Calpain
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30013312 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 16Note: Hover over icons to view figure legend
Homology
BLAST of Calpain vs. Ensembl Human
Match: CAPN9 (calpain 9 [Source:HGNC Symbol;Acc:HGNC:1486]) HSP 1 Score: 523.087 bits (1346), Expect = 1.486e-174 Identity = 294/683 (43.05%), Postives = 403/683 (59.00%), Query Frame = 2
Query: 383 ASKVQAKHTPKKKEDVGQQTSYFDLLEQ-LKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAIL-VKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNA 2407
A QA PK F+ + Q G L+ED DFPA SS+F S+ + FVWKRP +I KNP+ G +R D+ QG LGDCWLLA +ASL+L + V+P DQ F Y G F F+FW+ +W++VV+DDRLPT+ +LVF+HSAD+NEFWSALLEKAYAK+ G YE+L GGS IEAMEDFTGG+ ET + KE P Y + K K+ +L+GC I++ A+ E GLI GHAYS+T + +V G +EL+R+RNPWG + EW G WSDSSPEW ++ +E++RLC +DGEFWM F DF +F K+EIC+L P++L ED +WE+ + GSW + AGGCRNF+D+F TNPQ + + + ++ + + ++ LMQKD R+ + G L IGY IY+ D + LNK FF++ + A+S FIN REV R KLP G Y++IPSTF P++EA+F LRIFSEK T +D G +I DQ EE LF +AG + A +L +LNA+L K FK L C+++++L D GK+EF +F W +K W F FD D +G +E R AL G +S+ L +++ RY D E+ + F+DF+ ++RL NA
Sbjct: 7 APGPQAHPVPKDARITHSSGQSFEQMRQECLQRGTLFEDADFPASNSSLFYSE--RPQIPFVWKRPGEIVKNPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVIPQDQSFGPGYAGIFHFQFWQHSEWLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAETFQT--KEAPENFYEILEKALKRGSLLGCFIDTRSAAESEARTPFGLIKGHAYSVTGIDQVS-FRGQRIELIRIRNPWG-QVEWNGSWSDSSPEWRSVGPAEQKRLCHTALDDGEFWMAFKDFKAHFDKVEICNLTPDALEED--AIHKWEVTVHQGSWVRGSTAGGCRNFLDTFWTNPQIK--LSLTEKDEGQEECSFLVALMQKDRRKLKRFGANVLTIGYAIYECPDKD-EHLNKDFFRYHASRARSKTFINLREVSDRFKLPPGEYILIPSTFEPHQEADFCLRIFSEKKAITRDMD---GNVDI-DLPEPPKPTPPDQETEEEQ------------RFRALFEQVAGEDMEVTAEELEYVLNAVLQKKKDIKFKKLSLISCKNIISLMDTSGNGKLEFDEFKVFWDKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSHLLQLIVLRYAD-EELQLDFDDFLNCLVRLENA 661
BLAST of Calpain vs. Ensembl Human
Match: CAPN3 (calpain 3 [Source:HGNC Symbol;Acc:HGNC:1480]) HSP 1 Score: 504.982 bits (1299), Expect = 5.979e-167 Identity = 275/682 (40.32%), Postives = 415/682 (60.85%), Query Frame = 2
Query: 485 LWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXN--TGTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCG---LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAIL--VKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
L+ DP+FP D +S+F S+ K +FVWKRP +IC+NP+ DG +R D+ QG LGDCW LA +A L+L + + V+P DQ F +NY G F F+FWR+G+WV+VV+DD LPTY +LVF S NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E ++ ++ P +Y M K ++ +LMGC+I++ + E MA GL+ GHAYS+T + +V G V+LVR+RNPWG + EW G WSD +W + K EK RL DGEFWM+++DF +FTK+EIC+L ++L D + W + + +G W + +AGGCRNF D+F TNPQ+R+++++ DD+ ++ + ++ LMQK+ R+ R LG IG+ IY++ G L K FF ++ + A+S +IN REV R +LP YVI+PST+ P++E F+LR+FSEK N ++ ++ + + D+PV + +++ +++ +F +AG I A +L+ +LN ++ KD+K GF LE CRS++AL D D +GK+ ++F +W IK W+ FK +D D +G + +E RNA+ + G ++N L+ ++ RY D I F+ FI +RL F +D +G E+L+ +Y
Sbjct: 74 LYVDPEFPPDETSLFYSQ--KFPIQFVWKRPPEICENPRFIIDGANRTDICQGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVAEFFEI--RDAPSDMYKIMKKAIERGSLMGCSIDTIIPVQYETRMACGLVRGHAYSVTGLDEVP-FKGEKVKLVRLRNPWG-QVEWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLTADALQSD--KLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGASLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVE---------------NTISVDRPVPQPG--SSDQESEEQQQFRNIFKQIAGDDMEICADELKKVLNTVVNKHKDLKT-HGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMN-IDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMY 728
BLAST of Calpain vs. Ensembl Human
Match: CAPN3 (calpain 3 [Source:HGNC Symbol;Acc:HGNC:1480]) HSP 1 Score: 501.13 bits (1289), Expect = 7.213e-165 Identity = 279/709 (39.35%), Postives = 422/709 (59.52%), Query Frame = 2
Query: 485 LWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNT--GTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCG---LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLD--------IKNGKTNIAKHLRDTDDVDSDQPVSEESXX-------------------XXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAIL--VKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
L+ DP+FP D +S+F S+ K +FVWKRP +IC+NP+ DG +R D+ QG LGDCW LA +A L+L + + V+P DQ F +NY G F F+FWR+G+WV+VV+DD LPTY +LVF S NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E ++ ++ P +Y M K ++ +LMGC+I++ + E MA GL+ GHAYS+T + +V G V+LVR+RNPWG + EW G WSD +W + K EK RL DGEFWM+++DF +FTK+EIC+L ++L D + W + + +G W + +AGGCRNF D+F TNPQ+R+++++ DD+ ++ + ++ LMQK+ R+ R LG IG+ IY++ G L K FF ++ + A+S +IN REV R +LP YVI+PST+ P++E F+LR+FSEK N ++ ++ +K KT + D + + + V +ES+ +++ +++ +F +AG I A +L+ +LN ++ KD+K GF LE CRS++AL D D +GK+ ++F +W IK W+ FK +D D +G + +E RNA+ + G ++N L+ ++ RY D I F+ FI +RL F +D +G E+L+ +Y
Sbjct: 74 LYVDPEFPPDETSLFYSQ--KFPIQFVWKRPPEICENPRFIIDGANRTDICQGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVAEFFEI--RDAPSDMYKIMKKAIERGSLMGCSIDTIIPVQYETRMACGLVRGHAYSVTGLDEVP-FKGEKVKLVRLRNPWG-QVEWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLTADALQSD--KLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGASLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVKKKKTKPIIFVSDRANSNKELGVDQESEEGKGKTSPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQIAGDDMEICADELKKVLNTVVNKHKDLKT-HGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMN-IDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMY 772
BLAST of Calpain vs. Ensembl Human
Match: CAPN2 (calpain 2 [Source:HGNC Symbol;Acc:HGNC:1479]) HSP 1 Score: 481.485 bits (1238), Expect = 3.234e-158 Identity = 270/672 (40.18%), Postives = 412/672 (61.31%), Query Frame = 2
Query: 476 TGKLWEDPDFPADTSSVF---VSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIE---SAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQF--RXXXXXXXXXXXXNTGTVIIGLMQKD-MRQRNLG--FLVIGYVIYKLKDPNCGL----LNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVK--DIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETM-ELQPED 2437
G L++DP FPA S++ + S R WKRP +IC +PQ G +R D+ QGALGDCWLLA +ASL+L E I VVP +Q F +NY G F F+FW++G+WVEVVVDDRLPT G+L+F+HSA+ +EFWSALLEKAYAK+ G YE+LSGG+ E EDFTGG+ E +L K+ P L+ + K ++ +L+GC+I+ +A E + L+ GHAYS+T ++V +++G++ +L+R+RNPWG E EW G+W+D+ P W TI E+ERL DGEFWM+F DF ++++++EIC+L P++L D ++W++ DG+W + AGGCRN+ ++F NPQ+ ++E D D+ED E+ T ++GL+QK RQR +G IG+ IY++ + G L+K+FF + +S FIN REV R KLP G Y+++PSTF PN++ +F +R+FSEK +D + N+ + DD+D +LF +AG I+A +L+ IL +L K DIK+ GF +E C+ +V + D D +GK+ K+F +W I+ ++ +++ D D +G + +E R AL G + L +V+ R+ D ++ +I F++F+ ++RL F+ +L PE+
Sbjct: 42 AGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYEL--KKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEV-ESNGSLQKLIRIRNPWG-EVEWTGRWNDNCPSWNTIDPEERERLTRRH-EDGEFWMSFSDFLRHYSRLEICNLTPDTLTSDTY--KKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELSGQTNIHLSKNFFLTNRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKDGDFCIRVFSEKKADYQAVD-DEIEANLEEFDISEDDIDDG--------------------FRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKS-DGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARFAD-DQLIIDFDNFVRCLVRLETLFKIFKQLDPEN 683
BLAST of Calpain vs. Ensembl Human
Match: CAPN3 (calpain 3 [Source:HGNC Symbol;Acc:HGNC:1480]) HSP 1 Score: 482.256 bits (1240), Expect = 6.384e-157 Identity = 279/757 (36.86%), Postives = 421/757 (55.61%), Query Frame = 2
Query: 485 LWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESA---------------------------------------------------KIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNT--GTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCG---LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLD--------IKNGKTNIAKHLRDTDDVDSDQPVSEESXX-------------------XXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAIL--VKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
L+ DP+FP D +S+F S+ K +FVWKRP +IC+NP+ DG +R D+ QG LGDCW LA +A L+L + + V+P DQ F +NY G F F+FWR+G+WV+VV+DD LPTY +LVF S NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E ++ ++ P +Y M K ++ +LMGC+I+ + E MA GL+ GHAYS+T + +V G V+LVR+RNPWG + EW G WSD +W + K EK RL DGEFWM+++DF +FTK+EIC+L ++L D + W + + +G W + +AGGCRNF D+F TNPQ+R+++++ DD+ ++ + ++ LMQK+ R+ R LG IG+ IY++ G L K FF ++ + A+S +IN REV R +LP YVI+PST+ P++E F+LR+FSEK N ++ ++ +K KT + D + + + V +ES+ +++ +++ +F +AG I A +L+ +LN ++ KD+K GF LE CRS++AL D D +GK+ ++F +W IK W+ FK +D D +G + +E RNA+ + G ++N L+ ++ RY D I F+ FI +RL F +D +G E+L+ +Y
Sbjct: 74 LYVDPEFPPDETSLFYSQ--KFPIQFVWKRPPEICENPRFIIDGANRTDICQGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVAEFFEI--RDAPSDMYKIMKKAIERGSLMGCSIDDGTNMTYGTSPSGLNMGELIARMVRNMDNSLLQDSDLDPRGSDERPTRTIIPVQYETRMACGLVRGHAYSVTGLDEVP-FKGEKVKLVRLRNPWG-QVEWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLTADALQSD--KLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGASLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVKKKKTKPIIFVSDRANSNKELGVDQESEEGKGKTSPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQIAGDDMEICADELKKVLNTVVNKHKDLKT-HGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMN-IDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMY 820
BLAST of Calpain vs. Ensembl Celegans
Match: clp-1 (Calpain clp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34308]) HSP 1 Score: 439.499 bits (1129), Expect = 5.827e-142 Identity = 231/477 (48.43%), Postives = 322/477 (67.51%), Query Frame = 2
Query: 446 YFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAK--IEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNE--DLSGKRR----WEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQK---DMRQRNLGFLVIGYVIYKLKDP--NCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFS 1837
+++L +Q + +L+EDP F A+ SS+F SK E W RP +I + PQL +G SRFDV QG LGDCWLLA A+L+L + +F VVP DQ F +NY G F F+FW++GKWV+VV+DDRLPT G+L++MHSA NNEFWSALLEKAYAK+ G YE+L GG+ EA+ED TGGL E I L K P L M++ + +L GC+IE+ E M+NGL+ GHAYSIT + V +G ++R+RNPWGNE+EW G WSD+S EW ++ S K+ + F +DGEFWM+FDDF +NF K+EIC+LGP+ ++E ++G + W DG+W +N AGGCRN+I++F NPQFRV++ D D +D + TVI ++QK +++Q L + IG+ +Y + + N G L+K FF + + +S FIN RE+ GR ++P G+YV++PSTF PNEEA FMLR+++
Sbjct: 257 FYELRDQCLESKRLFEDPQFLANDSSLFFSKRPPKRVE--WLRPGEITREPQLITEGHSRFDVIQGELGDCWLLAAAANLTLKDELFYRVVPPDQSFTENYAGIFHFQFWQYGKWVDVVIDDRLPTSNGELLYMHSASNNEFWSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFIDL--KNPPRNLMQMMMRGFEMGSLFGCSIEADPNVWEAKMSNGLVKGHAYSITGCRIVDGPNGQTC-ILRIRNPWGNEQEWNGPWSDNSREWRSVPDSVKQDMGLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSDPDDDDELCTVIFAVLQKYRRNLKQDGLDNVPIGFAVYDMNNAGNNRGRLSKQFFAANKSAMRSAAFINLREMTGRFRVPPGNYVVVPSTFEPNEEAEFMLRVYT 728
BLAST of Calpain vs. Ensembl Celegans
Match: clp-1 (Calpain clp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34308]) HSP 1 Score: 439.499 bits (1129), Expect = 5.827e-142 Identity = 231/477 (48.43%), Postives = 322/477 (67.51%), Query Frame = 2
Query: 446 YFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAK--IEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNE--DLSGKRR----WEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQK---DMRQRNLGFLVIGYVIYKLKDP--NCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFS 1837
+++L +Q + +L+EDP F A+ SS+F SK E W RP +I + PQL +G SRFDV QG LGDCWLLA A+L+L + +F VVP DQ F +NY G F F+FW++GKWV+VV+DDRLPT G+L++MHSA NNEFWSALLEKAYAK+ G YE+L GG+ EA+ED TGGL E I L K P L M++ + +L GC+IE+ E M+NGL+ GHAYSIT + V +G ++R+RNPWGNE+EW G WSD+S EW ++ S K+ + F +DGEFWM+FDDF +NF K+EIC+LGP+ ++E ++G + W DG+W +N AGGCRN+I++F NPQFRV++ D D +D + TVI ++QK +++Q L + IG+ +Y + + N G L+K FF + + +S FIN RE+ GR ++P G+YV++PSTF PNEEA FMLR+++
Sbjct: 257 FYELRDQCLESKRLFEDPQFLANDSSLFFSKRPPKRVE--WLRPGEITREPQLITEGHSRFDVIQGELGDCWLLAAAANLTLKDELFYRVVPPDQSFTENYAGIFHFQFWQYGKWVDVVIDDRLPTSNGELLYMHSASNNEFWSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFIDL--KNPPRNLMQMMMRGFEMGSLFGCSIEADPNVWEAKMSNGLVKGHAYSITGCRIVDGPNGQTC-ILRIRNPWGNEQEWNGPWSDNSREWRSVPDSVKQDMGLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSDPDDDDELCTVIFAVLQKYRRNLKQDGLDNVPIGFAVYDMNNAGNNRGRLSKQFFAANKSAMRSAAFINLREMTGRFRVPPGNYVVVPSTFEPNEEAEFMLRVYT 728
BLAST of Calpain vs. Ensembl Celegans
Match: clp-1 (Calpain clp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34308]) HSP 1 Score: 439.499 bits (1129), Expect = 5.827e-142 Identity = 231/477 (48.43%), Postives = 322/477 (67.51%), Query Frame = 2
Query: 446 YFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAK--IEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNE--DLSGKRR----WEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQK---DMRQRNLGFLVIGYVIYKLKDP--NCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFS 1837
+++L +Q + +L+EDP F A+ SS+F SK E W RP +I + PQL +G SRFDV QG LGDCWLLA A+L+L + +F VVP DQ F +NY G F F+FW++GKWV+VV+DDRLPT G+L++MHSA NNEFWSALLEKAYAK+ G YE+L GG+ EA+ED TGGL E I L K P L M++ + +L GC+IE+ E M+NGL+ GHAYSIT + V +G ++R+RNPWGNE+EW G WSD+S EW ++ S K+ + F +DGEFWM+FDDF +NF K+EIC+LGP+ ++E ++G + W DG+W +N AGGCRN+I++F NPQFRV++ D D +D + TVI ++QK +++Q L + IG+ +Y + + N G L+K FF + + +S FIN RE+ GR ++P G+YV++PSTF PNEEA FMLR+++
Sbjct: 257 FYELRDQCLESKRLFEDPQFLANDSSLFFSKRPPKRVE--WLRPGEITREPQLITEGHSRFDVIQGELGDCWLLAAAANLTLKDELFYRVVPPDQSFTENYAGIFHFQFWQYGKWVDVVIDDRLPTSNGELLYMHSASNNEFWSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFIDL--KNPPRNLMQMMMRGFEMGSLFGCSIEADPNVWEAKMSNGLVKGHAYSITGCRIVDGPNGQTC-ILRIRNPWGNEQEWNGPWSDNSREWRSVPDSVKQDMGLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSDPDDDDELCTVIFAVLQKYRRNLKQDGLDNVPIGFAVYDMNNAGNNRGRLSKQFFAANKSAMRSAAFINLREMTGRFRVPPGNYVVVPSTFEPNEEAEFMLRVYT 728
BLAST of Calpain vs. Ensembl Celegans
Match: clp-1 (Calpain clp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34308]) HSP 1 Score: 438.343 bits (1126), Expect = 2.266e-141 Identity = 231/475 (48.63%), Postives = 321/475 (67.58%), Query Frame = 2
Query: 446 YFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAK--IEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNE--DLSGKRR----WEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQK---DMRQRNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFS 1837
+++L +Q + +L+EDP F A+ SS+F SK E W RP +I + PQL +G SRFDV QG LGDCWLLA A+L+L + +F VVP DQ F +NY G F F+FW++GKWV+VV+DDRLPT G+L++MHSA NNEFWSALLEKAYAK+ G YE+L GG+ EA+ED TGGL E I L K P L M++ + +L GC+IE+ E M+NGL+ GHAYSIT + V +G ++R+RNPWGNE+EW G WSD+S EW ++ S K+ + F +DGEFWM+FDDF +NF K+EIC+LGP+ ++E ++G + W DG+W +N AGGCRN+I++F NPQFRV++ D D +D + TVI ++QK +++Q L + IG+ +Y + N G L+K FF + + +S FIN RE+ GR ++P G+YV++PSTF PNEEA FMLR+++
Sbjct: 282 FYELRDQCLESKRLFEDPQFLANDSSLFFSKRPPKRVE--WLRPGEITREPQLITEGHSRFDVIQGELGDCWLLAAAANLTLKDELFYRVVPPDQSFTENYAGIFHFQFWQYGKWVDVVIDDRLPTSNGELLYMHSASNNEFWSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFIDL--KNPPRNLMQMMMRGFEMGSLFGCSIEADPNVWEAKMSNGLVKGHAYSITGCRIVDGPNGQTC-ILRIRNPWGNEQEWNGPWSDNSREWRSVPDSVKQDMGLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSDPDDDDELCTVIFAVLQKYRRNLKQDGLDNVPIGFAVYDAGN-NRGRLSKQFFAANKSAMRSAAFINLREMTGRFRVPPGNYVVVPSTFEPNEEAEFMLRVYT 750
BLAST of Calpain vs. Ensembl Celegans
Match: clp-1 (Calpain clp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34308]) HSP 1 Score: 438.728 bits (1127), Expect = 3.161e-141 Identity = 230/475 (48.42%), Postives = 321/475 (67.58%), Query Frame = 2
Query: 446 YFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAK--IEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNE--DLSGKRR----WEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQK---DMRQRNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFS 1837
+++L +Q + +L+EDP F A+ SS+F SK + W RP +I + PQL +G SRFDV QG LGDCWLLA A+L+L + +F VVP DQ F +NY G F F+FW++GKWV+VV+DDRLPT G+L++MHSA NNEFWSALLEKAYAK+ G YE+L GG+ EA+ED TGGL E I L K P L M++ + +L GC+IE+ E M+NGL+ GHAYSIT + V +G ++R+RNPWGNE+EW G WSD+S EW ++ S K+ + F +DGEFWM+FDDF +NF K+EIC+LGP+ ++E ++G + W DG+W +N AGGCRN+I++F NPQFRV++ D D +D + TVI ++QK +++Q L + IG+ +Y + N G L+K FF + + +S FIN RE+ GR ++P G+YV++PSTF PNEEA FMLR+++
Sbjct: 303 FYELRDQCLESKRLFEDPQFLANDSSLFFSKRPP--KRVEWLRPGEITREPQLITEGHSRFDVIQGELGDCWLLAAAANLTLKDELFYRVVPPDQSFTENYAGIFHFQFWQYGKWVDVVIDDRLPTSNGELLYMHSASNNEFWSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFIDL--KNPPRNLMQMMMRGFEMGSLFGCSIEADPNVWEAKMSNGLVKGHAYSITGCRIVDGPNGQTC-ILRIRNPWGNEQEWNGPWSDNSREWRSVPDSVKQDMGLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSDPDDDDELCTVIFAVLQKYRRNLKQDGLDNVPIGFAVYDAGN-NRGRLSKQFFAANKSAMRSAAFINLREMTGRFRVPPGNYVVVPSTFEPNEEAEFMLRVYT 771
BLAST of Calpain vs. Ensembl Fly
Match: CalpB (gene:FBgn0025866 transcript:FBtr0076343) HSP 1 Score: 564.688 bits (1454), Expect = 0.000e+0 Identity = 305/695 (43.88%), Postives = 427/695 (61.44%), Query Frame = 2
Query: 455 LLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAK--IEGVMANGLISGHAYSITDVQKVKQTDGTV---VELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDL--SGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMR-QRNLGF--LVIGYVIYKLKDPNCGLLNK--SFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLD--IKNGKTN--IAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
L + G ++EDPDFPA +S+ S+ Y E W RP DI +PQ F +G SRFDVQQG LGDCWLLA A+L+ +F V+P DQDF +NY G F F+FW++GKWVEVV+DDRLPTY G+L++MHS + NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E + KE P L++ M+K A++ ++MGC++E +E GLI GHAYSIT V + + + ++R+RNPWGN+ EW G WSDSSPEW I + KE + NF DGEFWM+F DF +F ++EIC+L P+SL ED S +R+WEM + +G W + AGGCRNF+++F NPQ+ + + DPDDED + T I+ LMQK+ R +RN+G L IG+ IY L D + + + +FFK+ ++A+SP FIN REVC R KLP GHY+I+PSTF+PNEE F++R+FSE N + D + G+T+ IA L + D P L +LF+ +AG E ++ +L+ IL+ + + GF + RS+VA+ D D +G++ F++F + DI W+ FK +D G D F R AL + G ++N L +A+RY E G I F+DF+M I++ E + D+ F D++LE +Y
Sbjct: 249 LRDSCLANGTMFEDPDFPATNASLMYSRRPDRYYE--WLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDI--KEAPPNLFSIMMKAAERGSMMGCSLEPDPHVLEAETPQGLIRGHAYSITKVCLMDISTPNRQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGKCTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQGLNFFKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEENDDEVGFGETDDRIAPSLPPPTPKEEDDP--------------QRIALRRLFDSVAGSDEEVDWQELKRILDHSMRDVMVGSDGFSKDAVRSMVAMLDKDRSGRLGFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNALAHRYGSRE-GQIPFDDFLMCAIKVRTFIEMFRERDTDNSDTAFFNLDDWLERTIY 924
BLAST of Calpain vs. Ensembl Fly
Match: CalpB (gene:FBgn0025866 transcript:FBtr0304675) HSP 1 Score: 564.688 bits (1454), Expect = 0.000e+0 Identity = 305/695 (43.88%), Postives = 427/695 (61.44%), Query Frame = 2
Query: 455 LLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAK--IEGVMANGLISGHAYSITDVQKVKQTDGTV---VELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDL--SGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMR-QRNLGF--LVIGYVIYKLKDPNCGLLNK--SFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLD--IKNGKTN--IAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
L + G ++EDPDFPA +S+ S+ Y E W RP DI +PQ F +G SRFDVQQG LGDCWLLA A+L+ +F V+P DQDF +NY G F F+FW++GKWVEVV+DDRLPTY G+L++MHS + NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E + KE P L++ M+K A++ ++MGC++E +E GLI GHAYSIT V + + + ++R+RNPWGN+ EW G WSDSSPEW I + KE + NF DGEFWM+F DF +F ++EIC+L P+SL ED S +R+WEM + +G W + AGGCRNF+++F NPQ+ + + DPDDED + T I+ LMQK+ R +RN+G L IG+ IY L D + + + +FFK+ ++A+SP FIN REVC R KLP GHY+I+PSTF+PNEE F++R+FSE N + D + G+T+ IA L + D P L +LF+ +AG E ++ +L+ IL+ + + GF + RS+VA+ D D +G++ F++F + DI W+ FK +D G D F R AL + G ++N L +A+RY E G I F+DF+M I++ E + D+ F D++LE +Y
Sbjct: 249 LRDSCLANGTMFEDPDFPATNASLMYSRRPDRYYE--WLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDI--KEAPPNLFSIMMKAAERGSMMGCSLEPDPHVLEAETPQGLIRGHAYSITKVCLMDISTPNRQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGKCTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQGLNFFKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEENDDEVGFGETDDRIAPSLPPPTPKEEDDP--------------QRIALRRLFDSVAGSDEEVDWQELKRILDHSMRDVMVGSDGFSKDAVRSMVAMLDKDRSGRLGFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNALAHRYGSRE-GQIPFDDFLMCAIKVRTFIEMFRERDTDNSDTAFFNLDDWLERTIY 924
BLAST of Calpain vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0086530) HSP 1 Score: 487.649 bits (1254), Expect = 7.240e-163 Identity = 249/491 (50.71%), Postives = 329/491 (67.01%), Query Frame = 2
Query: 446 YFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAK--IEGVMANGLISGHAYSITDV---QKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDL--SGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMR-QRNLGF--LVIGYVIYKLKDPNCGLLNK----SFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKN 1876
Y +L +G L+EDP FPA S+ S+ R W RP +I +NPQ F +G SRFDVQQG LGDCWLLA A+L+ +F V+P +Q F++NY G F FRFW++GKWV+V++DDRLPTY G+L++MHS + NEFWSALLEKAYAK+ G YE+L GGS EAMEDFTGG+ E L KE P L+ + K A+++++MGC+IE E GLI GHAYSIT V V + ++R+RNPWGNE EW G WSDSSPEW I + +K + F DGEFWM+F DF +F ++EIC+L P+SL ED SGKR+WEM + +G W + AGGCRNF+D+F NPQ+ + +VDPD+ED E TVI+ LMQK+ R +RN+G L IG+ IY L D L N+ +FF++ +++ +SP FIN REVC R KLP GHY+I+PSTF+PNEE F++R+FSE N + + + N
Sbjct: 75 YETILNSCLASGSLFEDPLFPASNESLQFSRRPD--RHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDL--KEAPGNLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDIVTPNRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRE--LENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMECVQVDN 559
BLAST of Calpain vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0112869) HSP 1 Score: 485.723 bits (1249), Expect = 1.489e-158 Identity = 249/488 (51.02%), Postives = 327/488 (67.01%), Query Frame = 2
Query: 446 YFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAK--IEGVMANGLISGHAYSITDV---QKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDL--SGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMR-QRNLGF--LVIGYVIYKLKDPNCGLLNK----SFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLD 1867
Y +L +G L+EDP FPA S+ S+ R W RP +I +NPQ F +G SRFDVQQG LGDCWLLA A+L+ +F V+P +Q F++NY G F FRFW++GKWV+V++DDRLPTY G+L++MHS + NEFWSALLEKAYAK+ G YE+L GGS EAMEDFTGG+ E L KE P L+ + K A+++++MGC+IE E GLI GHAYSIT V V + ++R+RNPWGNE EW G WSDSSPEW I + +K + F DGEFWM+F DF +F ++EIC+L P+SL ED SGKR+WEM + +G W + AGGCRNF+D+F NPQ+ + +VDPD+ED E TVI+ LMQK+ R +RN+G L IG+ IY L D L N+ +FF++ +++ +SP FIN REVC R KLP GHY+I+PSTF+PNEE F++R+FSE N + D
Sbjct: 75 YETILNSCLASGSLFEDPLFPASNESLQFSRRPD--RHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDL--KEAPGNLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDIVTPNRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRE--LENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEEND 556 HSP 2 Score: 83.5741 bits (205), Expect = 5.129e-16 Identity = 41/131 (31.30%), Postives = 72/131 (54.96%), Query Frame = 2
Query: 2099 GFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
GF ++CRS+VA+ D D +GK+ F++F + +I WK FK +D + G F+ R AL + G ++N + V+ +RY G I F+DFIM +++ + + + + FT +E++E +Y
Sbjct: 698 GFSKDVCRSMVAMLDADKSGKLGFEEFETLLSEIAKWKAIFKVYDVENTGRVSGFQLREALNSAGYHLNNRVLNVLGHRY-GSRDGKIAFDDFIMCAVKIKTYIDIFKERDTEKNETATFTLEEWIERTIY 827
BLAST of Calpain vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0086529) HSP 1 Score: 485.723 bits (1249), Expect = 1.489e-158 Identity = 249/488 (51.02%), Postives = 327/488 (67.01%), Query Frame = 2
Query: 446 YFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAK--IEGVMANGLISGHAYSITDV---QKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDL--SGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMR-QRNLGF--LVIGYVIYKLKDPNCGLLNK----SFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLD 1867
Y +L +G L+EDP FPA S+ S+ R W RP +I +NPQ F +G SRFDVQQG LGDCWLLA A+L+ +F V+P +Q F++NY G F FRFW++GKWV+V++DDRLPTY G+L++MHS + NEFWSALLEKAYAK+ G YE+L GGS EAMEDFTGG+ E L KE P L+ + K A+++++MGC+IE E GLI GHAYSIT V V + ++R+RNPWGNE EW G WSDSSPEW I + +K + F DGEFWM+F DF +F ++EIC+L P+SL ED SGKR+WEM + +G W + AGGCRNF+D+F NPQ+ + +VDPD+ED E TVI+ LMQK+ R +RN+G L IG+ IY L D L N+ +FF++ +++ +SP FIN REVC R KLP GHY+I+PSTF+PNEE F++R+FSE N + D
Sbjct: 75 YETILNSCLASGSLFEDPLFPASNESLQFSRRPD--RHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDL--KEAPGNLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDIVTPNRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRE--LENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEEND 556 HSP 2 Score: 83.5741 bits (205), Expect = 5.129e-16 Identity = 41/131 (31.30%), Postives = 72/131 (54.96%), Query Frame = 2
Query: 2099 GFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
GF ++CRS+VA+ D D +GK+ F++F + +I WK FK +D + G F+ R AL + G ++N + V+ +RY G I F+DFIM +++ + + + + FT +E++E +Y
Sbjct: 698 GFSKDVCRSMVAMLDADKSGKLGFEEFETLLSEIAKWKAIFKVYDVENTGRVSGFQLREALNSAGYHLNNRVLNVLGHRY-GSRDGKIAFDDFIMCAVKIKTYIDIFKERDTEKNETATFTLEEWIERTIY 827
BLAST of Calpain vs. Ensembl Zebrafish
Match: capn9 (calpain 9 [Source:ZFIN;Acc:ZDB-GENE-010724-2]) HSP 1 Score: 524.242 bits (1349), Expect = 3.517e-175 Identity = 292/686 (42.57%), Postives = 423/686 (61.66%), Query Frame = 2
Query: 443 SYFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIE---SAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKD---MRQRNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAIL--VKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFL 2476
+Y L + GKL+EDPDFPA S+F S+ + F WKRP +ICK PQ G +R D+ QG LGDCWLLA +ASL+L E VVP DQ FD+ Y G F F+FW+ KW++VVVDDRLP KLVF+HSADNNEFWSALLEKAYAK+ G YE+L GGS +EAMEDFTGG+ E + K P L+ + K ++ +++GC+I+ SA+ E + GL+ GHAYS+T V++V G V+L+RVRNPWG + EW G WSD+S EW I SEK+RL N +DGEFWM F DF N+ K+EIC+L P++L++D+S ++W + + +GSW + AGGCRN+ID+F TNPQF+ + + +D + +VI+ LMQK+ +R+ L IG+ IY+ D + K FF+++ + A+S +IN REV R +LP G+Y+++P+TF P++EA+F++R+FSEK K +++ N I L + + + ++E K L KLF +AGP VI+A +L+ +LN +L K++K F G L C S++ L D D +G MEF +F W +K W F +D D +G +E R+AL G+ ++N + +++ R+ D EK I F+D++ ++RL N F + +G +FL
Sbjct: 26 TYEQLRRECLEKGKLFEDPDFPAVDGSLFYSQ--RVPVNFEWKRPGEICKEPQFITGGATRTDICQGQLGDCWLLAAIASLTLKEETLNRVVPNDQTFDRGYAGIFHFQFWQHNKWMDVVVDDRLPCVRDKLVFLHSADNNEFWSALLEKAYAKLNGSYEALKGGSTMEAMEDFTGGVGEMYE--TKNGPNNLFLILKKAIERGSMLGCSIDITSSAESEAQTSTGLVKGHAYSVTGVEEVNYR-GAKVQLIRVRNPWG-QVEWNGPWSDNSREWTVIDSSEKKRLLQNSLDDGEFWMEFGDFKSNYDKVEICNLSPDALSDDVS--KQWAVNLFEGSWIRGSTAGGCRNYIDTFWTNPQFK--LHLENPDDGDKLCSVIVALMQKNRRRLRKEGLDLETIGFAIYEAPD-DQDHQGKDFFRYNPSKARSRTYINVREVSERFRLPPGNYLLVPTTFQPHKEADFLIRLFSEK--KAGAIEVGN---KIEADLPEPPKPNPPEEETDEE-----------KGLRKLFEQVAGPDNVISARELQHVLNNVLGRRKEVK-FDGLSLNTCISIINLMDVDGSGMMEFSEFKVFWDKLKKWIMLFLSYDVDRSGTMSSYELRSALNAAGMKLNNRILQLLGLRFAD-EKLEIDFDDYLTCIVRLENMFRIFQALDAQKKGEVSLNMHQFL 682
BLAST of Calpain vs. Ensembl Zebrafish
Match: capn3b (calpain 3b [Source:ZFIN;Acc:ZDB-GENE-041001-149]) HSP 1 Score: 507.679 bits (1306), Expect = 2.247e-168 Identity = 291/679 (42.86%), Postives = 413/679 (60.82%), Query Frame = 2
Query: 485 LWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGT--VVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMR-QRNLGFLV--IGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILV--KDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
L+EDP FPA SS+F S+ K + WKRP +ICKNPQ G +R D+ QG LGDCWLLA +A L+L + I K VVP DQ F +NY G F F+FWR+G+WV+VV+DDRLPTY KLVF S NNEFWSAL+EKAYAK+ G YE+L GG+ +EAMEDFTGG+ E ++ E P LYN M K K+ +LMGCAI++ A+GL+ GHAYS+T V++ K+ DG ++ LVRVR+PWG K +D W ++K+E+++ GEFWM F++F KNFTK+EIC+L P++L +D K W + + +G W K +AGGCRNF D++ TNPQFR +V + + E T TV++ LMQK R +R G + IG+ IY++K L+K FF ++ + A+S ++N REV R L G YVIIPSTF+P++E+ F+LR+FSE + + +D A+ + VD+ V ++ K E+ EK+ +F +AG INA +LR +LN ++ K++K +GF LE CRS++AL D D TG + ++F +W IK WK F FD D + FE RNAL G ++N L+ ++ RY + E + F+ +I ++RL F +D +G E+L+ +Y
Sbjct: 65 LFEDPLFPAQDSSLFYSE--KFPLKLEWKRPMEICKNPQFILGGANRTDICQGDLGDCWLLAAIACLTLNDVILKRVVPHDQSFTENYAGIFHFQFWRYGEWVDVVIDDRLPTYKNKLVFTRSGQNNEFWSALMEKAYAKLHGSYEALKGGNSLEAMEDFTGGVTEFYEITEA--PKELYNIMRKALKRGSLMGCAIDTLVPTAQRTKTASGLVRGHAYSVTGVEQGKRKDGKDLMIRLVRVRDPWGVAPPPACKSND----WVELAKTEQDKQRLQPGEQGEFWMCFEEFQKNFTKLEICNLTPDTLEDDQMFK--WNVTVHEGRWVKGCSAGGCRNFPDTYWTNPQFR--LVLLEADAKEKTCTVVVALMQKGRRVERCSGATLHNIGFAIYEMKGNQQQQLSKDFFLYNASTARSKSYVNIREVTERFCLKPGEYVIIPSTFDPHKESEFLLRVFSENRSTSKVID--------AEIEAEPMQVDN---VKKKIKPFEEEETEEEKQFRAIFQQIAGDDMEINANELRTVLNRVVAKHKELKT-EGFSLESCRSMIALLDTDGTGHLNLQEFKHLWNKIKQWKLVFTRFDTDKSSTISSFEMRNALTEAGFQLNNQLYDIICMRYAN-EHMELDFDSYISCLVRLEGMFRAFRAFDKDGDGLIKLNVFEWLQLTMY 718
BLAST of Calpain vs. Ensembl Zebrafish
Match: capn3a (calpain 3a, (p94) [Source:ZFIN;Acc:ZDB-GENE-040912-97]) HSP 1 Score: 507.679 bits (1306), Expect = 2.977e-168 Identity = 287/699 (41.06%), Postives = 425/699 (60.80%), Query Frame = 2
Query: 485 LWEDPDFPADTSSVF---VSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDG--TVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXN--TGTVIIGLMQKDMR-QRNLG--FLVIGYVIYKLKDPNCG---LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEV------------INACQLRDILNAILV--KDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
L+ED FPA S+ + S + VWKRPK+IC NPQ G SR D+ QG LGDCWLLA +A L+L + + V+P +Q F + Y G F F+FWR+G WV+VVVDDR+PT+ +LVF SA+ NEFWSALLEKAYAK+ G YE+L GG+ E MEDFTGG+ E ++ KE P LY M K ++ +LMGC+I+S A+ E A GL+ GHAYS+T V++ KQ+ + V LVR+RNPWG + EW G WSD+S EWE++SK+EKE+L A DGEFWM+F+DF KN+TKIEIC+L P++L +D K W + + +G W + +AGGCRN+ D+F TNPQ+R+ +++ DD+ ++ T ++ LMQK+ R +R LG L IG+ IY++ G + K FF +T A+S +IN REV R +L G YVIIPS++ P++E F+LR+FSEK N ++ ++ + +++D PV S + +++ +F +AG + ++A +L+D+LN ++ KDI + F E CRS++AL D D TG++ ++F ++W IK W+ FK +D D N +E RNA+ + G ++N L+ ++ RY + E + FE FI ++RL F + + +G+ + E+L+ +Y
Sbjct: 50 LFEDETFPATVDSLGYKELGHKSNKVKNIVWKRPKEICDNPQFIVGGASRTDICQGDLGDCWLLAAIACLTLNDKLLYRVIPQEQSFSEQYAGIFHFQFWRYGDWVDVVVDDRIPTFNNQLVFTKSAERNEFWSALLEKAYAKLHGSYEALKGGNTAEGMEDFTGGVTEFYEM--KEAPKELYKIMQKALERGSLMGCSIDSLVPARFETRTATGLVKGHAYSVTAVEECKQSQQKESRVRLVRLRNPWG-QVEWNGPWSDNSKEWESLSKAEKEKLQQQNAEDGEFWMSFEDFKKNYTKIEICNLTPDALEDDKLHK--WTVSVNEGRWVRGCSAGGCRNYPDTFWTNPQYRLRLLEEDDDPEDDEVACTFVVALMQKNRRKERKLGANLLTIGFSIYEVPKEMHGNKQHMQKEFFMSTTAKARSRAYINLREVTQRFRLSPGEYVIIPSSYEPHQEGEFILRVFSEKRNTSEEIE---------------NRIEADHPVP--SPASTGEESEEDQQFLSIFQHIAGDVSTGEAVSAWGSNMEVSANELKDVLNKVVSKHKDIHT-ESFSRECCRSMIALMDMDGTGRLNLQEFRQLWNKIKQWQEIFKRYDFDHNDTISSYEMRNAINDAGFRLNNQLYDIITMRYAN-ESMNVDFESFISCLVRLEGMFRAFQAFDQCGDGSIRLSVLEWLQLTMY 724
BLAST of Calpain vs. Ensembl Zebrafish
Match: capn3b (calpain 3b [Source:ZFIN;Acc:ZDB-GENE-041001-149]) HSP 1 Score: 501.901 bits (1291), Expect = 4.672e-166 Identity = 291/683 (42.61%), Postives = 413/683 (60.47%), Query Frame = 2
Query: 485 LWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGT--VVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMR-QRNLGFLV--IGYVIY----KLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILV--KDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
L+EDP FPA SS+F S+ K + WKRP +ICKNPQ G +R D+ QG LGDCWLLA +A L+L + I K VVP DQ F +NY G F F+FWR+G+WV+VV+DDRLPTY KLVF S NNEFWSAL+EKAYAK+ G YE+L GG+ +EAMEDFTGG+ E ++ E P LYN M K K+ +LMGCAI++ A+GL+ GHAYS+T V++ K+ DG ++ LVRVR+PWG K +D W ++K+E+++ GEFWM F++F KNFTK+EIC+L P++L +D K W + + +G W K +AGGCRNF D++ TNPQFR +V + + E T TV++ LMQK R +R G + IG+ IY ++K L+K FF ++ + A+S ++N REV R L G YVIIPSTF+P++E+ F+LR+FSE + + +D A+ + VD+ V ++ K E+ EK+ +F +AG INA +LR +LN ++ K++K +GF LE CRS++AL D D TG + ++F +W IK WK F FD D + FE RNAL G ++N L+ ++ RY + E + F+ +I ++RL F +D +G E+L+ +Y
Sbjct: 65 LFEDPLFPAQDSSLFYSE--KFPLKLEWKRPMEICKNPQFILGGANRTDICQGDLGDCWLLAAIACLTLNDVILKRVVPHDQSFTENYAGIFHFQFWRYGEWVDVVIDDRLPTYKNKLVFTRSGQNNEFWSALMEKAYAKLHGSYEALKGGNSLEAMEDFTGGVTEFYEITEA--PKELYNIMRKALKRGSLMGCAIDTLVPTAQRTKTASGLVRGHAYSVTGVEQGKRKDGKDLMIRLVRVRDPWGVAPPPACKSND----WVELAKTEQDKQRLQPGEQGEFWMCFEEFQKNFTKLEICNLTPDTLEDDQMFK--WNVTVHEGRWVKGCSAGGCRNFPDTYWTNPQFR--LVLLEADAKEKTCTVVVALMQKGRRVERCSGATLHNIGFAIYEVPKEMKGNQQQQLSKDFFLYNASTARSKSYVNIREVTERFCLKPGEYVIIPSTFDPHKESEFLLRVFSENRSTSKVID--------AEIEAEPMQVDN---VKKKIKPFEEEETEEEKQFRAIFQQIAGDDMEINANELRTVLNRVVAKHKELKT-EGFSLESCRSMIALLDTDGTGHLNLQEFKHLWNKIKQWKLVFTRFDTDKSSTISSFEMRNALTEAGFQLNNQLYDIICMRYAN-EHMELDFDSYISCLVRLEGMFRAFRAFDKDGDGLIKLNVFEWLQLTMY 722
BLAST of Calpain vs. Ensembl Zebrafish
Match: capn3b (calpain 3b [Source:ZFIN;Acc:ZDB-GENE-041001-149]) HSP 1 Score: 489.574 bits (1259), Expect = 2.139e-161 Identity = 280/668 (41.92%), Postives = 394/668 (58.98%), Query Frame = 2
Query: 485 LWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGT--VVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMR-QRNLGFLV--IGYVIYKLKDPNCG----LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILV--KDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEG 2446
L+EDP FPA SS+F S+ K + WKRP +ICKNPQ G +R D+ QG LGDCWLLA +A L+L + I K VVP DQ F +NY G F F+FWR+G+WV+VV+DDRLPTY KLVF S NNEFWSAL+EKAYAK+ G YE+L GG+ +EAMEDFTGG+ E ++ E P LYN M K K+ +LMGCAI++ A+GL+ GHAYS+T V++ K+ DG ++ LVRVR+PWG K +D W ++K+E+++ GEFWM F++F KNFTK+EIC+L P++L +D K W + + +G W K +AGGCRNF D++ TNPQFR +V + + E T TV++ LMQK R +R G + IG+ IY++ G L+K FF ++ + A+S ++N REV R L G YVIIPSTF+P++E+ F+LR++ N + + + +T K R +F +AG INA +LR +LN ++ K++K +GF LE CRS++AL D D TG + ++F +W IK WK F FD D + FE RNAL G ++N L+ ++ RY + E + F+ +I ++RL F +D +G
Sbjct: 74 LFEDPLFPAQDSSLFYSE--KFPLKLEWKRPMEICKNPQFILGGANRTDICQGDLGDCWLLAAIACLTLNDVILKRVVPHDQSFTENYAGIFHFQFWRYGEWVDVVIDDRLPTYKNKLVFTRSGQNNEFWSALMEKAYAKLHGSYEALKGGNSLEAMEDFTGGVTEFYEITEA--PKELYNIMRKALKRGSLMGCAIDTLVPTAQRTKTASGLVRGHAYSVTGVEQGKRKDGKDLMIRLVRVRDPWGVAPPPACKSND----WVELAKTEQDKQRLQPGEQGEFWMCFEEFQKNFTKLEICNLTPDTLEDDQMFK--WNVTVHEGRWVKGCSAGGCRNFPDTYWTNPQFR--LVLLEADAKEKTCTVVVALMQKGRRVERCSGATLHNIGFAIYEVCSNKHGNQQQQLSKDFFLYNASTARSKSYVNIREVTERFCLKPGEYVIIPSTFDPHKESEFLLRLY----NISHSQPFEEEETEEEKQFR------------------------------AIFQQIAGDDMEINANELRTVLNRVVAKHKELKT-EGFSLESCRSMIALLDTDGTGHLNLQEFKHLWNKIKQWKLVFTRFDTDKSSTISSFEMRNALTEAGFQLNNQLYDIICMRYAN-EHMELDFDSYISCLVRLEGMFRAFRAFDKDGDG 693
BLAST of Calpain vs. Ensembl Xenopus
Match: CAPN3 (calpain 3 [Source:NCBI gene;Acc:100497532]) HSP 1 Score: 518.079 bits (1333), Expect = 1.129e-171 Identity = 287/717 (40.03%), Postives = 432/717 (60.25%), Query Frame = 2
Query: 431 GQQTSYFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXN--TGTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCG---LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIK------------NGKTNIAKHLR-----DTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFK--GFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
G++ ++ +L ++ ++D +FP D SS+F S++ +F WKRP +IC+NPQ G +R D+ QG LGDCW LA +A L+L E + V+P DQ+F NY G F F+FWR+G WV+V++DD LPTY +LVF S+ NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E +L KE P +YN M K ++ +L+GC+I++ ++E MANGL+ GHAYS+T V++ K G ++LVR+RNPWG + EW G W D++ EW + KSEK RL DGEFWM++DDF KNFTK EIC+L P++L+ D + W + + +G W + +AGGCRN+ D++ TNPQ+R+++++ DD+ ++N + ++ LMQK+ R+ R G IG+ IY++ G L K FF ++ + AK +IN REVC R +LP YVIIPST+ P++E F+LR+FSEK N ++ ++ K + ++N K L D D D +++ + EK+ +F +AG I+A +L+ +LN ++ K K K GF LE CRS++AL D D G++ ++F +W+ IK W+ F FD D +G FE RNA+ G ++N L+ ++ RY + ++ + F+ FI +RL F +D +G E+L+ +Y
Sbjct: 47 GREKTFEELRKKCLQRNFHFQDREFPPDESSLFYSQSFP--IKFEWKRPPEICENPQFILGGANRTDICQGDLGDCWFLAAIACLTLNEKVLFRVIPPDQNFTDNYAGIFHFQFWRYGDWVDVIIDDYLPTYNNELVFTKSSQRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEL--KEAPKDMYNIMKKAFERGSLIGCSIDTLVPVQLETRMANGLVKGHAYSVTGVEETK-LKGKPIKLVRLRNPWG-QVEWNGAWGDNAKEWTMVDKSEKTRLQHQVQEDGEFWMSYDDFMKNFTKAEICNLTPDALDSD--SLQVWTVSVNEGRWVRGCSAGGCRNYPDTYWTNPQYRLKLLEEDDDPADNEVVCSFVVALMQKNRRKDRKAGANLFTIGFAIYEVPKEMHGNNQHLQKDFFLYNASKAKCKSYINMREVCQRFRLPPSEYVIIPSTYEPHQEGEFILRVFSEKRNISEEVENKIEAEKPIPIIFVSDRSNSNKELNVDGATDEDQKKMDAEKKDKTSASTDTETEEEKQFRNIFQQIAGDDMEISADELQSVLNNVVNKH-KTLKSSGFTLESCRSMIALMDTDGCGRLNLQEFYHLWQKIKQWQKIFLRFDSDQSGTISSFEMRNAINEAGFHLNNQLYDIITMRYAN-KRMDLDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMY 753
BLAST of Calpain vs. Ensembl Xenopus
Match: tgfbrap1 (transforming growth factor beta receptor associated protein 1 [Source:Xenbase;Acc:XB-GENE-1009020]) HSP 1 Score: 511.531 bits (1316), Expect = 4.952e-170 Identity = 290/688 (42.15%), Postives = 415/688 (60.32%), Query Frame = 2
Query: 410 PKKKEDVGQQT-----SYFDLLEQLKG----TGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIE---SAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKD---MRQRNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAIL--VKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETME 2422
P KE + QQT S EQLK G L+ED DFPA+ SS+F S+ K FVWKRPK++ +P+ G SR DV QG LGDCWLLA +ASL+L + I VVP DQ F Y G F F+FW+ +W++VV+DDRLPT+ +LVF+HSAD NEFWSALLEKAYAK+ G YE+L GGS +EAMEDFTGG+ ET ++ + P L++ + K K+ +++GC+I+ SA+ E GL+ GHAYSIT V G V+L+R+RNPWG + EW G WSD+S EW I +EK RL +DGEFWM F+DF ++F K+EIC+L P+SL++D K +WE+ + +GSW + AGGCRNF+++F +NPQ + + + +D + T I LMQK+ +R+ L IGY IY+ +L+K FFKF+ + A+S ++N RE+ R KLP G Y+ IP+TF P++EA+F LRIFSEK +D G+ NI L D +EE ++ LF ++G ++A +L+ +LNA+L K+IK FK L C ++++L D GK+ F +F W +K W F +D D +G +E R AL + G ++N + +++ RY D ++ I F+DF+ ++RL N++ +
Sbjct: 8 PSLKESIAQQTPRSIHSSGKTYEQLKNDCLRKGVLFEDVDFPANDSSLFYSE--KPSVPFVWKRPKEMVADPEFILGGASRTDVCQGDLGDCWLLAAIASLTLNDKILYRVVPPDQSFSTGYAGIFHFQFWQHNEWLDVVIDDRLPTFRNRLVFVHSADLNEFWSALLEKAYAKLNGSYEALKGGSTLEAMEDFTGGVTETYEI--RSAPANLFDILDKAIKKGSMLGCSIDITSSAETEAKTPQGLVKGHAYSITGAD-VVNFKGQKVKLIRIRNPWG-QVEWNGAWSDNSSEWNIIGAAEKNRLSQASLDDGEFWMDFEDFKRHFDKLEICNLTPDSLDDD--AKHKWEVTVHEGSWIRGSTAGGCRNFLETFWSNPQIK--LSLTEQDDGQKECTFIAALMQKNRRKLRKTGADLLTIGYTIYQCPGSE-SILSKDFFKFNPSKARSKTYVNLREISQRFKLPPGDYMFIPTTFEPHQEADFCLRIFSEKKADIKEMD---GEVNI--DLPDPPAPTPPHKETEEE-----------RQFRTLFEQISGQDLEVSAEELQYVLNAVLRRTKNIK-FKEMSLLSCNNIISLMDASGNGKLGFDEFKIFWDKLKTWIGIFMQYDLDKSGTMSSYELRLALKSAGFHLNNIVLQLIVLRYAD-DQFQIEFDDFLNCLVRLENSYRVFK 666
BLAST of Calpain vs. Ensembl Xenopus
Match: tgfbrap1 (transforming growth factor beta receptor associated protein 1 [Source:Xenbase;Acc:XB-GENE-1009020]) HSP 1 Score: 509.605 bits (1311), Expect = 2.583e-168 Identity = 290/688 (42.15%), Postives = 415/688 (60.32%), Query Frame = 2
Query: 410 PKKKEDVGQQT-----SYFDLLEQLKG----TGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIE---SAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKD---MRQRNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAIL--VKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETME 2422
P KE + QQT S EQLK G L+ED DFPA+ SS+F S+ K FVWKRPK++ +P+ G SR DV QG LGDCWLLA +ASL+L + I VVP DQ F Y G F F+FW+ +W++VV+DDRLPT+ +LVF+HSAD NEFWSALLEKAYAK+ G YE+L GGS +EAMEDFTGG+ ET ++ + P L++ + K K+ +++GC+I+ SA+ E GL+ GHAYSIT V G V+L+R+RNPWG + EW G WSD+S EW I +EK RL +DGEFWM F+DF ++F K+EIC+L P+SL++D K +WE+ + +GSW + AGGCRNF+++F +NPQ + + + +D + T I LMQK+ +R+ L IGY IY+ +L+K FFKF+ + A+S ++N RE+ R KLP G Y+ IP+TF P++EA+F LRIFSEK +D G+ NI L D +EE ++ LF ++G ++A +L+ +LNA+L K+IK FK L C ++++L D GK+ F +F W +K W F +D D +G +E R AL + G ++N + +++ RY D ++ I F+DF+ ++RL N++ +
Sbjct: 8 PSLKESIAQQTPRSIHSSGKTYEQLKNDCLRKGVLFEDVDFPANDSSLFYSE--KPSVPFVWKRPKEMVADPEFILGGASRTDVCQGDLGDCWLLAAIASLTLNDKILYRVVPPDQSFSTGYAGIFHFQFWQHNEWLDVVIDDRLPTFRNRLVFVHSADLNEFWSALLEKAYAKLNGSYEALKGGSTLEAMEDFTGGVTETYEI--RSAPANLFDILDKAIKKGSMLGCSIDITSSAETEAKTPQGLVKGHAYSITGAD-VVNFKGQKVKLIRIRNPWG-QVEWNGAWSDNSSEWNIIGAAEKNRLSQASLDDGEFWMDFEDFKRHFDKLEICNLTPDSLDDD--AKHKWEVTVHEGSWIRGSTAGGCRNFLETFWSNPQIK--LSLTEQDDGQKECTFIAALMQKNRRKLRKTGADLLTIGYTIYQCPGSE-SILSKDFFKFNPSKARSKTYVNLREISQRFKLPPGDYMFIPTTFEPHQEADFCLRIFSEKKADIKEMD---GEVNI--DLPDPPAPTPPHKETEEE-----------RQFRTLFEQISGQDLEVSAEELQYVLNAVLRRTKNIK-FKEMSLLSCNNIISLMDASGNGKLGFDEFKIFWDKLKTWIGIFMQYDLDKSGTMSSYELRLALKSAGFHLNNIVLQLIVLRYAD-DQFQIEFDDFLNCLVRLENSYRVFK 666
BLAST of Calpain vs. Ensembl Xenopus
Match: CAPN3 (calpain 3 [Source:NCBI gene;Acc:100497532]) HSP 1 Score: 501.516 bits (1290), Expect = 6.727e-165 Identity = 289/757 (38.18%), Postives = 426/757 (56.27%), Query Frame = 2
Query: 455 LLEQLKGTGKLWEDPDFPADTSSV----FVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES-----------------------------------------------AKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXN--TGTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCG---LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIK------------NGKTNIAKHLR-----DTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFK--GFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
L + G KL+EDP F S+ F K++K + +WKRPK+IC+NPQ G +R D+ QG LGDCW LA +A L+L E + V+P DQ+F NY G F F+FWR+G WV+V++DD LPTY +LVF S+ NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E +L KE P +YN M K ++ +L+GC+I+ ++E MANGL+ GHAYS+T V++ K G ++LVR+RNPWG + EW G W D++ EW + KSEK RL DGEFWM++DDF KNFTK EIC+L P++L+ D + W + + +G W + +AGGCRN+ D++ TNPQ+R+++++ DD+ ++N + ++ LMQK+ R+ R G IG+ IY++ G L K FF ++ + AK +IN REVC R +LP YVIIPST+ P++E F+LR+FSEK N ++ ++ K + ++N K L D D D +++ + EK+ +F +AG I+A +L+ +LN ++ K K K GF LE CRS++AL D D G++ ++F +W+ IK W+ F FD D +G FE RNA+ G ++N L+ ++ RY + ++ + F+ FI +RL F +D +G E+L+ +Y
Sbjct: 40 LKSEFVGKKKLFEDPIFLPAVQSLGQKEFAQKSTK-LKNIIWKRPKEICENPQFILGGANRTDICQGDLGDCWFLAAIACLTLNEKVLFRVIPPDQNFTDNYAGIFHFQFWRYGDWVDVIIDDYLPTYNNELVFTKSSQRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEL--KEAPKDMYNIMKKAFERGSLIGCSIDDKYGSSCGFAPPSNMEDAIMKMIESVGDTRPDTNGRGQPPKPVATLVPVQLETRMANGLVKGHAYSVTGVEETK-LKGKPIKLVRLRNPWG-QVEWNGAWGDNAKEWTMVDKSEKTRLQHQVQEDGEFWMSYDDFMKNFTKAEICNLTPDALDSD--SLQVWTVSVNEGRWVRGCSAGGCRNYPDTYWTNPQYRLKLLEEDDDPADNEVVCSFVVALMQKNRRKDRKAGANLFTIGFAIYEVPKEMHGNNQHLQKDFFLYNASKAKCKSYINMREVCQRFRLPPSEYVIIPSTYEPHQEGEFILRVFSEKRNISEEVENKIEAEKPIPIIFVSDRSNSNKELNVDGATDEDQKKMDAEKKDKTSASTDTETEEEKQFRNIFQQIAGDDMEISADELQSVLNNVVNKH-KTLKSSGFTLESCRSMIALMDTDGCGRLNLQEFYHLWQKIKQWQKIFLRFDSDQSGTISSFEMRNAINEAGFHLNNQLYDIITMRYAN-KRMDLDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMY 787
BLAST of Calpain vs. Ensembl Xenopus
Match: CAPN3 (calpain 3 [Source:NCBI gene;Acc:100497532]) HSP 1 Score: 499.975 bits (1286), Expect = 3.790e-164 Identity = 287/761 (37.71%), Postives = 431/761 (56.64%), Query Frame = 2
Query: 431 GQQTSYFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIE-----------------------------------------------SAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXN--TGTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCG---LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIK------------NGKTNIAKHLR-----DTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFK--GFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
G++ ++ +L ++ ++D +FP D SS+F S++ +F WKRP +IC+NPQ G +R D+ QG LGDCW LA +A L+L E + V+P DQ+F NY G F F+FWR+G WV+V++DD LPTY +LVF S+ NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E +L KE P +YN M K ++ +L+GC+I+ ++E MANGL+ GHAYS+T V++ K G ++LVR+RNPWG + EW G W D++ EW + KSEK RL DGEFWM++DDF KNFTK EIC+L P++L+ D + W + + +G W + +AGGCRN+ D++ TNPQ+R+++++ DD+ ++N + ++ LMQK+ R+ R G IG+ IY++ G L K FF ++ + AK +IN REVC R +LP YVIIPST+ P++E F+LR+FSEK N ++ ++ K + ++N K L D D D +++ + EK+ +F +AG I+A +L+ +LN ++ K K K GF LE CRS++AL D D G++ ++F +W+ IK W+ F FD D +G FE RNA+ G ++N L+ ++ RY + ++ + F+ FI +RL F +D +G E+L+ +Y
Sbjct: 47 GREKTFEELRKKCLQRNFHFQDREFPPDESSLFYSQSFP--IKFEWKRPPEICENPQFILGGANRTDICQGDLGDCWFLAAIACLTLNEKVLFRVIPPDQNFTDNYAGIFHFQFWRYGDWVDVIIDDYLPTYNNELVFTKSSQRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEL--KEAPKDMYNIMKKAFERGSLIGCSIDDKYGSSCGFAPPSNMEDAIMKMIESVGDTRPDTNGRGQPPKPVATLVPVQLETRMANGLVKGHAYSVTGVEETK-LKGKPIKLVRLRNPWG-QVEWNGAWGDNAKEWTMVDKSEKTRLQHQVQEDGEFWMSYDDFMKNFTKAEICNLTPDALDSD--SLQVWTVSVNEGRWVRGCSAGGCRNYPDTYWTNPQYRLKLLEEDDDPADNEVVCSFVVALMQKNRRKDRKAGANLFTIGFAIYEVPKEMHGNNQHLQKDFFLYNASKAKCKSYINMREVCQRFRLPPSEYVIIPSTYEPHQEGEFILRVFSEKRNISEEVENKIEAEKPIPIIFVSDRSNSNKELNVDGATDEDQKKMDAEKKDKTSASTDTETEEEKQFRNIFQQIAGDDMEISADELQSVLNNVVNKH-KTLKSSGFTLESCRSMIALMDTDGCGRLNLQEFYHLWQKIKQWQKIFLRFDSDQSGTISSFEMRNAINEAGFHLNNQLYDIITMRYAN-KRMDLDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMY 797
BLAST of Calpain vs. Ensembl Mouse
Match: Capn9 (calpain 9 [Source:MGI Symbol;Acc:MGI:1920897]) HSP 1 Score: 511.916 bits (1317), Expect = 2.577e-170 Identity = 285/694 (41.07%), Postives = 414/694 (59.65%), Query Frame = 2
Query: 389 KVQAKHTPKKKEDVGQQTSYFDLLEQ-LKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIK-NFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNA---FETMELQPED 2437
+ Q + P + F+ L Q +G L+ED DFPA S+F S+ + FVWKRP +I + P+ G +R D+ QG LGDCWLLA +ASL+L + VVP DQ F Y G F F+FW+ +W++VV+DDRLPT+ +LVF+HSAD+NEFWSALLEKAYAK+ G YE+L GGS IEAMEDFTGG+ E ++ +E P + + K K+ +L+GC+I++ ++ E + GLI GHAY++T + +V G ++L+RVRNPWG + EW G WSDSSPEW ++ E++RL +DGEFWM F DF +F K+EIC+L P++L + S RWE+ I GSW + AGGCRNF+D+F TNPQ + + + ++ + T + LMQKD R+ + G L IGY IY+ D + G L++ FF++ ++A+S FIN REV R +LP G Y++IPSTF P++EA+F LRIFSEK T LD +++D D P E+ E++ LF +AG ++A +L +LNA+L K FK L CR++++L D GK+EF++F W +K W F FD D +G +E R AL G + L +++ RY D E + F+D++ ++RL NA F+++ ++ +D
Sbjct: 9 RPQPQPVPGDARTIHSSGQSFEQLRQGCLQSGTLFEDADFPASNVSLFYSERPQ--VPFVWKRPGEIVEKPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALTRVVPQDQGFGSGYAGIFHFQFWQHSEWLDVVIDDRLPTFKDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAENFQI--REAPEDFFEILEKALKRGSLLGCSIDTLNASESEARTSLGLIKGHAYTVTGLDQVN-FHGQRIKLIRVRNPWG-QVEWNGPWSDSSPEWRSVDLEEQKRLGHTALDDGEFWMAFKDFKIHFDKVEICNLTPDALED--SALHRWEVTIHQGSWVRGSTAGGCRNFLDTFWTNPQIK--LSLTERDEGQEGCTFLAALMQKDRRRLKRFGANMLTIGYAIYQCPDKD-GHLSRDFFRYHASLARSKTFINLREVSERFQLPPGDYILIPSTFEPHQEADFCLRIFSEKRAVTRDLD---------------ENIDIDLP-ELPKPTPQEEETEEEQQFRALFQRVAGEDMEVSAEELEYVLNAVLQKKTALKFKRLSLLSCRNIISLMDTSGNGKLEFEEFRVFWDKLKHWMDLFLQFDVDKSGTMSSYELRTALKAAGFQLGGHLLQLIVLRYAD-EDLQLDFDDYLNCLVRLENASRVFQSLSVKNKD 674
BLAST of Calpain vs. Ensembl Mouse
Match: Capn3 (calpain 3 [Source:MGI Symbol;Acc:MGI:107437]) HSP 1 Score: 502.286 bits (1292), Expect = 4.487e-166 Identity = 275/682 (40.32%), Postives = 413/682 (60.56%), Query Frame = 2
Query: 485 LWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNT--GTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCG---LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAIL--VKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
L+ DP+FP D +S+F S+ K +FVWKRP +IC+NP+ G +R D+ QG LGDCW LA +A L+L E + V+P DQ F +NY G F F+FWR+G WV+VV+DD LPTY +LVF S NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E ++ K+ P +Y M K ++ +LMGC+I++ + E MA GL+ GHAYS+T +++ G V+LVR+RNPWG + EW G WSD +W + K EK RL DGEFWM++DDF +FTK+EIC+L ++L D + W + + +G W + +AGGCRNF D+F TNPQ+R+++++ DD+ ++ + ++ LMQK+ R+ R LG IG+ IY++ G L K FF ++ + A+S +IN REV R +LP YVI+PST+ P++E F+LR+FSEK N ++ + + + D+PV ++ +++ +F +AG I A +L+++LN ++ KD+K +GF LE CRS++AL D D +G++ ++F +WK IK W+ FK +D D +G + +E RNA+ + G +++ L+ ++ RY D I F+ FI +RL F +D +G E+L+ +Y
Sbjct: 74 LYLDPEFPPDETSLFYSQ--KFPIQFVWKRPPEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEI--KDAPSDMYKIMRKAIERGSLMGCSIDTIVPVQYETRMACGLVKGHAYSVTGLEEA-LFKGEKVKLVRLRNPWG-QVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTADALESD--KLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPEDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEAE---------------NTISVDRPVPRPG--HTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVNKHKDLKT-QGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMN-IDFDSFICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMY 728
BLAST of Calpain vs. Ensembl Mouse
Match: Capn3 (calpain 3 [Source:MGI Symbol;Acc:MGI:107437]) HSP 1 Score: 492.656 bits (1267), Expect = 1.500e-162 Identity = 273/694 (39.34%), Postives = 412/694 (59.37%), Query Frame = 2
Query: 455 LLEQLKGTGKLWEDPDFPADTSSVFVSKASKG--YREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXN--TGTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCG---LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAIL--VKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
L +Q L+ED FPA ++ + S+ + WKRPK+IC+NP+ G +R D+ QG LGDCW LA +A L+L E + V+P DQ F +NY G F F+FWR+G WV+VV+DD LPTY +LVF S NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E ++ K+ P +Y M K ++ +LMGC+I++ + E MA GL+ GHAYS+T +++ G V+LVR+RNPWG + EW G WSD +W + K EK RL DGEFWM++DDF +FTK+EIC+L ++L D + W + + +G W + +AGGCRNF D+F TNPQ+R+++++ DD+ ++ + ++ LMQK+ R+ R LG IG+ IY++ G L K FF ++ + A+S +IN REV R +LP YVI+PST+ P++E F+LR+FSEK N ++ + + + D+PV ++ +++ +F +AG I A +L+++LN ++ KD+K +GF LE CRS++AL D D +G++ ++F +WK IK W+ FK +D D +G + +E RNA+ + G +++ L+ ++ RY D I F+ FI +RL F +D +G E+L+ +Y
Sbjct: 40 LKQQCLAQKCLFEDRVFPAGVQALGSHELSQKAKMKAITWKRPKEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEI--KDAPSDMYKIMRKAIERGSLMGCSIDTIVPVQYETRMACGLVKGHAYSVTGLEEA-LFKGEKVKLVRLRNPWG-QVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTADALESD--KLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPEDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEAE---------------NTISVDRPVPRPG--HTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVNKHKDLKT-QGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMN-IDFDSFICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMY 708
BLAST of Calpain vs. Ensembl Mouse
Match: Capn3 (calpain 3 [Source:MGI Symbol;Acc:MGI:107437]) HSP 1 Score: 481.485 bits (1238), Expect = 6.792e-158 Identity = 273/722 (37.81%), Postives = 412/722 (57.06%), Query Frame = 2
Query: 455 LLEQLKGTGKLWEDPDFPADTSSVFVSKASKG--YREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXN--TGTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCG---LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILV------------------------------KDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
L +Q L+ED FPA ++ + S+ + WKRPK+IC+NP+ G +R D+ QG LGDCW LA +A L+L E + V+P DQ F +NY G F F+FWR+G WV+VV+DD LPTY +LVF S NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E ++ K+ P +Y M K ++ +LMGC+I++ + E MA GL+ GHAYS+T +++ G V+LVR+RNPWG + EW G WSD +W + K EK RL DGEFWM++DDF +FTK+EIC+L ++L D + W + + +G W + +AGGCRNF D+F TNPQ+R+++++ DD+ ++ + ++ LMQK+ R+ R LG IG+ IY++ G L K FF ++ + A+S +IN REV R +LP YVI+PST+ P++E F+LR+FSEK N ++ + + + D+PV ++ +++ +F +AG I A +L+++LN ++ KD+K +GF LE CRS++AL D D +G++ ++F +WK IK W+ FK +D D +G + +E RNA+ + G +++ L+ ++ RY D I F+ FI +RL F +D +G E+L+ +Y
Sbjct: 40 LKQQCLAQKCLFEDRVFPAGVQALGSHELSQKAKMKAITWKRPKEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEI--KDAPSDMYKIMRKAIERGSLMGCSIDTIVPVQYETRMACGLVKGHAYSVTGLEEA-LFKGEKVKLVRLRNPWG-QVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTADALESD--KLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPEDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEAE---------------NTISVDRPVPRPG--HTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVNKRERPTPDVGDGQGPQLSESSSSPFSIPPDKDLKT-QGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMN-IDFDSFICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMY 736
BLAST of Calpain vs. Ensembl Mouse
Match: Capn8 (calpain 8 [Source:MGI Symbol;Acc:MGI:2181366]) HSP 1 Score: 474.937 bits (1221), Expect = 7.795e-156 Identity = 277/696 (39.80%), Postives = 403/696 (57.90%), Query Frame = 2
Query: 455 LLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYRE---FVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIE---SAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTG----TVIIGLMQKDMRQRNL---GFLVIGYVIYK----LKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAK--HLRDTDDVDSD-QPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVK--DIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFL 2476
L +Q +G L++DP+FPA S++ G E +WKRP ++C NPQ G +R D++QG LGDCWLLA +ASL+L E + VVP DQ F KNY G F F+FW++G+WVEVV+DDRLPT G+L+F+HS + NEFWSALLEKAYAK+ G YE+L+GGS IE EDFTGG+ E L ++ P LY + K ++ +L+GC+I+ +A+ E L+ GHAYS+T V++V G +L+R+RNPWG E EW G WSDS+PEW I +K L A DGEFWM+F DF K F+++EIC+L P+SL+ + K W + + +G W + AGGC+N+ ++ TNPQF++ + + D++ E T TV++GLMQK+ R++ G L IGY +Y+ L++ L + FF+ S ++N REV R +LP G Y+++PSTF P ++ +F LR+FSEK K L+I + H RD D D +SEE A I+A QL+ +LN +L K D+K F GF++ CR +++L D D TG + +F +W I + +++ D G D E R AL G +++N + + +A RY + GV F+ F+ +IRL F+ L +D G + E+L
Sbjct: 35 LRKQCLNSGVLFKDPEFPACPSALGYRDLGPGSAETQGIIWKRPTELCSNPQFIVGGATRTDIRQGGLGDCWLLAAIASLTLNEKLLYRVVPRDQSFQKNYAGIFHFQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEEGNEFWSALLEKAYAKLNGSYEALAGGSTIEGFEDFTGGISEFYDL--RKPPGNLYYTIQKALRKGSLLGCSIDVSNAAEAEATTRQKLVKGHAYSVTGVEEV-DFRGLPEKLIRLRNPWG-EVEWTGAWSDSAPEWNYIDPQKKGEL-DKRAEDGEFWMSFSDFLKQFSRLEICNLSPDSLSSEEIHK--WNLVLFNGRWTRGSTAGGCQNYPATYWTNPQFKIHLDEVDEDQEEGTSEPCCTVLLGLMQKNRRRQRRIGQGMLSIGYAVYQIPKELENHTDEHLGRDFFQGRQPSTCSSTYMNLREVSSRVQLPPGQYLVVPSTFEPFKDGDFCLRVFSEK--KAQALEIGDAVAGDPHEPHPRDMDGEDEHFWSLSEE---------------------FADKDSEISAHQLKRVLNGLLSKRTDMK-FDGFNINTCREMISLLDGDGTGSLRPVEFKTLWLKICKYLEIYQEMDHSRAGTIDAHEMRTALKKAGFTLNNQVQQTIATRYACSKLGV-DFDGFVACMIRLEILFKLFRLLDKDQNGIVQLSLAEWL 698
BLAST of Calpain vs. UniProt/SwissProt
Match: sp|P27730|CAN_SCHMA (Calpain OS=Schistosoma mansoni OX=6183 PE=2 SV=1) HSP 1 Score: 598.971 bits (1543), Expect = 0.000e+0 Identity = 324/697 (46.48%), Postives = 442/697 (63.41%), Query Frame = 2
Query: 446 YFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFV-WKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFD-KNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGK--LVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESL--NEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQR-NLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNI------AKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGP-MEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLYM 2494
Y L+++LK LWEDPDFPA+ ++ +RE + WKRP +I N + FA G SRFD++QGALGDCWLLAVVAS+S Y +F VVP DQ+ Y+G RFRFWRFG WVEV++DDRLP G+ LVFMHS D EFWSALLEKAYAK+ G Y LSGGSQ EAMED TGG+C +++LN+KERP L +Q+ +A++ LMGC+I+S+ +E M NGLI HAYS+T V V G L+R+RNPWG+ EWKG W D SP+W IS+ EK+ + +F DGEFWM+++DF F+++E+CHLG ESL N++ GKRR + I G W +N+NAGGC N ++ T+PQFR+ V DPD +D +N +V+IGLMQ D+R++ F IG+++Y D LL+++ + IAKS FIN REV + ++P G YV+IPSTF+PN E NF+LR+FS+ LD N + A L DT +D DQ + ++ F + P INA +L ++LN ++DI NF+GF+ ELCRS+VA D + TG +E +FM +W K WK F D D +G F +EFR AL + G VSN L + NRY DP I+FEDF++ ++RL AFET+E P++ EG +F+A+++L +Y+
Sbjct: 86 YETLVKRLKTERTLWEDPDFPANDKAI---GNLPDFRERIEWKRPHEINPNAKFFAGGASRFDIEQGALGDCWLLAVVASISGYPQLFDQVVPKDQELKGPEYVGVVRFRFWRFGHWVEVLIDDRLPVRQGRNTLVFMHSNDPTEFWSALLEKAYAKLNGCYAHLSGGSQSEAMEDLTGGICLSLELNQKERPSDLIDQLKIYAQRCCLMGCSIDSSVMEQKMDNGLIGSHAYSLTGVYPVNYR-GRTQWLMRLRNPWGDSHEWKGAWCDGSPQWREISEQEKKNINLSFTADGEFWMSYEDFVTCFSRVEVCHLGLESLEYNQNFHGKRRLDEAIFSGQWQRNVNAGGCINNRTTYWTSPQFRITVEDPDPDDDDNKCSVLIGLMQTDIRKKVGADFQPIGFMVYNAPDDLNTLLSRAQLLTRSPIAKS-QFINTREVTAQFRVPPGSYVVIPSTFDPNIEVNFILRVFSQTSITEQELDEDNTNQGLPDDVIEALKLEDTL-LDEDQEIEQK------------------FLAIRDPKTNAINAVKLGELLNNSTLQDIPNFQGFNKELCRSMVASVDNNLTGHVELNEFMDLWIQAKGWKHIFIKHDVDQSGYFSAYEFREALNDAGYHVSNRLINAIINRYQDPGTDKISFEDFMLCMVRLKTAFETIEAHPKNIEGTSLFSAEDYLRFSVYI 758
BLAST of Calpain vs. UniProt/SwissProt
Match: sp|Q9VT65|CANB_DROME (Calpain-B OS=Drosophila melanogaster OX=7227 GN=CalpB PE=1 SV=2) HSP 1 Score: 564.688 bits (1454), Expect = 0.000e+0 Identity = 305/695 (43.88%), Postives = 427/695 (61.44%), Query Frame = 2
Query: 455 LLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAK--IEGVMANGLISGHAYSITDVQKVKQTDGTV---VELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDL--SGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMR-QRNLGF--LVIGYVIYKLKDPNCGLLNK--SFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLD--IKNGKTN--IAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
L + G ++EDPDFPA +S+ S+ Y E W RP DI +PQ F +G SRFDVQQG LGDCWLLA A+L+ +F V+P DQDF +NY G F F+FW++GKWVEVV+DDRLPTY G+L++MHS + NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E + KE P L++ M+K A++ ++MGC++E +E GLI GHAYSIT V + + + ++R+RNPWGN+ EW G WSDSSPEW I + KE + NF DGEFWM+F DF +F ++EIC+L P+SL ED S +R+WEM + +G W + AGGCRNF+++F NPQ+ + + DPDDED + T I+ LMQK+ R +RN+G L IG+ IY L D + + + +FFK+ ++A+SP FIN REVC R KLP GHY+I+PSTF+PNEE F++R+FSE N + D + G+T+ IA L + D P L +LF+ +AG E ++ +L+ IL+ + + GF + RS+VA+ D D +G++ F++F + DI W+ FK +D G D F R AL + G ++N L +A+RY E G I F+DF+M I++ E + D+ F D++LE +Y
Sbjct: 249 LRDSCLANGTMFEDPDFPATNASLMYSRRPDRYYE--WLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDI--KEAPPNLFSIMMKAAERGSMMGCSLEPDPHVLEAETPQGLIRGHAYSITKVCLMDISTPNRQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGKCTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQGLNFFKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEENDDEVGFGETDDRIAPSLPPPTPKEEDDP--------------QRIALRRLFDSVAGSDEEVDWQELKRILDHSMRDVMVGSDGFSKDAVRSMVAMLDKDRSGRLGFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNALAHRYGSRE-GQIPFDDFLMCAIKVRTFIEMFRERDTDNSDTAFFNLDDWLERTIY 924
BLAST of Calpain vs. UniProt/SwissProt
Match: sp|O35920|CAN9_RAT (Calpain-9 OS=Rattus norvegicus OX=10116 GN=Capn9 PE=2 SV=2) HSP 1 Score: 526.554 bits (1355), Expect = 4.447e-175 Identity = 290/681 (42.58%), Postives = 413/681 (60.65%), Query Frame = 2
Query: 389 KVQAKHTPKKKEDVGQQTSYFDLLEQ-LKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIK-NFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNA 2407
+ Q + P+ V F+ L Q +G L+ED DFPA SS+F S+ + FVWKRP +I +NP+ G +R D+ QG LGDCWLLA +ASL+L + VVP DQ F Y G F F+FW+ +W++VV+DDRLPT+ +LVF+HSAD+NEFWSALLEKAYAK+ G YE+L GGS IEAMEDFTGG+ E ++ +E P Y + K ++ +L+GC+I++ ++ E GLI GHAY++T + +V G ++L+RVRNPWG + EW G WSDSSPEW ++S E++RL +DGEFWM F+DF +F K+EIC+L P++L ++ K WE+ I GSW + AGGCRNF+D+F TNPQ + + + ++ + T + LMQKD R+ + G L IGY IY+ D + G LN+ FF++ ++A+S FIN REV GR +LP G Y++IPSTF P++EA+F LRIFSEK T LD NI D D + +P +E E++ LF +AG ++A +L +LNA+L K FK L CR++++L D GKMEF++F W ++ W F FD D +G +E R AL G + + L +++ RY D E + F+D++ ++RL NA
Sbjct: 9 RPQPQPVPRDARTVHSSGHSFEQLRQSCLQSGTLFEDADFPASNSSLFYSERPQ--VPFVWKRPGEIVENPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVVPQDQGFGSGYAGIFHFQFWQHSEWLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAENFQI--REAPEDFYEILEKALRRGSLLGCSIDTLNASESEARTPLGLIKGHAYTVTGLDQVN-FHGQRIKLIRVRNPWG-QVEWNGPWSDSSPEWRSMSLEEQKRLGHTALDDGEFWMAFEDFKTHFDKVEICNLTPDALEDNTLHK--WEVTIHQGSWVRGSTAGGCRNFLDTFWTNPQIK--LSLTERDEGQEGCTFLAALMQKDRRRLKRFGANMLTIGYAIYQCPDKD-GHLNRDFFRYHASLARSKTFINLREVSGRFQLPPGDYILIPSTFEPHQEADFCLRIFSEKKAVTQDLD-----ENI-----DIDLPELPKPTPQEE------ETEEERQFRALFRRIAGEDMEVSAEELEYVLNAVLQKKTALKFKRLSLLSCRNIISLMDTSGNGKMEFEEFRVFWDKLRYWMDLFLQFDVDKSGTMSSYELRTALKAAGFQLGSHLLQLIVLRYAD-ENLQLDFDDYLNCLVRLENA 661
BLAST of Calpain vs. UniProt/SwissProt
Match: sp|O14815|CAN9_HUMAN (Calpain-9 OS=Homo sapiens OX=9606 GN=CAPN9 PE=1 SV=1) HSP 1 Score: 523.087 bits (1346), Expect = 7.134e-174 Identity = 294/683 (43.05%), Postives = 403/683 (59.00%), Query Frame = 2
Query: 383 ASKVQAKHTPKKKEDVGQQTSYFDLLEQ-LKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAIL-VKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNA 2407
A QA PK F+ + Q G L+ED DFPA SS+F S+ + FVWKRP +I KNP+ G +R D+ QG LGDCWLLA +ASL+L + V+P DQ F Y G F F+FW+ +W++VV+DDRLPT+ +LVF+HSAD+NEFWSALLEKAYAK+ G YE+L GGS IEAMEDFTGG+ ET + KE P Y + K K+ +L+GC I++ A+ E GLI GHAYS+T + +V G +EL+R+RNPWG + EW G WSDSSPEW ++ +E++RLC +DGEFWM F DF +F K+EIC+L P++L ED +WE+ + GSW + AGGCRNF+D+F TNPQ + + + ++ + + ++ LMQKD R+ + G L IGY IY+ D + LNK FF++ + A+S FIN REV R KLP G Y++IPSTF P++EA+F LRIFSEK T +D G +I DQ EE LF +AG + A +L +LNA+L K FK L C+++++L D GK+EF +F W +K W F FD D +G +E R AL G +S+ L +++ RY D E+ + F+DF+ ++RL NA
Sbjct: 7 APGPQAHPVPKDARITHSSGQSFEQMRQECLQRGTLFEDADFPASNSSLFYSE--RPQIPFVWKRPGEIVKNPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVIPQDQSFGPGYAGIFHFQFWQHSEWLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAETFQT--KEAPENFYEILEKALKRGSLLGCFIDTRSAAESEARTPFGLIKGHAYSVTGIDQVS-FRGQRIELIRIRNPWG-QVEWNGSWSDSSPEWRSVGPAEQKRLCHTALDDGEFWMAFKDFKAHFDKVEICNLTPDALEED--AIHKWEVTVHQGSWVRGSTAGGCRNFLDTFWTNPQIK--LSLTEKDEGQEECSFLVALMQKDRRKLKRFGANVLTIGYAIYECPDKD-EHLNKDFFRYHASRARSKTFINLREVSDRFKLPPGEYILIPSTFEPHQEADFCLRIFSEKKAITRDMD---GNVDI-DLPEPPKPTPPDQETEEEQ------------RFRALFEQVAGEDMEVTAEELEYVLNAVLQKKKDIKFKKLSLISCKNIISLMDTSGNGKLEFDEFKVFWDKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSHLLQLIVLRYAD-EELQLDFDDFLNCLVRLENA 661
BLAST of Calpain vs. UniProt/SwissProt
Match: sp|Q9D805|CAN9_MOUSE (Calpain-9 OS=Mus musculus OX=10090 GN=Capn9 PE=2 SV=2) HSP 1 Score: 511.916 bits (1317), Expect = 1.802e-169 Identity = 285/694 (41.07%), Postives = 414/694 (59.65%), Query Frame = 2
Query: 389 KVQAKHTPKKKEDVGQQTSYFDLLEQ-LKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIK-NFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNA---FETMELQPED 2437
+ Q + P + F+ L Q +G L+ED DFPA S+F S+ + FVWKRP +I + P+ G +R D+ QG LGDCWLLA +ASL+L + VVP DQ F Y G F F+FW+ +W++VV+DDRLPT+ +LVF+HSAD+NEFWSALLEKAYAK+ G YE+L GGS IEAMEDFTGG+ E ++ +E P + + K K+ +L+GC+I++ ++ E + GLI GHAY++T + +V G ++L+RVRNPWG + EW G WSDSSPEW ++ E++RL +DGEFWM F DF +F K+EIC+L P++L + S RWE+ I GSW + AGGCRNF+D+F TNPQ + + + ++ + T + LMQKD R+ + G L IGY IY+ D + G L++ FF++ ++A+S FIN REV R +LP G Y++IPSTF P++EA+F LRIFSEK T LD +++D D P E+ E++ LF +AG ++A +L +LNA+L K FK L CR++++L D GK+EF++F W +K W F FD D +G +E R AL G + L +++ RY D E + F+D++ ++RL NA F+++ ++ +D
Sbjct: 9 RPQPQPVPGDARTIHSSGQSFEQLRQGCLQSGTLFEDADFPASNVSLFYSERPQ--VPFVWKRPGEIVEKPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALTRVVPQDQGFGSGYAGIFHFQFWQHSEWLDVVIDDRLPTFKDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAENFQI--REAPEDFFEILEKALKRGSLLGCSIDTLNASESEARTSLGLIKGHAYTVTGLDQVN-FHGQRIKLIRVRNPWG-QVEWNGPWSDSSPEWRSVDLEEQKRLGHTALDDGEFWMAFKDFKIHFDKVEICNLTPDALED--SALHRWEVTIHQGSWVRGSTAGGCRNFLDTFWTNPQIK--LSLTERDEGQEGCTFLAALMQKDRRRLKRFGANMLTIGYAIYQCPDKD-GHLSRDFFRYHASLARSKTFINLREVSERFQLPPGDYILIPSTFEPHQEADFCLRIFSEKRAVTRDLD---------------ENIDIDLP-ELPKPTPQEEETEEEQQFRALFQRVAGEDMEVSAEELEYVLNAVLQKKTALKFKRLSLLSCRNIISLMDTSGNGKLEFEEFRVFWDKLKHWMDLFLQFDVDKSGTMSSYELRTALKAAGFQLGGHLLQLIVLRYAD-EDLQLDFDDYLNCLVRLENASRVFQSLSVKNKD 674
BLAST of Calpain vs. TrEMBL
Match: A0A267GW14 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig013791g1 PE=3 SV=1) HSP 1 Score: 742.265 bits (1915), Expect = 0.000e+0 Identity = 373/690 (54.06%), Postives = 487/690 (70.58%), Query Frame = 2
Query: 461 EQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSG----KRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQ--RNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIA------KHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLYM 2494
++ K +G+L+EDPDFPAD S++ S++ Y F WKRPK+IC +P +DGVSRFDV+QG LGDCWLLA VASLS+++ +F VV DQDF NY G FRFRFW+FG+W +V +DDRLPT GKL FM S D NEFWSALLEKAYAK+ G YE+LSGG+Q EAM DFTGGLCET + + P LYN M++ +LMGCAIES ++EG ++NGLI+GHAYS+TDV+KV+ +G LVRVRNPWGNE EWKG WSD S +W ++S+ EK++L + +DGEFWM++ DF+ +F KIEICHLGPES++ + G KRRWEMC+E G W +N++AGGCRN IDSF N QFR+E+VDPD++D EN GT+I+GLMQKD+R+ + FL IGY IY +KDPN L++ FF+F+ + KSP F+NQREVCGRHKLP GHYVIIPSTF+PNEEA F+LRIFSEKPN++ LD N N+ KH +++++P+ + F + AG M I+A QL+ ILN ++IK F+GF LE CRS++AL D + +G MEF +F +W +I++ KT FK++D DGNG FD FE R AL G ++ N +F+ VA RY+DPE+G + FEDFI+ IRL AFET + P+ +EG V D FL LY+
Sbjct: 97 DEQKLSGRLFEDPDFPADNRSIYPSRS---YSRFEWKRPKEICSDPLFISDGVSRFDVKQGELGDCWLLAAVASLSMHKKLFDQVVVPDQDFGANYCGMFRFRFWQFGEWKDVEIDDRLPTKYGKLCFMRSEDRNEFWSALLEKAYAKLNGSYEALSGGNQAEAMVDFTGGLCETFDM--RRAPPNLYNIMVQANNACSLMGCAIESEQLEGHLSNGLITGHAYSVTDVKKVRLNNGMEESLVRVRNPWGNECEWKGDWSDHSHKWSSVSQGEKDKLGMDQRDDGEFWMSYKDFSSHFNKIEICHLGPESMSSSVKGEAAKKRRWEMCLESGQWKRNVSAGGCRNNIDSFHMNEQFRIEIVDPDEDDDENMGTIIVGLMQKDIRKILKGADFLTIGYAIYSIKDPNIPPLDQKFFRFTRQVDKSPSFVNQREVCGRHKLPPGHYVIIPSTFSPNEEAEFILRIFSEKPNESGVLDTPNRMDNVVDGVYGRKHHNTV--MENEEPLKK------------------AFMEFAGSMAKIHAKQLQQILNRSFGREIKEFQGFSLEACRSMLALVDANMSGFMEFDEFKHLWNEIRLLKTIFKEYDADGNGYFDSFELRGALRVAGYNLCNKVFESVAMRYMDPEQGRVCFEDFILLSIRLKTAFETYKAMPKTNEGFGVVKQDHFLLQTLYL 761
BLAST of Calpain vs. TrEMBL
Match: A0A1I8GXB3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 741.495 bits (1913), Expect = 0.000e+0 Identity = 373/690 (54.06%), Postives = 487/690 (70.58%), Query Frame = 2
Query: 461 EQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSG----KRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQ--RNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIA------KHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLYM 2494
++ K +G+L+EDPDFPAD S++ S++ Y F WKRPK+IC +P +DGVSRFDV+QG LGDCWLLA VASLS+++ +F VV DQDF NY G FRFRFW+FG+W +V +DDRLPT GKL FM S D NEFWSALLEKAYAK+ G YE+LSGG+Q EAM DFTGGLCET + + P LYN M++ +LMGCAIES ++EG ++NGLI+GHAYS+TDV+KV+ +G LVRVRNPWGNE EWKG WSD S +W ++S+ EK++L + +DGEFWM++ DF+ +F KIEICHLGPES++ + G KRRWEMC+E G W +N++AGGCRN IDSF N QFR+E+VDPD++D EN GT+I+GLMQKD+R+ + FL IGY IY +KDPN L++ FF+F+ + KSP F+NQREVCGRHKLP GHYVIIPSTF+PNEEA F+LRIFSEKPN++ LD N N+ KH +++++P+ + F + AG M I+A QL+ ILN ++IK F+GF LE CRS++AL D + +G MEF +F +W +I++ KT FK++D DGNG FD FE R AL G ++ N +F+ VA RY+DPE+G + FEDFI+ IRL AFET + P+ +EG V D FL LY+
Sbjct: 92 DEQKLSGRLFEDPDFPADNRSIYPSRS---YSRFEWKRPKEICSDPLFISDGVSRFDVKQGELGDCWLLAAVASLSMHKKLFDQVVVPDQDFGANYCGMFRFRFWQFGEWKDVEIDDRLPTKYGKLCFMRSEDRNEFWSALLEKAYAKLNGSYEALSGGNQAEAMVDFTGGLCETFDM--RRAPPNLYNIMVQANNACSLMGCAIESEQLEGHLSNGLITGHAYSVTDVKKVRLNNGMEESLVRVRNPWGNECEWKGDWSDHSHKWSSVSQGEKDKLGMDQRDDGEFWMSYKDFSSHFNKIEICHLGPESMSSSVKGEAAKKRRWEMCLESGQWKRNVSAGGCRNNIDSFHMNEQFRIEIVDPDEDDDENMGTIIVGLMQKDIRKILKGADFLTIGYAIYSIKDPNIPPLDQKFFRFTRQVDKSPSFVNQREVCGRHKLPPGHYVIIPSTFSPNEEAEFILRIFSEKPNESGVLDTPNRMDNVVDGVYGRKHHNTV--MENEEPLKK------------------AFMEFAGSMAKIHAKQLQQILNRSFGREIKEFQGFSLEACRSMLALVDANMSGFMEFDEFKHLWNEIRLLKTIFKEYDADGNGYFDSFELRGALRVAGYNLCNKVFESVAMRYMDPEQGRVCFEDFILLSIRLKTAFETYKAMPKTNEGFGVVKQDHFLLQTLYL 756
BLAST of Calpain vs. TrEMBL
Match: A0A267DE36 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig013791g4 PE=3 SV=1) HSP 1 Score: 739.184 bits (1907), Expect = 0.000e+0 Identity = 370/688 (53.78%), Postives = 485/688 (70.49%), Query Frame = 2
Query: 461 EQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSG----KRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQ--RNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHL----RDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLYM 2494
++ K +G+L+EDPDFPAD S++ S++ Y F WKRPK+IC +P +DGVSRFDV+QG LGDCWLLA VASLS+++ +F VV DQDF NY G FRFRFW+FG+W +V +DDRLPT GKL FM S D NEFWSALLEKAYAK+ G YE+LSGG+Q EAM DFTGGLCET + + P LYN M++ +LMGCAIES ++EG ++NGLI+GHAYS+TDV+KV+ +G LVRVRNPWGNE EWKG WSD S +W ++S+ EK++L + +DGEFWM++ DF+ F KIEICHLGPES++ + G KRRWEMC+E G W +N++AGGCRN IDSF N QFR+E+VDPD++D EN GT+I+GLMQKD+R+ + FL IGY IY +KDPN L++ FF+F+ + KSP F+NQREVCGRHKLP GHYVIIPSTF+PNEEA F+LRIFSEKPN++ LD N N+ + + +++++P+ + F + AG I+A QL+ ILN ++IK F+GF LE CRS++AL D + +G MEF +F +W +I++ KT FK++D DGNG FD FE R AL G ++ N +F+ VA RY+DPE+G + FEDFI+ IRL AFET + P+ +EG V D FL LY+
Sbjct: 97 DEQKLSGRLFEDPDFPADNRSIYPSRS---YSRFEWKRPKEICSDPLFISDGVSRFDVKQGELGDCWLLAAVASLSMHKKLFDQVVVPDQDFGANYCGMFRFRFWQFGEWKDVEIDDRLPTKYGKLCFMRSEDRNEFWSALLEKAYAKLNGSYEALSGGNQAEAMVDFTGGLCETFDM--RRAPPNLYNIMVQANNACSLMGCAIESEQLEGHLSNGLITGHAYSVTDVKKVRLNNGMEESLVRVRNPWGNECEWKGDWSDHSHKWSSVSQGEKDKLGMDQRDDGEFWMSYKDFSSQFNKIEICHLGPESMSSSVKGEAAKKRRWEMCLESGQWKRNVSAGGCRNNIDSFHMNEQFRIEIVDPDEDDDENMGTIIVGLMQKDIRKILKGADFLTIGYAIYSIKDPNIPPLDQKFFRFNRQVDKSPSFVNQREVCGRHKLPPGHYVIIPSTFSPNEEAEFILRIFSEKPNESGVLDTPNTMDNVVDGVYGRKHHSTVMENEEPLKK------------------AFMEFAGSTAKIHAKQLQQILNRSFGREIKEFQGFSLEACRSMLALVDANMSGFMEFDEFKHLWNEIRLLKTIFKEYDADGNGYFDSFELRGALRVAGYNLCNKVFESVAMRYMDPEQGRVCFEDFILLSIRLKTAFETYKAMPKTNEGFGVVKQDHFLLQTLYL 761
BLAST of Calpain vs. TrEMBL
Match: A0A1I8JIV8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 706.827 bits (1823), Expect = 0.000e+0 Identity = 365/684 (53.36%), Postives = 467/684 (68.27%), Query Frame = 2
Query: 461 EQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSG----KRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQ--RNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLYM 2494
++ K +G+L+EDPDFPAD S++ S++ Y F WKRPK+IC +P +DGVSRFDV+QG LGDCWLLA VASLS+++ +F VV DQDF NY G FRFRFW+FG++ DDRLPT GKL FM S D NEFWSALLEKAYAK+ G YE+LSGG+Q EAM DFTGGLCET + + P LYN M++ +LMGCAIES ++EG ++NGLI+GHAYS+TDV+KV+ +G LVRVRNPWGNE EWKG WSD S +W ++S+ EK++L + +DGEFWM++ DF+ +F KIEICHLGPES++ + G KRRWEMC+E G W +N++AGGCRN IDSF N QFR+E+VDPD++D EN GT+I+GLMQKD+R+ + FL IGY IY +KDPN L++ FF+F+ + KSP F+NQREVCGRHKLP GHYVIIPSTF+PNEEA F+LRIFSEKPN++ LD T A M I+A QL+ ILN ++IK F+GF LE CRS++AL D + +G MEF +F +W +I++ KT FK++D DGNG FD FE R AL G ++ N K VA RY+DPE+G + FEDFI+ IRL AFET + P+ +EG V D FL LY+
Sbjct: 92 DEQKLSGRLFEDPDFPADNRSIYPSRS---YSRFEWKRPKEICSDPLFISDGVSRFDVKQGELGDCWLLAAVASLSMHKKLFDQVVVPDQDFGANYCGMFRFRFWQFGEF-----DDRLPTKYGKLCFMRSEDRNEFWSALLEKAYAKLNGSYEALSGGNQAEAMVDFTGGLCETFDM--RRAPPNLYNIMVQANNACSLMGCAIESEQLEGHLSNGLITGHAYSVTDVKKVRLNNGMEESLVRVRNPWGNECEWKGDWSDHSHKWSSVSQGEKDKLGMDQRDDGEFWMSYKDFSSHFNKIEICHLGPESMSSSVKGEAAKKRRWEMCLESGQWKRNVSAGGCRNNIDSFHMNEQFRIEIVDPDEDDDENMGTIIVGLMQKDIRKILKGADFLTIGYAIYSIKDPNIPPLDQKFFRFTRQVDKSPSFVNQREVCGRHKLPPGHYVIIPSTFSPNEEAEFILRIFSEKPNESGVLDTPTEWTTWW---------------------------------------TACSMAKIHAKQLQQILNRSFGREIKEFQGFSLEACRSMLALVDANMSGFMEFDEFKHLWNEIRLLKTIFKEYDADGNGYFDSFELRGALRVAGYNLCN---KSVAMRYMDPEQGRVCFEDFILLSIRLKTAFETYKAMPKTNEGFGVVKQDHFLLQTLYL 723
BLAST of Calpain vs. TrEMBL
Match: A0A2R2MIP4 (calpain-A OS=Lingula unguis OX=7574 GN=LOC106156774 PE=3 SV=1) HSP 1 Score: 690.263 bits (1780), Expect = 0.000e+0 Identity = 351/707 (49.65%), Postives = 476/707 (67.33%), Query Frame = 2
Query: 419 KEDVGQQTSYFDLLEQLKGT----GKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDF-DKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESA--KIEGVMANGLISGHAYSITDVQKVKQTDGTV---VELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSG---KRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQR---NLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
K D+ + E+LK G+L+EDP+FPA S+F S+A R F WKRP ++C NP LF G SRFDVQQG LGDCWLLA +ASLSL + + VVP +Q F D Y G F F+FWRFGKWV+VVVDDRLPTY +LVFMHSAD+NEFWSA+LEKAYAK+CG YE+L GGS EAMEDFTGG+ E L + + P L+ M K ++S+LMGC+IE++ ++E ++NGLI GHAYSIT V+ V + + L+R+RNPWGNE EWKG W D SPEW IS++E++ + F +DGEFWM+F DFA NF K+EIC+LGP++L ED G K++WE E+G W + +NAGGCRN++D+F TNPQ+R+ V DPD++D +NT T+I+GLMQKD R++ L L +GY IY LKDP+CG L+ FFK++ + AKSP FIN REVCGRHKLP G Y IIPSTF PN+E +F+LRIF+EK N +D K G +++ + PV+ + ++L + F+ +AG I+A +LRD+LNA +++ K F GF +E CRS+VA+ D D +GK+ F++F K+W D+++WK+ FK FD D +G + +E RNAL NVG+ VSN F + R+ D + G I F+DFI+ +RL F+T + G FT D+F+++ +Y
Sbjct: 105 KPDIPTGATTVQNFEELKAQCLREGRLFEDPEFPAINQSIFYSRAPP--RPFQWKRPHELCSNPDLFVGGASRFDVQQGELGDCWLLAAIASLSLNKELLHKVVPPNQSFADGEYAGIFVFKFWRFGKWVDVVVDDRLPTYNNRLVFMHSADSNEFWSAMLEKAYAKLCGSYEALKGGSTCEAMEDFTGGVTEMFDLRQPQ-PPNLFQIMQKAFERSSLMGCSIEASPHQLEAELSNGLIMGHAYSITSVKMVDIKTPRMAGKIPLIRIRNPWGNEAEWKGAWGDKSPEWNFISEAERQEIGLQFDDDGEFWMSFKDFASNFQKLEICNLGPDALTEDELGTTSKKKWEASSEEGGWKRRVNAGGCRNYLDTFWTNPQYRISVTDPDEDDDDNTCTIIVGLMQKDRRKKKKEGLDLLTVGYAIYLLKDPDCGPLDLRFFKYNASFAKSPSFINMREVCGRHKLPPGSYAIIPSTFEPNQEGDFLLRIFTEKANTAAAIDEKTGISDVQNGFPHKPGPQA--PVTPQD-------EQQTQKLRETFHRVAGEDMEIDAHELRDVLNAAFMREFK-FDGFSMETCRSMVAMMDVDQSGKLGFEEFQKLWNDLRLWKSVFKQFDKDMSGTLNSYELRNALNNVGMRVSNQTFNALVMRFAD-KHGAIFFDDFILCAVRLKTMFDTFTSLDPNKTGAATFTLDQFIQTTMY 797
BLAST of Calpain vs. Ensembl Cavefish
Match: zgc:55262 (calpain-1 catalytic subunit-like [Source:NCBI gene;Acc:103042634]) HSP 1 Score: 486.878 bits (1252), Expect = 1.368e-160 Identity = 275/669 (41.11%), Postives = 397/669 (59.34%), Query Frame = 2
Query: 485 LWEDPDFPADTSSVFVSKA---SKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAK---IEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMR-QRNLG--FLVIGYVIYKLKDPNCGL----LNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILV--KDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEG 2446
L+ED FPA +SS+ ++ S + WKRP +IC PQ DG +R D+ QGALGDCWLLA +ASL+L + + VVP Q F Y G F F+FW+FG+W++VV+DDRLP GKL+F+HSA+ EFWSAL+EKAYAK+ G YE+LSGGS E EDFTGG+ E +L K+ P LYN + + + +L+GC+I+ +E V L+ GHAYS+T V +V GT +LVR+RNPWG E EW G WSD+S EW+++ S + RL + DGEFWM+F DF + F+++EIC+L ++L + ++W C+ +G W + AGGCRNF +F NPQF+V + PD S + + ++ LMQKD R +R G IG+ IY++ G L + FF + + A+S FIN REV R +LP G Y+I+PSTF P +EA+F+LR+FSEKP + LD DDV ++ P + ++ + LF +AG I+ +L+ ILN I+ KD+K GF E CRS++ L D D +GK+ +F +W+ IK + T F+ FD D +G +E R AL G ++N LF+++ RY + E + F++F+ ++RL F+T + D +G
Sbjct: 50 LFEDSLFPASSSSLGFNELGPRSSKTQGVQWKRPPEICSRPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDNLLHRVVPHGQSFGSGYAGIFHFQFWQFGEWMDVVIDDRLPVKDGKLLFVHSAEGGEFWSALVEKAYAKLNGCYEALSGGSTSEGFEDFTGGVTEMYEL--KKAPSDLYNIIQRAVDRGSLLGCSIDITSKFDMEAVTFKKLVKGHAYSVTAVHEVVYR-GTPTKLVRIRNPWG-EVEWTGAWSDNSREWDSVDSSSRARLQVR-SEDGEFWMSFRDFLQEFSRLEICNLTADAL--EAGQVKKWSTCLYNGEWRRGSTAGGCRNFPATFWLNPQFKVNLTHPDKAGSSDC-SFLVALMQKDRRKKRKEGKDMETIGFAIYEVPGQYVGQSGVHLKRDFFLTNASSARSELFINLREVSSRFRLPAGEYIIVPSTFEPQKEADFVLRVFSEKPASAEELD---------------DDVTAELPSENQ-----LDESQIDAGFKSLFRQLAGSDMEISVTELQTILNRIISKHKDLKT-DGFGPEACRSMINLMDTDGSGKLGLTEFHVLWEKIKRYLTVFRQFDLDKSGTMSSYEMRMALEAAGFKLTNHLFQLIILRYTE-EDLTVDFDNFVTCLVRLETMFKTFKTLDTDADG 688
BLAST of Calpain vs. Ensembl Cavefish
Match: capn3b (calpain 3b [Source:ZFIN;Acc:ZDB-GENE-041001-149]) HSP 1 Score: 478.019 bits (1229), Expect = 8.283e-157 Identity = 280/703 (39.83%), Postives = 417/703 (59.32%), Query Frame = 2
Query: 422 EDVGQQTSYFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDG--TVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTG---TVIIGLMQKD-MRQRNLG--FLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFK--GFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
+D + + +L ++ L++DP FPAD SS++ ++ K + VWKRP +ICKNPQ G +R D+ QG LGDCWLLA +A L+L E + VVP DQ F +NY G F F+FWR+G+WV+VVVDDRLPT LVF S + NEFWSALLEKAYAK+ G YE+L GG+ +EAMEDFTGG+ E ++++ R + YN M K + +LMGC+I+S + E GL+ GHAYS+T V++ KQ DG T + LVR+R+PWG S S +W +++ SE+++ GEFWM F++F + FTK+EIC+L P++L +D K W++ + +G W + +AGGCRN+ D+F TNPQ+R+ + + DD++ ++ TV++ LMQK R+R G L IG+ IY++ N L K FF ++ + A+ +IN REV R L G YVI+PSTF+PN E++F+LR+FSE KT T ++ +++D V +S ++ EK+ +F +AG INA +LR + N +L K K K GF L+ CRS++AL D D TG++ ++F +W I+ WK F +D D + FE RNA+ + G ++ L ++A RY E + F+ +I ++RL F + D +G + E+L+ +Y
Sbjct: 58 KDAKRLKDFLELRDKYVRKKVLFKDPLFPADDSSLYYNQ--KFPLKLVWKRPSEICKNPQFIVGGANRTDICQGDLGDCWLLAAIACLTLSETLLYRVVPADQSFSENYAGIFHFQFWRYGEWVDVVVDDRLPTSNNHLVFTRSGNKNEFWSALLEKAYAKLHGTYEALKGGNTLEAMEDFTGGVTEFYEISDAPREI--YNIMKKALNRGSLMGCSIDSLVPSASETKTDTGLVRGHAYSVTAVEQGKQEDGSDTRIRLVRLRDPWGVAP----PPSRMSCDWSSLATSEQDQQRLKPVEAGEFWMGFEEFQQTFTKLEICNLTPDALQDDELLK--WDVSVNEGRWVRGCSAGGCRNYPDTFWTNPQYRLRLTEEDDDEPDHGEKGCTVVVALMQKGRRRERCTGGTLLTIGFAIYEMHG-NKQHLPKDFFLYTASKARCKSYINLREVTERFCLSPGEYVIVPSTFDPNNESDFILRVFSE---KTSTSELSGAV------------IEADPCVLPKS---EDEESDEEKQFRAMFQQIAGDDMQINAKELRSVFNKLLTK-YKGLKTEGFSLDSCRSMIALMDTDGTGRLNLEEFKHLWSKIQQWKLIFSRYDRDKSSSISSFEMRNAVADAGFQLNPQLHDIIAMRYAS-EHLYLDFDSYICCLVRLEGMFRAFKAFDIDGDGIIKLNSLEWLQLTMY 729
BLAST of Calpain vs. Ensembl Cavefish
Match: capn1a (calpain-1 catalytic subunit-like [Source:NCBI gene;Acc:103024720]) HSP 1 Score: 468.003 bits (1203), Expect = 2.864e-153 Identity = 270/717 (37.66%), Postives = 427/717 (59.55%), Query Frame = 2
Query: 383 ASKVQAKHTPKKKEDVGQQTSYFDLLEQ--LKGTGKLWEDPDFPADTSSVF---VSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIE---SAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXN--TGTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCGL----LNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILV--KDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFL 2476
AS+++++ ++ V Q F+ L+Q ++G G L+EDP FPA+ S+ + S W RP ++C +PQ G +R D+ QGALGDCWLLA +ASL+L E + VVP Q F +Y G F F+FW+FG+WV++V+DDRLP G+L+F+HSA+ NEFWSAL+EKAYAK+ G YE+LSGGS E EDFTGG+ E +L ++ P LY + K + +L+GC+I+ + +E V L+ GHAYS+T +++V G + L+R+RNPWG + EW G WSD+S EW+ I +E+E LC DGEFWM+F++F + F+++EIC+L P++L++D G W GSW + AGGCRN ++F NPQ+++ +++ DD+ ++ + ++ L+QKD R+ R G IG+ +Y++ + G L K+FF ++ A+S FIN REV R +LP G Y+I+PSTF P++EA+F+LR+F+EK ++T+ LD D++ +D EE ++ + +F +AG I+ +LR ILN +++ KD+K GF +E CR++V L D D + ++ +F +W I+ W ++ FD D +G +E R A+ + G ++N L +++ RY + E VI F++FI +++L F + + ++ G E+L
Sbjct: 13 ASRLKSEGMGSYEQAVHFQNQDFEELKQECVEG-GFLFEDPCFPAEPPSLGFKELGPHSSKTSGVEWMRPTELCDDPQFILGGATRTDICQGALGDCWLLAAIASLTLNEKLLHRVVPHGQSFQDDYAGIFHFQFWQFGEWVDIVIDDRLPVKDGELMFVHSAEGNEFWSALIEKAYAKLNGSYEALSGGSTTEGFEDFTGGVSEMYEL--RKPPRDLYRIISKALELGSLLGCSIDITSTFDMEAVTFKKLVKGHAYSVTGLRQVN-FRGDLQRLIRIRNPWG-QVEWTGAWSDNSSEWDEIDPTEREDLCLQM-EDGEFWMSFNEFLRQFSRLEICNLTPDALSDD--GLSHWNTIQFHGSWRRGSTAGGCRNHPNTFWINPQYKITLLEEDDDPEDDEVACSFLVALLQKDRRRYRRQGQDMHTIGFAVYEIPEEFRGCQNVHLKKNFFLSHSSCARSETFINLREVSARLRLPPGEYLIVPSTFEPSKEADFVLRVFTEKQSETEELD---------------DEISADLEDEEEI-----TEDDIDDSFKSMFAQLAGEDMEISVHELRTILNRVVLRHKDLKT-DGFSMESCRTMVNLMDKDGSARLGLVEFQILWNKIRKWLVIYRQFDLDKSGTMSSYEMRLAVESAGFKLNNRLNQILVARYAENE--VIDFDNFICCLVKLEAMFRSFQQLDKEGSGEVEMNITEWL 698
BLAST of Calpain vs. Ensembl Cavefish
Match: si:dkeyp-50d11.2 (si:dkeyp-50d11.2 [Source:ZFIN;Acc:ZDB-GENE-091113-22]) HSP 1 Score: 464.922 bits (1195), Expect = 4.372e-152 Identity = 273/704 (38.78%), Postives = 403/704 (57.24%), Query Frame = 2
Query: 416 KKEDVGQQTSYFDLL----EQLKGT----GKLWEDPDFPADTSSVFVSKASKG---YREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAK---IEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCGL----LNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILV--KDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVF 2458
+ E +GQ L E L+ + +L+ED FPA SS+ + + G R W RP + C +P DG +R D+ QGALGDCWLLA +A L+L EP+ VVP Q F Y G F F+FW+FG WV+VV+DDRLP GKL+F+HS + EFWSAL+EKAYAK+ G YE+LSGG E EDFTGG+ E +L ++ P L++ + + ++ +L+GC+I+ +E V L+ GHAYS+T V +V + G + +L+R+RNPWG E EW G WSD S EW I+ + + RL N A DGEFWM+F DF K F+++EIC+L ++L S ++W + G W + AGGCRN+ +F NPQ+++ + DPD E + + ++ LMQK+ RQ R G IG+ IY++ G+ L + FF +T+ A+S FIN REV R LP G Y+I+PSTF P +E +F+LR+FSEK + LD D++ +D P + +++ + LF +AG I+ +L+ ILN ++ KD+K GF E CR +++L D G++ DF +W+ IK + T F++FD D +G +E R AL + G ++N LF+++ RY P+ V F++F+ +IRL + F+T D +G F
Sbjct: 19 RSEGIGQNHKALKFLGQDFESLRASCLQSRRLFEDETFPAQPSSLGFKELAPGSAKTRGVRWMRPTEFCDDPHFIVDGATRTDICQGALGDCWLLAAIACLTLNEPLLHRVVPHGQSFHYQYAGIFHFQFWQFGDWVDVVIDDRLPVRDGKLLFVHSDEGAEFWSALVEKAYAKLNGCYEALSGGCTSEGFEDFTGGVTEMYEL--RKAPPNLFSIIRRAVERGSLLGCSIDITSFFDMEAVTFKKLVKGHAYSVTGVDEV-EFRGNLTKLIRIRNPWG-EVEWTGAWSDESREWSGINPALRSRL-HNRAEDGEFWMSFSDFLKEFSRLEICNLTADALQS--SELKKWNSSLYTGEWRRGSTAGGCRNYPATFWINPQYKIMLKDPDTEGQDGC-SFLVALMQKNRRQLRREGKDMETIGFAIYEVPLQFLGMAGVHLKRDFFLTNTSSARSELFINLREVSSRFCLPAGEYIIVPSTFEPQKEGDFVLRVFSEKAADSQELD---------------DELTADLPEED-----ALPESEIDEGFKALFAKLAGKDMEIDVPKLQMILNRVISKHKDLKT-DGFGKEACRCMISLMDTSGCGRLGLTDFHVLWEKIKRYLTIFREFDLDKSGTMSSYEMRLALESAGFKLTNNLFQLIILRYAKPDLNV-DFDNFVTCLIRLESMFKTFRTMDTDADGVVTF 692
BLAST of Calpain vs. Ensembl Cavefish
Match: zgc:55262 (calpain-1 catalytic subunit-like [Source:NCBI gene;Acc:103042634]) HSP 1 Score: 460.299 bits (1183), Expect = 9.622e-151 Identity = 272/693 (39.25%), Postives = 392/693 (56.57%), Query Frame = 2
Query: 416 KKEDVGQQTSYFDLL----EQLKG----TGKLWEDPDFPADTSSVFVSKA---SKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAK---IEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMR-QRNLG--FLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDH------TGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMEL 2425
+ E +GQ + L E LK + L+ED FPA +SS+ ++ S + WKRP +IC PQ DG +R D+ QGALGDCWLLA +ASL+L + + VVP Q F Y G F F+FW+FG+W++VV+DDRLP GKL+F+HSA+ EFWSAL+EKAYAK+ G YE+LSGGS E EDFTGG+ E +L K+ P LYN + + + +L+GC+I+ +E V L+ GHAYS+T V GT +LVR+RNPWG E EW G WSD+S EW+++ S + RL + DGEFWM+F DF + F+++EIC+L ++L + ++W C+ +G W + AGGCRNF +F NPQF+V + PD S + + ++ LMQKD R +R G IG+ IY+ + L + FF + + A+S FIN REV R +LP G Y+I+PSTF P +EA+F+LR+FSEKP + LD DDV ++ P + ++ + LF +AG I+ +L+ ILN I+ K E SL L +D +GK+ +F +W+ IK + T F+ FD D +G +E R AL G ++N LF+++ RY + E + F++F+ ++RL F L
Sbjct: 19 RDEGLGQNHNAIKFLGQDYEMLKARCLQSRSLFEDSLFPASSSSLGFNELGPRSSKTQGVQWKRPPEICSRPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDNLLHRVVPHGQSFGSGYAGIFHFQFWQFGEWMDVVIDDRLPVKDGKLLFVHSAEGGEFWSALVEKAYAKLNGCYEALSGGSTSEGFEDFTGGVTEMYEL--KKAPSDLYNIIQRAVDRGSLLGCSIDITSKFDMEAVTFKKLVKGHAYSVTAV----VYRGTPTKLVRIRNPWG-EVEWTGAWSDNSREWDSVDSSSRARLQVR-SEDGEFWMSFRDFLQEFSRLEICNLTADAL--EAGQVKKWSTCLYNGEWRRGSTAGGCRNFPATFWLNPQFKVNLTHPDKAGSSDC-SFLVALMQKDRRKKRKEGKDMETIGFAIYEYVGQSGVHLKRDFFLTNASSARSELFINLREVSSRFRLPAGEYIIVPSTFEPQKEADFVLRVFSEKPASAEELD---------------DDVTAELPSENQ-----LDESQIDAGFKSLFRQLAGSDMEISVTELQTILNRIISKR---------EFSDSLYCLIFFDRCIQTDGSGKLGLTEFHVLWEKIKRYLTVFRQFDLDKSGTMSSYEMRMALEAAGFKLTNHLFQLIILRYTE-EDLTVDFDNFVTCLVRLETMFSKWTL 670
BLAST of Calpain vs. Ensembl Sea Lamprey
Match: capn9 (calpain 9 [Source:ZFIN;Acc:ZDB-GENE-010724-2]) HSP 1 Score: 492.271 bits (1266), Expect = 3.123e-165 Identity = 262/586 (44.71%), Postives = 371/586 (63.31%), Query Frame = 2
Query: 566 WKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRF--RFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIE---SAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGT----VIIGLMQKDMRQRNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIK-NFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVG 2293
W ++I +NP+ DG +R D++QGALGDCWLLA +ASL+L + + VVP Q+F Y G F F +FW FG+W++V +DDRLPT G+LVF+HSAD EFWSALLEKAY+K+ G YE+L GGS EAMEDFTGG+ E + L K P L+ M K ++ ++MGC+I+ +++ E +NGL+ GHAYSIT V +V G V+L+R+RNPWG E EW G WSD +PEW+ + + E+ RL A DGEFWM+FDDF ++FTK+EIC+L P+ + + R W + + DG W K AGGCRNF ++F TNPQFR+++ + DDE TG+ I+ LMQK+MR++ L +GY IYK+ D G + K FF ++ + A+SP FIN REV R +LP G YVI+PSTF P++EA+F++R+F+EKPN++ D K G + D D +P +E +++ + LF +AG I+ +L+D+LN I K + GF + CRS+VAL D D +GK+ ++F +W IK W+ F+ D D +G +E +NAL G
Sbjct: 4 WGLSREIVQNPEFIVDGATRTDIKQGALGDCWLLAAIASLTLNDQLLAKVVPPGQNFRDGYAGIFHFQQKFWHFGEWMDVFIDDRLPTKNGQLVFVHSADKREFWSALLEKAYSKLNGSYEALKGGSIAEAMEDFTGGVYELLDL--KTAPPDLFRIMCKARQKGSMMGCSIDVTNASESEARTSNGLVKGHAYSITGVDEVNYR-GQKVKLLRIRNPWG-EVEWNGAWSDHAPEWKALDQGERNRLQQVMAEDGEFWMSFDDFMRHFTKMEICNLTPDRVERE-GDPRYWTVTVHDGRWLKGCTAGGCRNFPETFWTNPQFRLKLHNEDDEAD-ETGSKACSFIVALMQKNMRKQQRNMLTMGYAIYKIPD-QTGPMPKEFFLYNASKARSPSFINLREVSQRFRLPAGEYVIVPSTFEPHQEADFLVRVFAEKPNRSKESDDKIGA--------EIDLNDVAKPGTE---------TEDDRKFKTLFGRLAGEDMEISPKELQDMLNKITAKHGELESTGFSMHACRSIVALTDVDGSGKLGLQEFRILWNKIKKWQAIFRKHDEDLSGTMSSYEMKNALNAAG 565
BLAST of Calpain vs. Ensembl Sea Lamprey
Match: capn3a (calpain 3a, (p94) [Source:ZFIN;Acc:ZDB-GENE-040912-97]) HSP 1 Score: 476.093 bits (1224), Expect = 9.669e-156 Identity = 284/756 (37.57%), Postives = 422/756 (55.82%), Query Frame = 2
Query: 485 LWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAI--------------------------------------ESAK---------IEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNT--GTVIIGLMQKDMR-QRNLG--FLVIGYVIYKLKDPNCG---LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNG----------------KTNIAKHLRDTDDVDSDQP--------------VSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILV--KDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
L+EDPDFPA +S++ S+ + +F W RPK++C P+ + + +G LGDCW+LA +A +++ E + V+P Q F++NY G F F+FWRFG+WV+VVVDDRLPT+ +LVF+ SA+ NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E ++ K P LY M+K + +LMGC+I E AK E GL+ HAYSIT + +VK +V+LVR+RNPWG+ EW G WSD+S EW I+ SEK+RL A DGEFW++F DF +NFTK+EIC+L P++L +D + K W + + +G W + +AGGCRN+ D+F TNPQ+R+++ D DD+ +N + I+ LMQK+ R +R G L IG+ IY++ + G L K FF ++ + A+ +IN REV R +LP G YVI+PSTF ++E F+LR+F+EK N ++ +D G K + + D +P ++E K L +K+ + +F +AG + A L +ILN ++ KD+K GF LE CR++VAL D D +GK+ + DF +W +K W+ F+ +D D +G +E R A+ G ++N L++++ R+ D E I F++FI +IRL F + +D +G E+L+ +Y
Sbjct: 66 LFEDPDFPAHNNSIYFSQNAP--MQFQWMRPKELCV-PRSIVIEANHTHICEGDLGDCWVLAAIACMTMNEELLARVIPAGQSFEENYAGIFHFQFWRFGEWVDVVVDDRLPTFNNQLVFVQSAERNEFWSALLEKAYAKVHGSYEALKGGNTTEAMEDFTGGVTEYFEM--KNPPKDLYKIMVKATSRRSLMGCSIDDGMCWRCGVPAPDSIGLVRSNMSPPILRHIQLTPIGIEDAKKRKIAMLTHAESRTPMGLVRNHAYSITGLDEVKHR-SKIVQLVRLRNPWGH-VEWIGPWSDASKEWNEITPSEKQRLQLISAEDGEFWISFQDFCRNFTKLEICNLSPDALADDRTNK--WTVSVSEGRWVRGCSAGGCRNYPDTFWTNPQYRLQLYDEDDDPDDNELLCSFIVALMQKNRRKERRQGSTMLTIGFAIYEVTEELRGSREHLPKDFFLYNASRARCNSYINLREVTERFRLPPGEYVIVPSTFEAHQEGEFLLRVFAEKHNVSEEMDDIIGVKKKSKKKKNKPIVFISDRTKKGQQQPESPDESEPEDGNEEESEPKSNKLAEPENPKEPDLTPEDKQFKTIFKQLAGDDMEVGAADLHNILNKVITKHKDLKT-SGFSLESCRTMVALMDTDLSGKLGYNDFKYLWDKLKFWQQIFRKYDSDKSGSMTSYEMRLAINEAGYQLNNQLYQIITLRHAD-ENLNIGFDNFIACLIRLEAMFRAFKAFDKDGDGIIRLNVLEWLQLTMY 810
BLAST of Calpain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003998.1 (pep scaffold:Pmarinus_7.0:GL501031:16584:50555:1 gene:ENSPMAG00000003440.1 transcript:ENSPMAT00000003998.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 471.085 bits (1211), Expect = 8.059e-155 Identity = 275/717 (38.35%), Postives = 386/717 (53.84%), Query Frame = 2
Query: 569 KRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCE---------------------------------TIKLNEKERPVGLYNQMLKFAKQST-------------------LMGCAIESAK---IEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQF--RXXXXXXXXXXXXNTGTVIIGLMQKDMRQRNLGFLVIGYVIYKL---KDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNK----TDTLDIKNGK----------TNIA------------KHLRDTDDVDSDQPVSEE---SXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFK--GFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEG 2446
+ P++IC NPQ +G +R D++QGALGDCWLLA +ASL+L+ + VVP Q F Y G F F+FW+FG+WV+VV+DD LPT KL F+HS D NEFWSALLEKAYAK+ G YE+L GGS EAMEDFTGG+ E T K R + + F S+ L IE +E + NGL+ GHAYSIT + +V +G V LVR+RNPWG E EW G WSD+S EW + SE+ RL A DGEFWM+F DF +NF+K+EIC+L P++L SG R W + +G W + AGGCRN+ +F TNPQF R+ D D +D E + I+ LMQK+MR++ L IGY IY++ P C L FF +++ A+S FIN REV R +LP GHYVI+PST+ P++EA+F+LR++ EK N+ T + G+ +N+ +H+ D + + V E S ++ ++F +AG I+ +L+D+LN + K +K FK GF L C+S+VAL D D +GK+ +F +W+ IK+W+ F+ +D D +G +E R AL G ++N +++++ RY D E I F D+I ++RL F +D G
Sbjct: 1 RAPQEICYNPQFIVNGATRTDIKQGALGDCWLLAAIASLTLHPRLLARVVPEGQSFSDGYAGIFHFQFWQFGEWVDVVIDDLLPTQNNKLAFLHSTDRNEFWSALLEKAYAKLNGSYEALKGGSIAEAMEDFTGGVFEFYNLKKAPPDLYRIMRKGLEKASMMGCSIDEGSTFKYRSTPRAINMSKMTATFRAWSSGIRRFAERFLPSRLGHARLLASSGIEITSMFDLEARLPNGLVKGHAYSITGLDQVF-FEGQDVRLVRIRNPWG-EVEWNGAWSDNSWEWRVVDVSERRRLEQVRAEDGEFWMSFSDFKRNFSKMEICNLSPDTLRTQ-SGSRHWTVTAHEGRWLRGCTAGGCRNYAGTFWTNPQFHLRLHEEDDDPDDDEVACSFIVALMQKNMRRQKRDLLTIGYAIYEIPLKAPPGCTHLPGEFFVRNSSRARSHNFINLREVSDRFRLPPGHYVIVPSTYEPHQEADFLLRVYCEKMNRSRGETKHNSVSKGRPIFRGAFKQVSNLGGCGGPNCYRVVDEHIADEILKSNSERVGEAVQPSMPVEVVETDEDRRFTQVFKKLAGEDMEISPRELQDLLNKVTAK-LKVFKSDGFSLYACQSMVALMDSDGSGKLGLPEFKLLWEKIKMWQAIFRKYDEDRSGTMSSYEMRAALNAAGFHLNNQMYQLITMRYAD-ESMTIKFNDYITCMVRLEAMFRIFHALDKDKRG 712
BLAST of Calpain vs. Ensembl Sea Lamprey
Match: capn1a (calpain 1, (mu/I) large subunit a [Source:ZFIN;Acc:ZDB-GENE-040426-1263]) HSP 1 Score: 382.489 bits (981), Expect = 2.873e-121 Identity = 247/688 (35.90%), Postives = 365/688 (53.05%), Query Frame = 2
Query: 485 LWEDPDFPADTSSVFV----SKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIE----SAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCR--NFIDSFVTNPQFRXXXXXXXXXXXXNTGT-VIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHL-RDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKG-----FDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFL 2488
L+EDPDFPA SS+ + + ++ WKRP ++ ++P+ G +R D+ QG L DCWLL+ +ASL+LY + VVP Q F ++Y G F FRF ++G+WV+V VDDRLPT G+LVF+ S NEFWSALLEKAYAK+ G YE+L+ G +EA+ DFTGG+ ET +N P ++ + + K+ ++ C IE S + E V GL+ GHAYSIT V K+K G V+L+RVRNP+G + E+ G WSD S W + + +++RL A DGEFW++ DF KNF +EICH G E E K W + + W K +NAGG R F N QF + +++ D+ G ++ L QK R+ R +G F+ IG IYK P L + K + +SP FI +REV R LP G+Y+IIP T + +EA F+LRIF EK + L + + + R ++ EES F +AG ++I A D L A++ +I+ + F +E CR LVAL D D++G++ +++F+ +W+ IK+ +K D D +G E AL + V + +++ R+ +P I+F+ F+ + ++ AF G EFL F
Sbjct: 43 LFEDPDFPASLSSLHIRVQPTVITQSNNFIEWKRPWELVRHPEFIVSGATRADINQGTLPDCWLLSAIASLTLYPDLLAKVVPMGQSFHEDYAGIFLFRFCQYGEWVDVHVDDRLPTKKGELVFVQSGTGNEFWSALLEKAYAKLNGCYEALAYGFPVEALCDFTGGVGETFTMNNA--PRDYWDGISRAIKKGAIICCGIETLAASGRGEKVTPMGLVEGHAYSITGVIKLK-FPGKTVQLIRVRNPYG-KVEYTGPWSDGSTTWNEVPEEDQKRLHVR-AEDGEFWISDKDFRKNFHLVEICHQGSEV--EIEHRKNLWICELHNSMWLKGLNAGGSRRHRVFKEFPLNYQFFLTLLEEDNVMDVCHGCHFLLSLTQKHRRKFRKIGIDFVPIGIYIYKAVVPGKKLTPQQLRK-EKPLVESPEFIARREVTMRMTLPPGNYIIIPCTESAGQEAQFLLRIFMEKAQQHQGL---ASQLEVTPYFPRMVSPPPAENFDMEES-----------------FLQVAGKDKLIKA----DKLQALITLEIQKYTTVVVDKFSMESCRVLVALFDIDNSGQLGYEEFLLVWEFIKMTLKTYKMCDVDKSGTLSSTELPMALEHSRFKVGKLMQRLIMMRFSEPNL-TISFDSFVCLLAKMYTAFNIFISLDAAGTGTITLNLHEFLSPFF 697
BLAST of Calpain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007888.1 (pep scaffold:Pmarinus_7.0:GL477427:174304:193458:1 gene:ENSPMAG00000007039.1 transcript:ENSPMAT00000007888.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 328.946 bits (842), Expect = 1.350e-102 Identity = 202/567 (35.63%), Postives = 305/567 (53.79%), Query Frame = 2
Query: 902 KMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAI---ESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTG--TVIIGLMQKDMRQ-RNLG--FLVIGYVIYKLKDPNCGL----LNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEK------------------PNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXX----XXXFNDMAGPMEVINACQLRDILNAILVK--DIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGV-ITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
++ G YE+LSGGS E EDFTGG+ E+I+LN+ P L+ + + + +LMGC+I A E + + L+ GHAYS+T +V G +V LVR+RNPWG + EW G WSD++P+W +S ++ RL + DGEFWM+F+DF + F+++EIC+L P++L++D G W +GSW + +AGGCRNFI +F ++ + DD+ +N T I+ LMQK+ R+ R G L +GY IY++ + G L K +F++ ++ A+S FIN REV R LP G Y+++PSTF PN+EA+F+LRIFSEK P T T + H R+ + P S KLF+ +AG + I+A +L+ ILN ++ K GF LE CRS+V+L D D T K+ K+F +WK IK W+ F ++D D G + E R AL G ++ L ++V RY D E G+ I F++++ ++RL F T + +D +FL ++
Sbjct: 1 RLQGSYEALSGGSTTEGFEDFTGGVAESIELNKP--PSNLWRMLQQATLRGSLMGCSINITSQADSEAITSRKLVKGHAYSLTGTTEVDY-QGQMVRLVRIRNPWG-QVEWTGAWSDNAPQWGYVSDEDRGRLQVS-QEDGEFWMSFEDFLRQFSRLEICNLTPDALDDDSLG--HWSANNFEGSWVRGCSAGGCRNFIVHLWEKWKFHLKHTEEDDDPHDNEAGCTFIVALMQKERRKKRRQGGDVLTVGYAIYEVPEQFVGQPQVKLKKDYFQYHSSKARSEVFINLREVSQRFTLPAGEYIVVPSTFEPNQEADFILRIFSEKQSVTVSARTTTSNTSPHSPTHTCTYTNTRIPHDGCSHTRERAHSRARTPTPRHSDAPTSFTSFKFSSTCPRFYKLFDRLAGEDQEISAKELQSILNRVVSKLHSTVTTDGFGLEACRSMVSLMDKDGTAKLGVKEFNFLWKKIKNWQQIFLEYDTDSTGTMNSMEMRLALEAAGFRLNEKLNQIVTTRYADGENGITIDFDNYVCCLVRLETMFRTFQALEKDGNEKINLNLPQFLHVTMF 560
BLAST of Calpain vs. Ensembl Nematostella
Match: EDO40193 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7W7]) HSP 1 Score: 544.658 bits (1402), Expect = 0.000e+0 Identity = 304/689 (44.12%), Postives = 425/689 (61.68%), Query Frame = 2
Query: 452 DLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTD---QDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAI--ESAKIEGVMANGLISGHAYSITDVQKVK---QTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMR---QRNLGFLVIGYVIYKLKDP--NCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLE 2479
D+ Q++ TGK +ED +F A SS+F SK + R F WKRP DIC++P +F G SR D+QQG LGDCWLLA +A+L+ + + VVP D D+NY GA +F W++G+W +V++DDRLPTY GKLVF+HS++ NEFWSALLEKAYAK+ G YESL GG EA+EDFTGGL E+ LN K P L+ M K+ LMGC+I ++ +IE + NGL+ GHAY++T ++VK + VELVRVRNPWGNE+EWKG W D S EW+ +S EK+ + F +DGEFWM+FDDF K+FT +EIC LG +S E S +E E G W + + AGGCRNFI +F NPQFR + D+D+++ TVII LMQKD R + + L IG+ IYKL++ G L+ FF++ + A+SP FIN REV GR+ LP G YV+IP+TFNPNEE NF+LR++SEK +KT +D KT A + + +Q + S + ++ F++++G I+A +L+ IL+ L D+ K F LE RS++++ D D +GK+ F++F + W +K W F+ FD D +G D +E R AL G ++SN++ V+ RY + G + +DF+ + RL + Q F D+FLE
Sbjct: 8 DVRRQVRSTGKPFEDDEFLAIDSSIFFSK--RPPRPFEWKRPSDICEDPHMFVGGASRLDIQQGMLGDCWLLAAIAALTQHSALMNKVVPQDYVANPNDRNYTGALKFCIWQYGEWKDVIIDDRLPTYNGKLVFVHSSERNEFWSALLEKAYAKLNGSYESLKGGQTGEALEDFTGGLAESFDLN-KNVPKDLFCIMQTAEKRHALMGCSIAAKANEIEAKLNNGLVKGHAYTVTGCRQVKAKTRRGDLDVELVRVRNPWGNEREWKGAWGDGSSEWQLLSDYEKKSIGLTFEDDGEFWMSFDDFKKHFTTLEICMLGADSAMEGSSVG--FESRSEHGMWRRGLTAGGCRNFIKTFHKNPQFR-ITLQDSDDDADDLCTVIIALMQKDRRKEKKHGINPLTIGFSIYKLEEEKDKQGNLDMEFFQYHASTARSPVFINSREVVGRYALPPGEYVVIPTTFNPNEEGNFVLRMYSEKAHKTCEVD---EKTEFAPQHQRRVQISREQHDTLNS------------KFKEFFDNLSGRDGEIDAKELQQILSTALKNDLGG-KPFSLEGARSIISMYDEDASGKLGFEEFKETWLQVKKWMKIFQVFDEDKSGEMDTYELRGALKEAGFTLSNSVLYAVSARYSTGD-GKVNVDDFMEILTRLNILSAAFQKQAGQGGRRAAFDIDQFLE 673
BLAST of Calpain vs. Ensembl Nematostella
Match: EDO48633 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ83]) HSP 1 Score: 491.5 bits (1264), Expect = 1.855e-163 Identity = 269/677 (39.73%), Postives = 400/677 (59.08%), Query Frame = 2
Query: 419 KEDVGQQTSYFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDK-NYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIE-----SAKIEGVMANGLISGHAYSITDVQKVKQTD---GTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQRNL--GFLVIGYVIYKL-KDPNCG-LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAF 2410
+ + ++ + L QL + +L+ED DFPA +F + S + +WKRP +I K+PQ A G S DV QG + +CW +A +A L+ + +V P DQ F+ +Y G FRF FW+FG+WVE++VDD LPTY G+L F S D +EFWSALLEKAYAK+CG YE++S G EA++D TGG+ E + L P GL+N M+K ++ +LMGC+IE S + EG + NGLI+ HAYSIT ++K + V+L+R+RNP N EW+G WSD SPEW +S EK L F +DGEFWM+FDDF NFT++EIC L P+S+ ++ +R+W++ E G W K + AGGCRNFI SF TNPQFR + D+D + TVI+ LM+ D R IG+ IYK D G L+++FF+++ +A + F RE+ R++L G YV+IPSTFNP +E +F+LRIFSE+P +D + T+ K + DD S + E +F+ MAG ++ +L++IL+ +++ N F LE CRS++++ D D +G + + +F + ++ WK F +D D +G + +E R+AL +G +SN+ + RY + ++G I+F+ FI V+R+ F
Sbjct: 3 RRALHKRERFASLQRQLLNSKRLFEDRDFPASNRVLFTRRRSV---QLIWKRPHEISKSPQFIARGFSSSDVAQGRIRNCWFVAALADLTTNIDLLHHVCPPDQSFEPGSYCGMFRFNFWQFGEWVEIIVDDYLPTYNGRLYFTKSDDPDEFWSALLEKAYAKLCGSYEAMSLGRISEALQDMTGGMIENVDLQNP--PHGLFNVMMKAYERQSLMGCSIEVKKYSSDQYEGTLENGLITRHAYSITGLRKATLSSLRGRPEVDLIRLRNPHANSSEWRGAWSDRSPEWNMLSSQEKRELNLTFDDDGEFWMSFDDFLNNFTRLEICMLTPDSMEQN--DRRKWDVQNEKGRWQKGVTAGGCRNFIASFYTNPQFR-VCLKNPDDDDDELCTVIVSLMRVDHSHRVKIPDRAPIGFAIYKAPSDMRKGDRLDRAFFEYNKAVAMTTSFSKLREISSRYRLHAGEYVVIPSTFNPGQEGDFLLRIFSERPQYASEIDQETKMTSGPKVVITQDDAKS---IDHE--------------FWDVFHQMAGEDGEVDQYELQEILSKSFTRELNN-NLFALEACRSMISMFDTDRSGALGYNEFRALMILLEKWKNHFHKYDRDHSGDMNAYELRDALNGLGYQLSNSAMSSLVLRYHN-KRGTISFDTFIQIVVRVVVMF 652
BLAST of Calpain vs. Ensembl Nematostella
Match: EDO34860 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SN59]) HSP 1 Score: 340.887 bits (873), Expect = 8.598e-106 Identity = 194/504 (38.49%), Postives = 294/504 (58.33%), Query Frame = 2
Query: 446 YFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKN--PQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTD--QDFD----KNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLN------EKERPVGLYNQMLKFAKQSTLMGCAIE---SAKIEGVMANGLISGHAYSITDVQKVKQTDGTV-------VELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGK--RRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGT-VIIGLMQKDMRQ---RNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLD 1867
Y L + + +L+ D F +TSS++ S++ WKRP +IC P LF DG DV QG LG+CW +A +SL+L + V+P Q++D +NY G FRFRF+R G+W EVVVDD LPT GKLV++HS + NEFWSAL+EKAYAK+ G YE+L+ GS +A+ DFTGG+ E I L+ ++E+ + L+ + + K+ +LM +I+ ++E + GLI GHAY +T V+KV +G + ++R+RNPWG EKEW G+WSD S EW+ + +++++ F NDGEFWM FDDF + FT +CH+ +N+ + K ++W + + +W + NAGGC D+F+ NPQ+ ++N G ++ LMQ+D R+ + +G L IG+ I K+++ ++ F K +I FIN REV R L G Y+++PST+ P+ +FM+RIF+ K + LD
Sbjct: 12 YSKLKAECEKNEQLFVDSVFKPETSSLYYSRSCPP-EPVEWKRPSEICHPAVPHLFVDGADSHDVTQGKLGNCWFVAACSSLALRPFLLYKVIPDKDKQEWDPKKPENYQGIFRFRFYRQGEWTEVVVDDLLPTVNGKLVYIHSKEKNEFWSALIEKAYAKLAGTYEALAAGSTADALVDFTGGVAEAISLSNGGYAEDQEKRLQLFKTLERGHKEKSLMSASIKINNRNEMEQRLDTGLIKGHAYGVTMVKKVTVGEGLLSLFNKKHFYMIRLRNPWG-EKEWNGRWSDESEEWKKLKPGDRKKMGIVFENDGEFWMEFDDFCREFTDCTLCHV----INKAMIHKVFKQWHIYKHNYNWTQGQNAGGCVQNQDTFLKNPQY-------AFSITDNEGEDAMVSLMQEDRRKDKSKGVGNLTIGFYIMKVEENRKYRVHSMFPKAGDSI-----FINAREVMNRFFLAAGRYLVVPSTYEPHTPGHFMMRIFTRKRSNAMFLD 497
BLAST of Calpain vs. Ensembl Nematostella
Match: EDO48536 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ84]) HSP 1 Score: 330.872 bits (847), Expect = 2.525e-102 Identity = 189/497 (38.03%), Postives = 262/497 (52.72%), Query Frame = 2
Query: 578 KDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTD----QDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESL--NEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQ---RNLGFLV---------------IGYVIYKLKDPN-------------------------CGLLNKSFFKFSTTIAKSPG-------------------FINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTL 1864
++IC P+ + DG SR D++QG LG+CWLLA ++ L+ ++P+ ++P F Y G F+ W++G+WV V++DDRLPTY GKLVF+HS + NEFW ALLEKAYAK+ G YE+L GG EA+EDFTGGL E L + P L+ M + K+S LMGC+I N +S T G VEL+R+RNPWG+EKEW G W D SPEW+ +S+ EK L F DGEFWM+F+DF +F K+E+C L E+ +S R +E+G W K+++AGGCRNF+++F TNPQFR+ E TG + + + M + +GF + G+ +YK+ + CG + + +K + G FIN REV GR +L G YVI+PSTF N EA+F+LRIFSEK + L
Sbjct: 11 QEICSEPRFYVDGASRLDIKQGVLGNCWLLAAMSVLAQHKPLVDRIMPEGVYEADRFSPRYTGKVVFKLWQYGEWVTVIIDDRLPTYKGKLVFVHSPEKNEFWCALLEKAYAKIHGSYEALKGGLTGEALEDFTGGLSEVFVLEDA--PANLFQIMQRAEKRSALMGCSI----------NVFLS--------------TRGG-VELIRIRNPWGDEKEWTGAWGDRSPEWQELSQDEKRSLDLEFDVDGEFWMSFEDFKSHFDKLEVCMLDVENAIDKTQISFNSR----LENGFWRKHVSAGGCRNFLETFHTNPQFRLVSRVMSRMICEGTGFALYKVSNRVMSREICEGIGFALYKVSNRVISKVLCEGTGFALYKVSNRVISRVICEGTGFALYKVSNRVISRVICGGIGFALYKIDDITKRKYGVSRLDKEYFQFNKSTERSTFINSREVVGRFELAPGEYVIVPSTFIQNAEADFLLRIFSEKAVSSSAL 476
BLAST of Calpain vs. Ensembl Nematostella
Match: EDO37991 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SE78]) HSP 1 Score: 154.066 bits (388), Expect = 5.090e-39 Identity = 102/345 (29.57%), Postives = 168/345 (48.70%), Query Frame = 2
Query: 407 TPKKKEDVGQQTSYFDLLEQLKGTGKLWEDPDFPADTSSVFVS-KASKGYREFVWKRPKDI-----CKN--PQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVG---------LYNQMLKFAKQSTLMG--CAIESAKIE--GVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHL 1378
T +K+++ + +++ K + D FP S++++ + S+ R W RP +I C+ P D+ QG LGDCW L+ +A L+ + + ++ T + + GA++ R + GKW +++DD LP A + W L+EKA AK+ G YE+L+ G IE + TG CE+I L+E + G ++ Q++ LMG C ++ E G A GL + HAYS+ DV+ V + L+R+RNPWG + W G WSD+S W + + L + A +G FWM+ +DF K F +++C +
Sbjct: 17 TRRKRDETHARDQLSRIVDYCKTQEIQFVDDSFPPVLKSLYINPRHSEHPRVADWLRPNEIDNFSTCRTNIPWAVFRNPRPSDIIQGVLGDCWFLSSLAVLAERPELLEKILITREVSPQ---GAYQVRLCKDGKWTVILIDDLLPCNQRGQPVYSWAHRRQLWVPLIEKAMAKLHGSYEALTAGRSIEGLATLTGAPCESIALHESQNNSGNTEPIDKDLIWAQLISSRTAGFLMGASCGGDTQPEEEPGYNAVGLQTHHAYSVLDVRDVHG-----IRLLRLRNPWG-QYSWTGDWSDASSLW---TPELRHELMVHGAQEGVFWMSLEDFMKYFDSVDVCKI 349
BLAST of Calpain vs. Ensembl Medaka
Match: capn3a (calpain-3 [Source:NCBI gene;Acc:101157263]) HSP 1 Score: 512.686 bits (1319), Expect = 1.825e-170 Identity = 291/694 (41.93%), Postives = 426/694 (61.38%), Query Frame = 2
Query: 464 QLKGTGKLWEDPDFPADTSSVF---VSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVK--QTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXN--TGTVIIGLMQKD-MRQRNLG--FLVIGYVIYKLKDPNCG---LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILV--KDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
Q KG L+ED FPA S+ + S + VWKRPK+IC NPQ G SR D+ QG LGDCWLLA +A L+L E + VVP +Q F ++Y G F F+FWR+G WV+VVVDDR+PT +LVF SA+ NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E ++ KE P LY M K ++ +LMGC+I+S A+ E GL+ GHAYS+T V + + Q + V LVR+RNPWG + EW G WSD+S EW T+SK+EKE+L A DGEFWM+F+DF KN+TKIEIC+L P++L +D K W + + +G W + +AGGCRN+ D+F TNPQ+R+ +++ DD+ +N T ++ LMQK+ R+R +G IG+ IY++ G L K FF +++ A+S +IN REV R +L G YVI+PST+ P++E F+LR+FSEK N ++ ++ + +++D PV + + + +F ++AG I A +L+++LN ++ KD+ N GF LE CRS++AL D D TG++ ++F +W IK W+ FK ++ D +G+ + +E RNA+ + G ++N L+ ++ RY + E I F+ FI ++RL F + +D +G + E+L+ +Y
Sbjct: 46 QRKG---LFEDDSFPATVESLGFKELGHKSNKVKNIVWKRPKEICDNPQFIVGGASRTDICQGDLGDCWLLAAIACLTLNEKLLFRVVPQEQSFSESYAGIFHFQFWRYGDWVDVVVDDRIPTLNNQLVFTKSAERNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEM--KEAPKELYKTMKKALERGSLMGCSIDSLVPARFETRTTTGLVKGHAYSVTAVDECRPSQQKESKVRLVRLRNPWG-QVEWNGPWSDNSKEWATLSKAEKEKLQHQSAEDGEFWMSFEDFKKNYTKIEICNLTPDTLEDDKIHK--WTVSVNEGRWLRGCSAGGCRNYPDTFWTNPQYRLRLLEEDDDPDDNEVACTFVVSLMQKNRRRERKMGANLFTIGFSIYEVPKEMHGNKQHLQKDFFLLNSSKARSKSYINLREVTQRFRLSPGEYVIVPSTYEPHQEGEFILRVFSEKRNTSEEIE---------------NRIEADHPVP--APASVGEESEEDHHFRTIFQEIAGEDMEITANKLKNVLNRVITERKDL-NTVGFSLESCRSMIALMDMDGTGRLNLQEFRHLWNKIKQWEGIFKHYNADQSGIINSYEMRNAVNDAGFRLNNQLYHIITLRYAN-ENMNIDFDSFISCLVRLEAMFRAFQAFDQDGDGTIRLSVLEWLQLTMY 712
BLAST of Calpain vs. Ensembl Medaka
Match: capn3a (calpain-3 [Source:NCBI gene;Acc:101157263]) HSP 1 Score: 509.99 bits (1312), Expect = 3.081e-169 Identity = 285/667 (42.73%), Postives = 416/667 (62.37%), Query Frame = 2
Query: 485 LWEDPDFPADTSSVF---VSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXN--TGTVIIGLMQKD-MRQRNLG--FLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILV--KDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEG 2446
L+ED FPA S+ + S + VWKRPK+IC NPQ G SR D+ QG LGDCWLLA +A L+L E + VVP +Q F ++Y G F F+FWR+G WV+VVVDDR+PT +LVF SA+ NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E ++ KE P LY M K ++ +LMGC+I+S A+ E GL+ GHAYS+T V + K++ V LVR+RNPWG + EW G WSD+S EW T+SK+EKE+L A DGEFWM+F+DF KN+TKIEIC+L P++L +D K W + + +G W + +AGGCRN+ D+F TNPQ+R+ +++ DD+ +N T ++ LMQK+ R+R +G IG+ IY++ N L K FF +++ A+S +IN REV R +L G YVI+PST+ P++E F+LR+FSEK N ++ ++ + +++D PV + + + +F ++AG I A +L+++LN ++ KD+ N GF LE CRS++AL D D TG++ ++F +W IK W+ FK ++ D +G+ + +E RNA+ + G ++N L+ ++ RY + E I F+ FI ++RL F + +D +G
Sbjct: 50 LFEDDSFPATVESLGFKELGHKSNKVKNIVWKRPKEICDNPQFIVGGASRTDICQGDLGDCWLLAAIACLTLNEKLLFRVVPQEQSFSESYAGIFHFQFWRYGDWVDVVVDDRIPTLNNQLVFTKSAERNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEM--KEAPKELYKTMKKALERGSLMGCSIDSLVPARFETRTTTGLVKGHAYSVTAVDEQKESK---VRLVRLRNPWG-QVEWNGPWSDNSKEWATLSKAEKEKLQHQSAEDGEFWMSFEDFKKNYTKIEICNLTPDTLEDDKIHK--WTVSVNEGRWLRGCSAGGCRNYPDTFWTNPQYRLRLLEEDDDPDDNEVACTFVVSLMQKNRRRERKMGANLFTIGFSIYEMHG-NKQHLQKDFFLLNSSKARSKSYINLREVTQRFRLSPGEYVIVPSTYEPHQEGEFILRVFSEKRNTSEEIE---------------NRIEADHPVP--APASVGEESEEDHHFRTIFQEIAGEDMEITANKLKNVLNRVITERKDL-NTVGFSLESCRSMIALMDMDGTGRLNLQEFRHLWNKIKQWEGIFKHYNADQSGIINSYEMRNAVNDAGFRLNNQLYHIITLRYAN-ENMNIDFDSFISCLVRLEAMFRAFQAFDQDGDG 688
BLAST of Calpain vs. Ensembl Medaka
Match: capn3a (calpain-3 [Source:NCBI gene;Acc:101157263]) HSP 1 Score: 508.449 bits (1308), Expect = 1.946e-168 Identity = 285/672 (42.41%), Postives = 416/672 (61.90%), Query Frame = 2
Query: 485 LWEDPDFPADTSSVF---VSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES---AKIEGVMANGLISGHAYSITDVQKVK--QTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXN--TGTVIIGLMQKD-MRQRNLG--FLVIGYVIYKLKDPNCG---LLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILV--KDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEG 2446
L+ED FPA S+ + S + VWKRPK+IC NPQ G SR D+ QG LGDCWLLA +A L+L E + VVP +Q F ++Y G F F+FWR+G WV+VVVDDR+PT +LVF SA+ NEFWSALLEKAYAK+ G YE+L GG+ EAMEDFTGG+ E ++ KE P LY M K ++ +LMGC+I+S A+ E GL+ GHAYS+T V + + Q + V LVR+RNPWG + EW G WSD+S EW T+SK+EKE+L A DGEFWM+F+DF KN+TKIEIC+L P++L +D K W + + +G W + +AGGCRN+ D+F TNPQ+R+ +++ DD+ +N T ++ LMQK+ R+R +G IG+ IY++ G L K FF +++ A+S +IN REV R +L G YVI+PST+ P++E F+LR+FSEK N ++ ++ + +++D PV + + + +F ++AG I A +L+++LN ++ KD+ N GF LE CRS++AL D D TG++ ++F +W IK W+ FK ++ D +G+ + +E RNA+ + G ++N L+ ++ RY + E I F+ FI ++RL F + +D +G
Sbjct: 50 LFEDDSFPATVESLGFKELGHKSNKVKNIVWKRPKEICDNPQFIVGGASRTDICQGDLGDCWLLAAIACLTLNEKLLFRVVPQEQSFSESYAGIFHFQFWRYGDWVDVVVDDRIPTLNNQLVFTKSAERNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEM--KEAPKELYKTMKKALERGSLMGCSIDSLVPARFETRTTTGLVKGHAYSVTAVDECRPSQQKESKVRLVRLRNPWG-QVEWNGPWSDNSKEWATLSKAEKEKLQHQSAEDGEFWMSFEDFKKNYTKIEICNLTPDTLEDDKIHK--WTVSVNEGRWLRGCSAGGCRNYPDTFWTNPQYRLRLLEEDDDPDDNEVACTFVVSLMQKNRRRERKMGANLFTIGFSIYEVPKEMHGNKQHLQKDFFLLNSSKARSKSYINLREVTQRFRLSPGEYVIVPSTYEPHQEGEFILRVFSEKRNTSEEIE---------------NRIEADHPVP--APASVGEESEEDHHFRTIFQEIAGEDMEITANKLKNVLNRVITERKDL-NTVGFSLESCRSMIALMDMDGTGRLNLQEFRHLWNKIKQWEGIFKHYNADQSGIINSYEMRNAVNDAGFRLNNQLYHIITLRYAN-ENMNIDFDSFISCLVRLEAMFRAFQAFDQDGDG 697
BLAST of Calpain vs. Ensembl Medaka
Match: capn3b (calpain-3 [Source:NCBI gene;Acc:101169329]) HSP 1 Score: 496.508 bits (1277), Expect = 1.688e-163 Identity = 287/716 (40.08%), Postives = 423/716 (59.08%), Query Frame = 2
Query: 422 EDVGQQTSYFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIE---SAKIEGVMANGLISGHAYSITDVQKVKQT--DGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNT-GTVIIGLMQKDMR-QRNLG--FLVIGYVIYKLKDPNCGL---LNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXX------------XXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVK--DIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLY 2491
+D + ++ +L ++ L+EDP FPA+ SS+F S K + WKRP +IC+NPQ DG +R D+ QG LGDCWLLA +A L+L E + V+P +Q F +NY G F F+FWR+G+W+++VVDDR+PT LVF S NEFWSALLEKAYAK+ G YE+L GG+ ++AMEDFTGG+ E +L+E P GL+ M K ++ +LMGC+I+ + IE GL+ GHAYSI +++ Q DG + L+R+RNPWG WKG WS +S EW T+S ++++ L EFWM+FDDF KNFTK+E+C+L P++L D ++ W + I +G W + +AGGCRNF D+F TNPQ+R+ + + DD + E TV++ LMQK R QR+ G FL IG+ IY++ G L K FF ++ + AK +IN REV R +LP G YVI+P+TF +EE F+LR+FSE NK+ + + + I + +D +++ + E + + EK+ ++ +AG I A +L I+ +L K +IK+ GF LE CRS++AL D D +GK+ ++F +WK IK W+ FK +D DG FE RNA+ + G +++ L+ ++A RY D I F+ +I +RL F +D +G E+L+ +Y
Sbjct: 56 KDAKRLKTFLELRDKYVKKKVLFEDPLFPANDSSLFYSH--KPSMKIEWKRPTEICENPQFIIDGANRTDICQGELGDCWLLAAIACLTLSEKLLYRVIPPEQSFTENYAGIFHFQFWRYGEWIDIVVDDRIPTCNNLLVFTKSFRENEFWSALLEKAYAKLHGSYEALKGGNTLDAMEDFTGGVTELFELSEA--PKGLFIIMKKALERGSLMGCSIDVTTANDIESRTNQGLVRGHAYSIIALEECDQVSEDGK-IRLIRLRNPWG-WVLWKGPWSVNSKEWSTVSTADRDNLKKQTLEMSEFWMSFDDFKKNFTKLEMCNLTPDALTGD--ERQSWTVSINEGRWVRGSSAGGCRNFPDTFWTNPQYRLRLYEEDDPEEEQVLCTVVVALMQKGRRMQRHAGAKFLTIGFSIYEVPKELQGQNQHLQKDFFLYNASKAKCKAYINLREVTERFRLPPGEYVILPTTFKAHEEGEFLLRVFSE--NKSTSEEAETSIDKIEPIVFVSDRARTNKEIEHEGIRGEKKKKPKRKPLQAVEETEEEKQFRVIYQKIAGEDMEICASELMAIMKNVLSKHSEIKS-DGFSLETCRSMIALMDTDGSGKLNLQEFKHLWKKIKEWQLIFKRYDKDGKCSISSFEMRNAVNDAGFNLNKQLYDIIAMRYADKHLN-IDFDSYICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMY 759
BLAST of Calpain vs. Ensembl Medaka
Match: capn9 (calpain 9 [Source:NCBI gene;Acc:101158662]) HSP 1 Score: 489.96 bits (1260), Expect = 2.315e-162 Identity = 279/696 (40.09%), Postives = 409/696 (58.76%), Query Frame = 2
Query: 392 VQAKHTPKKKEDVGQQTSYFD--LLEQLKG----TGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIE---SAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKD---MRQRNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVA------LEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMEL 2425
+Q+ + KK V +++ +D EQL+ G L+EDPDFPA SS+F S+ + WKRPK+IC+NP+ G R D+ QG LGDCWLLA +ASL+L + VVP DQDFD NY G F F+FW+ KW++VVVDDRLPT +L+ +HSA N+EFWSALLEKAYAK+ G YESL GGS +EAMEDFTGG+ E + K P L++ M K + ++MGC+I+ SA+ E + GL+ GHAYSIT ++++ Q G V+L+RVRNPWG + EW G WSD+S EW I K EK+R+ N DGEFWM F+DF KN+ K+EIC++ P++L++D KR W + + +G+W + AGGCRN+ID+F TNPQFR ++ D+D ++ +V+I LMQK+ +R+ L IG+ +Y+ D + K FF++ + A+S +IN REV R LP G Y+++P+T+ P++EA+F++RIFSEK +T+ K LR +LF+ +AG + N +NF + +L++ D+TG +EF++F W+ +K W F FD D +G +E R+AL G+ ++N L ++V R+ D + I F+D++ ++RL N F+ E+
Sbjct: 3 LQSTLSGKKSSSVQAESTQWDGKTFEQLRQECLQKGVLFEDPDFPAVDSSLFYSQTVP--VQIQWKRPKEICENPKFIVGGADRTDICQGQLGDCWLLAAIASLTLKKDAMARVVPPDQDFDHNYAGIFHFQFWQHNKWLDVVVDDRLPTVRNRLIMLHSASNDEFWSALLEKAYAKLNGSYESLKGGSTMEAMEDFTGGVGEMYET--KSAPENLFSIMKKALDRGSMMGCSIDITSSAESEAKTSTGLVKGHAYSITGMEEL-QFRGQTVKLIRVRNPWG-QVEWNGAWSDNSREWNYIDKKEKDRILQNSLEDGEFWMEFEDFKKNYDKVEICNMTPDALDDDT--KRHWAVNVFEGNWIRGSTAGGCRNYIDTFWTNPQFR-LQLEDADDDDDDVCSVVIALMQKNRRKLRKEGLDLETIGFAVYEAPDDESQ-VEKDFFRYHPSKARSRTYINMREVSERFTLPPGKYLLVPTTYQPHQEADFIIRIFSEKKADPPMPTPPEEETDEEKSLR------------------------------RLFDQLAGDVREQN----------------QNFPPTRISYEANLLSGCFPERRNQVDNTGMIEFQEFKVFWEKMKNWIMLFLAFDTDRSGKMSSYELRSALKAAGMQLNNQLLQLVGLRFGD-DTFNIDFDDYLTCIVRLENMFKLTEI 641
BLAST of Calpain vs. Planmine SMEST
Match: SMESG000062902.1 (SMESG000062902.1) HSP 1 Score: 1376.3 bits (3561), Expect = 0.000e+0 Identity = 697/703 (99.15%), Postives = 699/703 (99.43%), Query Frame = 2
Query: 386 SKVQAKHTPKKKEDVGQQTSYFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQRNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLYM 2494
SKVQAK TPKKKEDVGQQTSY DLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREF WKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRVEVVDPDDEDSENTGTVIIGLMQKDMRQRNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTT+AKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESKVKNEKLVALEKELEKLFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDH+GKMEFKDFMKIWKDIKVWKTAFKDFD DGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLYM
Sbjct: 32 SKVQAKQTPKKKEDVGQQTSYSDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFEWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRVEVVDPDDEDSENTGTVIIGLMQKDMRQRNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTVAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESKVKNEKLVALEKELEKLFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHSGKMEFKDFMKIWKDIKVWKTAFKDFDSDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLYM 734
BLAST of Calpain vs. Planmine SMEST
Match: SMESG000062904.1 (SMESG000062904.1) HSP 1 Score: 1258.05 bits (3254), Expect = 0.000e+0 Identity = 628/700 (89.71%), Postives = 664/700 (94.86%), Query Frame = 2
Query: 395 QAKHTPKKKEDVGQQTSYFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQRNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLYM 2494
Q K TP KK D+ +QTSY D+LE LKGTGKLWEDPDFPADTSSVFVSKASKGYREF WKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTY G+LVFMHSADNNEFWSALLEKAYAKMCGIYESL+GGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRVEVVDPDDEDSE+TGTVIIGLMQKDMRQRNL FLVIGYVIYKLKDPNCGLLNKSFFKFSTT+AKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEK NK+ LD++NGKT IAKHLR+TD VD + VSEE KVKNEK++ALEK+LE+LF MAGPMEVINACQLRDILNA+L KD+KNFKGFDLE+CRS++AL+D DHTGKMEF +F+ +W ++++WKTAFKDFD D NGVFD +EFRNAL NVGL+VSN LFKV+ NRY DPE GVI FEDFIMAVIRLTNAFETMELQP+D+E NYVFTA+EF+ESFLYM
Sbjct: 35 QLKPTPMKKRDLDKQTSYSDILEHLKGTGKLWEDPDFPADTSSVFVSKASKGYREFEWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYRGRLVFMHSADNNEFWSALLEKAYAKMCGIYESLNGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRVEVVDPDDEDSEDTGTVIIGLMQKDMRQRNLDFLVIGYVIYKLKDPNCGLLNKSFFKFSTTVAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKQNKSKELDVENGKTEIAKHLRNTDMVDDGRAVSEEVKVKNEKILALEKDLEELFKVMAGPMEVINACQLRDILNAVLKKDMKNFKGFDLEVCRSMIALKDDDHTGKMEFPEFLLLWTNLRIWKTAFKDFDADENGVFDAYEFRNALNNVGLTVSNTLFKVIGNRYTDPENGVIKFEDFIMAVIRLTNAFETMELQPQDEEKNYVFTAEEFMESFLYM 734
BLAST of Calpain vs. Planmine SMEST
Match: SMESG000062904.1 (SMESG000062904.1) HSP 1 Score: 1246.49 bits (3224), Expect = 0.000e+0 Identity = 619/700 (88.43%), Postives = 657/700 (93.86%), Query Frame = 2
Query: 395 QAKHTPKKKEDVGQQTSYFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQRNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLYM 2494
Q K TP KK D+ +QTSY D+LE LKGTGKLWEDPDFPADTSSVFVSKASKGYREF WKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTY G+LVFMHSADNNEFWSALLEKAYAKMCGIYESL+GGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRVEVVDPDDEDSE+TGTVIIGLMQKDMRQRNL FLVIGYVIYKLKDPNCGLLNKSFFKFSTT+AKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEK NK+ LD++NGKT IAKHLR+TD VD + +E+ ++ALEK+LE+LF MAGPMEVINACQLRDILNA+L KD+KNFKGFDLE+CRS++AL+D DHTGKMEF +F+ +W ++++WKTAFKDFD D NGVFD +EFRNAL NVGL+VSN LFKV+ NRY DPE GVI FEDFIMAVIRLTNAFETMELQP+D+E NYVFTA+EF+ESFLYM
Sbjct: 35 QLKPTPMKKRDLDKQTSYSDILEHLKGTGKLWEDPDFPADTSSVFVSKASKGYREFEWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYRGRLVFMHSADNNEFWSALLEKAYAKMCGIYESLNGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRVEVVDPDDEDSEDTGTVIIGLMQKDMRQRNLDFLVIGYVIYKLKDPNCGLLNKSFFKFSTTVAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKQNKSKELDVENGKTEIAKHLRNTDMVDEVKVKNEK-------ILALEKDLEELFKVMAGPMEVINACQLRDILNAVLKKDMKNFKGFDLEVCRSMIALKDDDHTGKMEFPEFLLLWTNLRIWKTAFKDFDADENGVFDAYEFRNALNNVGLTVSNTLFKVIGNRYTDPENGVIKFEDFIMAVIRLTNAFETMELQPQDEEKNYVFTAEEFMESFLYM 727
BLAST of Calpain vs. Planmine SMEST
Match: SMESG000062904.1 (SMESG000062904.1) HSP 1 Score: 1235.32 bits (3195), Expect = 0.000e+0 Identity = 620/700 (88.57%), Postives = 654/700 (93.43%), Query Frame = 2
Query: 395 QAKHTPKKKEDVGQQTSYFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQRNLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNGKTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLYM 2494
Q K TP KK D+ +QTSY D+LE LKGTGKLWEDPDFPADTSSVFVSKASKGYREF WKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTY G+LVFMHSADNNEFWSALLEKAYAKMCGIYESL+GGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRVEVVDPDDEDSE+TGTVIIGLMQKDMRQRNL FLVIGYVIYKLKDPNCGLLNKSFFKFSTT+AKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEK NK+ LD++NGKT IAKHL E KVKNEK++ALEK+LE+LF MAGPMEVINACQLRDILNA+L KD+KNFKGFDLE+CRS++AL+D DHTGKMEF +F+ +W ++++WKTAFKDFD D NGVFD +EFRNAL NVGL+VSN LFKV+ NRY DPE GVI FEDFIMAVIRLTNAFETMELQP+D+E NYVFTA+EF+ESFLYM
Sbjct: 35 QLKPTPMKKRDLDKQTSYSDILEHLKGTGKLWEDPDFPADTSSVFVSKASKGYREFEWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDKNYIGAFRFRFWRFGKWVEVVVDDRLPTYRGRLVFMHSADNNEFWSALLEKAYAKMCGIYESLNGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIESAKIEGVMANGLISGHAYSITDVQKVKQTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNEDLSGKRRWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRVEVVDPDDEDSEDTGTVIIGLMQKDMRQRNLDFLVIGYVIYKLKDPNCGLLNKSFFKFSTTVAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKQNKSKELDVENGKTEIAKHL--------------EVKVKNEKILALEKDLEELFKVMAGPMEVINACQLRDILNAVLKKDMKNFKGFDLEVCRSMIALKDDDHTGKMEFPEFLLLWTNLRIWKTAFKDFDADENGVFDAYEFRNALNNVGLTVSNTLFKVIGNRYTDPENGVIKFEDFIMAVIRLTNAFETMELQPQDEEKNYVFTAEEFMESFLYM 720
BLAST of Calpain vs. Planmine SMEST
Match: SMESG000039299.1 (SMESG000039299.1) HSP 1 Score: 688.723 bits (1776), Expect = 0.000e+0 Identity = 361/703 (51.35%), Postives = 478/703 (67.99%), Query Frame = 2
Query: 428 VGQQTSYFDLLEQLKGTGKLWEDPDFPADTSSVFVSKASKGYREFVWKRPKDICKNPQLFADGVSRFDVQQGALGDCWLLAVVASLSLYEPIFKNVVPTDQDFDK-NYIGAFRFRFWRFGKWVEVVVDDRLPTYGGKLVFMHSADNNEFWSALLEKAYAKMCGIYESLSGGSQIEAMEDFTGGLCETIKLNEKERPVGLYNQMLKFAKQSTLMGCAIES--AKIEGVMANGLISGHAYSITDVQKVK---QTDGTVVELVRVRNPWGNEKEWKGKWSDSSPEWETISKSEKERLCSNFANDGEFWMTFDDFAKNFTKIEICHLGPESLNED-LSGKR--RWEMCIEDGSWCKNINAGGCRNFIDSFVTNPQFRXXXXXXXXXXXXNTGTVIIGLMQKDMRQR---NLGFLVIGYVIYKLKDPNCGLLNKSFFKFSTTIAKSPGFINQREVCGRHKLPTGHYVIIPSTFNPNEEANFMLRIFSEKPNKTDTLDIKNG--KTNIAKHLRDTDDVDSDQPVSEESXXXXXXXXXXXXXXXXXFNDMAGPMEVINACQLRDILNAILVKDIKNFKGFDLELCRSLVALEDYDHTGKMEFKDFMKIWKDIKVWKTAFKDFDCDGNGVFDCFEFRNALINVGLSVSNALFKVVANRYIDPEKGVITFEDFIMAVIRLTNAFETMELQPEDDEGNYVFTADEFLESFLYM 2494
VG Y ++ L+G KL+EDPDFPA S++ S+ + R+ WKRP +IC+NP+ ++G SRFDV+QG LGDCWLLA VA LSL+ +F VVP+DQ+F K +Y G FRFRFWRFG W EV+VDDRLPTY KLV+MHS D+NEFWSALLEKAYAK+ G YESL GGS EA+EDFTGG+ E + +KE P L++ M K + +LMGC+IE+ K E + NGLI GHAYSITDV+ V ++L+R+RNPWGNE EWKG WSD SPEW IS++++ + F +DGEFWM++ DF KNF K+EICHLGP+SL D L GK+ RWEMCIE+G W K +NAGGCRNF+D+F TNPQFRV+VVDPD++D EN GT+IIGLMQKDMR+R + F IGY IY+LKD N G L+ ++FK++ ++AK+P FIN REVCGRHKLP G YVI+PSTF PN+EA+F++RIFSEKPN++ LD + T K + DD L + FN +AG I+A +L+DILN KD K F GF +E +S+VA+ DYD +GK+ F +F +W D+++WK AFK FD D +G F+ +E R L +VG +SN + + R+ + E G++ F+D+I+ +RL FET + QP D + +F D F++ +Y+
Sbjct: 95 VGPSQDYNNVKNSLRG--KLYEDPDFPAVNESLYFSQ--RPPRKIDWKRPHEICQNPEFISEGASRFDVRQGELGDCWLLAAVACLSLHSELFNQVVPSDQNFSKPSYCGMFRFRFWRFGAWKEVLVDDRLPTYNNKLVYMHSTDSNEFWSALLEKAYAKISGSYESLKGGSTSEALEDFTGGVTEFFDM-KKEVPKNLFDIMFKAFSRCSLMGCSIEADPRKTEAQLPNGLIMGHAYSITDVKMVNINIPGKSGKIQLIRIRNPWGNEAEWKGSWSDKSPEWNYISQADRNTMGLTFDDDGEFWMSYQDFIKNFEKMEICHLGPQSLEADQLGGKKKSRWEMCIENGEWKKRLNAGGCRNFLDTFWTNPQFRVKVVDPDEDDDENLGTIIIGLMQKDMRKRRKEGVEFYTIGYAIYQLKDENQGPLDINYFKYNGSVAKAPSFINLREVCGRHKLPPGTYVIVPSTFEPNQEASFLMRIFSEKPNESGELDEQTAMIPTEPEKVVSPADDAQ-------------------IMALRQSFNQIAGQDLEIDAYELQDILNTAFKKDFK-FDGFSIETSKSMVAMMDYDQSGKLGFDEFKVLWTDLRLWKEAFKKFDQDKSGNFNSYELRLCLNSVGFRLSNKILNSLVMRFANKE-GLVLFDDYILCCVRLKTIFETYKSQPHDSKDRAMFDQDTFIQCIMYV 771 The following BLAST results are available for this feature:
BLAST of Calpain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Calpain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Calpain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Calpain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Calpain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Calpain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Calpain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Calpain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Calpain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Calpain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Calpain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Calpain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Calpain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Calpain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30013312 ID=SMED30013312|Name=Calpain|organism=Schmidtea mediterranea sexual|type=transcript|length=2680bpback to top protein sequence of SMED30013312-orf-1 >SMED30013312-orf-1 ID=SMED30013312-orf-1|Name=SMED30013312-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=735bp MSSSKSVTTKVTSTKTTRIGGGTTTTTTKTASKVQAKHTPKKKEDVGQQTback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
