SMED30013315

Overview
NameSMED30013315
Smed IDSMED30013315
Length (bp)615
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30013315 (SMED30013315) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30013315 (SMED30013315) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30013315 (SMED30013315) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30013315 (SMED30013315) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30013315

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 21

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30013315SMESG000022425.1 Contig4995newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30013315SMESG000022425.1 Contig4995uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30013315SMESG000032659.1 Contig4995newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30013315SMESG000032659.1 Contig4995uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
head regionSMED30013315SMESG000022425.1 SmedASXL_005498SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30013315SMESG000022425.1 dd_Smed_v4_3843_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30013315SMESG000022425.1 dd_Smed_v4_3843_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30013315SMESG000022425.1 dd_Smed_v4_3843_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30013315SMESG000022425.1 dd_Smed_v4_3843_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30013315SMESG000022425.1 dd_Smed_v4_3843_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30013315SMESG000022425.1 dd_Smed_v4_3843_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
non-ciliated neuronSMED30013315SMESG000022425.1 dd_Smed_v4_3843_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30013315SMESG000022425.1 dd_Smed_v4_3843_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013315SMESG000022425.1 Contig4995newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013315SMESG000022425.1 Contig4995uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013315SMESG000032659.1 Contig4995newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013315SMESG000032659.1 Contig4995uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30013315SMESG000022425.1 dd_Smed_v6_3843_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30013315SMESG000022425.1 dd_Smed_v4_3843_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ventral epidermisSMED30013315SMESG000022425.1 dd_Smed_v4_3843_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Category 4 cellSMED30013315SMESG000022425.1 dd_Smed_v4_3843_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30013315 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.006881:25669..26285 -3032
Homology
[Close]
BLAST of SMED30013315 vs. TrEMBL
Match: A0A1I8H4E9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 6.085e-7
Identity = 37/127 (29.13%), Postives = 70/127 (55.12%), Query Frame = 3 
Query:  156 YNNWVKDHPLTTNSVYGYFIQRNIEEKHAKEAYENQLKCQKQQEDKQLELHQ--PQIYTNIPNKNKPDFNH--SRRSNYYIAEPLPESITKGRFKRRFHSVPKSTEQEEKDIYLWNAVSGCTVRPTL 524
            YNNW+ + P T+N +YG++++   E +  ++  E Q    +Q+E+ QL   +  P +    P++++P F++  SR + Y++A PLP       + RR      ++    K + LWN +SG TV+ ++
Sbjct:  982 YNNWLSEKPHTSNQIYGFYMRE--EMRMRRQRLEQQ---DRQREEAQLRRARTAPNLLLPYPSQHRPPFSYTSSRPTTYFVAPPLPS-----LYDRR--QAKSASASRGKVLQLWNTLSGVTVQLSM 1096
[Close]
BLAST of SMED30013315 vs. TrEMBL
Match: A0A1I8J9B3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 6.170e-7
Identity = 37/127 (29.13%), Postives = 70/127 (55.12%), Query Frame = 3 
Query:  156 YNNWVKDHPLTTNSVYGYFIQRNIEEKHAKEAYENQLKCQKQQEDKQLELHQ--PQIYTNIPNKNKPDFNH--SRRSNYYIAEPLPESITKGRFKRRFHSVPKSTEQEEKDIYLWNAVSGCTVRPTL 524
            YNNW+ + P T+N +YG++++   E +  ++  E Q    +Q+E+ QL   +  P +    P++++P F++  SR + Y++A PLP       + RR      ++    K + LWN +SG TV+ ++
Sbjct:  680 YNNWLSEKPHTSNQIYGFYMRE--EMRMRRQRLEQQ---DRQREEAQLRRARTAPNLLLPYPSQHRPPFSYTSSRPTTYFVAPPLPS-----LYDRR--QAKSASASRGKVLQLWNTLSGVTVQLSM 794
[Close]
BLAST of SMED30013315 vs. TrEMBL
Match: A0A267ENT5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009196g1 PE=4 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 7.450e-6
Identity = 36/123 (29.27%), Postives = 67/123 (54.47%), Query Frame = 3 
Query:  156 YNNWVKDHPLTTNSVYGYFIQRNIEEKHAKEAYENQLKCQKQQEDKQLELHQ--PQIYTNIPNKNKPDFNH--SRRSNYYIAEPLPESITKGRFKRRFHSVPKSTEQEEKDIYLWNAVSGCTV 512
            YNNW+ + P T+N +YG++++   E +  ++  E Q    +Q+E+ QL   +  P +    P++++P F++  SR + Y++A PLP    + + K        ++    K + LWN +SG TV
Sbjct:    4 YNNWLSEKPHTSNQIYGFYMR--EEMRMRRQRLEQQ---DRQREEAQLRRARTAPNLLLPYPSQHRPPFSYTSSRPTTYFVAPPLPSLYDRRQAK-------SASASRGKVLQLWNTLSGVTV 114
[Close]
BLAST of SMED30013315 vs. Planmine SMEST
Match: SMESG000022425.1 (SMESG000022425.1)

HSP 1 Score: 273.863 bits (699), Expect = 2.520e-95
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 3 
Query:  135 MPTGPSSYNNWVKDHPLTTNSVYGYFIQRNIEEKHAKEAYENQLKCQKQQEDKQLELHQPQIYTNIPNKNKPDFNHSRRSNYYIAEPLPESITKGRFKRRFHSVPKSTEQEEKDIYLWNAVSGCTVRPTL 524
            MPTGPSSYNNWVKDHPLTTNSVYGYFIQRNIEEKHAKEAYENQLKCQKQQEDKQLELHQPQIYTNIPNKNKPDFNHSRRSNYYIAEPLPESITKGRFKRRFHSVPKSTEQEEKDIYLWNAVSGCTVRPTL
Sbjct:    1 MPTGPSSYNNWVKDHPLTTNSVYGYFIQRNIEEKHAKEAYENQLKCQKQQEDKQLELHQPQIYTNIPNKNKPDFNHSRRSNYYIAEPLPESITKGRFKRRFHSVPKSTEQEEKDIYLWNAVSGCTVRPTL 130
[Close]
BLAST of SMED30013315 vs. Planmine SMEST
Match: SMESG000016898.1 (SMESG000016898.1)

HSP 1 Score: 73.1738 bits (178), Expect = 8.521e-17
Identity = 42/122 (34.43%), Postives = 68/122 (55.74%), Query Frame = 3 
Query:  141 TGPSSYNNWVKDHPLTTNSVYGYFIQRNIEEKHAKEAYENQLKCQKQQEDKQLELHQPQIYTNIPNKNKPDFNHSRRSNYYIAEPLPESITKGRFKRRFHSVPKSTEQEEKDIYLWNAVSGC 506
            TGP  YNNW+K++P+TTN VYG+ I++ + E+  ++  +  L+ +K+Q     E   P +YT    KN   F HSR+  Y+IA+ +P+  +   F R  +    S   + K ++LWN    C
Sbjct:    2 TGPYYYNNWLKNNPVTTNGVYGFHIRKQLLEEKCEKERQKILELKKRQGLPVSEFKSPNLYTAGTEKN---FTHSRKQRYFIADLIPQHKS-SEFYRELY----SENDKHKAMFLWNTKKEC 115
The following BLAST results are available for this feature:
BLAST of SMED30013315 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30013315 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30013315 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30013315 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30013315 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30013315 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30013315 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30013315 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 3
Match NameE-valueIdentityDescription
A0A1I8H4E96.085e-729.13Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8J9B36.170e-729.13Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267ENT57.450e-629.27Uncharacterized protein OS=Macrostomum lignano OX=... [more]
back to top
BLAST of SMED30013315 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30013315 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30013315 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30013315 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30013315 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30013315 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000022425.12.520e-95100.00SMESG000022425.1[more]
SMESG000016898.18.521e-1734.43SMESG000016898.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013315 ID=SMED30013315|Name=SMED30013315|organism=Schmidtea mediterranea sexual|type=transcript|length=615bp
TGCTAACCATTGTTATTAAAGAATTGAAATGTGTTGTAGATTTTGATTGG
CTATTGCTATAGCAACTCTGGGTTACCAAGGAATTGAAAAACAATTTATT
CAAAGTTATCAGTGAAAAATAAATACTGAATAAAATGCCAACAGGCCCGT
CTTCTTACAATAATTGGGTGAAGGATCATCCGCTAACAACAAACAGTGTT
TATGGCTATTTCATTCAGAGAAATATCGAAGAAAAACATGCCAAAGAAGC
TTATGAAAATCAGTTGAAATGCCAAAAGCAACAGGAAGATAAACAACTGG
AGTTGCACCAGCCGCAGATCTATACGAATATTCCAAACAAAAATAAACCG
GACTTTAATCATTCGCGTAGATCCAATTATTATATAGCTGAACCTTTACC
CGAAAGTATCACGAAAGGACGTTTTAAAAGAAGATTTCATAGTGTCCCAA
AATCAACCGAGCAGGAAGAAAAGGATATTTACTTGTGGAATGCCGTTTCC
GGTTGCACAGTTCGCCCTACATTGTAGATTTTTAGACATGTTTATTGTAC
TTTTCTGTTGTCTCGAATTTGAAACTTTTAAACATTATAATTCTTTATCA
CAGACTTATTTTCCG
back to top

protein sequence of SMED30013315-orf-1

>SMED30013315-orf-1 ID=SMED30013315-orf-1|Name=SMED30013315-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=131bp
MPTGPSSYNNWVKDHPLTTNSVYGYFIQRNIEEKHAKEAYENQLKCQKQQ
EDKQLELHQPQIYTNIPNKNKPDFNHSRRSNYYIAEPLPESITKGRFKRR
FHSVPKSTEQEEKDIYLWNAVSGCTVRPTL*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000018Category 4 cell
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000096ventral epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 30..57