Limbin

Overview
NameLimbin
Smed IDSMED30013159
Length (bp)3547
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Limbin (SMED30013159) t-SNE clustered cells

Violin plots show distribution of expression levels for Limbin (SMED30013159) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Limbin (SMED30013159) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Limbin (SMED30013159) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013159

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 15

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30013159SMESG000017303.1 SmedASXL_015639SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30013159SMESG000017303.1 dd_Smed_v4_10777_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30013159SMESG000017303.1 dd_Smed_v4_10777_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30013159SMESG000017303.1 dd_Smed_v4_10777_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30013159SMESG000017303.1 dd_Smed_v4_10777_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30013159SMESG000017303.1 dd_Smed_v4_10777_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30013159SMESG000017303.1 dd_Smed_v4_10777_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
cilated neuronSMED30013159SMESG000017303.1 dd_Smed_v4_10777_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013159SMESG000017303.1 Contig53400newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013159SMESG000017303.1 Contig53400uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013159SMESG000031560.1 SMESG000022722.1 SMESG000017303.1 Contig41423newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013159SMESG000031560.1 SMESG000022722.1 SMESG000017303.1 Contig41423uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30013159SMESG000017303.1 dd_Smed_v6_10777_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30013159SMESG000017303.1 dd_Smed_v4_10777_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30013159SMESG000017303.1 dd_Smed_v4_10777_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Limbin vs. TrEMBL
Match: A0A210Q549 (Limbin OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT17598 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 3.960e-25
Identity = 159/664 (23.95%), Postives = 328/664 (49.40%), Query Frame = 3 
Query: 1329 KEILKNSENNKNLEGISAESASELIKNYLDWQQQLQSIKEKEKIRQLGRLDYIFQKRNNFIKLKENEINDTNEVMEKKNEFFVEMINNLKDREELDGRVC-ETILLDYEKYKKDLF---KEMDTSRRDQNDKMLSLLNQKRNEKLNDLKNQHDNEIVNLLASHSA--DNNIKSLS--DDLKNIESKYYEKVLNEENSHDAEGVNEIIKIHNEISTQTKEKLDSEIILLTKSLKSEGVDSGDIRSILDSYDKRFKSLKNTQNKERERQLMEMNEKIRKFKENIDKNVEKQINEKDQLFQSENNIVEELINIQKTLSEDDRDYILKEYNKHKISTQNSIANSFLEQKRKMEEALALRKAKQLKEIDDKHDLKVEELINRKMRVKTKRTQQEIKEESEKELMLDYVNQKLQLSSVGNSKDDLDKELEIIRNELLAERAALVKKKEEEYVALILKVQMLKAKESAELKDQEEKI--FKLQLAALTSSKA----QNSQEIFKVYQNTKENLKIELDKNKEIQESTLKEKLENR--KRNTLRKKTE------------NEPANDDSETIRRKQQSIEDQKLKNEIN---------------MER--SIQNVEKDMLKKCISSSDCSREEVFNLMLEIYPLKSSQEIEELVEE-FCPKSRQPPQNNQLKSG 3182
            K +LK+ E + NL     E    L+  + + Q++L    + E  +Q  +L+   ++R    KL E   +D ++ +     +    +N+   R + DG V  +   + +EK KK++    ++MD  RR Q + +   L+  + +++ +L+ Q + E+ + + SHSA  D  +  LS  +    +ESK+ E+     +  D E  +++  +  EISTQT+E L +    L  +LK++G      R ++  +      L+ +Q      Q++ + E++ + + +++    ++  E+  L + E  +V  LI  Q  +S+D+RD IL+E+ K  +  +N++  + L QKR +EE L  ++ +Q+ ++ +K  L+  +   RK     + +  E ++E    L L +  + + L      K +LD E+E +R E+L +RA  +K +EE    ++  +Q+ KA E ++++DQ++ I   K+ L    + +     Q  QEI + Y+  ++ L+  L   +   E  LK++L+ R  +R  +  +T+            N+ A    + +   +Q IE ++++N ++               MER   IQ  E  ++ + IS     +E++ N++  ++P K+ ++I++++ + + P+  QP   ++ K G
Sbjct:  514 KTMLKDLEVDGNLHD---EKVQWLLDEHKESQKRLDLSYDNEISKQRMQLEEKLERRRGLSKLSEQNEDDMSDTLN----YLASNMNDFITRAKKDGIVSPDEAKVMFEKAKKEMIALKEQMDKDRRKQEEDLHKKLSNLKKQRMANLQKQQEGELQSYMKSHSAQTDGPVDPLSLVEGKLQLESKHREEAHKVSDQMDQEHAHDLATLREEISTQTEEDLSNIQTQLINNLKNQGFSEDTARVLIKKHRDAMAKLQESQEANLHNQMVNIQERLERNRADLEMRKAQEKQEQALLREREEKLVNNLITGQMVMSDDERDRILQEHEKQMVKLENNLTLNKLRQKRMLEEKLMEKRDRQMHKLQEKQ-LQQTQAQKRKQENNAEESDDESRKEKTNLLKLQFEQRMVILH---GEKLNLDDEMENVRVEMLKQRALALKNQEERLGGMVAALQVSKAHELSKIEDQQKAINDLKMNLMDDLNERGVLGNQECQEIIERYKEEQDKLQHSLADRQRQHEKALKKRLKQRLQQREKVMAETQEKELQEILSQSKNKVAAKIKKALLLHKQMIEMEQMRNRLDREISQTLRETQRLFEMERLQKIQEQELLVISRLISIGGFEKEDLNNVLRTLFPSKTEEDIKKIMSKIYDPQKMQPAPVSRDKDG 1166
[Close]
BLAST of Limbin vs. TrEMBL
Match: A0A1S3HPJ5 (trichohyalin-like OS=Lingula unguis OX=7574 GN=LOC106157035 PE=4 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 1.659e-23
Identity = 186/795 (23.40%), Postives = 397/795 (49.94%), Query Frame = 3 
Query: 1005 IAEEINQASLKEYNEKAK----------KIAMES----ISL-NKMNLEYLSNNQEEER---ERIMNLTSSTYKEKIESDLAKVFEHQHHDLISQLKIKENLDMEKLKLTQEELLNSQLNDLYLSAVKEILKNSENNKNLEGISAESASELIKNYLDWQQQLQSIKEKEKIRQLGRLDYIFQKRNNFIKLKENEINDTNEVMEKKNEFFVEMINNLKDREELDGRVCETILLDYEKYKKDLFKEM-------DTSRRDQNDKMLSLLNQKRNEKLND---LKNQHDNEIVNLLASHSADNNIKSLSDDLKN--IESKYYEKVLNEENSHDAEGVNEIIKIHNEISTQTKEKLDSEIILLTKSLKSEGVDSGD-IRSILDSYDKRFKSLKNTQNKERERQLMEMNEKIRKFKENIDKNVEKQINEKDQLFQSENNIVEELINIQKTLSEDDRDYILKEYNKHKISTQNSIANSFLEQKRKMEEALALRKAKQLKEIDDKHDLKVEELINRKMRVKTKRTQQEIKEESEKELMLDYVNQKLQLSSVGNSKDDLDKELEIIRNELLAERAALVKKKEEEYVALILKVQMLKAKESAELKDQEEKIFKLQLAALTSSKAQN------SQEIFKVYQNTKENLKIELDKNKEIQESTLKEKL---------------ENRKRNTLRKKTENEPANDDSETIRRKQQS-IEDQK-------------LKNEINME--RSIQNVEKDMLKKCISSSDCSREEVFNLMLEIYPLKSSQEIEELVEEFCPKSRQPPQNNQLKSGI 3185
            I+ +  +A+LKE+  K K          K+AME     IS+ NK  +  +   Q+EE+   ER MN  S   K ++   L K  + Q  D +  LK++++ + EKL+        + + D+    + +I++  E N+       + A  +I  +   Q+++  I + E  RQ   L+    +R     + + E ++ +EV+    E  ++++  L+ +E +D         D E+Y++++ +EM       DT R  Q  ++   L + + +KL +    + Q  +E+        A +N+  ++   K   + SK+  ++   EN  D E   E++ +  +++ +TKE+L++        LK+ G    D I+ IL  ++ +   L+  Q  +RE+Q   +  K+++ K+ + +  E +  E++Q+ + E N+ +++++ Q  +S+++RD I+KE+ K  +  +NS+  + L QKR +E+ L  ++AK ++ ++ K  LK  + + +K+  KT   ++E + ++   LM  +  QK  L+ +   K  L++E+E IR +++ ERAA +K +E++  A++ ++Q+ KA+E +++  Q++ IF L+ + +     Q       +Q + + +Q  +E L  +L++ KE Q+  L+EKL               E++ ++ L+  +    A   + +++ KQ + +E  K             +K E NM+  +++Q  E + +   +     + +EV +++  ++P+K ++E  +L++          +N + K+G+
Sbjct:  466 ISRQKEEAALKEFMRKVKEMEASMENQYKVAMEEVFRMISIKNKEQISIILKKQKEEKDKLERQMNTASDEEKAEVFEILRKEHDIQQQDAVHLLKLEQDEESEKLRKEFSIKKRAAVKDIQRQCLSQIVEQGELNQ-------QQADWIINEHQKNQEKVDKIYDDEVSRQRAALEEKLTRRRMLASMADGEEDEYSEVLNTLAEQELQVVQKLEKQEIMDED-------DAERYREEIAREMLAAKNRYDTERARQEAELKKRLREAKKQKLAEKAKFQQQELDELQKKQKEEQAQDNVDPIAVIEKQAIMTSKHRAEMTELENQIDLEQTRELLTLREKVTDETKEQLETNRQAFLDKLKNNGFMEDDQIKQILKQHNLQLNKLELAQQTDREKQQENLQRKLKEKKKELQQRREAEKLEQEQIREYEKNLTKKMLDNQVAVSDEERDRIMKEHEKQMVKLENSLTLNKLHQKRMLEKKLTEKRAKHMETLEKKQ-LKETKRVEKKL--KTADEEEEEQHQAAIALMRKHAEQK--LAVLQGDKLKLEEEMEQIREDMIKERAAALKAQEDQLGAMVAEMQLKKAEELSKIDQQQKAIFNLKSSLMEELNTQGVMSDPDTQRVLEKHQEQQEQLNKKLEQQKERQQRLLQEKLSEKMMQREKTMMMEHEDQLKSLLQTTSNKTAARFKAVSLKIKQAAELEAMKNDAVTEMNQKIESMKREFNMKHMQAVQQKELEFISGLVKVGQFNEKEVADMINWLFPMKDTEEKNKLLQMVYQGDDATKKNERPKTGL 1241
[Close]
BLAST of Limbin vs. TrEMBL
Match: A0A2C9M4A7 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106079940 PE=4 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 3.778e-23
Identity = 165/644 (25.62%), Postives = 317/644 (49.22%), Query Frame = 3 
Query: 1323 AVKEILKNSENNKNLEG-ISAESASELIKNYLDWQQQLQSIKEKEKIRQLGRLDYIFQKRNNFIKLKENEINDTNEVMEKKNEFFVEMINNLKDREELDGRVCETILLDYEKYKKDLFKEMDTSRRDQNDKMLSLLNQKRNEKLNDLKNQHDNEIVNLLASHSA---DNNIKSLS--DDLKNIESKYYEKVLNEENSHDAEGVNEIIKIHNEISTQTKEKLDSEIILLTKSLKSEGVDSGDIRSILDSYDKRFKSLKNTQNKERERQLMEMNEKIRKFKENIDKNVEKQINEKDQLFQSENNIVEELINIQKTLSEDDRDYILKEYNKHKISTQNSIANSFLEQKRKMEEALALRKAKQLKEIDDKHDLKVEELINRKMRVKTKRTQQEIKEESEKELMLDYVNQKLQLSSVGNSKDDLDKELEIIRNELLAERAALVKKKEEEYVALILKVQMLKAKESAELKDQEEKIFKLQ---LAALTSSKAQNSQEIFKV---YQNTKENLKIELDKNKEIQESTLKEKLENR---KRNTLRKKTENE-------PANDDSETIRRK---------------------QQSIEDQKLKNEINMERSIQNVEKDMLKKCISSSDCSREEVFNLMLEIYPLKSSQEIEELV 3125
             +KEI +   N+   +G +++  A  L+K +   Q ++    + E  RQ   L+    KR       E + +D  E++ +     V ++  +K  + L     E+ +   +   K++  EMD        ++   L Q + + L +  +Q   ++ + +A + A   D  +  +S    L N++S++ +++ + EN  D E   E+     ++S   KEKL S    L + LK++GV    I  IL  ++K  K ++  Q  +R+ Q  +M  ++ K K+   +   ++  E++ L + E  IV++L++ Q  +SE+DRD ILKE+ K  +  +NS+  + L QKR +EE +ALR+A+Q++E+  K + +    I +  R+     ++E +E   KEL L   + + Q++ +     + +KEL+ IR E++ ERA ++K +EE+  A I  +QM KA+E A++++Q++ I  L+   L  L S    ++ E  ++   ++  +E L  +LDK +  QE  L+ KL+ R   +   L KK E E        +N  +  I+R                       Q++ED+K    +  ++ IQ  E   L   ++     + E+ N++  ++PL++ + I E++
Sbjct:  452 GIKEIQQQFINDVIKDGDMTSTKAEWLLKQHRQQQDEIAHKFDDEISRQRMILEEKLAKRRTLAHASEVQEDDHKEILNEIASCQVNLVQTIKLAKSLTDEQMESQIEQLKGDLKNVKDEMDKKSELNQQELFKKLTQLKRKALTEKMSQQKLQLQDYMAKNKATETDPPVDPISYVTGLVNLKSQHRQELSDLENQLDEEHARELAIEREKLSELAKEKLKSIEKHLIEKLKAQGVLDDTIERILRKHEKTVKEIEAKQITDRKNQETKMKAELAKRKKEFAEMRAREKQEQNDLRRYETEIVDKLLDSQFAISEEDRDRILKEHEKQLVKLENSLTLNKLRQKRILEEKIALRRAQQMEELQQKQEAE----IVKHRRIAESNGEEEDEETLLKELKLKKKHAEQQMALIQGLDFNFEKELDEIRTEMVKERALMLKSQEEKMGAFIATLQMNKAREMAKIEEQQKAINNLKANLLDDLNSRGILSTPECERIILRHKEEQEELNEKLDKERLKQEKVLRSKLQERLQQREELLVKKQEEEIKMLLEQSSNKYAAKIKRALIIHKNLVEMEKFKNKLQREINQTLEDRKRDFNLQQQKMIQEEELKFLAGLVTIGSFEQHELLNVLRMLFPLRTEEAIIEML 1091
[Close]
BLAST of Limbin vs. TrEMBL
Match: A0A433U9J5 (Uncharacterized protein (Fragment) OS=Elysia chlorotica OX=188477 GN=EGW08_001740 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 2.029e-22
Identity = 136/528 (25.76%), Postives = 263/528 (49.81%), Query Frame = 3 
Query: 1725 LNQKRNEKLNDLKNQHDNEIVNLLASHSADN-----NIKSLSDDLKNIESKYYEKVLNEENSHDAEGVNEIIKIHNEISTQTKEKLDSEIILLTKSLKSEGVDSGDIRSILDSYDKRFKSLKNTQNKERERQLMEMNEKI-RKFKENIDKNVEKQINEKDQLFQSENNIVEELINIQKTLSEDDRDYILKEYNKHKISTQNSIANSFLEQKRKMEEALALRKAKQLKEIDDKHDLKVEELINRKMRVKTKRTQQEIKEESEKELMLDYVNQKLQLSSVGNSKDDLDKELEIIRNELLAERAALVKKKEEEYVALILKVQMLKAKESAELKDQEEKIFKLQLAALTSSKAQN------SQEIFKVYQNTKENLKIELDKNKEIQESTLKEKLENR---KRNTLRKKTENE-------PANDDSETIRR-----KQ----------------QSIEDQKLKNEINMERSIQNVEKDMLKKCISSSDCSREEVFNLMLEIYPLKSSQEIEELV----EEFCPKSRQPPQNN 3167
            L+Q +++ L +  NQ  +E+   L S  ++      +  S  D L +++S++ +++   EN  D     E+  + ++  ++ KE+L++    L + +KSEGV    I  IL  + K    L   Q   R+ Q  ++ +++ R+ +E  D+ + ++  ++D L Q E  +V++L+  Q   SE +RD ILKE+ K  +  +NS+  + L QK+ MEE +A R+A+Q++++  K +   E + +RK          E  ++ E +++  +  Q+  ++++     +++ E E IR E+  ERA L+K +EE   ALI  +QM KA+E A +++Q++ I  L+   +    A+        +++  V++  +E L  +LD  +  QE  L+ KL+ R   + N++ ++ E+E         N  S  IRR     KQ                Q++ED K   E+  +   Q  E   L   +     SR E+ +++  ++P K+ + I +++    +E  P + +  QNN
Sbjct:  651 LSQAKHKALAEKMNQQASELNKYLQSSKSEQSEGPVDPVSYVDGLVSLKSRHRKELCVLENELDESHARELESLRSQAVSKAKEELEALERTLIEKMKSEGVSDSVIDKILTDHKKTMNILSARQLANRQNQEAKLKDELARRKREWEDRRLTERQEQQD-LRQHEAEVVDKLLGSQVAFSEQERDRILKEHEKQMVMFENSLTLNKLRQKQMMEEKIAQRRARQMEDLQQKQN--TELMKHRKYAENNGEEDDEETQDKEIQILRKHAEQR--IAAIQAIDFNVEAEAEEIRVEMTKERALLLKDQEERLGALIASLQMNKAREMARIEEQQKAINSLKANLMDDLNARGILSTPECEKVLNVHKEEQERLSRKLDDQRAKQEQMLQSKLQERLAQRENSMLQRQESEMRMLMESTNNKTSANIRRVILIHKQMVQMEKFRNKMEREISQTLEDTKRDFEMQKQHIFQEQEMKFLAGLVRIGHLSRNELIDVLHMLFPGKTEESILDILSKIYQEESPLNWKDSQNN 1173
[Close]
BLAST of Limbin vs. TrEMBL
Match: K1PHU8 (Limbin OS=Crassostrea gigas OX=29159 GN=CGI_10004546 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 9.013e-22
Identity = 156/642 (24.30%), Postives = 324/642 (50.47%), Query Frame = 3 
Query: 1374 ISAESASELIKNYLDWQQQLQSIKEKEKIRQLGRLDYIFQKRNNFIKLKENEINDTNEVMEKKNEFFVEMINNLKDREELDGRVCETILLDYEKYKKDLF---KEMDTSRRDQNDKMLSLLNQKRNEKLNDLKNQHDNEIVNLLASHSADNNIKSLSDDLKNIESKYYEKVLNEENSHDAEGVNEIIKIHNEISTQTKEKLD-SEIILLTKSLKSEGVDSGDIRSILDSYDKRFKSLKNTQNKERERQLMEMNEKIRKFKENIDKNVEKQINEKDQLFQSENNIVEELINIQKTLSEDDRDYILKEYNKHKISTQNSIANSFLEQKRKMEEALALRKAKQLKEIDDKHDLKVEELINRKMRVKTKRTQQEIKEESEK---ELMLDYVNQKLQLSSVGNSKDDLDKELEIIRNELLAERAALVKKKEEEYVALILKVQMLKAKESAELKDQEEKI--FKLQLAALTSSKA----QNSQEIFKVYQNTKENLKIELDKNKEIQE----STLKEKLEN----------RKRNTLRKKTENEPANDDSETI-----------------RRKQQSIEDQKLKNEINMERSIQNVEKDMLKKCISSSDCSREEVFNLMLEIYPLKSSQEIEELVEE-FCPKSRQPPQN 3164
            +  E A  L++ +   Q Q++ + ++E  RQ   L+   +KR    KL+E + +D ++V+       +E+IN  K ++ + G   +       K K+D+    + +D  R+ Q   +   L   + +KLN +K+Q  +E+          + +  +   LK +E+K+ E+V  EEN  D +   ++  +++E++   K++L  +E  L+ +  +   V       +L  ++     L+  Q +E E+Q   +N K+ K + + ++  E++  E++++ + E+ +V++LI+ Q ++SE++RD I+KE+ K  +  +NS+  + L QKR +EE LA ++A Q+ +++ K  ++ E    +KMR + +   +E   E+++   ELM    +QK+ +  +   K ++D ELE ++ E+L ER   +K++EE+  A++  +Q+ KA+E A +++Q+  I   K  L    S +        Q++   ++  +ENL  +L+  K  QE      LKEK+E           R+   L   ++N+ A    + +                 R+  Q++ED +L+ E+   +  Q  E   +   +      ++E+ +++  +YP K+  EI+ +++E + P  +Q P++
Sbjct:  551 LVGEKADWLLEEHRKVQAQMEKMYDEEVARQRILLEEKLEKRKALAKLEEQQEDDGSDVLNTMASHQIELINKAKAKK-VGGLTPDEASELIAKAKEDMISLKERLDKDRQKQEMALAKRLGDMKRKKLNQMKSQQKSELDKTFLEFI--HPLLFVETKLK-LEAKHREEVNQEENEIDEDHAEQLRHLNDELTDHAKDELSRAERNLVKRIGEINTVSPDQYEKLLSEHEADVMRLQAQQKRELEKQKANLNAKLAKSRRDWEQRKEQEKAEQEEIRKREDEVVKKLISGQMSMSEEERDRIMKEHEKQMVKVENSLTLNKLRQKRMLEERLAAKRALQMDKLEKKQSVEYE----KKMR-QIEHNGEESDPENQREKLELMKLQFSQKMAV--LQGQKLNIDDELENVKIEMLKERVIALKEQEEKLGAMVAALQVAKAREMALIEEQQAAINNLKSNLIDELSERGILSDPECQKVLDRHKQEQENLNKKLESQKSKQEKELRKRLKEKIEQKEEIMSRAHKREIEALLATSKNKTALKMKQALLAHKHMMEMEKFRNNLDRQIAQTLEDVRLQFEMRRAKETQQEELRFIAGLVRVGSFDQQELIDVLHILYPQKTDTEIQAVMKEIYDPNFQQAPKD 1181
[Close]
BLAST of Limbin vs. Ensembl Cavefish
Match: lama5 (laminin, alpha 5 [Source:ZFIN;Acc:ZDB-GENE-030131-9823])

HSP 1 Score: 55.4546 bits (132), Expect = 4.829e-7
Identity = 108/498 (21.69%), Postives = 197/498 (39.56%), Query Frame = 3 
Query:  897 FSEFNKTLFNIWLNNVNEKYPSRKSKSDSIRDGVYKIAEEINQASLKEYNEKAKKIAMESISLNKMNLEYLSNNQEEERERIMNL--TSSTYKEKIESDLAKVFEHQHHDLISQLKIKENLDMEKLKLTQEELLNSQLNDLYLSAVKEILKNSENNKNLEGISAESASELIKNYLD-WQQQLQSIK--------------EKEKIRQLGRLDYIFQKRNNFIKLKENEINDTNEVMEKKNEFFVEMINNLKDREELDGRVCETILLDYEKYKKDLFKEMDTSRRDQNDKMLSLLNQKRNEKLNDLKNQHDNEIVNLLASHSADNNIKSLSDDLKNIESKYYEKVLNEENSHD--AEGVNEIIKIHNEISTQTKEKLDSEIILLTKSLKSEGVDSGDIRSILDSYDKRFKSLKNTQNKERERQLMEMNEKIRKFKENIDKNVEKQINEKDQLFQSEN----NIVEELINIQKTLSEDDRDYILKEYNKHKISTQNSIAN 2321
            F   NK++ N  +NN N      + K++++   +  I      + L E  EKA        SL+      L NN    +ER  NL  T   Y + IE+           +L  + K+ E   M +    +      +  D  LS   ++L   ++       + E+  E IKN L+ + Q+L S++              EK    Q  +LD   QK N+ +K K+ E+ DT ++ E       +M+  L+D +E           +YE+    L  ++D +R+   +K+ +         L +   +H  E+++ LA      N+ S+  D    +  + ++ LN   +++     VNE   +  E +    E L+ + +L    LK +     DI++           L+N   K+ +  LM   +K++ +      N   +I       +  N    ++++ L  I+K L E    Y        +I+   S AN
Sbjct: 2192 FHNLNKSIENKSINNYNSSLDGSRDKANTLEKELQTI-----DSDLFELQEKA--------SLSSRKGATLENNTIATKERAQNLLDTVKNYMKDIEAAQNGSQTMDEEEL--KRKLAEVAAMLRDMRFRSTTPQGKQADYELSQANQLLDRVKSEMADRTAANEATVEEIKNKLNKFHQELTSLRDALNEAANNTALASEKNNANQ-KQLDENQQKVND-LKKKQKEVTDTMQMAEDDVAQVNDMLAMLQDSKE-----------EYER----LAAQLDGARQPLAEKVQNFSTSGAKSPLVEEAEKH-AELLDQLA-----KNLSSVISDTN--QDGFIQRALNASRAYNNIINSVNEADNLAKEANKSATEALEPKCVLNWTELKPQ---LQDIKN----------RLQNATTKKSD--LMMDLQKVQNYLNTSQGNTSGEIQAAKSAAEKANSTAADVLDTLDPIKKKLDEWQEQYGSSNMTNDEINKALSEAN 2634
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BLAST of Limbin vs. Ensembl Nematostella
Match: EDO31293 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYD8])

HSP 1 Score: 49.6766 bits (117), Expect = 8.666e-6
Identity = 41/174 (23.56%), Postives = 92/174 (52.87%), Query Frame = 3 
Query: 1887 ENSHDAEGVNEIIKIHNEISTQTKEKLDSEIILLTKSLKSEGVDSGDIRSILDSYDKRFKSLKNTQNKERERQLMEMNEKIRKFKENIDKNVEKQINEKDQLFQSENNIVEELINIQKTLSEDDRDYILKEYNKHKISTQNSIANSFLEQKRKMEEALALRKAKQLKEID-DKH 2405
            E   D E   E+ K     S +  E+  + +  + + + ++G+D  +  +I+D Y       +  + +ER++    + +++ K +  +++    +  E+ +L + E  IV +LI  Q  +SE +R+ I++E+ KH    + S+  + L Q++ +EE LA R+ +++ E++ D+H
Sbjct:  435 EQKADREAAEELEKFDEVYSHEIHEQAKTSLKKIYQDMAAKGLDEAEKNAIIDRYMTEATQQQEKREEERQKNADRVQKRLGKLRRKLEEKAILENEEQTKLRKKEEQIVTDLIVNQANMSEQERNKIIEEHEKHLAELETSMTFNKLRQRQMLEERLAARRKRRMDELERDQH 608
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BLAST of Limbin vs. Planmine SMEST
Match: SMESG000017303.1 (SMESG000017303.1)

HSP 1 Score: 2209.49 bits (5724), Expect = 0.000e+0
Identity = 1158/1168 (99.14%), Postives = 1163/1168 (99.57%), Query Frame = 3 
Query:   24 MFINLFICLVFWINFFSSQSFSVYKSNFDRREYLTGQINNIHFETIRKSVVKRSVSGPFMLTKIFKGDTKNNYNCVILFKLNSSGPEYQNSAFSLHDDISGLTVSNITSQFKDPSFSISNTGSTLEIIYLYSSNNTLEIDVRYNFQISSISSQLNKIVTFNDNRSNLLTEMACISPATFQDNVNANQXXXXXXXXXXXXXXXXXXXXXXTLWKSTSKQDKNEISSEKNSKYENYFLLVDNSQMTIRNDTQGTNEKTFLDALVQDDFRIIEHEVYLLNLNSATKTDEKIKNDFSEFNKTLFNIWLNNVNEKYPSRKSKSDSIRDGVYKIAEEINQASLKEYNEKAKKIAMESISLNKMNLEYLSNNQEEERERIMNLTSSTYKEKIESDLAKVFEHQHHDLISQLKIKENLDMEKLKLTQEELLNSQLNDLYLSAVKEILKNSENNKNLEGISAESASELIKNYLDWQQQLQSIKEKEKIRQLGRLDYIFQKRNNFIKLKENEINDTNEVMEKKNEFFVEMINNLKDREELDGRVCETILLDYEKYKKDLFKEMDTSRRDQNDKMLSLLNQKRNEKLNDLKNQHDNEIVNLLASHSADNNIKSLSDDLKNIESKYYEKVLNEENSHDAEGVNEIIKIHNEISTQTKEKLDSEIILLTKSLKSEGVDSGDIRSILDSYDKRFKSLKNTQNKERERQLMEMNEKIRKFKENIDKNVEKQINEKDQLFQSENNIVEELINIQKTLSEDDRDYILKEYNKHKISTQNSIANSFLEQKRKMEEALALRKAKQLKEIDDKHDLKVEELINRKMRVKTKRTQQEIKEESEKELMLDYVNQKLQLSSVGNSKDDLDKELEIIRNELLAERAALVKKKEEEYVALILKVQMLKAKESAELKDQEEKIFKLQLAALTSSKAQNSQEIFKVYQNTKENLKIELDKNKEIQESTLKEKLENRKRNTLRKKTENEPANDDSETIRRKQQSIEDQKLKNEINMERSIQNVEKDMLKKCISSSDCSREEVFNLMLEIYPLKSSQEIEELVEEFCPKSRQPPQNNQLKSGITLLEAYRKHNEKHKTKASDDSVKIPKISQRNEKNISNYNEKVKVSNEFPNSYDENDGDFVDINEEKIHFKIENKNFAENISNENAENXXXXXXXXXXXXXLDDQENIDDNLEQF 3527
            MFINLFICLVFWINFFSSQSFSVYKSNFDRREYLTGQINNIHFETIRKSVVKRSVSGPFMLTKIFKGDTKNNYNCVILFKLNSSGPEYQNSAFSLHDDISGLTVSNITSQFKDPSFSISNTGSTLEIIYLYSSNNTLEIDVRYNFQISSISSQLNKIVTFNDNRSNLLTE ACISPATFQDNVNANQLVILFIILGLVLILLLFFIIIITLWKSTSKQDKNEISSEKNSKYENYFLLVDNSQMTIRNDTQGTNEKTFLDALVQDDFRIIEHEVYLLNLNSATKTDEKIKNDFSEFNKTLFNIWLNNVNEKYPSRKSKSDSIRDGVYKIAEEINQASLKEYNEKAKKIAMESISLNKMNLEYLS NQEEERERIMNLTSSTYKEKIESDLAKVFEHQ HDLISQLKIKENLDMEKLKLTQEELLNSQLNDL+LSAVKEILKNSENNKNLEGISAESASELIKNYLDWQQQLQSIKEKEKIRQLGRLDYIFQKRNNFIKLKENEINDTNEV+EKKNEFFVEMINNLKDREELDGRVCETILLDYEKYKKDLFKEMDTSRRDQNDKMLSLLNQKRNEKLNDLKNQHDNEIVNLL SHSADNNIKSLSDDL NIESKYYEKVLNEENSHDAEGVNEIIKIHNEISTQTKEKLDSEIILLTKSLKSEGVDSGDIRSILDSYDKRFKSLKNTQNKERERQLMEMNEKIRKFKENIDKNVEKQINEKDQLFQSENNIVEEL+NIQKTLSEDDRDYILKEYNKHKISTQNSIANSFLEQKRKMEEALALRKAKQLKEIDDKHDLKVEELINRKMRVKTKRTQQEIKEESEKELMLDYVNQKLQ+SSVGNSKDDLDKELEIIRNELLAERAALVKKKEEEYVALILKVQMLKAKESAELK+QEEKIFKLQLAALTSSKAQNSQEIFKVYQNTKENLKIELDKNKEIQESTLKEKLENRKRNTLRKKTENEPANDDSETIRRKQQSIEDQKLKNEINMERSIQNVEKDMLKKCISSSDCSREEVFNLMLEIYPLKSSQEIEELVEEFCPKSRQPPQNNQLKSGITLLEAYRKHNEKHKTKASDDSVKIPKISQRNEKNISNYNEKVKVSNEFPNSYDENDGDFVDINEEKIHFKIENKNFAENISNENAENKGRKRKKKSRRKKLDDQENIDDNLEQF
Sbjct:    1 MFINLFICLVFWINFFSSQSFSVYKSNFDRREYLTGQINNIHFETIRKSVVKRSVSGPFMLTKIFKGDTKNNYNCVILFKLNSSGPEYQNSAFSLHDDISGLTVSNITSQFKDPSFSISNTGSTLEIIYLYSSNNTLEIDVRYNFQISSISSQLNKIVTFNDNRSNLLTETACISPATFQDNVNANQLVILFIILGLVLILLLFFIIIITLWKSTSKQDKNEISSEKNSKYENYFLLVDNSQMTIRNDTQGTNEKTFLDALVQDDFRIIEHEVYLLNLNSATKTDEKIKNDFSEFNKTLFNIWLNNVNEKYPSRKSKSDSIRDGVYKIAEEINQASLKEYNEKAKKIAMESISLNKMNLEYLSKNQEEERERIMNLTSSTYKEKIESDLAKVFEHQRHDLISQLKIKENLDMEKLKLTQEELLNSQLNDLFLSAVKEILKNSENNKNLEGISAESASELIKNYLDWQQQLQSIKEKEKIRQLGRLDYIFQKRNNFIKLKENEINDTNEVIEKKNEFFVEMINNLKDREELDGRVCETILLDYEKYKKDLFKEMDTSRRDQNDKMLSLLNQKRNEKLNDLKNQHDNEIVNLLTSHSADNNIKSLSDDLINIESKYYEKVLNEENSHDAEGVNEIIKIHNEISTQTKEKLDSEIILLTKSLKSEGVDSGDIRSILDSYDKRFKSLKNTQNKERERQLMEMNEKIRKFKENIDKNVEKQINEKDQLFQSENNIVEELMNIQKTLSEDDRDYILKEYNKHKISTQNSIANSFLEQKRKMEEALALRKAKQLKEIDDKHDLKVEELINRKMRVKTKRTQQEIKEESEKELMLDYVNQKLQVSSVGNSKDDLDKELEIIRNELLAERAALVKKKEEEYVALILKVQMLKAKESAELKEQEEKIFKLQLAALTSSKAQNSQEIFKVYQNTKENLKIELDKNKEIQESTLKEKLENRKRNTLRKKTENEPANDDSETIRRKQQSIEDQKLKNEINMERSIQNVEKDMLKKCISSSDCSREEVFNLMLEIYPLKSSQEIEELVEEFCPKSRQPPQNNQLKSGITLLEAYRKHNEKHKTKASDDSVKIPKISQRNEKNISNYNEKVKVSNEFPNSYDENDGDFVDINEEKIHFKIENKNFAENISNENAENKGRKRKKKSRRKKLDDQENIDDNLEQF 1168
The following BLAST results are available for this feature:
BLAST of Limbin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Limbin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Limbin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Limbin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Limbin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Limbin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Limbin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Limbin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A210Q5493.960e-2523.95Limbin OS=Mizuhopecten yessoensis OX=6573 GN=KP79_... [more]
A0A1S3HPJ51.659e-2323.40trichohyalin-like OS=Lingula unguis OX=7574 GN=LOC... [more]
A0A2C9M4A73.778e-2325.62Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A433U9J52.029e-2225.76Uncharacterized protein (Fragment) OS=Elysia chlor... [more]
K1PHU89.013e-2224.30Limbin OS=Crassostrea gigas OX=29159 GN=CGI_100045... [more]
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BLAST of Limbin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
lama54.829e-721.69laminin, alpha 5 [Source:ZFIN;Acc:ZDB-GENE-030131-... [more]
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BLAST of Limbin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Limbin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Limbin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO312938.666e-623.56Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Limbin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Limbin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000017303.10.000e+099.14SMESG000017303.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013159 ID=SMED30013159|Name=Limbin|organism=Schmidtea mediterranea sexual|type=transcript|length=3547bp
TTTTCTAATAAATTTTAAAAAAAATGTTTATTAATTTATTCATCTGTCTT
GTTTTTTGGATTAATTTTTTTTCAAGTCAAAGTTTTAGTGTTTATAAATC
AAATTTTGACAGAAGAGAATATCTGACTGGTCAAATAAATAATATTCACT
TTGAAACAATTAGGAAATCAGTAGTTAAGAGAAGTGTTTCTGGCCCTTTT
ATGTTGACTAAAATTTTTAAAGGTGACACAAAAAACAATTACAATTGTGT
TATTTTATTCAAACTCAATTCCTCTGGTCCTGAATATCAAAATTCTGCAT
TCAGTCTTCATGATGATATTAGTGGATTGACTGTCAGCAATATAACCTCC
CAATTCAAAGACCCTTCTTTCTCAATTTCAAACACCGGATCAACACTTGA
AATCATTTATCTTTATTCCAGCAATAACACGTTAGAAATTGATGTACGAT
ATAATTTTCAAATCAGTTCGATTTCATCTCAGTTAAACAAAATTGTGACT
TTTAATGACAATCGGAGCAATTTACTAACTGAAATGGCTTGCATCTCACC
AGCTACCTTTCAAGATAACGTCAATGCCAACCAACTTGTCATTTTGTTCA
TTATTCTCGGATTGGTTCTGATCTTACTATTGTTTTTTATAATAATAATC
ACTTTATGGAAAAGTACATCTAAGCAGGATAAAAATGAAATTTCTTCCGA
AAAAAATTCAAAATATGAGAATTATTTCCTTTTGGTGGATAATTCTCAGA
TGACTATAAGAAATGATACCCAAGGAACCAACGAAAAAACCTTCCTTGAT
GCTTTGGTCCAGGATGATTTTCGAATTATCGAGCACGAAGTTTACCTTTT
GAATTTGAATTCTGCTACCAAAACCGACGAGAAAATAAAAAATGATTTCA
GTGAATTTAATAAAACTCTGTTTAATATTTGGCTTAACAATGTCAATGAG
AAATATCCCAGTCGGAAATCAAAATCCGATAGCATTAGGGACGGGGTTTA
TAAAATAGCAGAAGAAATAAATCAAGCCAGTCTAAAGGAATATAATGAGA
AAGCCAAGAAAATTGCCATGGAATCAATATCTTTAAACAAGATGAATTTG
GAATATTTATCAAATAACCAAGAAGAGGAAAGAGAAAGGATAATGAATCT
TACTTCGTCAACATATAAAGAAAAAATTGAAAGTGATTTGGCCAAAGTCT
TCGAACATCAGCACCATGATCTCATTTCCCAATTGAAAATTAAAGAAAAT
TTAGATATGGAAAAATTGAAATTGACTCAAGAAGAATTGCTAAACTCTCA
ATTAAACGATCTCTATTTATCGGCAGTGAAAGAAATTCTAAAAAATAGTG
AAAATAATAAAAACCTTGAAGGGATTTCCGCAGAAAGTGCGAGTGAATTG
ATAAAAAATTATCTAGATTGGCAACAGCAGCTTCAATCCATTAAAGAGAA
GGAAAAAATTCGTCAATTAGGACGACTTGATTATATTTTTCAAAAACGAA
ATAATTTTATAAAACTAAAAGAAAATGAAATAAATGATACAAATGAAGTG
ATGGAAAAGAAAAACGAATTCTTTGTCGAAATGATTAACAATTTAAAGGA
TCGAGAAGAACTTGATGGTCGAGTTTGTGAAACAATACTATTGGATTATG
AAAAGTATAAGAAAGATTTATTTAAAGAAATGGACACTTCTAGGAGAGAT
CAAAATGATAAGATGCTAAGTTTATTGAATCAAAAAAGGAATGAAAAACT
AAATGATTTGAAAAACCAACATGACAACGAAATTGTCAATTTATTGGCAT
CTCATAGTGCTGATAATAATATCAAATCGTTGAGTGATGATCTCAAAAAT
ATCGAGAGTAAATATTATGAAAAAGTTCTTAATGAAGAAAATAGTCATGA
TGCAGAGGGGGTAAATGAAATTATAAAGATTCACAATGAAATATCAACTC
AAACGAAAGAAAAATTAGATTCGGAAATTATTCTATTAACTAAAAGTCTT
AAAAGTGAAGGTGTTGATTCTGGAGATATTAGAAGCATTCTTGATAGTTA
CGATAAAAGATTCAAAAGTTTGAAAAATACTCAAAATAAAGAGAGAGAAC
GACAGTTGATGGAAATGAATGAAAAGATACGAAAATTTAAAGAAAATATT
GATAAAAATGTGGAAAAACAAATAAATGAAAAAGACCAATTATTTCAATC
GGAAAACAACATCGTCGAAGAATTGATAAATATACAAAAAACTTTATCTG
AAGACGACAGAGATTATATTTTGAAGGAATATAACAAACACAAAATATCA
ACCCAGAATAGTATAGCTAACTCTTTTCTGGAACAAAAAAGGAAAATGGA
AGAAGCTTTGGCATTGAGAAAAGCTAAACAGTTAAAGGAGATTGATGATA
AACATGATTTGAAAGTTGAGGAGTTAATCAATAGAAAAATGCGGGTTAAA
ACAAAAAGAACTCAGCAGGAAATCAAGGAAGAATCTGAGAAGGAACTTAT
GCTTGATTATGTGAATCAAAAATTACAATTGTCGTCAGTTGGTAATTCGA
AAGATGATCTGGACAAAGAGTTGGAGATAATTCGTAATGAATTACTTGCT
GAAAGAGCAGCTCTTGTGAAGAAAAAGGAAGAAGAATACGTTGCTCTGAT
TTTAAAAGTTCAAATGTTAAAAGCTAAAGAAAGTGCGGAATTGAAAGACC
AAGAAGAGAAGATTTTCAAACTTCAATTAGCAGCTTTGACGAGCAGTAAA
GCGCAAAACTCACAGGAAATTTTCAAAGTCTATCAAAATACAAAAGAAAA
TCTTAAAATTGAGCTCGATAAAAATAAAGAAATTCAAGAATCAACATTAA
AAGAGAAATTAGAGAATCGGAAAAGAAATACGTTGAGAAAGAAAACAGAA
AATGAACCTGCAAATGATGACAGTGAAACGATAAGACGCAAGCAGCAGTC
TATTGAAGATCAAAAATTAAAAAACGAAATCAACATGGAGCGTTCTATTC
AAAACGTTGAAAAGGACATGTTAAAAAAATGCATTAGTTCGTCAGATTGT
TCTCGTGAAGAAGTTTTTAATTTGATGTTGGAAATCTATCCTTTAAAGTC
CAGTCAAGAGATTGAGGAATTGGTCGAAGAGTTTTGCCCAAAATCCAGAC
AACCTCCTCAAAATAATCAACTCAAATCAGGGATAACTCTATTGGAAGCC
TATAGAAAACACAACGAAAAACATAAAACAAAAGCAAGTGATGATTCTGT
CAAAATTCCAAAAATTTCACAAAGAAATGAAAAAAACATAAGCAATTACA
ATGAAAAAGTAAAGGTTTCGAATGAATTTCCTAATTCGTATGACGAAAAT
GACGGTGATTTTGTTGATATTAATGAAGAGAAAATACATTTCAAGATAGA
AAATAAGAATTTTGCAGAAAATATATCGAACGAAAATGCTGAGAATAAAG
GTCGAAAAAGAAAAAAGAAATCTCGTCGAAAGAAATTAGATGACCAGGAA
AATATTGATGACAATTTGGAACAATTTTAGATTAATTTATTTGCTAG
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protein sequence of SMED30013159-orf-1

>SMED30013159-orf-1 ID=SMED30013159-orf-1|Name=SMED30013159-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1169bp
MFINLFICLVFWINFFSSQSFSVYKSNFDRREYLTGQINNIHFETIRKSV
VKRSVSGPFMLTKIFKGDTKNNYNCVILFKLNSSGPEYQNSAFSLHDDIS
GLTVSNITSQFKDPSFSISNTGSTLEIIYLYSSNNTLEIDVRYNFQISSI
SSQLNKIVTFNDNRSNLLTEMACISPATFQDNVNANQLVILFIILGLVLI
LLLFFIIIITLWKSTSKQDKNEISSEKNSKYENYFLLVDNSQMTIRNDTQ
GTNEKTFLDALVQDDFRIIEHEVYLLNLNSATKTDEKIKNDFSEFNKTLF
NIWLNNVNEKYPSRKSKSDSIRDGVYKIAEEINQASLKEYNEKAKKIAME
SISLNKMNLEYLSNNQEEERERIMNLTSSTYKEKIESDLAKVFEHQHHDL
ISQLKIKENLDMEKLKLTQEELLNSQLNDLYLSAVKEILKNSENNKNLEG
ISAESASELIKNYLDWQQQLQSIKEKEKIRQLGRLDYIFQKRNNFIKLKE
NEINDTNEVMEKKNEFFVEMINNLKDREELDGRVCETILLDYEKYKKDLF
KEMDTSRRDQNDKMLSLLNQKRNEKLNDLKNQHDNEIVNLLASHSADNNI
KSLSDDLKNIESKYYEKVLNEENSHDAEGVNEIIKIHNEISTQTKEKLDS
EIILLTKSLKSEGVDSGDIRSILDSYDKRFKSLKNTQNKERERQLMEMNE
KIRKFKENIDKNVEKQINEKDQLFQSENNIVEELINIQKTLSEDDRDYIL
KEYNKHKISTQNSIANSFLEQKRKMEEALALRKAKQLKEIDDKHDLKVEE
LINRKMRVKTKRTQQEIKEESEKELMLDYVNQKLQLSSVGNSKDDLDKEL
EIIRNELLAERAALVKKKEEEYVALILKVQMLKAKESAELKDQEEKIFKL
QLAALTSSKAQNSQEIFKVYQNTKENLKIELDKNKEIQESTLKEKLENRK
RNTLRKKTENEPANDDSETIRRKQQSIEDQKLKNEINMERSIQNVEKDML
KKCISSSDCSREEVFNLMLEIYPLKSSQEIEELVEEFCPKSRQPPQNNQL
KSGITLLEAYRKHNEKHKTKASDDSVKIPKISQRNEKNISNYNEKVKVSN
EFPNSYDENDGDFVDINEEKIHFKIENKNFAENISNENAENKGRKRKKKS
RRKKLDDQENIDDNLEQF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: biological process
TermDefinition
GO:0007224smoothened signaling pathway
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane