MIEAP domain-containing protein

Overview


Name	MIEAP domain-containing protein
Smed ID	SMED30013149
Length (bp)	1668

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of MIEAP domain-containing protein (SMED30013149) t-SNE clustered cells

Violin plots show distribution of expression levels for MIEAP domain-containing protein (SMED30013149) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of MIEAP domain-containing protein (SMED30013149) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for MIEAP domain-containing protein (SMED30013149) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30013149

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 10

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
parapharyngeal region	SMED30013149	h1SMcG0001291	SmedASXL_011076	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pharynx	SMED30013149	h1SMcG0001291	dd_Smed_v4_6321_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30013149	h1SMcG0001291	dd_Smed_v4_6321_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30013149	h1SMcG0001291	dd_Smed_v4_6321_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
head region	SMED30013149	h1SMcG0001291	dd_Smed_v6_6321_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
pharynx	SMED30013149	h1SMcG0001291	dd_Smed_v6_6321_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
epidermis	SMED30013149	h1SMcG0001291	dd_Smed_v4_6321_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
ventral epidermis	SMED30013149	h1SMcG0001291	dd_Smed_v4_6321_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
ventral epidermis	SMED30013149	h1SMcG0001291	dd_Smed_v4_6321_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
ciliated epithelial cell	SMED30013149	h1SMcG0001291	dd_Smed_v4_6321_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Human
Match: SPATA18 (spermatogenesis associated 18 [Source:HGNC Symbol;Acc:HGNC:29579])

HSP 1 Score: 214.157 bits (544), Expect = 4.327e-62
Identity = 163/493 (33.06%), Postives = 258/493 (52.33%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGF--FSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIK--------------SEN----------------NLLKTKAYKTDS-----YKHEIEHLKDDVAILASHRNP----HCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICH-----GKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLI-GHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M+E L+RLV+  +   LQ +L+ WL  Y  NTCDQN+    E+IE  AKVQGQLF +L+  A EGG   G   IK+RLLP L   F   S G S DS +  L+DT   +++     R  ++ +  L+++LN TR + N ++ +L E +  LE   ++SA   +  ++EI  LK QL+S++              SEN                N LK  A + D+     YK ++ +LK+++A+L++ ++              +P     S          + V    P         +   L+++ +D +S +RLDA   L R  D  E  Q+II+ + +E+F+ AK AFR +K  VR  L   +  S   E++V  Y+  H D+  D  S   +VIR+++   P I  P  + F ++  FI+E C IA+ M AL  P+D+ +  ++GE++++ +YRRSYDS+++A  + +HVWP L ++  VI KGEAVT
Sbjct:    1 MAENLKRLVSNETLRTLQEKLDFWLKEYNTNTCDQNLNHCLELIEQVAKVQGQLFGILTAAAQEGGRNDGVETIKSRLLPWLEASFTAASLGKSVDSKVPSLQDTFDRERHKDPSPR--DRDMQQLDSNLNSTRSQCNQVQDDLVETEKNLEESKNRSAISLLAAEEEINQLKKQLKSLQAQEDARHRNTDQRSSENRRSEPWSLEERKREQWNSLKQNADQQDTEAMSDYKKQLRNLKEEIAVLSAEKSALQGRSSRSRSPSPAPRSRSCSRSRSASPSTAVKVRRPSPNRSKLSNVARKAALLSRFSDSYSQARLDAQCLLRRCIDKAETVQRIIYIATVEAFHVAKMAFRHFKIHVRKSLTPSYVGSNDFENAVLDYVICHLDL-YDSQSSVNDVIRAMNVN-PKISFPPVVDFCLLSDFIQEICCIAFAMQALEPPLDIAY-GADGEVFNDCKYRRSYDSDFTAPLVLYHVWPALMENDCVIMKGEAVT 488

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Human
Match: SPATA18 (spermatogenesis associated 18 [Source:HGNC Symbol;Acc:HGNC:29579])

HSP 1 Score: 213.772 bits (543), Expect = 9.388e-62
Identity = 163/493 (33.06%), Postives = 258/493 (52.33%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGF--FSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIK--------------SEN----------------NLLKTKAYKTDS-----YKHEIEHLKDDVAILASHRNP----HCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICH-----GKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLI-GHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M+E L+RLV+  +   LQ +L+ WL  Y  NTCDQN+    E+IE  AKVQGQLF +L+  A EGG   G   IK+RLLP L   F   S G S DS +  L+DT   +++     R  ++ +  L+++LN TR + N ++ +L E +  LE   ++SA   +  ++EI  LK QL+S++              SEN                N LK  A + D+     YK ++ +LK+++A+L++ ++              +P     S          + V    P         +   L+++ +D +S +RLDA   L R  D  E  Q+II+ + +E+F+ AK AFR +K  VR  L   +  S   E++V  Y+  H D+  D  S   +VIR+++   P I  P  + F ++  FI+E C IA+ M AL  P+D+ +  ++GE++++ +YRRSYDS+++A  + +HVWP L ++  VI KGEAVT
Sbjct:    1 MAENLKRLVSNETLRTLQEKLDFWLKEYNTNTCDQNLNHCLELIEQVAKVQGQLFGILTAAAQEGGRNDGVETIKSRLLPWLEASFTAASLGKSVDSKVPSLQDTFDRERHKDPSPR--DRDMQQLDSNLNSTRSQCNQVQDDLVETEKNLEESKNRSAISLLAAEEEINQLKKQLKSLQAQEDARHRNTDQRSSENRRSEPWSLEERKREQWNSLKQNADQQDTEAMSDYKKQLRNLKEEIAVLSAEKSALQGRSSRSRSPSPAPRSRSCSRSRSASPSTAVKVRRPSPNRSKLSNVARKAALLSRFSDSYSQARLDAQCLLRRCIDKAETVQRIIYIATVEAFHVAKMAFRHFKIHVRKSLTPSYVGSNDFENAVLDYVICHLDL-YDSQSSVNDVIRAMNVN-PKISFPPVVDFCLLSDFIQEICCIAFAMQALEPPLDIAY-GADGEVFNDCKYRRSYDSDFTAPLVLYHVWPALMENDCVIMKGEAVT 488

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Human
Match: SPATA18 (spermatogenesis associated 18 [Source:HGNC Symbol;Acc:HGNC:29579])

HSP 1 Score: 211.46 bits (537), Expect = 3.545e-61
Identity = 156/467 (33.40%), Postives = 250/467 (53.53%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGF--FSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQ---LESIKSEN-NLLKTKAYKTDS-----YKHEIEHLKDDVAILASHRNP----HCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICH-----GKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLI-GHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M+E L+RLV+  +   LQ +L+ WL  Y  NTCDQN+    E+IE  AKVQGQLF +L+  A EGG   G   IK+RLLP L   F   S G S DS +  L+DT   +++     R  ++ +  L+++LN TR + N ++ EL+        Q  + A  +  D++   + +++   LE  K E  N LK  A + D+     YK ++ +LK+++A+L++ ++              +P     S          + V    P         +   L+++ +D +S +RLDA   L R  D  E  Q+II+ + +E+F+ AK AFR +K  VR  L   +  S   E++V  Y+  H D+  D  S   +VIR+++   P I  P  + F ++  FI+E C IA+ M AL  P+D+ +  ++GE++++ +YRRSYDS+++A  + +HVWP L ++  VI KGEAVT
Sbjct:    1 MAENLKRLVSNETLRTLQEKLDFWLKEYNTNTCDQNLNHCLELIEQVAKVQGQLFGILTAAAQEGGRNDGVETIKSRLLPWLEASFTAASLGKSVDSKVPSLQDTFDRERHKDPSPR--DRDMQQLDSNLNSTRSQCNQVQDELKSL------QAQEDARHRNTDQRSSENRRSEPWSLEERKREQWNSLKQNADQQDTEAMSDYKKQLRNLKEEIAVLSAEKSALQGRSSRSRSPSPAPRSRSCSRSRSASPSTAVKVRRPSPNRSKLSNVARKAALLSRFSDSYSQARLDAQCLLRRCIDKAETVQRIIYIATVEAFHVAKMAFRHFKIHVRKSLTPSYVGSNDFENAVLDYVICHLDL-YDSQSSVNDVIRAMNVN-PKISFPPVVDFCLLSDFIQEICCIAFAMQALEPPLDIAY-GADGEVFNDCKYRRSYDSDFTAPLVLYHVWPALMENDCVIMKGEAVT 456

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Human
Match: SPATA18 (spermatogenesis associated 18 [Source:HGNC Symbol;Acc:HGNC:29579])

HSP 1 Score: 86.6557 bits (213), Expect = 4.906e-20
Identity = 49/103 (47.57%), Postives = 62/103 (60.19%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFF--STGLSSDSSLKVLE 362
            M+E L+RLV+  +   LQ +L+ WL  Y  NTCDQN+    E+IE  AKVQGQLF +L+  A EGG   G   IK+RLLP L   F   S G S DS +  L+
Sbjct:    1 MAENLKRLVSNETLRTLQEKLDFWLKEYNTNTCDQNLNHCLELIEQVAKVQGQLFGILTAAAQEGGRNDGVETIKSRLLPWLEASFTAASLGKSVDSKVPSLQ 103

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Zebrafish
Match: spata18 (spermatogenesis associated 18 [Source:NCBI gene;Acc:497395])

HSP 1 Score: 277.33 bits (708), Expect = 1.395e-86
Identity = 166/449 (36.97%), Postives = 268/449 (59.69%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFF--STGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRNPHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLIG-HSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M++ LRRLVN+  +++LQ +L+ W  +Y   +CDQN+ R CE++EL +K+QGQLF +L+L A EGG Y G   +K+RLLP LG  F   ++ +SSD+SL +++++    + ++E+  ++E +L  +E  L  TR E +++K EL +  +EL+   ++SA+  +  + EI  LK++L   + + +L K K    D Y+ ++  L+D+V+ L + +           SP  P       +                 ++ +LV++ +DL++  RL++ + L +Y DD E  Q+I+F + +ESF AAK A+R +KSRVR  L   H    SLED+V  YI  + D+  DV +   +VI +++   P I  P  + F +I SFIREACRIA+ M  L  P+D+   +S+GELY + +YRRS+DSE++A  ++ HVWP L +   VI KGEAVT
Sbjct:    1 MADTLRRLVNSSPYSVLQDKLDAWYKDYHVYSCDQNLNRCCELVELTSKIQGQLFTILNLTAQEGGHYSGVDTLKSRLLPWLGSCFTIAASAVSSDTSLSLIQESVEKDRKIRELSSVHESNLQKIEDQLCSTRIELDSVKRELVDTHIELDSTKNKSATTLLATEDEILHLKSELRVAQDQLDLYKRKLDVLDDYERQVRILRDEVSFLNAEKTVLQDRLARSRSPS-PLLRRSRSVSPVRGESPTRAQLTSSSRHARLVSRFSDLYATERLESQSLLLKYIDDLETVQRILFIAAVESFQAAKLAYRQFKSRVRKTLSSTHIGPESLEDAVVDYIVRNLDL-YDVQTSINDVINAMNVN-PRISFPPEVDFVLISSFIREACRIAFAMQTLDPPLDLAF-SSDGELYSDVKYRRSFDSEFTAPLVAFHVWPALLEGDTVILKGEAVT 445

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000051448.1 (spermatogenesis associated 18 [Source:NCBI gene;Acc:100379837])

HSP 1 Score: 260.381 bits (664), Expect = 1.044e-79
Identity = 168/450 (37.33%), Postives = 261/450 (58.00%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGF--FSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRNPHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICHGKYQ-QLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDL-IGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M++ LRRL N+    +LQ +L+ W  +Y  N+CD N+   CE++ELN+K+QGQLFK+LS+ A EGG Y G   +K+R LP LG  F   S G  S++S  +L ++   K +       +E+ L+++E+ L+ TR E N+++ EL E +++LE   ++SA+  +  ++EI  L+ +L + + +  L    +   D Y+ +I  L+D+++IL++  N              P                  C  I +   Q +L+++ ND+F+  RLDA T L RY DD E  Q+IIF + +ESF+AAK AFR ++ RVR  L   H    SLED+V  YI  + D+  DV S   EVI +++   P I  P  + F +I +FIRE CR+A+ M  L  P+D+   T +GEL+ E +YRR+YDSE++A  + +HVWP L ++  VI KGEA+T
Sbjct:   25 MADTLRRLANSEKCRVLQDKLDDWYKDYHINSCDSNLNVCCELLELNSKIQGQLFKILSVTAREGGQYAGVETLKSRFLPWLGTCFSASSPGSFSENSCSILNESMERKLSAS-----HERELNEVESKLSSTRIELNSVRQELLETQMDLEDTKNKSANTLLATEEEILQLRAELRAAREKLELRSLDS--IDDYEKQIRLLRDEISILSAE-NGILNSRVSRSRSPSPIRHSSRSSSPFPRSESPTCAKITNASRQARLISRFNDIFANDRLDAQTLLRRYIDDLEMVQRIIFIATVESFHAAKMAFRQFRLRVRKSLSPSHMGPESLEDTVIDYIVRNLDL-YDVQSSVNEVISAMNVN-PKISFPPDVDFILISAFIREVCRVAFAMQTLDPPLDIAF-TVDGELFTESKYRRTYDSEHTAPLVYYHVWPALMENDNVIVKGEALT 463

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Xenopus
Match: itga8 (integrin subunit alpha 8 [Source:Xenbase;Acc:XB-GENE-961825])

HSP 1 Score: 248.44 bits (633), Expect = 1.988e-75
Identity = 164/449 (36.53%), Postives = 258/449 (57.46%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGF--FSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRNPHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLIGHSHSC-SLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M++ LRRL N+    +LQ +L++W  +Y  N+CD N+   CE++ELN+ VQGQLF++LS+ A EGG Y G   IK+R LP LG  F   S G  S++S  +L     N    +++   +E+ L ++E+ L+ TR + N+++ EL E +++LE   ++SA+  +  ++EI  L+ +L   + + +L    +   D Y+ +I  LKD+++IL+S  +          SP    HS         +            +  +L+ + N++F+  RLDA T L RY +D +  Q+IIF + +ESF AAK AFR ++ RVR  L        SLED+V  YI  + D+  DV S   EVI +++   P I  P  + F +I  FIRE CR+A+ M  L  P+D+   T++GEL+ + +YRR+YDSE++A  + +HVWP L ++  VI KGEAVT
Sbjct:    1 MADTLRRLANSEKCRVLQDKLDNWYKDYHINSCDSNLNVCCELLELNSNVQGQLFQILSVTAREGGQYAGVETIKSRFLPWLGTCFSTSSPGSFSENSFAIL-----NHSMQRKLSTSHERELIEVESKLSSTRIDLNSVRQELLETQMDLEDTKTKSANTLLATEEEILQLRAELRVAREKLDLKSLDS--IDDYERQIRLLKDEISILSSENSILKSRLSRSRSPSPIRHSSRSSSPFTRSESPTSAKLTSASRQARLIFRFNNIFANDRLDAQTLLRRYIEDLDMVQRIIFIATVESFRAAKMAFRQFRLRVRKSLSPSQMGPESLEDAVIDYIVRNLDL-FDVQSSVNEVIGAMNVN-PKISFPPEVDFILISGFIREVCRVAFAMQTLDPPLDIAF-TADGELFTDSKYRRTYDSEHTAPLVYYHVWPALMENDNVIVKGEAVT 439

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000051452.1 (spermatogenesis associated 18 [Source:NCBI gene;Acc:100379837])

HSP 1 Score: 245.743 bits (626), Expect = 1.578e-74
Identity = 163/452 (36.06%), Postives = 242/452 (53.54%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFF--STGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIK--SENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRNPHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICHGKYQ-QLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDL-IGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M++ LRRL N+    +LQ +L+ W  +Y  N+CD N+   CE++ELN+K+QGQLFK+LS+ A EGG Y G   +K+R LP LG  F   S G  S++S  +L ++   K +       +E+ L+++E+ L+ TR E N++                         +QE+ + +  LE  K  S N LL T+         EI  L+ +V+     R+P  +                             C  I +   Q +L+++ ND+F+  RLDA T L RY DD E  Q+IIF + +ESF+AAK AFR ++ RVR  L   H    SLED+V  YI  + D+  DV S   EVI +++   P I  P  + F +I +FIRE CR+A+ M  L  P+D+   T +GEL+ E +YRR+YDSE++A  + +HVWP L ++  VI KGEA+T
Sbjct:   25 MADTLRRLANSEKCRVLQDKLDDWYKDYHINSCDSNLNVCCELLELNSKIQGQLFKILSVTAREGGQYAGVETLKSRFLPWLGTCFSASSPGSFSENSCSILNESMERKLSAS-----HERELNEVESKLSSTRIELNSV-------------------------RQELLETQMDLEDTKNKSANTLLATE--------EEILQLRAEVS---RSRSPSPIRH------------SSRSSSPFPRSESPTCAKITNASRQARLISRFNDIFANDRLDAQTLLRRYIDDLEMVQRIIFIATVESFHAAKMAFRQFRLRVRKSLSPSHMGPESLEDTVIDYIVRNLDL-YDVQSSVNEVISAMNVN-PKISFPPDVDFILISAFIREVCRVAFAMQTLDPPLDIAF-TVDGELFTESKYRRTYDSEHTAPLVYYHVWPALMENDNVIVKGEALT 420

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Xenopus
Match: itga8 (integrin subunit alpha 8 [Source:Xenbase;Acc:XB-GENE-961825])

HSP 1 Score: 245.358 bits (625), Expect = 2.695e-74
Identity = 163/461 (35.36%), Postives = 257/461 (55.75%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGF--FSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRN------------PHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLIGHSHSC-SLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M++ LRRL N+    +LQ +L++W  +Y  N+CD N+   CE++ELN+ VQGQLF++LS+ A EGG Y G   IK+R LP LG  F   S G  S++S   ++         +++   +E+ L ++E+ L+ TR + N+++ EL E +++LE   ++SA+  +  ++EI  L+ +L   + + +L    +   D Y+ +I  LKD+++IL+S  +                HS    SP     S                      +  +L+ + N++F+  RLDA T L RY +D +  Q+IIF + +ESF AAK AFR ++ RVR  L        SLED+V  YI  + D+  DV S   EVI +++   P I  P  + F +I  FIRE CR+A+ M  L  P+D+   T++GEL+ + +YRR+YDSE++A  + +HVWP L ++  VI KGEAVT
Sbjct:    1 MADTLRRLANSEKCRVLQDKLDNWYKDYHINSCDSNLNVCCELLELNSNVQGQLFQILSVTAREGGQYAGVETIKSRFLPWLGTCFSTSSPGSFSENSFHSMQ---------RKLSTSHERELIEVESKLSSTRIDLNSVRQELLETQMDLEDTKTKSANTLLATEEEILQLRAELRVAREKLDLKSLDS--IDDYERQIRLLKDEISILSSENSILKSRLSRSRSPSPIRHSSRSSSPFTRSESPTS------------AKLTSASRQARLIFRFNNIFANDRLDAQTLLRRYIEDLDMVQRIIFIATVESFRAAKMAFRQFRLRVRKSLSPSQMGPESLEDAVIDYIVRNLDL-FDVQSSVNEVIGAMNVN-PKISFPPEVDFILISGFIREVCRVAFAMQTLDPPLDIAF-TADGELFTDSKYRRTYDSEHTAPLVYYHVWPALMENDNVIVKGEAVT 435

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Mouse
Match: Spata18 (spermatogenesis associated 18 [Source:MGI Symbol;Acc:MGI:1920722])

HSP 1 Score: 196.823 bits (499), Expect = 1.594e-55
Identity = 150/496 (30.24%), Postives = 256/496 (51.61%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFST--GLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSAS--DKMFDKQEIRDLKTQLESIKSENNLL-------KTKAYKTD-----------------SYKHEIEHLKDDVAILASHRNPHCLHSPICHSPCDPCHSHGCLL-------------DSCCTLHVDHCDPICH-------GKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLIGHS--HSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M+E L++L  + S   LQ ++ +W+++Y  N+CDQN+    E+IE  AKVQ QLF +L++ A EGG   G   IK RLLP+L   F S   G +++S +   +D     KN     R      D  ++D N +  E    +++ + A+     +G+++ S    +  ++EI  LK QL+S++++ +         +++A K+D                 +Y+  +++LK+++A+L++ ++     S    SP     SH                 +S CT       P  +        +   L+++ +D +S +RLDA   L R  D  E  Q+II+ + +E+F+ AK AFR +K RVR  L   +   +   E +V  YI  H D+  D  S   +VIR+++   P I  P  + F ++  FI+E C IA+ M +L  P+D+    ++GE++++ +YRRSYDS+++A  + +HVWP L ++  VI KGEAVT
Sbjct:    1 MAESLKKLAKSESLQALQDKVTYWVNDYNSNSCDQNLNYCIELIEQVAKVQAQLFGILTVTAQEGGNNEGVETIKCRLLPLLQTSFSSVNMGKTAESEMCATQDFQLRSKN-----RDNSPDQDQHQSD-NESFSETQPTQVQDDLAESGKSLEGAKNGSTISLLAAEEEINQLKKQLKSLQAQEDARHKTSENRRSEALKSDHRSTKRTQDQRPQDVVSNYEKHLQNLKEEIAVLSAEKSGLQGRSARSPSPSTGTRSHRRGRSRSHSRSRSHSRSNSPCTTVAKIRSPSPNRAKMSSVARKAALLSRFSDAYSQARLDAQCLLRRCIDRAETVQRIIYIATVEAFHVAKMAFRHFKIRVRKMLTPSNVGSNTDFETAVSEYIVCHLDL-YDSQSSVNDVIRAMNVN-PKISFPPEVDFCLLTDFIQEICCIAFAMQSLEPPLDIAF-GADGEIFNDCKYRRSYDSDFTAPLVFYHVWPALMENDCVIMKGEAVT 487

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Mouse
Match: Spata18 (spermatogenesis associated 18 [Source:MGI Symbol;Acc:MGI:1920722])

HSP 1 Score: 191.045 bits (484), Expect = 6.663e-54
Identity = 143/470 (30.43%), Postives = 234/470 (49.79%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFST--GLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRNPHCLHSPICHSPCDPCHSHGCLL-------------DSCCTLHVDHCDPICH-------GKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLIGHS--HSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M+E L++L  + S   LQ ++ +W+++Y  N+CDQN+    E+IE  AKVQ QLF +L++ A EGG   G   IK RLLP+L   F S   G +++S +   +D     KN     R      D  ++D      E+ +     +        Q  + A  K  + +    LK+   S K   +          +Y+  +++LK+++A+L++ ++     S    SP     SH                 +S CT       P  +        +   L+++ +D +S +RLDA   L R  D  E  Q+II+ + +E+F+ AK AFR +K RVR  L   +   +   E +V  YI  H D+  D  S   +VIR+++   P I  P  + F ++  FI+E C IA+ M +L  P+D+    ++GE++++ +YRRSYDS+++A  + +HVWP L ++  VI KGEAVT
Sbjct:    1 MAESLKKLAKSESLQALQDKVTYWVNDYNSNSCDQNLNYCIELIEQVAKVQAQLFGILTVTAQEGGNNEGVETIKCRLLPLLQTSFSSVNMGKTAESEMCATQDFQLRSKN-----RDNSPDQDQHQSD-----NESFSETQPTQVQDELKSLQAQEDARHKTSENRRSEALKSDHRSTKRTQDQRPQDV--VSNYEKHLQNLKEEIAVLSAEKSGLQGRSARSPSPSTGTRSHRRGRSRSHSRSRSHSRSNSPCTTVAKIRSPSPNRAKMSSVARKAALLSRFSDAYSQARLDAQCLLRRCIDRAETVQRIIYIATVEAFHVAKMAFRHFKIRVRKMLTPSNVGSNTDFETAVSEYIVCHLDL-YDSQSSVNDVIRAMNVN-PKISFPPEVDFCLLTDFIQEICCIAFAMQSLEPPLDIAF-GADGEIFNDCKYRRSYDSDFTAPLVFYHVWPALMENDCVIMKGEAVT 455

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Mouse
Match: Spata18 (spermatogenesis associated 18 [Source:MGI Symbol;Acc:MGI:1920722])

HSP 1 Score: 129.798 bits (325), Expect = 3.226e-34
Identity = 74/196 (37.76%), Postives = 118/196 (60.20%), Query Frame = 3

Query:  816 GKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLIGHS--HSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
             +   L+++ +D +S +RLDA   L R  D  E  Q+II+ + +E+F+ AK AFR +K RVR  L   +   +   E +V  YI  H D+  D  S   +VIR+++   P I  P  + F ++  FI+E C IA+ M +L  P+D+    ++GE++++ +YRRSYDS+++A  + +HVWP L ++  VI KGEAVT
Sbjct:    5 ARKAALLSRFSDAYSQARLDAQCLLRRCIDRAETVQRIIYIATVEAFHVAKMAFRHFKIRVRKMLTPSNVGSNTDFETAVSEYIVCHLDL-YDSQSSVNDVIRAMNVN-PKISFPPEVDFCLLTDFIQEICCIAFAMQSLEPPLDIAF-GADGEIFNDCKYRRSYDSDFTAPLVFYHVWPALMENDCVIMKGEAVT 197

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Mouse
Match: Spata18 (spermatogenesis associated 18 [Source:MGI Symbol;Acc:MGI:1920722])

HSP 1 Score: 129.798 bits (325), Expect = 3.870e-32
Identity = 74/191 (38.74%), Postives = 117/191 (61.26%), Query Frame = 3

Query:  831 LVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLIGHS--HSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            L+++ +D +S +RLDA   L R  D  E  Q+II+ + +E+F+ AK AFR +K RVR  L   +   +   E +V  YI  H D+  D  S   +VIR+++   P I  P  + F ++  FI+E C IA+ M +L  P+D+    ++GE++++ +YRRSYDS+++A  + +HVWP L ++  VI KGEAVT
Sbjct:  186 LLSRFSDAYSQARLDAQCLLRRCIDRAETVQRIIYIATVEAFHVAKMAFRHFKIRVRKMLTPSNVGSNTDFETAVSEYIVCHLDL-YDSQSSVNDVIRAMNVN-PKISFPPEVDFCLLTDFIQEICCIAFAMQSLEPPLDIAF-GADGEIFNDCKYRRSYDSDFTAPLVFYHVWPALMENDCVIMKGEAVT 373

[Close]

BLAST of MIEAP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q503Q1|MIEAP_DANRE (Mitochondria-eating protein OS=Danio rerio OX=7955 GN=spata18 PE=2 SV=2)

HSP 1 Score: 277.33 bits (708), Expect = 1.053e-85
Identity = 166/449 (36.97%), Postives = 268/449 (59.69%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFF--STGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRNPHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLIG-HSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M++ LRRLVN+  +++LQ +L+ W  +Y   +CDQN+ R CE++EL +K+QGQLF +L+L A EGG Y G   +K+RLLP LG  F   ++ +SSD+SL +++++    + ++E+  ++E +L  +E  L  TR E +++K EL +  +EL+   ++SA+  +  + EI  LK++L   + + +L K K    D Y+ ++  L+D+V+ L + +           SP  P       +                 ++ +LV++ +DL++  RL++ + L +Y DD E  Q+I+F + +ESF AAK A+R +KSRVR  L   H    SLED+V  YI  + D+  DV +   +VI +++   P I  P  + F +I SFIREACRIA+ M  L  P+D+   +S+GELY + +YRRS+DSE++A  ++ HVWP L +   VI KGEAVT
Sbjct:    1 MADTLRRLVNSSPYSVLQDKLDAWYKDYHVYSCDQNLNRCCELVELTSKIQGQLFTILNLTAQEGGHYSGVDTLKSRLLPWLGSCFTIAASAVSSDTSLSLIQESVEKDRKIRELSSVHESNLQKIEDQLCSTRIELDSVKRELVDTHIELDSTKNKSATTLLATEDEILHLKSELRVAQDQLDLYKRKLDVLDDYERQVRILRDEVSFLNAEKTVLQDRLARSRSPS-PLLRRSRSVSPVRGESPTRAQLTSSSRHARLVSRFSDLYATERLESQSLLLKYIDDLETVQRILFIAAVESFQAAKLAYRQFKSRVRKTLSSTHIGPESLEDAVVDYIVRNLDL-YDVQTSINDVINAMNVN-PRISFPPEVDFVLISSFIREACRIAFAMQTLDPPLDLAF-SSDGELYSDVKYRRSFDSEFTAPLVAFHVWPALLEGDTVILKGEAVT 445

[Close]

BLAST of MIEAP domain-containing protein vs. UniProt/SwissProt
Match: sp|E1BW58|MIEAP_CHICK (Mitochondria-eating protein OS=Gallus gallus OX=9031 GN=SPATA18 PE=3 SV=1)

HSP 1 Score: 247.669 bits (631), Expect = 1.709e-74
Identity = 165/456 (36.18%), Postives = 255/456 (55.92%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGF--FSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKS-ENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRNPHCLHSPICHSPC-DPCHSHGCLLDSCCTLHVDHCDP-----ICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDL-IGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M+  L++L    S  ++Q +LE W  +Y+ N+CDQN+ + CE+IE+ + +QGQLF +L+  + E G Y G   IK RLLP LG  F   ++G   ++ L + +D+   ++ ++++       L DL+ +L  TR E N ++ +L + +L LE   +Q A+  +    EI  L+  L++ ++ E + L+   +  D  + +IE L+D+++IL + ++   L S I  S    P         S   L +  C P         ++  LV +  D++S  R DA   L  Y  D E  Q+II+ + +ESF+AAK A+R +K RVR  L IGHS   SLED+V  YI  H D+  DV +   EVIRS++   P I       F VI SFIRE CR+A+ M  L  P+DV     +GE + E +Y RS DS+Y+AA +++HVWP L ++ +VI KGEAVT
Sbjct:    1 MAGSLKKLAKAESCRLMQEKLESWSKDYEINSCDQNLNQCCELIEMTSVIQGQLFTILNETSRESGHYAGVDTIKTRLLPWLGTWFSHATSGRLFETGLFLNQDSTETERKLRQLATSQTLQLQDLQEELTSTRLELNHVQQDLAQTQLALEDTKTQLATTLLTAADEIIQLRAVLKASRAQEEDSLRRLDHLNDC-EQQIERLRDELSILDAQKS--VLQSRIARSRSPSPRRIRS---RSPSPLPLRSCSPGRARSTNASRHAFLVARFGDIYSKDRFDAERILRTYISDMEMVQRIIYTAAVESFHAAKMAYRQFKMRVRKTLSIGHSGPESLEDTVMDYIVRHEDL-YDVQASVNEVIRSMNIN-PKISSTPECDFAVISSFIRELCRVAFSMQTLTPPLDVAFGM-DGEFFSETKYHRSVDSDYTAALVAYHVWPALMENDVVIVKGEAVT 447

[Close]

BLAST of MIEAP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q498J5|MIEAP_XENLA (Mitochondria-eating protein OS=Xenopus laevis OX=8355 GN=spata18 PE=2 SV=1)

HSP 1 Score: 240.736 bits (613), Expect = 9.426e-72
Identity = 166/449 (36.97%), Postives = 258/449 (57.46%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGF--FSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRNPHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDL-IGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M++ LRRL N+    +LQ +L+ W  +Y  N+CD N+   CE++ELN+KVQGQLFK+LS+ A EGG Y G   IK+R LP LG  F   S G  S++S   L ++   K +       +E+ L+++E+ L+ TR E N+++ EL E +++LE   ++SA+  +  ++EI  L+ +L + + +  L    +   D Y+ +I  LKD+++IL+S  +          SP    HS         +            +  +L+++ ND+F+  RLDA T L RY  D +  Q+IIF + +ESF++AK AFR ++ RV   L   H    SLED+V  YI  + D+  DV S   EVI +++   P I  P  + F +I  FI+E CR+A+ M  L  P+D+   T +GEL+ + +YRR+YDSE++A  + +HVWP L ++  VI KGEAVT
Sbjct:    1 MADILRRLTNSERCRLLQDKLDDWYKDYHINSCDSNLNVCCELLELNSKVQGQLFKILSVTAQEGGQYAGVETIKSRFLPWLGTCFSTASPGSFSENSFVTLNESMERKLSTS-----HERELNEVESKLSSTRIELNSVRQELLETQMDLEDTKTKSANTLLATEEEILQLRAELRAAREKLELRSLDS--IDEYERQIRLLKDEISILSSESSILKSRLSRSRSPSPIRHSSRSSSPFARSESPTSAKLTSASRQARLISRFNDIFANDRLDAQTLLRRYIQDLDMVQRIIFIATVESFHSAKMAFRQFRLRVCKSLSPSHMGPESLEDAVIDYIVGNLDL-YDVQSSVNEVISAMNVN-PKISFPPEVDFILISGFIQEVCRVAFTMQTLDPPLDIAF-TVDGELFTDSKYRRTYDSEHTAPLVYYHVWPALMENDNVIVKGEAVT 439

[Close]

BLAST of MIEAP domain-containing protein vs. UniProt/SwissProt
Match: sp|E1BLK7|MIEAP_BOVIN (Mitochondria-eating protein OS=Bos taurus OX=9913 GN=SPATA18 PE=3 SV=1)

HSP 1 Score: 221.861 bits (564), Expect = 4.553e-64
Identity = 161/494 (32.59%), Postives = 254/494 (51.42%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFSTGLSS--DSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSEN-----NLLKTKAYKTDS--------------------------------YKHEIEHLKDDVAILASHRNPHCLHSPICHSPCD-PCHSHGCLLDSCCTLHVDHCDPICH-------GKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLIGH-SHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M++ LRRLV+  +   LQ +LE WL  Y  N+CDQN+    E+IE  AKVQGQLF +L++ A EGG Y G   IK+RLLP L   F +  L    DS +  L+DT    ++    K    + +  L+ DL+ TR + N ++ ++ E +  LE   ++SA   +  ++EI  LK QL+S++++      N  + ++ K  S                                Y+ ++  LKD++A+L++ ++     S    SP   PC        S          P  +        +   L+++ +D +S  RLDA   L R  D  E  Q+II+ + +E+F+ AK AFR +K RVR  L    + S   ED+V  YI  H D+  D  S   +VIR+++   P I  P  + F ++ +FI+E C IA+ M  L  P+D+    ++GE++++ +YRRSYDS+++A  + +HVWP L ++  VI KGEAVT
Sbjct:    1 MADNLRRLVSNEALRSLQDKLESWLREYNANSCDQNLNHCLELIEQVAKVQGQLFGILTIAAQEGGHYDGVETIKSRLLPWLEASFTAASLGKPVDSKVPSLQDTFDKDRH----KESGTRDIQQLDADLSATRNQLNQVQDDMAETEKTLEEPKNRSAISLLAAEEEINQLKKQLKSLQAQEESRHRNSDRRRSEKRGSERRRVELRGSEQRVSDLDRRSANQRNAEAVCDYEKQLRTLKDEIAVLSAEKSVLQGRSARSRSPSPAPCSRSHSRSRSTSPSSAKARTPSPNRAKLSSVARKAALLSRFSDAYSQGRLDAQCLLRRCIDKAETVQRIIYIATVEAFHVAKMAFRHFKIRVRKSLTPSCAGSNDFEDAVSDYIICHLDL-YDSQSSVNDVIRAMNVN-PKISFPPEVDFCLLSNFIQEICCIAFAMQTLEPPLDIAF-GADGEIFNDCKYRRSYDSDFTAPLVFYHVWPALMENDCVIMKGEAVT 487

[Close]

BLAST of MIEAP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8TC71|MIEAP_HUMAN (Mitochondria-eating protein OS=Homo sapiens OX=9606 GN=SPATA18 PE=1 SV=1)

HSP 1 Score: 213.772 bits (543), Expect = 4.509e-61
Identity = 163/493 (33.06%), Postives = 258/493 (52.33%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGF--FSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIK--------------SEN----------------NLLKTKAYKTDS-----YKHEIEHLKDDVAILASHRNP----HCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICH-----GKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLI-GHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M+E L+RLV+  +   LQ +L+ WL  Y  NTCDQN+    E+IE  AKVQGQLF +L+  A EGG   G   IK+RLLP L   F   S G S DS +  L+DT   +++     R  ++ +  L+++LN TR + N ++ +L E +  LE   ++SA   +  ++EI  LK QL+S++              SEN                N LK  A + D+     YK ++ +LK+++A+L++ ++              +P     S          + V    P         +   L+++ +D +S +RLDA   L R  D  E  Q+II+ + +E+F+ AK AFR +K  VR  L   +  S   E++V  Y+  H D+  D  S   +VIR+++   P I  P  + F ++  FI+E C IA+ M AL  P+D+ +  ++GE++++ +YRRSYDS+++A  + +HVWP L ++  VI KGEAVT
Sbjct:    1 MAENLKRLVSNETLRTLQEKLDFWLKEYNTNTCDQNLNHCLELIEQVAKVQGQLFGILTAAAQEGGRNDGVETIKSRLLPWLEASFTAASLGKSVDSKVPSLQDTFDRERHKDPSPR--DRDMQQLDSNLNSTRSQCNQVQDDLVETEKNLEESKNRSAISLLAAEEEINQLKKQLKSLQAQEDARHRNTDQRSSENRRSEPWSLEERKREQWNSLKQNADQQDTEAMSDYKKQLRNLKEEIAVLSAEKSALQGRSSRSRSPSPAPRSRSCSRSRSASPSTAVKVRRPSPNRSKLSNVARKAALLSRFSDSYSQARLDAQCLLRRCIDKAETVQRIIYIATVEAFHVAKMAFRHFKIHVRKSLTPSYVGSNDFENAVLDYVICHLDL-YDSQSSVNDVIRAMNVN-PKISFPPVVDFCLLSDFIQEICCIAFAMQALEPPLDIAY-GADGEVFNDCKYRRSYDSDFTAPLVLYHVWPALMENDCVIMKGEAVT 488

[Close]

BLAST of MIEAP domain-containing protein vs. TrEMBL
Match: A0A1S3K2R8 (mitochondria-eating protein isoform X1 OS=Lingula unguis OX=7574 GN=LOC106169214 PE=4 SV=1)

HSP 1 Score: 405.601 bits (1041), Expect = 3.156e-132
Identity = 220/461 (47.72%), Postives = 317/461 (68.76%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFSTG--LSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRNP---HCLHSPI---CHSPC------DPCHSHGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLIG-HSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M+E LRRLVN  SF+ILQ +LE WLD+Y  N+CDQNVAR CEIIELNAKVQGQ+FKLLSLCASEGG+YGGA  IK+RLLP LG+ FF++G  +++D+SL VL + AS  + + E++  YE+S+ DLE +L +T  EN+ L+ +L EA+ EL+R   +S S+KMF++ EIRDL+ +L   + EN  L+ K+   D Y+ ++  L++++AIL   RN    H  H P+    HS        D   ++  L      L  D  D     + Q L+T+ ND+F+  RLDAM  L RYSDDHENNQ+I+F +++E+F +AK AFR +K +VR++L   H    +LE++VQ YIN + D+  D+ SL  +V+R+L+  + +   PA +S+++IG+FIREAC++AW+MSAL  P+D+   +S+ EL+D+ +YRRSYDSEY+A  ++HH+W CL++   VI KGEA T
Sbjct:    1 MAESLRRLVNIASFSILQEKLERWLDDYHVNSCDQNVARCCEIIELNAKVQGQIFKLLSLCASEGGMYGGAGAIKSRLLPWLGQSFFASGGSVTADTSLSVLAEAASKDREINELQDQYERSIVDLEDNLRVTNIENDNLRTDLAEAQDELDRTKRESTSEKMFNESEIRDLRNKLALAEDENRNLREKSGLLDDYERQLRILREEIAILTGERNSVLKHRSHVPLYQHTHSSTQSIVNGDMSDTYRSLPRVSSPLSPD--DVTQRVRQQNLITRFNDMFAQDRLDAMDILRRYSDDHENNQRIVFAALVEAFASAKLAFRQFKLKVRSNLAATHVGPDTLEEAVQDYINRNTDL-YDLPSLQADVVRALNRNIKIFL-PADISYNIIGTFIREACKLAWQMSALAHPLDI-AVSSDAELFDDYKYRRSYDSEYTAPLVNHHIWACLQQGTKVIVKGEACT 456

[Close]

BLAST of MIEAP domain-containing protein vs. TrEMBL
Match: A0A1S3K339 (mitochondria-eating protein isoform X2 OS=Lingula unguis OX=7574 GN=LOC106169214 PE=4 SV=1)

HSP 1 Score: 404.831 bits (1039), Expect = 6.450e-132
Identity = 220/461 (47.72%), Postives = 317/461 (68.76%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFSTG--LSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRNP---HCLHSPI---CHSPC------DPCHSHGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLIG-HSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M+E LRRLVN  SF+ILQ +LE WLD+Y  N+CDQNVAR CEIIELNAKVQGQ+FKLLSLCASEGG+YGGA  IK+RLLP LG+ FF++G  +++D+SL VL + AS  + + E++  YE+S+ DLE +L +T  EN+ L+ +L EA+ EL+R   +S S+KMF++ EIRDL+ +L   + EN  L+ K+   D Y+ ++  L++++AIL   RN    H  H P+    HS        D   ++  L      L  D  D     + Q L+T+ ND+F+  RLDAM  L RYSDDHENNQ+I+F +++E+F +AK AFR +K +VR++L   H    +LE++VQ YIN + D+  D+ SL  +V+R+L+  + +   PA +S+++IG+FIREAC++AW+MSAL  P+D+   +S+ EL+D+ +YRRSYDSEY+A  ++HH+W CL++   VI KGEA T
Sbjct:    1 MAESLRRLVNIASFSILQEKLERWLDDYHVNSCDQNVARCCEIIELNAKVQGQIFKLLSLCASEGGMYGGAGAIKSRLLPWLGQSFFASGGSVTADTSLSVLAEAASKDREINELQDQYERSIVDLEDNLRVTNIENDNLRTDLAEAQDELDRTKRESTSEKMFNESEIRDLRNKLALAEDENRNLREKSGLLDDYERQLRILREEIAILTGERNSVLKHRSHVPLYQHTHSSTQSIVNGDMSDTYRSLPRVSSPLSPD--DVTQRVRQQNLITRFNDMFAQDRLDAMDILRRYSDDHENNQRIVFAALVEAFASAKLAFRQFKLKVRSNLAATHVGPDTLEEAVQDYINRNTDL-YDLPSLQADVVRALNRNIKIFL-PADISYNIIGTFIREACKLAWQMSALAHPLDI-AVSSDAELFDDYKYRRSYDSEYTAPLVNHHIWACLQQGTKVIVKGEACT 456

[Close]

BLAST of MIEAP domain-containing protein vs. TrEMBL
Match: A0A267GZM3 (MIEAP domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig011670g2 PE=4 SV=1)

HSP 1 Score: 399.823 bits (1026), Expect = 4.999e-130
Identity = 221/474 (46.62%), Postives = 313/474 (66.03%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYG-GASVIKNRLLPILGKGFFSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILAS-------------HRNPHCLHSPICHSPCDPCHSHGCLLDSCCT---LHVDHC--------DPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTD-LIGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSL--DFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M+E LRRLV+ GSF+ILQ +LE W  +Y+ NT D+NVA  CEI+ELNA+VQGQLFKLLSLCASEGG+YG GA+ I++RLLP+LG GFF++GLSSD+SL V+ D A+ ++ + +++R YE+SLD+LE +L   + EN  ++ ELE+ K EL+RQ   SAS+KMF + EIRDLK +L   ++EN+ L+ +    D Y+ ++ HL+++VAIL                  P     P   +P +   S G + +S  +    H  H         DP+   + Q LV + ND FS+SRL+AM +L RY DD  NNQKI++ +V+E+F+AAK AFR +KS+VR+  LI H    +LE++VQ YIN H D+  D++ + ++ IR L  ++++ L   P G+ +DV+  F+RE C +AW MSAL  P+D      EGEL+DE RYRRSYDSE+SA  +SHH+WP L +   V+ KGEA T
Sbjct:    1 MAESLRRLVDIGSFSILQEKLEKWSRDYRRNTSDENVATACEIVELNARVQGQLFKLLSLCASEGGLYGTGAAAIRSRLLPLLGHGFFASGLSSDASLSVIADAANRERELGDVRRSYEQSLDELEAELGKFKAENEDIRAELEDTKQELDRQSRASASEKMFSEAEIRDLKLKLSESEAENDRLRARVRVLDDYERQVRHLREEVAILQGRPPSLYPPPPLLPPPPPPPASRPRTPTPKEAPISDGEIAESVSSHLAYHGRHGPLSPLSPHDPVQRSRQQTLVARFNDAFSVSRLEAMETLRRYYDDQVNNQKIVYAAVLEAFSAAKLAFRAFKSKVRSQLLISHVGPDTLEEAVQDYINCHTDM-FDLHLMVQDAIRGLTRNYRLYL---PPGIGYDVLTPFLREMCHLAWGMSALAHPVDA-AVGYEGELFDEFRYRRSYDSEFSATLVSHHIWPALVQGTAVLVKGEACT 469

[Close]

BLAST of MIEAP domain-containing protein vs. TrEMBL
Match: A0A267DZK1 (MIEAP domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig011670g3 PE=4 SV=1)

HSP 1 Score: 397.512 bits (1020), Expect = 3.854e-129
Identity = 219/474 (46.20%), Postives = 312/474 (65.82%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYG-GASVIKNRLLPILGKGFFSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILAS-------------HRNPHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHC-----------DPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTD-LIGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSL--DFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M+E LRRLV+ GSF+ILQ +LE W  +Y+ NT D+NVA  CEI+ELNA+VQGQLFKLLSLCASEGG+YG GA+ I++RLLP+LG GFF++GLSSD+SL V+ D A+ ++ + +++R YE+SLD+LE +L   + EN  ++ ELE+ K EL+RQ   SAS+KMF + EIRDLK +L   ++EN+ L+ +    D Y+ ++ HL+++VAIL                  P     P   +P +   S G + +S  +    H            DP+   + Q LV + ND FS+SRL+AM +L RY DD  NNQKI++ +V+E+F+AAK AFR +KS+VR+  LI H    +LE++VQ YIN H D+  D++ + ++ IR L  ++++ L   P G+ +DV+  F+RE C +AW MSAL  P+D      +GEL+DE RYRRSYDSE+SA  +SHH+WP L +   V+ KGEA T
Sbjct:    1 MAESLRRLVDIGSFSILQEKLEKWSRDYRRNTSDENVATACEIVELNARVQGQLFKLLSLCASEGGLYGTGAAAIRSRLLPLLGHGFFASGLSSDASLSVIADAANRERELGDVRRSYEQSLDELEAELGKFKAENEDIRAELEDTKQELDRQSRASASEKMFSEAEIRDLKLKLSGSEAENDRLRARVRVLDDYERQVRHLREEVAILQGRPPSLYPPPPLLPPPPPPPASRPRTPTPKEAPISDGEIAESVSSHLAYHGRPGPLSPLSPHDPVQRSRQQTLVARFNDAFSVSRLEAMETLRRYYDDQVNNQKIVYAAVLEAFSAAKLAFRAFKSKVRSQLLISHVGPDTLEEAVQDYINCHTDM-FDLHLMVQDAIRGLTRNYRLYL---PPGIGYDVLTPFLREMCHLAWGMSALAHPVDA-AVGYDGELFDEFRYRRSYDSEFSATLVSHHIWPALVQGTAVLVKGEACT 469

[Close]

BLAST of MIEAP domain-containing protein vs. TrEMBL
Match: A0A267GXL8 (MIEAP domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig011670g1 PE=4 SV=1)

HSP 1 Score: 395.971 bits (1016), Expect = 1.425e-128
Identity = 218/474 (45.99%), Postives = 312/474 (65.82%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYG-GASVIKNRLLPILGKGFFSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILAS-------------HRNPHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHC-----------DPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTD-LIGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSL--DFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M+E LRRLV+ GSF+ILQ +LE W  +Y+ NT D+NVA  CEI+ELNA+VQGQLFKLLSLCASEGG+YG GA+ I++RLLP+LG GFF++GLSSD+SL V+ D A+ ++ + +++R YE+SLD+LE +L   + EN  ++ ELE+ K EL+RQ   SAS+KMF + EIRDLK +L   +++N+ L+ +    D Y+ ++ HL+++VAIL                  P     P   +P +   S G + +S  +    H            DP+   + Q LV + ND FS+SRL+AM +L RY DD  NNQKI++ +V+E+F+AAK AFR +KS+VR+  LI H    +LE++VQ YIN H D+  D++ + ++ IR L  ++++ L   P G+ +DV+  F+RE C +AW MSAL  P+D      +GEL+DE RYRRSYDSE+SA  +SHH+WP L +   V+ KGEA T
Sbjct:    1 MAESLRRLVDIGSFSILQEKLEKWSRDYRRNTSDENVATACEIVELNARVQGQLFKLLSLCASEGGLYGTGAAAIRSRLLPLLGHGFFASGLSSDASLSVIADAANRERELGDVRRSYEQSLDELEAELGKFKAENEDIRAELEDTKQELDRQSRASASEKMFSEAEIRDLKLKLSGSEADNDRLRARVRVLDDYERQVRHLREEVAILQGRPPSLYPPPPLLPPPPPPPASRPRTPTPKEAPISDGEIAESVSSHLAYHGRPGPLSPLSPHDPVQRSRQQTLVARFNDAFSVSRLEAMETLRRYYDDQVNNQKIVYAAVLEAFSAAKLAFRAFKSKVRSQLLISHMGPDTLEEAVQDYINCHTDM-FDLHLMVQDAIRGLTRNYRLYL---PPGIGYDVLTPFLREMCHLAWGMSALAHPVDA-AVGYDGELFDEFRYRRSYDSEFSATLVSHHIWPALVQGTAVLVKGEACT 469

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Cavefish
Match: spata18 (spermatogenesis associated 18 [Source:NCBI gene;Acc:103047636])

HSP 1 Score: 276.174 bits (705), Expect = 3.098e-86
Identity = 171/451 (37.92%), Postives = 265/451 (58.76%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFF--STGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRN--PHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDL-IGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M++ LRRLVNT S+++LQ +LE W  +Y   +CDQN+ R CE++EL +K+QGQLF +L+L A EGG Y GA  +K+RLLP L   F   S+ +S D+SL +++D    ++ ++E+   +E  +  +E  L  TR E +++K EL EAK++L+   ++S +  +  + EI  LK +L +   + ++ K K    + Y+ ++  L+D+V  L + +      L      SP  P  S         +            ++ +LV++ +DL++  RL+A + L RY DD E  Q+IIF + +ESF AAK A+R +K RVR  L   H    +LED+V  YI  + D+  DV +   +VI S++   P I  P  + F +I SFIRE CRIA+ M  L   +D+   +S+GELY + +YRRSYDSE++A  +++HVWP L +   VI KGEAVT
Sbjct:    1 MADTLRRLVNTSSYSVLQQKLEAWYKDYHVISCDQNLNRCCELVELTSKIQGQLFTILNLTAQEGGHYAGADTLKSRLLPWLSTCFTMASSSVSPDTSLNLIQDNVEKERKIRELSLSHESDMQKIEDQLCSTRIELSSVKQELTEAKVQLDSTKNKSVTTLLATEDEILHLKAELRAAYEQVDVYKRKLDVLEDYERQVRLLRDEVTFLTAEKTMLQERLVRSRSPSPILPRRSRTS--SPVRSESPTRAQLTNSSRHARLVSRFSDLYATERLEAQSFLRRYIDDLETVQRIIFIAAVESFQAAKLAYRQFKLRVRKTLSPTHVGPETLEDAVVDYIVRNLDL-YDVQTSVNDVINSMNVN-PRISFPPEVDFVLISSFIREMCRIAFAMQTLDPTLDLAF-SSDGELYSDMKYRRSYDSEFTAPLVAYHVWPALMEGDAVIVKGEAVT 446

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Cavefish
Match: spata18 (spermatogenesis associated 18 [Source:NCBI gene;Acc:103047636])

HSP 1 Score: 276.174 bits (705), Expect = 3.432e-86
Identity = 171/451 (37.92%), Postives = 265/451 (58.76%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFF--STGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRN--PHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDL-IGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M++ LRRLVNT S+++LQ +LE W  +Y   +CDQN+ R CE++EL +K+QGQLF +L+L A EGG Y GA  +K+RLLP L   F   S+ +S D+SL +++D    ++ ++E+   +E  +  +E  L  TR E +++K EL EAK++L+   ++S +  +  + EI  LK +L +   + ++ K K    + Y+ ++  L+D+V  L + +      L      SP  P  S         +            ++ +LV++ +DL++  RL+A + L RY DD E  Q+IIF + +ESF AAK A+R +K RVR  L   H    +LED+V  YI  + D+  DV +   +VI S++   P I  P  + F +I SFIRE CRIA+ M  L   +D+   +S+GELY + +YRRSYDSE++A  +++HVWP L +   VI KGEAVT
Sbjct:    1 MADTLRRLVNTSSYSVLQQKLEAWYKDYHVISCDQNLNRCCELVELTSKIQGQLFTILNLTAQEGGHYAGADTLKSRLLPWLSTCFTMASSSVSPDTSLNLIQDNVEKERKIRELSLSHESDMQKIEDQLCSTRIELSSVKQELTEAKVQLDSTKNKSVTTLLATEDEILHLKAELRAAYEQVDVYKRKLDVLEDYERQVRLLRDEVTFLTAEKTMLQERLVRSRSPSPILPRRSRTS--SPVRSESPTRAQLTNSSRHARLVSRFSDLYATERLEAQSFLRRYIDDLETVQRIIFIAAVESFQAAKLAYRQFKLRVRKTLSPTHVGPETLEDAVVDYIVRNLDL-YDVQTSVNDVINSMNVN-PRISFPPEVDFVLISSFIREMCRIAFAMQTLDPTLDLAF-SSDGELYSDMKYRRSYDSEFTAPLVAYHVWPALMEGDAVIVKGEAVT 446

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Sea Lamprey
Match: spata18 (spermatogenesis associated 18 [Source:NCBI gene;Acc:497395])

HSP 1 Score: 117.472 bits (293), Expect = 6.095e-29
Identity = 70/213 (32.86%), Postives = 127/213 (59.62%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGF-FSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHR 695
            M+E LRRL N+G++  LQ RL+ W D++  NTC++N++R CE+IE+ +KVQ QLF ++S  A+ GG   GA  IK+R LP L   F  S  +S D+SL ++ + A  ++ ++++   + + +  LE++L  T +  +  K  L+E   +LE   ++SA+  +  ++EI  L+  L++ + E  L   +  + DS + +  +L+ ++A L   +
Sbjct:    1 MAESLRRLANSGAYGALQDRLDSWQDDFHLNTCNENLSRCCELIEMTSKVQNQLFNIMSSTANRGGPTAGAETIKSRFLPWLSSSFTVSPCVSPDTSLTLIREAAEKERQLQDLSTRHMREVGSLESELQSTHQALSNAKHRLQETTYDLEDAKTKSATTLLASEEEILQLRADLQAAREEAEL--NRREREDSER-QARYLQAEMATLQQEK 210

HSP 2 Score: 57.7658 bits (138), Expect = 3.327e-9
Identity = 36/92 (39.13%), Postives = 54/92 (58.70%), Query Frame = 3

Query: 1017 HSHSCSLEDSVQHYINTHADIDV-DVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKR 1289
            H+   S+E++V  YI    ++D+ DV S   +VIR L    P I  P  + F ++  F+RE CR+A+EM AL   +DV     EGELY+E++
Sbjct:  318 HTGPESMEEAVLDYI---VNLDLYDVQSSVADVIRELHAD-PRISFPPEVEFALLAGFVRELCRVAFEMQALEPTLDVALAV-EGELYNERK 404

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010651.1 (pep scaffold:Pmarinus_7.0:GL488477:857:6764:1 gene:ENSPMAG00000009645.1 transcript:ENSPMAT00000010651.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.5362 bits (140), Expect = 2.544e-10
Identity = 49/178 (27.53%), Postives = 99/178 (55.62%), Query Frame = 3

Query:  150 NTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAIL 683
            N+ ++N++  CE+IE  +KVQ QL  ++S   ++GG    A   K+RLLP+      S  +S ++SL ++ +TA  ++ ++++   + + +  LE++L  TR+  +  K  L++   ELE   + SAS  +  ++EI  L+  L++ + E  +   +  + DS +    +L+ ++A L
Sbjct:    1 NSSNENLSHCCELIERTSKVQNQLLNMMSNKTNQGGPTASA---KSRLLPLQSSVAVSPCVSPNASLTLIRETAQKERQLQDLCARHVREVGSLESELRSTRQSLSNAKQRLKDMADELEASKANSASTYLASEEEILQLRADLQAAREEAEV--NRREREDSERQ-ARYLQAEMATL 172

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Nematostella
Match: EDO49651 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFS0])

HSP 1 Score: 159.073 bits (401), Expect = 8.220e-43
Identity = 130/454 (28.63%), Postives = 211/454 (46.48%), Query Frame = 3

Query:   72 LRRLVNTGSFNILQSRLEHWLD-NYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNL-LKTKAYKTDSYKHEIEHLKDDVAILASHRNPHCL---HSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVR-------TDLIGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            L  ++ +    +L  RL    D +Y+Y T D++    CEIIE N ++Q +LF+LL LC  EGG YGG   +KN+L+P+L  G   + +     +K++ D              YE  +  LE DL   +RE             E  +   +   D  F  +++ +L+ +L    S+ NL L+    +    + E   LK + + L +      L    S        P +++G L             P+ H +  +LV +  +L+   RL+ M +L R   D   N+++ F  V E F   K+  R  + +VR         LI  S +  + DS   Y++ + + + DV +L  E+++ L      + +       +I  FIR   R+ W M AL  P+D+     +G++ +E RYRRSY+SE+SA  + H VWP L +DG V+AKGE VT
Sbjct:    9 LSNIIASADLKVLHERLITLFDESYRYTT-DESTTVCCEIIEENLRIQAKLFRLLQLCCDEGGTYGGFQPLKNKLMPLLCNGLVMSHIGGSEDVKLVRDK-------------YEHMVSRLEDDLKSAQREVG-ELRLKLSENEEDVKCIRRQLKDSYFADRQVTELQKRL----SDANLELEASELEIRKVREETGQLKKENSDLHARLQKQMLVENESEPVRMITKP-YANGIL-------------PL-HARAPRLVERFAELYGGERLELMDAL-RPMGDRAENERLSFCIVEECFMVCKTEQRRMRKKVRHALQPPNGSLIDASQTAEIVDS---YMSRNTE-NFDVETLIPEILKCLHRNAS-VNQSIPYKEKLILPFIRACVRVVWSMVALQPPIDIAIAL-DGDIINEARYRRSYNSEFSATVVEHFVWPALMRDGKVVAKGEVVT 421

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Medaka
Match: spata18 (spermatogenesis associated 18 [Source:NCBI gene;Acc:101174412])

HSP 1 Score: 262.307 bits (669), Expect = 7.536e-81
Identity = 170/463 (36.72%), Postives = 261/463 (56.37%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFSTG--LSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKD-------DVAILASH--RNPHCLHSPICHSPCDPCHS----HGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDL-IGHSHSCSLEDSVQHYINTHADI-DVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M+E LRRL  T SF +LQ +LE W  +Y+  +CDQN+ R CE+IEL AK+Q QLF +L+L A+EGG Y G   +K RLLP LG  F   G  +S+D+SL++++D+A   + ++++   +   +  +ET L+ TR +  +++ +L   + ELE   S+SA+  +  ++EI  LK  L S   +  + K +    D Y+ +I  L++       + A+L     R+P  L  P    P  P  S       L DS               +   LV++ + L+++ RL+A   L RY  D E  QKIIF +V+ESF +AK A+R +K RV+T L   H    SLED+   YI  + D+ DV  N    +VI +++   P I  P  + F +I + IRE CR+A+ M  L  P+D+    S+GEL+++ +YRRS+DS+++A  ++ HVWP L +   VI KGEAVT
Sbjct:    1 MAESLRRLTQTASFRVLQDKLESWHRDYRTISCDQNLNRCCELIELTAKLQSQLFAILNLAAAEGGHYAGVDTLKTRLLPWLGTSFSIVGPSVSADTSLQLMQDSAEKDRKIRDLSLSHGSDMQKMETQLSSTRLQLESVRAQLLHVQKELEETKSRSATTLLATEEEILQLKADLHSAYEQVEIYKRRIRAPDDYERQIRLLREELLHLNMEKAMLQDRLVRSPSPL--PRLSRPSSPLRSESPTRAQLSDSS--------------RRAGLVSRFSGLYAVERLEAQNLLRRYITDLEMVQKIIFIAVVESFRSAKLAYRQFKLRVKTTLSSSHFGPESLEDAAVDYIVRNLDLYDVQNN----DVINAMNVN-PQISFPPEVDFSLISALIRETCRLAFAMQTLDPPLDLAF-ASDGELFNDTKYRRSFDSDFTAPLVTFHVWPALMEGNGVIVKGEAVT 441

[Close]

BLAST of MIEAP domain-containing protein vs. Ensembl Medaka
Match: spata18 (spermatogenesis associated 18 [Source:NCBI gene;Acc:101174412])

HSP 1 Score: 261.922 bits (668), Expect = 1.682e-80
Identity = 170/463 (36.72%), Postives = 261/463 (56.37%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFSTG--LSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKD-------DVAILASH--RNPHCLHSPICHSPCDPCHS----HGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDL-IGHSHSCSLEDSVQHYINTHADI-DVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            M+E LRRL  T SF +LQ +LE W  +Y+  +CDQN+ R CE+IEL AK+Q QLF +L+L A+EGG Y G   +K RLLP LG  F   G  +S+D+SL++++D+A   + ++++   +   +  +ET L+ TR +  +++ +L   + ELE   S+SA+  +  ++EI  LK  L S   +  + K +    D Y+ +I  L++       + A+L     R+P  L  P    P  P  S       L DS               +   LV++ + L+++ RL+A   L RY  D E  QKIIF +V+ESF +AK A+R +K RV+T L   H    SLED+   YI  + D+ DV  N    +VI +++   P I  P  + F +I + IRE CR+A+ M  L  P+D+    S+GEL+++ +YRRS+DS+++A  ++ HVWP L +   VI KGEAVT
Sbjct:    1 MAESLRRLTQTASFRVLQDKLESWHRDYRTISCDQNLNRCCELIELTAKLQSQLFAILNLAAAEGGHYAGVDTLKTRLLPWLGTSFSIVGPSVSADTSLQLMQDSAEKDRKIRDLSLSHGSDMQKMETQLSSTRLQLESVRAQLLHVQKELEETKSRSATTLLATEEEILQLKADLHSAYEQVEIYKRRIRAPDDYERQIRLLREELLHLNMEKAMLQDRLVRSPSPL--PRLSRPSSPLRSESPTRAQLSDS--------------SRRAGLVSRFSGLYAVERLEAQNLLRRYITDLEMVQKIIFIAVVESFRSAKLAYRQFKLRVKTTLSSSHFGPESLEDAAVDYIVRNLDLYDVQNN----DVINAMNVN-PQISFPPEVDFSLISALIRETCRLAFAMQTLDPPLDLAF-ASDGELFNDTKYRRSFDSDFTAPLVTFHVWPALMEGNGVIVKGEAVT 441

[Close]

BLAST of MIEAP domain-containing protein vs. Planmine SMEST
Match: SMESG000019081.1 (SMESG000019081.1)

HSP 1 Score: 895.575 bits (2313), Expect = 0.000e+0
Identity = 445/446 (99.78%), Postives = 445/446 (99.78%), Query Frame = 3

Query:   60 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFSTGLSSDSSLKVLEDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTXXXXXXXXXXXXXRQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRNPHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLIGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFSTGLSSDSSLKVLED ASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTLKLELEEAKLELERQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRNPHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLIGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT
Sbjct:    1 MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFSTGLSSDSSLKVLEDAASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTLKLELEEAKLELERQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEHLKDDVAILASHRNPHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPICHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRTDLIGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 446

[Close]

BLAST of MIEAP domain-containing protein vs. Planmine SMEST
Match: SMESG000057251.1 (SMESG000057251.1)

HSP 1 Score: 117.472 bits (293), Expect = 3.556e-28
Identity = 71/193 (36.79%), Postives = 106/193 (54.92%), Query Frame = 3

Query:  825 QQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFNAAKSAFRLYKSRVRT--DLIGHSHSCSLEDSVQHYINTHADIDVDVNSLSREVIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPTSEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVT 1397
            Q LV + NDLFS SR+ AM  L  Y+DDHENNQ+I+F  VM S        R +++R+R   +LI H    ++  SV   I T    D+  N  S        F++P       LSF  +   I    ++ W        +++ +  ++GE++DE +YRRSYDS+++A  +SHH+WPCL ++  V+ KGEA T
Sbjct:  213 QGLVGRYNDLFSESRITAMDHLRLYNDDHENNQRILFALVMISAKCVSEFQRCHRNRIRNKNELI-HQFRETISRSVLKNIRTQIIYDLINNRGS-----YYRFQIP-----QNLSFVELEGLINNTIQLFWNFGGAQYNLELLN-AADGEMFDEHKYRRSYDSDFTAPLVSHHIWPCLIQNHRVLVKGEACT 393

HSP 2 Score: 99.3673 bits (246), Expect = 4.104e-22
Identity = 45/106 (42.45%), Postives = 66/106 (62.26%), Query Frame = 3

Query:   66 EPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKVQGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFSTGLSSDSSLKVLEDTASNKK 383
            E L    NT  F ILQ +L +W   +K+N  + N+++ CE+IELNA++Q QLFKLL  C   GG+ G    +KN+L+P LGK +F  GL+S+  + +L D   + K
Sbjct:    2 ESLNSFANTNCFYILQEKLNNWKHEFKHNCTEANISKCCELIELNARIQTQLFKLLGFCGIYGGLEGSEIKLKNQLIPYLGKDYFDNGLTSNVVMNILNDVKKHTK 107

The following BLAST results are available for this feature:

BLAST of MIEAP domain-containing protein vs. Ensembl Human
BLAST of MIEAP domain-containing protein vs. Ensembl Celegans
BLAST of MIEAP domain-containing protein vs. Ensembl Fly
BLAST of MIEAP domain-containing protein vs. Ensembl Zebrafish
BLAST of MIEAP domain-containing protein vs. Ensembl Xenopus
BLAST of MIEAP domain-containing protein vs. Ensembl Mouse
BLAST of MIEAP domain-containing protein vs. UniProt/SwissProt
BLAST of MIEAP domain-containing protein vs. TrEMBL
BLAST of MIEAP domain-containing protein vs. Ensembl Cavefish
BLAST of MIEAP domain-containing protein vs. Ensembl Sea Lamprey
BLAST of MIEAP domain-containing protein vs. Ensembl Yeast
BLAST of MIEAP domain-containing protein vs. Ensembl Nematostella
BLAST of MIEAP domain-containing protein vs. Ensembl Medaka
BLAST of MIEAP domain-containing protein vs. Planmine SMEST

BLAST of MIEAP domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4


Match Name	E-value	Identity	Description
SPATA18	4.327e-62	33.06	spermatogenesis associated 18 [Source:HGNC Symbol;...	[more]
SPATA18	9.388e-62	33.06	spermatogenesis associated 18 [Source:HGNC Symbol;...	[more]
SPATA18	3.545e-61	33.40	spermatogenesis associated 18 [Source:HGNC Symbol;...	[more]
SPATA18	4.906e-20	47.57	spermatogenesis associated 18 [Source:HGNC Symbol;...	[more]

BLAST of MIEAP domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of MIEAP domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of MIEAP domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
spata18	1.395e-86	36.97	spermatogenesis associated 18 [Source:NCBI gene;Ac...	[more]

BLAST of MIEAP domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4


Match Name	E-value	Identity	Description
ENSXETT00000051448.1	1.044e-79	37.33	spermatogenesis associated 18 [Source:NCBI gene;Ac...	[more]
itga8	1.988e-75	36.53	integrin subunit alpha 8 [Source:Xenbase;Acc:XB-GE...	[more]
ENSXETT00000051452.1	1.578e-74	36.06	spermatogenesis associated 18 [Source:NCBI gene;Ac...	[more]
itga8	2.695e-74	35.36	integrin subunit alpha 8 [Source:Xenbase;Acc:XB-GE...	[more]

BLAST of MIEAP domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4


Match Name	E-value	Identity	Description
Spata18	1.594e-55	30.24	spermatogenesis associated 18 [Source:MGI Symbol;A...	[more]
Spata18	6.663e-54	30.43	spermatogenesis associated 18 [Source:MGI Symbol;A...	[more]
Spata18	3.226e-34	37.76	spermatogenesis associated 18 [Source:MGI Symbol;A...	[more]
Spata18	3.870e-32	38.74	spermatogenesis associated 18 [Source:MGI Symbol;A...	[more]

BLAST of MIEAP domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q503Q1\|MIEAP_DANRE	1.053e-85	36.97	Mitochondria-eating protein OS=Danio rerio OX=7955...	[more]
sp\|E1BW58\|MIEAP_CHICK	1.709e-74	36.18	Mitochondria-eating protein OS=Gallus gallus OX=90...	[more]
sp\|Q498J5\|MIEAP_XENLA	9.426e-72	36.97	Mitochondria-eating protein OS=Xenopus laevis OX=8...	[more]
sp\|E1BLK7\|MIEAP_BOVIN	4.553e-64	32.59	Mitochondria-eating protein OS=Bos taurus OX=9913 ...	[more]
sp\|Q8TC71\|MIEAP_HUMAN	4.509e-61	33.06	Mitochondria-eating protein OS=Homo sapiens OX=960...	[more]

BLAST of MIEAP domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1S3K2R8	3.156e-132	47.72	mitochondria-eating protein isoform X1 OS=Lingula ...	[more]
A0A1S3K339	6.450e-132	47.72	mitochondria-eating protein isoform X2 OS=Lingula ...	[more]
A0A267GZM3	4.999e-130	46.62	MIEAP domain-containing protein OS=Macrostomum lig...	[more]
A0A267DZK1	3.854e-129	46.20	MIEAP domain-containing protein OS=Macrostomum lig...	[more]
A0A267GXL8	1.425e-128	45.99	MIEAP domain-containing protein OS=Macrostomum lig...	[more]

BLAST of MIEAP domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
spata18	3.098e-86	37.92	spermatogenesis associated 18 [Source:NCBI gene;Ac...	[more]
spata18	3.432e-86	37.92	spermatogenesis associated 18 [Source:NCBI gene;Ac...	[more]

BLAST of MIEAP domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2


Match Name	E-value	Identity	Description
spata18	6.095e-29	32.86	spermatogenesis associated 18 [Source:NCBI gene;Ac...	[more]
ENSPMAT00000010651.1	2.544e-10	27.53	pep scaffold:Pmarinus_7.0:GL488477:857:6764:1 gene...	[more]

BLAST of MIEAP domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of MIEAP domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO49651	8.220e-43	28.63	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of MIEAP domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
spata18	7.536e-81	36.72	spermatogenesis associated 18 [Source:NCBI gene;Ac...	[more]
spata18	1.682e-80	36.72	spermatogenesis associated 18 [Source:NCBI gene;Ac...	[more]

BLAST of MIEAP domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000019081.1	0.000e+0	99.78	SMESG000019081.1	[more]
SMESG000057251.1	3.556e-28	36.79	SMESG000057251.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30013149 ID=SMED30013149|Name=MIEAP domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1668bp
AATTACCTTAGTAATTAAACCAAGTTCATTTCAAATTATTTAACAACAAC
TGTTTAGATATGAGTGAACCTCTTAGAAGACTGGTAAATACTGGCTCATT
TAATATTCTTCAAAGTAGACTAGAGCATTGGTTAGATAATTATAAGTATA
ATACATGTGATCAGAATGTTGCCCGCGGCTGTGAAATAATTGAATTAAAT
GCAAAGGTTCAAGGACAACTCTTTAAACTGTTGAGTCTATGTGCTTCTGA
AGGTGGAGTTTATGGAGGGGCAAGCGTGATTAAAAATCGGTTGCTTCCAA
TTTTGGGCAAAGGATTTTTCTCTACTGGATTGAGTTCTGACAGCAGTTTA
AAGGTATTGGAAGACACAGCATCAAATAAAAAAAATGTTAAGGAAATTAA
AAGAATTTACGAGAAATCTCTCGATGATTTAGAAACTGATTTAAATTTAA
CAAGACGAGAAAATAACACATTAAAACTCGAATTGGAAGAGGCAAAGCTG
GAATTGGAGCGTCAAGGCAGCCAATCAGCAAGCGATAAAATGTTTGATAA
GCAAGAAATTAGAGATTTAAAAACTCAATTGGAAAGCATTAAATCAGAAA
ACAATCTATTGAAAACAAAAGCTTACAAGACAGACTCATACAAGCATGAA
ATTGAACACTTAAAAGATGACGTTGCAATCCTTGCATCCCATAGAAATCC
CCATTGTTTGCATTCACCAATATGCCATTCTCCATGTGATCCCTGCCATT
CTCATGGTTGTTTGTTAGATTCGTGTTGCACTCTTCACGTAGATCATTGT
GATCCGATTTGCCATGGAAAGTACCAACAACTAGTCACAAAAATTAATGA
TCTATTTTCAATTTCCCGACTTGATGCCATGACTTCTCTCAGCCGATATA
GTGATGACCATGAAAATAACCAGAAAATTATATTCCATTCTGTCATGGAA
TCATTCAATGCCGCAAAATCTGCTTTCAGATTATACAAATCTCGAGTGAG
AACTGACCTAATTGGTCATTCACATAGTTGCAGTTTGGAAGACTCGGTGC
AACATTATATTAATACTCATGCTGACATTGATGTCGATGTGAACAGCTTG
AGTCGGGAAGTTATAAGATCTTTAGATTTCAAAGTTCCTTTAATTAAAAG
ACCAGCTGGATTAAGTTTTGATGTTATTGGTTCATTTATTAGAGAAGCAT
GCCGAATTGCATGGGAAATGTCCGCTTTACCTATACCTATGGACGTTCAC
CACCCTACCTCCGAAGGTGAATTGTATGATGAAAAAAGATATCGAAGAAG
TTATGATTCTGAATACAGTGCTGCTTTTATTTCACATCATGTTTGGCCTT
GTCTCGAAAAGGATGGAATCGTAATTGCTAAAGGCGAGGCTGTTACAAAA
CGATCTGGATCTCTAACAAAGTCACTGAGATCTAGTCGGAGTCCAACAAG
ATCACCGACGAGAATTAGTGGAAGATCTCCTTCTCCAATTTTCCAAAAAA
GTAGATCTCCAACGAGTTTTCGATCAACAAGTCCAACTGCCAGAAGGTCA
AAACTGTTTTGAAATGGGTTTTCCTAATAGACTTTGCTAACTTTTCAAAA
CAATTTTAGACTTTAATGTTCAGCATATGAAATAATTATTATTTGCGAAT
CCAATCCATATTATTAGC

protein sequence of SMED30013149-orf-1

>SMED30013149-orf-1 ID=SMED30013149-orf-1|Name=SMED30013149-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=501bp
MSEPLRRLVNTGSFNILQSRLEHWLDNYKYNTCDQNVARGCEIIELNAKV
QGQLFKLLSLCASEGGVYGGASVIKNRLLPILGKGFFSTGLSSDSSLKVL
EDTASNKKNVKEIKRIYEKSLDDLETDLNLTRRENNTLKLELEEAKLELE
RQGSQSASDKMFDKQEIRDLKTQLESIKSENNLLKTKAYKTDSYKHEIEH
LKDDVAILASHRNPHCLHSPICHSPCDPCHSHGCLLDSCCTLHVDHCDPI
CHGKYQQLVTKINDLFSISRLDAMTSLSRYSDDHENNQKIIFHSVMESFN
AAKSAFRLYKSRVRTDLIGHSHSCSLEDSVQHYINTHADIDVDVNSLSRE
VIRSLDFKVPLIKRPAGLSFDVIGSFIREACRIAWEMSALPIPMDVHHPT
SEGELYDEKRYRRSYDSEYSAAFISHHVWPCLEKDGIVIAKGEAVTKRSG
SLTKSLRSSRSPTRSPTRISGRSPSPIFQKSRSPTSFRSTSPTARRSKLF
*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **biological process**
Term	Definition
GO:0035694	mitochondrial protein catabolic process
GO:0033554	cellular response to stress

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000034	epidermis
PLANA:0000096	ventral epidermis
PLANA:0000106	ciliated epithelial cell
PLANA:0000418	head
PLANA:0000420	parapharyngeal region

Vocabulary: **INTERPRO**
Term	Definition
IPR026169	MIEAP
IPR031981	MIEAP_C

Vocabulary: **cellular component**
Term	Definition
GO:0005741	mitochondrial outer membrane

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 107..155
None	No IPR available	COILS	Coil	Coil	coord: 160..187
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 450..500
None	No IPR available	PANTHER	PTHR21771:SF0	MITOCHONDRIA-EATING PROTEIN	coord: 1..490
IPR031981	Mitochondria-eating protein, C-terminal domain	PFAM	PF16026	MIEAP	coord: 256..444 e-value: 2.8E-37 score: 128.9
IPR026169	Mitochondria-eating protein	PANTHER	PTHR21771	FAMILY NOT NAMED	coord: 1..490

Main menu

MIEAP domain-containing protein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 10

Owlery

blazegraph-runner