKTFTKWVNSHLVRV--SCKVQDLYMDMRDGKMLLRLLAVLSGERLPKPTPGKM-RIHCLENVEKGLQFLRNQHVHLENLGSHDIVDGNSRLTLGLIWTIILRFQIQDITFE-----DADNHETRSAKEALLLWCQMKTAGYPNVNVKNFSTSWRDGLAFNALIHKHRPDLVDYDNLQKSNALYNLQSAFDTAENQLGLAKFLDAEDVNVDQPDEKSIITYVVTY 328

[Close]

BLAST of Nesprin-1 vs. Ensembl Celegans
Match: vab-10 (VAB-10B protein [Source:UniProtKB/TrEMBL;Acc:G5EFM4])

HSP 1 Score: 171.785 bits (434), Expect = 3.640e-47
Identity = 93/256 (36.33%), Postives = 140/256 (54.69%), Query Frame = 3

Query:  213 DEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTV-----------------XXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNK--TNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYP 920
            DE++ VQ+KTFT W+N +L  +   +KID+L+  ++DG  LI LLEA+TGE+++KE      + HR+ N+   L+FLK + IKLVNI   DI +G   + LGL+WT+                     Q+  +       +        +A+ +LL W  +       +NV +F  SWRDG AFNA++H    S +DWN + +   +N E L  AF  A+ + G+  LLD +DVD   PDEKSI+TY++    + P
Sbjct:   35 DERDNVQKKTFTKWVNKHL--SKTDHKIDDLFVDLRDGYALIALLEALTGERIQKE--NGYTRFHRIQNVQYCLDFLKKKNIKLVNIRPEDIVEGNGKLTLGLIWTIILNFQVSVIRQRLLLESSQHEQMSAKHTTTNSQVSLHGSDATSARDALLQWARRVTAGYPRVNVNNFSSSWRDGLAFNAILHRYRSSAIDWNKISSDSVSNTERLNNAFAAADREFGVERLLDAEDVDTNNPDEKSIITYVSSLYNALP 286

[Close]

BLAST of Nesprin-1 vs. Ensembl Fly
Match: Msp300 (gene:FBgn0261836 transcript:FBtr0332405)

HSP 1 Score: 229.565 bits (584), Expect = 6.562e-67
Identity = 133/286 (46.50%), Postives = 183/286 (63.99%), Query Frame = 3

Query:  138 YFEF-STMEDHDSVKGKTE--TLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXX-------------XXXGALGADYDGKL-------NAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            Y EF STM   +  +  ++  + I  K++EQERVQ+KTFTNWIN+YL++  PP +ID+L   ++DG  LI LLE ++GE+L  E+ + +++ H LSN NTAL+FL S++IKLVNIN  D+ DGRP ++LGL+WT+IL+FQIEE                  + ++G    G L        A+ +LLNWV   +     + V DFG SWRDG AF AL+  I  +LV+   +K  +N++ L+ AF  AE +LGI  LLD +DVDV KPDEKSIMTY+ QFL  YPE
Sbjct:  598 YQEFQSTMASINYARSNSQYDSHIKEKREEQERVQKKTFTNWINSYLLKRVPPLRIDDLINDLRDGTKLIALLEVLSGERLPVEKGRVLRRPHFLSNANTALQFLASKRIKLVNINPADLVDGRPPVVLGLIWTIILYFQIEENSRNLEYLGHGIGGSVSSLDSVGNQKHGDLKAEKWKQGARKTLLNWVTNALPKDSGVEVKDFGASWRDGVAFLALIDAIKANLVNLAELKKTSNRQRLETAFDVAESKLGIAKLLDAEDVDVPKPDEKSIMTYVAQFLHKYPE 883

[Close]

BLAST of Nesprin-1 vs. Ensembl Fly
Match: Msp300 (gene:FBgn0261836 transcript:FBtr0303385)

HSP 1 Score: 229.565 bits (584), Expect = 6.626e-67
Identity = 133/286 (46.50%), Postives = 183/286 (63.99%), Query Frame = 3

Query:  138 YFEF-STMEDHDSVKGKTE--TLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXX-------------XXXGALGADYDGKL-------NAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            Y EF STM   +  +  ++  + I  K++EQERVQ+KTFTNWIN+YL++  PP +ID+L   ++DG  LI LLE ++GE+L  E+ + +++ H LSN NTAL+FL S++IKLVNIN  D+ DGRP ++LGL+WT+IL+FQIEE                  + ++G    G L        A+ +LLNWV   +     + V DFG SWRDG AF AL+  I  +LV+   +K  +N++ L+ AF  AE +LGI  LLD +DVDV KPDEKSIMTY+ QFL  YPE
Sbjct:  598 YQEFQSTMASINYARSNSQYDSHIKEKREEQERVQKKTFTNWINSYLLKRVPPLRIDDLINDLRDGTKLIALLEVLSGERLPVEKGRVLRRPHFLSNANTALQFLASKRIKLVNINPADLVDGRPPVVLGLIWTIILYFQIEENSRNLEYLGHGIGGSVSSLDSVGNQKHGDLKAEKWKQGARKTLLNWVTNALPKDSGVEVKDFGASWRDGVAFLALIDAIKANLVNLAELKKTSNRQRLETAFDVAESKLGIAKLLDAEDVDVPKPDEKSIMTYVAQFLHKYPE 883

[Close]

BLAST of Nesprin-1 vs. Ensembl Fly
Match: Msp300 (gene:FBgn0261836 transcript:FBtr0303383)

HSP 1 Score: 228.794 bits (582), Expect = 9.907e-67
Identity = 133/286 (46.50%), Postives = 183/286 (63.99%), Query Frame = 3

Query:  138 YFEF-STMEDHDSVKGKTE--TLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXX-------------XXXGALGADYDGKL-------NAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            Y EF STM   +  +  ++  + I  K++EQERVQ+KTFTNWIN+YL++  PP +ID+L   ++DG  LI LLE ++GE+L  E+ + +++ H LSN NTAL+FL S++IKLVNIN  D+ DGRP ++LGL+WT+IL+FQIEE                  + ++G    G L        A+ +LLNWV   +     + V DFG SWRDG AF AL+  I  +LV+   +K  +N++ L+ AF  AE +LGI  LLD +DVDV KPDEKSIMTY+ QFL  YPE
Sbjct:  598 YQEFQSTMASINYARSNSQYDSHIKEKREEQERVQKKTFTNWINSYLLKRVPPLRIDDLINDLRDGTKLIALLEVLSGERLPVEKGRVLRRPHFLSNANTALQFLASKRIKLVNINPADLVDGRPPVVLGLIWTIILYFQIEENSRNLEYLGHGIGGSVSSLDSVGNQKHGDLKAEKWKQGARKTLLNWVTNALPKDSGVEVKDFGASWRDGVAFLALIDAIKANLVNLAELKKTSNRQRLETAFDVAESKLGIAKLLDAEDVDVPKPDEKSIMTYVAQFLHKYPE 883

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BLAST of Nesprin-1 vs. Ensembl Fly
Match: Msp300 (gene:FBgn0261836 transcript:FBtr0332404)

HSP 1 Score: 226.868 bits (577), Expect = 4.307e-66
Identity = 127/263 (48.29%), Postives = 172/263 (65.40%), Query Frame = 3

Query:  198 ILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXX-------------XXXGALGADYDGKLN-------AKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            IL  ++EQERVQ+KTFTNWIN+YL++  PP +ID+L   ++DG  LI LLE ++GE+L  E+ + +++ H LSN NTAL+FL S++IKLVNIN  D+ DGRP ++LGL+WT+IL+FQIEE                  + ++G    G L        A+ +LLNWV   +     + V DFG SWRDG AF AL+  I  +LV+   +K  +N++ L+ AF  AE +LGI  LLD +DVDV KPDEKSIMTY+ QFL  YPE
Sbjct:  262 ILQLQEEQERVQKKTFTNWINSYLLKRVPPLRIDDLINDLRDGTKLIALLEVLSGERLPVEKGRVLRRPHFLSNANTALQFLASKRIKLVNINPADLVDGRPPVVLGLIWTIILYFQIEENSRNLEYLGHGIGGSVSSLDSVGNQKHGDLKAEKWKQGARKTLLNWVTNALPKDSGVEVKDFGASWRDGVAFLALIDAIKANLVNLAELKKTSNRQRLETAFDVAESKLGIAKLLDAEDVDVPKPDEKSIMTYVAQFLHKYPE 524

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BLAST of Nesprin-1 vs. Ensembl Fly
Match: Msp300 (gene:FBgn0261836 transcript:FBtr0303388)

HSP 1 Score: 226.868 bits (577), Expect = 5.071e-66
Identity = 127/263 (48.29%), Postives = 172/263 (65.40%), Query Frame = 3

Query:  198 ILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXX-------------XXXGALGADYDGKLN-------AKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            IL  ++EQERVQ+KTFTNWIN+YL++  PP +ID+L   ++DG  LI LLE ++GE+L  E+ + +++ H LSN NTAL+FL S++IKLVNIN  D+ DGRP ++LGL+WT+IL+FQIEE                  + ++G    G L        A+ +LLNWV   +     + V DFG SWRDG AF AL+  I  +LV+   +K  +N++ L+ AF  AE +LGI  LLD +DVDV KPDEKSIMTY+ QFL  YPE
Sbjct:  262 ILQLQEEQERVQKKTFTNWINSYLLKRVPPLRIDDLINDLRDGTKLIALLEVLSGERLPVEKGRVLRRPHFLSNANTALQFLASKRIKLVNINPADLVDGRPPVVLGLIWTIILYFQIEENSRNLEYLGHGIGGSVSSLDSVGNQKHGDLKAEKWKQGARKTLLNWVTNALPKDSGVEVKDFGASWRDGVAFLALIDAIKANLVNLAELKKTSNRQRLETAFDVAESKLGIAKLLDAEDVDVPKPDEKSIMTYVAQFLHKYPE 524

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BLAST of Nesprin-1 vs. Ensembl Zebrafish
Match: syne1b (spectrin repeat containing, nuclear envelope 1b [Source:ZFIN;Acc:ZDB-GENE-071218-4])

HSP 1 Score: 240.736 bits (613), Expect = 1.003e-70
Identity = 136/270 (50.37%), Postives = 184/270 (68.15%), Query Frame = 3

Query:  195 LILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXG--------------------------ALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            L+   +DEQE VQ++TFT WIN++L +  PP+++++L+E IKDG+ L+ LLE ++G++L  EQ + +++IH +SNI TAL+FL+ RKIKLVNINATDIADGRPSI+LGLMWT+IL+FQIEE    L                             +   + G  NAK +LL WV  T   +  + V DFG SWR G AF++++H I P LVD + V+ ++N+ENL+EAF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK YP+
Sbjct:   17 LMQKLQDEQESVQKRTFTKWINSHLAKHKPPFEVNDLFEDIKDGVKLLALLEVLSGQRLPCEQGRQLKRIHWVSNIGTALKFLEGRKIKLVNINATDIADGRPSIVLGLMWTIILYFQIEELTSNLPALQALSSSASSVDSMASSETGSPPMKRKVVTKFQG--NAKRALLRWVQNTATKRLGIEVKDFGASWRSGVAFHSVIHAIRPELVDMDVVRKRSNRENLEEAFSVAENELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHYPD 284

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Query:  195 LILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXG--------------------------ALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            L+   +DEQE VQ++TFT WIN++L +  PP+++++L+E IKDG+ L+ LLE ++G++L  EQ + +++IH +SNI TAL+FL+ RKIKLVNINATDIADGRPSI+LGLMWT+IL+FQIEE    L                             +   + G  NAK +LL WV  T   +  + V DFG SWR G AF++++H I P LVD + V+ ++N+ENL+EAF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK YP+
Sbjct:   17 LMQKLQDEQESVQKRTFTKWINSHLAKHKPPFEVNDLFEDIKDGVKLLALLEVLSGQRLPCEQGRQLKRIHWVSNIGTALKFLEGRKIKLVNINATDIADGRPSIVLGLMWTIILYFQIEELTSNLPALQALSSSASSVDSMASSETGSPPMKRKVVTKFQG--NAKRALLRWVQNTATKRLGIEVKDFGASWRSGVAFHSVIHAIRPELVDMDVVRKRSNRENLEEAFSVAENELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHYPD 284

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BLAST of Nesprin-1 vs. Ensembl Zebrafish
Match: syne1a (spectrin repeat containing, nuclear envelope 1a [Source:ZFIN;Acc:ZDB-GENE-030131-4172])

HSP 1 Score: 215.312 bits (547), Expect = 6.830e-62
Identity = 129/284 (45.42%), Postives = 177/284 (62.32%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRK-------IKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGA--------------------------DYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYP-----EGGKEIEE 944
            +DEQE VQ++TFT WIN++L + +PP  + +L+E +KDG+ L+ LLE ++G KL  EQ + +++IH ++N+  AL+FL+ R+       IKLVNIN TD+ DGRPSI+LGL+WT+IL+FQIEE    L                                 + G   AK +LL WV  T   +  L+V DFGPSWR G AF+A++  + P LV+   V  + N+ENL+EAF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK +P     E G++ EE
Sbjct:   35 RDEQEAVQKRTFTKWINSHLAKRNPPLVVSDLFEDVKDGVMLLALLEVLSGHKLPCEQGRKLRRIHWVANVGRALKFLEGRRVRQSIPTIKLVNINTTDVVDGRPSIVLGLIWTIILYFQIEELTSHLPALQPQSSSNSSVESSNSTETSSPPVKRKPRLSFQG--GAKRALLKWVQNTATKRMGLDVKDFGPSWRTGVAFHAVIFALRPHLVNMERVWRRGNRENLEEAFSLAERELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHHPDRQHTETGQQQEE 316

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BLAST of Nesprin-1 vs. Ensembl Zebrafish
Match: syne1a (spectrin repeat containing, nuclear envelope 1a [Source:ZFIN;Acc:ZDB-GENE-030131-4172])

HSP 1 Score: 214.927 bits (546), Expect = 9.286e-62
Identity = 129/284 (45.42%), Postives = 177/284 (62.32%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRK-------IKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGA--------------------------DYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYP-----EGGKEIEE 944
            +DEQE VQ++TFT WIN++L + +PP  + +L+E +KDG+ L+ LLE ++G KL  EQ + +++IH ++N+  AL+FL+ R+       IKLVNIN TD+ DGRPSI+LGL+WT+IL+FQIEE    L                                 + G   AK +LL WV  T   +  L+V DFGPSWR G AF+A++  + P LV+   V  + N+ENL+EAF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK +P     E G++ EE
Sbjct:   35 RDEQEAVQKRTFTKWINSHLAKRNPPLVVSDLFEDVKDGVMLLALLEVLSGHKLPCEQGRKLRRIHWVANVGRALKFLEGRRVRQSIPTIKLVNINTTDVVDGRPSIVLGLIWTIILYFQIEELTSHLPALQPQSSSNSSVESSNSTETSSPPVKRKPRLSFQG--GAKRALLKWVQNTATKRMGLDVKDFGPSWRTGVAFHAVIFALRPHLVNMERVWRRGNRENLEEAFSLAERELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHHPDRQHTETGQQQEE 316

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BLAST of Nesprin-1 vs. Ensembl Zebrafish
Match: syne2b (spectrin repeat containing, nuclear envelope 2b [Source:ZFIN;Acc:ZDB-GENE-071218-5])

HSP 1 Score: 190.274 bits (482), Expect = 4.240e-58
Identity = 112/277 (40.43%), Postives = 154/277 (55.60%), Query Frame = 3

Query:  216 EQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXX--------------------------------XXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDK-YNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGKEIEE 944
            EQE++Q++TFTNWIN  L +  PP  + +L+   +DG  L+ LLE M+G+++ K++    Q  HR SNI  AL FLK + IKLVNIN +DI DG+PSIILGL+WT+I+                                              L A +  +++AK +LL WV +        LNV DF  SWR G  F A++H + P++VD    + +TN++NL+EAF  AE +L IP LLD  DVDV  PDEKSIMTY+ QFL+   +     EE
Sbjct:    2 EQEQIQKRTFTNWINAQLSKRSPPVTVVDLFNEFRDGSRLLDLLEVMSGQRIVKKRRGTFQ--HR-SNIEKALAFLKKKSIKLVNINVSDIMDGKPSIILGLVWTIIMHIHIEELASTLSFSSRQSSLESLASLDTRSTNSSARSSPVPPRGSPLHARF--RVSAKKALLLWVREQCQRAGCTLNVKDFKVSWRSGVVFLAILHALRPNIVDLTRARTRTNRQNLEEAFHIAERELHIPRLLDPADVDVRDPDEKSIMTYVAQFLQYSKDASVSDEE 273

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BLAST of Nesprin-1 vs. Ensembl Xenopus
Match: SYNE1 (spectrin repeat containing, nuclear envelope 1 [Source:NCBI gene;Acc:100145637])

HSP 1 Score: 238.424 bits (607), Expect = 9.160e-70
Identity = 132/288 (45.83%), Postives = 179/288 (62.15%), Query Frame = 3

Query:  213 DEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRK--------IKLVNINATDIADGRPSIILGLMWTV------------------XXXXXXXXXXXXXXGALGADYDGKL------NAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWN--GVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE------GGKEIEENIV 953
            DEQE VQ++TFT WIN++L + HPP  I+ L+E IKDG  LI LLE ++G+KL  EQ + +++IH ++NI  AL+FL+ R+        IKLVNIN+TDIADGRPSI+LGL+WT+                  +          +   A       K+      +AK +LL WV  T   +  + V DFGPSWR+G AF+++++ I P LVD +   +K +TN+ENL+EAF  AE +LGIP LLD +DVDV KPDEKSIMTYI QFLK YP+       G+E ++ IV
Sbjct:   31 DEQEAVQKRTFTKWINSHLAKRHPPMVINELFEDIKDGTKLIALLEVLSGQKLPCEQGRQLKRIHWVANIGAALKFLEGRRVMTFLIFQIKLVNINSTDIADGRPSIVLGLIWTIILYFQIEELTSNLPQLHAMSSSTSSVDSVVSSEAASPPIKRKIVNKIQGSAKKALLRWVQYTAAKRLGIEVKDFGPSWRNGIAFHSVIYAIRPDLVDMDIEKLKRRTNRENLEEAFSIAENELGIPRLLDPEDVDVDKPDEKSIMTYIAQFLKHYPDPHHTSLDGQEDKDRIV 318

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BLAST of Nesprin-1 vs. Ensembl Xenopus
Match: SYNE1 (spectrin repeat containing, nuclear envelope 1 [Source:NCBI gene;Acc:100145637])

HSP 1 Score: 220.32 bits (560), Expect = 1.632e-63
Identity = 118/257 (45.91%), Postives = 162/257 (63.04%), Query Frame = 3

Query:  243 FTNWINTYLV---QAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTV------------------XXXXXXXXXXXXXXGALGADYDGKL------NAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWN--GVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            ++  +N Y++   Q HPP  I+ L+E IKDG  LI LLE ++G+KL  EQ + +++IH ++NI  AL+FL+ R+IKLVNIN+TDIADGRPSI+LGL+WT+                  +          +   A       K+      +AK +LL WV  T   +  + V DFGPSWR+G AF+++++ I P LVD +   +K +TN+ENL+EAF  AE +LGIP LLD +DVDV KPDEKSIMTYI QFLK YP+
Sbjct:    5 YSETLNQYVISFLQRHPPMVINELFEDIKDGTKLIALLEVLSGQKLPCEQGRQLKRIHWVANIGAALKFLEGRRIKLVNINSTDIADGRPSIVLGLIWTIILYFQIEELTSNLPQLHAMSSSTSSVDSVVSSEAASPPIKRKIVNKIQGSAKKALLRWVQYTAAKRLGIEVKDFGPSWRNGIAFHSVIYAIRPDLVDMDIEKLKRRTNRENLEEAFSIAENELGIPRLLDPEDVDVDKPDEKSIMTYIAQFLKHYPD 261

[Close]

BLAST of Nesprin-1 vs. Ensembl Xenopus
Match: SYNE1 (spectrin repeat containing, nuclear envelope 1 [Source:NCBI gene;Acc:100145637])

HSP 1 Score: 220.32 bits (560), Expect = 1.797e-63
Identity = 118/257 (45.91%), Postives = 162/257 (63.04%), Query Frame = 3

Query:  243 FTNWINTYLV---QAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTV------------------XXXXXXXXXXXXXXGALGADYDGKL------NAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWN--GVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            ++  +N Y++   Q HPP  I+ L+E IKDG  LI LLE ++G+KL  EQ + +++IH ++NI  AL+FL+ R+IKLVNIN+TDIADGRPSI+LGL+WT+                  +          +   A       K+      +AK +LL WV  T   +  + V DFGPSWR+G AF+++++ I P LVD +   +K +TN+ENL+EAF  AE +LGIP LLD +DVDV KPDEKSIMTYI QFLK YP+
Sbjct:    5 YSETLNQYVISFLQRHPPMVINELFEDIKDGTKLIALLEVLSGQKLPCEQGRQLKRIHWVANIGAALKFLEGRRIKLVNINSTDIADGRPSIVLGLIWTIILYFQIEELTSNLPQLHAMSSSTSSVDSVVSSEAASPPIKRKIVNKIQGSAKKALLRWVQYTAAKRLGIEVKDFGPSWRNGIAFHSVIYAIRPDLVDMDIEKLKRRTNRENLEEAFSIAENELGIPRLLDPEDVDVDKPDEKSIMTYIAQFLKHYPD 261

[Close]

BLAST of Nesprin-1 vs. Ensembl Xenopus
Match: SYNE1 (spectrin repeat containing, nuclear envelope 1 [Source:NCBI gene;Acc:100145637])

HSP 1 Score: 219.935 bits (559), Expect = 2.052e-63
Identity = 118/257 (45.91%), Postives = 162/257 (63.04%), Query Frame = 3

Query:  243 FTNWINTYLV---QAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTV------------------XXXXXXXXXXXXXXGALGADYDGKL------NAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWN--GVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            ++  +N Y++   Q HPP  I+ L+E IKDG  LI LLE ++G+KL  EQ + +++IH ++NI  AL+FL+ R+IKLVNIN+TDIADGRPSI+LGL+WT+                  +          +   A       K+      +AK +LL WV  T   +  + V DFGPSWR+G AF+++++ I P LVD +   +K +TN+ENL+EAF  AE +LGIP LLD +DVDV KPDEKSIMTYI QFLK YP+
Sbjct:    5 YSETLNQYVISFLQRHPPMVINELFEDIKDGTKLIALLEVLSGQKLPCEQGRQLKRIHWVANIGAALKFLEGRRIKLVNINSTDIADGRPSIVLGLIWTIILYFQIEELTSNLPQLHAMSSSTSSVDSVVSSEAASPPIKRKIVNKIQGSAKKALLRWVQYTAAKRLGIEVKDFGPSWRNGIAFHSVIYAIRPDLVDMDIEKLKRRTNRENLEEAFSIAENELGIPRLLDPEDVDVDKPDEKSIMTYIAQFLKHYPD 261

[Close]

BLAST of Nesprin-1 vs. Ensembl Xenopus
Match: SYNE1 (spectrin repeat containing, nuclear envelope 1 [Source:NCBI gene;Acc:100145637])

HSP 1 Score: 219.935 bits (559), Expect = 2.710e-63
Identity = 121/265 (45.66%), Postives = 165/265 (62.26%), Query Frame = 3

Query:  219 QERVQRKTFTNWINTYLV---QAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTV------------------XXXXXXXXXXXXXXGALGADYDGKL------NAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWN--GVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            Q R+ +   T  +N Y++   Q HPP  I+ L+E IKDG  LI LLE ++G+KL  EQ + +++IH ++NI  AL+FL+ R+IKLVNIN+TDIADGRPSI+LGL+WT+                  +          +   A       K+      +AK +LL WV  T   +  + V DFGPSWR+G AF+++++ I P LVD +   +K +TN+ENL+EAF  AE +LGIP LLD +DVDV KPDEKSIMTYI QFLK YP+
Sbjct:    3 QARIDQSIVT--LNQYVISFLQRHPPMVINELFEDIKDGTKLIALLEVLSGQKLPCEQGRQLKRIHWVANIGAALKFLEGRRIKLVNINSTDIADGRPSIVLGLIWTIILYFQIEELTSNLPQLHAMSSSTSSVDSVVSSEAASPPIKRKIVNKIQGSAKKALLRWVQYTAAKRLGIEVKDFGPSWRNGIAFHSVIYAIRPDLVDMDIEKLKRRTNRENLEEAFSIAENELGIPRLLDPEDVDVDKPDEKSIMTYIAQFLKHYPD 265

[Close]

BLAST of Nesprin-1 vs. Ensembl Mouse
Match: Syne1 (spectrin repeat containing, nuclear envelope 1 [Source:MGI Symbol;Acc:MGI:1927152])

HSP 1 Score: 251.521 bits (641), Expect = 6.865e-78
Identity = 130/265 (49.06%), Postives = 170/265 (64.15%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTV--------------------------XXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            +DEQE VQ++TFT WIN++L +  PP  +D+L+E +KDG+ L+ LLE ++G+KL  EQ   +++IH ++NI TAL+FL+ RKIKLVNINATDIADGRPSI+LGLMWT+                                E         + A   G  NAK +LL WV  T G +  + V DFG SWR G AF++++H I P LVD   VK ++N+ENL++AF  AE QLGIP LLD +DVDV KPDEKSIMTY+ QFL  YP+
Sbjct:   22 QDEQEIVQKRTFTKWINSHLAKRKPPMVVDDLFEDMKDGIKLLALLEVLSGQKLPCEQGHRVKRIHAVANIGTALKFLEGRKIKLVNINATDIADGRPSIVLGLMWTIILYFQIEELTSNLPQLQSLSSSASSVDSMVSTETASPPSKRKVAAKIQG--NAKKTLLKWVQHTAGKQMGIEVKDFGKSWRTGLAFHSVIHAIQPELVDLEKVKTRSNRENLEDAFTIAETQLGIPRLLDPEDVDVDKPDEKSIMTYVAQFLTQYPD 284

[Close]

BLAST of Nesprin-1 vs. Ensembl Mouse
Match: Syne1 (spectrin repeat containing, nuclear envelope 1 [Source:MGI Symbol;Acc:MGI:1927152])

HSP 1 Score: 246.514 bits (628), Expect = 9.807e-73
Identity = 130/272 (47.79%), Postives = 170/272 (62.50%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRK-------IKLVNINATDIADGRPSIILGLMWTV--------------------------XXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            +DEQE VQ++TFT WIN++L +  PP  +D+L+E +KDG+ L+ LLE ++G+KL  EQ   +++IH ++NI TAL+FL+ RK       IKLVNINATDIADGRPSI+LGLMWT+                                E         + A   G  NAK +LL WV  T G +  + V DFG SWR G AF++++H I P LVD   VK ++N+ENL++AF  AE QLGIP LLD +DVDV KPDEKSIMTY+ QFL  YP+
Sbjct:   22 QDEQEIVQKRTFTKWINSHLAKRKPPMVVDDLFEDMKDGIKLLALLEVLSGQKLPCEQGHRVKRIHAVANIGTALKFLEGRKSMYRGSPIKLVNINATDIADGRPSIVLGLMWTIILYFQIEELTSNLPQLQSLSSSASSVDSMVSTETASPPSKRKVAAKIQG--NAKKTLLKWVQHTAGKQMGIEVKDFGKSWRTGLAFHSVIHAIQPELVDLEKVKTRSNRENLEDAFTIAETQLGIPRLLDPEDVDVDKPDEKSIMTYVAQFLTQYPD 291

[Close]

BLAST of Nesprin-1 vs. Ensembl Mouse
Match: Syne1 (spectrin repeat containing, nuclear envelope 1 [Source:MGI Symbol;Acc:MGI:1927152])

HSP 1 Score: 232.646 bits (592), Expect = 7.310e-68
Identity = 130/272 (47.79%), Postives = 170/272 (62.50%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRK-------IKLVNINATDIADGRPSIILGLMWTV--------------------------XXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            +DEQE VQ++TFT WIN++L +  PP  +D+L+E +KDG+ L+ LLE ++G+KL  EQ   +++IH ++NI TAL+FL+ RK       IKLVNINATDIADGRPSI+LGLMWT+                                E         + A   G  NAK +LL WV  T G +  + V DFG SWR G AF++++H I P LVD   VK ++N+ENL++AF  AE QLGIP LLD +DVDV KPDEKSIMTY+ QFL  YP+
Sbjct:   22 QDEQEIVQKRTFTKWINSHLAKRKPPMVVDDLFEDMKDGIKLLALLEVLSGQKLPCEQGHRVKRIHAVANIGTALKFLEGRKSMYRGSPIKLVNINATDIADGRPSIVLGLMWTIILYFQIEELTSNLPQLQSLSSSASSVDSMVSTETASPPSKRKVAAKIQG--NAKKTLLKWVQHTAGKQMGIEVKDFGKSWRTGLAFHSVIHAIQPELVDLEKVKTRSNRENLEDAFTIAETQLGIPRLLDPEDVDVDKPDEKSIMTYVAQFLTQYPD 291

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BLAST of Nesprin-1 vs. Ensembl Mouse
Match: Macf1 (microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559])

HSP 1 Score: 204.527 bits (519), Expect = 6.799e-63
Identity = 115/262 (43.89%), Postives = 161/262 (61.45%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSY---PEGGKEIEENIVATNNARKKQLY 986
            +DE++RVQ+KTFT W+N +L++      I++LYE ++DG  LI LLE ++G KL +E  K   + HRL N+  AL+FLK R++KLVNI   DI DG P + LGL+WT+IL FQI +              G ++AK  LL W  K       +  T+F   W DGK FNAL+H   P LVD   V+ ++N+ENL++AF+ AE+LG+  LLD +DVDV  PDEKS++TY++    ++   PEGG    E I AT    + Q Y
Sbjct:   17 RDERDRVQKKTFTKWVNKHLMKVRK--HINDLYEDLRDGHNLISLLEVLSGIKLPRE--KGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDIYISGES-------GDMSAKEKLLLWTQKVTAGYTGVKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQVQSNRENLEQAFEVAERLGVTRLLDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGG----EGISATEVDSRWQEY 263

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BLAST of Nesprin-1 vs. Ensembl Mouse
Match: Macf1 (microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559])

HSP 1 Score: 209.92 bits (533), Expect = 5.437e-60
Identity = 119/277 (42.96%), Postives = 167/277 (60.29%), Query Frame = 3

Query:  165 HDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSY---PEGGKEIEENIVATNNARKKQLY 986
             DSV    E  ++   DE++RVQ+KTFT W+N +L++      I++LYE ++DG  LI LLE ++G KL +E  K   + HRL N+  AL+FLK R++KLVNI   DI DG P + LGL+WT+IL FQI +          +   G ++AK  LL W  K       +  T+F   W DGK FNAL+H   P LVD   V+ ++N+ENL++AF+ AE+LG+  LLD +DVDV  PDEKS++TY++    ++   PEGG    E I AT    + Q Y
Sbjct:   58 QDSVLDPAERAVVRVADERDRVQKKTFTKWVNKHLMKVRK--HINDLYEDLRDGHNLISLLEVLSGIKLPRE--KGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDIYI-------SGESGDMSAKEKLLLWTQKVTAGYTGVKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQVQSNRENLEQAFEVAERLGVTRLLDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGG----EGISATEVDSRWQEY 319

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BLAST of Nesprin-1 vs. UniProt/SwissProt
Match: sp|Q8NF91|SYNE1_HUMAN (Nesprin-1 OS=Homo sapiens OX=9606 GN=SYNE1 PE=1 SV=4)

HSP 1 Score: 252.677 bits (644), Expect = 6.012e-74
Identity = 135/265 (50.94%), Postives = 178/265 (67.17%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXX--------------------------XXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            +DEQE VQ++TFT WIN++L +  PP  +D+L+E +KDG+ L+ LLE ++G+KL  EQ + M++IH ++NI TAL+FL+ RKIKLVNIN+TDIADGRPSI+LGLMWT+IL+FQIEE                                  +     G  NAK +LL WV  T G +  + V DFG SWR G AF++++H I P LVD   VK ++N+ENL++AF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK YP+
Sbjct:   22 QDEQEIVQKRTFTKWINSHLAKRKPPMVVDDLFEDMKDGVKLLALLEVLSGQKLPCEQGRRMKRIHAVANIGTALKFLEGRKIKLVNINSTDIADGRPSIVLGLMWTIILYFQIEELTSNLPQLQSLSSSASSVDSIVSSETPSPPSKRKVTTKIQG--NAKKALLKWVQYTAGKQTGIEVKDFGKSWRSGVAFHSVIHAIRPELVDLETVKGRSNRENLEDAFTIAETELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHYPD 284

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BLAST of Nesprin-1 vs. UniProt/SwissProt
Match: sp|Q6ZWR6|SYNE1_MOUSE (Nesprin-1 OS=Mus musculus OX=10090 GN=Syne1 PE=1 SV=2)

HSP 1 Score: 239.58 bits (610), Expect = 2.019e-69
Identity = 130/265 (49.06%), Postives = 170/265 (64.15%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTV--------------------------XXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            +DEQE VQ++TFT WIN++L +  PP  +D+L+E +KDG+ L+ LLE ++G+KL  EQ   +++IH ++NI TAL+FL+ RKIKLVNINATDIADGRPSI+LGLMWT+                                E         + A   G  NAK +LL WV  T G +  + V DFG SWR G AF++++H I P LVD   VK ++N+ENL++AF  AE QLGIP LLD +DVDV KPDEKSIMTY+ QFL  YP+
Sbjct:   22 QDEQEIVQKRTFTKWINSHLAKRKPPMVVDDLFEDMKDGIKLLALLEVLSGQKLPCEQGHRVKRIHAVANIGTALKFLEGRKIKLVNINATDIADGRPSIVLGLMWTIILYFQIEELTSNLPQLQSLSSSASSVDSMVSTETASPPSKRKVAAKIQG--NAKKTLLKWVQHTAGKQMGIEVKDFGKSWRTGLAFHSVIHAIQPELVDLEKVKTRSNRENLEDAFTIAETQLGIPRLLDPEDVDVDKPDEKSIMTYVAQFLTQYPD 284

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BLAST of Nesprin-1 vs. UniProt/SwissProt
Match: sp|Q9UPN3|MACF1_HUMAN (Microtubule-actin cross-linking factor 1, isoforms 1/2/3/5 OS=Homo sapiens OX=9606 GN=MACF1 PE=1 SV=4)

HSP 1 Score: 209.92 bits (533), Expect = 4.107e-59
Identity = 119/277 (42.96%), Postives = 167/277 (60.29%), Query Frame = 3

Query:  165 HDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSY---PEGGKEIEENIVATNNARKKQLY 986
             DSV    E  ++   DE++RVQ+KTFT W+N +L++      I++LYE ++DG  LI LLE ++G KL +E  K   + HRL N+  AL+FLK R++KLVNI   DI DG P + LGL+WT+IL FQI +          +   G ++AK  LL W  K       +  T+F   W DGK FNAL+H   P LVD   V+ ++N+ENL++AF+ AE+LG+  LLD +DVDV  PDEKS++TY++    ++   PEGG    E I AT    + Q Y
Sbjct:   58 QDSVLDPAERAVVRVADERDRVQKKTFTKWVNKHLMKVRK--HINDLYEDLRDGHNLISLLEVLSGIKLPRE--KGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDIYI-------SGESGDMSAKEKLLLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQIQSNRENLEQAFEVAERLGVTRLLDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGG----EGISATEVDSRWQEY 319

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BLAST of Nesprin-1 vs. UniProt/SwissProt
Match: sp|D3ZHV2|MACF1_RAT (Microtubule-actin cross-linking factor 1 OS=Rattus norvegicus OX=10116 GN=Macf1 PE=1 SV=1)

HSP 1 Score: 209.92 bits (533), Expect = 4.129e-59
Identity = 119/277 (42.96%), Postives = 167/277 (60.29%), Query Frame = 3

Query:  165 HDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSY---PEGGKEIEENIVATNNARKKQLY 986
             DSV    E  ++   DE++RVQ+KTFT W+N +L++      I++LYE ++DG  LI LLE ++G KL +E  K   + HRL N+  AL+FLK R++KLVNI   DI DG P + LGL+WT+IL FQI +          +   G ++AK  LL W  K       +  T+F   W DGK FNAL+H   P LVD   V+ ++N+ENL++AF+ AE+LG+  LLD +DVDV  PDEKS++TY++    ++   PEGG    E I AT    + Q Y
Sbjct:   58 QDSVLDPAERAVVRVADERDRVQKKTFTKWVNKHLMKVRK--HINDLYEDLRDGHNLISLLEVLSGIKLPRE--KGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDIYI-------SGESGDMSAKEKLLLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQIQSNRENLEQAFEVAERLGVTRLLDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGG----EGISATEVDSRWQEY 319

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BLAST of Nesprin-1 vs. UniProt/SwissProt
Match: sp|Q9QXZ0|MACF1_MOUSE (Microtubule-actin cross-linking factor 1 OS=Mus musculus OX=10090 GN=Macf1 PE=1 SV=2)

HSP 1 Score: 209.92 bits (533), Expect = 4.146e-59
Identity = 119/277 (42.96%), Postives = 167/277 (60.29%), Query Frame = 3

Query:  165 HDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSY---PEGGKEIEENIVATNNARKKQLY 986
             DSV    E  ++   DE++RVQ+KTFT W+N +L++      I++LYE ++DG  LI LLE ++G KL +E  K   + HRL N+  AL+FLK R++KLVNI   DI DG P + LGL+WT+IL FQI +          +   G ++AK  LL W  K       +  T+F   W DGK FNAL+H   P LVD   V+ ++N+ENL++AF+ AE+LG+  LLD +DVDV  PDEKS++TY++    ++   PEGG    E I AT    + Q Y
Sbjct:   58 QDSVLDPAERAVVRVADERDRVQKKTFTKWVNKHLMKVRK--HINDLYEDLRDGHNLISLLEVLSGIKLPRE--KGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDIYI-------SGESGDMSAKEKLLLWTQKVTAGYTGVKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQVQSNRENLEQAFEVAERLGVTRLLDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGG----EGISATEVDSRWQEY 319

[Close]

BLAST of Nesprin-1 vs. TrEMBL
Match: A0A0X3P4L6 (Uncharacterized protein (Fragment) OS=Schistocephalus solidus OX=70667 GN=TR127326 PE=4 SV=1)

HSP 1 Score: 296.204 bits (757), Expect = 7.974e-87
Identity = 150/253 (59.29%), Postives = 195/253 (77.08%), Query Frame = 3

Query:  186 TETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDG---KLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQA-EQLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGK 932
             E  I S++DEQERVQ+KTFTNW+NTYL + +PP ++ NLYE IKDG+CLI LLE ++ E L  +   +M++ H LSN+ TALEFLKSR++KLVNINA+DI DG+P+I+LGL+W++IL+FQIEEQE++L+  LG    G   K  AK +L  W+     +KYNL++ DFGPSWRDG AFNA+VH+I+P LVD + V N++N+ENL  AF  A E+LGIP LLD +DVDV KPDEKSIMTY+ QF K+YPE GK
Sbjct:  211 AEQKISSRRDEQERVQKKTFTNWVNTYLSKVNPPIRVGNLYEDIKDGICLIRLLEVLSRESLPIDVAASMKRAHCLSNVRTALEFLKSRQLKLVNINASDIVDGKPTIVLGLIWSIILYFQIEEQEDMLLELLGISRGGVHSKTTAKKALTTWIQNAFSEKYNLDIKDFGPSWRDGVAFNAIVHSINPDLVDMHEVANRSNRENLHRAFSLAEEELGIPKLLDPEDVDVDKPDEKSIMTYVAQFYKAYPEAGK 463

[Close]

BLAST of Nesprin-1 vs. TrEMBL
Match: A0A1I8GMD0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 286.574 bits (732), Expect = 2.819e-85
Identity = 155/275 (56.36%), Postives = 207/275 (75.27%), Query Frame = 3

Query:  192 TLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGAL--------GADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQ-LGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGK---EIEENIVATNNARKKQ 980
            T + S +DEQERVQ+KTFTNWINTYL+   PP KI +L+  IKDG  L+ LLE ++G KL+KE   NMQ++H LSN+ TAL FL+S++IKLVNIN  DI DG+P+I+LGLMWT+IL+FQIEEQE+++  +L        G  + G  +AK +LL W    +GDKY++++ DFG SWRDG AFNA+VHNIDPSLVD + +++++N+ENL+ AF+ AE  L IP LLD +DVDV KPDEKSIMTYI QFLK+YPEGGK   ++++ + A   A +K+
Sbjct:    8 TNVKSLRDEQERVQKKTFTNWINTYLITCQPPCKISDLFTEIKDGTRLLLLLEVLSGNKLQKENRGNMQRVHCLSNVRTALSFLESKQIKLVNINPADIVDGKPTIVLGLMWTIILYFQIEEQEDMIRKSLEGTELAERGELFKG--SAKKALLAWAQNNLGDKYDVDLKDFGSSWRDGAAFNAMVHNIDPSLVDMDALRSRSNRENLEAAFQAAENGLNIPRLLDAEDVDVDKPDEKSIMTYIAQFLKAYPEGGKNRPKLQDQLDAARQAGEKE 280

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BLAST of Nesprin-1 vs. TrEMBL
Match: A0A0X3P9Y2 (Nesprin-1 (Fragment) OS=Schistocephalus solidus OX=70667 GN=SYNE1 PE=4 SV=1)

HSP 1 Score: 290.041 bits (741), Expect = 1.369e-84
Identity = 150/259 (57.92%), Postives = 195/259 (75.29%), Query Frame = 3

Query:  186 TETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDG---KLNAKTSLLNWVNKTIGD------KYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQA-EQLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGK 932
             E  I S++DEQERVQ+KTFTNW+NTYL + +PP ++ NLYE IKDG+CLI LLE ++ E L  +   +M++ H LSN+ TALEFLKSR++KLVNINA+DI DG+P+I+LGL+W++IL+FQIEEQE++L+  LG    G   K  AK +L  W+     +      KYNL++ DFGPSWRDG AFNA+VH+I+P LVD + V N++N+ENL  AF  A E+LGIP LLD +DVDV KPDEKSIMTY+ QF K+YPE GK
Sbjct:  211 AEQKISSRRDEQERVQKKTFTNWVNTYLSKVNPPIRVGNLYEDIKDGICLIRLLEVLSRESLPIDVAASMKRAHCLSNVRTALEFLKSRQLKLVNINASDIVDGKPTIVLGLIWSIILYFQIEEQEDMLLELLGISRGGVHSKTTAKKALTTWIQNAFSERISRHRKYNLDIKDFGPSWRDGVAFNAIVHSINPDLVDMHEVANRSNRENLHRAFSLAEEELGIPKLLDPEDVDVDKPDEKSIMTYVAQFYKAYPEAGK 469

[Close]

BLAST of Nesprin-1 vs. TrEMBL
Match: A0A5K3ELW0 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 261.922 bits (668), Expect = 5.326e-82
Identity = 145/258 (56.20%), Postives = 186/258 (72.09%), Query Frame = 3

Query:  189 ETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYD---GKLNAKTSLLNWVNKTIGDK------YNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQ-LGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGK 932
            E  I SK+ EQE VQ+KTFTNW+NTYL + +PP +++NL+E IKDG+ LI LLE ++ E L       M+  HRLSN+ TAL+FL +RKIKLVN+N  DI DGRP I+LGL+WT+IL+FQIEEQEE+L+  LG        K+ AK +L  WV      +      +NL++ DFGPSWRDG AFNA+VH+IDP+LVD   ++ K+N+ENL  AF  AE+ LGIP +LD +DVDV KPDEKSIMTY+ QF K+YPE GK
Sbjct:   40 EQKISSKRGEQEHVQKKTFTNWMNTYLSKINPPLQVNNLFEDIKDGIILIRLLEVLSKESLPVNIATAMKPAHRLSNVKTALDFLNTRKIKLVNVNPLDIVDGRPKIVLGLIWTIILYFQIEEQEEMLLELLGLSKSTGQSKITAKQALTTWVQNAFSQRITRYRQFNLHINDFGPSWRDGVAFNAIVHSIDPNLVDMKDLERKSNRENLSMAFTVAEEKLGIPKILDPEDVDVDKPDEKSIMTYVAQFYKAYPETGK 297

[Close]

BLAST of Nesprin-1 vs. TrEMBL
Match: A0A2R2MSB1 (nesprin-1-like OS=Lingula unguis OX=7574 GN=LOC106156515 PE=4 SV=1)

HSP 1 Score: 282.337 bits (721), Expect = 7.308e-82
Identity = 138/253 (54.55%), Postives = 182/253 (71.94%), Query Frame = 3

Query:  213 DEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXX--XXXXXXXXXXXXGALGADYDG-------------KLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQ-LGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            DEQERVQ+KTFTNWIN+YL +  PP K+ +L+E  KDG CL++LLE ++GEK+  E+ K+ +++H LSN+ TAL++L+SRKIKLVNIN TDI DG+PSI+LGLMWT+IL    +   +    + A  AD DG             K+ AK +LL W   T+  K  + + DFGPSWRDG+AF+A+VH+I P L+D N + NK+NKENL+ AF  AE+ LGIP LLD +DVDV+KPDEKSIMTY+ QFL  YP+
Sbjct:   89 DEQERVQKKTFTNWINSYLCKRRPPIKVKDLFEDFKDGTCLLYLLEVLSGEKMAIEKGKHCKRLHFLSNVTTALKYLESRKIKLVNINPTDIVDGKPSIVLGLMWTIILYFQIEENAKLLEQLEASLADMDGEPPEKKMSAKDRFKMGAKKALLVWTKHTVAKKVEVEIKDFGPSWRDGRAFSAMVHSIRPELIDMNLLPNKSNKENLETAFNAAEEHLGIPRLLDPEDVDVSKPDEKSIMTYVAQFLSKYPD 341

[Close]

BLAST of Nesprin-1 vs. Ensembl Cavefish
Match: ENSAMXT00000036923.1 (pep primary_assembly:Astyanax_mexicanus-2.0:9:24166584:24185412:1 gene:ENSAMXG00000043596.1 transcript:ENSAMXT00000036923.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 240.35 bits (612), Expect = 3.376e-75
Identity = 128/271 (47.23%), Postives = 173/271 (63.84%), Query Frame = 3

Query:  213 DEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRK-------IKLVNINATDIADGRPSIILGLMWT--------------------------VXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            +EQE VQ++TFT WIN++L + +PP +I++L+E IKDG+ L+ LLE ++G++L  EQ + +++IH +SNI TAL+FL+ R+       IKLVNINATDIADGRPSI+LGLMWT                          V      E     +   +   + G  NAK +LL WV  T   +  + V DFG SWR G AF+++++ I P LVD   VK ++N+ENL+EAF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK YP+
Sbjct:   12 NEQESVQKRTFTKWINSHLAKRNPPLEINDLFEDIKDGVKLLALLEVLSGQRLPSEQGRQLKRIHWVSNIGTALKFLEGRRSVYRGSPIKLVNINATDIADGRPSIVLGLMWTIILYFQIEELTSNLPALQALSNSASSVDSMASSETASPPMKRKVVTKFQG--NAKRALLRWVQCTATKRLGIEVKDFGSSWRTGVAFHSVIYAIRPDLVDMELVKRRSNRENLEEAFTVAENELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHYPD 280

[Close]

BLAST of Nesprin-1 vs. Ensembl Cavefish
Match: ENSAMXT00000039764.1 (pep primary_assembly:Astyanax_mexicanus-2.0:9:24167966:24185412:1 gene:ENSAMXG00000043596.1 transcript:ENSAMXT00000039764.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 240.35 bits (612), Expect = 3.872e-75
Identity = 128/270 (47.41%), Postives = 172/270 (63.70%), Query Frame = 3

Query:  216 EQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRK-------IKLVNINATDIADGRPSIILGLMWT--------------------------VXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            EQE VQ++TFT WIN++L + +PP +I++L+E IKDG+ L+ LLE ++G++L  EQ + +++IH +SNI TAL+FL+ R+       IKLVNINATDIADGRPSI+LGLMWT                          V      E     +   +   + G  NAK +LL WV  T   +  + V DFG SWR G AF+++++ I P LVD   VK ++N+ENL+EAF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK YP+
Sbjct:   10 EQESVQKRTFTKWINSHLAKRNPPLEINDLFEDIKDGVKLLALLEVLSGQRLPSEQGRQLKRIHWVSNIGTALKFLEGRRSVYRGSPIKLVNINATDIADGRPSIVLGLMWTIILYFQIEELTSNLPALQALSNSASSVDSMASSETASPPMKRKVVTKFQG--NAKRALLRWVQCTATKRLGIEVKDFGSSWRTGVAFHSVIYAIRPDLVDMELVKRRSNRENLEEAFTVAENELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHYPD 277

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BLAST of Nesprin-1 vs. Ensembl Cavefish
Match: ENSAMXT00000045503.1 (pep primary_assembly:Astyanax_mexicanus-2.0:9:24166584:24193162:1 gene:ENSAMXG00000043596.1 transcript:ENSAMXT00000045503.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 243.047 bits (619), Expect = 1.203e-71
Identity = 128/271 (47.23%), Postives = 173/271 (63.84%), Query Frame = 3

Query:  213 DEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRK-------IKLVNINATDIADGRPSIILGLMWT--------------------------VXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            +EQE VQ++TFT WIN++L + +PP +I++L+E IKDG+ L+ LLE ++G++L  EQ + +++IH +SNI TAL+FL+ R+       IKLVNINATDIADGRPSI+LGLMWT                          V      E     +   +   + G  NAK +LL WV  T   +  + V DFG SWR G AF+++++ I P LVD   VK ++N+ENL+EAF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK YP+
Sbjct:   12 NEQESVQKRTFTKWINSHLAKRNPPLEINDLFEDIKDGVKLLALLEVLSGQRLPSEQGRQLKRIHWVSNIGTALKFLEGRRSVYRGSPIKLVNINATDIADGRPSIVLGLMWTIILYFQIEELTSNLPALQALSNSASSVDSMASSETASPPMKRKVVTKFQG--NAKRALLRWVQCTATKRLGIEVKDFGSSWRTGVAFHSVIYAIRPDLVDMELVKRRSNRENLEEAFTVAENELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHYPD 280

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BLAST of Nesprin-1 vs. Ensembl Cavefish
Match: syne1a (spectrin repeat containing, nuclear envelope 1a [Source:ZFIN;Acc:ZDB-GENE-030131-4172])

HSP 1 Score: 236.113 bits (601), Expect = 3.307e-69
Identity = 132/268 (49.25%), Postives = 176/268 (65.67%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGA--------------------------DYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGK 932
            +DEQE VQ++TFT WIN++L +  PP  + +LYE IKDG+ L+ LLE ++G KL  EQ + +++IH L+N+ TAL FL+ R+IKLVNIN TD+ DGRPSI+LGL+WT+IL+FQIEE    L    G                            + G   AK +LL WV +T   +  L+V DFGPSWR G AF+A++  + P LV+   V N+ N+ NL+EAF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK +P+GG+
Sbjct:   36 RDEQEAVQKRTFTKWINSHLAKRDPPLVVKDLYEDIKDGVMLLALLEVLSGHKLPCEQGRKLRRIHWLANVGTALRFLEGRRIKLVNINTTDVVDGRPSIVLGLIWTIILYFQIEELTSSLPPQKGQNSSNSSVDSSNSTETASPPVKRKPRLSFQG--GAKRALLKWVQRTATKRLGLDVKDFGPSWRSGVAFHAVIFALRPQLVNMERVWNRPNRANLEEAFSLAERELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHHPDGGE 301

[Close]

BLAST of Nesprin-1 vs. Ensembl Cavefish
Match: MACF1 (microtubule actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664])

HSP 1 Score: 202.601 bits (514), Expect = 2.353e-59
Identity = 112/261 (42.91%), Postives = 159/261 (60.92%), Query Frame = 3

Query:  165 HDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSY---PEGGKEI 938
             DSV    E  ++   DE++RVQ+KTFT W+N +L++      I +LYE ++DG  LI LLE ++G +L +E  K   + HRL N+  AL+FLK R++KLVNI   DI DG P + LGL+WT+IL FQI E          +   G L AK  LL W  +       L  ++F  SW +G+ FNAL+H   P L+D   V  ++N+ENL++AF+ AE LG+  LLD +DVDV  PDEKS++TY++    ++   PEGG+ I
Sbjct:   49 QDSVLDPAERAVVRVADERDRVQKKTFTKWVNKHLIKVRK--HITDLYEDLRDGHNLISLLEVLSGVQLPRE--KGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISEIYV-------SGESGDLTAKEKLLLWTQQATEGYPGLRCSNFSSSWSNGRMFNALLHRYRPDLIDMEVVARQSNRENLEQAFEIAESLGVTRLLDAEDVDVPSPDEKSVITYVSSIYDAFPKIPEGGEGI 298

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BLAST of Nesprin-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009426.1 (pep scaffold:Pmarinus_7.0:GL476614:383620:553344:1 gene:ENSPMAG00000008375.1 transcript:ENSPMAT00000009426.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 218.394 bits (555), Expect = 1.087e-63
Identity = 127/275 (46.18%), Postives = 172/275 (62.55%), Query Frame = 3

Query:  213 DEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXX---------------XXXXXXXXXGALGADYDGKL-------NAKTSLLNWVNKTIGDKYN----LNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQ-LGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGKEIEENIVA 956
            DEQE VQ++TFT WIN++L    PP  +D+L++ ++DG+ LI LLE +TGE+L  EQ + +++IH LSNI TAL+F+++R+IKLVNINA  IADG+PSI+LGL+WT+IL+FQ                E     + G+  A    K         AK +LL WV   +    +      + DFG SWR G AF+ ++  I P LV+   V  ++N ENL+EAF  AE+ LGIP LLD +DVDV KPDEK IMTY+ QFLK YP    E E N++ 
Sbjct:    1 DEQEAVQKRTFTKWINSHLALVRPPLVVDDLFKDLRDGVKLIALLEVLTGERLPYEQGRQLKRIHALSNIGTALKFMEARRIKLVNINAPYIADGKPSIVLGLIWTIILYFQIEEATNNLLPISPWSSETSLSSMEGSSTASPQRKRRQTPMRSTAKKALLKWVQLVVPSLESRVGGTEIRDFGASWRTGIAFHLMIRAIQPELVNLEQVTQRSNHENLEEAFIVAEEKLGIPRLLDPKDVDVDKPDEKIIMTYVAQFLKHYPNAA-ESEGNLLG 274

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BLAST of Nesprin-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002838.1 (pep scaffold:Pmarinus_7.0:GL477168:22072:87306:1 gene:ENSPMAG00000002584.1 transcript:ENSPMAT00000002838.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 189.119 bits (479), Expect = 2.507e-54
Identity = 104/237 (43.88%), Postives = 151/237 (63.71%), Query Frame = 3

Query:  213 DEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQ-LGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYP 920
            DE++RVQ+KTFT W+N +L++      ++++YE ++DG  LI LLE ++GEKL +E  K   + HRL N+  AL+FL+ R+IKLVNI + DIADG P + LGL+WT+IL FQI + +       G   D  + AK  LL W  + +     +   +F  SWRDG+ FNA++H   P L+D   V  + N+ENL++AF  AE+ +G+  LLD +DVDV  PDEKSI+TY++      P
Sbjct:    6 DERDRVQKKTFTKWVNKHLMKVRK--HVNDMYEDLRDGHNLISLLEVLSGEKLPRE--KGRMRFHRLHNVQIALDFLRLRQIKLVNIRSDDIADGNPKLTLGLIWTIILHFQIADIQVS-----GQSED--MTAKDRLLLWSQRMVEGYPGIRCDNFTTSWRDGRLFNAIIHRHRPELIDMKRVAVRANRENLEQAFTVAERDMGVTRLLDPEDVDVPSPDEKSIITYVSSLYDVIP 231

[Close]

BLAST of Nesprin-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006515.1 (pep scaffold:Pmarinus_7.0:GL476558:286719:335794:-1 gene:ENSPMAG00000005865.1 transcript:ENSPMAT00000006515.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 176.022 bits (445), Expect = 4.090e-50
Identity = 94/230 (40.87%), Postives = 138/230 (60.00%), Query Frame = 3

Query:  213 DEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYIT 899
            DE++RVQ+KTFT WIN++L Q      I +LYE ++DG  LI LLE+++G+ + +E  K   ++H + NI   L +L+ +++KLVNI   DI DG P + LGL+WT+          ++  G +G    G    +  LL W      D   L+  +F  SWRDG+   A+VH   P LVD   V+ +  ++NL+EA   AE +LG+P LLD +DVDV  PDEKSI+TY++
Sbjct:    1 DERDRVQKKTFTKWINSHLQQVR--MNIGDLYEDLRDGQALISLLESLSGDCIIRE--KGRLRVHHVQNIQIVLNYLQHQQVKLVNIRKEDIVDGNPKLTLGLIWTI------ILHYQVSAGQVGGGTPG----RERLLAWARLVAKDVPGLSCHNFSSSWRDGRLLCAIVHAHRPGLVDLEEVRRRGARQNLEEALSLAESELGVPRLLDPEDVDVDAPDEKSIITYVS 216

[Close]

BLAST of Nesprin-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008111.1 (pep scaffold:Pmarinus_7.0:GL478136:8967:48859:1 gene:ENSPMAG00000007298.1 transcript:ENSPMAT00000008111.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 176.022 bits (445), Expect = 3.368e-49
Identity = 102/232 (43.97%), Postives = 145/232 (62.50%), Query Frame = 3

Query:  213 DEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQ-LGIPHLLDVQDVDVAKPDEKSIMTYITQF 905
            DE+E VQ+KTFT W+N++L  A    +I +LY  ++DG  LI LLE ++GEKL K     M +IH L N++ AL+FLK +K+ L N+ + DI DG   +ILGL+WT+IL FQI++           D   + +AK +LL W         N+N+ +F  SWRDG AFNA+VH   P L++++ +K      NLQ AF  AEQ LG+  LLD +D+ V +PDEKSI+TY+  +
Sbjct:   53 DEREAVQKKTFTKWVNSHL--ARVSCRITDLYTDLRDGRMLIKLLEVLSGEKLPKPTRGKM-RIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLILGLIWTIILRFQIQDISVETE-----DNKERRSAKDALLLWCQMKTAGYQNVNIHNFTTSWRDGLAFNAIVHKHRPDLIEFDKLKKSNAHHNLQNAFNIAEQKLGLTKLLDPEDISVDQPDEKSIITYVVTY 276

[Close]

BLAST of Nesprin-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006506.1 (pep scaffold:Pmarinus_7.0:GL476558:286440:335800:-1 gene:ENSPMAG00000005865.1 transcript:ENSPMAT00000006506.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 174.481 bits (441), Expect = 4.202e-49
Identity = 94/233 (40.34%), Postives = 138/233 (59.23%), Query Frame = 3

Query:  213 DEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDG---KLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYIT 899
            DE++RVQ+KTFT WIN++L Q      I +LYE ++DG  LI LLE+++G+ + +E  K   ++H + NI   L +L+ +++KLVNI   DI DG P + LGL+WT+               A  A  +G    + ++  LL W      D   L+  +F  SWRDG+   A+VH   P LVD   V+ +  ++NL+EA   AE +LG+P LLD +DVDV  PDEKSI+TY++
Sbjct:    3 DERDRVQKKTFTKWINSHLQQVR--MNIGDLYEDLRDGQALISLLESLSGDCIIRE--KGRLRVHHVQNIQIVLNYLQHQQVKLVNIRKEDIVDGNPKLTLGLIWTI---------ILHYQNASFAHIEGNHSSMQSRERLLAWARLVAKDVPGLSCHNFSSSWRDGRLLCAIVHAHRPGLVDLEEVRRRGARQNLEEALSLAESELGVPRLLDPEDVDVDAPDEKSIITYVS 222

[Close]

BLAST of Nesprin-1 vs. Ensembl Yeast
Match: SAC6 (Fimbrin, actin-bundling protein; cooperates with Scp1p in organization and maintenance of the actin cytoskeleton; phosphorylated by Cdc28p/Clb2p in metaphase on T103, to regulate conformation, and modulate actin filament binding affinity and actin cable dynamics; relocalizes from the plasma membrane to the cytoplasm upon DNA replication stress; human homologs PLS3 and LCP1 implicated in spinocerebellar ataxia type 2 (SCA2) can each complement yeast null mutant [Source:SGD;Acc:S000002536])

HSP 1 Score: 55.4546 bits (132), Expect = 6.317e-9
Identity = 55/252 (21.83%), Postives = 105/252 (41.67%), Query Frame = 3

Query:  231 QRKTFTNWINTYLVQAHP-----PYKIDN--LYESIKDGLCLIHLLEAMTGEKLRK------EQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDG--------KLNAKTSLLNWVNKTIGDK-YNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYP 920
            +R+ FT  IN+ L          P+  D   L++  +DGL L  L+     + +        ++ K +       N N  +   K+    +VN+++ DI +GR  +ILGL+W +I    + + +  L   L    +         +L  +  LL W N  +    +N  VT+F     DG+ +  L++ +DP+L     ++     E  ++  + AE+L     L    +    P  K  + ++     ++P
Sbjct:  141 ERREFTKHINSVLAGDQDIGDLLPFPTDTFQLFDECRDGLVLSKLINDSVPDTIDTRVLNWPKKGKELNNFQASENANIVINSAKAIGCVVVNVHSEDIIEGREHLILGLIWQIIRRGLLSKIDIKLHPELYRLLEDDETLEQFLRLPPEQILLRWFNYHLKQANWNRRVTNFSKDVSDGENYTILLNQLDPALCSKAPLQTTDLMERAEQVLQNAEKLDCRKYLTPSSLVAGNP--KLNLAFVAHLFNTHP 390

HSP 2 Score: 53.5286 bits (127), Expect = 2.164e-8
Identity = 63/247 (25.51%), Postives = 113/247 (45.75%), Query Frame = 3

Query:  216 EQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLE-AMTGE---KLRKEQPKNMQKIHR---LSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNA-----LVHNIDPSLVDWNGVKNKTNKE----NLQEAFKQAEQLGIPHLLDVQDVDVAKPDEKSIMTYITQFL 908
            E ER + + FT W+N+  +   PP  + +L++ +KDGL L+   E  M G    K   ++P +  +I R   L N N A++  +++   LV I  +DI DG   + LGL+W ++        + +          G+  + + +L W    +  K   N T    S++D    NA     +++ I P  VD++ V     +E    N + A   A +LG    L  +D++  +   + I+T+I   +
Sbjct:  409 EGER-EARVFTLWLNS--LDVDPP--VISLFDDLKDGLILLQAYEKVMPGAVDFKHVNKRPASGAEISRFKALENTNYAVDLGRAKGFSLVGIEGSDIVDGNKLLTLGLVWQLMRRNISITMKTLSSS-------GRDMSDSQILKWAQDQV-TKGGKNSTI--RSFKDQALSNAHFLLDVLNGIAPGYVDYDLVTPGNTEEERYANARLAISIARKLGALIWLVPEDINEVRA--RLIITFIASLM 638

[Close]

BLAST of Nesprin-1 vs. Ensembl Nematostella
Match: EDO42431 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1C0])

HSP 1 Score: 172.94 bits (437), Expect = 7.161e-48
Identity = 105/257 (40.86%), Postives = 158/257 (61.48%), Query Frame = 3

Query:  156 MEDHDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWV-NKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYP 920
            ME   + +    + I    DE+ERVQ+KTFT WIN++L +     +++ LY  ++DG  LI LLE ++GEKL K     M +IH L N+  +L FLK +++ L N+ A DI DG   I LGL+WT+IL FQI++        +  +   K +AK +LL W  +KT+G   ++ +T+F  SWRDG AFNA++H   P L+++  +     ++NL++AF+ AE QLGI  LLD +DV+V  PDEKSI+TY+  +   + 
Sbjct:    1 MEQQSAARLFERSRIKVLADERERVQKKTFTKWINSHLQRVGA--RVNELYHDLQDGRKLILLLEVLSGEKLPKPSKGRM-RIHNLENVEKSLVFLKKQRVHLENVGAHDIVDGNQKITLGLIWTIILRFQIQDI------TIEGETTEKRSAKDALLLWCQSKTVG-YVHVTITNFTTSWRDGLAFNAIIHRHRPDLIEFEKLTKADAEQNLEQAFEVAETQLGITPLLDAEDVNVDFPDEKSILTYVAAYYHYFA 247

[Close]

BLAST of Nesprin-1 vs. Ensembl Nematostella
Match: EDO29101 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T4L2])

HSP 1 Score: 159.458 bits (402), Expect = 1.064e-45
Identity = 101/278 (36.33%), Postives = 160/278 (57.55%), Query Frame = 3

Query:  138 YFEFSTMEDHDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQ-LGIPHLLDVQDVDVAKPDEKSIMTYITQF------LKSYPEGGKEIEENI 950
            Y E  T E     K + +TL    K+E+   Q+KTFT W+N++L +      +++L+E + DG  LI LLE ++GEK+     +   ++H + N+N ALEFL+ + +KL +I A D+ DG   +ILGL+W +IL FQI +       +       + +AK +LL W  +      ++++ +F  SWR+G AFNAL+H   P L+D+  ++   ++ NL  AF  AE+ LGI  LLD +DVD  +PD++S+MTY+  +      LKS   GG+ I + I
Sbjct:    3 YVEAPTFE-----KDRIKTL----KNERIFSQKKTFTKWVNSFLDKVRSGLHVNDLFEDLADGKILISLLEIISGEKI-GFAARGKFRVHNIQNVNKALEFLQ-KSVKLESIGAEDVVDGNERLILGLIWMIILRFQIADISYQDDMS-----KERKSAKEALLLWCQRMTRGYPDVDIQNFSTSWRNGLAFNALLHKHRPDLIDYATLRPSQHEANLNNAFTVAEEKLGITRLLDAEDVDCPRPDDRSVMTYVAAYYQYFAKLKSEETGGRRIAKII 264

[Close]

BLAST of Nesprin-1 vs. Ensembl Nematostella
Match: EDO37708 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SF38])

HSP 1 Score: 162.155 bits (409), Expect = 4.062e-44
Identity = 104/238 (43.70%), Postives = 154/238 (64.71%), Query Frame = 3

Query:  213 DEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAK-PDEKSIMTYITQFLKSYP 920
            D ++ VQ+KTFT W+N  L++     +I +LYE ++DG  L+ LLE +   KL +E  + + + H L N+ TALEFL+S+KIKLVNI + DI DG P + LGL+WT+IL+FQI + +  + G    D    L+AK +LL+W  K       ++V +F  SWRDG AFNA++H   P LVD++ +   + + N++ AF  AE +L +P LLDV+DV V++ PDEKSIMTY+    + +P
Sbjct:   33 DHRDAVQKKTFTKWVNNRLLKVG--VRILDLYEELRDGEDLLLLLEVLCNVKLPRE--RGLLRFHMLQNVQTALEFLESKKIKLVNIRSDDIVDGNPKLTLGLIWTIILYFQINDLDVEIAGEKVQD----LSAKDALLHWSKKVTQGYPKVDVKNFSSSWRDGFAFNAIIHRYRPDLVDFSKLSKTSPEANIEYAFHVAEKELNVPRLLDVEDVVVSENPDEKSIMTYVASLYEVFP 262

[Close]

BLAST of Nesprin-1 vs. Ensembl Nematostella
Match: EDO30309 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T157])

HSP 1 Score: 149.443 bits (376), Expect = 2.144e-43
Identity = 89/229 (38.86%), Postives = 137/229 (59.83%), Query Frame = 3

Query:  216 EQERVQRKTFTNWINTYLVQAHP--PYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQ-LGIPHLLDVQDVDVAKPDEKSIMTY 893
            E+E   RK FT W+N+ L   HP  P  I ++   ++DG  L++LLE +T +KL+KE  K   ++H+++N+  AL  L   KIKLV IN  DI DG+  +ILGL+W++IL FQ+EE  +  + A  A        +  LL W  + + D Y++ + DF  SW++G AF+A++H+ +P   D+  VK     E L+ AF+ AE    +P +LD  DV+V +PD+K I+ Y
Sbjct:    7 ERENAHRKAFTKWVNSKLAD-HPTEPCTIKDVVTDLQDGRMLLNLLEVLTRKKLKKE--KGNLRVHKINNVERALVLLDECKIKLVGINGFDIVDGKHRVILGLLWSIILRFQVEEVMQQEVNAESAK---SFTIEKRLLEWCRERL-DGYDVEINDFSSSWQNGLAFSAILHSYNPEAFDYTEVKQMGTTERLEHAFQSAEDGFDVPRILDASDVNVPRPDKKLIILY 228

[Close]

BLAST of Nesprin-1 vs. Ensembl Nematostella
Match: EDO41099 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5D1])

HSP 1 Score: 156.762 bits (395), Expect = 2.729e-43
Identity = 96/257 (37.35%), Postives = 143/257 (55.64%), Query Frame = 3

Query:  213 DEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQA-EQLGIPHLLDVQDVDVAKPDEKSIMTYITQF---LKSYPEGGKEIEENIVATNNAR 971
            DE E  Q+KTFT WIN  L +A    ++ +L+  +KDG  LI LLE +TG+K+++E+  NM ++H L+N+N A+  L+   I+L+NI+  DI DG   + LGL+W+VI  FQ+         AL    D +   +  LL+W   T      ++VT+F  SWRDG AFNA++H   P L  ++ +   +   N + AFK A + LG+  LLD +DV   +PD+KSI+ Y+T     L     G       +  T N R
Sbjct:    2 DEHEGTQKKTFTKWINIQLSRAGNNVRVGDLFNDLKDGTILIALLEVLTGKKIKRERG-NM-RLHHLNNVNNAMMILEENDIRLINISNNDIVDGNHKMTLGLIWSVIAHFQLNF-------ALQFLMDEEGTPEEVLLSWCQNTTKGYKGVSVTNFTTSWRDGLAFNAVIHRYRPDLFSYDALVGSSPMSNCEHAFKVARDSLGVDSLLDAEDVVRDRPDKKSIIMYVTMLCHKLSPLKMGADNGGHGVTVTRNGR 249

[Close]

BLAST of Nesprin-1 vs. Ensembl Medaka
Match: ENSORLT00000000374.2 (pep primary_assembly:ASM223467v1:22:316712:329038:1 gene:ENSORLG00000000306.2 transcript:ENSORLT00000000374.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 239.58 bits (610), Expect = 1.070e-72
Identity = 137/266 (51.50%), Postives = 179/266 (67.29%), Query Frame = 3

Query:  216 EQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGA---------------------------DYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGG 929
            EQE VQ++TFT WIN++L       K+++L+E IKDG+ L+ LLE ++G++L  EQ +++++IH +SNI+TAL+FL+ RKIKLVNI+ATD+ADGRPSI+LGL+WT+IL+FQIEE    L  AL A                            + G  NAK  LL WV  T    + + V DFGPSWRDG AF ++VH I P LVD   V+ ++N+ENL+EAF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLK YP  G
Sbjct:    5 EQEAVQKRTFTKWINSHL------EKMNDLFEDIKDGVKLLALLEVLSGQRLPCEQGRHLKRIHWVSNISTALKFLEGRKIKLVNIHATDVADGRPSIVLGLIWTIILYFQIEELTSNL-PALQAFSNSSSSVDSLTSSETGSPPVKRKVLHKFQG--NAKKVLLRWVQCTAAKHHGIEVKDFGPSWRDGVAFQSMVHAIRPDLVDMEEVRRRSNRENLEEAFSLAENELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHYPNPG 261

[Close]

BLAST of Nesprin-1 vs. Ensembl Medaka
Match: ENSORLT00000028699.1 (pep primary_assembly:ASM223467v1:24:9030419:9045840:-1 gene:ENSORLG00000028886.1 transcript:ENSORLT00000028699.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 226.483 bits (576), Expect = 8.488e-70
Identity = 133/296 (44.93%), Postives = 182/296 (61.49%), Query Frame = 3

Query:  216 EQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRK-------IKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXX----------------------------XXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGK-----EIEENIVATNNARKKQ 980
            EQE VQ++TFT WIN++L +  PP ++ +L+E IKDG+ L+ LLE ++G+KL  EQ K +++IH ++NI TAL+FL+ RK       IKLVNIN+TDI DGRPSI+LGL+WT+IL++QIEE                                    L     G   A   LL WV +T      L V DFGPSWR G AF AL++ + P+LVD   V+ ++N+ENLQEAF  AE +LGIP LLD +DVDV KPDEKS+MTY+ QFLK  P+  +     + +E + +  N R+++
Sbjct:   15 EQEAVQKRTFTKWINSHLAKHAPPLEVKDLFEDIKDGVMLLALLEVLSGQKLPCEQGKKLKRIHWVANIGTALKFLEGRKSAYRGSPIKLVNINSTDIVDGRPSIVLGLVWTIILYYQIEELTSSLPELPLLSSSTSSMDSSTSSTDTTSPPVKRKPLPPVQGGARKA---LLRWVQQTSTKCLRLEVKDFGPSWRTGLAFFALINALRPNLVDMERVRRRSNRENLQEAFLLAETELGIPQLLDPEDVDVDKPDEKSVMTYVAQFLKYPPQRKQRDAQGQQQEEVTSCQNCREQR 307

[Close]

BLAST of Nesprin-1 vs. Ensembl Medaka
Match: ENSORLT00000033742.1 (pep primary_assembly:ASM223467v1:24:9030654:9050235:-1 gene:ENSORLG00000028886.1 transcript:ENSORLT00000033742.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 231.876 bits (590), Expect = 1.371e-69
Identity = 132/288 (45.83%), Postives = 181/288 (62.85%), Query Frame = 3

Query:  219 QERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXX----------------------------XXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGK-----EIEENIVATNNARKKQ 980
            QE VQ++TFT WIN++L +  PP ++ +L+E IKDG+ L+ LLE ++G+KL  EQ K +++IH ++NI TAL+FL+ RKIKLVNIN+TDI DGRPSI+LGL+WT+IL++QIEE                                    L     G   A   LL WV +T      L V DFGPSWR G AF AL++ + P+LVD   V+ ++N+ENLQEAF  AE +LGIP LLD +DVDV KPDEKS+MTY+ QFLK  P+  +     + +E + +  N R+++
Sbjct:   19 QEAVQKRTFTKWINSHLAKHAPPLEVKDLFEDIKDGVMLLALLEVLSGQKLPCEQGKKLKRIHWVANIGTALKFLEGRKIKLVNINSTDIVDGRPSIVLGLVWTIILYYQIEELTSSLPELPLLSSSTSSMDSSTSSTDTTSPPVKRKPLPPVQGGARKA---LLRWVQQTSTKCLRLEVKDFGPSWRTGLAFFALINALRPNLVDMERVRRRSNRENLQEAFLLAETELGIPQLLDPEDVDVDKPDEKSVMTYVAQFLKYPPQRKQRDAQGQQQEEVTSCQNCREQR 303

[Close]

BLAST of Nesprin-1 vs. Ensembl Medaka
Match: ENSORLT00000031933.1 (pep primary_assembly:ASM223467v1:24:9030419:9050235:-1 gene:ENSORLG00000028886.1 transcript:ENSORLT00000031933.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 224.172 bits (570), Expect = 6.225e-69
Identity = 132/295 (44.75%), Postives = 181/295 (61.36%), Query Frame = 3

Query:  219 QERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRK-------IKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXX----------------------------XXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGK-----EIEENIVATNNARKKQ 980
            QE VQ++TFT WIN++L +  PP ++ +L+E IKDG+ L+ LLE ++G+KL  EQ K +++IH ++NI TAL+FL+ RK       IKLVNIN+TDI DGRPSI+LGL+WT+IL++QIEE                                    L     G   A   LL WV +T      L V DFGPSWR G AF AL++ + P+LVD   V+ ++N+ENLQEAF  AE +LGIP LLD +DVDV KPDEKS+MTY+ QFLK  P+  +     + +E + +  N R+++
Sbjct:   19 QEAVQKRTFTKWINSHLAKHAPPLEVKDLFEDIKDGVMLLALLEVLSGQKLPCEQGKKLKRIHWVANIGTALKFLEGRKSAYRGSPIKLVNINSTDIVDGRPSIVLGLVWTIILYYQIEELTSSLPELPLLSSSTSSMDSSTSSTDTTSPPVKRKPLPPVQGGARKA---LLRWVQQTSTKCLRLEVKDFGPSWRTGLAFFALINALRPNLVDMERVRRRSNRENLQEAFLLAETELGIPQLLDPEDVDVDKPDEKSVMTYVAQFLKYPPQRKQRDAQGQQQEEVTSCQNCREQR 310

[Close]

BLAST of Nesprin-1 vs. Ensembl Medaka
Match: ENSORLT00000037920.1 (pep primary_assembly:ASM223467v1:11:16926158:17021095:-1 gene:ENSORLG00000025786.1 transcript:ENSORLT00000037920.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 199.519 bits (506), Expect = 3.095e-58
Identity = 113/266 (42.48%), Postives = 158/266 (59.40%), Query Frame = 3

Query:  165 HDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSY---PEGGKEIEENIV 953
             DSV    E  ++   DE++RVQ+KTFT W+N +L++      I +LYE ++DG  LI LLE ++G  L +E  K   + HRL N+  AL+FLK R++KLVNI   DI DG P + LGL+WT+IL FQI E       A        L AK  LL W  +       L   +F  SW DG+ FNAL+H   P L++   V  ++N++NL++AF+ AE LG+  LLD +DVDV  PDEKS++TY++    ++   PEGG+ I  N V
Sbjct:   59 QDSVLDPAERAVVRVADERDRVQKKTFTKWVNKHLIKVRK--HIADLYEDLRDGHNLISLLEVLSGVALPRE--KGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISEIYVSGESA-------DLTAKEKLLLWSQQATDGYPGLRCVNFTSSWSDGRMFNALLHRYRPDLINMEVVSQQSNRDNLEQAFEIAESLGVTRLLDAEDVDVPSPDEKSVITYVSSIYDAFPKIPEGGEGISANEV 313

[Close]

BLAST of Nesprin-1 vs. Planmine SMEST
Match: SMESG000069851.1 (SMESG000069851.1)

HSP 1 Score: 532.332 bits (1370), Expect = 6.374e-175
Identity = 271/274 (98.91%), Postives = 274/274 (100.00%), Query Frame = 3

Query:  156 MEDHDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGKEIEENIVATNNARKK 977
            MEDHDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVILFFQIEEQEEILIGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGKEIEENIVATNNA+++
Sbjct:    1 MEDHDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRKIKLVNINATDIADGRPSIILGLMWTVILFFQIEEQEEILIGALGADYDGKLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGKEIEENIVATNNAKEE 274

[Close]

BLAST of Nesprin-1 vs. Planmine SMEST
Match: SMESG000077685.1 (SMESG000077685.1)

HSP 1 Score: 260.381 bits (664), Expect = 9.534e-78
Identity = 156/261 (59.77%), Postives = 192/261 (73.56%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKS-RKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDG----KLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGK--EIEENIVATNNA 968
            KDEQE++Q+KTFT W+N+YL Q  PP  I +L + + DGL L+ LLE +T   L+KE+PKNMQKIH LSNI TALEF+++ +KIKLVNIN +D+ DG+P+I+LGL+WT IL FQIEEQEE L+ ALG   D     K  AK  L+ W N  + DKY LN+ DFG SW DG+AFNALVHNIDP+LV+   ++  TNKE L+ AF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLKSYPEG K   I E I A  N+
Sbjct:   15 KDEQEKMQKKTFTKWMNSYLCQKKPPIIIKDLMKDLSDGLLLLDLLEILTKTTLKKEKPKNMQKIHCLSNIRTALEFMENDKKIKLVNINPSDVVDGKPAIVLGLVWTAILHFQIEEQEEFLLQALGMANDPNAKVKGQAKKGLMAWTNNALSDKYGLNIKDFGESWCDGRAFNALVHNIDPTLVNMEEIEKGTNKERLENAFNIAENELGIPKLLDSEDVDVEKPDEKSIMTYVAQFLKSYPEGKKPENISEEITAEINS 275

[Close]

BLAST of Nesprin-1 vs. Planmine SMEST
Match: SMESG000077685.1 (SMESG000077685.1)

HSP 1 Score: 258.455 bits (659), Expect = 4.575e-77
Identity = 155/261 (59.39%), Postives = 192/261 (73.56%), Query Frame = 3

Query:  210 KDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKS-RKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDG----KLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAE-QLGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGK--EIEENIVATNNA 968
            K+EQE++Q+KTFT W+N+YL Q  PP  I +L + + DGL L+ LLE +T   L+KE+PKNMQKIH LSNI TALEF+++ +KIKLVNIN +D+ DG+P+I+LGL+WT IL FQIEEQEE L+ ALG   D     K  AK  L+ W N  + DKY LN+ DFG SW DG+AFNALVHNIDP+LV+   ++  TNKE L+ AF  AE +LGIP LLD +DVDV KPDEKSIMTY+ QFLKSYPEG K   I E I A  N+
Sbjct:   16 KNEQEKMQKKTFTKWMNSYLCQKKPPIIIKDLMKDLSDGLLLLDLLEILTKTTLKKEKPKNMQKIHCLSNIRTALEFMENDKKIKLVNINPSDVVDGKPAIVLGLVWTAILHFQIEEQEEFLLQALGMANDPNAKVKGQAKKGLMAWTNNALSDKYGLNIKDFGESWCDGRAFNALVHNIDPTLVNMEEIEKGTNKERLENAFNIAENELGIPKLLDSEDVDVEKPDEKSIMTYVAQFLKSYPEGKKPENISEEITAEINS 276

[Close]

BLAST of Nesprin-1 vs. Planmine SMEST
Match: SMESG000023320.1 (SMESG000023320.1)

HSP 1 Score: 227.254 bits (578), Expect = 3.224e-66
Identity = 126/270 (46.67%), Postives = 179/270 (66.30%), Query Frame = 3

Query:  189 ETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLK-SRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDG---------KLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQ-LGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPEGGKEIEENIVATNN 965
            E  +   K+E E +Q+KTFT WIN  L + HP  KI NLY  + DG+ L+ LL+ +T +KL  E+ K+MQ++H L+NI + + FLK +  IKL+NI+A DI DGRP+IILGL+W +IL FQI+ +       L A  D          K N + +LL W +K I + Y +++ DF  SW+DGKAFNA++HNI+P+LVDW+ +K + ++ENL+ AF+ AE+ L IP LL+ +DVDV  PDEKSIMTY++ FL +YP+     EE   A N+
Sbjct:    2 ECSVFYNKNEHEHIQKKTFTKWINNLLSKKHPEEKIQNLYVDLCDGIRLLELLQILTKQKLTIEKRKHMQRVHCLANICSLIGFLKDTMHIKLINIHAADIVDGRPNIILGLVWQIILNFQIKSENMF---KLIAQRDSNPTRRENLVKGNPREALLAWSDKVITENYGISINDFHSSWKDGKAFNAIIHNINPNLVDWDLIKRRNSRENLEYAFRLAEKYLNIPKLLEPEDVDVDYPDEKSIMTYLSLFLHNYPDQDIHKEEENKAFND 268

[Close]

BLAST of Nesprin-1 vs. Planmine SMEST
Match: SMESG000023320.1 (SMESG000023320.1)

HSP 1 Score: 226.098 bits (575), Expect = 6.566e-66
Identity = 122/256 (47.66%), Postives = 174/256 (67.97%), Query Frame = 3

Query:  189 ETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNLYESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLK-SRKIKLVNINATDIADGRPSIILGLMWTVXXXXXXXXXXXXXXGALGADYDG---------KLNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDWNGVKNKTNKENLQEAFKQAEQ-LGIPHLLDVQDVDVAKPDEKSIMTYITQFLKSYPE 923
            E  +   K+E E +Q+KTFT WIN  L + HP  KI NLY  + DG+ L+ LL+ +T +KL  E+ K+MQ++H L+NI + + FLK +  IKL+NI+A DI DGRP+IILGL+W +IL FQI+ +       L A  D          K N + +LL W +K I + Y +++ DF  SW+DGKAFNA++HNI+P+LVDW+ +K + ++ENL+ AF+ AE+ L IP LL+ +DVDV  PDEKSIMTY++ FL +YP+
Sbjct:    2 ECSVFYNKNEHEHIQKKTFTKWINNLLSKKHPEEKIQNLYVDLCDGIRLLELLQILTKQKLTIEKRKHMQRVHCLANICSLIGFLKDTMHIKLINIHAADIVDGRPNIILGLVWQIILNFQIKSENMF---KLIAQRDSNPTRRENLVKGNPREALLAWSDKVITENYGISINDFHSSWKDGKAFNAIIHNINPNLVDWDLIKRRNSRENLEYAFRLAEKYLNIPKLLEPEDVDVDYPDEKSIMTYLSLFLHNYPD 254

The following BLAST results are available for this feature:

BLAST of Nesprin-1 vs. Ensembl Human
BLAST of Nesprin-1 vs. Ensembl Celegans
BLAST of Nesprin-1 vs. Ensembl Fly
BLAST of Nesprin-1 vs. Ensembl Zebrafish
BLAST of Nesprin-1 vs. Ensembl Xenopus
BLAST of Nesprin-1 vs. Ensembl Mouse
BLAST of Nesprin-1 vs. UniProt/SwissProt
BLAST of Nesprin-1 vs. TrEMBL
BLAST of Nesprin-1 vs. Ensembl Cavefish
BLAST of Nesprin-1 vs. Ensembl Sea Lamprey
BLAST of Nesprin-1 vs. Ensembl Yeast
BLAST of Nesprin-1 vs. Ensembl Nematostella
BLAST of Nesprin-1 vs. Ensembl Medaka
BLAST of Nesprin-1 vs. Planmine SMEST

BLAST of Nesprin-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
SYNE1	7.258e-77	50.94	spectrin repeat containing nuclear envelope protei...	[more]
SYNE1	1.359e-75	50.94	spectrin repeat containing nuclear envelope protei...	[more]
SYNE1	2.478e-75	47.43	spectrin repeat containing nuclear envelope protei...	[more]
SYNE1	1.252e-74	50.94	spectrin repeat containing nuclear envelope protei...	[more]
SYNE1	1.458e-74	50.94	spectrin repeat containing nuclear envelope protei...	[more]

BLAST of Nesprin-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
unc-70	2.411e-48	40.08	Spectrin beta chain [Source:UniProtKB/TrEMBL;Acc:...	[more]
unc-70	2.417e-48	40.08	Spectrin beta chain [Source:UniProtKB/TrEMBL;Acc:...	[more]
unc-70	2.708e-48	40.08	Spectrin beta chain [Source:UniProtKB/TrEMBL;Acc:...	[more]
unc-70	3.684e-48	42.49	Spectrin beta chain [Source:UniProtKB/TrEMBL;Acc:...	[more]
vab-10	3.640e-47	36.33	VAB-10B protein [Source:UniProtKB/TrEMBL;Acc:G5EF...	[more]

BLAST of Nesprin-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Msp300	6.562e-67	46.50	gene:FBgn0261836 transcript:FBtr0332405	[more]
Msp300	6.626e-67	46.50	gene:FBgn0261836 transcript:FBtr0303385	[more]
Msp300	9.907e-67	46.50	gene:FBgn0261836 transcript:FBtr0303383	[more]
Msp300	4.307e-66	48.29	gene:FBgn0261836 transcript:FBtr0332404	[more]
Msp300	5.071e-66	48.29	gene:FBgn0261836 transcript:FBtr0303388	[more]

BLAST of Nesprin-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
syne1b	1.003e-70	50.37	spectrin repeat containing, nuclear envelope 1b [S...	[more]
syne1b	1.003e-70	50.37	spectrin repeat containing, nuclear envelope 1b [S...	[more]
syne1a	6.830e-62	45.42	spectrin repeat containing, nuclear envelope 1a [S...	[more]
syne1a	9.286e-62	45.42	spectrin repeat containing, nuclear envelope 1a [S...	[more]
syne2b	4.240e-58	40.43	spectrin repeat containing, nuclear envelope 2b [S...	[more]

BLAST of Nesprin-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
SYNE1	9.160e-70	45.83	spectrin repeat containing, nuclear envelope 1 [So...	[more]
SYNE1	1.632e-63	45.91	spectrin repeat containing, nuclear envelope 1 [So...	[more]
SYNE1	1.797e-63	45.91	spectrin repeat containing, nuclear envelope 1 [So...	[more]
SYNE1	2.052e-63	45.91	spectrin repeat containing, nuclear envelope 1 [So...	[more]
SYNE1	2.710e-63	45.66	spectrin repeat containing, nuclear envelope 1 [So...	[more]

BLAST of Nesprin-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Syne1	6.865e-78	49.06	spectrin repeat containing, nuclear envelope 1 [So...	[more]
Syne1	9.807e-73	47.79	spectrin repeat containing, nuclear envelope 1 [So...	[more]
Syne1	7.310e-68	47.79	spectrin repeat containing, nuclear envelope 1 [So...	[more]
Macf1	6.799e-63	43.89	microtubule-actin crosslinking factor 1 [Source:MG...	[more]
Macf1	5.437e-60	42.96	microtubule-actin crosslinking factor 1 [Source:MG...	[more]

BLAST of Nesprin-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q8NF91\|SYNE1_HUMAN	6.012e-74	50.94	Nesprin-1 OS=Homo sapiens OX=9606 GN=SYNE1 PE=1 SV...	[more]
sp\|Q6ZWR6\|SYNE1_MOUSE	2.019e-69	49.06	Nesprin-1 OS=Mus musculus OX=10090 GN=Syne1 PE=1 S...	[more]
sp\|Q9UPN3\|MACF1_HUMAN	4.107e-59	42.96	Microtubule-actin cross-linking factor 1, isoforms...	[more]
sp\|D3ZHV2\|MACF1_RAT	4.129e-59	42.96	Microtubule-actin cross-linking factor 1 OS=Rattus...	[more]
sp\|Q9QXZ0\|MACF1_MOUSE	4.146e-59	42.96	Microtubule-actin cross-linking factor 1 OS=Mus mu...	[more]

BLAST of Nesprin-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0X3P4L6	7.974e-87	59.29	Uncharacterized protein (Fragment) OS=Schistocepha...	[more]
A0A1I8GMD0	2.819e-85	56.36	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A0X3P9Y2	1.369e-84	57.92	Nesprin-1 (Fragment) OS=Schistocephalus solidus OX...	[more]
A0A5K3ELW0	5.326e-82	56.20	Uncharacterized protein OS=Mesocestoides corti OX=...	[more]
A0A2R2MSB1	7.308e-82	54.55	nesprin-1-like OS=Lingula unguis OX=7574 GN=LOC106...	[more]

BLAST of Nesprin-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000036923.1	3.376e-75	47.23	pep primary_assembly:Astyanax_mexicanus-2.0:9:2416...	[more]
ENSAMXT00000039764.1	3.872e-75	47.41	pep primary_assembly:Astyanax_mexicanus-2.0:9:2416...	[more]
ENSAMXT00000045503.1	1.203e-71	47.23	pep primary_assembly:Astyanax_mexicanus-2.0:9:2416...	[more]
syne1a	3.307e-69	49.25	spectrin repeat containing, nuclear envelope 1a [S...	[more]
MACF1	2.353e-59	42.91	microtubule actin crosslinking factor 1 [Source:HG...	[more]

BLAST of Nesprin-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSPMAT00000009426.1	1.087e-63	46.18	pep scaffold:Pmarinus_7.0:GL476614:383620:553344:1...	[more]
ENSPMAT00000002838.1	2.507e-54	43.88	pep scaffold:Pmarinus_7.0:GL477168:22072:87306:1 g...	[more]
ENSPMAT00000006515.1	4.090e-50	40.87	pep scaffold:Pmarinus_7.0:GL476558:286719:335794:-...	[more]
ENSPMAT00000008111.1	3.368e-49	43.97	pep scaffold:Pmarinus_7.0:GL478136:8967:48859:1 ge...	[more]
ENSPMAT00000006506.1	4.202e-49	40.34	pep scaffold:Pmarinus_7.0:GL476558:286440:335800:-...	[more]

BLAST of Nesprin-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
SAC6	6.317e-9	21.83	Fimbrin, actin-bundling protein; cooperates with S...	[more]

BLAST of Nesprin-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO42431	7.161e-48	40.86	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO29101	1.064e-45	36.33	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO37708	4.062e-44	43.70	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO30309	2.144e-43	38.86	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO41099	2.729e-43	37.35	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Nesprin-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000000374.2	1.070e-72	51.50	pep primary_assembly:ASM223467v1:22:316712:329038:...	[more]
ENSORLT00000028699.1	8.488e-70	44.93	pep primary_assembly:ASM223467v1:24:9030419:904584...	[more]
ENSORLT00000033742.1	1.371e-69	45.83	pep primary_assembly:ASM223467v1:24:9030654:905023...	[more]
ENSORLT00000031933.1	6.225e-69	44.75	pep primary_assembly:ASM223467v1:24:9030419:905023...	[more]
ENSORLT00000037920.1	3.095e-58	42.48	pep primary_assembly:ASM223467v1:11:16926158:17021...	[more]

BLAST of Nesprin-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000069851.1	6.374e-175	98.91	SMESG000069851.1	[more]
SMESG000077685.1	9.534e-78	59.77	SMESG000077685.1	[more]
SMESG000077685.1	4.575e-77	59.39	SMESG000077685.1	[more]
SMESG000023320.1	3.224e-66	46.67	SMESG000023320.1	[more]
SMESG000023320.1	6.566e-66	47.66	SMESG000023320.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30013390 ID=SMED30013390|Name=Nesprin-1|organism=Schmidtea mediterranea sexual|type=transcript|length=1052bp
TGAATTGTTGATCGATTCTGCATTTTGATAGTGATCGAAACACAAACTCG
AATTGTATTTGTATAGATTACTTTCTCATCTGTAGCCATAGTTACGAAGC
CCGGCTATTTTAAATTCGATTAATACAAAACAAATTTTATTTCGAATTTT
CAACGATGGAAGATCACGATTCTGTGAAAGGCAAAACTGAAACTTTAATT
CTTTCGAAAAAAGATGAACAAGAAAGGGTACAGAGGAAAACGTTCACCAA
TTGGATCAATACATATCTAGTACAGGCTCATCCTCCGTATAAAATAGACA
ATCTCTATGAAAGTATCAAAGATGGACTTTGTCTCATACACCTGCTAGAG
GCGATGACGGGAGAGAAACTCAGGAAGGAACAACCAAAAAATATGCAAAA
GATTCACAGGCTTTCAAATATAAATACAGCTCTAGAATTTTTGAAAAGTA
GAAAAATAAAACTTGTTAATATCAACGCAACGGACATTGCTGATGGACGG
CCATCAATCATTTTGGGACTAATGTGGACGGTCATTCTATTTTTTCAAAT
TGAAGAACAAGAAGAAATACTGATTGGAGCGTTAGGTGCAGATTATGATG
GGAAATTAAATGCTAAAACTTCTTTATTAAATTGGGTTAATAAGACTATT
GGAGATAAATATAATCTAAACGTCACTGATTTTGGTCCAAGTTGGAGAGA
CGGGAAAGCATTTAATGCACTTGTACACAATATAGACCCCTCACTCGTCG
ACTGGAACGGTGTGAAAAATAAAACTAATAAGGAAAATTTACAGGAAGCT
TTTAAACAAGCTGAGCAATTAGGAATACCTCATCTTTTAGATGTTCAAGA
TGTTGATGTGGCTAAACCTGATGAAAAATCTATTATGACATATATTACAC
AGTTTTTAAAATCCTATCCTGAAGGGGGTAAAGAAATAGAAGAAAATATT
GTTGCAACAAATAATGCAAGGAAGAAGCAGCTTTACGTGCACAAGAAGAA
GAATTAGAACGACAACGACTTGAAGAGGAAGAAAGGCAAAAGCAAGAAGC
TG

protein sequence of SMED30013390-orf-1

>SMED30013390-orf-1 ID=SMED30013390-orf-1|Name=SMED30013390-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=284bp
MEDHDSVKGKTETLILSKKDEQERVQRKTFTNWINTYLVQAHPPYKIDNL
YESIKDGLCLIHLLEAMTGEKLRKEQPKNMQKIHRLSNINTALEFLKSRK
IKLVNINATDIADGRPSIILGLMWTVILFFQIEEQEEILIGALGADYDGK
LNAKTSLLNWVNKTIGDKYNLNVTDFGPSWRDGKAFNALVHNIDPSLVDW
NGVKNKTNKENLQEAFKQAEQLGIPHLLDVQDVDVAKPDEKSIMTYITQF
LKSYPEGGKEIEENIVATNNARKKQLYVHKKKN*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000020	protonephridia
PLANA:0000099	neuron
PLANA:0000418	head

Vocabulary: **INTERPRO**
Term	Definition
IPR036872	CH_dom_sf
IPR001715	CH-domain
IPR001589	Actinin_actin-bd_CS

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding
GO:0003779	actin binding
GO:0005088	Ras guanyl-nucleotide exchange factor activity
GO:0005200	structural constituent of cytoskeleton
GO:0030506	ankyrin binding
GO:0051015	actin filament binding

Vocabulary: **biological process**
Term	Definition
GO:0000165	MAPK cascade
GO:0006888	ER to Golgi vesicle-mediated transport
GO:0007010	cytoskeleton organization
GO:0007411	axon guidance
GO:0043547	positive regulation of GTPase activity
GO:0051693	actin filament capping

Vocabulary: **cellular component**
Term	Definition
GO:0005737	cytoplasm
GO:0005794	Golgi apparatus
GO:0005829	cytosol
GO:0005856	cytoskeleton
GO:0005938	cell cortex
GO:0008091	spectrin
GO:0009986	cell surface
GO:0014731	spectrin-associated cytoskeleton
GO:0015629	actin cytoskeleton
GO:0031235	intrinsic component of the cytoplasmic side of the plasma membrane
GO:0043234	protein complex

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001715	Calponin homology domain	SMART	SM00033	ch_5	coord: 26..129 e-value: 5.8E-20 score: 82.3 coord: 153..250 e-value: 4.7E-16 score: 69.3
IPR001715	Calponin homology domain	PFAM	PF00307	CH	coord: 153..254 e-value: 1.3E-20 score: 73.6 coord: 25..131 e-value: 8.6E-22 score: 77.4
IPR001715	Calponin homology domain	PROSITE	PS50021	CH	coord: 24..131 score: 23.852
IPR001715	Calponin homology domain	PROSITE	PS50021	CH	coord: 151..255 score: 23.699
IPR001715	Calponin homology domain	CDD	cd00014	CH	coord: 25..131 e-value: 1.00712E-18 score: 77.3511
IPR001715	Calponin homology domain	CDD	cd00014	CH	coord: 152..250 e-value: 8.00275E-20 score: 80.4327
IPR036872	CH domain superfamily	GENE3D	G3DSA:1.10.418.10		coord: 149..260 e-value: 7.8E-39 score: 134.0
IPR036872	CH domain superfamily	GENE3D	G3DSA:1.10.418.10		coord: 4..135 e-value: 2.2E-40 score: 139.4
IPR036872	CH domain superfamily	SUPERFAMILY	SSF47576	Calponin-homology domain, CH-domain	coord: 14..251
None	No IPR available	PANTHER	PTHR23167	FAMILY NOT NAMED	coord: 7..134
None	No IPR available	PANTHER	PTHR23167	FAMILY NOT NAMED	coord: 152..280
None	No IPR available	PANTHER	PTHR23167:SF28	CALMIN	coord: 7..134 coord: 152..280
IPR001589	Actinin-type actin-binding domain, conserved site	PROSITE	PS00019	ACTININ_1	coord: 26..35
IPR001589	Actinin-type actin-binding domain, conserved site	PROSITE	PS00020	ACTININ_2	coord: 103..127