Succinate dehydrogenase [ubiquinone] cytochrome b small subunit

Overview
NameSuccinate dehydrogenase [ubiquinone] cytochrome b small subunit
Smed IDSMED30013079
Length (bp)653
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit (SMED30013079) t-SNE clustered cells

Violin plots show distribution of expression levels for Succinate dehydrogenase [ubiquinone] cytochrome b small subunit (SMED30013079) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit (SMED30013079) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Succinate dehydrogenase [ubiquinone] cytochrome b small subunit (SMED30013079) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30013079

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30013079h1SMcG0014804 dd_Smed_v4_982_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30013079h1SMcG0014804 dd_Smed_v4_982_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30013079h1SMcG0014804 dd_Smed_v4_982_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30013079h1SMcG0014804 dd_Smed_v4_982_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30013079h1SMcG0014804 dd_Smed_v4_982_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30013079h1SMcG0014804 dd_Smed_v4_982_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Human
Match: SDHD (succinate dehydrogenase complex subunit D [Source:HGNC Symbol;Acc:HGNC:10683])

HSP 1 Score: 71.2478 bits (173), Expect = 2.028e-15
Identity = 39/103 (37.86%), Postives = 58/103 (56.31%), Query Frame = 1 
Query:  229 PANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
             A+ HWT ER+V+V +L + PAA++     M   +   ++LH  WGL  V+ DYV    +G  LQ   +     +SA+ FAG  YF ++D+G  KAV  LW +
Sbjct:   22 AASLHWTSERVVSVLLLGLLPAAYLNPCSAMDYSLAAALTLHGHWGLGQVVTDYV----HGDALQKAAKAGLLALSALTFAGLCYFNYHDVGICKAVAMLWKL 120
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Human
Match: SDHD (succinate dehydrogenase complex subunit D [Source:HGNC Symbol;Acc:HGNC:10683])

HSP 1 Score: 70.8626 bits (172), Expect = 6.216e-15
Identity = 39/102 (38.24%), Postives = 58/102 (56.86%), Query Frame = 1 
Query:  232 ANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            A+ HWT ER+V+V +L + PAA++     M   +   ++LH  WGL  V+ DYV    +G  LQ   +     +SA+ FAG  YF ++D+G  KAV  LW +
Sbjct:   62 ASLHWTSERVVSVLLLGLLPAAYLNPCSAMDYSLAAALTLHGHWGLGQVVTDYV----HGDALQKAAKAGLLALSALTFAGLCYFNYHDVGICKAVAMLWKL 159
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Celegans
Match: sdhd-1 (Putative succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:O62215])

HSP 1 Score: 63.929 bits (154), Expect = 7.177e-13
Identity = 37/124 (29.84%), Postives = 61/124 (49.19%), Query Frame = 1 
Query:  166 ISTSTVANTKLGTAPTAGKIMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            IST   A + L     A K+   + H+ +ER+ AV MLPI PA++ +    M  ++   ++LH  WG+ GV+ DY      G            +I+ +     L+F  ND+G  KA + ++S+
Sbjct:   24 ISTIVRATSTLNDG--ASKVPDHSMHFKLERLWAVGMLPILPASYFIHGPVMDAVLTVALTLHIHWGIHGVVYDYARPYVIGEAAAKAAHVGVYLITGLLLGALLHFNTNDVGITKAFELVFSL 145
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Fly
Match: SdhD (gene:FBgn0039112 transcript:FBtr0084466)

HSP 1 Score: 74.3294 bits (181), Expect = 2.533e-16
Identity = 37/133 (27.82%), Postives = 71/133 (53.38%), Query Frame = 1 
Query:  139 SFSKFPSRVISTSTVANTKLGTAPTAGKIMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            + +K  + V+   +V+  ++ +A ++  ++     WT+ERIV+  +L + PAA+I  +Q +  L+  +V +H  WG++ ++ DY+     G  L  +   A  +IS     G  YF  ND+G    +K+ W+I
Sbjct:   47 AVAKIVAPVVREISVSAPRMASAGSSHTLL-----WTVERIVSAGLLAVIPAAFIAPSQVLDALMAISVVIHTHWGVEAMVVDYMRPSVVGNVLPKVAHIALIIISVATLGGLFYFIQNDVGLANGIKRFWAI 174
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Zebrafish
Match: sdhdb (succinate dehydrogenase complex, subunit D, integral membrane protein b [Source:NCBI gene;Acc:445500])

HSP 1 Score: 70.8626 bits (172), Expect = 4.477e-15
Identity = 35/101 (34.65%), Postives = 55/101 (54.46%), Query Frame = 1 
Query:  232 ANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWS 534
            A  HWT ERI+++++L + P A+   +  +   +   ++LH  WGL  V+ DYV    +G     +      V+S + FAG  YF ++D+G  KAV  LWS
Sbjct:   61 ATMHWTGERILSIALLSLAPVAYFCPSPAVDYSLAAALTLHGHWGLGQVVTDYV----HGDAKIKMANAGLFVLSTVTFAGLCYFNYHDVGICKAVALLWS 157
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Zebrafish
Match: sdhda (succinate dehydrogenase complex, subunit D, integral membrane protein a [Source:NCBI gene;Acc:436762])

HSP 1 Score: 65.855 bits (159), Expect = 2.470e-13
Identity = 40/140 (28.57%), Postives = 67/140 (47.86%), Query Frame = 1 
Query:  115 FMYKTGISSFSKFPSRVISTSTVANTKLGTAPTAGKIMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWS 534
             +  T +S  S  P       T    ++ TA ++  I  A+ HWT ER++ + +L + PAA++     +   +   +++H  WG+  VL DYV    +G     + +    ++S   F G  YF +ND+G  KAV  LW 
Sbjct:   19 LLCHTQLSRGSACPRIGDVDYTEPAGRIHTASSSTGI-SASQHWTGERVMTLVLLGMAPAAYLCPGPVVDYSIAAALTMHGHWGIGQVLTDYV----HGEVKIKLAKVGIFLLSTATFFGLCYFNYNDVGLCKAVVMLWQ 153
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Xenopus
Match: sdhd (succinate dehydrogenase complex subunit D, integral membrane protein [Source:NCBI gene;Acc:394986])

HSP 1 Score: 77.0258 bits (188), Expect = 2.010e-17
Identity = 43/130 (33.08%), Postives = 68/130 (52.31%), Query Frame = 1 
Query:  154 PSRVISTSTVANTKLGTAPT--AGKIMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            P        V  +++ T+P   AG    A+ HWT ER ++V++L + PAA++     M   +   ++LH  WGL  V+ DYV    +G     +   +   +SA+ FAG  YF ++D+G  KAV  LWS+
Sbjct:   28 PCLTQDHHMVQTSQIHTSPNHHAGS-KAASMHWTSERALSVALLGLLPAAYLYPGAAMDYSLAAALTLHGHWGLGQVVTDYV----HGDAKIKMANTSLFALSALTFAGLCYFNYHDVGICKAVSMLWSL 152
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Xenopus
Match: sdhd (succinate dehydrogenase complex subunit D, integral membrane protein [Source:NCBI gene;Acc:394986])

HSP 1 Score: 77.0258 bits (188), Expect = 2.360e-17
Identity = 43/130 (33.08%), Postives = 68/130 (52.31%), Query Frame = 1 
Query:  154 PSRVISTSTVANTKLGTAPT--AGKIMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            P        V  +++ T+P   AG    A+ HWT ER ++V++L + PAA++     M   +   ++LH  WGL  V+ DYV    +G     +   +   +SA+ FAG  YF ++D+G  KAV  LWS+
Sbjct:   25 PCLTQDHHMVQTSQIHTSPNHHAGS-KAASMHWTSERALSVALLGLLPAAYLYPGAAMDYSLAAALTLHGHWGLGQVVTDYV----HGDAKIKMANTSLFALSALTFAGLCYFNYHDVGICKAVSMLWSL 149
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Mouse
Match: Sdhd (succinate dehydrogenase complex, subunit D, integral membrane protein [Source:MGI Symbol;Acc:MGI:1914175])

HSP 1 Score: 75.0998 bits (183), Expect = 1.296e-16
Identity = 36/102 (35.29%), Postives = 57/102 (55.88%), Query Frame = 1 
Query:  232 ANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            A+ HWT ER+V+V +L + PA ++     +   +   ++LH+ WGL  V+ DYV    +G  L    R     +SA+ FAG  YF ++D+G  +AV  LW +
Sbjct:   62 ASLHWTSERVVSVLLLGLIPAGYLNPCSVVDYSLAAALTLHSHWGLGQVVTDYV----HGDTLPKAARAGLLALSALTFAGLCYFNYHDVGICRAVAMLWKL 159
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. UniProt/SwissProt
Match: sp|Q5G2C6|DHSD_SHEEP (Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial OS=Ovis aries OX=9940 GN=SDHD PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.045e-17
Identity = 39/102 (38.24%), Postives = 60/102 (58.82%), Query Frame = 1 
Query:  232 ANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            A+ HWT ER+V+V +L + PAA++     M   +  T++LH+ WG+  V+ DYV    +G  +Q   +    V+SA  FAG  YF ++D+G  KAV  LW +
Sbjct:   61 ASLHWTGERVVSVLLLGLIPAAYLNPCSAMDYSLAATLTLHSHWGIGQVVTDYV----HGDAVQKAAKTGLLVLSAFTFAGLCYFNYHDVGICKAVAMLWKL 158
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. UniProt/SwissProt
Match: sp|Q95123|DHSD_BOVIN (Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial OS=Bos taurus OX=9913 GN=SDHD PE=2 SV=2)

HSP 1 Score: 79.337 bits (194), Expect = 1.626e-17
Identity = 39/102 (38.24%), Postives = 60/102 (58.82%), Query Frame = 1 
Query:  232 ANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            A+ HWT ER+V+V +L + PAA++     M   +  T++LH+ WG+  V+ DYV    +G  +Q   +    V+SA  FAG  YF ++D+G  KAV  LW +
Sbjct:   61 ASLHWTGERVVSVLLLGLIPAAYLNPCSAMDYSLAATLTLHSHWGIGQVVTDYV----HGDAVQKAAKTGLLVLSAFTFAGLCYFNYHDVGICKAVAMLWKL 158
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. UniProt/SwissProt
Match: sp|Q6P355|DHSD_XENTR (Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial OS=Xenopus tropicalis OX=8364 GN=sdhd PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.065e-16
Identity = 43/130 (33.08%), Postives = 68/130 (52.31%), Query Frame = 1 
Query:  154 PSRVISTSTVANTKLGTAPT--AGKIMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            P        V  +++ T+P   AG    A+ HWT ER ++V++L + PAA++     M   +   ++LH  WGL  V+ DYV    +G     +   +   +SA+ FAG  YF ++D+G  KAV  LWS+
Sbjct:   28 PCLTQDHHMVQTSQIHTSPNHHAGS-KAASMHWTSERALSVALLGLLPAAYLYPGAAMDYSLAAALTLHGHWGLGQVVTDYV----HGDAKIKMANTSLFALSALTFAGLCYFNYHDVGICKAVSMLWSL 152
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. UniProt/SwissProt
Match: sp|Q6AZV0|DHSDB_XENLA (Succinate dehydrogenase [ubiquinone] cytochrome b small subunit B, mitochondrial OS=Xenopus laevis OX=8355 GN=sdhd-b PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 2.441e-16
Identity = 42/121 (34.71%), Postives = 67/121 (55.37%), Query Frame = 1 
Query:  181 VANTKLGTAPT--AGKIMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            V  +++ T+P   AG    A+ HWT ER ++V++L + PAA++     M   +   ++LH  WGL  V+ DYV    +G     +   +   +SA+ FAG  YF ++D+G  KAV  LWS+
Sbjct:   37 VQTSQIHTSPNHHAGS-KAASMHWTGERALSVALLGLLPAAYLYPGAAMDYSLAAALTLHGHWGLGQVVTDYV----HGETKIKMANTSLFALSALTFAGLCYFNYHDVGICKAVAMLWSL 152
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. UniProt/SwissProt
Match: sp|Q6PCT8|DHSD_RAT (Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial OS=Rattus norvegicus OX=10116 GN=Sdhd PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 4.786e-16
Identity = 35/102 (34.31%), Postives = 58/102 (56.86%), Query Frame = 1 
Query:  232 ANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            A+ HWT ER+V+V +L + PA ++     +   +   ++LH+ WG+  V+ DYV    +G  LQ   +     +SA+ FAG  YF ++D+G  +AV  LW +
Sbjct:   62 ASLHWTSERVVSVLLLGLIPAGYLNPCSVVDYSLAAALTLHSHWGIGQVVTDYV----HGDALQKATKAGLLAVSALTFAGLCYFNYHDVGICRAVAMLWKL 159
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. TrEMBL
Match: A0A5J4NCU3 (Succinate dehydrogenase [ubiquinone] cytochrome b small subunit OS=Paragonimus westermani OX=34504 GN=DEA37_0014703 PE=3 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 1.861e-44
Identity = 76/135 (56.30%), Postives = 95/135 (70.37%), Query Frame = 1 
Query:  145 SKFPS---RVISTSTVANTKLGTAPTAGKI-MPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            S+ PS   R I   TV   KLGT PT  K  M  + HWTIERIVAVSMLP+YP A  ++   M+ +VV++V++HA+WGLDGV+KDY  ERRYGP L P +R  W V  A GFAG LYF +ND+GF+ AVKKLW++
Sbjct:   19 SRTPSLQCRTIFVGTVDRAKLGTLPTPVKSGMLPSIHWTIERIVAVSMLPLYPIALYVDGPAMNFIVVSSVTMHAYWGLDGVIKDYAFERRYGPALMPALRTIWAVFCATGFAGLLYFNYNDVGFITAVKKLWAM 153
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. TrEMBL
Match: C7TZ33 (Succinate dehydrogenase [ubiquinone] cytochrome b small subunit OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 2.617e-44
Identity = 72/130 (55.38%), Postives = 94/130 (72.31%), Query Frame = 1 
Query:  157 SRVISTSTVANT-KLGTAPTAGK--IMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
             R I+ S V  + KLGTAP   K  ++P + HWTIERI+A  MLP+YP A  ++   M+ +VVT VS+H++WG DGV+KDY  ERRYGP L PI+R  WK++S  GFAG LYF FND+GF+ AVKKLW++
Sbjct:   23 CRPITQSRVLESMKLGTAPAVQKTGLLP-SVHWTIERIIATGMLPLYPIALYMDTPIMNFIVVTAVSMHSYWGFDGVIKDYAFERRYGPALMPILRTLWKIMSGCGFAGLLYFNFNDVGFITAVKKLWAL 151
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. TrEMBL
Match: A0A4Z2DAA2 (Succinate dehydrogenase [ubiquinone] cytochrome b small subunit OS=Schistosoma japonicum OX=6182 GN=EWB00_003141 PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 9.443e-44
Identity = 68/117 (58.12%), Postives = 88/117 (75.21%), Query Frame = 1 
Query:  193 KLGTAPTAGK--IMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            KLGTAP   K  ++P + HWTIERI+A  MLP+YP A  ++   M+ +VVT VS+H++WG DGV+KDY  ERRYGP L PI+R  WK++S  GFAG LYF FND+GF+ AVKKLW++
Sbjct:   36 KLGTAPAVQKTGLLP-SVHWTIERIIATGMLPLYPIALYMDTPIMNFIVVTAVSMHSYWGFDGVIKDYAFERRYGPALMPILRTLWKIMSGCGFAGLLYFNFNDVGFITAVKKLWAL 151
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. TrEMBL
Match: G4V6D5 (Succinate dehydrogenase [ubiquinone] cytochrome b small subunit OS=Schistosoma mansoni OX=6183 GN=Smp_037910 PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 9.739e-44
Identity = 69/127 (54.33%), Postives = 92/127 (72.44%), Query Frame = 1 
Query:  163 VISTSTVANTKLGTAPTAGK--IMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            +     + + KLGTAP   +  ++P + HWT ERI+A SMLP+YP A  ++   M+L+VVT VS+H++WG DGV+KDY  ERRYGP L PI+R  WKV++  GFAG LYF FNDIGF+ AVKKLW++
Sbjct:   27 ITQDHALKSNKLGTAPAVQRTGLLP-SYHWTFERILAASMLPLYPIALYMDTPMMNLIVVTAVSMHSYWGFDGVIKDYAFERRYGPALMPILRTLWKVMAGFGFAGLLYFNFNDIGFISAVKKLWAL 152
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. TrEMBL
Match: A0A183KG10 (Succinate dehydrogenase [ubiquinone] cytochrome b small subunit OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS13957 PE=3 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 1.394e-43
Identity = 68/127 (53.54%), Postives = 92/127 (72.44%), Query Frame = 1 
Query:  163 VISTSTVANTKLGTAPTAGK--IMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            +    T+ + KLGTAP   +  ++P+  HWT ERI+A SMLP+YP A  ++   M+ +VVT VS+H++WG DGV+KDY  ERRYGP L PI+R  WKV++  G+AG LYF FNDIGF+ AVKKLW++
Sbjct:   27 ITQDHTLNSNKLGTAPAVQRTGLLPS-YHWTFERILAASMLPLYPVALYMDTPMMNFIVVTAVSMHSYWGFDGVIKDYAFERRYGPALMPILRTLWKVMAGCGYAGLLYFNFNDIGFISAVKKLWAL 152
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Cavefish
Match: sdhdb (succinate dehydrogenase complex subunit D [Source:NCBI gene;Acc:103030352])

HSP 1 Score: 73.559 bits (179), Expect = 3.796e-16
Identity = 37/101 (36.63%), Postives = 56/101 (55.45%), Query Frame = 1 
Query:  232 ANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWS 534
            A+ HWT ERIV++++L + P A+      M   +   ++LH  WGL  V+ DYV    +G     +      ++SA+ FAG  YF ++D+G  KAV  LWS
Sbjct:   62 ASLHWTGERIVSIALLGLGPVAYFYPGPVMDYSLAAVLTLHGHWGLGQVVTDYV----HGDAKIKLANTGLFLLSAVTFAGLCYFNYHDVGICKAVALLWS 158
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Sea Lamprey
Match: sdhda (succinate dehydrogenase complex, subunit D, integral membrane protein a [Source:NCBI gene;Acc:436762])

HSP 1 Score: 78.9518 bits (193), Expect = 1.815e-19
Identity = 42/118 (35.59%), Postives = 61/118 (51.69%), Query Frame = 1 
Query:  187 NTKLGTAPTA-GKIMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
               L T P A G +  A+ HW+ ER+V+++   + PAA           +  T++LH+ WGL  V+ DYV    +G       R     ISA+ FAG  YF ++D+G  KAV  LWS+
Sbjct:    1 TAALHTTPRAHGALGSASAHWSAERVVSIAAFTLIPAAVAFPGGVTDYAIAATLTLHSHWGLGQVITDYV----HGKATLGAARVGLASISAVTFAGLCYFNYHDVGICKAVAMLWSL 114
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Nematostella
Match: EDO46088 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR14])

HSP 1 Score: 65.0846 bits (157), Expect = 4.200e-14
Identity = 33/100 (33.00%), Postives = 48/100 (48.00%), Query Frame = 1 
Query:  238 THWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            +HW  ER V++++L + P   +     +   +   +  H  WG+  VLKDY       P L       W V+S +  AG  YF +ND+G  KAV  LW I
Sbjct:    2 SHWLNERYVSIALLALIPVGIVYPCAAVDWGLAVLIPFHNHWGMHNVLKDY--SHHINPKLPKPANITWLVLSFLQLAGLCYFNYNDVGICKAVSMLWDI 99
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Medaka
Match: sdhda (succinate dehydrogenase complex subunit D [Source:NCBI gene;Acc:101167814])

HSP 1 Score: 74.7146 bits (182), Expect = 1.206e-16
Identity = 35/101 (34.65%), Postives = 57/101 (56.44%), Query Frame = 1 
Query:  232 ANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWS 534
            A+ HWT ER++++++L + P A+      +   +   ++LH  WG+D VL DYV    +G     + +    ++S + FAG  YF +ND+G  KAV  LWS
Sbjct:   61 ASLHWTAERVLSIALLAMGPVAYYSPGPVIDYSLAAALTLHGHWGIDQVLTDYV----HGDTKVKMAKAGLFLLSTVTFAGLCYFNYNDVGICKAVALLWS 157
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Planmine SMEST
Match: SMESG000075449.1 (SMESG000075449.1)

HSP 1 Score: 306.99 bits (785), Expect = 5.587e-108
Identity = 149/149 (100.00%), Postives = 149/149 (100.00%), Query Frame = 1 
Query:   91 MNQIRIYQFMYKTGISSFSKFPSRVISTSTVANTKLGTAPTAGKIMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            MNQIRIYQFMYKTGISSFSKFPSRVISTSTVANTKLGTAPTAGKIMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI
Sbjct:    1 MNQIRIYQFMYKTGISSFSKFPSRVISTSTVANTKLGTAPTAGKIMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 149
[Close]
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Planmine SMEST
Match: SMESG000075449.1 (SMESG000075449.1)

HSP 1 Score: 286.96 bits (733), Expect = 3.042e-100
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 1 
Query:  118 MYKTGISSFSKFPSRVISTSTVANTKLGTAPTAGKIMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 537
            MYKTGISSFSKFPSRVISTSTVANTKLGTAPTAGKIMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI
Sbjct:    1 MYKTGISSFSKFPSRVISTSTVANTKLGTAPTAGKIMPANTHWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDYVMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI 140
The following BLAST results are available for this feature:
BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
SDHD2.028e-1537.86succinate dehydrogenase complex subunit D [Source:... [more]
SDHD6.216e-1538.24succinate dehydrogenase complex subunit D [Source:... [more]
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BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
sdhd-17.177e-1329.84Putative succinate dehydrogenase [ubiquinone] cyto... [more]
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BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
SdhD2.533e-1627.82gene:FBgn0039112 transcript:FBtr0084466[more]
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BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
sdhdb4.477e-1534.65succinate dehydrogenase complex, subunit D, integr... [more]
sdhda2.470e-1328.57succinate dehydrogenase complex, subunit D, integr... [more]
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BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
sdhd2.010e-1733.08succinate dehydrogenase complex subunit D, integra... [more]
sdhd2.360e-1733.08succinate dehydrogenase complex subunit D, integra... [more]
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BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Sdhd1.296e-1635.29succinate dehydrogenase complex, subunit D, integr... [more]
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BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5G2C6|DHSD_SHEEP1.045e-1738.24Succinate dehydrogenase [ubiquinone] cytochrome b ... [more]
sp|Q95123|DHSD_BOVIN1.626e-1738.24Succinate dehydrogenase [ubiquinone] cytochrome b ... [more]
sp|Q6P355|DHSD_XENTR1.065e-1633.08Succinate dehydrogenase [ubiquinone] cytochrome b ... [more]
sp|Q6AZV0|DHSDB_XENLA2.441e-1634.71Succinate dehydrogenase [ubiquinone] cytochrome b ... [more]
sp|Q6PCT8|DHSD_RAT4.786e-1634.31Succinate dehydrogenase [ubiquinone] cytochrome b ... [more]
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BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J4NCU31.861e-4456.30Succinate dehydrogenase [ubiquinone] cytochrome b ... [more]
C7TZ332.617e-4455.38Succinate dehydrogenase [ubiquinone] cytochrome b ... [more]
A0A4Z2DAA29.443e-4458.12Succinate dehydrogenase [ubiquinone] cytochrome b ... [more]
G4V6D59.739e-4454.33Succinate dehydrogenase [ubiquinone] cytochrome b ... [more]
A0A183KG101.394e-4353.54Succinate dehydrogenase [ubiquinone] cytochrome b ... [more]
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BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
sdhdb3.796e-1636.63succinate dehydrogenase complex subunit D [Source:... [more]
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BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
sdhda1.815e-1935.59succinate dehydrogenase complex, subunit D, integr... [more]
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BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO460884.200e-1433.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
sdhda1.206e-1634.65succinate dehydrogenase complex subunit D [Source:... [more]
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BLAST of Succinate dehydrogenase [ubiquinone] cytochrome b small subunit vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000075449.15.587e-108100.00SMESG000075449.1[more]
SMESG000075449.13.042e-100100.00SMESG000075449.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013079 ID=SMED30013079|Name=Succinate dehydrogenase [ubiquinone] cytochrome b small subunit|organism=Schmidtea mediterranea sexual|type=transcript|length=653bp
GTTTCGTATATTTTGTTTAAGTAATTCAAATGCCTAATTGAACTCTTAAT
TTTCTACTAATAAATATAAATCACGTCTGCTTTCAATATCATGAATCAAA
TTCGAATTTATCAGTTTATGTATAAAACTGGAATTTCCAGCTTTTCGAAA
TTTCCGTCGCGTGTTATTTCAACATCAACGGTTGCAAACACAAAATTGGG
AACTGCTCCAACTGCCGGTAAAATTATGCCTGCTAATACCCACTGGACAA
TTGAAAGAATAGTAGCAGTCAGTATGCTACCTATTTATCCAGCAGCTTGG
ATACTTGAAAATCAATTTATGAGTTTATTAGTCGTTACGACAGTTTCATT
GCATGCATTTTGGGGCTTGGACGGGGTCCTTAAAGACTACGTCATGGAGC
GCCGATATGGGCCGTATTTGCAGCCGATAATGAGGAATGCCTGGAAAGTC
ATTTCAGCAATCGGATTCGCAGGATTCCTCTATTTCGAGTTCAATGATAT
AGGATTTGTGAAAGCCGTCAAGAAATTGTGGTCGATTTGAATCGGATTTC
CAATAATTTATGCATATATATGTGTGTGTGTGTGATCAATGACATTGTTA
TTATTGAGTGATAATTAGTAATATAATCGCTTGCTTTATTCTTTAAAAAA
AAA
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protein sequence of SMED30013079-orf-1

>SMED30013079-orf-1 ID=SMED30013079-orf-1|Name=SMED30013079-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=150bp
MNQIRIYQFMYKTGISSFSKFPSRVISTSTVANTKLGTAPTAGKIMPANT
HWTIERIVAVSMLPIYPAAWILENQFMSLLVVTTVSLHAFWGLDGVLKDY
VMERRYGPYLQPIMRNAWKVISAIGFAGFLYFEFNDIGFVKAVKKLWSI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005739mitochondrion
GO:0005743mitochondrial inner membrane
GO:0016020membrane
GO:0005740mitochondrial envelope
GO:0016021integral component of membrane
Vocabulary: biological process
TermDefinition
GO:0006099tricarboxylic acid cycle
GO:0055114oxidation-reduction process
Vocabulary: molecular function
TermDefinition
GO:0046872metal ion binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000101muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR034804SQR/QFR_C/D
IPR007992CybS
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF05328CybScoord: 39..148
e-value: 6.1E-23
score: 81.0
NoneNo IPR availableTMHMMTMhelixcoord: 52..71
NoneNo IPR availableTMHMMTMhelixcoord: 75..97
NoneNo IPR availableTMHMMTMhelixcoord: 118..140
IPR034804Fumarate reductase/succinate dehydrogenase, transmembrane subunitGENE3DG3DSA:1.20.1300.10coord: 23..149
e-value: 1.3E-33
score: 117.3
IPR007992Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, CybSPANTHERPTHR13337SUCCINATE DEHYDROGENASEcoord: 29..148