netrin 1
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30013386 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 28Note: Hover over icons to view figure legend
Homology
BLAST of netrin 1 vs. Ensembl Human
Match: NTN1 (netrin 1 [Source:HGNC Symbol;Acc:HGNC:8029]) HSP 1 Score: 336.265 bits (861), Expect = 1.240e-105 Identity = 203/638 (31.82%), Postives = 327/638 (51.25%), Query Frame = 2
Query: 197 GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHI------NCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMA---------QAPENRCQHCNNKRKRIR--FKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
G RC+P F N ++STCG+ + V +CH C K H +LTD + +N+T W S+ ++ NV +T S GK FE+ Y+SL P+S+ I KS+D G +W + ++S C + + N + IT+ +Q CT DS S ++AF+T + +H D W++AT+IR+ F+R++ + + + + S F+A++D+ +GGRC+C GHA RC+++ D++ CDC+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +GY+RD KP++H C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ KIP+A + PE+ +C + +++ KKYC+KD + + + ++ G+ +F + + VY+ TS+ G S +WI+ D+ CKCP ++ YL++ +A ++S ++ D+ S+ + W+ TW RR++KF +E +G C+K
Sbjct: 46 GHPRRCIPDFVNAAFGKDV-RVSSTCGRPPARYCVVSERGEERLRSCHLCNASDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIYKSMDYGRTWVPFQFYSTQCRKMY-------NRPHRAPITKQN-EQEAVCT---DSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVA-------------------------FSRLH-------------TFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHAARCVRD-RDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMGKPITHRKACKACDCHPVGAAG-KTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCI------KIPVAPPTTAASSVEEPEDCDSYCKASKGKLKINMKKYCKKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRR----------GDQS---------LWIRSRDIACKCPKIKPLKKYLLL--GNAEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKK 603
BLAST of netrin 1 vs. Ensembl Human
Match: NTN3 (netrin 3 [Source:HGNC Symbol;Acc:HGNC:8030]) HSP 1 Score: 273.092 bits (697), Expect = 7.333e-82 Identity = 185/623 (29.70%), Postives = 271/623 (43.50%), Query Frame = 2
Query: 185 CYSEGKAFR-CVPPFTNIIENMIPDEI--TSTCGQNKKQRICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASDFVNHGD-NVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFT---TNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYH--CDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQA--------PENRCQHCNNKRK--RIRFKKYCRKDAVLLVTMQSR-EQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTY----LVILKLHAIKYLERSELLLDQKSVALPWQKTW 1981
C+ EG A R CVP ++ + E+ +STCG+ R C S + HS LT + W S+ + NV +T GK FE+ ++SL P S+ + KS D+G SW +FS C +G P N + L + PD +LAF+ ++ P +S W++AT++R+ L + T R+ +V+ S +A D+ +GGRC+C GHA RCL +D H CDC+H T G DC C+ + D+ W AT + C C+CN H+++C FN +LY +S ++SGGVC++C HNT GR+CH C +G++RD + LS C C CHP+G+ C+Q QC CK GV GL+CNRC GFQQ++SP+ PCV K P+ P++ HC R RI KK+C+KD + V + +R E G RF + V V+R + + +W+ D C CP L G Y L+ + S+ LPW+ W
Sbjct: 30 CHDEGGAPRGCVP---GLVNAALGREVLASSTCGR-PATRACD-----ASDPRRAHSPALLTSPGGTASPLCWRSESLPRAPLNVTLTVPLGKAFELVFVSLRFC-SAPPASVALLKSQDHGRSWAPLGFFSSHCDLDYGRLPAPANGPAGPGPEALCFPAPL---AQPDGS--------GLLAFSMQDSSPPGLDLDSSPVLQDWVTATDVRVVLTRPST---------------------------------------AGDPRDMEAVVPYS--YAATDLQVGGRCKCNGHASRCL---LDTQGHLICDCRHGTEGPDCGRCKPFYCDRPWQRATARESHACLACSCNGHARRCRFNMELYRLSGRRSGGVCLNCRHNTAGRHCHYCREGFYRDPGRALSDRRACRACDCHPVGAAG-KTCNQTTGQCPCKDGVTGLTCNRCAPGFQQSRSPVAPCV------KTPIPGPTEDSSPVQPQDCDSHCKPARGSYRISLKKFCKKDYAVQVAVGARGEARGAWTRFPVAVLAVFRSGEERARR-------------------GSSALWVPAGDAACGCPRLLPGRRYLLLGGGPGAAAGGAGGRGPGLIAARGSLVLPWRDAW 561
BLAST of netrin 1 vs. Ensembl Human
Match: NTN5 (netrin 5 [Source:HGNC Symbol;Acc:HGNC:25208]) HSP 1 Score: 218.779 bits (556), Expect = 1.038e-62 Identity = 124/346 (35.84%), Postives = 178/346 (51.45%), Query Frame = 2
Query: 1019 GRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVID----LHKIPMAQAPENRCQ-HCN--NKRKRIRFKKYCRKDAVLLVTMQSREQHGEM-ARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCE 2032
GRCQC+GHA RC HC +H+T G CE CR D W AT C C+CN H+++C FN +L+ +S +SGGVC C H+T GR+CH C G+ RD S+P+ C C+CHPIG+ C+Q + QCTCK GV GL+CNRC G+QQ++SP PC + + L P A + + +CQ +CN + R + ++YC++D VL + + E G R ++V VY+ D W+ DL C C LQ G YL++ A+ + + L+LD+ +ALPW+ W R +K+ +E G C
Sbjct: 155 GRCQCHGHAARCAARARPPRCHC--RHHTTGPGCESCRPSHRDWPWRPATPRHPHPCLPCSCNQHARRCRFNSELFRLSGGRSGGVCERCRHHTAGRHCHYCQPGFWRDPSQPIFSRRACRACQCHPIGATG-GTCNQTSGQCTCKLGVTGLTCNRCGPGYQQSRSPRMPCQRIPEATTTLATTPGAYSSDPQCQNYCNMSDTRVHMSLRRYCQQDHVLRAQVLASEAAGPAWQRLAVRVLAVYKQRAQPVRRGDQD-------------------AWVPRADLTCGCLRLQPGTDYLLLGS--AVGDPDPTRLILDRHGLALPWRPRWARPLKRLQQEERAGGCR 476
BLAST of netrin 1 vs. Ensembl Human
Match: LAMC1 (laminin subunit gamma 1 [Source:HGNC Symbol;Acc:HGNC:6492]) HSP 1 Score: 189.504 bits (480), Expect = 2.061e-49 Identity = 139/461 (30.15%), Postives = 208/461 (45.12%), Query Frame = 2
Query: 197 GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDH------INCHSCMKDT----HSINYLTDQHLNNNITFWASDF----VNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFS-HASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQ-TKSPITPC 1531
G+ RC+P F N N+ T+TCG + CV +CH C H +LTD + + T+W S V + ++N+T GK F+I Y+ L P+S I K W + Y+S C ++ F I +Q CT S + L + P+ ++ S W++AT+IR+TL + L + + ++ N P VLK S ++A++D ++GGRC+C GHA C+KN D C+C+HNT G DCE C F D+ W AT SA+EC C+CN S++C F+ +LY + GG C +C+ NTDG +C +C + + R L ++ C C C P+GS+ CD +C+CK GV G C+RCQ GF T++ PC
Sbjct: 45 GRPQRCMPEFVNAAFNVTV-VATNTCG-TPPEEYCVQTGVTGVTKSCHLCDAGQPHLQHGAAFLTDYNNQADTTWWQSQTMLAGVQYPSSINLTLHLGKAFDITYVRLKF-HTSRPESFAIYKRTREDGPWIPYQYYSGSCENTYSKANRGF-------IRTGGDEQQALCTD-EFSDISPLTGGNVAFSTLEGRPSAYNFDNSPVLQEWVTATDIRVTLNR------------------LNTFGDEVF--------------------NDPKVLK-SYYYAISDFAVGGRCKCNGHASECMKNEFDKLV-CNCKHNTYGVDCEKCLPFFNDRPWRRATAESASECLPCDCNGRSQECYFDPELYRSTGH--GGHCTNCQDNTDGAHCERCRENFFR-----LGNNEACSSCHCSPVGSLSTQ-CDSY-GRCSCKPGVMGDKCDRCQPGFHSLTEAGCRPC 445 HSP 2 Score: 65.855 bits (159), Expect = 3.005e-10 Identity = 57/197 (28.93%), Postives = 85/197 (43.15%), Query Frame = 2
Query: 1025 CQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCR-CHPIGSIYPDV---CDQRNSQC-TCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCN 1600
C C GH+ C +P T C+C+ NTAG CE C +G+ + +T ++++C+ C C S C K V VC +C T G+ C C+ GY D PL + CR C +I P+ C++ +C C G C+RC+ GF P+A P ++C+ CN
Sbjct: 724 CACNGHSETC--DP--ETGVCNCRDNTAGPHCEKCSDGY----YGDSTAGTSSDCQPCPCPGGS-SCAVVPKTKEV-------VCTNCPTGTTGKRCELCDDGYFGD---PLGRNGPVRLCRLCQCSDNIDPNAVGNCNRLTGECLKCIYNTAGFYCDRCKDGF----------------FGNPLAPNPADKCKACN 885 HSP 3 Score: 64.3142 bits (155), Expect = 6.865e-10 Identity = 51/159 (32.08%), Postives = 69/159 (43.40%), Query Frame = 2
Query: 1031 CYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSG-----GVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQ 1492
C G CLK C +NTAG C+ C++GF + N A +CK CNCNL Y +QS G C +C + G+ C C+ G++ L C +C CH +GS CD R QC C+ G+ G C RC+
Sbjct: 842 CNRLTGECLK----------CIYNTAGFYCDRCKDGFFGNPLAP---NPADKCKACNCNL-----------YGTMKQQSSCNPVTGQC-ECLPHVTGQDCGACDPGFYN-----LQSGQGCERCDCHALGSTNGQ-CDIRTGQCECQPGITGQHCERCE 969 HSP 4 Score: 56.225 bits (134), Expect = 2.674e-7 Identity = 45/140 (32.14%), Postives = 59/140 (42.14%), Query Frame = 2
Query: 1085 CDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCT-CKTGVGGLSCNRCQKGF 1501
C C G+ CEMC G+ R + + C C CN HS+ C+ GVC +C NT G +C +C+ GY+ D + S C C C P GS V + CT C TG G C C G+
Sbjct: 690 CTCPVGYGGQFCEMCLSGY---RRETPNLGPYSPCVLCACNGHSETCD-----------PETGVC-NCRDNTAGPHCEKCSDGYYGDSTAGTSSD--CQPCPC-PGGSSCAVVPKTKEVVCTNCPTGTTGKRCELCDDGY 811
BLAST of netrin 1 vs. Ensembl Human
Match: LAMA5 (laminin subunit alpha 5 [Source:HGNC Symbol;Acc:HGNC:6485]) HSP 1 Score: 179.874 bits (455), Expect = 4.061e-46 Identity = 120/412 (29.13%), Postives = 187/412 (45.39%), Query Frame = 2
Query: 317 CHSCMKDTHSINYLTDQHLNNNITFWASDFVNHG---DNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFS---KDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHA-SDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRC-LKNPVDN-TYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKL-------YIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPD-VCDQRNSQCTCKTGVGGLSCNRCQKGF 1501
C C + + ++ +W S ++ G + VN+T G++F + Y+ + PD ++ +S+D G ++ W +F+ +DC FG Q ++ +IT D + CT+ S++ L N V++ P + + S + ATN+R LR TN L HL G K P+V + ++++ DISIGGRC C+GHA C K+P D C CQHNT G C+ C GF + W AT NSA EC+ CNC H+ C ++ ++ + Q GGVC+DC+H+T G C +C G++R + PL HVC +C C S + D C+ +C C+ G C+ C +GF
Sbjct: 103 CDICTAANSNKAHPASNAIDGTERWWQSPPLSRGLEYNEVNVTLDLGQVFHVAYVLIKFANSPRPDLWVLERSMDFGRTYQPWQFFASSKRDCLERFGPQTLE-------RITR---DDAAICTT-EYSRIVPLENGEIVVSLVNGRPGAMNFSYSPLLREFTKATNVR--LRFLRTNTLLGHLMG--------------------------------KALRDPTVTR-RYYYSIKDISIGGRCVCHGHADACDAKDPTDPFRLQCTCQHNTCGGTCDRCCPGFNQQPWKPATANSANECQSCNCYGHATDCYYDPEVDRRRASQSLDGTYQGGGVCIDCQHHTTGVNCERCLPGFYRSPNHPLDSPHVCRRCNCE---SDFTDGTCEDLTGRCYCRPNFSGERCDVCAEGF 465 HSP 2 Score: 85.1149 bits (209), Expect = 3.186e-16 Identity = 64/204 (31.37%), Postives = 83/204 (40.69%), Query Frame = 2
Query: 1025 CQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKR-------------WSVATVNSAAEC------KRCNCN--LHSKKCE------FNEKLYIVSNKQ----SG---------------GVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKG 1498
CQC+GH+ RCL + DCQHNT G CE C+ GFV R SV + N A C +C C CE F L + S+ Q SG G C C +T G C C G++ + P + C +C C P G+ + CD + C CK GV G C+RCQ+G
Sbjct: 1864 CQCHGHSDRCLPG---SGVCVDCQHNTEGAHCERCQAGFVSSRDDPSAPCVSCPCPLSVPSNNFAEGCVLRGGRTQCLCKPGYAGASCERCAPGFFGNPLVLGSSCQPCDCSGNGDPNLLFSDCDPLTGACRGCLRHTTGPRCEICAPGFYGNALLPGN----CTRCDCTPCGT---EACDPHSGHCLCKAGVTGRRCDRCQEG 2057 HSP 3 Score: 74.7146 bits (182), Expect = 5.780e-13 Identity = 46/148 (31.08%), Postives = 63/148 (42.57%), Query Frame = 2
Query: 1073 NTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPI---GSIYPDVCDQRN--SQCTCKTGVGGLSCNRCQKGF 1501
N C C + G C+ C GF + C C C+ HS +C GVCVDC+HNT+G +C +C G+ P + C+ C C P+ + + + C R +QC CK G G SC RC GF
Sbjct: 1827 NVELCLCPASYRGDSCQECAPGFYRDVKGLFL----GRCVPCQCHGHSDRC-----------LPGSGVCVDCQHNTEGAHCERCQAGFVSSRDDPSAP---CVSCPC-PLSVPSNNFAEGCVLRGGRTQCLCKPGYAGASCERCAPGF 1955 HSP 4 Score: 72.4034 bits (176), Expect = 2.909e-12 Identity = 58/223 (26.01%), Postives = 82/223 (36.77%), Query Frame = 2
Query: 1025 CQCYGHAGRCLKNP----------VDNTYH-----CDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNC--------------------NLHSKKCEFNEKLYI----------VSNKQS-----GGVCVDCEHNTDGRYCHQCNK--------------GYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGF 1501
C CYGHA C +P +D TY DCQH+T G +CE C GF R ++S C+RCNC N ++C+ + + SN G V+C+ + G + C K G H + P + C C+C G + D CD QC C+ G G +C+RC G+
Sbjct: 359 CNCYGHATDCYYDPEVDRRRASQSLDGTYQGGGVCIDCQHHTTGVNCERCLPGFY--RSPNHPLDSPHVCRRCNCESDFTDGTCEDLTGRCYCRPNFSGERCDVCAEGFTGFPSCYPTPSSSNDTREQVLPAGQIVNCDCSAAGTQGNACRKDPRVGRCLCKPNFQGTHCELCAPGFYGPGCQPCQCSSPG-VADDRCDPDTGQCRCRVGFEGATCDRCAPGY 578 HSP 5 Score: 63.929 bits (154), Expect = 1.172e-9 Identity = 44/159 (27.67%), Postives = 62/159 (38.99%), Query Frame = 2
Query: 1025 CQ-CYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKG 1498
CQ C L D C CQ AG C+ CR G+ + C+ C C+ + +GG+C C G C +C+ G+H S C+ C C GS++ CD R+ QC+C+ V GL C+ C G
Sbjct: 584 CQLCGCSPAGTLPEGCDEAGRCLCQPEFAGPHCDRCRPGY----------HGFPNCQACTCDPRGALDQLC---------GAGGLC-RCRPGYTGTACQECSPGFHGFPS--------CVPCHCSAEGSLHA-ACDPRSGQCSCRPRVTGLRCDTCVPG 713 HSP 6 Score: 60.4622 bits (145), Expect = 1.420e-8 Identity = 50/188 (26.60%), Postives = 65/188 (34.57%), Query Frame = 2
Query: 1016 GGRCQCYGHA-----GRCLKN--------PVDN--------TYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCN------LHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKG 1498
GG+C C+ H RC P D T C C T DC +C+ + C+ CNC+ L C+ + G C C N GR C C+ G+H + C C CH G+ P VCD QC CK V G C++C G
Sbjct: 1454 GGQCPCHAHVIGRDCSRCATGYWGFPNCRPCDCGARLCDELTGQCICPPRTIPPDCLLCQP-------QTFGCHPLVGCEECNCSGPGIQELTDPTCDTDS-----------GQC-KCRPNVTGRRCDTCSPGFH--------GYPRCRPCDCHEAGTA-PGVCDPLTGQCYCKENVQGPKCDQCSLG 1613 HSP 7 Score: 55.8398 bits (133), Expect = 3.537e-7 Identity = 44/172 (25.58%), Postives = 62/172 (36.05%), Query Frame = 2
Query: 1082 HCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHC 1597
HC C+ G+ C+ C+EG + C+ C C ++ E + QSG C T G C +C GY W P C +C+C P G CD +C C G+ G C+ C QQ H++P+ P HC
Sbjct: 2039 HCLCKAGVTGRRCDRCQEGHFG-------FDGCGGCRPCACGPAAEGSE--------CHPQSGQC--HCRPGTMGPQCRECAPGY---WGLP---EQGCRRCQC-PGGR-----CDPHTGRCNCPPGLSGERCDTCS---QQ--------------HQVPVPGGPVGHSIHC 2164
BLAST of netrin 1 vs. Ensembl Celegans
Match: unc-6 (Netrin unc-6 [Source:UniProtKB/Swiss-Prot;Acc:P34710]) HSP 1 Score: 324.709 bits (831), Expect = 1.516e-101 Identity = 202/630 (32.06%), Postives = 314/630 (49.84%), Query Frame = 2
Query: 197 GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICV-----DHI---NCHSCMK----DTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNP---NFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPE--NRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
G+ RCVP F N P + TCG N+ + C D I C +C +H + LTD + N+T W S NV++T S GK FE+ Y+S+ L PDS+ + KS D G +WT + ++S +C R FG P D IT+S +++ S GN V P F N P NF S W++AT+I++ + P + + N+Y + + ++KQ+ F+++ ++++GGRC+C GHA RC+ + + Y CDC+HNTAG +CEMC+ D+ W AT NSA C CNCN H+K+C F+ +L+ +S +SGGVC++C HNT GR CH C G+ RD S P++H C C CHP+GS+ C+Q + QC CK GV G +CNRC KG+QQ++S +TPC+ + + E ++C C KR+ KK+C++D + + + SRE A++++ V V++ T +WI + CKCP L++G YL++ K + ER L+++ ++V + W+ ++ +FS K+ G C +
Sbjct: 42 GRPVRCVPEFINAAFGK-PVIASDTCGTNRPDKYCTVKEGPDGIIREQCDTCDARNHFQSHPASLLTDLNSIGNMTCWVSTPSLSPQNVSLTLSLGKKFELTYVSMHFCSRL-PDSMALYKSADFGKTWTPFQFYSSECRRIFGRDP-------DVSITKSNEQEAVCTASHIMGPGGNRVAFP----FLENRPSAQNF--ENSPVLQDWVTATDIKVVFSR--------------LSPDQAELYGLSNDVNSYGN--ETDDEVKQRY-----------FYSMGELAVGGRCKCNGHASRCIFDKM-GRYTCDCKHNTAGTECEMCKPFHYDRPWGRATANSANSCVACNCNQHAKRCRFDAELFRLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGCHPVGSLGKS-CNQSSGQCVCKPGVTGTTCNRCAKGYQQSRSTVTPCIKIPTKADFIGSSHSEEQDQCSKCRIVPKRLNQKKFCKRDHAVQMVVVSREMVDGWAKYKIVVESVFKRGTENMQRGETS-------------------LWISPQGVICKCPKLRVGRRYLLLGKNDSDH--ERDGLMVNPQTVLVEWEDDIMDKVLRFSKKDKLGQCPE 606
BLAST of netrin 1 vs. Ensembl Celegans
Match: unc-6 (Netrin unc-6 [Source:UniProtKB/Swiss-Prot;Acc:P34710]) HSP 1 Score: 324.709 bits (831), Expect = 1.516e-101 Identity = 202/630 (32.06%), Postives = 314/630 (49.84%), Query Frame = 2
Query: 197 GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICV-----DHI---NCHSCMK----DTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNP---NFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPE--NRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
G+ RCVP F N P + TCG N+ + C D I C +C +H + LTD + N+T W S NV++T S GK FE+ Y+S+ L PDS+ + KS D G +WT + ++S +C R FG P D IT+S +++ S GN V P F N P NF S W++AT+I++ + P + + N+Y + + ++KQ+ F+++ ++++GGRC+C GHA RC+ + + Y CDC+HNTAG +CEMC+ D+ W AT NSA C CNCN H+K+C F+ +L+ +S +SGGVC++C HNT GR CH C G+ RD S P++H C C CHP+GS+ C+Q + QC CK GV G +CNRC KG+QQ++S +TPC+ + + E ++C C KR+ KK+C++D + + + SRE A++++ V V++ T +WI + CKCP L++G YL++ K + ER L+++ ++V + W+ ++ +FS K+ G C +
Sbjct: 42 GRPVRCVPEFINAAFGK-PVIASDTCGTNRPDKYCTVKEGPDGIIREQCDTCDARNHFQSHPASLLTDLNSIGNMTCWVSTPSLSPQNVSLTLSLGKKFELTYVSMHFCSRL-PDSMALYKSADFGKTWTPFQFYSSECRRIFGRDP-------DVSITKSNEQEAVCTASHIMGPGGNRVAFP----FLENRPSAQNF--ENSPVLQDWVTATDIKVVFSR--------------LSPDQAELYGLSNDVNSYGN--ETDDEVKQRY-----------FYSMGELAVGGRCKCNGHASRCIFDKM-GRYTCDCKHNTAGTECEMCKPFHYDRPWGRATANSANSCVACNCNQHAKRCRFDAELFRLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGCHPVGSLGKS-CNQSSGQCVCKPGVTGTTCNRCAKGYQQSRSTVTPCIKIPTKADFIGSSHSEEQDQCSKCRIVPKRLNQKKFCKRDHAVQMVVVSREMVDGWAKYKIVVESVFKRGTENMQRGETS-------------------LWISPQGVICKCPKLRVGRRYLLLGKNDSDH--ERDGLMVNPQTVLVEWEDDIMDKVLRFSKKDKLGQCPE 606
BLAST of netrin 1 vs. Ensembl Celegans
Match: lam-2 (Laminin-like protein lam-2 [Source:UniProtKB/Swiss-Prot;Acc:Q18823]) HSP 1 Score: 186.037 bits (471), Expect = 1.314e-48 Identity = 136/468 (29.06%), Postives = 214/468 (45.73%), Query Frame = 2
Query: 167 GGSNSLCYSEG--KAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHIN------CHSCMKD----THSINYLTDQHLNNNITFWASDFVNHGDN----VNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQ---PMDFNHYSDSKITESTVDQS-LTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPV---DNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGF 1501
G + CY + RCVP F N N+ ++T+TCG + + CV + C +C +H YLTD ++ NN T+W SD + G N+T GK F+I Y+ L I P+S I K + W W ++S C ++G+ P+ + + ++ T+ D S +T +I L PS AF S+ W++A+ IRI+L + T + + +++ P VL+ S ++A++D ++GGRC+C GHA C+ + +N C C+HNT G DC C + D+ W T A EC CNC+ S +C F+++L+ + GG C+DC+ NT G +C QC + W +P ++ C+ C C+ IGS+ CD +C CK GV G C++C GF
Sbjct: 34 GADGNPCYDRATRQPQRCVPDFVNAAFNL-EVQVTNTCGTKRPTKFCVQSGHTGQRSVCETCDDRHEGFSHPAKYLTDFNVGNNETWWQSDTMQEGQQYPTTTNLTLVLGKSFDITYVRLKFISP-RPESFTIYKKTHTDSEWEPWQFYSGSCRATYGLSDRAPILPGNEATAQCTKEFSDISPITGGNIA---FSTLEGRPSAHAF---------EESEVLQKWVTASAIRISLNRMNT------------------FGDEVFK--------------------DPQVLR-SYYYAISDFAVGGRCKCNGHASECVGSSSVDGENRLVCRCEHNTQGADCNECLPFYNDRPWRSGTSVEANECIACNCSQLSNRCYFDQQLFEETGH--GGHCIDCQGNTQGVHCEQCIANH---WRRPGENY--CVACGCNEIGSLSTQ-CDNE-GKCQCKPGVTGRFCDQCLDGF 439 HSP 2 Score: 65.855 bits (159), Expect = 9.761e-11 Identity = 43/133 (32.33%), Postives = 60/133 (45.11%), Query Frame = 2
Query: 1091 CQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRC 1489
C NT G +CE C+ G+ W A + C+ C C + N+ + N+Q G DC N G C QC G++ ++ C +C C P+GS + CD QC CK GV G C+RC
Sbjct: 859 CIFNTHGFNCENCKPGY----WGDALIEPKGNCQSCGCFAAGTRRPNNDYTLLECNQQDGQC--DCLPNVIGIQCDQCAHGFYN-----ITSGLGCQECNCDPLGS-EGNTCDVNTGQCQCKPGVTGQRCDRC 979 HSP 3 Score: 58.5362 bits (140), Expect = 1.863e-8 Identity = 62/246 (25.20%), Postives = 90/246 (36.59%), Query Frame = 2
Query: 830 FLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTY--HCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRC--------HPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVD 1537
F + + + H YF R N I ++ K R + S D L+++++G NP T+ HC+C G+ CE C GF R C +C+C+ HS CE G C+ CEHNT G C +C +GY+ D + C KC C H G + +C + C G G C+ C G+ T CV+
Sbjct: 623 FPTTEAQTYRFRVHADPYFGWYPRINELDFIGILSNITAIKIRGTYS---YKDIGYLSNVNLG-----TAGVAPSAANPKQATWIEHCECLPGFVGQFCESCESGF---RRETKFGGPFNHCIKCDCHNHSNSCE-----------AESGSCI-CEHNTAGDTCERCARGYYGDALQGTEED--CQKCPCPNDGPCILHADGDV---ICTE------CPNGYTGRRCDECSDGYFGNPKDGTECVE 834 HSP 4 Score: 55.8398 bits (133), Expect = 1.262e-7 Identity = 53/212 (25.00%), Postives = 78/212 (36.79%), Query Frame = 2
Query: 1022 RCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCN------LHS---------------KKCEFNEKLYIVSNKQSGGVCVDCE------------------------HNTDGRYCHQCNKGYHRDWSKPL-SHHHVCIKCRCHPIGSIYPD------VCDQRNSQCTCKTGVGGLSCNRCQKGF 1501
+C C+ H+ C + C C+HNTAG CE C G+ + A + +C++C C LH+ ++C+ Y N + G CV+C NT G C C GY W L C C C G+ P+ C+Q++ QC C V G+ C++C GF
Sbjct: 735 KCDCHNHSNSCEAE----SGSCICEHNTAGDTCERCARGY----YGDALQGTEEDCQKCPCPNDGPCILHADGDVICTECPNGYTGRRCDECSDGYF-GNPKDGTECVECACSGNTDPNSIGNCDKITGECKKCIFNTHGFNCENCKPGY---WGDALIEPKGNCQSCGCFAAGTRRPNNDYTLLECNQQDGQCDCLPNVIGIQCDQCAHGF 934
BLAST of netrin 1 vs. Ensembl Celegans
Match: lam-1 (LAMinin related. See also lmb [Source:UniProtKB/TrEMBL;Acc:V6CIX7]) HSP 1 Score: 170.244 bits (430), Expect = 1.824e-43 Identity = 132/466 (28.33%), Postives = 199/466 (42.70%), Query Frame = 2
Query: 263 TSTCGQNKKQRICV-----DHINCHSCMKDT------------HSI-NYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSF-GIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVD-----NTY---------HCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRN--------SQCTCKTGVGGLSCNRCQKGF-QQTKSPITPCV 1534
TSTCG +QR C+ + C C T H I N +T+ + N ++ S+ N NV+I+F F ++ ++ P ++II +S D G +W + YF+ DC SF GI ++D T D + S G +V T NP +D+ ++ + TN+R K T L L Y P + + + ++A+ +I + G C CYGHA RC+ P+D NT C+C HNT G +CE C+ + D W A + EC++CNCN H+ +C F+ +Y S SGGVC DC HNT G+ C QC ++RD + + HVC+ C C GS +C+ +C CKT V G C+RC+ G+ T++ + CV
Sbjct: 55 TSTCGSQGRQRFCIVSHLEEQTKCFYCDSRTEWKPQREPYRLSHRIENVVTEVMDDKNRNWYQSE--NGAQNVSISFDLEAEFHFTHL-IMTFKSFRPAAMIIERSADFGKTWQVYRYFAYDCDSSFPGIPEGPPKKHTDVICTSQYSD-------VAPSTGGEIVYKVISPHIVTENP-----YADEISTLLKITNLRFNFTKLHT--LGDDL--LDYRPEIDEKY----------------------------------YYAIYEIVVRGSCSCYGHASRCI--PIDPHVSPNTVMERADIVHGRCECMHNTEGLNCEKCKAFYNDLPWRPAIGDEKNECRQCNCNRHALRCHFDRAVYESSGFVSGGVCDDCMHNTQGKNCEQCKPFFYRDPRRTIDDPHVCLPCECDKAGSQNKGICEGEEDAERGLVAGKCYCKTNVDGNRCDRCKNGYWNLTETNVDGCV 465 HSP 2 Score: 56.6102 bits (135), Expect = 7.880e-8 Identity = 48/185 (25.95%), Postives = 69/185 (37.30%), Query Frame = 2
Query: 980 ISDFFALADISIGGRCQCYGHAGRCLKNPVDN-TYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPC 1531
+ ++ A + RC C K D + C C N G C+ C E + + S A C+ C C+ + ++ N G C+ GR C QC Y W P + C +C C+P GS C + N C C+ G GG CN C +G+ PC
Sbjct: 1023 VDGYYGDAKLKTCQRCVCNELGTNSTKGACDRVSGQCPCHDNIIGMQCDQCAENHFN-------LASGAGCEACGCDSNGVVLNHEGVPHLQCNIFDGQC--QCKPGRGGRKCDQCEDLY---WGDPTTPDG-CHRCECNPTGSKSLQ-CHRNNGTCECQAGSGGALCNECARGYTGQWPYCNPC 1193 HSP 3 Score: 55.8398 bits (133), Expect = 1.346e-7 Identity = 45/164 (27.44%), Postives = 73/164 (44.51%), Query Frame = 2
Query: 1016 GGRCQCYGHAGRC-LKNPVDNTYH--CDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGS-IYPDVCDQRNSQCTCKTGVGGLSCNRCQK 1495
GG Y HA C L+N +NT C+C+ G+ C C + W + C+RC+CN + + S + G C+ C H+T+G C C GY+ D + C +C C+ +G+ CD+ + QC C + G+ C++C +
Sbjct: 928 GGPTSGYQHADTCYLRNSGNNTQDIVCNCKSGYQGERCGECAQ----NHWG-SPREVGGTCERCDCNGNI------DMAMEGSCDAATGECLKCLHHTEGAQCEHCVDGYYGD-----AKLKTCQRCVCNELGTNSTKGACDRVSGQCPCHDNIIGMQCDQCAE 1075 HSP 4 Score: 53.5286 bits (127), Expect = 5.756e-7 Identity = 44/140 (31.43%), Postives = 56/140 (40.00%), Query Frame = 2
Query: 1109 GKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSI----YPDVCDQRNSQ-------CTCKTGVGGLSCNRCQK 1495
G+ C C+ GF W EC+ C CN H+ C+ QS G C++C T G YC +C GY+ D L C C C P G + D C RNS C CK+G G C C +
Sbjct: 855 GRQCNQCQPGF----WGFP------ECRTCQCNDHANICD-----------QSSGACIECRDLTTGHYCDRCQDGYYGD--PRLGVGIPCKPCPC-PGGPTSGYQHADTCYLRNSGNNTQDIVCNCKSGYQGERCGECAQ 970 HSP 5 Score: 49.6766 bits (117), Expect = 9.333e-6 Identity = 38/116 (32.76%), Postives = 55/116 (47.41%), Query Frame = 2
Query: 1157 SVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKT-GVGGLSCNRCQKGF 1501
+ A +N +EC C+ + + N Q GG C +C+ N GR C QC G + C KC C +GS+ D CD+++ QC C+ G+ G CN+CQ GF
Sbjct: 769 AAALLNKTSECN----------CDATGSVSGICNVQ-GGQC-ECKPNVVGRRCDQCAIGTYGFGPSG------CKKCDCDAVGSLGND-CDKQSGQCVCREKGIYGRQCNQCQPGF 865
BLAST of netrin 1 vs. Ensembl Celegans
Match: epi-1 (Laminin-like protein epi-1 [Source:UniProtKB/Swiss-Prot;Acc:Q21313]) HSP 1 Score: 148.288 bits (373), Expect = 2.795e-36 Identity = 127/468 (27.14%), Postives = 199/468 (42.52%), Query Frame = 2
Query: 317 CHSCMKDTHSINYLTDQHLNNNITFWASDFVNHG---DNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSK---DCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHA-SDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRC--LKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQS---------GGVCVDCEHNTDGRYCHQCNKGYHR----DWSKPL----------SHHHVCI----KCRCHP--------------------------IGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCV 1534
C++ +++H + D N ++W S ++ G + VNIT + F + Y+ + + P S ++ +S D+G ++ W F++ +C R FG++ + S I+E D S+TC + S L L N+ V+ + P+ A S+ ++ ATN+R+ L + T L HL + + P+V + F+A+ +I IGGRC C GHA C L+ + C C+HNT G CE C GFV K+W AT ++ C+ CNC S +CE++ ++ + NKQS GGVC +C NT+G C++C+ GY R W++P H C KC C P + DVC + QC CK G GG C C G+ + CV
Sbjct: 104 CNAGNENSHPAANMVD----GNNSWWMSPPLSRGLQHNEVNITIDLEQEFHVAYVWIQMANSPRPGSWVLERSTDHGKTYQPWFNFAENAAECMRRFGMESL-------SPISE---DDSVTCRTDMAS-LQPLENAEMVIRILEHRPSSRQFATSEALQNFTRATNVRLRLLGTRT--LQGHL-------------------------------MDMNEWRDPTVTRRY-FYAIKEIMIGGRCVCNGHAVTCDILEPQRPKSLLCRCEHNTCGDMCERCCPGFVQKQWQAATAHNNFTCEACNCFGRSNECEYDAEVDL--NKQSIDSQGNYEGGGVCKNCRENTEGVNCNKCSFGYFRPEGVTWNEPQPCKVCDCDPDKHTGACAEETGKCECLPRFVGEDCDQCASGYYDAPKCKPCECNVNGTIGDVCLPEDGQCPCKAGFGGTFCETCADGYTNVTAGCVECV 520 HSP 2 Score: 67.3958 bits (163), Expect = 4.246e-11 Identity = 47/160 (29.38%), Postives = 68/160 (42.50%), Query Frame = 2
Query: 1082 HCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSI--YPDVCDQRNS----QCTCKTGVGGLSCNRCQKGF----QQTKSPITPC 1531
C C G C++C G+ V + + C C CN HS C+ + G+C DCEHNT+G +C CN+G++ + + + C+ C C P + CD QC CK G G C+RC GF Q + +PC
Sbjct: 1807 QCQCPAPYTGPSCQLCASGY----HRVQSGSFLGACVPCECNGHSATCDPDT-----------GICTDCEHNTNGDHCEFCNEGHYGNATN--GSPYDCMACAC-PFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSPC 1948 HSP 3 Score: 65.855 bits (159), Expect = 1.063e-10 Identity = 58/207 (28.02%), Postives = 79/207 (38.16%), Query Frame = 2
Query: 1025 CQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNC-----NLHSKKCEFNEKLYIV----------------------------------------------SNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQ 1492
C+C GH+ C +P D DC+HNT G CE C EG + AT S +C C C N +K C+ +E+ ++ S + G C CE NTDGR+C C ++ D + C C C GS Y CD ++ C CK V G SC+RC+
Sbjct: 1841 CECNGHSATC--DP-DTGICTDCEHNTNGDHCEFCNEG----HYGNATNGSPYDCMACACPFAPTNNFAKSCDVSEEGQLLQCNCKPGYTGDRCDRCASGFFGHPQISGESCSPCQCNGNNNLTDSRSCHPNSGDCYLCEQNTDGRHCESCAAWFYGDAVTAKN----CSSCECSQCGSQY---CDNKSGGCECKINVEGDSCDRCK 2033 HSP 4 Score: 57.7658 bits (138), Expect = 3.296e-8 Identity = 42/150 (28.00%), Postives = 60/150 (40.00%), Query Frame = 2
Query: 1070 DNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYP-DVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKS 1516
D+ C C+ N G+ C+ C+ F + C+ CNCN +F +K S G C + GR C QC + W H C C C+ G+I + CD + QC CK G C++C GF + S
Sbjct: 669 DSNGQCYCKGNFEGERCDRCKPNFYNFPI----------CEECNCNPSGVTRDFQG-----CDKVSPGELCSCRKHVTGRICDQCKPTF---WDLQYHHEDGCRSCDCNVNGTISGLNTCDLKTGQCMCKKNADGRRCDQCADGFYRLNS 800 HSP 5 Score: 55.4546 bits (132), Expect = 1.637e-7 Identity = 43/142 (30.28%), Postives = 63/142 (44.37%), Query Frame = 2
Query: 1082 HCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQC-NKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPD-VCDQRNSQCTCKTGVGGLSCNRCQKGF 1501
C C+ G+ CE C G+ + EC +C CN ++C+ + G C C + +G+ C +C + + D PL C KC CHP GS + VCD + QC C+ +GG C+RC GF
Sbjct: 1444 QCKCKPGVIGRRCERCAPGYYN----------FPECIKCQCNA-GQQCD-----------ERTGQCF-CPPHVEGQTCDRCVSNAFGYD---PLIG---CQKCGCHPQGSEGGNLVCDPESGQCLCRESMGGRQCDRCLAGF 1556 HSP 6 Score: 53.9138 bits (128), Expect = 4.764e-7 Identity = 39/134 (29.10%), Postives = 61/134 (45.52%), Query Frame = 2
Query: 1085 CDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNR 1486
C C+ + G+ C+ C+ F D ++ + C+ C+CN++ N + G C+ C+ N DGR C QC G++R S + C C C IG CD + QC C+ V GL C++
Sbjct: 724 CSCRKHVTGRICDQCKPTFWDLQY-----HHEDGCRSCDCNVNGTISGLN------TCDLKTGQCM-CKKNADGRRCDQCADGFYRLNSY---NQMGCESCHCD-IGGALRAECDITSGQCKCRPRVTGLRCDQ 841 HSP 7 Score: 53.5286 bits (127), Expect = 6.410e-7 Identity = 52/198 (26.26%), Postives = 67/198 (33.84%), Query Frame = 2
Query: 1040 HAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVD------CEHNTDGRYCHQCNKGY------------------HRDWS--------KPLSHHHVCIKCR------------CHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGF 1501
H G C + T C+C G+DC+ C G+ D A +CK C CN+ N G VC+ C+ G +C C GY H + S KP C KC+ C P+G+ VCDQ QC CK G G C+RC F
Sbjct: 434 HTGAC----AEETGKCECLPRFVGEDCDQCASGYYD----------APKCKPCECNV---------------NGTIGDVCLPEDGQCPCKAGFGGTFCETCADGYTNVTAGCVECVCDATGSEHGNCSASTGQCECKPAYAGLSCDKCQVGYFGDDCKFCNCDPMGT-EGGVCDQTTGQCLCKEGFAGDKCDRCDIAF 601
BLAST of netrin 1 vs. Ensembl Fly
Match: NetA (gene:FBgn0015773 transcript:FBtr0073942) HSP 1 Score: 307.76 bits (787), Expect = 8.300e-94 Identity = 216/715 (30.21%), Postives = 325/715 (45.45%), Query Frame = 2
Query: 185 CYSEGKAFR-CVPPFTNIIENMIPDEITSTCGQNKKQRICV--DH-INCHSC-MKD---THSINYLTDQHLNNNITFWASDFV-NHGDNVNITFSFGKLFEIYYISLVLIP-LLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQP-MDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSH---ASDQYNSWMSATNIRITL----RKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSIL---PVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPC-------VDVIDLHKI-PMAQAPEN---------------------------RCQHCNNKRKRIRFKKYCRKDAVLLV--------------TMQSREQHGEMARFEM----------KVNYVYRIDTSKFTEMHPD----------------FLASMNG---------IMSNQFIHSTFPMWIKET-DLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNK 2011
CY++ R C+P F N + P +STCG + QR C DH +CH+C M D + LTD + +NN+T W S+ V GDNV +T S GK FE+ Y+ L L P PDS++I KS D+G SW + +FS C R FG +++ + S V + L S +AF+T S +S W++AT+IR+ R L+ G T YS L P ++ + +A++D S+GGRC+C GHA +C + +C+C HNTAG+DCE C+ D+ W+ AT A ECK CNCN H+++C FN +++ +S SGGVC +C H+T GR CHQC +G++RD +KPL+H VC C CHPIGS +C+ + QC CK GV GL+CNRC +G+QQ++S I PC ++++D P PE+ C C KR+ K+C++D ++ +Q R ++A +EM + VY + + +P+ F S G M N + WI T DL+C+CP +++ +YL++ + L + S+ + W++ W RRMK+F +
Sbjct: 40 CYNKAHEPRACIPDFVNAAYDA-PVVASSTCGSSGAQRYCEYQDHERSCHTCDMTDPLRSFPARSLTDLNNSNNVTCWRSEPVTGSGDNVTLTLSLGKKFELTYVILQLCPHAPRPDSMVIYKSTDHGLSWQPFQFFSSQCRRLFGRPARQSTGRHNEHEARCSDVTRPLV----------------SRIAFSTLEGRPSSRDLDSSPVLQDWVTATDIRVVFHRLQRPDPQALLSLEAGGAT-----------DLASGKYSVPLANGPAGNNIEANLGGDVATSGSGLHYAISDFSVGGRCKCNGHASKCSTD-ASGQLNCECSHNTAGRDCERCKPFHFDRPWARATAKEANECKECNCNKHARQCRFNMEIFRLSQGVSGGVCQNCRHSTTGRNCHQCKEGFYRDATKPLTHRKVCKACDCHPIGSSG-KICNSTSGQCPCKDGVTGLTCNRCARGYQQSRSHIAPCIKQPPRMINMLDTQNTAPEPDEPESSPGSGGDRNGAAGMAAQSQYYRTEGGRECGKCRVSTKRLNLNKFCKRDYAIMAKVIGRDTSSEAVSREVQRRAMDPDVADYEMDQVQPGSARSPITGVYEFQAADYPNPNPNPRGSEMERFDLQIQAVFKRSRPGESSGAGNVYGMPNTTLKRGPMTWIIPTKDLECRCPRIRVNRSYLILGRDSEAPP---GYLGIGPHSIVIEWKEDWYRRMKRFQRR 721
BLAST of netrin 1 vs. Ensembl Fly
Match: NetA (gene:FBgn0015773 transcript:FBtr0346088) HSP 1 Score: 307.76 bits (787), Expect = 8.300e-94 Identity = 216/715 (30.21%), Postives = 325/715 (45.45%), Query Frame = 2
Query: 185 CYSEGKAFR-CVPPFTNIIENMIPDEITSTCGQNKKQRICV--DH-INCHSC-MKD---THSINYLTDQHLNNNITFWASDFV-NHGDNVNITFSFGKLFEIYYISLVLIP-LLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQP-MDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSH---ASDQYNSWMSATNIRITL----RKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSIL---PVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPC-------VDVIDLHKI-PMAQAPEN---------------------------RCQHCNNKRKRIRFKKYCRKDAVLLV--------------TMQSREQHGEMARFEM----------KVNYVYRIDTSKFTEMHPD----------------FLASMNG---------IMSNQFIHSTFPMWIKET-DLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNK 2011
CY++ R C+P F N + P +STCG + QR C DH +CH+C M D + LTD + +NN+T W S+ V GDNV +T S GK FE+ Y+ L L P PDS++I KS D+G SW + +FS C R FG +++ + S V + L S +AF+T S +S W++AT+IR+ R L+ G T YS L P ++ + +A++D S+GGRC+C GHA +C + +C+C HNTAG+DCE C+ D+ W+ AT A ECK CNCN H+++C FN +++ +S SGGVC +C H+T GR CHQC +G++RD +KPL+H VC C CHPIGS +C+ + QC CK GV GL+CNRC +G+QQ++S I PC ++++D P PE+ C C KR+ K+C++D ++ +Q R ++A +EM + VY + + +P+ F S G M N + WI T DL+C+CP +++ +YL++ + L + S+ + W++ W RRMK+F +
Sbjct: 40 CYNKAHEPRACIPDFVNAAYDA-PVVASSTCGSSGAQRYCEYQDHERSCHTCDMTDPLRSFPARSLTDLNNSNNVTCWRSEPVTGSGDNVTLTLSLGKKFELTYVILQLCPHAPRPDSMVIYKSTDHGLSWQPFQFFSSQCRRLFGRPARQSTGRHNEHEARCSDVTRPLV----------------SRIAFSTLEGRPSSRDLDSSPVLQDWVTATDIRVVFHRLQRPDPQALLSLEAGGAT-----------DLASGKYSVPLANGPAGNNIEANLGGDVATSGSGLHYAISDFSVGGRCKCNGHASKCSTD-ASGQLNCECSHNTAGRDCERCKPFHFDRPWARATAKEANECKECNCNKHARQCRFNMEIFRLSQGVSGGVCQNCRHSTTGRNCHQCKEGFYRDATKPLTHRKVCKACDCHPIGSSG-KICNSTSGQCPCKDGVTGLTCNRCARGYQQSRSHIAPCIKQPPRMINMLDTQNTAPEPDEPESSPGSGGDRNGAAGMAAQSQYYRTEGGRECGKCRVSTKRLNLNKFCKRDYAIMAKVIGRDTSSEAVSREVQRRAMDPDVADYEMDQVQPGSARSPITGVYEFQAADYPNPNPNPRGSEMERFDLQIQAVFKRSRPGESSGAGNVYGMPNTTLKRGPMTWIIPTKDLECRCPRIRVNRSYLILGRDSEAPP---GYLGIGPHSIVIEWKEDWYRRMKRFQRR 721
BLAST of netrin 1 vs. Ensembl Fly
Match: NetA (gene:FBgn0015773 transcript:FBtr0308337) HSP 1 Score: 280.796 bits (717), Expect = 2.671e-84 Identity = 173/471 (36.73%), Postives = 245/471 (52.02%), Query Frame = 2
Query: 185 CYSEGKAFR-CVPPFTNIIENMIPDEITSTCGQNKKQRICV--DH-INCHSC-MKD---THSINYLTDQHLNNNITFWASDFV-NHGDNVNITFSFGKLFEIYYISLVLIP-LLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQP-MDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITL----RKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSIL---PVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCV 1534
CY++ R C+P F N + P +STCG + QR C DH +CH+C M D + LTD + +NN+T W S+ V GDNV +T S GK FE+ Y+ L L P PDS++I KS D+G SW + +FS C R FG +++ + S V + L S +AF+T S D W++AT+IR+ R L+ G T YS L P ++ + +A++D S+GGRC+C GHA +C + +C+C HNTAG+DCE C+ D+ W+ AT A ECK CNCN H+++C FN +++ +S SGGVC +C H+T GR CHQC +G++RD +KPL+H VC C CHPIGS +C+ + QC CK GV GL+CNRC +G+QQ++S I PC+
Sbjct: 40 CYNKAHEPRACIPDFVNAAYD-APVVASSTCGSSGAQRYCEYQDHERSCHTCDMTDPLRSFPARSLTDLNNSNNVTCWRSEPVTGSGDNVTLTLSLGKKFELTYVILQLCPHAPRPDSMVIYKSTDHGLSWQPFQFFSSQCRRLFGRPARQSTGRHNEHEARCSDVTRPLV----------------SRIAFSTLEGRPSSRDLDSSPVLQDWVTATDIRVVFHRLQRPDPQALLSLEAGGAT-----------DLASGKYSVPLANGPAGNNIEANLGGDVATSGSGLHYAISDFSVGGRCKCNGHASKCSTD-ASGQLNCECSHNTAGRDCERCKPFHFDRPWARATAKEANECKECNCNKHARQCRFNMEIFRLSQGVSGGVCQNCRHSTTGRNCHQCKEGFYRDATKPLTHRKVCKACDCHPIGSSG-KICNSTSGQCPCKDGVTGLTCNRCARGYQQSRSHIAPCI 480
BLAST of netrin 1 vs. Ensembl Fly
Match: NetB (gene:FBgn0015774 transcript:FBtr0304875) HSP 1 Score: 230.72 bits (587), Expect = 7.144e-65 Identity = 129/416 (31.01%), Postives = 201/416 (48.32%), Query Frame = 2
Query: 971 VLKISDFFALADISIGGRCQCYGHA------------------------------------GRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAP----------------------ENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGE---MARFEMKVN---YVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILK--LHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQ 2020
L IS +A++D ++GGRC+C GHA GR + T C C+HNTAG +CE C+ + D+ W AT N A ECK C CN H+++C FN +LY +S + SGGVC +C+H+T GRYCH C +GY+RD +KP +H VC +C CHP+GS C+ + QC CK GV GL+CNRC +G+QQT+S + PC+ V + A++ + C C K++ K+C +D +L + ++ + +F ++ Y Y I+ + +P M+G S+ + + ++C+CP ++L +YL++ + A Y L + SV L W+ W RMK+F + +
Sbjct: 387 TLPISQHYAVSDFAVGGRCKCNGHASECVATVSSGSGTALSDQDDGQDEDTPSAPSLANHFGRSTQMSAKLTMTCACKHNTAGPECERCKPFYFDRPWGRATDNDANECKMCQCNGHARRCRFNLELYKLSGRVSGGVCYNCQHDTTGRYCHYCREGYYRDATKPPNHRKVCKRCDCHPVGSTG-KTCNHLSGQCPCKEGVTGLTCNRCARGYQQTRSHVAPCIKVPTNANMIQAESAGGGGGGGTGDYKDGGGSQVEEMKKYCGKCKASPKKLNLNKFCMEDYAILAKVIGHDRASQDISTEKFSIERQNEIYKYEINIQTIFKRNP-----MSGTTSSLLGRGNMMLLVPRKSIECQCPKIKLNKSYLILGRDSEAAPGY-----LAIGPSSVVLEWKDEWSLRMKRFQRRARK 791 HSP 2 Score: 89.7373 bits (221), Expect = 3.807e-18 Identity = 68/234 (29.06%), Postives = 104/234 (44.44%), Query Frame = 2
Query: 173 SNSLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICV-----DHINCHSCMKDTHSINYLTDQHLNNNITFWASDFVN--------HGDNVNITFSFGKLFEIYYISLVLIPL-LEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSE 823
SN CY EGK +C+P F N P + +S CG + +R C + C SC + + LTD + +N+T W S VN DNV +T S GK +E+ YISL P PDS+ I KS D G +W + ++S C + +G +KI++ ++ S D+ + AF T ++ D W++AT+IR+ + E
Sbjct: 30 SNDPCYFEGKPRKCLPSFVNAAYGN-PVQASSVCGAQQPERYCELLRDGNAGECRSCEQQRYGPAALTDLNNPSNVTCWRSGAVNVPHDPDSAPPDNVTLTLSLGKKYELTYISLSFCPRSPRPDSLAIFKSSDFGQTWQPFQFYSSQCQKFYG-------RPDRAKISKFNEQEARCINSQHDTG-----GAAQRFAFNTLEGRPSANDLDSSLVLQDWVTATDIRVVFHRLE 250
BLAST of netrin 1 vs. Ensembl Fly
Match: NetB (gene:FBgn0015774 transcript:FBtr0302018) HSP 1 Score: 230.72 bits (587), Expect = 7.144e-65 Identity = 129/416 (31.01%), Postives = 201/416 (48.32%), Query Frame = 2
Query: 971 VLKISDFFALADISIGGRCQCYGHA------------------------------------GRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAP----------------------ENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGE---MARFEMKVN---YVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILK--LHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQ 2020
L IS +A++D ++GGRC+C GHA GR + T C C+HNTAG +CE C+ + D+ W AT N A ECK C CN H+++C FN +LY +S + SGGVC +C+H+T GRYCH C +GY+RD +KP +H VC +C CHP+GS C+ + QC CK GV GL+CNRC +G+QQT+S + PC+ V + A++ + C C K++ K+C +D +L + ++ + +F ++ Y Y I+ + +P M+G S+ + + ++C+CP ++L +YL++ + A Y L + SV L W+ W RMK+F + +
Sbjct: 387 TLPISQHYAVSDFAVGGRCKCNGHASECVATVSSGSGTALSDQDDGQDEDTPSAPSLANHFGRSTQMSAKLTMTCACKHNTAGPECERCKPFYFDRPWGRATDNDANECKMCQCNGHARRCRFNLELYKLSGRVSGGVCYNCQHDTTGRYCHYCREGYYRDATKPPNHRKVCKRCDCHPVGSTG-KTCNHLSGQCPCKEGVTGLTCNRCARGYQQTRSHVAPCIKVPTNANMIQAESAGGGGGGGTGDYKDGGGSQVEEMKKYCGKCKASPKKLNLNKFCMEDYAILAKVIGHDRASQDISTEKFSIERQNEIYKYEINIQTIFKRNP-----MSGTTSSLLGRGNMMLLVPRKSIECQCPKIKLNKSYLILGRDSEAAPGY-----LAIGPSSVVLEWKDEWSLRMKRFQRRARK 791 HSP 2 Score: 89.7373 bits (221), Expect = 3.807e-18 Identity = 68/234 (29.06%), Postives = 104/234 (44.44%), Query Frame = 2
Query: 173 SNSLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICV-----DHINCHSCMKDTHSINYLTDQHLNNNITFWASDFVN--------HGDNVNITFSFGKLFEIYYISLVLIPL-LEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSE 823
SN CY EGK +C+P F N P + +S CG + +R C + C SC + + LTD + +N+T W S VN DNV +T S GK +E+ YISL P PDS+ I KS D G +W + ++S C + +G +KI++ ++ S D+ + AF T ++ D W++AT+IR+ + E
Sbjct: 30 SNDPCYFEGKPRKCLPSFVNAAYGN-PVQASSVCGAQQPERYCELLRDGNAGECRSCEQQRYGPAALTDLNNPSNVTCWRSGAVNVPHDPDSAPPDNVTLTLSLGKKYELTYISLSFCPRSPRPDSLAIFKSSDFGQTWQPFQFYSSQCQKFYG-------RPDRAKISKFNEQEARCINSQHDTG-----GAAQRFAFNTLEGRPSANDLDSSLVLQDWVTATDIRVVFHRLE 250
BLAST of netrin 1 vs. Ensembl Zebrafish
Match: ntn1a (netrin 1a [Source:ZFIN;Acc:ZDB-GENE-990415-169]) HSP 1 Score: 350.517 bits (898), Expect = 2.703e-111 Identity = 216/646 (33.44%), Postives = 332/646 (51.39%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNIIENMIPDEI--TSTCGQNKKQRICV-------DHINCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMA---------QAPENRCQHCNNKRKRIR--FKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
CY E G RC+P F N E+ +STCG+ R CV H NCH+C K H YLTD + +N+T W SD ++ + NV +T S GK FE+ Y+SL P+S+ I KS+D G SW + Y+S C + + N S + IT+ +Q CT DS S ++AF+T + +H D W++AT+I++T F+R++ + + + + S F+A++D+ +GGRC+C GHA RC+K+ D C+C+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +GY+RD SKP+SH C C CHP+G+ C+Q QC CK GV G++CNRC G+QQ++SPI PC+ KIP+A + P + +C + +++ KKYC+KD + V + ++ GE +F + + VY+ S+ +QF +W++ D+ CKCP ++ G YL++ +S ++ D+ S+ + W+ TW RR++KF +E +G C+K
Sbjct: 40 CYDENGHPRRCIPDFVNA---AFGKEVRASSTCGKTP-SRYCVVTEKGDERHRNCHTCDASDPKKNHPPAYLTDLNNPHNLTCWQSDNYLQYPQNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIFKSMDYGKSWVPFQYYSTQCRKMY-------NKPSKATITKQN-EQEAICT---DSHTDMHPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVT-------------------------FSRLH-------------TFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASRCVKD-RDGNLVCECKHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMSKPISHRKACKACDCHPVGAA-GKTCNQTTGQCPCKDGVTGITCNRCANGYQQSRSPIAPCI------KIPIAPPTTTASSTEEPSDCESYCKASKGKLKINMKKYCKKDYAVQVHILKADKAGEWWKFTVNIISVYKQGESRIRR-------------GDQF------LWVRAKDVACKCPKIKSGKKYLLLGNDE--DSPGQSGMVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 602
BLAST of netrin 1 vs. Ensembl Zebrafish
Match: ntn1a (netrin 1a [Source:ZFIN;Acc:ZDB-GENE-990415-169]) HSP 1 Score: 350.517 bits (898), Expect = 2.703e-111 Identity = 216/646 (33.44%), Postives = 332/646 (51.39%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNIIENMIPDEI--TSTCGQNKKQRICV-------DHINCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMA---------QAPENRCQHCNNKRKRIR--FKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
CY E G RC+P F N E+ +STCG+ R CV H NCH+C K H YLTD + +N+T W SD ++ + NV +T S GK FE+ Y+SL P+S+ I KS+D G SW + Y+S C + + N S + IT+ +Q CT DS S ++AF+T + +H D W++AT+I++T F+R++ + + + + S F+A++D+ +GGRC+C GHA RC+K+ D C+C+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +GY+RD SKP+SH C C CHP+G+ C+Q QC CK GV G++CNRC G+QQ++SPI PC+ KIP+A + P + +C + +++ KKYC+KD + V + ++ GE +F + + VY+ S+ +QF +W++ D+ CKCP ++ G YL++ +S ++ D+ S+ + W+ TW RR++KF +E +G C+K
Sbjct: 40 CYDENGHPRRCIPDFVNA---AFGKEVRASSTCGKTP-SRYCVVTEKGDERHRNCHTCDASDPKKNHPPAYLTDLNNPHNLTCWQSDNYLQYPQNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIFKSMDYGKSWVPFQYYSTQCRKMY-------NKPSKATITKQN-EQEAICT---DSHTDMHPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVT-------------------------FSRLH-------------TFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASRCVKD-RDGNLVCECKHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMSKPISHRKACKACDCHPVGAA-GKTCNQTTGQCPCKDGVTGITCNRCANGYQQSRSPIAPCI------KIPIAPPTTTASSTEEPSDCESYCKASKGKLKINMKKYCKKDYAVQVHILKADKAGEWWKFTVNIISVYKQGESRIRR-------------GDQF------LWVRAKDVACKCPKIKSGKKYLLLGNDE--DSPGQSGMVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 602
BLAST of netrin 1 vs. Ensembl Zebrafish
Match: ntn2 (netrin 2 [Source:ZFIN;Acc:ZDB-GENE-050310-2]) HSP 1 Score: 335.109 bits (858), Expect = 2.019e-105 Identity = 201/640 (31.41%), Postives = 317/640 (49.53%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICV----DHINCHSCM-------KDTHSINYLTDQHLNNNITFWASDFVNHGD-NVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTT-----NNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHK---IPMAQAPENRCQHCNNKRK--RIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
CY E G A RC+P F N ++S CG+ + + D +C ++ H +YLTD + +N+T W S+ + NV +T S GK FEI Y+SL P+S+ I KS+D G +WT + ++S C R + N + + IT+ ++L CT D S ++AF+T + +F S S W++ T+IR+ F+ P H + LP++ + + +P L + ++A+ D +GGRC+C GHA RC++ D C+C+HNT G +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +G++RD K ++H C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SP+ PC+ + + I + P + +C + +I KKYC+KD + V++ E G+ A+F + + VY+ + +W+ DL CKCP + +G +L++ ER L D+ S+ + W+ W RR++KF KE +G C K
Sbjct: 26 CYEETGAARRCIPEFINAAFGQ-EVSVSSVCGRPVSRSCSLVERDDRPAVRTCQICDASDPRNAHPASYLTDLNSAHNLTCWQSENLQSSPLNVTLTLSLGKKFEITYVSLQFCSP-RPESLAIYKSMDYGRTWTPYQFYSSQCRRMY-------NRPNKAAITKQNEQEAL-CT---DGHTDLYPLSGGLIAFSTLDGRPSGKDFDS--SPVLQDWVTVTDIRVV---------------FSRPQHTR--------------ALPMSGRESEDALGAPGSLP-TYYYAVGDFQVGGRCKCNGHASRCVRE-KDGKLLCECKHNTEGPECDRCKPFHYDRPWQRATGKEANECLPCNCNLHARRCRFNMELYKLSGRKSGGVCMNCRHNTAGRHCHYCKEGFYRDMGKAITHRRACKACDCHPVGAAG-KTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPVAPCIKIPVIKPTAVISTTEEPADCDSYCKPLKGNLKINMKKYCKKDYAVQVSVLDMETVGDWAKFSVSLMSVYK-------------------SRGDPLKRGDHMLWVHMKDLACKCPRIHMGKRFLILGSTEGTANPERPGLQADKNSLVIQWRDIWTRRLRKFQRKEKKGKCSK 599
BLAST of netrin 1 vs. Ensembl Zebrafish
Match: ntn1b (netrin 1b [Source:ZFIN;Acc:ZDB-GENE-990415-168]) HSP 1 Score: 335.109 bits (858), Expect = 2.249e-105 Identity = 206/642 (32.09%), Postives = 325/642 (50.62%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICV-------DHINCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMA---------QAPENRCQHCNNKRKRIR--FKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLC 2029
CY E G RC+P F N ++STCG + R CV +C+ C K TH YLTD + +N+T W S+ +V + NV +T S GK FE+ Y+SL P+S+ I KS+D G +W + ++S C + + N S + IT+ +Q CT DS + ++AF+T + +H D W++AT+I++T FNR++ + + + + S F+A++D+ +GGRC+C GHA +C+K+ N C+C+HNTAG +C+ C+ D+ W AT A EC C+CNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +GY+RD SK +SH C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ KIP+A P + HC + +++ KKYC+KD + V + ++ GE +F + + VY+ + +Q +W++ D+ CKCP ++ G YL++ +S ++ D+ S+ +PW+ W RR++KF ++ +G C
Sbjct: 40 CYDENGNPRRCIPDFVNSAFGKDV-RVSSTCG-SPPSRYCVVTEKGEERSRDCNICDATDPKKTHPPAYLTDLNNPHNLTCWQSENYVQYPQNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIFKSMDYGKTWVPFQFYSTQCKKMY-------NKPSKAAITKQN-EQEAICT---DSHTDMQPLTGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVT-------------------------FNRLH-------------TFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASKCVKDREGNLV-CECKHNTAGPECDRCKPFHYDRPWQRATAREANECVACHCNLHARRCRFNMELYKLSGRRSGGVCLNCRHNTAGRHCHYCKEGYYRDMSKAISHRRACKACDCHPVGAA-GKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCI------KIPVAAPTSSYSSPDGPTDCDSHCKAPKGKMKVTMKKYCKKDFAVQVHVLKGDKAGEWWKFTINIISVYKQGGHRIRR-------------GDQL------LWVRAKDVACKCPKIKPGRKYLLMGSDEEEDSRGQSGVVADRGSLLIPWKDLWARRLRKFQQRDKRGKC 602
BLAST of netrin 1 vs. Ensembl Zebrafish
Match: ntn1b (netrin 1b [Source:ZFIN;Acc:ZDB-GENE-990415-168]) HSP 1 Score: 335.109 bits (858), Expect = 2.249e-105 Identity = 206/642 (32.09%), Postives = 325/642 (50.62%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICV-------DHINCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMA---------QAPENRCQHCNNKRKRIR--FKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLC 2029
CY E G RC+P F N ++STCG + R CV +C+ C K TH YLTD + +N+T W S+ +V + NV +T S GK FE+ Y+SL P+S+ I KS+D G +W + ++S C + + N S + IT+ +Q CT DS + ++AF+T + +H D W++AT+I++T FNR++ + + + + S F+A++D+ +GGRC+C GHA +C+K+ N C+C+HNTAG +C+ C+ D+ W AT A EC C+CNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +GY+RD SK +SH C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ KIP+A P + HC + +++ KKYC+KD + V + ++ GE +F + + VY+ + +Q +W++ D+ CKCP ++ G YL++ +S ++ D+ S+ +PW+ W RR++KF ++ +G C
Sbjct: 40 CYDENGNPRRCIPDFVNSAFGKDV-RVSSTCG-SPPSRYCVVTEKGEERSRDCNICDATDPKKTHPPAYLTDLNNPHNLTCWQSENYVQYPQNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIFKSMDYGKTWVPFQFYSTQCKKMY-------NKPSKAAITKQN-EQEAICT---DSHTDMQPLTGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVT-------------------------FNRLH-------------TFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASKCVKDREGNLV-CECKHNTAGPECDRCKPFHYDRPWQRATAREANECVACHCNLHARRCRFNMELYKLSGRRSGGVCLNCRHNTAGRHCHYCKEGYYRDMSKAISHRRACKACDCHPVGAA-GKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCI------KIPVAAPTSSYSSPDGPTDCDSHCKAPKGKMKVTMKKYCKKDFAVQVHVLKGDKAGEWWKFTINIISVYKQGGHRIRR-------------GDQL------LWVRAKDVACKCPKIKPGRKYLLMGSDEEEDSRGQSGVVADRGSLLIPWKDLWARRLRKFQQRDKRGKC 602
BLAST of netrin 1 vs. Ensembl Xenopus
Match: rps16 (ribosomal protein S16 [Source:Xenbase;Acc:XB-GENE-5786511]) HSP 1 Score: 323.553 bits (828), Expect = 5.848e-101 Identity = 208/630 (33.02%), Postives = 312/630 (49.52%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASD-FVNHGD-NVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNS-----WMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPM---AQAPENRCQHCNNKRK--RIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
CY E G RCVP F N N + +STCG R+ H + K ++ +YLTD + N+T W S+ F H NV++T S GK FE+ Y+SL P+S+ I KS+D G SW + Y+S C + +G +P S + +T+ ++L CT D + + ++AF+T + A D NS W++AT+IR+ F PHL + K + P ++++ + +GGRC+C GHA RC K+ + CDC+HNT G +C+ C+ D+ W AT A EC C CNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +G++RD S+ ++ C C CHP+G+ C+Q QC CK GV GL+CNRC KG+QQ++SPI PC+ + + P + P + Q+C + +I KKYC+KD V+ V + E G A+F + + VY+ + + F +WI DL CKCP + L YLV+ ER L D+ S+ + W+ W RR++K +E +G C K
Sbjct: 41 CYDEHGLPRRCVPEFVNAAFNK-EVQASSTCG-----RLATRHCDTTDPRK-SYPASYLTDLNTAGNMTCWRSETFPRHSSQNVSLTLSLGKKFEVTYVSLQFCSP-RPESVAIFKSMDYGKSWVPYQYYSSQCRKVYG-KP------SRASVTKQNEQEAL-CT---DGQTELYPLTGGLIAFSTLDGR--PSAQDLDNSPVLQDWLTATDIRV----------------FFSRPHL------------------LGNKESEASDEGPGF-----YYSVGEFQVGGRCKCNGHASRCGKD-KEARLICDCKHNTEGPECDRCKPFHYDRPWQRATAREANECMACTCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDVSRAITDRRACKACDCHPVGAAG-KTCNQTTGQCPCKDGVTGLTCNRCAKGYQQSRSPIAPCIKIPAVVPTPALTSTEEPADCEQYCKPPKGNYKINLKKYCKKDFVVQVNVLEMEMVGSWAKFTVNILSVYKCRDDRIKR-------------GDNF------LWIHIKDLSCKCPKILLSRRYLVMGSSEGPS--ERPGLTADKNSMVIQWRDAWTRRLRKLQRREKKGKCLK 587
BLAST of netrin 1 vs. Ensembl Xenopus
Match: rps16 (ribosomal protein S16 [Source:Xenbase;Acc:XB-GENE-5786511]) HSP 1 Score: 305.064 bits (780), Expect = 3.388e-94 Identity = 200/625 (32.00%), Postives = 300/625 (48.00%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASD-FVNHGD-NVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNS-----WMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
CY E G RCVP F N N + +STCG R+ H + K ++ +YLTD + N+T W S+ F H NV++T S GK FE+ Y+SL P+S+ I KS+D G SW + Y+S C + +G +P S + +T+ ++L CT D + + ++AF+T + A D NS W++AT+IR+ F PHL + K + P ++++ + +GGRC+C GHA RC K+ + CDC+HNT G +C+ C+ D+ W AT A EC C CNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +G++RD S+ ++ C C CHP+G+ C+Q QC CK GV GL+CNRC KG+QQ++SPI PC+ + + P + E + AV+ V + E G A+F + + VY+ + + F +WI DL CKCP + L YLV+ ER L D+ S+ + W+ W RR++K +E +G C K
Sbjct: 41 CYDEHGLPRRCVPEFVNAAFNK-EVQASSTCG-----RLATRHCDTTDPRK-SYPASYLTDLNTAGNMTCWRSETFPRHSSQNVSLTLSLGKKFEVTYVSLQFCSP-RPESVAIFKSMDYGKSWVPYQYYSSQCRKVYG-KP------SRASVTKQNEQEAL-CT---DGQTELYPLTGGLIAFSTLDGR--PSAQDLDNSPVLQDWLTATDIRV----------------FFSRPHL------------------LGNKESEASDEGPGF-----YYSVGEFQVGGRCKCNGHASRCGKD-KEARLICDCKHNTEGPECDRCKPFHYDRPWQRATAREANECMACTCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDVSRAITDRRACKACDCHPVGAAG-KTCNQTTGQCPCKDGVTGLTCNRCAKGYQQSRSPIAPCIKIPAVVPTPALTSTE--------------------EPAVVQVNVLEMEMVGSWAKFTVNILSVYKCRDDRIKR-------------GDNF------LWIHIKDLSCKCPKILLSRRYLVMGSSEGPS--ERPGLTADKNSMVIQWRDAWTRRLRKLQRREKKGKCLK 562
BLAST of netrin 1 vs. Ensembl Xenopus
Match: ntn1 (netrin 1 [Source:NCBI gene;Acc:779839]) HSP 1 Score: 303.138 bits (775), Expect = 3.739e-93 Identity = 208/678 (30.68%), Postives = 327/678 (48.23%), Query Frame = 2
Query: 104 GICPFVIFAILIQCQA-------QHWLHGGS-NSLCY-SEGKAFRCVPPFTNIIENMIPDEITS-TCGQNKKQRIC--VDHIN-----CHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFT--TNNPNFFS-HASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPE-------NRCQ-HCNNKRKRIR--FKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCE 2032
G PF I ++L + HGG+ + CY ++ + C+P F N+ + + ITS TCG+ Q++C +D N C C ++++ +Y+TD + T W S+ + NV +T G+ FE+ Y SL PDS+ I KS+D G +W + ++S C R + + + S I++ + TCT D + G + ++AF P+ S W++AT+IR+ FNR++ + + + RK + F+ +++I +GGRC+C GHA RC C+CQHNT G +C+ C+ + D+ W AT ++A EC C CNLHS +C FN +LY +S ++SGGVC++C HNT GR+CH C +GY RD SKP++ C C+CHPIG++ +C+Q QC CKTGV G +CNRC G+QQ++S PCV + + + E CQ +C R+ +KYC+KD VL + S E+ GE +F V VYR P+W+ + DL C C +Q+G +YL+I + + + L+LD+ S+ALPW+ W ++++F +E +G C
Sbjct: 7 GFIPFYIISLLCPVPRGQENNSNKASTHGGTPDGPCYDAKQRPKYCIPDFINVA--LGQEVITSNTCGRTP-QKLCYFLDSTNVTSRRCQLCDQKRPENSYPPSYMTD---TDAQTCWKSESGITFPQNVTLTLPLGRRFELVYASLRFCSP-RPDSMAIYKSMDYGKTWIPFQFYSTHCRRVY-------DQATTSAISKP-MQHEATCT---DFQTGVKPLTKGLVAFMPLAGRPSARRFEYSPVLQDWVTATDIRVV-------------------------FNRLH----HGKKMGIRRK--------------TAFYGVSEIQVGGRCKCNGHASRCTAG--KEGLMCECQHNTIGPECDACKPFYYDRPWQRATPSNAHECVACECNLHSHRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKQGYKRDLSKPITSRKACKPCQCHPIGAVN-AMCNQTTGQCQCKTGVTGPTCNRCAPGYQQSRSAHMPCVRIQEEMTTTASYPQEWKAGTNGTECQSYCKPSHSRVHMNLRKYCKKDYVLRAQLLSMEKSGEWWQFTATVLTVYRQRRVPIRR-------------------GDQPLWVPDHDLACDCLRVQVGKSYLIIGNDE--ESPDPARLILDKNSLALPWRDVWGHKLRRFQQQERRGKCR 599
BLAST of netrin 1 vs. Ensembl Xenopus
Match: utp4 (UTP4 small subunit processome component [Source:Xenbase;Acc:XB-GENE-977755]) HSP 1 Score: 188.348 bits (477), Expect = 4.374e-49 Identity = 140/467 (29.98%), Postives = 207/467 (44.33%), Query Frame = 2
Query: 185 CYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDH------INCHSCMKDT----HSINYLTDQHLNNNITFWASDFVNHG----DNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFS-HASDQYNSWMSATNIRITLRKSETNFLNSHLDG-FTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQ-TKSPITPCV 1534
CY EG RC+P F N N T+TCG + CV +CH C H YLTD + IT+W S + G +N+T GK F+I Y+ L P+S + K W + Y+S C +++ ++ I +Q CT S + L + P+ ++ S W++AT+IR+TL N LN+ D F+ P LK Y+ +A++D ++GGRC+C GHA C++N + C+C+HNT G DCE C + D+ W +T +S EC CNCN S++C F+ +LY + GG C C NTDG C +C + Y+R ++ C C+C+P+GS+ CD +C+CK GV G C+RCQ GF T++ PC
Sbjct: 4 CYEEGSPQRCMPEFVNAAFNATV-VATNTCG-TPPEEYCVQTGVTGVTKSCHICDSGQFHLQHGAEYLTDYNNQAEITWWQSQTMLAGIQYPSTINLTLHLGKAFDITYVRLKFH-TSRPESFALYKRTHEDGPWIPYQYYSGSCEKTY-------QKFNRGFIRTGEDEQQALCTD-EFSDISPLTGGNVAFSTLEGRPSAYNFDNSPVLQEWVTATDIRVTL-----NRLNTFGDEVFSDPKVLKSYY-----------------------------------YAISDFAVGGRCKCNGHASECVRNEFEKIV-CNCKHNTFGSDCEKCLPFYNDRPWRRSTADSPNECLPCNCNGRSQECYFDPELYRSTGH--GGHCTGCADNTDGPNCERCRENYYRQ-----DNNEPCHACQCNPVGSLSTQ-CDNY-GRCSCKPGVMGEKCDRCQPGFHSLTEAGCRPCA 409 HSP 2 Score: 80.1073 bits (196), Expect = 9.806e-15 Identity = 63/197 (31.98%), Postives = 88/197 (44.67%), Query Frame = 2
Query: 1025 CQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCR-CHPIGSIYPDV---CDQRNSQC-TCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCN 1600
C C GH+ C +P CDCQHNTAG CE C EG+ + +T SA++C+ C C S C + V VC +C T G+ C C+ GY D PL + CR C +I P+ CD+ +C C GG C+RC+ GF + P+AQ P+ +C+ C+
Sbjct: 687 CTCNGHSDTC--DPESGV--CDCQHNTAGPHCERCSEGY----YGDSTTGSASDCQPCPCPGGS-SCAVVPRTKEV-------VCTNCPLGTTGKRCELCDDGYFGD---PLGENGAPRPCRICECSNNIDPNAVGNCDRLTGECLKCIYNTGGFYCDRCRDGF----------------YGNPLAQNPDLKCRACS 848 HSP 3 Score: 54.299 bits (129), Expect = 9.179e-7 Identity = 44/140 (31.43%), Postives = 59/140 (42.14%), Query Frame = 2
Query: 1085 CDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCT-CKTGVGGLSCNRCQKGF 1501
C C G+ CE C G+ R + + C C CN HS C+ GVC DC+HNT G +C +C++GY+ D + + C C C P GS V + CT C G G C C G+
Sbjct: 653 CSCPTGYIGQFCERCAPGY---RRENPSQGPYSPCVLCTCNGHSDTCD-----------PESGVC-DCQHNTAGPHCERCSEGYYGDSTTGSASD--CQPCPC-PGGSSCAVVPRTKEVVCTNCPLGTTGKRCELCDDGY 774
BLAST of netrin 1 vs. Ensembl Xenopus
Match: utp4 (UTP4 small subunit processome component [Source:Xenbase;Acc:XB-GENE-977755]) HSP 1 Score: 188.348 bits (477), Expect = 4.543e-49 Identity = 140/467 (29.98%), Postives = 207/467 (44.33%), Query Frame = 2
Query: 185 CYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDH------INCHSCMKDT----HSINYLTDQHLNNNITFWASDFVNHG----DNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFS-HASDQYNSWMSATNIRITLRKSETNFLNSHLDG-FTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQ-TKSPITPCV 1534
CY EG RC+P F N N T+TCG + CV +CH C H YLTD + IT+W S + G +N+T GK F+I Y+ L P+S + K W + Y+S C +++ ++ I +Q CT S + L + P+ ++ S W++AT+IR+TL N LN+ D F+ P LK Y+ +A++D ++GGRC+C GHA C++N + C+C+HNT G DCE C + D+ W +T +S EC CNCN S++C F+ +LY + GG C C NTDG C +C + Y+R ++ C C+C+P+GS+ CD +C+CK GV G C+RCQ GF T++ PC
Sbjct: 28 CYEEGSPQRCMPEFVNAAFNATV-VATNTCG-TPPEEYCVQTGVTGVTKSCHICDSGQFHLQHGAEYLTDYNNQAEITWWQSQTMLAGIQYPSTINLTLHLGKAFDITYVRLKFH-TSRPESFALYKRTHEDGPWIPYQYYSGSCEKTY-------QKFNRGFIRTGEDEQQALCTD-EFSDISPLTGGNVAFSTLEGRPSAYNFDNSPVLQEWVTATDIRVTL-----NRLNTFGDEVFSDPKVLKSYY-----------------------------------YAISDFAVGGRCKCNGHASECVRNEFEKIV-CNCKHNTFGSDCEKCLPFYNDRPWRRSTADSPNECLPCNCNGRSQECYFDPELYRSTGH--GGHCTGCADNTDGPNCERCRENYYRQ-----DNNEPCHACQCNPVGSLSTQ-CDNY-GRCSCKPGVMGEKCDRCQPGFHSLTEAGCRPCA 433 HSP 2 Score: 76.2554 bits (186), Expect = 1.316e-13 Identity = 63/197 (31.98%), Postives = 86/197 (43.65%), Query Frame = 2
Query: 1025 CQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCR-CHPIGSIYPDV---CDQRNSQC-TCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCN 1600
C C GH+ C +P CDCQHNTAG CE C EG+ + +T SA++C+ C C S C + V VC +C T G+ C C+ GY D PL + CR C +I P+ CD+ +C C GG C+RC+ GF + P+AQ P+ + CN
Sbjct: 711 CTCNGHSDTC--DPESGV--CDCQHNTAGPHCERCSEGY----YGDSTTGSASDCQPCPCPGGS-SCAVVPRTKEV-------VCTNCPLGTTGKRCELCDDGYFGD---PLGENGAPRPCRICECSNNIDPNAVGNCDRLTGECLKCIYNTGGFYCDRCRDGF----------------YGNPLAQNPDLKSCSCN 872 HSP 3 Score: 54.299 bits (129), Expect = 9.261e-7 Identity = 44/140 (31.43%), Postives = 59/140 (42.14%), Query Frame = 2
Query: 1085 CDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCT-CKTGVGGLSCNRCQKGF 1501
C C G+ CE C G+ R + + C C CN HS C+ GVC DC+HNT G +C +C++GY+ D + + C C C P GS V + CT C G G C C G+
Sbjct: 677 CSCPTGYIGQFCERCAPGY---RRENPSQGPYSPCVLCTCNGHSDTCD-----------PESGVC-DCQHNTAGPHCERCSEGYYGDSTTGSASD--CQPCPC-PGGSSCAVVPRTKEVVCTNCPLGTTGKRCELCDDGY 798
BLAST of netrin 1 vs. Ensembl Mouse
Match: Ntn1 (netrin 1 [Source:MGI Symbol;Acc:MGI:105088]) HSP 1 Score: 335.495 bits (859), Expect = 2.031e-105 Identity = 202/638 (31.66%), Postives = 327/638 (51.25%), Query Frame = 2
Query: 197 GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHI------NCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMA---------QAPENRCQHCNNKRKRIR--FKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
G RC+P F N ++STCG+ + V +CH C K H +LTD + +N+T W S+ ++ NV +T S GK FE+ Y+SL P+S+ I KS+D G +W + ++S C + + N + IT+ +Q CT DS S ++AF+T + +H D W++AT+IR+ F+R++ + + + + S ++A++D+ +GGRC+C GHA RC+++ D++ CDC+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +G++RD KP++H C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ KIP+A + PE+ +C + +++ KKYCRKD + + + ++ G+ +F + + VY+ TS+ G S +WI+ D+ CKCP ++ YL++ +A ++S ++ D+ S+ + W+ TW RR++KF +E +G C+K
Sbjct: 46 GHPRRCIPDFVNAAFGKDV-RVSSTCGRPPARYCVVSERGEERLRSCHLCNSSDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIYKSMDYGRTWVPFQFYSTQCRKMY-------NRPHRAPITKQN-EQEAVCT---DSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVA-------------------------FSRLH-------------TFGDENEDDSELARDSYYYAVSDLQVGGRCKCNGHAARCVRD-RDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDMGKPITHRKACKACDCHPVGAAG-KTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCI------KIPVAPPTTAASSVEEPEDCDSYCKASKGKLKMNMKKYCRKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRR----------GDQS---------LWIRSRDIACKCPKIKPLKKYLLL--GNAEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKK 603
BLAST of netrin 1 vs. Ensembl Mouse
Match: Ntn1 (netrin 1 [Source:MGI Symbol;Acc:MGI:105088]) HSP 1 Score: 335.495 bits (859), Expect = 2.031e-105 Identity = 202/638 (31.66%), Postives = 327/638 (51.25%), Query Frame = 2
Query: 197 GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHI------NCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMA---------QAPENRCQHCNNKRKRIR--FKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
G RC+P F N ++STCG+ + V +CH C K H +LTD + +N+T W S+ ++ NV +T S GK FE+ Y+SL P+S+ I KS+D G +W + ++S C + + N + IT+ +Q CT DS S ++AF+T + +H D W++AT+IR+ F+R++ + + + + S ++A++D+ +GGRC+C GHA RC+++ D++ CDC+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +G++RD KP++H C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ KIP+A + PE+ +C + +++ KKYCRKD + + + ++ G+ +F + + VY+ TS+ G S +WI+ D+ CKCP ++ YL++ +A ++S ++ D+ S+ + W+ TW RR++KF +E +G C+K
Sbjct: 46 GHPRRCIPDFVNAAFGKDV-RVSSTCGRPPARYCVVSERGEERLRSCHLCNSSDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIYKSMDYGRTWVPFQFYSTQCRKMY-------NRPHRAPITKQN-EQEAVCT---DSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVA-------------------------FSRLH-------------TFGDENEDDSELARDSYYYAVSDLQVGGRCKCNGHAARCVRD-RDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDMGKPITHRKACKACDCHPVGAAG-KTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCI------KIPVAPPTTAASSVEEPEDCDSYCKASKGKLKMNMKKYCRKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRR----------GDQS---------LWIRSRDIACKCPKIKPLKKYLLL--GNAEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKK 603
BLAST of netrin 1 vs. Ensembl Mouse
Match: Ntn3 (netrin 3 [Source:MGI Symbol;Acc:MGI:1341188]) HSP 1 Score: 276.559 bits (706), Expect = 1.869e-83 Identity = 188/621 (30.27%), Postives = 275/621 (44.28%), Query Frame = 2
Query: 173 SNSLCYSEGKAFR-CVPPFTNIIENMIPDEI--TSTCGQNKKQRICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASDFVNHGD-NVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNN--PNFFS-HASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYH--CDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCV--DVIDLHKIPMAQAPENRCQHCNNKRK--RIRFKKYCRKDAVLLVTMQSR-EQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSE----LLLDQKSVALPWQKTW 1981
S CY E + R C+P ++ + E+ +STCG++ R+C S + HS + LT + W SD + NV +T GK FE+ ++SL P S+ + KS D+G SW +FS C +G P + S C P ++ + +LAF+ + P S W++AT+IRI L + ++ G T P YS ++ ++ +GGRC+C GHA RCL +D H CDCQH T G DC C+ + D+ W AT A C C+CN H+++C FN +LY +S ++SGGVC++C HNT GR+CH C +G++RD + LS C C CHP+G+ C+Q QC CK GV GL+CNRC GFQQ++SP+ PCV V + P++ HC R RI KK+CRKD + V + +R E G RF + V V+R + + +W+ D C CP L G YL++ + L + S+ LPW+ W
Sbjct: 26 SADPCYDEAREPRSCIP---GLVNAALGREVLASSTCGRSAN-RVCDS-----SDPQRAHSADLLTSAPGTASPLCWRSDLLQQAPFNVTLTVPLGKAFELVFVSLRFC-SAPPTSVALLKSQDHGRSWVPLGFFSSSCTLDYGRLPAPADG-------PSGPGPEALCFPAPQAQ----PDGGGLLAFSVQDGSPQGLDLDNSPVLQDWVTATDIRIVLTRPAIQG-DTRDGGVTVP---------------YS-------------------------YSATELQVGGRCKCNGHASRCL---LDTHGHLVCDCQHGTEGPDCSRCKPFYCDRPWQRATGQEAHACLACSCNGHARRCRFNMELYRLSGRRSGGVCLNCRHNTAGRHCHYCREGFYRDPGRVLSDRRACRACDCHPVGAAG-KTCNQTTGQCPCKDGVTGLTCNRCAPGFQQSRSPVAPCVKTPVPGPTEESSPVEPQDCESHCRPARGSYRISLKKFCRKDYAVQVAVGARGEARGSWTRFPVAVLAVFRSGEERAR-------------------RGSSALWVPTLDAACGCPRLLPGRRYLLLGGGPGAAAGSTAGRGQGLSAARGSLVLPWRDAW 561
BLAST of netrin 1 vs. Ensembl Mouse
Match: Ntn5 (netrin 5 [Source:MGI Symbol;Acc:MGI:2685330]) HSP 1 Score: 208.764 bits (530), Expect = 2.878e-60 Identity = 120/364 (32.97%), Postives = 179/364 (49.18%), Query Frame = 2
Query: 974 LKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVID----LHKIPMAQAPENRCQ-HCNNKRKRIR--FKKYCRKDAVLLVTMQ-SREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVI---LKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCE 2032
++ L + GRCQC+GHA RC C C+H+T G CE CR D W AT C C+CN H+++C FN +L+ +S +SGGVC C H+T GR+CH C G+ RD S+P++ H C C+CHPIG+ +C+Q + QC+CK GV GL+CNRC G+QQ++SP PC + + P+A + +CQ +CN + ++YC++D VL + S Q E E +R+ G W+ DL C C L+ G YL++ + H + + L+L++ +ALPW+ W R +++ KE G C
Sbjct: 24 VEFGGKAGLVTTGVRGRCQCHGHAARCATR--AQPPRCRCRHHTTGPGCESCRPSHRDWPWRPATPQHPHPCLPCSCNQHARRCRFNSELFRLSGGRSGGVCERCRHHTAGRHCHYCQPGFWRDPSQPITSHKACRACQCHPIGATG-GMCNQTSGQCSCKLGVTGLTCNRCGPGYQQSRSPRMPCQRIPEATTTPATTPVASRSDPQCQGYCNVSVSSVHMSLQRYCQQDYVLHAQVSASSSQPSEAVGPEW-----WRLAVHVLAVFKQRAWPVRRGGQE---------AWVPRADLICGCLRLRPGADYLLLGRAAQTHDDDNYDPARLILNRHGLALPWRPRWARPLRRLQQKERGGACR 370
BLAST of netrin 1 vs. Ensembl Mouse
Match: Lamc1 (laminin, gamma 1 [Source:MGI Symbol;Acc:MGI:99914]) HSP 1 Score: 187.193 bits (474), Expect = 9.718e-49 Identity = 139/461 (30.15%), Postives = 206/461 (44.69%), Query Frame = 2
Query: 197 GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDH------INCHSCMKDT----HSINYLTDQHLNNNITFWASDF----VNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFS-HASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQ-TKSPITPC 1531
G+ RC+P F N N+ T+TCG + CV +CH C H +LTD + + T+W S V + +++N+T GK F+I Y+ L P+S I K W + Y+S C ++ F I +Q CT S + L + P+ ++ S W++AT+IR+TL + L + + ++ N P VLK S ++A++D ++GGRC+C GHA C+KN D C+C+HNT G DCE C F D+ W AT SA+EC C+CN S++C F+ +LY + GG C +C NTDG C +C + + R L + C C C P+GS+ CD +C+CK GV G C+RCQ GF T++ PC
Sbjct: 43 GRPQRCMPEFVNAAFNVTV-VATNTCG-TPPEEYCVQTGVTGVTKSCHLCDAGQQHLQHGAAFLTDYNNQADTTWWQSQTMLAGVQYPNSINLTLHLGKAFDITYVRLKFH-TSRPESFAIYKRTREDGPWIPYQYYSGSCENTYSKANRGF-------IRTGGDEQQALCTD-EFSDISPLTGGNVAFSTLEGRPSAYNFDNSPVLQEWVTATDIRVTLNR------------------LNTFGDEVF--------------------NDPKVLK-SYYYAISDFAVGGRCKCNGHASECVKNEFDK-LMCNCKHNTYGVDCEKCLPFFNDRPWRRATAESASECLPCDCNGRSQECYFDPELYRSTGH--GGHCTNCRDNTDGAKCERCRENFFR-----LGNTEACSPCHCSPVGSLSTQ-CDSY-GRCSCKPGVMGDKCDRCQPGFHSLTEAGCRPC 443 HSP 2 Score: 65.4698 bits (158), Expect = 2.488e-10 Identity = 55/195 (28.21%), Postives = 85/195 (43.59%), Query Frame = 2
Query: 1025 CQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDV---CDQRNSQC-TCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHC 1597
C C GH+ C +P T CDC+ NTAG CE C +G+ + +T+ ++++C+ C C S C K V VC C T G+ C C+ GY D +C C+C+ +I P+ C++ +C C G C+RC++GF P+A P ++C+ C
Sbjct: 722 CTCNGHSETC--DP--ETGVCDCRDNTAGPHCEKCSDGY----YGDSTLGTSSDCQPCPCPGGS-SCAIVPKTKEV-------VCTHCPTGTAGKRCELCDDGYFGDPLGSNGPVRLCRPCQCN--DNIDPNAVGNCNRLTGECLKCIYNTAGFYCDRCKEGF----------------FGNPLAPNPADKCKAC 882 HSP 3 Score: 63.1586 bits (152), Expect = 1.233e-9 Identity = 51/154 (33.12%), Postives = 65/154 (42.21%), Query Frame = 2
Query: 1046 GRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSG-----GVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQ 1492
G CLK C +NTAG C+ C+EGF + N A +CK C CN Y +QS G C C + GR C C+ GY+ L C +C CH +GS CD R QC C+ G+ G C RC+
Sbjct: 845 GECLK----------CIYNTAGFYCDRCKEGFFGNPLAP---NPADKCKACACNP-----------YGTVQQQSSCNPVTGQC-QCLPHVSGRDCGTCDPGYYN-----LQSGQGCERCDCHALGSTNGQ-CDIRTGQCECQPGITGQHCERCE 967 HSP 4 Score: 57.3806 bits (137), Expect = 8.045e-8 Identity = 45/140 (32.14%), Postives = 59/140 (42.14%), Query Frame = 2
Query: 1085 CDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCT-CKTGVGGLSCNRCQKGF 1501
C C G+ CE C G+ R ++ + C C CN HS+ C+ GVC DC NT G +C +C+ GY+ D + L C C C P GS V + CT C TG G C C G+
Sbjct: 688 CTCPVGYGGQFCETCLPGY---RRETPSLGPYSPCVLCTCNGHSETCD-----------PETGVC-DCRDNTAGPHCEKCSDGYYGDST--LGTSSDCQPCPC-PGGSSCAIVPKTKEVVCTHCPTGTAGKRCELCDDGY 809
BLAST of netrin 1 vs. UniProt/SwissProt
Match: sp|Q90922|NET1_CHICK (Netrin-1 OS=Gallus gallus OX=9031 GN=NTN1 PE=1 SV=1) HSP 1 Score: 338.191 bits (866), Expect = 1.095e-105 Identity = 210/670 (31.34%), Postives = 336/670 (50.15%), Query Frame = 2
Query: 89 QSLRNGICPFVIFAILIQCQAQHWLHGGSNSLCYSE-GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICV------DHI-NCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDV---IDLHKIPMAQAPENRCQHCNNKRKRIR--FKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
Q LR G +FA+ CY E G RC+P F N +++STCG+ R CV + + +CH C K H ++LTD + +N+T W SD +V + NV +T S GK FE+ Y+SL P+S+ I KS+D G +W + ++S C + + N S + IT+ +Q CT DS S ++AF+T + +H D W++AT+I++T F+R++ + + + + S F+A++D+ +GGRC+C GHA RC+++ DN CDC+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +G++RD SKP+SH C +C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ + + P + +C + +++ KKYC+KD + + + E++ + +F + + VY+ SN+ +W+ D+ CKCP ++ YL++ ++S ++ D+ S+ + W+ TW RR++KF +E +G C K
Sbjct: 21 QPLRGGYPGLNMFAV----------QTAQPDPCYDEHGLPRRCIPDFVNSAFGK-EVKVSSTCGK-PPSRYCVVTEKGEEQVRSCHLCNASDPKRAHPPSFLTDLNNPHNLTCWQSDSYVQYPHNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIYKSMDYGKTWVPFQFYSTQCRKMY-------NKPSRAAITKQN-EQEAICT---DSHTDVRPLSGGLIAFSTLDGRPTAHDFDNSPVLQDWVTATDIKVT-------------------------FSRLH-------------TFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASRCVRDRDDNLV-CDCKHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDLSKPISHRKACKECDCHPVGAA-GQTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPAAPPPTAASSTEEPADCDSYCKASKGKLKINMKKYCKKDYAVQIHILKAEKNADWWKFTVNIISVYKQG-------------------SNRLRRGDQTLWVHAKDIACKCPKVKPMKKYLLLGSTE--DSPDQSGIIADKSSLVIQWRDTWARRLRKFQQREKKGKCRK 605
BLAST of netrin 1 vs. UniProt/SwissProt
Match: sp|O95631|NET1_HUMAN (Netrin-1 OS=Homo sapiens OX=9606 GN=NTN1 PE=1 SV=2) HSP 1 Score: 336.265 bits (861), Expect = 5.957e-105 Identity = 203/638 (31.82%), Postives = 327/638 (51.25%), Query Frame = 2
Query: 197 GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHI------NCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMA---------QAPENRCQHCNNKRKRIR--FKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
G RC+P F N ++STCG+ + V +CH C K H +LTD + +N+T W S+ ++ NV +T S GK FE+ Y+SL P+S+ I KS+D G +W + ++S C + + N + IT+ +Q CT DS S ++AF+T + +H D W++AT+IR+ F+R++ + + + + S F+A++D+ +GGRC+C GHA RC+++ D++ CDC+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +GY+RD KP++H C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ KIP+A + PE+ +C + +++ KKYC+KD + + + ++ G+ +F + + VY+ TS+ G S +WI+ D+ CKCP ++ YL++ +A ++S ++ D+ S+ + W+ TW RR++KF +E +G C+K
Sbjct: 46 GHPRRCIPDFVNAAFGKDV-RVSSTCGRPPARYCVVSERGEERLRSCHLCNASDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIYKSMDYGRTWVPFQFYSTQCRKMY-------NRPHRAPITKQN-EQEAVCT---DSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVA-------------------------FSRLH-------------TFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHAARCVRD-RDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMGKPITHRKACKACDCHPVGAAG-KTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCI------KIPVAPPTTAASSVEEPEDCDSYCKASKGKLKINMKKYCKKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRR----------GDQS---------LWIRSRDIACKCPKIKPLKKYLLL--GNAEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKK 603
BLAST of netrin 1 vs. UniProt/SwissProt
Match: sp|O09118|NET1_MOUSE (Netrin-1 OS=Mus musculus OX=10090 GN=Ntn1 PE=1 SV=3) HSP 1 Score: 335.495 bits (859), Expect = 1.420e-104 Identity = 202/638 (31.66%), Postives = 327/638 (51.25%), Query Frame = 2
Query: 197 GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHI------NCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMA---------QAPENRCQHCNNKRKRIR--FKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
G RC+P F N ++STCG+ + V +CH C K H +LTD + +N+T W S+ ++ NV +T S GK FE+ Y+SL P+S+ I KS+D G +W + ++S C + + N + IT+ +Q CT DS S ++AF+T + +H D W++AT+IR+ F+R++ + + + + S ++A++D+ +GGRC+C GHA RC+++ D++ CDC+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +G++RD KP++H C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ KIP+A + PE+ +C + +++ KKYCRKD + + + ++ G+ +F + + VY+ TS+ G S +WI+ D+ CKCP ++ YL++ +A ++S ++ D+ S+ + W+ TW RR++KF +E +G C+K
Sbjct: 46 GHPRRCIPDFVNAAFGKDV-RVSSTCGRPPARYCVVSERGEERLRSCHLCNSSDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIYKSMDYGRTWVPFQFYSTQCRKMY-------NRPHRAPITKQN-EQEAVCT---DSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVA-------------------------FSRLH-------------TFGDENEDDSELARDSYYYAVSDLQVGGRCKCNGHAARCVRD-RDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDMGKPITHRKACKACDCHPVGAAG-KTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCI------KIPVAPPTTAASSVEEPEDCDSYCKASKGKLKMNMKKYCRKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRR----------GDQS---------LWIRSRDIACKCPKIKPLKKYLLL--GNAEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKK 603
BLAST of netrin 1 vs. UniProt/SwissProt
Match: sp|Q924Z9|NET1_RAT (Netrin-1 OS=Rattus norvegicus OX=10116 GN=Ntn1 PE=1 SV=1) HSP 1 Score: 328.561 bits (841), Expect = 4.801e-102 Identity = 200/638 (31.35%), Postives = 324/638 (50.78%), Query Frame = 2
Query: 197 GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHI------NCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMA---------QAPENRCQHCNNKRKRIR--FKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
G RC+P F N ++STCG+ + V +CH C K H +LTD + +N+T W S+ ++ NV +T S GK FE+ Y+SL P+S+ I KS+D G +W + ++S C + + N + IT+ +Q CT DS S ++AF+T + +H D W++AT+IR+ F+R++ + + + + S ++A++D+ +GGRC+C GHA RC+++ D++ CDC+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +G++RD KP++H C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ KIP+A + PE+ +C + +++ KKYCRKD + + + ++ G+ +F + + VY+ TS+ G S +WI+ D+ KCP ++ YL++ +A ++S ++ D+ + + W+ TW RR +KF +E +G C+K
Sbjct: 46 GHPRRCIPDFVNAAFGKDV-RVSSTCGRPPARYCVVSERGEERLRSCHLCNSSDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIYKSMDYGRTWVPFQFYSTQCRKMY-------NRPHRAPITKQN-EQEAVCT---DSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVA-------------------------FSRLH-------------TFGDENEDDSELARDSYYYAVSDLQVGGRCKCNGHAARCVRD-RDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDMGKPITHRKACKACDCHPVGAAG-KTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCI------KIPVAPPTTAASSMEEPEDCDSYCKASKGKLKMNMKKYCRKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRR----------GDQS---------LWIRSRDIAXKCPKIKPLKKYLLL--GNAEDSPDQSGIVADKSXLVIQWRDTWARRXRKFQQREKKGKCKK 603
BLAST of netrin 1 vs. UniProt/SwissProt
Match: sp|Q2HXW4|NET1_PIG (Netrin-1 OS=Sus scrofa OX=9823 GN=NTN1 PE=2 SV=1) HSP 1 Score: 328.176 bits (840), Expect = 6.305e-102 Identity = 198/635 (31.18%), Postives = 321/635 (50.55%), Query Frame = 2
Query: 197 GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHI------NCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMA---------QAPENRCQHCNNKRKRIR--FKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGL 2026
G RC+P F N ++STCG+ + V +CH C K H +LTD + +N+T W S+ ++ NV +T S GK FE+ Y+SL P+S+ I KS+D G +W + ++S C + + N + IT+ +Q CT DS S ++AF+T + +H D W++AT+IR+ F+R++ + + + + S F+A++D+ +GGRC+C GHA RC+++ D++ CDC+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +GY RD KP++H C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ KIP+A + PE+ +C + +++ KKYC+KD + + + ++ G+ +F + + VY+ S+ + +WI+ D+ CKCP ++ YL++ +A ++S ++ D+ S+ + W+ TW RR++KF +E + L
Sbjct: 46 GHPRRCIPDFVNAAFGKDV-RVSSTCGRPPARYCVVSERGEERLRSCHLCNASDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIYKSMDYGRTWVPFQFYSTQCRKMY-------NRPHRAPITKQN-EQEAVCT---DSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVA-------------------------FSRLH-------------TFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHAARCVRD-RDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYFRDLGKPITHRKACKACDCHPVGAAG-KTCNQTTGQCPCKDGVTGVTCNRCAKGYQQSRSPIAPCI------KIPVAPPTTAASSVEEPEDCDSYCKASKGKLKINMKKYCKKDYAVQIHILKADKAGDWWKFTVNIISVYKQGASRIRRGDQN-------------------LWIRSRDIACKCPKIKPLKKYLLL--GNAEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKEL 600
BLAST of netrin 1 vs. TrEMBL
Match: Q49BF9 (Netrin 1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 1399.03 bits (3620), Expect = 0.000e+0 Identity = 668/669 (99.85%), Postives = 668/669 (99.85%), Query Frame = 2
Query: 83 MNQSLRNGICPFVIFAILIQCQAQHWLHGGSNSLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWKLKRRYYRRQKHTKNYV 2089
MNQSLRNGICPFVIFAILIQCQAQHWLHGGSNSLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQ ICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWKLKRRYYRRQKHTKNYV
Sbjct: 1 MNQSLRNGICPFVIFAILIQCQAQHWLHGGSNSLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQWICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWKLKRRYYRRQKHTKNYV 669
BLAST of netrin 1 vs. TrEMBL
Match: Q8WPG7 (Netrin 1 (Fragment) OS=Dugesia japonica OX=6161 GN=Djnet1 PE=2 SV=1) HSP 1 Score: 582.408 bits (1500), Expect = 0.000e+0 Identity = 269/325 (82.77%), Postives = 294/325 (90.46%), Query Frame = 2
Query: 1115 DCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWKLKRRYYRRQKHTKNYV 2089
+CE+CR GFVDKRW++A +NSA ECKRCNCNLHSKKC F+EKLYI+SN+QSGGVCVDCEHNTDGRYCH C KGYHRDWSKPLSH HVC+KCRCHPIGS+YPD CDQRNSQC CK GV GLSCNRCQKGFQQT+SPI PCV+ DLHKIPMAQAPEN+CQ CNNKRKRIRFKKYCRKDAVLLVTMQSREQHG+M RFEM+VN+VYRIDT KF+E +PDFLASMNGI+SNQF HSTFPMWIKE DLKCKCPSL LGMTYLV+LKLH IKYLERSELLLDQKSVALPWQKTWERRMKKF++KE QGLC KWKLKR Y + + + YV
Sbjct: 1 NCEVCRIGFVDKRWTIAAINSAGECKRCNCNLHSKKCSFDEKLYILSNRQSGGVCVDCEHNTDGRYCHVCKKGYHRDWSKPLSHQHVCLKCRCHPIGSVYPDKCDQRNSQCMCKPGVTGLSCNRCQKGFQQTRSPIHPCVETYDLHKIPMAQAPENKCQQCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGDMVRFEMRVNHVYRIDTLKFSETNPDFLASMNGIISNQFSHSTFPMWIKEADLKCKCPSLLLGMTYLVVLKLHTIKYLERSELLLDQKSVALPWQKTWERRMKKFASKEQQGLCVKWKLKRSYRHQYRQSNRYV 325
BLAST of netrin 1 vs. TrEMBL
Match: G4VPQ4 (Putative netrin OS=Schistosoma mansoni OX=6183 GN=Smp_146840 PE=4 SV=1) HSP 1 Score: 459.144 bits (1180), Expect = 4.765e-148 Identity = 257/644 (39.91%), Postives = 370/644 (57.45%), Query Frame = 2
Query: 176 NSLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHINCHSC----MKDTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLE-PDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIP-DSKLGNLVNSPSVLAFTTNNPNFFSHA-SDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRK--NNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMS----NQFIHST---------FPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWK 2041
NSLC S ++F+C PPF +I + TSTCG+++ Q +C + NC C K S YLTD+H N T WAS ++ G+ VN+T S GK FE+YYISL + P SI I KS D G +W WHYFS DCYR+FG+ P H +S IT + + + L P +S + V S V+AF+T + S WM+ T++RI+L +F S+ + RI R N + L VN + P +L DF+A ADI+IGGRC+C GHA C+ + C C+H+T+G+DCE C+ G++D+ W AT A CK+C+CNLHS +C F+ LY++SN+ SGG+C +C+HNT GR CHQC +GY+RDW+KP+SH +VC+ CRCHPIGSI CD+R+ QC CK GV GL+C+RCQ G+ QT+SP+ PC + C C+ ++RI+ KK+CRKDAV + +SRE H MARFEM + ++RI+ F + + + NQ H P+W++ +LKCKCP ++LG++YL++ + R+ELL K+ LPW+ +W+RR+ +F +E++G CEK++
Sbjct: 71 NSLCQSGSQSFQCAPPFKDISRG-VSVYSTSTCGEHQPQNLCRSNTNCQLCSVNSTKWKFSSEYLTDRHDVENQTCWASGYIRPGEAVNLTISLGKRFEVYYISLQPCSIGSIPHSIAIYKSSDFGRTWRPWHYFSTDCYRAFGL-PTSNEH--NSHITSANLQEVLCVALQPRESYYSHQVPSDWVIAFSTTLGRPATRPWSPALIDWMTMTDVRISL----MSFHQSNEPEY-----------RIKRNAYRNSQTDLKVNIRKSVNVTIDPKILSEIDFYAFADIAIGGRCKCNGHASECILGS-NGKLICACEHHTSGEDCEYCKPGYMDRPWDRATTQDANVCKKCDCNLHSNECRFSNSLYLLSNRISGGICENCQHNTIGRNCHQCAEGYYRDWTKPISHENVCLPCRCHPIGSIVRHDCDRRSGQCRCKQGVAGLTCDRCQDGYHQTRSPLNPCT------------KGDINCPPCSTNKERIKLKKFCRKDAVFEASFKSRELHENMARFEMHITQIWRINKEFFKGSSSIYWPASKSFIDFEQHNQGEHEEEIRRKSNELVPVWVRLNELKCKCPYIELGVSYLIVTDFESFTNKIRNELLFSTKTAILPWRSSWKRRLIRFRRRENRGACEKFR 682
BLAST of netrin 1 vs. TrEMBL
Match: A0A3Q0KP82 (Putative netrin OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 449.514 bits (1155), Expect = 4.074e-143 Identity = 266/717 (37.10%), Postives = 384/717 (53.56%), Query Frame = 2
Query: 176 NSLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHINCHSC----MKDTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLE-PDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPD----SKLGNLVNS--------------------PS--VLAFTTNNPNFFSHA-SDQYNSWMSATNIRITLR----------KSETNFLNSHLDGFTYPPHLKQYFNRI------YRKNNYSSI-LPVNRKLK---------QKKRN-----------------------SPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPEN-RCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMS----NQFIHST---------FPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWK 2041
NSLC S ++F+C PPF +I + TSTCG+++ Q +C + NC C K S YLTD+H N T WAS ++ G+ VN+T S GK FE+YYISL + P SI I KS D G +W WHYFS DCYR+FG+ P H +S IT + + + L P S G V S PS V+AF+T + S WM+ T++RI+L + + N S L + Q N++ Y +N++ L R L+ QK+ N P +L DF+A ADI+IGGRC+C GHA C+ + C C+H+T+G+DCE C+ G++D+ W AT A CK+C+CNLHS +C F+ LY++SN+ SGG+C +C+HNT GR CHQC +GY+RDW+KP+SH +VC+ CRCHPIGSI CD+R+ QC CK GV GL+C+RCQ G+ QT+SP+ PC + +A P + C C+ ++RI+ KK+CRKDAV + +SRE H MARFEM + ++RI+ F + + + NQ H P+W++ +LKCKCP ++LG++YL++ + R+ELL K+ LPW+ +W+RR+ +F +E++G CEK++
Sbjct: 71 NSLCQSGSQSFQCAPPFKDISRG-VSVYSTSTCGEHQPQNLCRSNTNCQLCSVNSTKWKFSSEYLTDRHDVENQTCWASGYIRPGEAVNLTISLGKRFEVYYISLQPCSIGSIPHSIAIYKSSDFGRTWRPWHYFSTDCYRAFGL-PTSNEH--NSHITSANLQEVLCVALQPRESYYSHQGRRVRSMNSYIFNKPNITRNFGGMGVPSDWVIAFSTTLGRPATRPWSPALIDWMTMTDVRISLMSFHQSNEPEYRIKRNAYRSQLSDHSKLTQNSQTINKLPLFQSNYFYDNFNKYQLKRTRNLRDGQTITELPQKEMNHTNNIDSQTDLKVNIRKSVNVTIDPKILSEIDFYAFADIAIGGRCKCNGHASECILGS-NGKLICACEHHTSGEDCEYCKPGYMDRPWDRATTQDANVCKKCDCNLHSNECRFSNSLYLLSNRISGGICENCQHNTIGRNCHQCAEGYYRDWTKPISHENVCLPCRCHPIGSIVRHDCDRRSGQCRCKQGVAGLTCDRCQDGYHQTRSPLNPCTKDFTSRAVALAHTPGDINCPPCSTNKERIKLKKFCRKDAVFEASFKSRELHENMARFEMHITQIWRINKEFFKGSSSTYWPASKSFIDFEQHNQGEHEEEIRRKSNELVPVWVRLNELKCKCPYIELGVSYLIVTDFESFTNKIRNELLFSTKTAILPWRSSWKRRLIRFRRRENRGACEKFR 782
BLAST of netrin 1 vs. TrEMBL
Match: A0A4Z2DC78 (Netrin-B isoform 1 OS=Schistosoma japonicum OX=6182 GN=EWB00_002313 PE=4 SV=1) HSP 1 Score: 438.728 bits (1127), Expect = 4.567e-139 Identity = 258/708 (36.44%), Postives = 378/708 (53.39%), Query Frame = 2
Query: 212 CVPPFTNIIENMIPDEITSTCGQNKKQRICVDHINCHSCMKDT----HSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLE-PDSIIISKSIDNGTSWTEWHYFSKDCYRSFGI-----------------------QPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHA-SDQYNSWMSATNIRITL----------------------------RKSETNF--------------LNSH--------LDGFTYPPHLKQYFNR--IYRKNNYSSIL---PVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPEN-RCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFL-ASMNGIMSNQFIHSTF------------PMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWK 2041
C PPF +I + TSTCG+++ Q +C +C C ++ S +YLTD+H NN T WAS + G+ VN+T S GK FE+YYISL + P SI I KS D G +W WHYFS DCYR+FG+ QP + + + S + ++ ++ G V S V+AF+T + S WM+ T++RI+L ++S N LN H DG + +Q N + + NY S+L P+N + SP VL DF+A ADI+IGGRC+C GHA C+ + + C C+H+T+G DCE C+ G++D+ W AT A CK+C+CNLHS +C F+ LY++SN+ SGGVC +C+HNT GR CHQC +GY+RDW+KP+SH +VC+ CRCHPIGSI CD+R+ QC CK GV GL+C+RCQ G+ QT+SP+ PC + +A P + C C+ ++RI+ KK+CRKDAV + +SRE HG MARFEM + ++RI+ + AS + I ++ H + P+W++ +LKCKCP ++LG++YL++ + R+ELL K+ LPW+ +W+RR+ +F +E++G CEK++
Sbjct: 85 CSPPFKDISRG-VTVHATSTCGEHQPQSLCRSSTDCQLCSVNSTEWKFSSDYLTDRHNINNQTCWASGHIQPGEVVNLTISLGKRFEVYYISLQPCSIGSMPHSIAIYKSSDFGRTWRPWHYFSTDCYRAFGLPTSNEHSIHITSANLQEVLCVALQPRESYYNHQGRKVRSMNNYIFNKPNVTNNFGGIGVPSDWVIAFSTTLGRPVTRPWSPALIDWMTMTDVRISLMNFHQNNEPEYRTKRTAHQNQLPHYSKLKQSSQNINRLPLFQSNYFYENLNKHQPKRTRSSRDGHSGNELARQEINHTNMMQNQNYLSVLTKQPLNATV------SPEVLSEIDFYAFADIAIGGRCKCNGHASECVLSS-NGKLTCACEHHTSGDDCEYCKPGYMDRPWDRATPQDANICKKCDCNLHSNECRFSNSLYLLSNRVSGGVCENCQHNTVGRNCHQCAEGYYRDWTKPISHENVCLPCRCHPIGSIVRHDCDRRSGQCRCKQGVAGLTCDRCQDGYHQTRSPLNPCTKDFTSRAVALAHTPGDINCPPCSTNKERIKLKKFCRKDAVFQASFKSRELHGNMARFEMYITQIWRINKEFLKGSSSIYWPASKSFIDFERYDHDEYDEEIQRKSNELVPVWVRLNELKCKCPYIELGISYLIVTDFESFTNKVRNELLFSIKTAILPWRSSWKRRLIRFRRRENRGACEKFR 784
BLAST of netrin 1 vs. Ensembl Cavefish
Match: NTN1 (netrin 1 [Source:HGNC Symbol;Acc:HGNC:8029]) HSP 1 Score: 349.747 bits (896), Expect = 3.663e-111 Identity = 214/648 (33.02%), Postives = 320/648 (49.38%), Query Frame = 2
Query: 164 HGGSNSLCYSE-GKAFRCVPPFTNIIENMIPDEI--TSTCGQNKKQRICVDHIN------CHSC----MKDTHSINYLTDQHLNNNITFWASDFVNHG--DNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPE------NRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
G S CY E G A RC+P F N E+ +STCG R C CH+C K H +YLTD + +N+T W S+ NV +T S GK FE+ Y+S+ PDS+ I KS+D G +W + ++S C + F N + + IT+ +Q CT D+ S ++AF+T + +H D W++AT++R+T + T P P + S F+A++D+ +GGRC+C GHA RC+K CDC+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +GY+RD SKP+SH C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ + A +PE + C+ K K I KKYC+KD + V + ++ GE +F + + VY+ S+ +Q +W++ D+ CKCP ++ G YL++ +S ++ D+ S+ + W+ TW RR++KF +E +G C+K
Sbjct: 30 QGSSPDPCYDEHGAARRCIPDFVNA---AFGKEVRASSTCGLKGPSRYCAQDKGEEGARTCHTCDASDPKRAHPGSYLTDLNNPHNLTCWQSETGVQALQGNVTLTLSLGKKFEVTYVSVQFCSK-RPDSMAIFKSMDYGKTWVPFQFYSTQCRKVF-------NRPNRAAITKQN-EQEAVCT---DAHADGRPLSGGLVAFSTLDGRPSAHDFDNSPVLQDWVTATDVRVTFSR------------LTAP-------------------------------GEPDEDEDSLFYAVSDLQVGGRCKCNGHASRCVKERDTGGLVCDCKHNTAGPECDRCKPFHYDRPWQRATAREAHECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDLSKPISHRKACKACDCHPVGAAG-KTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTTASSPEEPADCDSYCKASKGKLK-INMKKYCKKDYAVQVHILKSDKAGEWWKFTVNIISVYKQGESRIRR-------------GDQL------LWVRAKDVACKCPKIKTGKKYLLLGNDE--DSPGQSGVVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 596
BLAST of netrin 1 vs. Ensembl Cavefish
Match: NTN1 (netrin 1 [Source:HGNC Symbol;Acc:HGNC:8029]) HSP 1 Score: 350.132 bits (897), Expect = 2.432e-109 Identity = 213/646 (32.97%), Postives = 318/646 (49.23%), Query Frame = 2
Query: 164 HGGSNSLCYSE-GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHIN------CHSC----MKDTHSINYLTDQHLNNNITFWASDFVNHG--DNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPE------NRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
G S CY E G A RC+P F N +STCG R C CH+C K H +YLTD + +N+T W S+ NV +T S GK FE+ Y+S+ PDS+ I KS+D G +W + ++S C + F N + + IT+ +Q CT D+ S ++AF+T + +H D W++AT++R+T + T P P + S F+A++D+ +GGRC+C GHA RC+K CDC+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +GY+RD SKP+SH C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ + A +PE + C+ K K I KKYC+KD + V + ++ GE +F + + VY+ S+ +Q +W++ D+ CKCP ++ G YL++ +S ++ D+ S+ + W+ TW RR++KF +E +G C+K
Sbjct: 207 QGSSPDPCYDEHGAARRCIPDFVNAAFGK-EVRASSTCGLKGPSRYCAQDKGEEGARTCHTCDASDPKRAHPGSYLTDLNNPHNLTCWQSETGVQALQGNVTLTLSLGKKFEVTYVSVQFCSK-RPDSMAIFKSMDYGKTWVPFQFYSTQCRKVF-------NRPNRAAITKQN-EQEAVCT---DAHADGRPLSGGLVAFSTLDGRPSAHDFDNSPVLQDWVTATDVRVTFSR------------LTAP-------------------------------GEPDEDEDSLFYAVSDLQVGGRCKCNGHASRCVKERDTGGLVCDCKHNTAGPECDRCKPFHYDRPWQRATAREAHECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDLSKPISHRKACKACDCHPVGAAG-KTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTTASSPEEPADCDSYCKASKGKLK-INMKKYCKKDYAVQVHILKSDKAGEWWKFTVNIISVYKQGESRIRR-------------GDQL------LWVRAKDVACKCPKIKTGKKYLLLGNDE--DSPGQSGVVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 773
BLAST of netrin 1 vs. Ensembl Cavefish
Match: ntn1b (netrin-1-like [Source:NCBI gene;Acc:111191060]) HSP 1 Score: 344.354 bits (882), Expect = 9.277e-109 Identity = 207/644 (32.14%), Postives = 325/644 (50.47%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNIIENMIPDEI--TSTCGQNKKQRICV-------DHINCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPM---------AQAPENRCQHCNNKRKRIR--FKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLC 2029
CY E G+ RC+P F N E+ +STCG R CV CH+C K H YLTD + +N+T W S+ +V H NV +T S GK FE+ Y+SL P+S+ I KS+D G +W + ++S C + F N S + IT+ +Q CT DS + ++AF+T + +H D W++AT++R+T F+R++ + + + + S F+A++D+ +GGRC+C GHA +C+K+ C+C+HNTAG +C+ C+ D+ W AT A EC C+CNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +GY+RD +K +SH C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ KIP+ A+ P + HC + +++ KKYC+KD + V + ++ GE +F + + VY+ + +Q +W++ D+ CKCP ++ G YL +L +S ++ D+ S+ +PW+ W RR++KF ++ +G C
Sbjct: 57 CYDENGQPRRCIPDFVNA---AFGKEVRASSTCGAQTAARYCVVTERGEERSRECHTCDAADPKKAHPAAYLTDLNNPHNLTCWQSESYVQHPQNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIYKSMDYGKTWVPFQFYSTQCKKMF-------NRPSRAAITKQN-EQEAVCT---DSHTDMRPLAGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDVRVT-------------------------FSRLH-------------TFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASKCVKDRDGGGLVCECKHNTAGAECDRCKPFHYDRPWQRATAREANECVACHCNLHARRCRFNMELYKLSGRRSGGVCLNCRHNTAGRHCHYCKEGYYRDMTKAISHRRACKACDCHPVGAA-GKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCI------KIPVASPTATYTSAEEPTDCDSHCKASKGKMKVTMKKYCKKDFAVQVHVLKGDKAGEWWKFTINIISVYKQGEHRIRR-------------GDQL------LWVRAKDVACKCPKIKPGKKYL-LLGSDDDDSRGQSGVVADKGSLLIPWKDLWARRLRKFQQRDKRGKC 620
BLAST of netrin 1 vs. Ensembl Cavefish
Match: NTN1 (netrin 1 [Source:HGNC Symbol;Acc:HGNC:8029]) HSP 1 Score: 272.707 bits (696), Expect = 1.038e-80 Identity = 186/642 (28.97%), Postives = 284/642 (44.24%), Query Frame = 2
Query: 164 HGGSNSLCYSE-GKAFRCVPPFTNIIENMIPDEI--TSTCGQNKKQRICVDHIN------CHSC----MKDTHSINYLTDQHLNNNITFWASDFVNHG--DNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
G S CY E G A RC+P F N E+ +STCG R C CH+C K H +YLTD + +N+T W S+ NV +T S GK FE+ Y+S+ PDS+ I KS+D G +W + ++S C + F N + + IT+ +Q CT D+ S ++AF+T + +H D W++AT++R+T + T P P + S F+A++D+ +GGRC+C GHA RC+K CDC+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +GY+RD SKP+SH C + P + + + CK G L +I KKYC+KD + V + ++ GE +F + + VY+ S+ +Q +W++ D+ CKCP ++ G YL++ +S ++ D+ S+ + W+ TW RR++KF +E +G C+K
Sbjct: 207 QGSSPDPCYDEHGAARRCIPDFVNA---AFGKEVRASSTCGLKGPSRYCAQDKGEEGARTCHTCDASDPKRAHPGSYLTDLNNPHNLTCWQSETGVQALQGNVTLTLSLGKKFEVTYVSVQFCSK-RPDSMAIFKSMDYGKTWVPFQFYSTQCRKVF-------NRPNRAAITKQN-EQEAVCT---DAHADGRPLSGGLVAFSTLDGRPSAHDFDNSPVLQDWVTATDVRVTFSR------------LTAP-------------------------------GEPDEDEDSLFYAVSDLQVGGRCKCNGHASRCVKERDTGGLVCDCKHNTAGPECDRCKPFHYDRPWQRATAREAHECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDLSKPISHRKACKEIPVAPPTTTASSPEEPADCDSYCKASKGKL----------------------------------------------KINMKKYCKKDYAVQVHILKSDKAGEWWKFTVNIISVYKQGESRIRR-------------GDQL------LWVRAKDVACKCPKIKTGKKYLLLGNDE--DSPGQSGVVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 723
BLAST of netrin 1 vs. Ensembl Cavefish
Match: ntn1b (netrin-1-like [Source:NCBI gene;Acc:111191060]) HSP 1 Score: 265.774 bits (678), Expect = 1.390e-79 Identity = 177/633 (27.96%), Postives = 286/633 (45.18%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNIIENMIPDEI--TSTCGQNKKQRICV-------DHINCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLC 2029
CY E G+ RC+P F N E+ +STCG R CV CH+C K H YLTD + +N+T W S+ +V H NV +T S GK FE+ Y+SL P+S+ I KS+D G +W + ++S C + F N S + IT+ +Q CT DS + ++AF+T + +H D W++AT++R+T F+R++ + + + + S F+A++D+ +GGRC+C GHA +C+K+ C+C+HNTAG +C+ C+ D+ W AT A EC C+CNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +GY+RD +K +SH C + + Y + + CK G + ++ KKYC+KD + V + ++ GE +F + + VY+ + +Q +W++ D+ CKCP ++ G YL +L +S ++ D+ S+ +PW+ W RR++KF ++ +G C
Sbjct: 57 CYDENGQPRRCIPDFVNA---AFGKEVRASSTCGAQTAARYCVVTERGEERSRECHTCDAADPKKAHPAAYLTDLNNPHNLTCWQSESYVQHPQNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIYKSMDYGKTWVPFQFYSTQCKKMF-------NRPSRAAITKQN-EQEAVCT---DSHTDMRPLAGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDVRVT-------------------------FSRLH-------------TFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASKCVKDRDGGGLVCECKHNTAGAECDRCKPFHYDRPWQRATAREANECVACHCNLHARRCRFNMELYKLSGRRSGGVCLNCRHNTAGRHCHYCKEGYYRDMTKAISHRRACKEIPVASPTATYTSAEEPTDCDSHCKASKGKM----------------------------------------------KVTMKKYCKKDFAVQVHVLKGDKAGEWWKFTINIISVYKQGEHRIRR-------------GDQL------LWVRAKDVACKCPKIKPGKKYL-LLGSDDDDSRGQSGVVADKGSLLIPWKDLWARRLRKFQQRDKRGKC 570
BLAST of netrin 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009682.1 (pep scaffold:Pmarinus_7.0:GL479835:738:11671:-1 gene:ENSPMAG00000008750.1 transcript:ENSPMAT00000009682.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 153.295 bits (386), Expect = 1.851e-41 Identity = 109/366 (29.78%), Postives = 170/366 (46.45%), Query Frame = 2
Query: 446 GKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQ--SLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQT-KSPITPCV 1534
GK F+I Y+ L P+S I K WT + Y+S C ++G + F D + T D+ ++ + + L PS F N+P W+++T IR+TL + L + + ++ N VL+ S ++A++D ++GGRC+C GHA C+KN + C C+HNT G DCE C + D+ W AT SA EC C+CN + +C F+ +LY + GG C +C NTDG+ C +C + ++R + S H C+ C C+P+GS Y CD++ +C+CK GV G C++C GF ++ PC
Sbjct: 1 GKAFDITYVRLKFH-TSRPESFAIYKRASMDGPWTPYQYYSGSCEPTYGKRNRGFLRSGDDEQTPLCTDEFSDISPLTGGNVAFSTLEGRPSAYNFD-NSPVL--------QDWVTSTEIRVTLNR------------------LNTFGDEVF--------------------NDHKVLR-SYYYAISDFAVGGRCKCNGHASECVKNEL-GALACKCRHNTMGADCEKCLPLYNDRPWHRATSESANECTLCDCNGRADECYFDPELYRATGH--GGHCTNCRDNTDGKTCERCLENFYR---RSASEH--CLPCNCNPVGS-YSRQCDEQ-GRCSCKQGVMGERCDQCLPGFHSMDEAGCRPCT 307
BLAST of netrin 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008925.1 (pep scaffold:Pmarinus_7.0:GL476496:30277:70426:-1 gene:ENSPMAG00000008054.1 transcript:ENSPMAT00000008925.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 120.168 bits (300), Expect = 3.504e-28 Identity = 96/375 (25.60%), Postives = 154/375 (41.07%), Query Frame = 2
Query: 449 KLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFS---KDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTY--PPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEF-------NEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVC-----------DQRNSQCTCKTGVGGLSCNRCQKGFQ 1504
++F++ Y+ + P + I+ +S+D G ++ W Y+S +C +GI P N D + CTS L ++ + N S + + SA IR+ L++ T LN+ L ++ P + R Y ++++ DIS+GG C C GHA C N C C+HNT G++C+ C G+ +RW T+N+ C++CNC+ ++ C + N L + GGVCV+C NT G C C G++R + + C C+C P GS+ VC D QC C+ G G C RC ++
Sbjct: 1 QVFQVAYVIVKAANSPRPGNWILERSLD-GATYHPWQYYSVSDSECLTRYGITPTVGNPVYRR-------DDEVICTSYYVVLL--MLARGKIHTSLINGRPSADDPSPKLLDFTSARYIRLRLQRIRT--LNADLMTLSHRDPREVDPIVTRRY------------------------------YYSIKDISVGGMCICSGHASTCPWNEDTQKMECQCEHNTCGENCQHCCPGYNQRRWRPGTINNGNTCEKCNCHGKTEDCYYDAEVDRTNRSLSVHGRFSGGGVCVNCSANTAGTNCETCRDGFYRPTGVLPNDPYPCRLCQCDPQGSLS-QVCIKDEKHADPEKDLSPGQCLCRPGFAGERCERCAFAYR 332 HSP 2 Score: 65.4698 bits (158), Expect = 3.224e-11 Identity = 61/219 (27.85%), Postives = 81/219 (36.99%), Query Frame = 2
Query: 1016 GGRCQ---CYGHAGRCLKNPVDNTYH-CDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLH----------------------------SKKCEFNEKLYIVSNKQSGGVCVDCEHN-------------------------TDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGF 1501
GGRC+ C+GHA C D C+HNT G CE+CR GF + AT +A +C C C L +CE + GG C CE N T G +C +C G++ D + C C+CHP G+ P CD+ N QC C+ V G +C +C GF
Sbjct: 619 GGRCEPCRCHGHADDCDDLSGDCMLPLSGCRHNTMGPHCELCRPGF----YGNATRGTADDCLPCTCPLSIASNNFSPTCHQDPRGVLTCDQCLPGYIGLRCERCADDFFGEPSSPGGSCRRCECNGNEEAWGGGRVCDARTGQCLRCRERTAGFHCERCADGFYGDATGTGG----CQPCQCHPEGATAPQ-CDRINGQCPCRPSVVGRTCEQCAIGF 828 HSP 3 Score: 58.151 bits (139), Expect = 6.930e-9 Identity = 50/152 (32.89%), Postives = 61/152 (40.13%), Query Frame = 2
Query: 1076 TYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNL-HSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGS-----IYPDV-----CDQRNSQCTCKTGVGGLSCNRCQKG 1498
T C C NT G C C +D W V N A C+ CNC+ HS+ +E + G C C GR C +C +G W P C C CHP G+ P CD+R QC CK VGG C+ C G
Sbjct: 900 TGRCLCPPNTEGTRCHRC----IDDHWGV---NPHAGCRACNCSAAHSRGARCDE---------ASGQC-SCLDGYGGRTCGECAQGR---WGYP-----ACRPCECHPEGTRANTCPAPPTGSLCGCDERTGQCACKENVGGTRCDACLPG 1026 HSP 4 Score: 54.299 bits (129), Expect = 8.997e-8 Identity = 50/173 (28.90%), Postives = 69/173 (39.88%), Query Frame = 2
Query: 1016 GGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKG-FQQTKSPITPC 1531
GGR C G+CL+ C+ TAG CE C +GF C+ C C+ +++ N Q C + GR C QC G++ LS C C CHP+G+ V + +C C GV G SC+RC G + + TPC
Sbjct: 748 GGR-VCDARTGQCLR----------CRERTAGFHCERCADGFY------GDATGTGGCQPCQCHPEGATAPQCDRI----NGQ-----CPCRPSVVGRTCEQCAIGFYG-----LSSGAGCSPCPCHPVGTA--GVACSADGRCHCWPGVEGRSCDRCTSGHYGFKEGGCTPC 887
BLAST of netrin 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004606.1 (pep scaffold:Pmarinus_7.0:GL477874:5526:15188:-1 gene:ENSPMAG00000004198.1 transcript:ENSPMAT00000004606.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 95.1301 bits (235), Expect = 1.212e-22 Identity = 64/249 (25.70%), Postives = 106/249 (42.57%), Query Frame = 2
Query: 446 GKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQ--SLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAE 1186
GK F+I Y+ L P+S I K + W + ++S C R+FG+ F D + T D+ ++ + + L P LAF N+ W++AT++R++LR+ L + + ++ P VL+ S ++A++D+++GGRC+C+GHA C + D C CQHNT G DCE C + W+ A+ + A E
Sbjct: 3 GKAFQITYVRLRFH-TSRPESFAIYKRTSPASPWQPYQFYSGTCERTFGLPSRGFLRAGDEERTALCSDEFSDISPLTGGNVAFSTLEGRPGALAFDGND--------KLQQEWVTATDVRVSLRR------------------LNTFGDEVF--------------------GDPKVLR-SYYYAVSDLAVGGRCKCHGHASECGRG-SDGRLVCRCQHNTTGDDCERCLPSHNSRPWAQASSDDAHE 202
BLAST of netrin 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004614.1 (pep scaffold:Pmarinus_7.0:GL477874:5547:15173:-1 gene:ENSPMAG00000004198.1 transcript:ENSPMAT00000004614.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 82.0333 bits (201), Expect = 2.873e-18 Identity = 62/242 (25.62%), Postives = 101/242 (41.74%), Query Frame = 2
Query: 455 FEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQ-----SLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVA 1165
F+I Y+ L P+S I K + W + ++S C R+FG+ F D + T D+ LT ++ S L L +S L+ T W++AT++R++LR+ L + + ++ P VL+ S ++A++D+++GGRC+C+GHA C + D C CQHNT G DCE C + W+ A
Sbjct: 1 FQITYVRLRF-HTSRPESFAIYKRTSPASPWQPYQFYSGTCERTFGLPSRGFLRAGDEERTALCSDEFSDISPLTGGNVAFSTLEGLFHSTGPLSSATQ-------------EWVTATDVRVSLRR------------------LNTFGDEVF--------------------GDPKVLR-SYYYAVSDLAVGGRCKCHGHASECGRGS-DGRLVCRCQHNTTGDDCERCLPSHNSRPWAQA 188
BLAST of netrin 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007784.1 (pep scaffold:Pmarinus_7.0:GL477336:382472:417638:1 gene:ENSPMAG00000007035.1 transcript:ENSPMAT00000007784.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 84.7297 bits (208), Expect = 3.736e-17 Identity = 73/300 (24.33%), Postives = 111/300 (37.00%), Query Frame = 2
Query: 425 VNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDS---KLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSN---KQSGGVCVDCEHNTDG 1306
V+I G Y I L P +P+ + I + G+ W +W F+KDC + Y+ SK S+ C P GN+ + T PN YN++ + ++ + +E L L+G +L V R + A++++ + GRC+C+GHA RC + C Q NT G +CE+CR F DK W + C+ C C + C + L + GGVC DC NT G
Sbjct: 109 VSIELRGGSYQVFYVIVQFLSP--QPEGVRIQRRPAAGSPWVDWQVFAKDC----------VSMYNMSKNGPLEKPDSVNCLQFPREVPLSRGNVT-----FSVLTPEPNL----RPGYNAFYATPALQAFVLAAEVRVL---LEG---------------------RLLGVAGVAGGPLR---------PYHAVSEVIVSGRCECHGHAERCDSSTTPYRCLCSAQSNTEGPNCELCRPLFRDKPWRAGDTRNQNACRPCLCYGRALSCRYEAILDPHPGLHWRGGGGVCQDCTQNTTG 354
BLAST of netrin 1 vs. Ensembl Nematostella
Match: EDO36836 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHI5]) HSP 1 Score: 295.434 bits (755), Expect = 2.389e-91 Identity = 197/624 (31.57%), Postives = 307/624 (49.20%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICV-----DHINCHSC----MKDTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNS--PSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNN-KRKRIRFKKYCRKDAVLLVTMQSREQH--GEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKF 2002
CY+E +A C P F ++ N ++ TCG+ K +R C C C K +H YLTD H NN+T W +D V +NV + SFGK ++I Y+SL + PDS+ I KS+D G SW + ++S C + +G ++ +I+ +Q CT G+L+ +AF+T + D W+ AT+I++ FN I R S P + S ++A+++ ++GGRC C GHA C+ + + CDC+HNT G DC CR F D+ W AT +SA ECK CNCNLH++ C F+ +L+ +S +SGGVCV C HNT GRYCH C +G+++D +K ++H VC +C CHP GS VC+Q QC CK GV G SC++C G++ TKS I PC++ ++ + P +R Q C+ +R ++ K++CRKD L VT+ E+ R + +N +++ ++ ++ WIK+ D +C+CP L+ G +Y + L + ++L+ +SV LPW + +R+ +F
Sbjct: 5 CYNEFNRAQLCQPAFHDLAYNK-DVYVSHTCGE-KSRRFCPPMSVRGRTACGFCDERNAKTSHPAKYLTDVHNENNVTCWQADPVQSRENVTLILSFGKTYDITYVSLQFCGI-RPDSMGIYKSMDYGKSWQAYQFYSSRCEKMYG-------RVTNGEISPEN-EQVALCTD------GHLLRPLYGGRIAFSTLEGRPSASKMDYSAVMQEWVMATDIKVE-------------------------FNMISR-----SARP----------------RDSLYYAVSEFNVGGRCHCNGHASECVSD-RNGRQVCDCKHNTVGVDCGACRPFFNDRPWRPATHHSANECKMCNCNLHARTCVFSMELFKLSGGRSGGVCVKCRHNTAGRYCHYCREGFYKDPNKHITHIKVCRQCNCHPHGS-RGRVCNQSTGQCPCKEGVVGRSCDKCAPGYKSTKSNIVPCIETPNVVMSQESSTPRSRDQGCSQCRRVPLKVKRFCRKDFALKVTVVRVEKKLPNSWVRATVNINTLFKRSRTRRVRRGDGYV------------------WIKKRDFRCRCPKLKPGRSYFIAGNLRGQS--RKRSIVLNGRSVVLPWNEKLLKRIYRF 543
BLAST of netrin 1 vs. Ensembl Nematostella
Match: EDO36523 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIF3]) HSP 1 Score: 175.252 bits (443), Expect = 1.906e-45 Identity = 133/468 (28.42%), Postives = 210/468 (44.87%), Query Frame = 2
Query: 185 CY-SEGKAFRCVPPFTN------IIENMIPDEITSTCGQNKKQRICVD------HINCHSCMKDTHSINY----LTDQHLNNNITFWASDFV--NHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFS-HASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPV---DNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQK---GFQQT 1510
CY S+G+A RC+P F N +I N +TCG K + CV C C ++ + +TD + N T+W S+ + N V +T GK +++ YI + P+S+ I K + W + YFS C +++G+ + + I + Q CT S + L + + P+ ++ S W++A +RI LR+ T + + ++ P+VL+ S ++A+ D+SIGGRC+C GHA C + + C CQH+T G DCE C+ + D+ W+ A+ +A EC RCNCN ++ C F+E LY + GG C +C+ NTDG C +C + ++R S C+ C C+P+GS+ QC CK GV G+ C++CQ GF QT
Sbjct: 21 CYDSQGRAQRCMPQFGNAAFGKRVISN-------NTCGDPPK-KFCVQTGVLEVRKECEMCNSSDPALEHPALKITDIKDDTNRTWWQSETLLENPDKPVTLTLDLGKSYDVSYIR-IRFRSPRPESMAIYKKTTPSSKWIPYQYFSATCEKTYGV--------TTNGIVDPRNQQVALCTDAF-STITPLTGGNVAFSTLEDRPDAYNFDNSPALQEWVTAVAVRIHLRRMNT------------------FGDEVF--------------------GDPNVLR-SYYYAIIDLSIGGRCKCNGHASECEETRLWDGQTRLQCKCQHDTEGVDCERCKPLYNDRPWARASSRNANECVRCNCNGLAESCVFDEALYRQTGH--GGRCQNCKQNTDGPNCERCKENFYRKTS-----LEPCVACGCNPVGSVSLQC--NSEGQCRCKPGVTGIKCDKCQANYFGFSQT 422 HSP 2 Score: 64.6994 bits (156), Expect = 1.108e-10 Identity = 44/144 (30.56%), Postives = 63/144 (43.75%), Query Frame = 2
Query: 1025 CQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDV---CDQRNSQC 1447
CQCYGH+ C +PV T C+C+HNT G C+ C +G+ + T + +CK+C C ++ + + VC DC G C C GY+ D C KC C G+I P+ CD+ C
Sbjct: 704 CQCYGHSDTC--DPV--TGKCNCKHNTTGIRCDKCIDGY----YGNPTQGTPDDCKKCPCKFGTRCIQIGANV----------VCTDCPEGHVGNLCDMCQDGYYGDPMGKRGAPTTCQKCNCS--GNIDPNAIGSCDRLTGDC 827 HSP 3 Score: 55.4546 bits (132), Expect = 7.900e-8 Identity = 53/184 (28.80%), Postives = 78/184 (42.39%), Query Frame = 2
Query: 1025 CQCY--------GHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKS---PITPCVDVI 1543
C+CY GH L P D+ C C N +G C+ CR + W+VA S C+ CNC+ +N + + G C C+ GR C QC+ ++ C+ C C+ GS+ CD++ C C+ GV G+ C+ CQ+ F + PC DVI
Sbjct: 864 CECYVQGTVSPPGH-DPTLPFPCDDKGRCTCLPNVSGDKCDTCRPEY----WNVA---SGKGCEPCNCDTTGS---WNNTCSVTT-----GQC-HCKPGVTGRRCDQCSIDHY------AFSELGCLACNCNLQGSLNV-TCDEQGV-CQCRPGVLGIKCDSCQENFHNLTAGCIACPPCYDVI 1022
BLAST of netrin 1 vs. Ensembl Nematostella
Match: EDO39502 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9X8]) HSP 1 Score: 173.711 bits (439), Expect = 6.685e-45 Identity = 117/389 (30.08%), Postives = 171/389 (43.96%), Query Frame = 2
Query: 389 FWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSF-GIQPMDFNHYSDSKITE--STVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYP-PHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTY------HCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRN--------SQCTCKTGVGGLSCNRCQKGF 1501
+W S+ N +NV + F ++ ++ P ++I +S D G W + YFS DC + F GI + D T STV+ S N + + + S + + + TNIRI K L++ D P P +K+ + ++A+ D+ I G C CYGHA +CL P T C+C HNT GK+CE C++G+ D W A ++ EC++CNCN H+ KC F+ +Y S SGGVC DCEHNT GR C +C +++D SK S CI C C P G + C+ +N +C CK VGG+ C+RC+ GF
Sbjct: 97 WWQSE--NGRENVYLQLDLESEFHFTHL-IMRFRTFRPAGMVIERSWDYGKKWQIYRYFSADCDKIFPGIPKGEPKDIDDVICTNKYSTVEPS--------------TNGEVIFRALKSQIPIKNAYSSKVQNLLKITNIRINFTK-----LHTLGDDLLDPRPEIKRKY----------------------------------YYAVYDLVIRGSCSCYGHAEQCLPEPGQPTTPGMVYGRCNCTHNTTGKNCERCQDGYFDVPWRPADADNPNECRKCNCNGHAVKCRFDPAVYQASGNVSGGVCQDCEHNTVGRNCERCKPLHYQDPSKKFSDPDACIACNCDPTGGVNGGECEAKNDPAAGLVAGRCICKRNVGGVRCDRCKAGF 429 HSP 2 Score: 87.0409 bits (214), Expect = 1.781e-17 Identity = 61/165 (36.97%), Postives = 80/165 (48.48%), Query Frame = 2
Query: 1025 CQCYGHAGRCLKNPVDNTYHC-DCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYI-VSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDV---CDQRNSQC-TCKTGVGGLSCNRCQKGF 1501
C+CYGHA C NP T C DCQHNT GK+CE C GF + +AT + +CKRC C S +F+E Y+ S +C C+ GR C +C GY + PL C C C+ +I P V CD+ C C G +C RC+ G+
Sbjct: 850 CRCYGHASSC--NP--KTGVCVDCQHNTVGKNCEACAVGF----YGIATKGTPNDCKRCQCPGGSSGNKFSESCYVDTSTTPPSVLCDKCQVGYTGRQCEECADGY---YGNPLVPGGSCKPCSCN--NNIDPTVSGNCDRLTGSCLQCLYNTAGFNCERCKPGY 1001 HSP 3 Score: 53.5286 bits (127), Expect = 2.994e-7 Identity = 40/139 (28.78%), Postives = 56/139 (40.29%), Query Frame = 2
Query: 1082 HCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKG 1498
C C+ N G C+ C+ GF + T ++ CK C CN E N G C C+ +G C +C Y S P C C C +G Y + CD++ QC C+ G+ G C R + G
Sbjct: 410 RCICKRNVGGVRCDRCKAGFFN-----LTTDNPDGCKACGCNPLGTIGECNPDT---------GRCT-CKRYVEGEDCDRCASEYWGLSSTPAG----CSNCACD-VGGSYDNQCDKQTGQCRCRKGITGRQCERVKTG 528 HSP 4 Score: 53.1434 bits (126), Expect = 4.100e-7 Identity = 41/137 (29.93%), Postives = 62/137 (45.26%), Query Frame = 2
Query: 1091 CQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGF 1501
C +NTAG +CE C+ G+ A C C C+ N + N ++G +C + +G+ C +C +G+ W+ LS C +C C GS+ +C+Q QC CK G GG C C+ F
Sbjct: 985 CLYNTAGFNCERCKPGYFGDAI-------ARNCTSCVCDKKGT----NPGFHYSCNHKTGQC--NCLPDVEGKQCDRCAEGF---WN--LSSGKGCQQCDCCIEGSLR-SMCNQITGQCQCKAGFGGPRCCECEDNF 1102 HSP 5 Score: 51.2174 bits (121), Expect = 1.723e-6 Identity = 39/138 (28.26%), Postives = 61/138 (44.20%), Query Frame = 2
Query: 1076 TYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRC 1489
T C+C + GK C+ C EGF W+++ S C++C+C + L + N+ +G C+ G C +C + W P C C C+P GS++ CD+ +C CK G G C+ C
Sbjct: 1032 TGQCNCLPDVEGKQCDRCAEGF----WNLS---SGKGCQQCDCCIEGS-------LRSMCNQITGQC--QCKAGFGGPRCCECEDNF---WGSPPDG---CSACNCNPAGSVHLQ-CDRDTGKCLCKVGHIGDKCDMC 1146
BLAST of netrin 1 vs. Ensembl Nematostella
Match: EDO36501 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIF6]) HSP 1 Score: 165.622 bits (418), Expect = 3.206e-42 Identity = 130/448 (29.02%), Postives = 191/448 (42.63%), Query Frame = 2
Query: 263 TSTCGQNKKQRICVDHIN-CHSCMKDT----HSINYLTDQHLNNNITFWASDF------VNHGDN--VNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMD--FNHYSD--SKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTT--NNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAE-CKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGF-QQTKSPITPCVDVI 1543
TSTCG + V +N C C + H I Y+ D+ ++ TFW S+ N D+ VN++ SF K ++I + P I+ KS D G +W Y+S +C + FG++ D N + D +K TE S P N F N +FF QY +SAT++R+ + T+ L D T LKQY+ +A++D+ + RC+C+GHA C P + +CDC HNT G DCE C F +K W AT + C+RC C+ H+ C + S ++ G C DC HNT+G +C +C ++R+ S L+H +C C C P G C+Q QC C+ V G C+ C F T PI C + +
Sbjct: 50 TSTCGSPPSEYCRVKPLNGCFKCNASSPTERHPIEYIADKSPGSDATFWQSETWWDWYQKNPRDDLKVNVSISFNKTYDITGHIMFTFESPRPSKAILEKSFDKGQTWDPLQYYSFNCRKDFGMENTDPTENRFGDFSAKCTEKYSSSS------PQQ------NGKVTFDFLRRYNKASFFDTQVQQY---LSATDLRLRMEYPGTDGLEVAKDEVT----LKQYY-----------------------------------YAVSDLEVIARCKCHGHARWC-DFPHETGKNCDCLHNTTGDDCERCLPLFNNKTWMPATSETEPNPCERCECHSHADSCTY-------SQQEGYGTCDDCYHNTEGHFCERCKDKFYRNMSLSLNHPEICPDCNCFPQGITNNGTCNQTTGQCECRANVTGRQCDSCADTFWGYTLEPIGYCTECL 435 HSP 2 Score: 57.3806 bits (137), Expect = 2.356e-8 Identity = 74/343 (21.57%), Postives = 131/343 (38.19%), Query Frame = 2
Query: 527 IDNGTSWT-EWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASD---QYNSWMSATNIRITLRKSETNFLNSHLDGFTYP-PHLKQYFNRIYRKNNYSSILPVNRK---LKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQH-NTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHR--DWSKPLSHH-------HVCIKCRCHPIGSIYPD--VCDQRNSQCTCKTGVGGLSCNRCQK 1495
+D G+ ++ +W + +D + Q +F+ + +T LG VN FT N N + + +Y+S N+++ +TN D P P + Y+ R++ N P R L++ + S+ + F + + + + + + + + +C C+ N G CE+C +GF R + NS + C C C S C+ G C C + G YC QC G D ++ L + + C C C ++Y + VC+ QCTCK + G +C+RC +
Sbjct: 953 VDLGSPFSIDWTFVQRDVSMARVSQGRNFSFVTSGNMT-----------------LGTKVN------FTKNQLNSYGELLEMKLKYSS-QGPLNVKMFWELEKTNGGIVRFDVKPTPSPEVTSYYVRLHEANGIWKTEPNRRNASDLQELLSDLMSITLNAQFMSNGSVLLS-EIKLHSASQGIVGEKAGHVENCTCESGNYTGLSCELCNQGFTRNRPN----NSYSACSLCRCTGRSLDCDGET-----------GQCKGCRLGSQGLYCEQCQPGVDNTTDCTQCLPEYWGLKEGSNGCQDCDCVLPNTLYGNSTVCNATTGQCTCKEKISGRTCDRCHE 1255 HSP 3 Score: 52.373 bits (124), Expect = 7.481e-7 Identity = 48/206 (23.30%), Postives = 73/206 (35.44%), Query Frame = 2
Query: 1022 RCQCYGHAGRCLKNPVDNTYHCD-CQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNC----------------------NLHSKKCEFNEKLYIVSNKQSGGVCVDC-----------------------EHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGF 1501
RC+C+ HA C + + CD C HNT G CE C++ F R ++N C CNC N+ ++C+ + + G C +C + N R C +C GY W P + C +C C GS+ +C + C C+T + G C G+
Sbjct: 325 RCECHSHADSCTYSQQEGYGTCDDCYHNTEGHFCERCKDKFY--RNMSLSLNHPEICPDCNCFPQGITNNGTCNQTTGQCECRANVTGRQCDSCADTFWGYTLEPIGYCTECLCVGNGTVNASFNCDQYNGQCSCKPNVHTRTCQECKHGY---WDFPKTLDTDCKRCDCDYGGSVL-QICSKTTGSCMCRTNIIGTRCTDVTTGY 524
BLAST of netrin 1 vs. Ensembl Nematostella
Match: EDO46760 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNY3]) HSP 1 Score: 128.257 bits (321), Expect = 3.002e-30 Identity = 110/416 (26.44%), Postives = 169/416 (40.62%), Query Frame = 2
Query: 341 HSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRC--LKNPVDNTYHCDCQHN----TAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQS---GGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIG-SIYPDVCDQRNS-------QCTCKTGVGGLSCNRCQKGFQQTKSP-ITPCV 1534
H Y+TD + T+W S ++ V + + +L + I L P P++I+I +S D G+++ W YF+ DC SFG++ GNLVN S+ HA ++T K NS G F + + +N I N L Q +++ +FAL + + GRC+C+GHA + +K + C C+ + T G+ CE C + +K + T + C +C CN H+ C +N L ++ GGVC++C HNT G++CH C + R+ K LS VC C+C G C + +S QC CK G C+ C+ GF KS T CV
Sbjct: 332 HPSEYMTDA---DTTTYWQSAVTDNA-TVEVDLLYQELQIFFVIMLFYGP--PPEAILIERSRDRGSTYKPWQYFASDCQSSFGME-----------------------------NNGNLVNPDSINCIQYQRCECHGHAQ------------KVTFIKG-----NSSSPGRCRCECDPSTFTQGEKASNVR-IQLYNHTLIQNEKHR--------YFALKRVLVTGRCECHGHAQKVTFIKGNSSSPGRCRCECDPSTFTQGEKCESCLPMYNNKAFLRGTNDDPYPCVKCECNNHATACYYNSSLDPSPASRTVGGGGVCINCLHNTAGQFCHVCRDDFFREPGKSLSAVDVCSPCQCTGPGVETGKTSCARDDSTPGVIAGQCECKQYTQGRQCDACKPGFYDLKSSHSTGCV 686 HSP 2 Score: 87.0409 bits (214), Expect = 1.785e-17 Identity = 61/192 (31.77%), Postives = 84/192 (43.75%), Query Frame = 2
Query: 986 DFF-----ALADISIGGRCQCYG---HAGRCLKNPVDNTY-----HCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYH--RDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKS 1516
DFF +L+ + + CQC G G+ D+T C+C+ T G+ C+ C+ GF D + S +T C C CN N S QS G C C+ N GR C C G++ RD + P C C C P G++ VCD + QC CK G GL C+ C+ G+ S
Sbjct: 610 DFFREPGKSLSAVDVCSPCQCTGPGVETGKTSCARDDSTPGVIAGQCECKQYTQGRQCDACKPGFYDLKSSHST-----GCVTCGCNTAGT---VNRN---ASCDQSRGQCY-CKSNVTGRTCDTCESGFYGLRD-NDPSG----CRACECDPFGTLPGSVCDAGSGQCRCKAGSTGLRCHLCEDGYHSLDS 784 HSP 3 Score: 56.9954 bits (136), Expect = 3.049e-8 Identity = 36/141 (25.53%), Postives = 63/141 (44.68%), Query Frame = 2
Query: 1082 HCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDV-CDQRNSQCTCKTGVGGLSCNRCQKGF 1501
C C+ + G C +C +G+ +++S+ CK CNC+ K + + ++ G C C+ +G+ C +C GY ++ + C C C P G + C+ + CTCK V +C +C KG+
Sbjct: 760 QCRCKAGSTGLRCHLCEDGY-------HSLDSSG-CKTCNCSTIGAKPGL-----VNTCDKTNGQCY-CKTYVEGKTCDRCKTGY---YNLTTVNQDGCSLCSCDPKGIQGSSLTCEVSSGNCTCKKYVREPTCGQCMKGY 883 HSP 4 Score: 53.1434 bits (126), Expect = 4.336e-7 Identity = 39/150 (26.00%), Postives = 58/150 (38.67%), Query Frame = 2
Query: 1082 HCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPC 1531
+C C+ C C +G+ +++++ N C+ CNCN + S G C C N G C QC Y+ + S + C C C GS+ + C+ C CK +GG C+RC G PC
Sbjct: 864 NCTCKKYVREPTCGQCMKGY----FNLSSSNPEG-CQACNCNPKGTLGGDRTPAGELECSGSNGKCA-CLTNVQGLRCDQCVSAYYWNPSG-----YGCSPCNCDASGSLATN-CNS-TGYCQCKPNIGGRRCDRCMPGSYGGPGSCKPC 1000
BLAST of netrin 1 vs. Ensembl Medaka
Match: NTN1 (netrin-1 [Source:NCBI gene;Acc:101155405]) HSP 1 Score: 349.747 bits (896), Expect = 5.000e-111 Identity = 210/640 (32.81%), Postives = 330/640 (51.56%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHI--------NCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDV-IDLHKIPMAQAP-----ENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
CY E G RC+P F N +++STCG+ + V NCHSC K H YLTD + +N+T W S+ F+ + NV +T S GK FE+ Y+SL P+S++I KS+DNG +W + ++S C + + N + + IT+ +Q CT DS + ++AF+T + +H D W++AT+I++ F+R++ + + + + S F+A++D+ +GGRC+C GHA RC+K+ D + CDC+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +G++RD SKP+SH C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ + + P + E+ C+ K K I KKYC+KD + V + ++ GE +F + + VY+ S+ +QF +W++ D+ CKCP ++ G YL++ +S ++ D+ S+ + W+ TW RR++KF +E +G C+K
Sbjct: 38 CYDEHGNPRRCIPDFVNAAFGK-EVKVSSTCGKTPSRYCVVTSAEKGEERTRNCHSCDASDPKKYHPPAYLTDLNNPHNLTCWQSENFIQYPQNVTLTLSLGKKFEVTYVSLQFCSP-RPESMVIYKSMDNGKTWVPFQFYSTQCRKMY-------NKPNRAVITKQN-EQEAVCT---DSHTDMYPLTGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVV-------------------------FSRLH-------------TFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASRCVKD-RDGSLVCDCKHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDLSKPISHRKACKACDCHPVGAA-GKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVSPPTTPASSTEEASDCESYCKASKGKLK-INMKKYCKKDYAVQVHILKADKMGEWWKFTVNIISVYKQGESRIRR-------------GDQF------LWVRAKDVACKCPKIKPGKKYLLLGNDEVSP--SQSGVVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 602
BLAST of netrin 1 vs. Ensembl Medaka
Match: NTN1 (netrin-1 [Source:NCBI gene;Acc:101155405]) HSP 1 Score: 342.813 bits (878), Expect = 9.804e-109 Identity = 205/628 (32.64%), Postives = 324/628 (51.59%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDV-IDLHKIPMAQAP-----ENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
CY E G RC+P F N +++STCG+ S K H YLTD + +N+T W S+ F+ + NV +T S GK FE+ Y+SL P+S++I KS+DNG +W + ++S C + + N + + IT+ +Q CT DS + ++AF+T + +H D W++AT+I++ F+R++ + + + + S F+A++D+ +GGRC+C GHA RC+K+ D + CDC+HNTAG +C+ C+ D+ W AT A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +G++RD SKP+SH C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ + + P + E+ C+ K K I KKYC+KD + V + ++ GE +F + + VY+ S+ +QF +W++ D+ CKCP ++ G YL++ +S ++ D+ S+ + W+ TW RR++KF +E +G C+K
Sbjct: 38 CYDEHGNPRRCIPDFVNAAFGK-EVKVSSTCGKTCDA----------SDPKKYHPPAYLTDLNNPHNLTCWQSENFIQYPQNVTLTLSLGKKFEVTYVSLQFCSP-RPESMVIYKSMDNGKTWVPFQFYSTQCRKMY-------NKPNRAVITKQN-EQEAVCT---DSHTDMYPLTGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVV-------------------------FSRLH-------------TFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASRCVKD-RDGSLVCDCKHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDLSKPISHRKACKACDCHPVGAA-GKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVSPPTTPASSTEEASDCESYCKASKGKLK-INMKKYCKKDYAVQVHILKADKMGEWWKFTVNIISVYKQGESRIRR-------------GDQF------LWVRAKDVACKCPKIKPGKKYLLLGNDEVSP--SQSGVVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 580
BLAST of netrin 1 vs. Ensembl Medaka
Match: ntn1b (netrin-1 [Source:NCBI gene;Acc:101161061]) HSP 1 Score: 330.102 bits (845), Expect = 1.453e-103 Identity = 205/642 (31.93%), Postives = 324/642 (50.47%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICV-------DHINCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMA---------QAPENRCQHC--NNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLC 2029
CY E G RC+P F N ++STCG N R CV +CH+C K H YLTD + +N+T W S+ +V + NV +T S GK FE+ Y+SL P+S+ I KS+D G SW + ++S C + + N + + IT+ +Q CT DS + ++AF+T + +H D W++AT+I++ F+R++ + + + + S F+A++D+ +GGRC+C GHA +C+K+ N C+C+HNTAG +C+ C+ D+ W AT A EC C+CNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +GY RD SKP+SH C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ KIP+A + P + +C + + +I KKYC+KD + V + ++ GE +F + + VY+ + +Q +W++ D+ C+CP ++ G YL++ S ++ D+ S+ +PW+ W RR++KF ++ +G C
Sbjct: 40 CYDENGNPRRCIPDFVNSAFGKDV-RVSSTCG-NPAARFCVVSEKGEERTRDCHTCDSNDPKKAHPPAYLTDLNNPHNLTCWQSETYVQYPQNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIYKSMDYGKSWVPFQFYSTQCKKMY-------NRQNKATITKQN-EQEAICT---DSHTDMHPLTGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVI-------------------------FSRLH-------------TFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASKCVKDREGNLV-CECKHNTAGPECDRCKPFHYDRPWQRATAREANECVACHCNLHARRCRFNMELYKLSGRRSGGVCLNCRHNTAGRHCHYCKEGYFRDMSKPISHRRACKACDCHPVGAA-GKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCI------KIPVASPTSTYSSYEEPSDCDSYCKASKGKMKITMKKYCKKDYAVQVHVLKGDKAGEWWKFTVNIISVYKQGRHRIRR-------------GDQL------LWVRAKDVACRCPKIKPGRKYLLLGTDD--DSPGHSGVVADKGSLLIPWKDLWGRRLRKFQQRDKRGKC 600
BLAST of netrin 1 vs. Ensembl Medaka
Match: ntn1b (netrin-1 [Source:NCBI gene;Acc:101161061]) HSP 1 Score: 330.102 bits (845), Expect = 1.462e-103 Identity = 205/642 (31.93%), Postives = 324/642 (50.47%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICV-------DHINCHSC----MKDTHSINYLTDQHLNNNITFWASD-FVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMA---------QAPENRCQHC--NNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLC 2029
CY E G RC+P F N ++STCG N R CV +CH+C K H YLTD + +N+T W S+ +V + NV +T S GK FE+ Y+SL P+S+ I KS+D G SW + ++S C + + N + + IT+ +Q CT DS + ++AF+T + +H D W++AT+I++ F+R++ + + + + S F+A++D+ +GGRC+C GHA +C+K+ N C+C+HNTAG +C+ C+ D+ W AT A EC C+CNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +GY RD SKP+SH C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SPI PC+ KIP+A + P + +C + + +I KKYC+KD + V + ++ GE +F + + VY+ + +Q +W++ D+ C+CP ++ G YL++ S ++ D+ S+ +PW+ W RR++KF ++ +G C
Sbjct: 41 CYDENGNPRRCIPDFVNSAFGKDV-RVSSTCG-NPAARFCVVSEKGEERTRDCHTCDSNDPKKAHPPAYLTDLNNPHNLTCWQSETYVQYPQNVTLTLSLGKKFEVTYVSLQFCSP-RPESMAIYKSMDYGKSWVPFQFYSTQCKKMY-------NRQNKATITKQN-EQEAICT---DSHTDMHPLTGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVI-------------------------FSRLH-------------TFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASKCVKDREGNLV-CECKHNTAGPECDRCKPFHYDRPWQRATAREANECVACHCNLHARRCRFNMELYKLSGRRSGGVCLNCRHNTAGRHCHYCKEGYFRDMSKPISHRRACKACDCHPVGAA-GKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCI------KIPVASPTSTYSSYEEPSDCDSYCKASKGKMKITMKKYCKKDYAVQVHVLKGDKAGEWWKFTVNIISVYKQGRHRIRR-------------GDQL------LWVRAKDVACRCPKIKPGRKYLLLGTDD--DSPGHSGVVADKGSLLIPWKDLWGRRLRKFQQRDKRGKC 601
BLAST of netrin 1 vs. Ensembl Medaka
Match: ntn2 (netrin-3 [Source:NCBI gene;Acc:101160359]) HSP 1 Score: 322.398 bits (825), Expect = 2.361e-100 Identity = 208/659 (31.56%), Postives = 318/659 (48.25%), Query Frame = 2
Query: 185 CYSE-GKAFRCVPPFTNII---ENMIPDEITSTCGQNKKQRICV-----DHINCHSCM-------KDTHSINYLTDQHLNNNITFWASDFVNHGD-NVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ---YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISD----FFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRC---QHCNNKRK--RIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKY-------------LERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEK 2035
CY E G A RC+P F N E M+ +S CG+ + V + + +C + H +YLTD + +N+T W S+ +N NV +T S GK FEI Y+SL P+S+ I KS+D G +W + ++S C R + N + + IT+ ++L CT D S ++AF+T + S D W++ T+IR+ + P L + F NN R+ V S F+A+ D +GGRC+C GHA RCLK+ + CDC+HNT G +C+ C+ D+ W A A EC CNCNLH+++C FN +LY +S ++SGGVC++C HNT GR+CH C +G++RD +P++H C C CHP+G+ C+Q QC CK GV G++CNRC KG+QQ++SP+ PC+ + ++ + + E +C + +I KKYC+KD + V + E G+ A+F + + VY+ + P L + I+ W+ DL CKCP +Q+ +LV+ ER L+ D+ S+ + W+ W RR++KF KE +G C K
Sbjct: 37 CYDETGTARRCIPEFINAAFGKEVMV----SSVCGRPPSRSCSVVERGDERPSVRTCQICDAADPRRAHPASYLTDLNSAHNLTCWQSENLNTSPHNVTLTLSLGKKFEITYVSLQFCSP-RPESLAIYKSMDYGKTWMPYQFYSSQCRRMY-------NKPNKATITKQNEQEAL-CT---DGHTDLYPLSGGLIAFSTLDGRPSSKDFDNSPVLQDWVTVTDIRVVFGR----------------PQLSREFVLGAGSNNGG-------------RDDDPVAVTSTLSTYFYAVGDFQVGGRCKCNGHASRCLKDK-EGKLVCDCKHNTEGPECDRCKPFHYDRPWQRANAREANECLPCNCNLHARRCRFNMELYKLSGRKSGGVCMNCRHNTAGRHCHYCKEGFYRDMGRPITHRRACKACDCHPVGAAG-KTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPVAPCIKIPVVNPTAVVSSTEEPADCESYCKPVKGNLKINMKKYCKKDYAVQVNVLDMETVGDWAKFSVSLVSVYK------SRGEP--LKRGDNIL-----------WVHMKDLACKCPKIQMSKKFLVMGGSEGGTGPAAGPGVGATSPGAERVGLVADKNSLVIQWRDVWSRRLRKFQRKEKKGKCSK 629
BLAST of netrin 1 vs. Planmine SMEST
Match: SMESG000006806.1 (SMESG000006806.1) HSP 1 Score: 1399.8 bits (3622), Expect = 0.000e+0 Identity = 668/669 (99.85%), Postives = 668/669 (99.85%), Query Frame = 2
Query: 83 MNQSLRNGICPFVIFAILIQCQAQHWLHGGSNSLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWKLKRRYYRRQKHTKNYV 2089
MNQSLRNGICPFVIFAILIQCQAQHWLHGGSNSLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCV CEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWKLKRRYYRRQKHTKNYV
Sbjct: 1 MNQSLRNGICPFVIFAILIQCQAQHWLHGGSNSLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVGCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWKLKRRYYRRQKHTKNYV 669
BLAST of netrin 1 vs. Planmine SMEST
Match: SMESG000006806.1 (SMESG000006806.1) HSP 1 Score: 774.237 bits (1998), Expect = 0.000e+0 Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 2
Query: 83 MNQSLRNGICPFVIFAILIQCQAQHWLHGGSNSLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKR 1195
MNQSLRNGICPFVIFAILIQCQAQHWLHGGSNSLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKR
Sbjct: 1 MNQSLRNGICPFVIFAILIQCQAQHWLHGGSNSLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKR 371
BLAST of netrin 1 vs. Planmine SMEST
Match: SMESG000006806.1 (SMESG000006806.1) HSP 1 Score: 627.476 bits (1617), Expect = 0.000e+0 Identity = 297/298 (99.66%), Postives = 297/298 (99.66%), Query Frame = 2
Query: 1196 CNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWKLKRRYYRRQKHTKNYV 2089
CNCNLHSKKCEFNEKLYIVSNKQSGGVCV CEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWKLKRRYYRRQKHTKNYV
Sbjct: 19 CNCNLHSKKCEFNEKLYIVSNKQSGGVCVGCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWKLKRRYYRRQKHTKNYV 316
BLAST of netrin 1 vs. Planmine SMEST
Match: SMESG000069610.1 (SMESG000069610.1) HSP 1 Score: 451.055 bits (1159), Expect = 1.992e-150 Identity = 254/626 (40.58%), Postives = 359/626 (57.35%), Query Frame = 2
Query: 179 SLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHINCHSCMKDTHSINYLTDQHLNNNITFWASDFVNHGDNVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTTNNPNFFSHASDQ-YNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPD-FLASMNGIMSNQFIHS--TFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQ-KSVALPWQKTWERRMKKFSNKEHQGLCEKWK 2041
S CY+ G A++C+PPFTNI+ENMIP +I + C KK+ +C +K N +TD HL +N T W S + ++VNITFSF K FE+YYISL P++I KS + G +W WHY + +C F ++ + +Q+ C + KL N P V+AF T + + D WMSA++IRITL K + + P + + RNSP + +++DISIGGRC+C GHA C KNP+ N C CQHNT G+DCE C++ ++D W+ ATVNS A+CK+C CNLHS C F++ LYI+ + +GGVC +C+HNT G CH CN GY+RDW+KPL H VC KC CH IG+ +PD CD+R QC CK+GV G CNRC GF+Q+ S TPCV I + ++ EN+C+ C++KR RIRF+K+CRKDAV VT+QS+E G+ RF++ V+ ++ S +++P F+ S+ S +++ T P+W+K DLKC C LQLG TYL I+K Y R+ELL+ +S+ L W TW+RR+ +F K+ +G C K++
Sbjct: 7 SPCYAHGSAYQCMPPFTNIVENMIP-KIETLC---KKKEVCP--------IKSPRD-NLITDYHLTHNQTCWKSVPL-QANHVNITFSFAKRFEVYYISLTPCESGIPNAIAFYKSNNFGITWKPWHYLATNCLSMFNMKETTIPSLQFNDFRIQRREQAARCFPL---KLSLTDNEP-VVAFNTVLEGAYENVGDSSLIDWMSASDIRITLFK-------------------------------FDNPKPKKSNIFRFARNSPKKI-----LSISDISIGGRCKCNGHANACNKNPITNKIECVCQHNTEGQDCEKCKKNYLDLPWARATVNSPAQCKKCECNLHSSNCYFSQDLYILRKQITGGVCTNCQHNTAGVRCHYCNDGYYRDWTKPLDHKFVCKKCTCHIIGAKFPDRCDKRTGQCLCKSGVTGEMCNRCASGFRQSNSTETPCVKYIQM------ESAENKCKACDSKRTRIRFRKFCRKDAVFSVTLQSQEIIGDFIRFDVTVDNIWHSKNS-IKDLYPGIFIESIMRPSSKSRLNNRPTNPIWLKLRDLKCNCVQLQLGQTYLFIIKSTTYHYQNRAELLIKSPRSIILIWNPTWKRRLNRFKQKKEKGECSKFQ 571
BLAST of netrin 1 vs. Planmine SMEST
Match: SMESG000050895.1 (SMESG000050895.1) HSP 1 Score: 419.853 bits (1078), Expect = 4.041e-137 Identity = 236/653 (36.14%), Postives = 358/653 (54.82%), Query Frame = 2
Query: 158 WLHG------GSNSLCYSEGKAFRCVPPFTNIIENMIPDEITSTCGQNKKQRICVDHIN---------CHSCMKDTHSI--NYLTDQHLNNNITFWASDFV--NHGDN-VNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQPMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFTT--NNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAECKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWKLKR 2050
W+H +S CY G+ +C+P FTNIIEN + E TSTCG N ++IC + + C C S +++TD+H+ N T W S + N G N VNIT S K FE+YYI+L + L PDSI I KS+D G SW W +FS+DCYR+F M + +S+IT + + + L + + + +VL F+T P+ + D WM+ T++RI+L + + P LK+ + ++ LP + P V F L+D+++GGRC+C GHA RCL + D CDC HNT G++CE C+ ++D+ W AT +A CK C+CNLHS +C FN ++Y+ +N +GGVC++C H+T G C C G+ R+W+KP H C C+CH IGSI + CD++ QC C+ GV G +C+RC+ G++Q+ S + PC+ + + C+ CN KRKRI FKK+CR+ +V T S+E HG M R+E+K++ ++R+D + + +FL M T+ WI D++C CP ++LG +YL++ + YL EL +D +SV LPW +WERR++KF +E +G CE++ K+
Sbjct: 15 WIHSVKVIFESQSSPCYLNGRPQQCLPSFTNIIEN-VKAETTSTCGTNGPEQICRNWYDKTTAEARQYCSICDAKHRSYPPSHITDRHIPKNQTCWFSGPLQENPGQNEVNITVSLKKSFEVYYIALQICGTL-PDSIAIYKSLDFGVSWKPWQFFSQDCYRAFK---MPTTNEQNSQITPANIHEVLCVELKAPERYNDYGQAETVLPFSTIYGRPSGPPWSQDLV-EWMTMTDLRISLMR--------FTPEKSTPYRLKELYY-------HTESLPQAPPNLVNHKTEPIV-----HFGLSDLAVGGRCKCNGHANRCLIDDNDEI-KCDCHHNTEGQECERCKSNYLDRPWQRATRQNANVCKYCDCNLHSYRCYFNSQIYVRNNYTNGGVCINCRHHTTGNNCENCENGFFRNWTKPQEHRAACTSCKCHTIGSIKQNECDKKTGQCYCRDGVTGQTCDRCKDGYKQSNSTVRPCIPSSGMADMANTSPV---CKKCNEKRKRIVFKKFCRRKSVFRATALSKEIHGNMIRYELKIDEIWRVDKNVW-----EFLPRETPAM------HTYRAWINSEDVRCNCPKVELGQSYLILTRAKYYLYLRNKELYIDSRSVMLPWHNSWERRLQKFQKREKRGKCEQYFKKK 626 The following BLAST results are available for this feature:
BLAST of netrin 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of netrin 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of netrin 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of netrin 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of netrin 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of netrin 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of netrin 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of netrin 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of netrin 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of netrin 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of netrin 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of netrin 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of netrin 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of netrin 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30013386 ID=SMED30013386|Name=netrin 1|organism=Schmidtea mediterranea sexual|type=transcript|length=2169bpback to top protein sequence of SMED30013386-orf-1 >SMED30013386-orf-1 ID=SMED30013386-orf-1|Name=SMED30013386-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=670bp MNQSLRNGICPFVIFAILIQCQAQHWLHGGSNSLCYSEGKAFRCVPPFTNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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