Arginine/serine-rich coiled-coil protein 2

Overview
NameArginine/serine-rich coiled-coil protein 2
Smed IDSMED30013337
Length (bp)1175
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Arginine/serine-rich coiled-coil protein 2 (SMED30013337) t-SNE clustered cells

Violin plots show distribution of expression levels for Arginine/serine-rich coiled-coil protein 2 (SMED30013337) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Arginine/serine-rich coiled-coil protein 2 (SMED30013337) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Arginine/serine-rich coiled-coil protein 2 (SMED30013337) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013337

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30013337SMESG000023487.1 SmedASXL_013626SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30013337SMESG000023487.1 SmedASXL_013626SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30013337SMESG000023487.1 dd_Smed_v4_1966_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30013337SMESG000023487.1 dd_Smed_v4_1966_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Human
Match: RSRC2 (arginine and serine rich coiled-coil 2 [Source:HGNC Symbol;Acc:HGNC:30559])

HSP 1 Score: 77.7962 bits (190), Expect = 5.402e-15
Identity = 54/122 (44.26%), Postives = 72/122 (59.02%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQ-STIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP K+AE E+KRK+L   KK+  K  + E W   +     G+  +  KFRKLMGI  E  A  S++D    K   +Q  +F  LDA Y+ ARS THT RG+GLG+  S
Sbjct:  310 TGIAVPSYYNPAAVNPMKFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKSEDEAGCSSVDEESYKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSS 427
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Celegans
Match: ZC262.2 (pep chromosome:WBcel235:III:8332223:8333572:1 gene:WBGene00022579.1 transcript:ZC262.2a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZC262.2)

HSP 1 Score: 49.2914 bits (116), Expect = 1.298e-6
Identity = 30/78 (38.46%), Postives = 42/78 (53.85%), Query Frame = 2 
Query:  614 NIEAWNASSVIASRG-DDKKAAKFRKLMGIHDEPN-AQSTIDLSKKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGY 841
            N + W  SS IA+ G D  KA KF +LMG+ + P     +  LS +++ Q  + N L+  Y  AR  TH  RG G G+
Sbjct:  156 NEKLW--SSAIAATGVDSSKANKFMRLMGVKNAPKPTDDSSRLSDEKNRQDKMLNDLEKQYAIARETTHMGRGTGFGF 231
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Zebrafish
Match: rsrc2 (arginine/serine-rich coiled-coil 2 [Source:NCBI gene;Acc:406566])

HSP 1 Score: 74.3294 bits (181), Expect = 3.423e-14
Identity = 50/120 (41.67%), Postives = 67/120 (55.83%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXX--XXXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQSTI----DLSKKEHEQAVLFNRLDADYQSARSHTHTSRGVGLG 838
            +G+ +P Y N +AVNP K+AE E+KRK+     K+ D+K    E W   +     G+  +  KFRKLMGI  E  A S+     D  K   +Q  +F  LD  Y+ ARS THT RG+GLG
Sbjct:  279 TGIAVPSYYNPSAVNPMKFAEQEKKRKMLWQGKKEGDNKSQTAELWEKLNF----GNKDQNVKFRKLMGIKGEEEAASSAAVNEDGLKTLQQQEEMFRNLDVQYEMARSQTHTQRGMGLG 394
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Xenopus
Match: ccnj (cyclin J [Source:Xenbase;Acc:XB-GENE-485597])

HSP 1 Score: 80.4925 bits (197), Expect = 5.991e-16
Identity = 53/122 (43.44%), Postives = 75/122 (61.48%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGI-HDEPNAQSTIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP ++AE E+KRK+L   KK+  K  + E W   +     G+  +  KFRKLMGI ++E  A S++D+   K   +Q  +F  LDA Y+ ARS THT RG+GLG+  S
Sbjct:  285 TGISVPSYYNPAAVNPMRFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKNEEEAATSSVDVESFKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSS 402
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Xenopus
Match: ccnj (cyclin J [Source:Xenbase;Acc:XB-GENE-485597])

HSP 1 Score: 80.1073 bits (196), Expect = 6.273e-16
Identity = 53/122 (43.44%), Postives = 75/122 (61.48%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGI-HDEPNAQSTIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP ++AE E+KRK+L   KK+  K  + E W   +     G+  +  KFRKLMGI ++E  A S++D+   K   +Q  +F  LDA Y+ ARS THT RG+GLG+  S
Sbjct:  285 TGISVPSYYNPAAVNPMRFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKNEEEAATSSVDVESFKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSS 402
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Xenopus
Match: ccnj (cyclin J [Source:Xenbase;Acc:XB-GENE-485597])

HSP 1 Score: 79.7221 bits (195), Expect = 6.904e-16
Identity = 53/122 (43.44%), Postives = 75/122 (61.48%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGI-HDEPNAQSTIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP ++AE E+KRK+L   KK+  K  + E W   +     G+  +  KFRKLMGI ++E  A S++D+   K   +Q  +F  LDA Y+ ARS THT RG+GLG+  S
Sbjct:  220 TGISVPSYYNPAAVNPMRFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKNEEEAATSSVDVESFKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSS 337
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Xenopus
Match: ccnj (cyclin J [Source:Xenbase;Acc:XB-GENE-485597])

HSP 1 Score: 79.7221 bits (195), Expect = 8.438e-16
Identity = 53/122 (43.44%), Postives = 75/122 (61.48%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGI-HDEPNAQSTIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP ++AE E+KRK+L   KK+  K  + E W   +     G+  +  KFRKLMGI ++E  A S++D+   K   +Q  +F  LDA Y+ ARS THT RG+GLG+  S
Sbjct:  289 TGISVPSYYNPAAVNPMRFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKNEEEAATSSVDVESFKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSS 406
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Mouse
Match: Rsrc2 (arginine/serine-rich coiled-coil 2 [Source:MGI Symbol;Acc:MGI:1913489])

HSP 1 Score: 75.485 bits (184), Expect = 1.733e-15
Identity = 54/122 (44.26%), Postives = 72/122 (59.02%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXXXXXXXXX-XXXNIEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQ-STIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP K+AE E+KRK+LW  K +  K  + E W   +     G+  +  KFRKLMGI  E  A  S++D    K   +Q  +F  LDA Y+ ARS THT RG+GLG+  S
Sbjct:   79 TGIAVPSYYNPAAVNPMKFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKSEDEAGCSSVDEESYKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSS 196
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Mouse
Match: Rsrc2 (arginine/serine-rich coiled-coil 2 [Source:MGI Symbol;Acc:MGI:1913489])

HSP 1 Score: 78.1814 bits (191), Expect = 2.769e-15
Identity = 54/122 (44.26%), Postives = 72/122 (59.02%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQ-STIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP K+AE E+KRK+L   KK+  K  + E W   +     G+  +  KFRKLMGI  E  A  S++D    K   +Q  +F  LDA Y+ ARS THT RG+GLG+  S
Sbjct:  310 TGIAVPSYYNPAAVNPMKFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKSEDEAGCSSVDEESYKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSS 427
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Mouse
Match: Rsrc2 (arginine/serine-rich coiled-coil 2 [Source:MGI Symbol;Acc:MGI:1913489])

HSP 1 Score: 77.411 bits (189), Expect = 3.225e-15
Identity = 54/122 (44.26%), Postives = 72/122 (59.02%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQ-STIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP K+AE E+KRK+L   KK+  K  + E W   +     G+  +  KFRKLMGI  E  A  S++D    K   +Q  +F  LDA Y+ ARS THT RG+GLG+  S
Sbjct:  252 TGIAVPSYYNPAAVNPMKFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKSEDEAGCSSVDEESYKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSS 369
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Mouse
Match: Rsrc2 (arginine/serine-rich coiled-coil 2 [Source:MGI Symbol;Acc:MGI:1913489])

HSP 1 Score: 77.411 bits (189), Expect = 3.225e-15
Identity = 54/122 (44.26%), Postives = 72/122 (59.02%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQ-STIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP K+AE E+KRK+L   KK+  K  + E W   +     G+  +  KFRKLMGI  E  A  S++D    K   +Q  +F  LDA Y+ ARS THT RG+GLG+  S
Sbjct:  252 TGIAVPSYYNPAAVNPMKFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKSEDEAGCSSVDEESYKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSS 369
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Mouse
Match: Rsrc2 (arginine/serine-rich coiled-coil 2 [Source:MGI Symbol;Acc:MGI:1913489])

HSP 1 Score: 77.411 bits (189), Expect = 3.541e-15
Identity = 54/122 (44.26%), Postives = 72/122 (59.02%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQ-STIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP K+AE E+KRK+L   KK+  K  + E W   +     G+  +  KFRKLMGI  E  A  S++D    K   +Q  +F  LDA Y+ ARS THT RG+GLG+  S
Sbjct:  263 TGIAVPSYYNPAAVNPMKFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKSEDEAGCSSVDEESYKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSS 380
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. UniProt/SwissProt
Match: sp|Q7ZYR8|RSRC2_XENLA (Arginine/serine-rich coiled-coil protein 2 OS=Xenopus laevis OX=8355 GN=rsrc2 PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 1.066e-15
Identity = 53/122 (43.44%), Postives = 73/122 (59.84%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGI-HDEPNAQSTIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP K+AE E+KRK+L   KK+  K  + E W   +     G+  +  KFRKLMGI H+E    S++D    K   +Q  +F  +DA Y+ ARS THT RG+GLG+  S
Sbjct:  226 TGISVPSYYNPAAVNPMKFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKHEEEAGTSSVDAESFKTLKQQEEMFRNMDAQYEMARSQTHTQRGMGLGFTSS 343
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. UniProt/SwissProt
Match: sp|Q5XHJ5|RSRC2_XENTR (Arginine/serine-rich coiled-coil protein 2 OS=Xenopus tropicalis OX=8364 GN=rsrc2 PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 3.174e-15
Identity = 53/122 (43.44%), Postives = 75/122 (61.48%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGI-HDEPNAQSTIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP ++AE E+KRK+L   KK+  K  + E W   +     G+  +  KFRKLMGI ++E  A S++D+   K   +Q  +F  LDA Y+ ARS THT RG+GLG+  S
Sbjct:  285 TGISVPSYYNPAAVNPMRFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKNEEEAATSSVDVESFKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSS 402
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. UniProt/SwissProt
Match: sp|A6QLS2|RSRC2_BOVIN (Arginine/serine-rich coiled-coil protein 2 OS=Bos taurus OX=9913 GN=RSRC2 PE=2 SV=2)

HSP 1 Score: 77.7962 bits (190), Expect = 2.210e-14
Identity = 54/122 (44.26%), Postives = 72/122 (59.02%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQ-STIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP K+AE E+KRK+L   KK+  K  + E W   +     G+  +  KFRKLMGI  E  A  S++D    K   +Q  +F  LDA Y+ ARS THT RG+GLG+  S
Sbjct:  311 TGIAVPSYYNPAAVNPMKFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKSEDEAGCSSVDEESYKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSS 428
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. UniProt/SwissProt
Match: sp|A2RTL5|RSRC2_MOUSE (Arginine/serine-rich coiled-coil protein 2 OS=Mus musculus OX=10090 GN=Rsrc2 PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.256e-14
Identity = 54/122 (44.26%), Postives = 72/122 (59.02%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQ-STIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP K+AE E+KRK+L   KK+  K  + E W   +     G+  +  KFRKLMGI  E  A  S++D    K   +Q  +F  LDA Y+ ARS THT RG+GLG+  S
Sbjct:  252 TGIAVPSYYNPAAVNPMKFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKSEDEAGCSSVDEESYKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSS 369
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. UniProt/SwissProt
Match: sp|Q5PQR4|RSRC2_RAT (Arginine/serine-rich coiled-coil protein 2 OS=Rattus norvegicus OX=10116 GN=Rsrc2 PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.362e-14
Identity = 54/122 (44.26%), Postives = 72/122 (59.02%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQ-STIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N AAVNP K+AE E+KRK+L   KK+  K  + E W   +     G+  +  KFRKLMGI  E  A  S++D    K   +Q  +F  LDA Y+ ARS THT RG+GLG+  S
Sbjct:  252 TGIAVPSYYNPAAVNPMKFAEQEKKRKMLWQGKKEGDKSQSAEIWEKLNF----GNKDQNVKFRKLMGIKSEDEAGCSSVDEESYKTLKQQEEVFRNLDAQYEMARSQTHTQRGMGLGFTSS 369
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. TrEMBL
Match: A0A1S8WTM2 (SMAP domain-containing protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_06588 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 6.271e-20
Identity = 61/127 (48.03%), Postives = 83/127 (65.35%), Query Frame = 2 
Query:  509 LPCYLNSAAVNPTKYAEIEQXXXXXXXXXXXXXXXNIEA--WNASSVIASRGDDKKAAKFRKLMGIHDEPNAQ-STIDLSKKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQSAFIDPHSYLA 880
            LP Y N  +VN  K AE ++KRKLLWSKK +    + +A  W  +S++A +GD   AAKFRKLMGIHD+P    S  D  K+  EQA LF RL+ +Y+ +R+ THT RGVGLG++ +  +D  +Y A
Sbjct:  158 LPKYYNPGSVNAVKLAEQQEKRKLLWSKKKEDNAQDTKASAWTTTSLVAGKGDSAAAAKFRKLMGIHDDPGQDGSQDDAQKRAEEQAELFRRLEYEYEQSRALTHTQRGVGLGFSSATHVDYGAYSA 284
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. TrEMBL
Match: A0A075A1D8 (SMAP domain-containing protein OS=Opisthorchis viverrini OX=6198 GN=T265_04027 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 1.973e-19
Identity = 61/127 (48.03%), Postives = 83/127 (65.35%), Query Frame = 2 
Query:  509 LPCYLNSAAVNPTKYAEIEQXXXXXXXXXXXXXXXNIEA--WNASSVIASRGDDKKAAKFRKLMGIHDEPNAQ-STIDLSKKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQSAFIDPHSYLA 880
            LP Y N  +VN  K AE ++KRKLLWSKK +    + +A  W  +S++A +GD   AAKFRKLMGIHD+P    S  D  K+  EQA LF RL+ +Y+ +R+ THT RGVGLG++ +  +D  +Y A
Sbjct:  185 LPKYYNPGSVNAVKLAEQQEKRKLLWSKKKEDNAQDTKASAWTTTSLVAGKGDSAAAAKFRKLMGIHDDPGQDGSQDDAQKRAEEQAELFRRLEYEYEQSRALTHTQRGVGLGFSSATHVDYGAYSA 311
[Close]
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. TrEMBL
Match: A0A4S2M8P3 (SMAP domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002841 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 7.784e-19
Identity = 61/127 (48.03%), Postives = 82/127 (64.57%), Query Frame = 2 
Query:  509 LPCYLNSAAVNPTKYAEIEQXXXXXXXXXXXXXXXN--IEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQ-STIDLSKKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQSAFIDPHSYLA 880
            LP Y N  +VN  K AE ++KRKLLWSKK +    +    AW  +S++A +GD   AAKFRKLMGIHD+P    S  D  K+  EQA LF RL+ +Y+ +R+ THT RGVGLG++ +  +D  +Y A
Sbjct:  266 LPKYYNPGSVNAVKLAEQQEKRKLLWSKKKEDNAPDNKASAWTTTSLVAGKGDSAAAAKFRKLMGIHDDPGQDGSQDDAQKRAEEQAELFRRLEYEYEQSRALTHTQRGVGLGFSSAPHVDYGAYSA 392
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. TrEMBL
Match: G7YGE6 (Arginine/serine-rich coiled-coil protein 2 OS=Clonorchis sinensis OX=79923 GN=CLF_107243 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 1.282e-18
Identity = 61/127 (48.03%), Postives = 82/127 (64.57%), Query Frame = 2 
Query:  509 LPCYLNSAAVNPTKYAEIEQXXXXXXXXXXXXXXXN--IEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQ-STIDLSKKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQSAFIDPHSYLA 880
            LP Y N  +VN  K AE ++KRKLLWSKK +    +    AW  +S++A +GD   AAKFRKLMGIHD+P    S  D  K+  EQA LF RL+ +Y+ +R+ THT RGVGLG++ +  +D  +Y A
Sbjct:  245 LPKYYNPGSVNAVKLAEQQEKRKLLWSKKKEDNAPDNKASAWTTTSLVAGKGDSAAAAKFRKLMGIHDDPGQDGSQDDAQKRAEEQAELFRRLEYEYEQSRALTHTQRGVGLGFSSATHVDYGAYSA 371
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. TrEMBL
Match: A0A5J4NNT6 (SMAP domain-containing protein OS=Paragonimus westermani OX=34504 GN=DEA37_0015239 PE=4 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 5.097e-18
Identity = 62/140 (44.29%), Postives = 85/140 (60.71%), Query Frame = 2 
Query:  509 LPCYLNSAAVNPTKYAEIEQXXXXXXXXXXXXXX----XNIEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQS--------TIDLSKKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQSAFIDPHSYLAGRRD 892
            LP Y N  +VNP K AE ++KRKLLWSKK D            W  +S+IA +GD   AAKFRKLMGIHD+ + +           D  K+  EQA LF RL+ +Y+ +R+ THT RGVGLG++ +A +D  +Y A +++
Sbjct:  110 LPKYYNPTSVNPLKLAEQQEKRKLLWSKKKDDSGDPKDSKTSLWTTTSIIAGKGDTAAAAKFRKLMGIHDDTDLKPRDGRDTDIDTDAQKRAEEQAELFRRLELEYEQSRALTHTQRGVGLGFSSAAHVDYSAYSAMQQE 249
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Cavefish
Match: rsrc2 (arginine/serine-rich coiled-coil 2 [Source:NCBI gene;Acc:406566])

HSP 1 Score: 52.373 bits (124), Expect = 3.588e-7
Identity = 28/68 (41.18%), Postives = 37/68 (54.41%), Query Frame = 2 
Query:  656 GDDKKAAKFRKLMGI------HDEPNAQSTIDLSKKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGY 841
            G+  +  KFRKLMGI         P+A    +  K   +Q  +F  LD  Y+ ARS THT RG+GLG+
Sbjct:  313 GNKDQNVKFRKLMGIKGEEEEEGGPSAVLNDEGMKTLQKQEEMFRNLDVQYEMARSQTHTQRGMGLGF 380
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Medaka
Match: rsrc2 (arginine/serine-rich coiled-coil 2 [Source:NCBI gene;Acc:406566])

HSP 1 Score: 73.9442 bits (180), Expect = 3.611e-14
Identity = 52/123 (42.28%), Postives = 69/123 (56.10%), Query Frame = 2 
Query:  497 SGVKLPCYLNSAAVNPTKYAEIEQXXXXX-XXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGIH--DEPNAQSTIDLS--KKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQS 850
            +G+ +P Y N +AVNP K+AE E+KRK+L   KK+  K    E W   +     G+  +  KFRKLMGI   DE  A   I+    K   +Q  +F  LD  Y+ ARS THT RG+GLG+  S
Sbjct:  228 TGIAVPTYYNPSAVNPMKFAEQEKKRKMLWQGKKEGDKSQTAELWEKLNF----GNKDQNVKFRKLMGIKGDDEAEASKPINEEGLKTLQKQEEMFRNLDVQYEMARSQTHTQRGMGLGFTSS 346
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Planmine SMEST
Match: SMESG000023487.1 (SMESG000023487.1)

HSP 1 Score: 412.92 bits (1060), Expect = 8.001e-145
Identity = 285/288 (98.96%), Postives = 285/288 (98.96%), Query Frame = 2 
Query:   35 NLVKTDLKPVVFSKSPIINNSGSDTDSSVDASKNVKSHSELTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNKYKSSGYHXXXXXXXXXXXXXXXXXERSRSPRFDRSTKKSHREDRKKKYKDSSPRRANRHNDDTSKAADETSGVKLPCYLNSAAVNPTKYAEIEQXXXXXXXXXXXXXXXNIEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQSTIDLSKKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQSAFIDPHSYLAGRRDDN 898
            NLVKTDLKPVVFSKSPIINNSGSDTDSSVDASKNVKSHSELTRSKHRRSSSHKKSKHHKSKRSRSRDHHISKSRRSRSKDHSNKYKSSGYHKKPRDSSKSD RK FSK RSRSPRFDRSTKKSHREDRKKKYKDSSPRRANRHNDDTSKAADETSGVKLPCYLNSAAVNPTKYAEIEQKRKLLWSKKDDSKKLNIEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQSTIDLSKKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQSAFIDPHSYLAGRRDDN
Sbjct:    3 NLVKTDLKPVVFSKSPIINNSGSDTDSSVDASKNVKSHSELTRSKHRRSSSHKKSKHHKSKRSRSRDHHISKSRRSRSKDHSNKYKSSGYHKKPRDSSKSDIRKHFSKGRSRSPRFDRSTKKSHREDRKKKYKDSSPRRANRHNDDTSKAADETSGVKLPCYLNSAAVNPTKYAEIEQKRKLLWSKKDDSKKLNIEAWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQSTIDLSKKEHEQAVLFNRLDADYQSARSHTHTSRGVGLGYNQSAFIDPHSYLAGRRDDN 290
The following BLAST results are available for this feature:
BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
RSRC25.402e-1544.26arginine and serine rich coiled-coil 2 [Source:HGN... [more]
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ZC262.21.298e-638.46pep chromosome:WBcel235:III:8332223:8333572:1 gene... [more]
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rsrc23.423e-1441.67arginine/serine-rich coiled-coil 2 [Source:NCBI ge... [more]
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ccnj5.991e-1643.44cyclin J [Source:Xenbase;Acc:XB-GENE-485597][more]
ccnj6.273e-1643.44cyclin J [Source:Xenbase;Acc:XB-GENE-485597][more]
ccnj6.904e-1643.44cyclin J [Source:Xenbase;Acc:XB-GENE-485597][more]
ccnj8.438e-1643.44cyclin J [Source:Xenbase;Acc:XB-GENE-485597][more]
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rsrc21.733e-1544.26arginine/serine-rich coiled-coil 2 [Source:MGI Sym... [more]
Rsrc22.769e-1544.26arginine/serine-rich coiled-coil 2 [Source:MGI Sym... [more]
Rsrc23.225e-1544.26arginine/serine-rich coiled-coil 2 [Source:MGI Sym... [more]
Rsrc23.225e-1544.26arginine/serine-rich coiled-coil 2 [Source:MGI Sym... [more]
Rsrc23.541e-1544.26arginine/serine-rich coiled-coil 2 [Source:MGI Sym... [more]
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q7ZYR8|RSRC2_XENLA1.066e-1543.44Arginine/serine-rich coiled-coil protein 2 OS=Xeno... [more]
sp|Q5XHJ5|RSRC2_XENTR3.174e-1543.44Arginine/serine-rich coiled-coil protein 2 OS=Xeno... [more]
sp|A6QLS2|RSRC2_BOVIN2.210e-1444.26Arginine/serine-rich coiled-coil protein 2 OS=Bos ... [more]
sp|A2RTL5|RSRC2_MOUSE2.256e-1444.26Arginine/serine-rich coiled-coil protein 2 OS=Mus ... [more]
sp|Q5PQR4|RSRC2_RAT2.362e-1444.26Arginine/serine-rich coiled-coil protein 2 OS=Ratt... [more]
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S8WTM26.271e-2048.03SMAP domain-containing protein (Fragment) OS=Opist... [more]
A0A075A1D81.973e-1948.03SMAP domain-containing protein OS=Opisthorchis viv... [more]
A0A4S2M8P37.784e-1948.03SMAP domain-containing protein OS=Opisthorchis fel... [more]
G7YGE61.282e-1848.03Arginine/serine-rich coiled-coil protein 2 OS=Clon... [more]
A0A5J4NNT65.097e-1844.29SMAP domain-containing protein OS=Paragonimus west... [more]
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rsrc23.588e-741.18arginine/serine-rich coiled-coil 2 [Source:NCBI ge... [more]
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rsrc23.611e-1442.28arginine/serine-rich coiled-coil 2 [Source:NCBI ge... [more]
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BLAST of Arginine/serine-rich coiled-coil protein 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000023487.18.001e-14598.96SMESG000023487.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013337 ID=SMED30013337|Name=Arginine/serine-rich coiled-coil protein 2|organism=Schmidtea mediterranea sexual|type=transcript|length=1175bp
CTTGCATTATTAATCAACAAATATGGATAGAAATAATCTGGTTAAAACCG
ATTTGAAACCAGTAGTCTTTTCCAAATCTCCAATTATTAATAATTCGGGA
TCTGATACGGATTCGTCAGTTGATGCTTCCAAAAATGTAAAGAGTCATTC
CGAATTGACTCGCTCCAAGCACAGACGCAGCTCAAGTCATAAAAAGTCAA
AACATCATAAATCTAAAAGATCACGGTCTAGAGATCATCACATTTCCAAA
TCTAGAAGAAGTCGAAGTAAAGATCATAGTAATAAATATAAAAGTTCTGG
TTATCATAAGAAACCTCGTGATTCCTCGAAGTCTGATAAAAGAAAACAGT
TTAGCAAAGAGAGGTCCCGATCACCTAGATTTGATCGTTCAACTAAAAAA
TCTCATCGAGAAGATCGCAAGAAAAAATATAAAGATTCGTCTCCTAGAAG
AGCAAATAGGCATAATGATGACACTTCCAAGGCAGCTGATGAAACTAGCG
GAGTTAAGCTTCCATGCTATCTAAATTCTGCAGCCGTCAATCCCACTAAA
TATGCCGAAATCGAACAGAAACGGAAATTATTGTGGTCCAAAAAAGATGA
TTCCAAAAAACTGAATATCGAAGCCTGGAATGCTTCCTCGGTCATTGCCA
GTCGCGGTGACGACAAAAAGGCTGCAAAATTCCGGAAATTGATGGGAATT
CACGATGAGCCAAATGCACAAAGTACGATTGATCTCTCGAAAAAGGAGCA
CGAACAAGCCGTGCTGTTTAATCGGTTGGATGCTGATTACCAGTCGGCAC
GCAGCCACACGCACACGTCACGAGGGGTCGGACTCGGCTATAACCAGTCG
GCATTTATCGATCCACACTCGTATCTGGCCGGCCGGCGGGACGACAACTG
AATTCGGTGTGAGAGGAGATCTGTTATATAAATTTTGTTGAATATATTGT
GTTTTCTATTGATTTTTTGTGCAATTGTTGTTGATTAATGATTTTTGTTT
CTATTATTCAAATTGACACGAGAATATCACAGAAGTGACAAAATATCATC
TATCAAAAATATCTGGTCTCCTTCAGAAGCGGGTTAAAAATTACATGTAT
TACCGTGACTGTTATTATCTAATGTTAAATAAAAATTGTTGAAATTTGTG
ACGTGGATTCTTATGTGAAGACTAG
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protein sequence of SMED30013337-orf-1

>SMED30013337-orf-1 ID=SMED30013337-orf-1|Name=SMED30013337-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=293bp
MDRNNLVKTDLKPVVFSKSPIINNSGSDTDSSVDASKNVKSHSELTRSKH
RRSSSHKKSKHHKSKRSRSRDHHISKSRRSRSKDHSNKYKSSGYHKKPRD
SSKSDKRKQFSKERSRSPRFDRSTKKSHREDRKKKYKDSSPRRANRHNDD
TSKAADETSGVKLPCYLNSAAVNPTKYAEIEQKRKLLWSKKDDSKKLNIE
AWNASSVIASRGDDKKAAKFRKLMGIHDEPNAQSTIDLSKKEHEQAVLFN
RLDADYQSARSHTHTSRGVGLGYNQSAFIDPHSYLAGRRDDN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR028124SMAP_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR028124Small acidic protein-like domainPFAMPF15477SMAPcoord: 210..272
e-value: 2.8E-13
score: 50.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 116..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 44..85
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..163
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 86..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 18..34
NoneNo IPR availablePANTHERPTHR22426UNCHARACTERIZEDcoord: 154..273
NoneNo IPR availablePANTHERPTHR22426:SF2ARGININE/SERINE-RICH COILED-COIL PROTEIN 2coord: 154..273