SMED30013241

Overview


Name	SMED30013241
Smed ID	SMED30013241
Length (bp)	1050

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30013241 (SMED30013241) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30013241 (SMED30013241) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30013241 (SMED30013241) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30013241 (SMED30013241) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30013241

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30013241	SMESG000000524.1	SmedASXL_032259	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pharynx	SMED30013241	SMESG000007775.1	dd_Smed_v4_137_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
protonephridia	SMED30013241	SMESG000007775.1	dd_Smed_v4_137_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30013241	SMESG000007775.1	dd_Smed_v4_137_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30013241	SMESG000007775.1	dd_Smed_v4_137_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30013241	SMESG000007775.1	dd_Smed_v4_137_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30013241	SMESG000007775.1	dd_Smed_v4_137_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
pharynx	SMED30013241	SMESG000007775.1	dd_Smed_v6_137_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
epidermis	SMED30013241	SMESG000007775.1	dd_Smed_v6_137_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
Category 3 cell	SMED30013241	SMESG000007775.1	dd_Smed_v6_137_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
epidermis	SMED30013241	SMESG000007775.1	dd_Smed_v4_137_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
Category 4 cell	SMED30013241	SMESG000007775.1	dd_Smed_v4_137_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
dorsal region of the epidermis	SMED30013241	SMESG000007775.1	dd_Smed_v4_137_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of SMED30013241 vs. Ensembl Human
Match: DYNLL2 (dynein light chain LC8-type 2 [Source:HGNC Symbol;Acc:HGNC:24596])

HSP 1 Score: 52.373 bits (124), Expect = 2.645e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Human
Match: DYNLL1 (dynein light chain LC8-type 1 [Source:HGNC Symbol;Acc:HGNC:15476])

HSP 1 Score: 51.9878 bits (123), Expect = 3.249e-8
Identity = 29/69 (42.03%), Postives = 41/69 (59.42%), Query Frame = -2

Query:  426 VEDVFQNLES-NAER--AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 623
            VE   Q LE  N E+  A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   22 VECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

Query:  426 VEDVFQNLES-NAER--AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 623
            VE   Q LE  N E+  A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   22 VECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

Query:  426 VEDVFQNLES-NAER--AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 623
            VE   Q LE  N E+  A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   22 VECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

Query:  426 VEDVFQNLES-NAER--AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 623
            VE   Q LE  N E+  A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   22 VECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Celegans
Match: dlc-1 (Dynein light chain 1, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q22799])

HSP 1 Score: 53.9138 bits (128), Expect = 3.024e-9
Identity = 25/84 (29.76%), Postives = 44/84 (52.38%), Query Frame = -2

Query:  426 TIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 677
             I+++  +DD  +   +      +      + A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:    7 VIKNADMSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

Query:  426 TIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 677
             I+++  +DD  +   +      +      + A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:    7 VIKNADMSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

Query:  426 TIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 677
             I+++  +DD  +   +      +      + A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:    7 VIKNADMSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Fly
Match: ctp (gene:FBgn0011760 transcript:FBtr0070706)

HSP 1 Score: 52.373 bits (124), Expect = 1.127e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Fly
Match: ctp (gene:FBgn0011760 transcript:FBtr0070704)

HSP 1 Score: 52.373 bits (124), Expect = 1.127e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Fly
Match: ctp (gene:FBgn0011760 transcript:FBtr0307065)

HSP 1 Score: 52.373 bits (124), Expect = 1.127e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Fly
Match: ctp (gene:FBgn0011760 transcript:FBtr0070707)

HSP 1 Score: 52.373 bits (124), Expect = 1.127e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Fly
Match: Cdlc2 (gene:FBgn0026141 transcript:FBtr0077924)

HSP 1 Score: 51.9878 bits (123), Expect = 1.856e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Zebrafish
Match: dynll2a (dynein, light chain, LC8-type 2a [Source:ZFIN;Acc:ZDB-GENE-030828-11])

HSP 1 Score: 52.373 bits (124), Expect = 1.838e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Zebrafish
Match: dynll2b (dynein, light chain, LC8-type 2b [Source:ZFIN;Acc:ZDB-GENE-050320-132])

HSP 1 Score: 52.373 bits (124), Expect = 1.838e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Zebrafish
Match: dynll1 (dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:ZDB-GENE-040426-1961])

HSP 1 Score: 51.9878 bits (123), Expect = 2.514e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Xenopus
Match: dynll2 (dynein light chain LC8-type 2 [Source:NCBI gene;Acc:100038056])

HSP 1 Score: 52.373 bits (124), Expect = 2.398e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Xenopus
Match: dynll1 (dynein light chain LC8-type 1 [Source:NCBI gene;Acc:448652])

HSP 1 Score: 51.6026 bits (122), Expect = 4.189e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Mouse
Match: Dynll2 (dynein light chain LC8-type 2 [Source:MGI Symbol;Acc:MGI:1915347])

HSP 1 Score: 52.373 bits (124), Expect = 1.816e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Mouse
Match: Dynll1 (dynein light chain LC8-type 1 [Source:MGI Symbol;Acc:MGI:1861457])

HSP 1 Score: 51.9878 bits (123), Expect = 2.230e-8
Identity = 29/69 (42.03%), Postives = 41/69 (59.42%), Query Frame = -2

Query:  426 VEDVFQNLES-NAER--AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 623
            VE   Q LE  N E+  A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   22 VECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

Query:  426 VEDVFQNLES-NAER--AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 623
            VE   Q LE  N E+  A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   22 VECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. UniProt/SwissProt
Match: sp|Q6BZF8|DYL1_DEBHA (Dynein light chain 1, cytoplasmic OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=DYN2 PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 5.260e-9
Identity = 28/86 (32.56%), Postives = 48/86 (55.81%), Query Frame = -2

Query:  426 PVTIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 683
            P+ +++S   ++   RI E+  +   N +   + A  +K +LDQ +   W  IVGK+F +YVTHE+  F +  +G Q    +FK+G
Sbjct:    8 PI-LKASDLPEEMQTRIFELSNEAVSNYKIEKDIATYLKKELDQLYGATWHVIVGKSFGSYVTHEQGFFTYFYIG-QLAFLIFKSG 91

[Close]

BLAST of SMED30013241 vs. UniProt/SwissProt
Match: sp|Q22799|DYL1_CAEEL (Dynein light chain 1, cytoplasmic OS=Caenorhabditis elegans OX=6239 GN=dlc-1 PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 3.847e-8
Identity = 25/84 (29.76%), Postives = 44/84 (52.38%), Query Frame = -2

Query:  426 TIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 677
             I+++  +DD  +   +      +      + A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:    7 VIKNADMSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. UniProt/SwissProt
Match: sp|O02414|DYL1_HELCR (Dynein light chain LC6, flagellar outer arm OS=Heliocidaris crassispina OX=1043166 PE=3 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 1.065e-7
Identity = 26/88 (29.55%), Postives = 44/88 (50.00%), Query Frame = -2

Query:  426 EMPVTIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 689
            E    I+++   +D  +   +      +      + A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  V +FK+G
Sbjct:    3 ERKAVIKNADMPEDMQQDAVDCATQALEKFNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAVLLFKSG 89

[Close]

BLAST of SMED30013241 vs. UniProt/SwissProt
Match: sp|Q24117|DYL1_DROME (Dynein light chain 1, cytoplasmic OS=Drosophila melanogaster OX=7227 GN=ctp PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 1.129e-7
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. UniProt/SwissProt
Match: sp|Q78P75|DYL2_RAT (Dynein light chain 2, cytoplasmic OS=Rattus norvegicus OX=10116 GN=Dynll2 PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 1.270e-7
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. TrEMBL
Match: A0A2G8LHP4 (Dynein light chain OS=Stichopus japonicus OX=307972 GN=BSL78_03349 PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.142e-8
Identity = 30/85 (35.29%), Postives = 48/85 (56.47%), Query Frame = -2

Query:  426 VTIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 680
            V +++S   DD  +  ++I ++ F   +   + A  IK + D +F   W CIVG+NF +YVTHE   F ++ V  Q  V +FK+G
Sbjct:   44 VVVKNSDMEDDMKEEATKITQEAFDQFKVEKDIAAYIKKEFDSKFGAAWHCIVGRNFGSYVTHETRNFIYMYV-KQVAVLLFKSG 127

[Close]

BLAST of SMED30013241 vs. TrEMBL
Match: G8BSQ3 (Dynein light chain OS=Tetrapisispora phaffii (strain ATCC 24235 / CBS 4417 / NBRC 1672 / NRRL Y-8282 / UCD 70-5) OX=1071381 GN=TPHA0D02370 PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.048e-8
Identity = 33/86 (38.37%), Postives = 48/86 (55.81%), Query Frame = -2

Query:  432 EMPVTIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFK 689
            E P+ +++S   DD    I +I  + F   +   + A  IK +LD +F   W  IVGKNF +YVTHE+  F +  +GS T + VFK
Sbjct:    7 ESPI-LKASDINDDMKDEIFKISTEAFNEFKVERDIAGSIKKQLDTKFGNTWHVIVGKNFGSYVTHEKGHFIYFYIGS-TALLVFK 90

[Close]

BLAST of SMED30013241 vs. TrEMBL
Match: A0A5K3G490 (Dynein light chain OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.737e-8
Identity = 29/85 (34.12%), Postives = 48/85 (56.47%), Query Frame = -2

Query:  432 MPVTIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFK 686
            M V + SS   + + K  ++++ +         + AK IK+  D+++   W CIVGK+FA YVTHE++ F H  +G+   V +FK
Sbjct:   14 MKVNVRSSDMPEAQQKEAAKVIAEALSIHTKETQVAKTIKSHFDEKYGRAWHCIVGKHFAGYVTHEQNTFMHAHIGN-VSVMLFK 97

[Close]

BLAST of SMED30013241 vs. TrEMBL
Match: A0A0F6MV57 (Dynein light chain OS=Spirometra erinaceieuropaei OX=99802 PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 3.568e-8
Identity = 28/84 (33.33%), Postives = 46/84 (54.76%), Query Frame = -2

Query:  426 TIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 677
             ++++  +D+  +R  EI ++         + A  IK   D+EF   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:    7 VVKNADMSDEMQQRAVEIGQEALDKFNIEKDIAAHIKKSFDKEFNPTWHCIVGRNFGSYVTHESKHFIYSYMG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. TrEMBL
Match: A0A0R3TD46 (Dynein light chain OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS4983 PE=3 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 4.088e-8
Identity = 29/84 (34.52%), Postives = 45/84 (53.57%), Query Frame = -2

Query:  426 TIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 677
             I+++  TDD  ++  +  +D         + A  IK + D+ F   W CIVGKNF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   12 VIKNADMTDDMQQKAVDCAQDAMNKYSVEKDVAAHIKKEFDRIFGPTWHCIVGKNFGSYVTHETRHFIYFYMG-QVAILLFKSG 94

[Close]

BLAST of SMED30013241 vs. Ensembl Cavefish
Match: ENSAMXT00000047143.1 (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:103041528])

HSP 1 Score: 52.373 bits (124), Expect = 1.557e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Cavefish
Match: dynll2b (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:103041528])

HSP 1 Score: 52.373 bits (124), Expect = 1.557e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Cavefish
Match: dynll1 (dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:ZDB-GENE-040426-1961])

HSP 1 Score: 49.6766 bits (117), Expect = 2.386e-7
Identity = 27/68 (39.71%), Postives = 39/68 (57.35%), Query Frame = -2

Query:  435 EIVEDVFQNLES-NAER--AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVF 629
            + VE   Q LE  N E+  A  IK + D+++   W CIVG+NF +YVTHE   F +  +G + G  V+
Sbjct:   20 DAVECATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQRNGWAVW 87

[Close]

BLAST of SMED30013241 vs. Ensembl Yeast
Match: DYN2 (Cytoplasmic light chain dynein, microtubule motor protein; required for intracellular transport and cell division; involved in mitotic spindle positioning; forms complex with dynein intermediate chain Pac11p that promotes Dyn1p homodimerization, potentiates motor processivity; Dyn2p-Pac11p complex important for interaction of dynein motor complex with dynactin complex; acts as molecular glue to dimerize, stabilize Nup82-Nsp1-Nup159 complex module of cytoplasmic pore filaments [Source:SGD;Acc:S000002832])

HSP 1 Score: 49.6766 bits (117), Expect = 1.724e-8
Identity = 26/79 (32.91%), Postives = 42/79 (53.16%), Query Frame = -2

Query:  459 NFEMPVTIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVG 695
            N   P+ +++S  TD   + I  I +D     +   + A  +K +LD ++   W  IVGKNF +YVTHE+  F +  +G
Sbjct:    5 NKSTPI-VKASDITDKLKEDILTISKDALDKYQLERDIAGTVKKQLDVKYGNTWHVIVGKNFGSYVTHEKGHFVYFYIG 82

[Close]

BLAST of SMED30013241 vs. Ensembl Nematostella
Match: EDO44582 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVI5])

HSP 1 Score: 52.373 bits (124), Expect = 5.415e-9
Identity = 26/88 (29.55%), Postives = 43/88 (48.86%), Query Frame = -2

Query:  426 EMPVTIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 689
            E    I+++   +D      E      +      + A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:    3 ERKAVIKNADMAEDMQTDAIECATQALEKFNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Medaka
Match: dynll1 (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:101171417])

HSP 1 Score: 52.7582 bits (125), Expect = 9.487e-9
Identity = 25/84 (29.76%), Postives = 44/84 (52.38%), Query Frame = -2

Query:  426 TIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 677
             I+++  ++D  +   E      +      + A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:    7 VIKNADMSEDMQQDAVECATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Medaka
Match: ENSORLT00000005747.2 (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:101162462])

HSP 1 Score: 51.9878 bits (123), Expect = 1.938e-8
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  IK + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Ensembl Medaka
Match: DYNLL2 (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:101174042])

HSP 1 Score: 51.2174 bits (121), Expect = 3.353e-8
Identity = 21/52 (40.38%), Postives = 33/52 (63.46%), Query Frame = -2

Query:  426 AKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 581
            A  +K + D+++   W CIVG+NF +YVTHE   F +  +G Q  + +FK+G
Sbjct:   39 AAYVKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLG-QVAILLFKSG 89

[Close]

BLAST of SMED30013241 vs. Planmine SMEST
Match: SMESG000007775.1 (SMESG000007775.1)

HSP 1 Score: 184.111 bits (466), Expect = 1.647e-58
Identity = 88/88 (100.00%), Postives = 88/88 (100.00%), Query Frame = -2

Query:  423 MPVTIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAGY 686
            MPVTIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAGY
Sbjct:    1 MPVTIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAGY 88

[Close]

BLAST of SMED30013241 vs. Planmine SMEST
Match: SMESG000008800.1 (SMESG000008800.1)

HSP 1 Score: 80.8777 bits (198), Expect = 6.172e-19
Identity = 36/84 (42.86%), Postives = 56/84 (66.67%), Query Frame = -2

Query:  429 VTIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKA 680
            +T+  SK + ++N+ I E V ++F    +N ERAK+IK KLD +F   WQ I+G +F T +THE+ + C +KVG + G+Q+FK 
Sbjct:    8 ITLIDSKMSTEDNEFIVETVGNIFNEKNTNRERAKDIKTKLDIQFGTSWQVIIGLSFTTMITHEQDQICTLKVGEKIGIQMFKT 91

[Close]

BLAST of SMED30013241 vs. Planmine SMEST
Match: SMESG000009941.1 (SMESG000009941.1)

HSP 1 Score: 78.1814 bits (191), Expect = 6.448e-18
Identity = 36/85 (42.35%), Postives = 54/85 (63.53%), Query Frame = -2

Query:  426 VTIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 680
            + ++ +K   +    I EIV+ VF+  ES  +RA+ IK +LD +FE  W  ++GK F T+VT+EE K+CH+  G  T VQ+FK G
Sbjct:    6 IEVKETKMDSELTHEIKEIVKQVFEECESAKDRAERIKKELDDKFEQYWHVVIGKQFTTFVTYEESKYCHLVCGGNTAVQIFKCG 90

[Close]

BLAST of SMED30013241 vs. Planmine SMEST
Match: SMESG000008800.1 (SMESG000008800.1)

HSP 1 Score: 75.0998 bits (183), Expect = 5.465e-17
Identity = 33/74 (44.59%), Postives = 50/74 (67.57%), Query Frame = -2

Query:  429 DENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKA 650
            ++N+ I E V ++F    +N ERAK+IK KLD +F   WQ I+G +F T +THE+ + C +KVG + G+Q+FK 
Sbjct:    4 EDNEFIVETVGNIFNEKNTNRERAKDIKTKLDIQFGTSWQVIIGLSFTTMITHEQDQICTLKVGEKIGIQMFKT 77

[Close]

BLAST of SMED30013241 vs. Planmine SMEST
Match: SMESG000008798.1 (SMESG000008798.1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.209e-12
Identity = 28/87 (32.18%), Postives = 52/87 (59.77%), Query Frame = -2

Query:  426 MPVTIESSKCTDDENKRISEIVEDVFQNLESNAERAKEIKAKLDQEFEGPWQCIVGKNFATYVTHEEHKFCHVKVGSQTGVQVFKAG 686
            M   +  ++ + ++  ++ EI E  F+  + + +R   IK K+D++F   W  I+GK+F T + +EE  FCH ++ +  GVQV+K+G
Sbjct:    1 MLTKVIETEMSTEKTDQMVEIFEKAFEENDLDCDRVVAIKKKVDEQFGTNWHVIIGKHFITKINYEEDNFCHCRMPNNMGVQVYKSG 87

The following BLAST results are available for this feature:

BLAST of SMED30013241 vs. Ensembl Human
BLAST of SMED30013241 vs. Ensembl Celegans
BLAST of SMED30013241 vs. Ensembl Fly
BLAST of SMED30013241 vs. Ensembl Zebrafish
BLAST of SMED30013241 vs. Ensembl Xenopus
BLAST of SMED30013241 vs. Ensembl Mouse
BLAST of SMED30013241 vs. UniProt/SwissProt
BLAST of SMED30013241 vs. TrEMBL
BLAST of SMED30013241 vs. Ensembl Cavefish
BLAST of SMED30013241 vs. Ensembl Sea Lamprey
BLAST of SMED30013241 vs. Ensembl Yeast
BLAST of SMED30013241 vs. Ensembl Nematostella
BLAST of SMED30013241 vs. Ensembl Medaka
BLAST of SMED30013241 vs. Planmine SMEST

BLAST of SMED30013241 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
DYNLL2	2.645e-8	42.31	dynein light chain LC8-type 2 [Source:HGNC Symbol;...	[more]
DYNLL1	3.249e-8	42.03	dynein light chain LC8-type 1 [Source:HGNC Symbol;...	[more]
DYNLL1	3.249e-8	42.03	dynein light chain LC8-type 1 [Source:HGNC Symbol;...	[more]
DYNLL1	3.249e-8	42.03	dynein light chain LC8-type 1 [Source:HGNC Symbol;...	[more]
DYNLL1	3.249e-8	42.03	dynein light chain LC8-type 1 [Source:HGNC Symbol;...	[more]

BLAST of SMED30013241 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3


Match Name	E-value	Identity	Description
dlc-1	3.024e-9	29.76	Dynein light chain 1, cytoplasmic [Source:UniProt...	[more]
dlc-1	3.024e-9	29.76	Dynein light chain 1, cytoplasmic [Source:UniProt...	[more]
dlc-1	3.024e-9	29.76	Dynein light chain 1, cytoplasmic [Source:UniProt...	[more]

BLAST of SMED30013241 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ctp	1.127e-8	42.31	gene:FBgn0011760 transcript:FBtr0070706	[more]
ctp	1.127e-8	42.31	gene:FBgn0011760 transcript:FBtr0070704	[more]
ctp	1.127e-8	42.31	gene:FBgn0011760 transcript:FBtr0307065	[more]
ctp	1.127e-8	42.31	gene:FBgn0011760 transcript:FBtr0070707	[more]
Cdlc2	1.856e-8	42.31	gene:FBgn0026141 transcript:FBtr0077924	[more]

BLAST of SMED30013241 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
dynll2a	1.838e-8	42.31	dynein, light chain, LC8-type 2a [Source:ZFIN;Acc:...	[more]
dynll2b	1.838e-8	42.31	dynein, light chain, LC8-type 2b [Source:ZFIN;Acc:...	[more]
dynll2a	1.838e-8	42.31	dynein, light chain, LC8-type 2a [Source:ZFIN;Acc:...	[more]
dynll1	2.514e-8	42.31	dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:Z...	[more]
dynll1	2.514e-8	42.31	dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:Z...	[more]

BLAST of SMED30013241 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2


Match Name	E-value	Identity	Description
dynll2	2.398e-8	42.31	dynein light chain LC8-type 2 [Source:NCBI gene;Ac...	[more]
dynll1	4.189e-8	42.31	dynein light chain LC8-type 1 [Source:NCBI gene;Ac...	[more]

BLAST of SMED30013241 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Dynll2	1.816e-8	42.31	dynein light chain LC8-type 2 [Source:MGI Symbol;A...	[more]
Dynll2	1.816e-8	42.31	dynein light chain LC8-type 2 [Source:MGI Symbol;A...	[more]
Dynll2	1.816e-8	42.31	dynein light chain LC8-type 2 [Source:MGI Symbol;A...	[more]
Dynll1	2.230e-8	42.03	dynein light chain LC8-type 1 [Source:MGI Symbol;A...	[more]
Dynll1	2.230e-8	42.03	dynein light chain LC8-type 1 [Source:MGI Symbol;A...	[more]

BLAST of SMED30013241 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q6BZF8\|DYL1_DEBHA	5.260e-9	32.56	Dynein light chain 1, cytoplasmic OS=Debaryomyces ...	[more]
sp\|Q22799\|DYL1_CAEEL	3.847e-8	29.76	Dynein light chain 1, cytoplasmic OS=Caenorhabditi...	[more]
sp\|O02414\|DYL1_HELCR	1.065e-7	29.55	Dynein light chain LC6, flagellar outer arm OS=Hel...	[more]
sp\|Q24117\|DYL1_DROME	1.129e-7	42.31	Dynein light chain 1, cytoplasmic OS=Drosophila me...	[more]
sp\|Q78P75\|DYL2_RAT	1.270e-7	42.31	Dynein light chain 2, cytoplasmic OS=Rattus norveg...	[more]

BLAST of SMED30013241 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A2G8LHP4	1.142e-8	35.29	Dynein light chain OS=Stichopus japonicus OX=30797...	[more]
G8BSQ3	2.048e-8	38.37	Dynein light chain OS=Tetrapisispora phaffii (stra...	[more]
A0A5K3G490	2.737e-8	34.12	Dynein light chain OS=Mesocestoides corti OX=53468...	[more]
A0A0F6MV57	3.568e-8	33.33	Dynein light chain OS=Spirometra erinaceieuropaei ...	[more]
A0A0R3TD46	4.088e-8	34.52	Dynein light chain OS=Rodentolepis nana OX=102285 ...	[more]

BLAST of SMED30013241 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
ENSAMXT00000047143.1	1.557e-8	42.31	dynein light chain 2, cytoplasmic [Source:NCBI gen...	[more]
dynll2b	1.557e-8	42.31	dynein light chain 2, cytoplasmic [Source:NCBI gen...	[more]
dynll1	2.386e-7	39.71	dynein, light chain, LC8-type 1 [Source:ZFIN;Acc:Z...	[more]

BLAST of SMED30013241 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30013241 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
DYN2	1.724e-8	32.91	Cytoplasmic light chain dynein, microtubule motor ...	[more]

BLAST of SMED30013241 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO44582	5.415e-9	29.55	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30013241 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
dynll1	9.487e-9	29.76	dynein light chain 2, cytoplasmic [Source:NCBI gen...	[more]
ENSORLT00000005747.2	1.938e-8	42.31	dynein light chain 2, cytoplasmic [Source:NCBI gen...	[more]
DYNLL2	3.353e-8	40.38	dynein light chain 2, cytoplasmic [Source:NCBI gen...	[more]

BLAST of SMED30013241 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000007775.1	1.647e-58	100.00	SMESG000007775.1	[more]
SMESG000008800.1	6.172e-19	42.86	SMESG000008800.1	[more]
SMESG000009941.1	6.448e-18	42.35	SMESG000009941.1	[more]
SMESG000008800.1	5.465e-17	44.59	SMESG000008800.1	[more]
SMESG000008798.1	2.209e-12	32.18	SMESG000008798.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30013241 ID=SMED30013241|Name=SMED30013241|organism=Schmidtea mediterranea sexual|type=transcript|length=1050bp
TTATTGTTGATGATATACTAGTTTTCACTTTAAATTCATAAAGCACATGA
TAAATTAATTCATTTTCGATAAAATCAATGAAAAACCCAAAAATATACCT
CTTAAACGAATAAAGTGGGAGATTTTACTAATTGAAGTTGAGCAATTAAA
AGTTACATTATTTTTTGTAATTATCTACGACATATGTTTTTAAACTGTTG
CGGGCGTAGAAAAAACAAAATTAAATTGAAGGTCCGCATATTTTAGATTT
TATACGAGTGTTCCGTGAAGTAAAACGTTTAAGAACCACTGGACAAAAAT
ATAATATTACCGCAAAATAAAATACATATTAAAATGTTTNTTTTTTTGAA
AGATAAAATTAAAATTTATTAAAATTTTTCTAACAAGTCATAACATAATT
TGAAAATAAACTATTTTCTTTAATATCCAGCTTTGAAAACTTGGACTCCA
GTTTGAGAACCGACTTTCACATGACAGAATTTATGTTCCTCATGAGTAAC
GTATGTTGCGAAATTCTTTCCAACAATGCATTGCCATGGGCCTTCAAATT
CCTGGTCTAGTTTGGCTTTAATTTCTTTTGCTCGTTCAGCATTTGACTCC
AAATTTTGAAAAACATCTTCGACAATTTCGGAAATTCGTTTATTTTCATC
ATCGGTACATTTGCTACTTTCAATTGTTACTGGCATTTCAAAATTTTATT
TTGCTGNNAATGAAGAAATNNNNNNTANNTNNNNANANNNNNNNNNGTTT
CTGGCTGGCCAGTTATTTGTGTATATATCCGATATTTTTATACAAGCGGC
TATTAATGTAGACCGACTACTGTAAATATGATGCTGCAGTGTTCCTTTTT
ATTAGGCTGACACCGACAGCAAGGGCAACATTGAATGCTTTAGGCGATAT
TTTCACTTTTTGTACGTATGAGACGATTATGAGCCGCAGCGGCCGCCGCC
ATTGCCGCCGCCGCCGCCGCCGCCTCCGCCGCCTCACCTCAGTTTCTGTA
TGTCTAGCACAAATTTTCCTTGGATTATGAATAAATCTTTTTGATTAAAA

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000018	Category 4 cell
PLANA:0000020	protonephridia
PLANA:0000034	epidermis
PLANA:0000035	Category 3 cell
PLANA:0000054	dorsal region of the epidermis
PLANA:0000418	head
PLANA:0002032	epidermal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR037177	DLC_sf
IPR001372	Dynein_light_chain_typ-1/2

Vocabulary: **cellular component**
Term	Definition
GO:0030286	dynein complex

Vocabulary: **biological process**
Term	Definition
GO:0007017	microtubule-based process

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 26..46
None	No IPR available	PANTHER	PTHR11886:SF35	DYNEIN LIGHT CHAIN 2, CYTOPLASMIC	coord: 4..87
IPR001372	Dynein light chain, type 1/2	SMART	SM01375	Dynein_light_2	coord: 1..86 e-value: 2.5E-19 score: 80.2
IPR001372	Dynein light chain, type 1/2	PFAM	PF01221	Dynein_light	coord: 4..85 e-value: 6.9E-20 score: 71.2
IPR001372	Dynein light chain, type 1/2	PANTHER	PTHR11886	DYNEIN LIGHT CHAIN	coord: 4..87
IPR037177	Dynein light chain superfamily	GENE3D	G3DSA:3.30.740.10		coord: 2..87 e-value: 6.9E-20 score: 72.9
IPR037177	Dynein light chain superfamily	SUPERFAMILY	SSF54648	DLC	coord: 3..86

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SMED30013241

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Owlery

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