SMED30013281
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30013281 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Alignments
SMED30013281 aligns in the following genomic locations:
Homology
BLAST of SMED30013281 vs. Planmine SMEST
Match: SMESG000025524.1 (SMESG000025524.1) HSP 1 Score: 167.162 bits (422), Expect = 2.359e-52 Identity = 103/157 (65.61%), Postives = 119/157 (75.80%), Query Frame = -2 Query: 1390 TGSTLNEKVNKRKSMLNYIMILREASAIEFVTINAIEEDIEIIGQNLNPILGTMERGHIXXXXXXXXXXXXKFDKEIIDVNKFESNKNILLKRPINSENAFPKMIKALLKRGKIKFTDNKRESDRIGFRKEELQTLVGESHRIHQISSGIKKEIDNI 1860 TGSTLNEK NK K+MLNYIM+ REA AI+FVTINAI++DIEIIGQNLNPIL +ERG+ +AA K+IKW+KRK D EIIDVNKFESN+NIL+KR + SE A PK IKAL E +RI RKEEL TLVGE HRI SG K+EID+I Sbjct: 536 TGSTLNEKDNKGKTMLNYIMMSREARAIDFVTINAIQDDIEIIGQNLNPILEAIERGYEEAAEKLIKWRKRKSDNEIIDVNKFESNENILVKRAMKSEYALPKTIKAL-------------EPERIVSRKEELDTLVGEWHRIQGKPSGKKREIDDI 679 HSP 2 Score: 58.9214 bits (141), Expect = 2.359e-52 Identity = 49/87 (56.32%), Postives = 56/87 (64.37%), Query Frame = -1 Query: 1076 NVEDNSIEKARCXXXXXXXXXXXXXXXXXXXXXXXXXXDINKNQDSVTYKEIRQQPKSKNDDYQKIPRDEENTDKNYWKQMIDSQKF 1336 N EDN IEKARC+K QDKK+D++ + D N+ K Q SV YKEI QQ K N D QK +DEENTDK WKQMIDSQK Sbjct: 698 NAEDNRIEKARCNKNQDKKEDDNEEGENRDRNK--------KKQISVAYKEIGQQSKPNNVDNQKRSKDEENTDKICWKQMIDSQKI 776
BLAST of SMED30013281 vs. Planmine SMEST
Match: SMESG000050768.1 (SMESG000050768.1) HSP 1 Score: 157.918 bits (398), Expect = 1.333e-48 Identity = 100/158 (63.29%), Postives = 117/158 (74.05%), Query Frame = -2 Query: 1390 TGSTLNEKVNKRKSMLNYIMILREASAIEFVTINAIEEDIEIIGQNLNPILGTMERGHIXXXXXXXXXXXXKFDKEIIDVNKFESNKNILLKRPINSENAFPKMIKALLKRGKIKFTDNKRESDRIGFRKEELQTLVGESHRIHQISSGIKK-EIDNI 1860 TGSTLNE NK K+MLNY+M+ REA AI+ VTIN I+EDIE+IGQNLNPILG + RG+ +AAAK+I W+KRK D EIIDVNKFESN+NIL+KR + SE A PK IKAL E +RI RKEEL+TLVGE HRI SG KK EID+I Sbjct: 276 TGSTLNENDNKGKTMLNYVMMSREARAIDLVTINGIKEDIEVIGQNLNPILGAIGRGYDEAAAKLITWRKRKSDNEIIDVNKFESNENILVKRAMKSEYALPKTIKAL-------------EPERIMPRKEELETLVGEWHRIQGKPSGKKKQEIDDI 420 HSP 2 Score: 55.4546 bits (132), Expect = 1.333e-48 Identity = 47/87 (54.02%), Postives = 58/87 (66.67%), Query Frame = -1 Query: 1076 NVEDNSIEKARCXXXXXXXXXXXXXXXXXXXXXXXXXXDINKNQDSVTYKEIRQQPKSKNDDYQKIPRDEENTDKNYWKQMIDSQKF 1336 N EDN IEKA+C+K QDKK+D++ +R D N+ + Q+ V YKEIRQQ K N D QK +DEENTDK WKQM+DSQK Sbjct: 439 NAEDNRIEKAQCNKNQDKKEDDNEERENRDRNK--------ERQNPVAYKEIRQQSKPNNIDDQKRSKDEENTDKICWKQMMDSQKI 517
BLAST of SMED30013281 vs. Planmine SMEST
Match: SMESG000028578.1 (SMESG000028578.1) HSP 1 Score: 149.828 bits (377), Expect = 2.280e-47 Identity = 96/157 (61.15%), Postives = 116/157 (73.89%), Query Frame = -2 Query: 1390 TGSTLNEKVNKRKSMLNYIMILREASAIEFVTINAIEEDIEIIGQNLNPILGTMERGHIXXXXXXXXXXXXKFDKEIIDVNKFESNKNILLKRPINSENAFPKMIKALLKRGKIKFTDNKRESDRIGFRKEELQTLVGESHRIHQISSGIKKEIDNI 1860 TGSTLNEK NK K+MLNYIM+ E AI+FVTIN I++ I+IIGQNLNPIL +ERG+ +AAAK+IKW+KRK D EIIDVNKFESN+N+L+KR + S+ A PK IKAL E +RI RKEEL+TLV E HRI SG K+EID+I Sbjct: 293 TGSTLNEKDNKGKTMLNYIMMSLETRAIDFVTINGIQDYIQIIGQNLNPILEAIERGYDEAAAKLIKWRKRKSDNEIIDVNKFESNENVLVKRAMKSKYALPKTIKAL-------------EPERIVSRKEELETLVVEWHRIQGKPSGKKQEIDDI 436 HSP 2 Score: 59.6918 bits (143), Expect = 2.280e-47 Identity = 49/88 (55.68%), Postives = 56/88 (63.64%), Query Frame = -1 Query: 1073 NVEDNSIEKARCXXXXXXXXXXXXXXXXXXXXXXXXXXDINKNQDSVTYKEIRQQPKSKNDDYQKIPRDEENTDKNYWKQMIDSQKFR 1336 N EDN IEKARC+K QD K+DE+ + D N+ K Q SV YKEI QQ K N D QK +DEENTDK WKQMIDSQK + Sbjct: 455 NAEDNRIEKARCNKNQDNKEDENEEGENRDRNK--------KKQISVAYKEIGQQSKPNNVDNQKRSKDEENTDKICWKQMIDSQKIQ 534
BLAST of SMED30013281 vs. Planmine SMEST
Match: SMESG000027747.1 (SMESG000027747.1) HSP 1 Score: 120.168 bits (300), Expect = 5.553e-47 Identity = 82/123 (66.67%), Postives = 98/123 (79.67%), Query Frame = -1 Query: 311 KEREKYCKDQKKDEGNLKDNEXXXXXXXXXXXXXXXXXXXXKLRKAKTKNKP*RLDDLRKEVLIIVVADSRKYETT*KLYEKLITKIFRISRNSRRKVNGIEIKEEKNMAPRLLTRNNTESIY 679 +++EKYCK+QK DE NLKDNET T +RTT +SAKSTQSS+KLRK T NKP R+DD+R EV I VV D+ KYET K+ KL TK+ +I +NS+RKVNG EIKEEKNM PRLL RNNT+SIY Sbjct: 292 QKKEKYCKNQKSDEENLKDNETRKTISRTTNESAKSTQSSTKLRKTTTPNKPQRMDDVRNEVSISVVTDAWKYETIRKVQGKLFTKLSKIVKNSKRKVNGREIKEEKNMTPRLL-RNNTKSIY 413 HSP 2 Score: 72.0182 bits (175), Expect = 5.553e-47 Identity = 38/51 (74.51%), Postives = 42/51 (82.35%), Query Frame = -3 Query: 174 YRIYI*QGLSRNFPQRGVRNNKGKTTKESATRNQADSRKVISKDDRHSSEK 326 Y+I+ QGLSRN QRGVRNNKG+TTKES TRNQ DSRKVISK + H SEK Sbjct: 413 YQIW--QGLSRNSSQRGVRNNKGETTKESPTRNQTDSRKVISKYNGHLSEK 461 HSP 3 Score: 29.261 bits (64), Expect = 5.553e-47 Identity = 24/72 (33.33%), Postives = 33/72 (45.83%), Query Frame = -2 Query: 25 LIRKGKNHLLAVIKIV*N-----HKTSRIXXXXXXXXXXXXXKS-------------QMRVTGKYKRKPMGK 186 L K NHLL + KIV N KT++++ T + + Q+R TGKYK+KPM K Sbjct: 458 LSEKETNHLLEIFKIVQNLENPSKKTTKLENTTSITVKLVAISEIVTNVIVMWMIIRQVRATGKYKQKPMKK 529 HSP 4 Score: 27.335 bits (59), Expect = 5.553e-47 Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -1 Query: 2 KRMWPKPEN 28 KRMWPKPEN Sbjct: 529 KRMWPKPEN 537 HSP 5 Score: 164.851 bits (416), Expect = 1.339e-43 Identity = 93/157 (59.24%), Postives = 110/157 (70.06%), Query Frame = -2 Query: 1390 TGSTLNEKVNKRKSMLNYIMILREASAIEFVTINAIEEDIEIIGQNLNPILGTMERGHIXXXXXXXXXXXXKFDKEIIDVNKFESNKNILLKRPINSENAFPKMIKALLKRGKIKFTDNKRESDRIGFRKEELQTLVGESHRIHQISSGIKKEIDNI 1860 TGSTLNEK NK K+MLNYIM+LREASAIEF+TINAI++DIEIIGQNLNPIL +ER ++D+ L + INSE A PK IKALLKR +IKFTDNKRE +RIG RKEEL+TLV E HRI + SSG K+E+D+I Sbjct: 115 TGSTLNEKDNKGKTMLNYIMMLREASAIEFITINAIQDDIEIIGQNLNPILEAIER---------------EYDEAAAK-----------LIKAINSEYALPKTIKALLKRREIKFTDNKREHERIGSRKEELETLVEEWHRIQRKSSGRKQEVDDI 245
BLAST of SMED30013281 vs. Planmine SMEST
Match: SMESG000027747.1 (SMESG000027747.1) HSP 1 Score: 120.168 bits (300), Expect = 6.752e-47 Identity = 82/123 (66.67%), Postives = 98/123 (79.67%), Query Frame = -1 Query: 311 KEREKYCKDQKKDEGNLKDNEXXXXXXXXXXXXXXXXXXXXKLRKAKTKNKP*RLDDLRKEVLIIVVADSRKYETT*KLYEKLITKIFRISRNSRRKVNGIEIKEEKNMAPRLLTRNNTESIY 679 +++EKYCK+QK DE NLKDNET T +RTT +SAKSTQSS+KLRK T NKP R+DD+R EV I VV D+ KYET K+ KL TK+ +I +NS+RKVNG EIKEEKNM PRLL RNNT+SIY Sbjct: 298 QKKEKYCKNQKSDEENLKDNETRKTISRTTNESAKSTQSSTKLRKTTTPNKPQRMDDVRNEVSISVVTDAWKYETIRKVQGKLFTKLSKIVKNSKRKVNGREIKEEKNMTPRLL-RNNTKSIY 419 HSP 2 Score: 72.0182 bits (175), Expect = 6.752e-47 Identity = 38/51 (74.51%), Postives = 42/51 (82.35%), Query Frame = -3 Query: 174 YRIYI*QGLSRNFPQRGVRNNKGKTTKESATRNQADSRKVISKDDRHSSEK 326 Y+I+ QGLSRN QRGVRNNKG+TTKES TRNQ DSRKVISK + H SEK Sbjct: 419 YQIW--QGLSRNSSQRGVRNNKGETTKESPTRNQTDSRKVISKYNGHLSEK 467 HSP 3 Score: 28.8758 bits (63), Expect = 6.752e-47 Identity = 24/72 (33.33%), Postives = 33/72 (45.83%), Query Frame = -2 Query: 25 LIRKGKNHLLAVIKIV*N-----HKTSRIXXXXXXXXXXXXXKS-------------QMRVTGKYKRKPMGK 186 L K NHLL + KIV N KT++++ T + + Q+R TGKYK+KPM K Sbjct: 464 LSEKETNHLLEIFKIVQNLENPSKKTTKLENTTSITVKLVAISEIVTNVIVMWMIIRQVRATGKYKQKPMKK 535 HSP 4 Score: 27.335 bits (59), Expect = 6.752e-47 Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -1 Query: 2 KRMWPKPEN 28 KRMWPKPEN Sbjct: 535 KRMWPKPEN 543 HSP 5 Score: 164.851 bits (416), Expect = 1.681e-43 Identity = 93/157 (59.24%), Postives = 110/157 (70.06%), Query Frame = -2 Query: 1390 TGSTLNEKVNKRKSMLNYIMILREASAIEFVTINAIEEDIEIIGQNLNPILGTMERGHIXXXXXXXXXXXXKFDKEIIDVNKFESNKNILLKRPINSENAFPKMIKALLKRGKIKFTDNKRESDRIGFRKEELQTLVGESHRIHQISSGIKKEIDNI 1860 TGSTLNEK NK K+MLNYIM+LREASAIEF+TINAI++DIEIIGQNLNPIL +ER ++D+ L + INSE A PK IKALLKR +IKFTDNKRE +RIG RKEEL+TLV E HRI + SSG K+E+D+I Sbjct: 121 TGSTLNEKDNKGKTMLNYIMMLREASAIEFITINAIQDDIEIIGQNLNPILEAIER---------------EYDEAAAK-----------LIKAINSEYALPKTIKALLKRREIKFTDNKREHERIGSRKEELETLVEEWHRIQRKSSGRKQEVDDI 251 The following BLAST results are available for this feature:
BLAST of SMED30013281 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30013281 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30013281 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30013281 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30013281 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30013281 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30013281 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30013281 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30013281 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30013281 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30013281 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30013281 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30013281 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30013281 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30013281 ID=SMED30013281|Name=SMED30013281|organism=Schmidtea mediterranea sexual|type=transcript|length=1861bpback to top protein sequence of SMED30013281-orf-1 >SMED30013281-orf-1 ID=SMED30013281-orf-1|Name=SMED30013281-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=163bp TGSTLNEKVNKRKSMLNYIMILREASAIEFVTINAIEEDIEIIGQNLNPIback to top protein sequence of SMED30013281-orf-2 >SMED30013281-orf-2 ID=SMED30013281-orf-2|Name=SMED30013281-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=133bp MMIKMYKEIIKEINVEDNSIEKARCDKGQDKKDDEDNDRNKEDDNEEEANback to top InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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