SMED30013161

Overview
NameSMED30013161
Smed IDSMED30013161
Length (bp)2175
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30013161 (SMED30013161) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30013161 (SMED30013161) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30013161 (SMED30013161) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30013161 (SMED30013161) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30013161

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 25

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30013161SMESG000023005.1 Contig639GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30013161SMESG000051379.1 Contig639GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30013161SMESG000051379.1 dd_Smed_v4_2630_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30013161SMESG000051379.1 dd_Smed_v4_2630_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30013161SMESG000051379.1 dd_Smed_v4_2630_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30013161SMESG000051379.1 dd_Smed_v4_2630_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30013161SMESG000051379.1 dd_Smed_v4_2630_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30013161SMESG000051379.1 dd_Smed_v4_2630_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30013161SMESG000051379.1 dd_Smed_v4_2630_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30013161SMESG000051379.1 dd_Smed_v4_2630_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013161SMESG000051379.1 Contig41398newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013161SMESG000051379.1 Contig41398uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013161SMESG000051379.1 Contig31286newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013161SMESG000051379.1 Contig31286uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013161SMESG000051379.1 Contig44170newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013161SMESG000051379.1 Contig44170uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30013161SMESG000051379.1 dd_Smed_v6_2630_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30013161SMESG000051379.1 dd_Smed_v6_2630_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30013161SMESG000051379.1 dd_Smed_v6_2630_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30013161SMESG000051379.1 dd_Smed_v6_2630_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30013161SMESG000051379.1 dd_Smed_v6_2630_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gamma neoblastSMED30013161SMESG000051379.1 dd_Smed_v6_2630_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30013161SMESG000051379.1 Contig31286newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30013161SMESG000051379.1 Contig31286uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30013161SMESG000051379.1 dd_Smed_v4_2630_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30013161 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002292:28606..61229 -8506
Homology
BLAST of SMED30013161 vs. TrEMBL
Match: A0A3P9HL64 (Uncharacterized protein OS=Oryzias latipes OX=8090 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 3.510e-7
Identity = 78/278 (28.06%), Postives = 136/278 (48.92%), Query Frame = 2
Query:  524 LNVIKKLELYFKSINNVSVDESTIENSSMNDQLIEYKKQIKEKEAIINEKNDEIEQLKEK------QIDK-DSKIENFKTQIKSLSEEIQHKIDKIEAINKNIKNLKDQLVGKDEKIDQLTQEIKNLQRHAPSL-TQPEEVKKSNPEMKKTLEEKIQLITELEAKLNKLESKNWEKGENQEKIKELENKIRQLMIDNENIYEDVRXXXXXXXXXXXXXXXXRDIKISELDNENMEFEGKIRKLEREIKGLRRKNLENEHEVADLESQKEELETKIKNL 1333
            L  I++LE   K +    + E   +   +  Q+ E + QI E E  I E   +I QL+ +      QI + + +I+  + QI+ L  +I+  + +I  +   I+ L+ Q+   + ++ +L  +I+ L      L  Q +E++    E++  + E +  I ELE ++ +LE +  E  E Q  IKELE +IR+L      +   +R                 + +I EL+ +  EFEG+IR+LE +I+ L  +  E E ++  LE Q  ELE +IK L
Sbjct:  121 LGQIRELEGQIKELE-AQIGELEGQIKELEGQIGELEGQIGELEGQIRELEGQIRQLEGQIRELLGQIGQLEGQIKELEGQIRELEGQIKELVGQIRELEGQIRELEGQIRELEGQLRELVGQIRELVGQIRELEGQIKELEGQIKELEGQIRELLGQIRELEGQIKELEGQIREL-EGQ--IKELEGQIRELEGQIRELEGQIREL---------------EAQIGELEGQIKEFEGQIRELEGQIRELEGQIRELEGQIRKLEGQIRELEGQIKEL 379          
BLAST of SMED30013161 vs. Ensembl Medaka
Match: ENSORLT00000035643.1 (pep primary_assembly:ASM223467v1:3:935033:1013328:1 gene:ENSORLG00000028201.1 transcript:ENSORLT00000035643.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.6842 bits (130), Expect = 3.015e-7
Identity = 75/291 (25.77%), Postives = 141/291 (48.45%), Query Frame = 2
Query:  533 IKKLELYFKS-INNVSVDESTIENSSMNDQLIEYKKQIKEKEAIINEKNDEIEQLKEKQIDK--------DSKIENFKTQIKSLSEEIQHKIDKIEAINKNIKNLKDQLVGKDEKIDQLTQEIKNLQRHAPSL-TQPEEVKKSNPEMKKTLEEKIQLITELEAKLNKLES--KNWEKGENQEKIKELENKIRQLMIDNENIYEDVRXXXXXXXXXXXXXXXXRDIK-----ISELDNENMEFEGKIRKLEREIKGLRRKNLENEHEVADLESQKEELETKIKNLKKENRTL 1354
            I++LE   +  +  +   E  I    +  Q+ + + QI++ E  I +   +I QL E QI +        + +I   + QI+ L  +I+  + +I  +   I+ L+ Q+   + +I QL  +I+ L+     L  Q  E++    +++  + E    I +LE ++ +LE   ++ E      +I+ELE +IR+L      +   +R+   +    +  +  E  I+     I EL+ +  E EG+IR LE +I+GL  +  E E ++ +LE Q  ELE +I  L+ + R L
Sbjct:  221 IRELEGQIRQLVGQIRQLEGQI--RQLEGQIRQLEGQIRQLEGQIRQLVGQIRQL-EGQIRQLVGQIRQLEGQIRQLEGQIRQLEGQIRQLVGQIRGLEGQIRQLEGQIRQLEGQIRQLEGQIRQLEGKIRQLEGQIRELEGQIRQLEGQIRELEGQIRQLEGQIRQLEGQIRHLEG-----QIRELEGQIRELEGQIRELEGQIRHLEGQIRELEGHQGAEGQIRGLEGQIRELEGQIRELEGQIRGLEGQIRGLEGQIRELEGQIRELEGQIRELEGQIGELEGQIREL 503          

HSP 2 Score: 53.1434 bits (126), Expect = 8.861e-7
Identity = 67/256 (26.17%), Postives = 128/256 (50.00%), Query Frame = 2
Query:  605 SMNDQLIEYKKQIKEKEAIINEKNDEIEQLKEKQIDK-DSKIENFKTQIKSLSEEIQHKIDKIEAINKNIKNLKDQ---LVGKDEKIDQLTQEIKNLQRHAPSLTQPEEVKKSNPEMKKTLEEKIQLITELEAKLNKL--ESKNWEKGENQEKIKELENKIRQLMIDNENIYEDVRXXXXXXXXXXXXXXXXRDIKISELDNENMEFEGKIRKLEREIKGLRRKNLENEHEVADLESQKEELETKIKNLKKENRTL 1354
             +  Q+ + + QI++ E  I +   +I QL E QI + + +I   + QI+ L  +I+  + +I  +   I+ L+ Q   LVG+   I QL  +I+ L+     L    ++++   ++++ LE +I    ELE ++ +L  + +  E      +I+ELE +IRQL+     +   +R                 + +I +L+ +  + EG+IR+LE +I+ L  +  + E ++  LE Q  ELE +I+ L  + R L
Sbjct:   13 QLEGQIRQLEGQIRQLEGQIRQLEGQIRQL-EGQIRQLEGQIRQLEGQIRELEGQIRQLVGQIRQLEGQIRELEGQIRQLVGQ---IRQLEGQIRQLEGQIRQL--EGQIRQLEGQIRQ-LEGQI---RELEGQIRQLVGQIRQLEG-----QIRELEGQIRQLVGQIRQLEGQIRQL---------------EGQIRQLEGQIRQLEGQIRELEGQIRQLEGQIRQLEGQIRQLEGQIRELEGQIRQLVGQIRQL 238          

HSP 3 Score: 50.447 bits (119), Expect = 5.702e-6
Identity = 67/279 (24.01%), Postives = 134/279 (48.03%), Query Frame = 2
Query:  533 IKKLELYFKSI-NNVSVDESTIENSSMNDQLIEYKKQIKEKEAIINEKNDEIEQLKEKQIDK-DSKIENFKTQIKSLSEEIQHKIDKIEAINKNIKNLKDQLVGKDEKIDQLTQEIKNLQRHAPSLTQPEEVKKSNPEMKKTLEEKIQL---ITELEAKLNKLESKNWEKGENQEKIKELENKIRQLMIDNENIYEDVRXXXXXXXXXXXXXXXXRDIKISELDNENMEFEGKIRKLEREIKGLRRKNLENEHEVADLESQKEELETKIKNLKKENRTL 1354
            I++LE   + +   +   E  I    +  Q+ + + QI+E E  I +   +I QL E QI + + +I     QI+ L  +I+    +I  +   I+ L+ Q+   + +I QL  +I+ L+     L    ++++   ++++ + +  QL   I +LE ++ +LE +          I++LE +IRQL+     +   +R    +            + +I +L+ +  + EG+IR+L  +I+GL       E ++  LE Q  +LE +I+ L+ + R L
Sbjct:   95 IRQLEGQIRQLEGQIRQLEGQI--RQLEGQIRQLEGQIRELEGQIRQLVGQIRQL-EGQIRELEGQIRQLVGQIRQLEGQIRQLEGQIRQLEGQIRQLEGQIRELEGQIRQLEGQIRQLEGQIRQL--EGQIRELEGQIRQLVGQIRQLEGQIRQLEGQIRQLEGQ----------IRQLEGQIRQLVGQIRQLEGQIRQLVGQIRQL--------EGQIRQLEGQIRQLEGQIRQLVGQIRGL-------EGQIRQLEGQIRQLEGQIRQLEGQIRQL 343          
BLAST of SMED30013161 vs. Planmine SMEST
Match: SMESG000051379.1 (SMESG000051379.1)

HSP 1 Score: 1008.05 bits (2605), Expect = 0.000e+0
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 2
Query:  149 MSNDFSLVGYESVSIAEYLPILENIKHDLKFINEKFNVKHTLGTMDFDTVTVNSTLTTPVNNEETAVCDVAMNTDLTLRDIAMNTSSVDTNDSRSLHPECEQQFMEKLQRIELLIPRNHDNNDNSLNVIKKLELYFKSINNVSVDESTIENSSMNDQLIEYKKQIKEKEAIINEKNDEIEQLKEKQIDKDSKIENFKTQIKSLSEEIQHKIDKIEAINKNIKNLKDQLVGKDEKIDQLTQEIKNLQRHAPSLTQPEEVKKSNPEMKKTLEEKIQLITELEAKLNKLESKNWEKGENQEKIKELENKIRQLMIDNENIYEDVRXXXXXXXXXXXXXXXXRDIKISELDNENMEFEGKIRKLEREIKGLRRKNLENEHEVADLESQKEELETKIKNLKKENRTLKXXXXXXXXXXXXXXXXXXLKTALKVKENELNKTITXXXXXXXXXXXXXXXXXXXXXXXXXXXMLEKEKQNTEIEKLKTEIENLKRECEKKQQAILTMRQKLSQKNEDKSSPNEVKKLQNQMKAKDMEIDQVHAKVENLNKAFEEKCVEHDMSVQQIENYKTEIENLKRLQFTAKPNILPKPSG 1906
            MSNDFSLVGYESVSIAEYLPILENIKHDLKFINEKFNVKHTLGTMDFDTVTVNSTLTTPVNNEETAVCDVAMNTDLTLRDIAMNTSSVDTNDSRSLHPECEQQFMEKLQRIELLIPRNHDNNDNSLNVIKKLELYFKSINNVSVDESTIENSSMNDQLIEYKKQIKEKEAIINEKNDEIEQLKEKQIDKDSKIENFKTQIKSLSEEIQHKIDKIEAINKNIKNLKDQLVGKDEKIDQLTQEIKNLQRHAPSLTQPEEVKKSNPEMKKTLEEKIQLITELEAKLNKLESKNWEKGENQEKIKELENKIRQLMIDNENIYEDVRNEFEKSLNSKKKELKERDIKISELDNENMEFEGKIRKLEREIKGLRRKNLENEHEVADLESQKEELETKIKNLKKENRTLKNTTQNEEEQTNNSEEVNSLKTALKVKENELNKTITELKKKLAESQEKLKEKEKELIGELEAKMLEKEKQNTEIEKLKTEIENLKRECEKKQQAILTMRQKLSQKNEDKSSPNEVKKLQNQMKAKDMEIDQVHAKVENLNKAFEEKCVEHDMSVQQIENYKTEIENLKRLQFTAKPNILPKPSG
Sbjct:    1 MSNDFSLVGYESVSIAEYLPILENIKHDLKFINEKFNVKHTLGTMDFDTVTVNSTLTTPVNNEETAVCDVAMNTDLTLRDIAMNTSSVDTNDSRSLHPECEQQFMEKLQRIELLIPRNHDNNDNSLNVIKKLELYFKSINNVSVDESTIENSSMNDQLIEYKKQIKEKEAIINEKNDEIEQLKEKQIDKDSKIENFKTQIKSLSEEIQHKIDKIEAINKNIKNLKDQLVGKDEKIDQLTQEIKNLQRHAPSLTQPEEVKKSNPEMKKTLEEKIQLITELEAKLNKLESKNWEKGENQEKIKELENKIRQLMIDNENIYEDVRNEFEKSLNSKKKELKERDIKISELDNENMEFEGKIRKLEREIKGLRRKNLENEHEVADLESQKEELETKIKNLKKENRTLKNTTQNEEEQTNNSEEVNSLKTALKVKENELNKTITELKKKLAESQEKLKEKEKELIGELEAKMLEKEKQNTEIEKLKTEIENLKRECEKKQQAILTMRQKLSQKNEDKSSPNEVKKLQNQMKAKDMEIDQVHAKVENLNKAFEEKCVEHDMSVQQIENYKTEIENLKRLQFTAKPNILPKPSG 586          
The following BLAST results are available for this feature:
BLAST of SMED30013161 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013161 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013161 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013161 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013161 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013161 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013161 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013161 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A3P9HL643.510e-728.06Uncharacterized protein OS=Oryzias latipes OX=8090... [more]
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BLAST of SMED30013161 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013161 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013161 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013161 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013161 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000035643.13.015e-725.77pep primary_assembly:ASM223467v1:3:935033:1013328:... [more]
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BLAST of SMED30013161 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000051379.10.000e+0100.00SMESG000051379.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013161 ID=SMED30013161|Name=SMED30013161|organism=Schmidtea mediterranea sexual|type=transcript|length=2175bp
ATTACCATTTATTTAAATCATAGATCCTCTTATCATATTAATATTACATC
CAATTACATCAATAACAAAAACTTAAAAAACCCACTTTGATTTGGTTAAA
TTAAAGTTCAGAATTGAAATAAATTGTCATTATAGATAAATATTCAAAAT
GTCTAACGACTTTTCTTTGGTAGGATACGAAAGTGTTTCAATTGCAGAAT
ATTTGCCAATTTTAGAAAATATAAAACACGATTTAAAATTCATCAACGAG
AAATTTAACGTTAAACACACATTAGGAACTATGGATTTTGATACTGTAAC
AGTGAATAGCACTTTAACAACTCCAGTGAATAATGAAGAGACGGCTGTAT
GTGATGTTGCTATGAATACAGACCTGACTTTACGTGATATCGCTATGAAT
ACATCCTCGGTCGACACCAATGATAGCCGTTCTCTACATCCCGAATGTGA
GCAGCAATTTATGGAAAAGTTACAAAGAATAGAACTTCTAATTCCTAGGA
ATCATGATAATAATGACAATAGCCTCAATGTAATTAAAAAATTGGAACTT
TATTTCAAATCGATTAATAATGTAAGTGTTGATGAGAGCACAATTGAAAA
TTCCAGCATGAATGATCAACTAATTGAATACAAAAAACAAATCAAAGAAA
AAGAGGCCATTATCAATGAGAAAAATGATGAAATTGAACAATTAAAAGAG
AAACAAATAGACAAAGATTCAAAAATTGAAAATTTTAAAACACAAATAAA
AAGTCTAAGTGAGGAAATACAGCACAAGATTGATAAAATTGAAGCTATTA
ATAAGAATATTAAAAACCTTAAAGATCAATTAGTAGGCAAAGATGAAAAG
ATAGATCAATTAACACAAGAAATTAAGAATTTGCAACGTCATGCTCCATC
ACTAACACAACCTGAAGAAGTTAAGAAATCCAATCCAGAAATGAAGAAAA
CCTTAGAGGAAAAAATTCAACTTATTACTGAATTGGAAGCAAAGCTGAAT
AAATTAGAATCTAAAAACTGGGAAAAGGGCGAAAATCAAGAAAAAATTAA
AGAGCTAGAAAATAAGATTCGACAATTGATGATTGACAATGAAAATATTT
ATGAAGATGTACGAAATGAATTTGAGAAAAGTTTGAATAGTAAGAAAAAA
GAACTCAAAGAAAGAGATATAAAAATCTCTGAACTTGATAATGAGAATAT
GGAATTTGAAGGTAAAATTAGAAAATTGGAACGAGAGATTAAAGGATTAA
GGAGAAAAAATTTAGAAAATGAGCATGAAGTTGCAGATTTAGAAAGTCAG
AAAGAAGAATTAGAAACGAAAATCAAAAATCTGAAAAAAGAAAATAGGAC
GTTGAAAAATACAACTCAAAATGAAGAGGAACAGACAAACAATTCAGAAG
AAGTTAACAGTTTGAAAACAGCCTTGAAAGTTAAAGAAAATGAATTAAAT
AAAACTATCACTGAATTAAAGAAAAAATTAGCGGAATCCCAAGAAAAACT
AAAAGAAAAAGAAAAAGAATTAATAGGTGAACTAGAAGCCAAAATGTTGG
AAAAAGAAAAGCAAAATACTGAAATTGAAAAACTGAAAACCGAAATCGAA
AATCTTAAAAGAGAATGTGAAAAAAAACAACAAGCAATTCTTACCATGCG
TCAGAAACTCAGTCAGAAAAATGAAGACAAATCATCTCCAAATGAGGTAA
AGAAATTACAAAACCAAATGAAAGCAAAGGATATGGAAATTGATCAAGTA
CATGCAAAAGTAGAAAATCTGAATAAAGCATTTGAGGAAAAATGTGTTGA
GCATGACATGTCTGTTCAACAAATTGAAAACTACAAGACAGAAATTGAAA
ATTTAAAGAGACTTCAGTTTACTGCTAAACCAAATATTTTGCCAAAGCCA
AGTGGAGTATGGTTTTGGATGTTAATATCGAAAATTATATTTCTGAATGT
AGAATACAGAAAATGAGTTTCCTCAATGGTGGCGAAAACATATGGATAGA
TAATCATCACTCTAATTGTGGAAAATACATTATTTTACATTCAGTACAAA
TATATTTGTGTAGTAACCTGCTTTGAAATTTGTTGAATGGTTTTGTACTT
GACACAAGTTTTGTTTCTCTAATGACAGCTATTAATAATTAACTTTAATT
TATACATTTATAGAATTTGTATATG
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protein sequence of SMED30013161-orf-1

>SMED30013161-orf-1 ID=SMED30013161-orf-1|Name=SMED30013161-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=606bp
MSNDFSLVGYESVSIAEYLPILENIKHDLKFINEKFNVKHTLGTMDFDTV
TVNSTLTTPVNNEETAVCDVAMNTDLTLRDIAMNTSSVDTNDSRSLHPEC
EQQFMEKLQRIELLIPRNHDNNDNSLNVIKKLELYFKSINNVSVDESTIE
NSSMNDQLIEYKKQIKEKEAIINEKNDEIEQLKEKQIDKDSKIENFKTQI
KSLSEEIQHKIDKIEAINKNIKNLKDQLVGKDEKIDQLTQEIKNLQRHAP
SLTQPEEVKKSNPEMKKTLEEKIQLITELEAKLNKLESKNWEKGENQEKI
KELENKIRQLMIDNENIYEDVRNEFEKSLNSKKKELKERDIKISELDNEN
MEFEGKIRKLEREIKGLRRKNLENEHEVADLESQKEELETKIKNLKKENR
TLKNTTQNEEEQTNNSEEVNSLKTALKVKENELNKTITELKKKLAESQEK
LKEKEKELIGELEAKMLEKEKQNTEIEKLKTEIENLKRECEKKQQAILTM
RQKLSQKNEDKSSPNEVKKLQNQMKAKDMEIDQVHAKVENLNKAFEEKCV
EHDMSVQQIENYKTEIENLKRLQFTAKPNILPKPSGVWFWMLISKIIFLN
VEYRK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000039gamma neoblast
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0002032epidermal cell
PLANA:0002089reproductive organ
PLANA:0003116parenchymal cell
Vocabulary: molecular function
TermDefinition
GO:0003700transcription factor activity, sequence-specific DNA binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
Vocabulary: biological process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 517..544
NoneNo IPR availableCOILSCoilCoilcoord: 262..320
NoneNo IPR availableCOILSCoilCoilcoord: 552..572
NoneNo IPR availableCOILSCoilCoilcoord: 419..503
NoneNo IPR availableCOILSCoilCoilcoord: 151..248
NoneNo IPR availableCOILSCoilCoilcoord: 329..415
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 502..526
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 399..421
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 504..526
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 406..421
NoneNo IPR availablePANTHERPTHR34491FAMILY NOT NAMEDcoord: 145..570