Pedal peptide prohormone like-2

Overview
NamePedal peptide prohormone like-2
Smed IDSMED30013280
Length (bp)351
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Pedal peptide prohormone like-2 (SMED30013280) t-SNE clustered cells

Violin plots show distribution of expression levels for Pedal peptide prohormone like-2 (SMED30013280) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013280

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30013280SMESG000059588.1 SmedASXL_004971SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30013280SMESG000059588.1 dd_Smed_v4_1520_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30013280SMESG000059588.1 dd_Smed_v4_1520_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30013280SMESG000059588.1 dd_Smed_v4_1520_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30013280SMESG000059588.1 dd_Smed_v6_1520_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30013280SMESG000059588.1 BK007018smed_ncbi_20200123PMID:20967238
Collins JJ et al., 2010		whole organism asexual adult colorimetric in situ hybridization evidence
cephalic gangliaSMED30013280SMESG000059588.1 BK007018smed_ncbi_20200123PMID:20967238
Collins JJ et al., 2010		whole organism asexual adult colorimetric in situ hybridization evidence
ventral nerve cordSMED30013280SMESG000059588.1 BK007018smed_ncbi_20200123PMID:20967238
Collins JJ et al., 2010		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Pedal peptide prohormone like-2 vs. Ensembl Xenopus
Match: ENSXETT00000023923.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:10:33449893:33451241:1 gene:ENSXETG00000015755.1 transcript:ENSXETT00000023923.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 45.8246 bits (107), Expect = 3.000e-6
Identity = 27/64 (42.19%), Postives = 32/64 (50.00%), Query Frame = -2 
Query:  102 NKSLPILSIYDRFSISLPILSKYRRFSRSLPILSIYDRFSKSFPILSKYDRLRRSLPILSKYRN 293
             + LPIL  Y      LPIL  Y+   R LPIL  Y    +  PIL  Y  L+R LPIL  Y+ 
Sbjct:  154 QRLLPILLSYQALQRLLPILLSYQGLQRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQG 217

HSP 2 Score: 45.0542 bits (105), Expect = 4.921e-6
Identity = 27/64 (42.19%), Postives = 32/64 (50.00%), Query Frame = -2 
Query:  102 NKSLPILSIYDRFSISLPILSKYRRFSRSLPILSIYDRFSKSFPILSKYDRLRRSLPILSKYRN 293
             + LPIL  Y      LPIL  Y+   R LPIL  Y    +  PIL  Y  L+R LPIL  Y+ 
Sbjct:  219 QRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQA 282

HSP 3 Score: 45.0542 bits (105), Expect = 4.921e-6
Identity = 27/64 (42.19%), Postives = 32/64 (50.00%), Query Frame = -2 
Query:  102 NKSLPILSIYDRFSISLPILSKYRRFSRSLPILSIYDRFSKSFPILSKYDRLRRSLPILSKYRN 293
             + LPIL  Y      LPIL  Y+   R LPIL  Y    +  PIL  Y  L+R LPIL  Y+ 
Sbjct:  232 QRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQA 295

HSP 4 Score: 45.0542 bits (105), Expect = 4.921e-6
Identity = 27/64 (42.19%), Postives = 32/64 (50.00%), Query Frame = -2 
Query:  102 NKSLPILSIYDRFSISLPILSKYRRFSRSLPILSIYDRFSKSFPILSKYDRLRRSLPILSKYRN 293
             + LPIL  Y      LPIL  Y+   R LPIL  Y    +  PIL  Y  L+R LPIL  Y+ 
Sbjct:  245 QRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQA 308

HSP 5 Score: 45.0542 bits (105), Expect = 5.529e-6
Identity = 27/64 (42.19%), Postives = 32/64 (50.00%), Query Frame = -2 
Query:  102 NKSLPILSIYDRFSISLPILSKYRRFSRSLPILSIYDRFSKSFPILSKYDRLRRSLPILSKYRN 293
             + LPIL  Y      LPIL  Y+   R LPIL  Y    +  PIL  Y  L+R LPIL  Y+ 
Sbjct:  115 QRLLPILLSYKALQRLLPILLSYKALQRLLPILLSYKALQRLLPILLSYQALQRLLPILLSYQG 178

HSP 6 Score: 45.0542 bits (105), Expect = 5.529e-6
Identity = 27/64 (42.19%), Postives = 32/64 (50.00%), Query Frame = -2 
Query:  102 NKSLPILSIYDRFSISLPILSKYRRFSRSLPILSIYDRFSKSFPILSKYDRLRRSLPILSKYRN 293
             + LPIL  Y      LPIL  Y+   R LPIL  Y    +  PIL  Y  L+R LPIL  Y+ 
Sbjct:  180 QRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQGLQRLLPILLSYQALQRLLPILLSYQA 243

HSP 7 Score: 45.0542 bits (105), Expect = 5.529e-6
Identity = 27/64 (42.19%), Postives = 32/64 (50.00%), Query Frame = -2 
Query:  102 NKSLPILSIYDRFSISLPILSKYRRFSRSLPILSIYDRFSKSFPILSKYDRLRRSLPILSKYRN 293
             + LPIL  Y      LPIL  Y+   R LPIL  Y    +  PIL  Y  L+R LPIL  Y+ 
Sbjct:  193 QRLLPILLSYQALQRLLPILLSYQGLQRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQA 256

HSP 8 Score: 45.0542 bits (105), Expect = 5.529e-6
Identity = 27/64 (42.19%), Postives = 32/64 (50.00%), Query Frame = -2 
Query:  102 NKSLPILSIYDRFSISLPILSKYRRFSRSLPILSIYDRFSKSFPILSKYDRLRRSLPILSKYRN 293
             + LPIL  Y      LPIL  Y+   R LPIL  Y    +  PIL  Y  L+R LPIL  Y+ 
Sbjct:  206 QRLLPILLSYQGLQRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQA 269

HSP 9 Score: 44.669 bits (104), Expect = 6.211e-6
Identity = 27/64 (42.19%), Postives = 32/64 (50.00%), Query Frame = -2 
Query:  102 NKSLPILSIYDRFSISLPILSKYRRFSRSLPILSIYDRFSKSFPILSKYDRLRRSLPILSKYRN 293
             + LPIL  Y      LPIL  Y+   R LPIL  Y    +  PIL  Y  L+R LPIL  Y+ 
Sbjct:  167 QRLLPILLSYQGLQRLLPILLSYQALQRLLPILLSYQALQRLLPILLSYQGLQRLLPILLSYQA 230

HSP 10 Score: 44.669 bits (104), Expect = 7.468e-6
Identity = 27/64 (42.19%), Postives = 32/64 (50.00%), Query Frame = -2 
Query:  102 NKSLPILSIYDRFSISLPILSKYRRFSRSLPILSIYDRFSKSFPILSKYDRLRRSLPILSKYRN 293
             + LPIL  Y      LPIL  Y+   R LPIL  Y    +  PIL  Y  L+R LPIL  Y+ 
Sbjct:  128 QRLLPILLSYKALQRLLPILLSYKALQRLLPILLSYQALQRLLPILLSYQGLQRLLPILLSYQA 191

HSP 11 Score: 44.2838 bits (103), Expect = 8.472e-6
Identity = 27/64 (42.19%), Postives = 32/64 (50.00%), Query Frame = -2 
Query:  102 NKSLPILSIYDRFSISLPILSKYRRFSRSLPILSIYDRFSKSFPILSKYDRLRRSLPILSKYRN 293
             + LPIL  Y      LPIL  Y+   R LPIL  Y    +  PIL  Y  L+R LPIL  Y+ 
Sbjct:  141 QRLLPILLSYKALQRLLPILLSYQALQRLLPILLSYQGLQRLLPILLSYQALQRLLPILLSYQA 204
[Close]
BLAST of Pedal peptide prohormone like-2 vs. TrEMBL
Match: E3CTJ3 (Pedal peptide prohormone like-2 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 4.184e-47
Identity = 96/97 (98.97%), Postives = 97/97 (100.00%), Query Frame = 1 
Query:    4 MTVNGXXXXXXXXXXXXXXVDLSKSREHGEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL 294
            MTVNGILISSMMITIILSTVDLSKSREHGEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDK+GSEMLKRSYMDKIGSDLL
Sbjct:    1 MTVNGILISSMMITIILSTVDLSKSREHGEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKIGSEMLKRSYMDKIGSDLL 97
[Close]
BLAST of Pedal peptide prohormone like-2 vs. TrEMBL
Match: A0A193PE73 (Neuropeptide-35 (Fragment) OS=Dugesia japonica OX=6161 GN=DjNp35 PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.920e-12
Identity = 43/77 (55.84%), Postives = 49/77 (63.64%), Query Frame = 1 
Query:   76 SREHGEIKRFRYFDKIGSDLL-KRSYFDKIGNDLL-KRSYMDKIGSDLLKRR-YFDKMGSEML-KRSYMDKIGSDLL 294
            S   G  KR  Y+D IG  LL KRSY+D IG  LL KRSY D IG  LLK+R Y+D +G  +L KRSY D IG  LL
Sbjct:   18 SMREGPFKRGSYYDPIGGGLLKKRSYYDPIGGGLLKKRSYYDPIGGGLLKKRSYYDPIGGGLLKKRSYYDPIGGSLL 94

HSP 2 Score: 67.0106 bits (162), Expect = 1.248e-11
Identity = 42/73 (57.53%), Postives = 50/73 (68.49%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLK-RSYFDKIGNDLLK-RSYMDKIGSDLLKRR-YFDKMGSEML-KRSYMDKIGSDLL 294
            G +K+  Y+D IG  LLK RSY+D IG  LLK RSY D IG  LLK+R Y+D +G  +L KRSY D IGS LL
Sbjct:   36 GLLKKRSYYDPIGGGLLKKRSYYDPIGGGLLKKRSYYDPIGGGLLKKRSYYDPIGGSLLKKRSYYDPIGSGLL 108

HSP 3 Score: 53.9138 bits (128), Expect = 1.152e-6
Identity = 39/74 (52.70%), Postives = 44/74 (59.46%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLK-RSYFDKIGNDLL-KRSYMDKIGSDLLKRR---YFDKMGSEMLKRSYMDKIGSDLL 294
            G +K+  Y+D IG  LLK RSY+D IG  LL KRSY D IGS LLKRR   Y  K      K +Y D IG  LL
Sbjct:   64 GLLKKRSYYDPIGGGLLKKRSYYDPIGGSLLKKRSYYDPIGSGLLKRRSAAYLQK------KFNYYDPIGGGLL 131
[Close]
BLAST of Pedal peptide prohormone like-2 vs. TrEMBL
Match: V5TCS9 (Pedal peptide neuropeptide 2 MLDpeptide OS=Platynereis dumerilii OX=6359 PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 3.133e-10
Identity = 29/63 (46.03%), Postives = 47/63 (74.60%), Query Frame = 1 
Query:  106 RYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL 294
            R  D++GS LLK+   D++G+ LLK+  +D++GS LLK+R  D++GS +LK+  +D++GS LL
Sbjct:   73 RMLDEVGSSLLKKRMLDEVGSSLLKKRMLDEVGSSLLKKRMLDEVGSSLLKKRMLDEVGSSLL 135

HSP 2 Score: 62.3882 bits (150), Expect = 3.405e-9
Identity = 28/62 (45.16%), Postives = 45/62 (72.58%), Query Frame = 1 
Query:  106 RYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDL 291
            R  D++GS LLK+   D++G+ LLK+  +D++GS LLK+R  D++GS +L++  +D IGS L
Sbjct:   86 RMLDEVGSSLLKKRMLDEVGSSLLKKRMLDEVGSSLLKKRMLDEVGSSLLRKRVVDPIGSAL 147

HSP 3 Score: 58.5362 bits (140), Expect = 8.288e-8
Identity = 28/63 (44.44%), Postives = 45/63 (71.43%), Query Frame = 1 
Query:  106 RYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL 294
            R  D++GS LLK+   D++G+ LLK+  +D++GS LL++R  D +GS  LK+  +D++GS LL
Sbjct:   99 RMLDEVGSSLLKKRMLDEVGSSLLKKRMLDEVGSSLLRKRVVDPIGS-ALKKRMLDEVGSSLL 160

HSP 4 Score: 55.0694 bits (131), Expect = 1.723e-6
Identity = 29/79 (36.71%), Postives = 47/79 (59.49%), Query Frame = 1 
Query:  106 RYFDKIGSDLLKRSY----------------FDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL 294
            R  D++GS LLK+                   D++G+ LLK+  +D++GS LLK+R  D++GS +LK+  +D++GS LL
Sbjct:   44 RMLDQVGSSLLKKRMSPQEDYLMKSQGQKRMLDEVGSSLLKKRMLDEVGSSLLKKRMLDEVGSSLLKKRMLDEVGSSLL 122
[Close]
BLAST of Pedal peptide prohormone like-2 vs. TrEMBL
Match: E3CTL6 (Pedal peptide prohormone like-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 8.624e-9
Identity = 40/73 (54.79%), Postives = 48/73 (65.75%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLKR-SYFDKIGNDLLKR-SYMDKIGSDLLKRR-YFDKMGSEMLK-RSYMDKIGSDLL 294
            G +KR  Y+D IG  LLKR SY+D IG  LLKR S  D IG  LLKRR ++D +G  +LK RS+ D IG  LL
Sbjct:   44 GLLKRGAYYDPIGGGLLKRSSYYDPIGGGLLKRDSNYDPIGGGLLKRRSFYDPIGGGLLKRRSFYDPIGGGLL 116

HSP 2 Score: 58.5362 bits (140), Expect = 3.210e-8
Identity = 38/72 (52.78%), Postives = 47/72 (65.28%), Query Frame = 1 
Query:   91 EIKRFRYFDKIGSDLLKR-SYFDKIGNDLLKR-SYMDKIGSDLLKR-RYFDKMGSEMLK-RSYMDKIGSDLL 294
              K++ Y+D IGS LLKR +Y+D IG  LLKR SY D IG  LLKR   +D +G  +LK RS+ D IG  LL
Sbjct:   31 RFKKYSYYDSIGSGLLKRGAYYDPIGGGLLKRSSYYDPIGGGLLKRDSNYDPIGGGLLKRRSFYDPIGGGLL 102
[Close]
BLAST of Pedal peptide prohormone like-2 vs. TrEMBL
Match: V4BDE1 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_167253 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 4.032e-8
Identity = 26/69 (37.68%), Postives = 43/69 (62.32%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL 294
            G++ +  + D I  DL K+++ D I  DL K+++MD I  DL K+R+ D +  ++ K+S+MD I  DL 
Sbjct:  235 GDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKRHMDSIAGDLFKKSHMDSIAGDLF 303

HSP 2 Score: 59.3066 bits (142), Expect = 1.075e-7
Identity = 27/77 (35.06%), Postives = 47/77 (61.04%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL*KSDLE*I 318
            G+I +  + D I  DL K+++ D I  DL K+++MD I  DL K+ + D +  ++ K+++MD I  DL  K+ ++ I
Sbjct:  131 GDIFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSI 207

HSP 3 Score: 58.9214 bits (141), Expect = 1.410e-7
Identity = 31/84 (36.90%), Postives = 50/84 (59.52%), Query Frame = 1 
Query:   67 LSKSREHGEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL*KSDLE*I 318
            + +S  +G IKR    D   +DL+++   D I  DL KR +MD I SDL K+R+ D +  ++ K+++MD I  DL  K+ ++ I
Sbjct:   74 IKESTANGVIKR--PLDAEAADLIQKRPMDSIAGDLFKRHHMDAIASDLFKKRHVDSLAGDIFKKNHMDSIAGDLFKKNHMDSI 155

HSP 4 Score: 58.151 bits (139), Expect = 2.161e-7
Identity = 27/77 (35.06%), Postives = 46/77 (59.74%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL*KSDLE*I 318
            G++ +  + D I  DL K+++ D I  DL K+++MD I  DL K+ + D +  ++ K+ +MD I  DL  KS ++ I
Sbjct:  222 GDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKRHMDSIAGDLFKKSHMDSI 298

HSP 5 Score: 58.151 bits (139), Expect = 2.782e-7
Identity = 26/77 (33.77%), Postives = 47/77 (61.04%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL*KSDLE*I 318
            G++ +  + D I  DL K+++ D I  DL K+++MD I  DL K+ + D +  ++ K+++MD I  DL  K+ ++ I
Sbjct:  144 GDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSI 220

HSP 6 Score: 58.151 bits (139), Expect = 2.782e-7
Identity = 26/77 (33.77%), Postives = 47/77 (61.04%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL*KSDLE*I 318
            G++ +  + D I  DL K+++ D I  DL K+++MD I  DL K+ + D +  ++ K+++MD I  DL  K+ ++ I
Sbjct:  157 GDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSI 233

HSP 7 Score: 58.151 bits (139), Expect = 2.782e-7
Identity = 26/77 (33.77%), Postives = 47/77 (61.04%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL*KSDLE*I 318
            G++ +  + D I  DL K+++ D I  DL K+++MD I  DL K+ + D +  ++ K+++MD I  DL  K+ ++ I
Sbjct:  170 GDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSI 246

HSP 8 Score: 58.151 bits (139), Expect = 2.782e-7
Identity = 26/77 (33.77%), Postives = 47/77 (61.04%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL*KSDLE*I 318
            G++ +  + D I  DL K+++ D I  DL K+++MD I  DL K+ + D +  ++ K+++MD I  DL  K+ ++ I
Sbjct:  183 GDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSI 259

HSP 9 Score: 58.151 bits (139), Expect = 2.782e-7
Identity = 26/77 (33.77%), Postives = 47/77 (61.04%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL*KSDLE*I 318
            G++ +  + D I  DL K+++ D I  DL K+++MD I  DL K+ + D +  ++ K+++MD I  DL  K+ ++ I
Sbjct:  196 GDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSI 272

HSP 10 Score: 56.9954 bits (136), Expect = 5.488e-7
Identity = 25/77 (32.47%), Postives = 47/77 (61.04%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL*KSDLE*I 318
            G++ +  + D I SDL K+ + D +  D+ K+++MD I  DL K+ + D +  ++ K+++MD I  DL  K+ ++ I
Sbjct:  105 GDLFKRHHMDAIASDLFKKRHVDSLAGDIFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSI 181

HSP 11 Score: 56.9954 bits (136), Expect = 5.760e-7
Identity = 26/77 (33.77%), Postives = 46/77 (59.74%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL*KSDLE*I 318
            G++ +  + D I  DL K+++ D I  DL K+++MD I  DL K+ + D +  ++ K+++MD I  DL  K  ++ I
Sbjct:  209 GDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKNHMDSIAGDLFKKRHMDSI 285
[Close]
BLAST of Pedal peptide prohormone like-2 vs. Planmine SMEST
Match: SMESG000059588.1 (SMESG000059588.1)

HSP 1 Score: 155.992 bits (393), Expect = 1.164e-50
Identity = 96/97 (98.97%), Postives = 97/97 (100.00%), Query Frame = 1 
Query:    4 MTVNGXXXXXXXXXXXXXXVDLSKSREHGEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL 294
            MTVNGILISSMMITIILSTVDLSKSREHGEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDK+GSEMLKRSYMDKIGSDLL
Sbjct:    1 MTVNGILISSMMITIILSTVDLSKSREHGEIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKIGSEMLKRSYMDKIGSDLL 97
[Close]
BLAST of Pedal peptide prohormone like-2 vs. Planmine SMEST
Match: SMESG000059626.1 (SMESG000059626.1)

HSP 1 Score: 72.4034 bits (176), Expect = 8.582e-18
Identity = 37/57 (64.91%), Postives = 46/57 (80.70%), Query Frame = 1 
Query:   91 EIKRFRYFDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKR 261
            +I++ RY DK+G DLLK S   KIG +LLKRSY DKIGSDLLKR Y DK+GS++L+R
Sbjct:   28 DIRQSRYIDKMGDDLLKPSVVYKIGKELLKRSYFDKIGSDLLKRSYIDKIGSDLLRR 84

HSP 2 Score: 71.633 bits (174), Expect = 1.666e-17
Identity = 37/61 (60.66%), Postives = 47/61 (77.05%), Query Frame = 1 
Query:  112 FDKIGSDLLKRSYFDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL 294
            F     D+ +  Y DK+G+DLLK S + KIG +LLKR YFDK+GS++LKRSY+DKIGSDLL
Sbjct:   22 FKSSAVDIRQSRYIDKMGDDLLKPSVVYKIGKELLKRSYFDKIGSDLLKRSYIDKIGSDLL 82

HSP 3 Score: 48.9062 bits (115), Expect = 1.119e-8
Identity = 27/51 (52.94%), Postives = 34/51 (66.67%), Query Frame = 1 
Query:  151 FDKIGNDLLKRSYMDKIGSDLLKRRYFDKMGSEMLKRSYMDKIGSDLL*KS 303
            F     D+ +  Y+DK+G DLLK     K+G E+LKRSY DKIGSDLL +S
Sbjct:   22 FKSSAVDIRQSRYIDKMGDDLLKPSVVYKIGKELLKRSYFDKIGSDLLKRS 72
[Close]
BLAST of Pedal peptide prohormone like-2 vs. Planmine SMEST
Match: SMESG000035027.1 (SMESG000035027.1)

HSP 1 Score: 60.077 bits (144), Expect = 2.398e-12
Identity = 40/73 (54.79%), Postives = 48/73 (65.75%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLKR-SYFDKIGNDLLKR-SYMDKIGSDLLKRR-YFDKMGSEMLK-RSYMDKIGSDLL 294
            G +KR  Y+D IG  LLKR SY+D IG  LLKR S  D IG  LLKRR ++D +G  +LK RS+ D IG  LL
Sbjct:   44 GLLKRGAYYDPIGGGLLKRSSYYDPIGGGLLKRDSNYDPIGGGLLKRRSFYDPIGGGLLKRRSFYDPIGGGLL 116

HSP 2 Score: 58.5362 bits (140), Expect = 8.927e-12
Identity = 38/72 (52.78%), Postives = 47/72 (65.28%), Query Frame = 1 
Query:   91 EIKRFRYFDKIGSDLLKR-SYFDKIGNDLLKR-SYMDKIGSDLLKR-RYFDKMGSEMLK-RSYMDKIGSDLL 294
              K++ Y+D IGS LLKR +Y+D IG  LLKR SY D IG  LLKR   +D +G  +LK RS+ D IG  LL
Sbjct:   31 RFKKYSYYDSIGSGLLKRGAYYDPIGGGLLKRSSYYDPIGGGLLKRDSNYDPIGGGLLKRRSFYDPIGGGLL 102

HSP 3 Score: 49.6766 bits (117), Expect = 2.373e-8
Identity = 30/60 (50.00%), Postives = 41/60 (68.33%), Query Frame = 1 
Query:   88 GEIKRFRYFDKIGSDLLKR-SYFDKIGNDLLKR-SYMDKIGSDLLKRRYF-DKMGSEMLK 258
            G +KR  ++D IG  LLKR S++D IG  LLKR S+ D IG  LLK+R++ D +G  +LK
Sbjct:   86 GLLKRRSFYDPIGGGLLKRRSFYDPIGGGLLKRRSFYDPIGGGLLKKRFYNDPLGVALLK 145
The following BLAST results are available for this feature:
BLAST of Pedal peptide prohormone like-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pedal peptide prohormone like-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pedal peptide prohormone like-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pedal peptide prohormone like-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pedal peptide prohormone like-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSXETT00000023923.13.000e-642.19pep primary_assembly:Xenopus_tropicalis_v9.1:10:33... [more]
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BLAST of Pedal peptide prohormone like-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pedal peptide prohormone like-2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pedal peptide prohormone like-2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
E3CTJ34.184e-4798.97Pedal peptide prohormone like-2 OS=Schmidtea medit... [more]
A0A193PE732.920e-1255.84Neuropeptide-35 (Fragment) OS=Dugesia japonica OX=... [more]
V5TCS93.133e-1046.03Pedal peptide neuropeptide 2 MLDpeptide OS=Platyne... [more]
E3CTL68.624e-954.79Pedal peptide prohormone like-1 OS=Schmidtea medit... [more]
V4BDE14.032e-837.68Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
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BLAST of Pedal peptide prohormone like-2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pedal peptide prohormone like-2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pedal peptide prohormone like-2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pedal peptide prohormone like-2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pedal peptide prohormone like-2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Pedal peptide prohormone like-2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000059588.11.164e-5098.97SMESG000059588.1[more]
SMESG000059626.18.582e-1864.91SMESG000059626.1[more]
SMESG000035027.12.398e-1254.79SMESG000035027.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013280 ID=SMED30013280|Name=Pedal peptide prohormone like-2|organism=Schmidtea mediterranea sexual|type=transcript|length=351bp
ATAATGACAGTCAACGGTATTTTGATTTCGTCAATGATGATCACGATCAT
ACTTTCCACAGTAGACTTGTCAAAGTCCAGAGAACACGGCGAAATCAAGC
GGTTCCGATATTTCGATAAAATTGGAAGTGACCTTCTCAAACGATCGTAT
TTCGATAAAATTGGAAATGATTTACTGAAACGGTCGTATATGGATAAAAT
CGGTAGTGACCTGCTGAAGCGACGATATTTCGATAAAATGGGCAGCGAAA
TGCTGAAGCGGTCGTATATGGACAAAATTGGAAGCGATTTGTTGTAGAAA
TCAGATTTGGAATAAATATAATTGTTTAAATAAATTCGATTATGTTTGTT
A
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protein sequence of SMED30013280-orf-1

>SMED30013280-orf-1 ID=SMED30013280-orf-1|Name=SMED30013280-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=116bp
NKHNRIYLNNYIYSKSDFYNKSLPILSIYDRFSISLPILSKYRRFSRSLP
ILSIYDRFSKSFPILSKYDRLRRSLPILSKYRNRLISPCSLDFDKSTVES
MIVIIIDEIKIPLTVI
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000044cephalic ganglia
PLANA:0000097ventral nerve cord
PLANA:0000418head
Vocabulary: biological process
TermDefinition
GO:0007218neuropeptide signaling pathway
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..26
score: 0.522