SMED30013087
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30013087 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Alignments
SMED30013087 aligns in the following genomic locations:
Homology
BLAST of SMED30013087 vs. Ensembl Human
Match: MYCBPAP (MYCBP associated protein [Source:HGNC Symbol;Acc:HGNC:19677]) HSP 1 Score: 63.5438 bits (153), Expect = 1.448e-9 Identity = 47/188 (25.00%), Postives = 85/188 (45.21%), Query Frame = 3 Query: 1266 HIHFPATIMGVNEIKNLYFANRGSCTINFSWKKIEIERHLKTDDFH-------SAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLSSKKII-FHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKEL 1805 H+ F T+ G L N G+ I + W+ R + D F F+F +G++LP F+S G F E WE T P L I+ + HA+SL D F ER ++E + + + +E++ +L+ + + +D + L +E++F++ NP + + ++V+ L Sbjct: 427 HLTF-ETLEGEKTSSELTVVNNGTVAIWYDWR-----RQHQPDTFQDLKKNRMQRFYFDNREGVILPGEIKTFTFFFKSLTAGVFREFWEFRTHPTLLGGAILQVNLHAVSLTQDVFEDERKVLESKLTAHEAVTVVREVLQELLMGVLTPERTPSPVD---AYLTEEDLFRHRNPPLHYEHQVVQSL 605
BLAST of SMED30013087 vs. Ensembl Human
Match: MYCBPAP (MYCBP associated protein [Source:HGNC Symbol;Acc:HGNC:19677]) HSP 1 Score: 63.5438 bits (153), Expect = 1.477e-9 Identity = 47/188 (25.00%), Postives = 85/188 (45.21%), Query Frame = 3 Query: 1266 HIHFPATIMGVNEIKNLYFANRGSCTINFSWKKIEIERHLKTDDFH-------SAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLSSKKII-FHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKEL 1805 H+ F T+ G L N G+ I + W+ R + D F F+F +G++LP F+S G F E WE T P L I+ + HA+SL D F ER ++E + + + +E++ +L+ + + +D + L +E++F++ NP + + ++V+ L Sbjct: 433 HLTF-ETLEGEKTSSELTVVNNGTVAIWYDWR-----RQHQPDTFQDLKKNRMQRFYFDNREGVILPGEIKTFTFFFKSLTAGVFREFWEFRTHPTLLGGAILQVNLHAVSLTQDVFEDERKVLESKLTAHEAVTVVREVLQELLMGVLTPERTPSPVD---AYLTEEDLFRHRNPPLHYEHQVVQSL 611
BLAST of SMED30013087 vs. Ensembl Human
Match: MYCBPAP (MYCBP associated protein [Source:HGNC Symbol;Acc:HGNC:19677]) HSP 1 Score: 63.5438 bits (153), Expect = 1.514e-9 Identity = 47/188 (25.00%), Postives = 85/188 (45.21%), Query Frame = 3 Query: 1266 HIHFPATIMGVNEIKNLYFANRGSCTINFSWKKIEIERHLKTDDFH-------SAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLSSKKII-FHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKEL 1805 H+ F T+ G L N G+ I + W+ R + D F F+F +G++LP F+S G F E WE T P L I+ + HA+SL D F ER ++E + + + +E++ +L+ + + +D + L +E++F++ NP + + ++V+ L Sbjct: 427 HLTF-ETLEGEKTSSELTVVNNGTVAIWYDWR-----RQHQPDTFQDLKKNRMQRFYFDNREGVILPGEIKTFTFFFKSLTAGVFREFWEFRTHPTLLGGAILQVNLHAVSLTQDVFEDERKVLESKLTAHEAVTVVREVLQELLMGVLTPERTPSPVD---AYLTEEDLFRHRNPPLHYEHQVVQSL 605
BLAST of SMED30013087 vs. Ensembl Human
Match: MYCBPAP (MYCBP associated protein [Source:HGNC Symbol;Acc:HGNC:19677]) HSP 1 Score: 63.5438 bits (153), Expect = 1.565e-9 Identity = 47/188 (25.00%), Postives = 85/188 (45.21%), Query Frame = 3 Query: 1266 HIHFPATIMGVNEIKNLYFANRGSCTINFSWKKIEIERHLKTDDFH-------SAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLSSKKII-FHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKEL 1805 H+ F T+ G L N G+ I + W+ R + D F F+F +G++LP F+S G F E WE T P L I+ + HA+SL D F ER ++E + + + +E++ +L+ + + +D + L +E++F++ NP + + ++V+ L Sbjct: 470 HLTF-ETLEGEKTSSELTVVNNGTVAIWYDWR-----RQHQPDTFQDLKKNRMQRFYFDNREGVILPGEIKTFTFFFKSLTAGVFREFWEFRTHPTLLGGAILQVNLHAVSLTQDVFEDERKVLESKLTAHEAVTVVREVLQELLMGVLTPERTPSPVD---AYLTEEDLFRHRNPPLHYEHQVVQSL 648
BLAST of SMED30013087 vs. Ensembl Zebrafish
Match: mycbpap (mycbp associated protein [Source:ZFIN;Acc:ZDB-GENE-080923-1]) HSP 1 Score: 60.4622 bits (145), Expect = 7.836e-9 Identity = 56/203 (27.59%), Postives = 92/203 (45.32%), Query Frame = 3 Query: 1284 TIMGVNEIKNLYFANRGSCTINFSWKKIEIERHLKTDDFHSAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVL-SSKKIIFHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRY--AVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKELK-LFA--LQNFEIDWDLSLDSLKQML 1874 T+ G + +L N GS I +SW+K+ T F+F ++LP S I VTF+S G E+W L T PVL + ++L D +R +++ + Q + +I DL+ + R + E+ + DEE F NP + + V+ L+ L+A ++ E WDLS+ +L Q+L Sbjct: 422 TVTGQSVNADLELKNEGSTVIYYSWEKL-------TPTHTQHFYFNNTTAVILPSSTKHICVTFKSASAGILTEVWRLNTHPVLMGGASVHVILRGVALDQDKSAEQRMALQKELEQKACVCFCSRLILDLLTGVESPERPRSPDEL-----YITDEERFHTHNPNLQFQRGPVEALQSLYAEVKRSEEQRWDLSIHTLTQVL 612
BLAST of SMED30013087 vs. Ensembl Xenopus
Match: kif11 (kinesin family member 11 [Source:Xenbase;Acc:XB-GENE-978999]) HSP 1 Score: 63.1586 bits (152), Expect = 1.688e-9 Identity = 44/183 (24.04%), Postives = 82/183 (44.81%), Query Frame = 3 Query: 1314 LYFANRGSCTINFSWKKI----EIERHLKTDDFHSAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLSSKKII-FHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKELKLFALQNFEIDWDL 1847 L N GS I + W+++ ++R K F+F + G++LP I P F+S G F E WE T PVL + ++ I+L D R ++ + + +++ I+++L+ + + + + +EE F +NP++++ + V+ELK WDL Sbjct: 427 LEVVNNGSAAIWYEWRRLPPPARLQRSRKEPRVQR-FYFNTSSGVILPGETRIFPFHFKSPTAGIFGENWEFCTHPVLLAGALLEVSLWGIALHEDKTAPLREALQRELEAREALVVAQHIVEELLEGVRSPER----PQSPVRSVTEEEAFSTVNPKLYYKHQTVQELKHL--------WDL 596
BLAST of SMED30013087 vs. Ensembl Xenopus
Match: kif11 (kinesin family member 11 [Source:Xenbase;Acc:XB-GENE-978999]) HSP 1 Score: 61.6178 bits (148), Expect = 5.327e-9 Identity = 44/183 (24.04%), Postives = 82/183 (44.81%), Query Frame = 3 Query: 1314 LYFANRGSCTINFSWKKI----EIERHLKTDDFHSAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLSSKKII-FHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKELKLFALQNFEIDWDL 1847 L N GS I + W+++ ++R K F+F + G++LP I P F+S G F E WE T PVL + ++ I+L D R ++ + + +++ I+++L+ + + + + +EE F +NP++++ + V+ELK WDL Sbjct: 428 LEVVNNGSAAIWYEWRRLPPPARLQRSRKEPRVQR-FYFNTSSGVILPGETRIFPFHFKSPTAGIFGENWEFCTHPVLLAGALLEVSLWGIALHEDKTAPLREALQVELEAREALVVAQHIVEELLEGVRSPER----PQSPVRSVTEEEAFSTVNPKLYYKHQTVQELKHL--------WDL 597
BLAST of SMED30013087 vs. Ensembl Mouse
Match: Mycbpap (MYCBP associated protein [Source:MGI Symbol;Acc:MGI:2388726]) HSP 1 Score: 65.0846 bits (157), Expect = 3.258e-10 Identity = 48/178 (26.97%), Postives = 83/178 (46.63%), Query Frame = 3 Query: 1284 TIMGVNEIKNLYFANRGSCTINFSWKKIEIE---RHLKTDDFHSAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLSSKKII-FHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKEL 1805 T+ G L N G+ I ++W++ + + LK + F+F +G++LP F+S G F E WE T P L ++ HAISL D F+ ER L+E + I I++ ++ DL+R + +D + L +E++F NP + + ++V+ L Sbjct: 432 TLEGERTSSELTVVNNGTVAIWYNWRRRPHQDFFQDLKQNKTQR-FYFNNREGVILPGETKHFTFFFKSLNAGIFRESWEFGTHPTLLGGAVLQVTLHAISLTQDIFMDERKLLETKLAAHEAITIAQSVLQDLLRGISTPERTPSPVD---AYLTEEDLFNYRNPRLHYQHQVVQNL 605
BLAST of SMED30013087 vs. Ensembl Mouse
Match: Mycbpap (MYCBP associated protein [Source:MGI Symbol;Acc:MGI:2388726]) HSP 1 Score: 65.0846 bits (157), Expect = 3.329e-10 Identity = 48/178 (26.97%), Postives = 83/178 (46.63%), Query Frame = 3 Query: 1284 TIMGVNEIKNLYFANRGSCTINFSWKKIEIE---RHLKTDDFHSAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLSSKKII-FHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKEL 1805 T+ G L N G+ I ++W++ + + LK + F+F +G++LP F+S G F E WE T P L ++ HAISL D F+ ER L+E + I I++ ++ DL+R + +D + L +E++F NP + + ++V+ L Sbjct: 422 TLEGERTSSELTVVNNGTVAIWYNWRRRPHQDFFQDLKQNKTQR-FYFNNREGVILPGETKHFTFFFKSLNAGIFRESWEFGTHPTLLGGAVLQVTLHAISLTQDIFMDERKLLETKLAAHEAITIAQSVLQDLLRGISTPERTPSPVD---AYLTEEDLFNYRNPRLHYQHQVVQNL 595
BLAST of SMED30013087 vs. UniProt/SwissProt
Match: sp|Q69CM7|MYBPP_RAT (MYCBP-associated protein OS=Rattus norvegicus OX=10116 GN=Mycbpap PE=1 SV=1) HSP 1 Score: 66.6254 bits (161), Expect = 6.796e-10 Identity = 48/182 (26.37%), Postives = 80/182 (43.96%), Query Frame = 3 Query: 1284 TIMGVNEIKNLYFANRGSCTINFSWKKIEIERHLKTDDFH-------SAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSP-VLSSKKIIFHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKEL 1805 T+ G L N G+ I + W+ R + D F F+F +G++LP F+S+ G F E WE T P +L + HAISL D F+ ER L+E + + I++ ++ DL+R + +D + L +E++F NP + + +V+ L Sbjct: 420 TLEGEKTSSELTVVNNGTVAIWYDWR-----RRPQQDFFQDLKQNRTQRFYFNNREGVILPGETKHFTFFFKSRNAGIFMESWEFGTHPTLLGGAALQITLHAISLTQDIFMDERKLLESKLAAHEAVTIAQSVLQDLLRGVSTPERAPSPVD---AYLTEEDLFHYRNPRLHYQHRVVQNL 593
BLAST of SMED30013087 vs. UniProt/SwissProt
Match: sp|Q5SUV2|MYBPP_MOUSE (MYCBP-associated protein OS=Mus musculus OX=10090 GN=Mycbpap PE=1 SV=1) HSP 1 Score: 65.0846 bits (157), Expect = 2.328e-9 Identity = 48/178 (26.97%), Postives = 83/178 (46.63%), Query Frame = 3 Query: 1284 TIMGVNEIKNLYFANRGSCTINFSWKKIEIE---RHLKTDDFHSAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLSSKKII-FHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKEL 1805 T+ G L N G+ I ++W++ + + LK + F+F +G++LP F+S G F E WE T P L ++ HAISL D F+ ER L+E + I I++ ++ DL+R + +D + L +E++F NP + + ++V+ L Sbjct: 422 TLEGERTSSELTVVNNGTVAIWYNWRRRPHQDFFQDLKQNKTQR-FYFNNREGVILPGETKHFTFFFKSLNAGIFRESWEFGTHPTLLGGAVLQVTLHAISLTQDIFMDERKLLETKLAAHEAITIAQSVLQDLLRGISTPERTPSPVD---AYLTEEDLFNYRNPRLHYQHQVVQNL 595
BLAST of SMED30013087 vs. UniProt/SwissProt
Match: sp|Q8TBZ2|MYBPP_HUMAN (MYCBP-associated protein OS=Homo sapiens OX=9606 GN=MYCBPAP PE=1 SV=2) HSP 1 Score: 63.5438 bits (153), Expect = 7.414e-9 Identity = 47/188 (25.00%), Postives = 85/188 (45.21%), Query Frame = 3 Query: 1266 HIHFPATIMGVNEIKNLYFANRGSCTINFSWKKIEIERHLKTDDFH-------SAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLSSKKII-FHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKEL 1805 H+ F T+ G L N G+ I + W+ R + D F F+F +G++LP F+S G F E WE T P L I+ + HA+SL D F ER ++E + + + +E++ +L+ + + +D + L +E++F++ NP + + ++V+ L Sbjct: 433 HLTF-ETLEGEKTSSELTVVNNGTVAIWYDWR-----RQHQPDTFQDLKKNRMQRFYFDNREGVILPGEIKTFTFFFKSLTAGVFREFWEFRTHPTLLGGAILQVNLHAVSLTQDVFEDERKVLESKLTAHEAVTVVREVLQELLMGVLTPERTPSPVD---AYLTEEDLFRHRNPPLHYEHQVVQSL 611
BLAST of SMED30013087 vs. TrEMBL
Match: R7V6V4 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_226990 PE=4 SV=1) HSP 1 Score: 88.5817 bits (218), Expect = 3.728e-14 Identity = 99/408 (24.26%), Postives = 164/408 (40.20%), Query Frame = 3 Query: 1293 GVNEIKNLYFANRGSCTINFSWKKIEIERHLKTDDFH------SAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVL-SSKKIIFHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKELKLFALQNFEID---------------------------------------------WDLSLDSLKQMLREKYDLDSEIAEPMFKEYQEICQGLNVPTINPISVKSDVFNSPFDTGNICFTKLMNSIDEAESFMITIARQLNI-EKFIYNLTEVTPEPAATLPATGTLNKENSSKLPKG--KSDQKT-NAEEPLTAPT---VKEVLHYSFDNVQREAQKCLFREKAYYAVA 2339 G + L AN G+ I FSWK I +T+ F F+F G++LP V IP F+S+ G F E W+L T PV+ +I ++++ D F +R +E+ + QI I+ +++ + + S ID I+ + EIF NPE+++ ++ V++LK Q EID WDLS+ SLK+ + D D E + M + + L+ P ++P + TG + L N ID+ + + L + E+ +L+E +A T +++ PKG +D+K +A+ P AP+ KEV A++ REK + A Sbjct: 502 GCRTLSTLEIANDGTTAIFFSWKSIP-----RTNPFDLLNANVQRFYFNTTNGVILPGDTVKIPFVFKSRNAGVFTEQWQLETRPVVCGGAMLIVTLRGVAVQQDKFHKQRTELEQYLATKQANQIVGSILSEVIEGIRSPERPSSPIDAYIT---EPEIFSRFNPELYYEYQTVQKLKELYRQLEEIDIAAEAAAAAATVEAAAAVKAAEEPKEEEGEEAEEVMAVEERRPDSWDLSVCSLKERIMGIRD-DEEKQQEMLTQLNQFVLQLSFPALSPTQKLM------YSTG---YQLLSNCIDDFSQEALQLRSSLGMPERESDDLSEEPGAISARTQTASTSTRDSRKPAPKGGKGADKKAKDAKPPPKAPSQAQAKEVAKTGKTAAPSGAKRPTTREKDRHTPA 891
BLAST of SMED30013087 vs. TrEMBL
Match: A0A210QY52 (MYCBP-associated protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT00420 PE=4 SV=1) HSP 1 Score: 87.4261 bits (215), Expect = 9.197e-14 Identity = 93/409 (22.74%), Postives = 172/409 (42.05%), Query Frame = 3 Query: 1314 LYFANRGSCTINFSWKKIEIERHLKTDDFHS---AFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVL-SSKKIIFHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKELKLFALQNFEID------WDLSLDSLKQMLREKYDLDSEIAEPMFKEYQEICQGLNVPTINPISVKSDVFNSPFDTGNICFTKLMNSIDE--AESFMITIA-----RQLNI----------EKFIYNLTEVTPEPAATLPA----TGTLNKENSSKLPKGKSDQ----KTNAEEPLTAPTVKEVLHYSFDNVQR-----------EAQKCLFR---EKAYYAVAHVIRNLLSELDHIFDRMV 2393 LY N G+ + + WKK+ + D H F+F + G++LP + P F+S G F E W T P L +I I+L+ D F+ ER +E ++ Q Q+ + ++ D++ + S +D + + +EEIF NP++ +NF +V++LK L+ + WDLS++ LK+ L + + D + + + + P I +K +++ + + L+ ++D+ +S +I R+L I + N + PE PA G KE + PKGK+ Q KT + P P ++ S + +A L+R EK Y ++ + L +++ +F+ ++ Sbjct: 473 LYIVNDGTTAVYYDWKKVPKDNPF--DLVHQNVQRFYFNNSSGVILPGETMKFPFVFKSPSAGVFHEQWRFETRPTLCGGAALIVTLRGIALQEDKFLKEREELERDLQQKQAEQMVRHLMFDIISGIKTPDRPSSPVD---NYITEEEIFARNNPKLHYNFALVQQLKQIYLEMVPEEEREGRIWDLSVEDLKEDLLDMDEDDERKENFLHQLNSNVSKMAYTPH---IPIKKNLY-------KVGYQLLLEAVDKMVGQSMLIRQVMGLPERELTILPEDLSDSRSKSKAENKVKAPPEKGGKAPAKKDEKGAKGKEAAKAEPKGKTPQPPQKKTPSRGPSATPGPGSMMAESRERTMTTPTSTPHSPIPDADPVLYRKYKEKLYTQTTLIMADTLQKMEDVFEEVL 866
BLAST of SMED30013087 vs. TrEMBL
Match: H2ZEA0 (Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1) HSP 1 Score: 84.7297 bits (208), Expect = 1.138e-13 Identity = 70/257 (27.24%), Postives = 120/257 (46.69%), Query Frame = 3 Query: 1266 HIHFPATIMGVNEIKNLYFANRGSCTINFSWKKIEIE---RHLKTDDFHSAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSP-VLSSKKIIFHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKELK-----LFALQNFEI-DWDLSLDSLKQMLREKYDLDSEIAEPMFKEYQEICQGLNVPTINPISVKSDVFNSPF 2006 +HF + G +L N G+ + F W+K+ E LK D F+F +G++LP P F+S G F E WEL+T+P V+ + ++++ D R IEE + + +IIDD++R + ID I+ +EEIFK N ++ E + ELK LFA ++ E +W+LSLD L+ + D ++ E + + L++P + PI + D++++ + Sbjct: 54 RVHFESR-SGERTSSHLVVENNGTTAVYFEWRKVPKEVAFESLKPDTLQR-FYFNTGEGVILPGDTTNFPFVFKSPNPGIFTETWELMTNPAVMGGAALQVVLKGVAVQDDQMEDHRRRIEEELESREAGVVVAKIIDDIIRGIQTPDRCPSPIDAYIT---EEEIFKRKNNGRHYDHETIMELKNIYLQLFAEEDREGREWNLSLDELQSFVSVLPDEGAK--EELLSGLNTVIGRLSMPPLTPI--QQDMYSAGY 301
BLAST of SMED30013087 vs. TrEMBL
Match: W4ZAM1 (Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1) HSP 1 Score: 86.2705 bits (212), Expect = 2.066e-13 Identity = 77/307 (25.08%), Postives = 129/307 (42.02%), Query Frame = 3 Query: 1293 GVNEIKNLYFANRGSCTINFSWKKIEIERHLKTDDFHSA-------FFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLSSKKII-FHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKELKLFALQNF------EIDWDLSLDSLKQMLREKYDL-DSEIAEPMFKEYQEICQGLNVPTINPISVKSDVFNSPFDTGNICFTKLMNSIDEAESFMITIARQLNIEKFIYNLTEVTPEPAATLPATGTL 2168 GV +L N G+ TI + WKKI K + F F+F + G++LP ++ P F+S G F E W+L T PV+ + ++L+ D + +R IEE + + I+++LV + ID I+ DEE F+ LNP + + E+V +LK ++ F + +WDL + +K RE + D E+ E + + L P PI + + + +M ++D ++T A + + I E P+ A L A L Sbjct: 518 GVRTTSHLELVNDGTTTIYYDWKKIP-----KLNPFEGCSTPRIQRFYFNTSSGVLLPGESLRFPFVFKSPNAGIFTESWQLDTRPVVCGGGALQVTLRGVALQEDKNLKQRKDIEEELFAKQAQVAVRRILEELVDGIRTPERAHSPIDAYIT---DEETFERLNPGMHFANEVVSQLKQLHVEQFDEEEREDKEWDLDVSKMK---REFLAIEDEEMREELLNRMNQAVTSLYFPPFTPIQ---------HEMYRVAYNLMMENVDN----IVTQANTIRVNMGIPEKVEELPDTAEILAANDAL 800
BLAST of SMED30013087 vs. TrEMBL
Match: A0A2R2MRE5 (MYCBP-associated protein-like isoform X2 OS=Lingula unguis OX=7574 GN=LOC106180241 PE=4 SV=1) HSP 1 Score: 85.8853 bits (211), Expect = 2.486e-13 Identity = 129/564 (22.87%), Postives = 223/564 (39.54%), Query Frame = 3 Query: 735 FSSHQDEIFKI--ILPCFQNGTGAPITHSIWPVQEETKSKLHVQRYKWKPHIPLQTSMTGYHELILH----EMNETTKGLESSEKIKI------KNEVLNETKYQ--RFLKDYHYHTPDISELIICS-----KTYQKEQPQKENTTLSNFXXXXXXXXXXNTKSVRGAILLINDELIFERLNDKDQILSKP----------------NDQY---LHIHFPATIM-----GVNEIKNLYFANRGSCTINFSWKKIEIERHLKTDDFH------SAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVL-SSKKIIFHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKEL-----KLFALQNF-EIDWDLSLDSLKQMLREKYDLDSEIAEPMFKEYQEICQGLNVPTINPISVKSDVFNSPFDTGNICFTKLMNSIDE--AESFMI----TIARQLNIEKFIYNLTEVTP------EPAATLPAT-GTLNKENSSKLPKGKSDQKT 2219 + Q+E + I +P G G + W QE L H+ L + GY I H M + KG+ S+ ++ L + K Q + + H PD+S L I K KE ++E T+S FE+ ++ D+ + L D ++ +P D Y F A + G + L N G+ +I + WKK+ K++ F F+F + G++LP + P F+S G F E W+ T PV+ ++ ++L+ D F +R IE+ + QI ++I+DD+V + +D I+ E+IF+ N + ++ E V EL LF + +++WDLSL++L++ + E D D E + ++ L+ P +PI + +++ + + L+ +ID +S MI + + E ++T TP P A+ A G +K++S KG D KT Sbjct: 257 YRKKQEERYLIDRTIPSMDYGKGYRVGSEFWKQQERIGDDLEGM------HMTLTQTERGYPPPIEHVGVPTMIKEEKGVYWSKPSTPVHYPWDRSAFLKQRKQQLQTVMDELDPHKPDMSSLAITGFKQGPKLVDKEDEREEPRTMSEFEDS-----------------VLEDQENLDPLKDHPDVVPEPIFGPSILFAGRPARWTGDSYSFQGQQAFEARVTFEGYSGQRKTSFLEVVNNGTTSIYYDWKKLP-----KSNPFDIVNSRVQRFYFNTSGGVILPGDTMKFPFVFKSPNAGVFTEQWQFETRPVVCGGASLVVTLRGVALQEDKFKHQRSEIEKELAHKQAEQIIRQIVDDVVEGVQTPDRARSPVDAYIT---REDIFERKNQGMHFSNETVTELNELYKNLFPEEERDDVEWDLSLETLREAIME-MDEDDEQKQEFLQQLNSAVISLSFPPFSPI--QQEMY-------KVAYQALLETIDSIVGQSMMIRQVLGLPEKEFEEFPEGDITHSTPHTSHSERPGASQKAGRGQDSKKDSKAGQKGGKDAKT 779
BLAST of SMED30013087 vs. Ensembl Nematostella
Match: EDO31470 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SXU7]) HSP 1 Score: 68.5514 bits (166), Expect = 1.012e-11 Identity = 51/188 (27.13%), Postives = 82/188 (43.62%), Query Frame = 3 Query: 1314 LYFANRGSCTINFSWKKIEIERHLKTD--DFHSAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLSSKKII-FHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKELKLFALQNFEIDWDLSLDSLKQ 1868 L+ N G+ I FSWK++ L T F+F N G++LP + P F+S G F E WEL+T PVL I I++ D + R IE+ + I+ + I+ +++ + +D I++ +E ++L E+F E E WDLS+ L+Q Sbjct: 489 LHICNDGTTAIYFSWKRVPKANTLGTKLAGRTQRFYFDTNSGVILPGETLKFPFIFKSLNAGIFSENWELLTEPVLCGGGAIRVCLRGIAIEEDVYADARREIEKELAHREAIEAAARILQEILDGVRTPERSRSPVDAYITMHFQDEAVQSLK-EIFVEVEPGAE------------WDLSVGGLQQ 663
BLAST of SMED30013087 vs. Planmine SMEST
Match: SMESG000023252.1 (SMESG000023252.1) HSP 1 Score: 1438.71 bits (3723), Expect = 0.000e+0 Identity = 744/752 (98.94%), Postives = 747/752 (99.34%), Query Frame = 3 Query: 141 MINRRKSILNKTRRDSVRSAALKLLQQKEDRENENANSFLRQRRYEDLDNLKIKEDDLNKNSNNTFFSLNFKSRNCAVRRLIPLDQRRXXXXXXXXXXXXXXDITTDIQMLSSLNDETIVKVYENRLKMETFSPNEVQEKSFLTTNMSDRPVEENSFKSLENWKLRMLERRKLKTSLAKRLQTNPESLLISKSAQSSLFSSHQDEIFKIILPCFQNGTGAPITHSIWPVQEETKSKLHVQRYKWKPHIPLQTSMTGYHELILHEMNETTKGLESSEKIKIKNEVLNETKYQRFLKDYHYHTPDISELIICSKTYQKEQPQKENTTLSNFXXXXXXXXXXNTKSVRGAILLINDELIFERLNDKDQILSKPNDQYLHIHFPATIMGVNEIKNLYFANRGSCTINFSWKKIEIERHLKTDDFHSAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLSSKKIIFHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKELKLFALQNFEIDWDLSLDSLKQMLREKYDLDSEIAEPMFKEYQEICQGLNVPTINPISVKSDVFNSPFDTGNICFTKLMNSIDEAESFMITIARQLNIEKFIYNLTEVTPEPAATLPATGTLNKENSSKLPKGKSDQKTNAEEPLTAPTVKEVLHYSFDNVQREAQKCLFREKAYYAVAHVIRNLLSELDHIFDRMVN 2396 MINRRKSILNKTRRDSVRSAALKLLQQKEDRENENANSFLRQRRYEDLDNLKIKEDDLNKNSNNTFFSLNFKSRNCAVRRLIPLDQRRLHLKGMMLKKFLKKDITTDIQMLSSLNDET VKVYENRLKMETFSPNEVQEK FLTTNMSDRPVEENSFKSLENWKLRMLERRKLKTSLAKRLQTNPESLLISKSAQSSLFSSHQDEIFKIILPCFQNGTGAPITHSIWPVQEETKSKLHVQ YKWKPHIPLQTSMTGYHELILHEMNETTK LESSEKIKIKNEVLN+TKYQRFLKDYHYHTPDISELIICSKTYQKEQP+KENTTLSNFEEEEILTELENTKSVRG ILLINDELIFERLNDKDQILSKPNDQYLHIHFPATIMGVNEIKNLYFANRGSCTINFSWKKIEIERHLKTDDFHSAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVL+SKKIIFHFHAISLRPDYFIFERHLIEENVHQSIIIQISKEIIDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKELKLFALQNFEIDWDLSLDSLKQMLREKYDLDSEIAEPMFKEYQEICQGLNVPTINPISVKSDVFNSPFDTGNICFTKLMNSIDEAESFMITIARQLNIEKFIYNLTEVTPEPAATLPATGTLNKENSSKLPKGKSDQKTNAEEPLTAPTVKEVLHYSFDNVQREAQKCLFREKAYYAVAHVIRNLLSELDHIFDRMVN Sbjct: 1 MINRRKSILNKTRRDSVRSAALKLLQQKEDRENENANSFLRQRRYEDLDNLKIKEDDLNKNSNNTFFSLNFKSRNCAVRRLIPLDQRRLHLKGMMLKKFLKKDITTDIQMLSSLNDETNVKVYENRLKMETFSPNEVQEKKFLTTNMSDRPVEENSFKSLENWKLRMLERRKLKTSLAKRLQTNPESLLISKSAQSSLFSSHQDEIFKIILPCFQNGTGAPITHSIWPVQEETKSKLHVQCYKWKPHIPLQTSMTGYHELILHEMNETTKDLESSEKIKIKNEVLNKTKYQRFLKDYHYHTPDISELIICSKTYQKEQPKKENTTLSNFEEEEILTELENTKSVRGPILLINDELIFERLNDKDQILSKPNDQYLHIHFPATIMGVNEIKNLYFANRGSCTINFSWKKIEIERHLKTDDFHSAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLTSKKIIFHFHAISLRPDYFIFERHLIEENVHQSIIIQISKEIIDDLVRYAVNRTKLSIEIDCDISILNDEEIFKNLNPEVFWNFEIVKELKLFALQNFEIDWDLSLDSLKQMLREKYDLDSEIAEPMFKEYQEICQGLNVPTINPISVKSDVFNSPFDTGNICFTKLMNSIDEAESFMITIARQLNIEKFIYNLTEVTPEPAATLPATGTLNKENSSKLPKGKSDQKTNAEEPLTAPTVKEVLHYSFDNVQREAQKCLFREKAYYAVAHVIRNLLSELDHIFDRMVN 752
BLAST of SMED30013087 vs. Planmine SMEST
Match: SMESG000045572.1 (SMESG000045572.1) HSP 1 Score: 61.2326 bits (147), Expect = 2.716e-9 Identity = 55/194 (28.35%), Postives = 94/194 (48.45%), Query Frame = 3 Query: 1305 IKNLYFANRGSCTINFSWKKIEIERHLKTDDFH-SAFFFKINKGIVLPDSNVIIPVTFRSKFLGFFDEMWELVTSPVLS-SKKIIFHFHAISLRPDYFIFERHLIEENVHXXXXXXXXXXXXDDLVRYAVNRTKLSIEIDCDISILN-DEEIFKNLNPEVFWNFEIVKELK----------LFALQNFEIDWDL 1847 ++ L F NRG+ I + WKKIE ++ + F+FK GI+LP+ ++ +P+ F+S G F E+W++ T P+L+ I+ I+L+ D R I+ + + IS+ I+D++V+ N K ++ + S N D + F N F+ E + LK L A N+ +D L Sbjct: 362 VEQLLFQNRGTSIIRYWWKKIESFDPFNLENKNIRRFYFKTEDGILLPNEDLNLPIIFKSAKSGIFTEVWKIETIPLLANGNDIVIILKGIALQNDVTAAIRQGIDSYMKYQVADNISRTILDEIVQ---NIGKTDLQHHPEDSFENTDRDQFVQKNNGFFYEIENINSLKKLNEEIAGKELNAYDNWRMDCGL 552 The following BLAST results are available for this feature:
BLAST of SMED30013087 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 4
BLAST of SMED30013087 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30013087 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30013087 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of SMED30013087 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 2
BLAST of SMED30013087 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of SMED30013087 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 3
BLAST of SMED30013087 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30013087 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30013087 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30013087 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30013087 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of SMED30013087 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30013087 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30013087 ID=SMED30013087|Name=SMED30013087|organism=Schmidtea mediterranea sexual|type=transcript|length=2420bpback to top protein sequence of SMED30013087-orf-1 >SMED30013087-orf-1 ID=SMED30013087-orf-1|Name=SMED30013087-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=753bp MINRRKSILNKTRRDSVRSAALKLLQQKEDRENENANSFLRQRRYEDLDNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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