Protein kinase domain-containing protein
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30013054 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 17
Homology
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: NEK1 (NIMA related kinase 1 [Source:HGNC Symbol;Acc:HGNC:7744]) HSP 1 Score: 351.288 bits (900), Expect = 4.381e-101 Identity = 215/573 (37.52%), Postives = 319/573 (55.67%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNE-----TKKQIQKRPSTASVVSRVDSGLSNKKKARVGRDNALINSP--YMQIPNRNIHDKR---------VSP----------RIVKQDA--------------------NPLVVRQQELNNKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMT--PAAIKQKINSPAYDRYYAELDRIKEQQVL-------RENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDAS------KPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALL 1660
+ RL KIG+G+FG A LV+S D ++YV+KEI + +M KER E+R+EV++L+ M HPNIV Y SFEE G +I+M+YCEGGDL+++I Q GVLF+EDQ+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +V+LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYELC+L +AF + L ++I ++ PV +S LR+LV +F+R P +RP++N+IL K FI K+I+KFL P+ L+ E T + +P A + + +S ++ + A P Y + ++ +H+K+ +P R + ++A + + Q + K ++E IN RE GW+NVL S ++ A G T P++ + Y+ Y+A D++++Q+ RE GR L + + GA G + DA K SK R G++ A Q ++EF++RKR+A K R M +L
Sbjct: 4 YVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQ--LIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVL------SAGGSGEVKAPFLGSGGTIAPSSFSSR---GQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPG-----FPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQ--VEEFLQRKREAMQNKARAEGHMGIL 558
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: NEK1 (NIMA related kinase 1 [Source:HGNC Symbol;Acc:HGNC:7744]) HSP 1 Score: 348.977 bits (894), Expect = 1.994e-100 Identity = 215/573 (37.52%), Postives = 318/573 (55.50%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNE-----TKKQIQKRPSTASVVSRVDSGLSNKKKARVGRDNALINSP--YMQIPNRNIHDKR---------VSP----------RIVKQDA--------------------NPLVVRQQELNNKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMT--PAAIKQKINSPAYDRYYAELDRIKEQQVL-------RENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDAS------KPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALL 1660
+ RL KIG+G+FG A LV+S D ++YV+KEI + +M KER E+R+EV++L+ M HPNIV Y SFEE G +I+M+YCEGGDL+++I Q GVLF+EDQ+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +V+LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYELC+L +AF + L ++I ++ PV +S LR+LV +F+R P +RP++N+IL K FI K+I+KFL P+ L+ E T + +P A + + +S ++ + A P Y + ++ +H+K+ +P R + ++A + + Q + K ++E IN RE GW+NVL S ++ A G T P++ + Y+ Y+A D++++Q+ RE GR L + + GA G + DA K SK R G++ A Q ++EF++RKR+A K R M L
Sbjct: 4 YVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQ--LIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVL------SAGGSGEVKAPFLGSGGTIAPSSFSSR---GQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPG-----FPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQ--VEEFLQRKREAMQNKARAEGHMVYL 558
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: NEK1 (NIMA related kinase 1 [Source:HGNC Symbol;Acc:HGNC:7744]) HSP 1 Score: 347.051 bits (889), Expect = 4.160e-100 Identity = 152/266 (57.14%), Postives = 208/266 (78.20%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE 922
+ RL KIG+G+FG A LV+S D ++YV+KEI + +M KER E+R+EV++L+ M HPNIV Y SFEE G +I+M+YCEGGDL+++I Q GVLF+EDQ+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +V+LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYELC+L +AF + L ++I ++ PV +S LR+LV +F+R P +RP++N+IL K FI K+I+KFL P+
Sbjct: 4 YVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQ 269
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: NEK1 (NIMA related kinase 1 [Source:HGNC Symbol;Acc:HGNC:7744]) HSP 1 Score: 347.051 bits (889), Expect = 5.136e-100 Identity = 152/266 (57.14%), Postives = 208/266 (78.20%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE 922
+ RL KIG+G+FG A LV+S D ++YV+KEI + +M KER E+R+EV++L+ M HPNIV Y SFEE G +I+M+YCEGGDL+++I Q GVLF+EDQ+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +V+LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYELC+L +AF + L ++I ++ PV +S LR+LV +F+R P +RP++N+IL K FI K+I+KFL P+
Sbjct: 4 YVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQ 269
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: NEK1 (NIMA related kinase 1 [Source:HGNC Symbol;Acc:HGNC:7744]) HSP 1 Score: 347.436 bits (890), Expect = 6.432e-100 Identity = 152/266 (57.14%), Postives = 208/266 (78.20%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE 922
+ RL KIG+G+FG A LV+S D ++YV+KEI + +M KER E+R+EV++L+ M HPNIV Y SFEE G +I+M+YCEGGDL+++I Q GVLF+EDQ+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +V+LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYELC+L +AF + L ++I ++ PV +S LR+LV +F+R P +RP++N+IL K FI K+I+KFL P+
Sbjct: 4 YVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQ 269
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: nekl-2 (Serine/threonine-protein kinase nekl-2 [Source:UniProtKB/Swiss-Prot;Acc:O01775]) HSP 1 Score: 223.402 bits (568), Expect = 1.962e-64 Identity = 111/269 (41.26%), Postives = 166/269 (61.71%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDC--KKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGV-------LFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSV-KLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFIL 892
+ +++++ +G+GAFG+ WL K D +K ++K I + M EKE + EV+LL K+ HP I+ Y +SF I+M+Y EGG L R I Q + F E VLDYF QI +AL H+H + I+HRD+KPQNI + + +V KL DFGI+K + + +A + T +GTP Y SPEICE PYN KSD+WSLGC+LYEL L AF G+++ + M+I + P+ S ++ LV+N+ + D+RP+++ +L P +L
Sbjct: 1 MDNYEKVRVVGRGAFGVCWLCRGKNDASHQKVIIKLINTHGMTEKEENSIQSEVNLLKKVQHPLIIGYIDSFIMDNQLGIVMQYAEGGTLERLINDQRAIKDSNMREYFPEKTVLDYFTQILIALNHMHQKNIVHRDLKPQNILMNRRKTVLKLSDFGISKELGTKSAAS-TVIGTPNYLSPEICESRPYNQKSDMWSLGCVLYELLQLERAFDGENLPAIVMKITRSKQNPLGDHVSNDVKMLVENLLKTHTDKRPDVSQLLSDPLVL 268
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: nekl-1 (NEK (NEver in mitosis Kinase) Like [Source:UniProtKB/TrEMBL;Acc:A0A061AD48]) HSP 1 Score: 232.646 bits (592), Expect = 8.912e-63 Identity = 111/254 (43.70%), Postives = 163/254 (64.17%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKI-KTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTAL--TKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILR 877
++R+ +G+GAFG A L + D ++KEI +Y + +R A EVSLLS++ HPNI+ YY+SFEE G I MEY +GG L + + +TQN L E+Q+ D IQ+C A+ ++H+ +LHRD+K N+FLT+++ VK+GDFGI+K M + T KT VGTP+Y SPE+C G YN KSD+W+LGCILYE+C L AF G ++ L I Y PV +SA ++ ++ + + P +RP+ L+
Sbjct: 133 YERIRTVGKGAFGSAVLYRRREDSSLVIIKEINMYDLDSSQRRLALNEVSLLSRIEHPNIIAYYDSFEEEGVLMIEMEYADGGTLAQMLSRTQN--LLDEEQIGDMMIQMCSAVAYLHENSVLHRDLKTANVFLTRDSFVKIGDFGISKIMGTETLAQGAKTVVGTPYYISPEMCSGVSYNEKSDMWALGCILYEMCCLKKAFEGDNLPALVNSIMTCAYTPVKGPYSAEMKMVIRELLQLDPQKRPSAPQALK 384
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: nekl-1 (NEK (NEver in mitosis Kinase) Like [Source:UniProtKB/TrEMBL;Acc:A0A061AD48]) HSP 1 Score: 232.646 bits (592), Expect = 1.105e-62 Identity = 110/248 (44.35%), Postives = 161/248 (64.92%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKI-KTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTAL--TKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPN 859
++R+ +G+GAFG A L + D ++KEI +Y + +R A EVSLLS++ HPNI+ YY+SFEE G I MEY +GG L + + +TQN L E+Q+ D IQ+C A+ ++H+ +LHRD+K N+FLT+++ VK+GDFGI+K M + T KT VGTP+Y SPE+C G YN KSD+W+LGCILYE+C L AF G ++ L I Y PV +SA ++ ++ + + P +RP+
Sbjct: 171 YERIRTVGKGAFGSAVLYRRREDSSLVIIKEINMYDLDSSQRRLALNEVSLLSRIEHPNIIAYYDSFEEEGVLMIEMEYADGGTLAQMLSRTQN--LLDEEQIGDMMIQMCSAVAYLHENSVLHRDLKTANVFLTRDSFVKIGDFGISKIMGTETLAQGAKTVVGTPYYISPEMCSGVSYNEKSDMWALGCILYEMCCLKKAFEGDNLPALVNSIMTCAYTPVKGPYSAEMKMVIRELLQLDPQKRPS 416
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: nekl-3 (Serine/threonine-protein kinase nekl-3 [Source:UniProtKB/Swiss-Prot;Acc:G5EFM9]) HSP 1 Score: 185.267 bits (469), Expect = 6.958e-52 Identity = 97/252 (38.49%), Postives = 146/252 (57.94%), Query Frame = 2
Query: 140 KIGQGAFGIAWLVESKIDCKKYVLKEIGLYKM-HEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIK--TQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITI--LAMRIKKVNYKPVPS-VFSAPLRALVDNMFRRRPDERPNINTILR 877
KIG+G F + + LK+I +++M +K R + KE+ LL ++NH N++ YY SF + +I++E E GD+ R IK + G L E + YF+Q+ AL H+H +RI+HRDIKP N+F+T N VKLGD G+ + S T + VGTP+Y SPE + YN KSD+WS GC+LYE+ +L FYG + + L +I+ Y P+P+ ++S LR LV +RP + +L+
Sbjct: 28 KIGKGQFSEVFRAQCTWVDLHVALKKIQVFEMVDQKARQDCLKEIDLLKQLNHVNVIRYYASFIDNNQLNIVLELAEAGDMSRMIKHFKKGGRLIPEKTIWKYFVQLARALAHMHSKRIMHRDIKPANVFITGNGIVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIQESGYNFKSDLWSTGCLLYEMAALQSPFYGDKMNLYSLCKKIENCEYPPLPADIYSTQLRDLVSRCILPEASKRPETSEVLQ 279
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: nekl-3 (Serine/threonine-protein kinase nekl-3 [Source:UniProtKB/Swiss-Prot;Acc:G5EFM9]) HSP 1 Score: 185.267 bits (469), Expect = 6.958e-52 Identity = 97/252 (38.49%), Postives = 146/252 (57.94%), Query Frame = 2
Query: 140 KIGQGAFGIAWLVESKIDCKKYVLKEIGLYKM-HEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIK--TQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITI--LAMRIKKVNYKPVPS-VFSAPLRALVDNMFRRRPDERPNINTILR 877
KIG+G F + + LK+I +++M +K R + KE+ LL ++NH N++ YY SF + +I++E E GD+ R IK + G L E + YF+Q+ AL H+H +RI+HRDIKP N+F+T N VKLGD G+ + S T + VGTP+Y SPE + YN KSD+WS GC+LYE+ +L FYG + + L +I+ Y P+P+ ++S LR LV +RP + +L+
Sbjct: 28 KIGKGQFSEVFRAQCTWVDLHVALKKIQVFEMVDQKARQDCLKEIDLLKQLNHVNVIRYYASFIDNNQLNIVLELAEAGDMSRMIKHFKKGGRLIPEKTIWKYFVQLARALAHMHSKRIMHRDIKPANVFITGNGIVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIQESGYNFKSDLWSTGCLLYEMAALQSPFYGDKMNLYSLCKKIENCEYPPLPADIYSTQLRDLVSRCILPEASKRPETSEVLQ 279
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: niki (gene:FBgn0045980 transcript:FBtr0084737) HSP 1 Score: 215.698 bits (548), Expect = 1.524e-57 Identity = 110/267 (41.20%), Postives = 165/267 (61.80%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVL-FKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRK--PFILKKIDK 907
+++++++ +GQG+FGIA L K D + V K+I L ++ R A EV + SK++HPNIV+Y SF + I MEY +GG L + I + G L F E ++ F QI A+ ++H + ILHRD+K N+FL + VK+GDFGI+K M+ T +T +GTP+YFSPE+CEG Y+NKSDIW+LGCIL E+C L F +++ L +I NY PVPS +++ LR+L+ N+ + RP + +L P I + + K
Sbjct: 102 LANYEKVRVVGQGSFGIAILYRRKSDGHQIVFKQINLSELSPPGRDLAMNEVDVFSKLHHPNIVSYLGSFIKDNTLLIEMEYADGGTLAQIIAERQGKLHFPERYIIAVFEQISSAINYMHSENILHRDLKTANVFLNRRGIVKIGDFGISKIMN-TKIHAQTVLGTPYYFSPEMCEGKEYDNKSDIWALGCILGEMCCLKKTFAASNLSELVTKIMAGNYTPVPSGYTSGLRSLMSNLLQVEAPRRPTASEVLVYWIPLIFRSLGK 367
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: Nek2 (gene:FBgn0029970 transcript:FBtr0071142) HSP 1 Score: 182.185 bits (461), Expect = 4.352e-47 Identity = 96/296 (32.43%), Postives = 165/296 (55.74%), Query Frame = 2
Query: 113 SISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSF--EEVGCFHILMEYCEGGDLYRKIKTQNGVL--FKEDQVLDYFIQICLALKHIHDQ----RILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNETKKQIQKRPSTA 976
++ D++ L +G G+FG + V K + + K + ++ E + E+S+L ++ HPNIV YY+ E +I+ME C GGDL + ++ F+E + Q+C AL+ H++ ILHRDIKP NIFL + KLGDFG+A+ + + + VGTP Y SPE+ +G Y+ KSD+W++GC++YE+C+L F G+ L+ +I + + +P+++S L+ ++ M ++RP I I+R P +++ I + L + P+L ++ + PS A
Sbjct: 15 TLQDYEVLAVMGNGSFGTCYKVRDKSTGELFAWKGMNYDELDEAKCDALVSEISVLRQLQHPNIVQYYHHLVNREAKSVYIVMECCAGGDLAQIVQRARSQRQRFEEPYIWRVLFQLCRALQVCHNKIPNGTILHRDIKPANIFLDAAGNAKLGDFGLARMLRRDQSFAASFVGTPHYMSPELVKGRKYDRKSDVWAVGCLVYEMCALRPPFRGRAFDQLSEKIAQGEFSRIPAIYSTDLQEIIAFMLAVDHEQRPGIEVIIRHPLVVRNISE-LDGKFPILVDSGEDFYTLPSGA 309
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: fu (gene:FBgn0001079 transcript:FBtr0074602) HSP 1 Score: 141.354 bits (355), Expect = 9.285e-34 Identity = 94/313 (30.03%), Postives = 152/313 (48.56%), Query Frame = 2
Query: 143 IGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKED--QVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKI-----DKFLPPENPLLN---ETKKQIQKRPSTASV---VSRVDSGLSNKKKARVGRDN 1042
+GQG+FG + K D K +K I KE R+E + +++ HP+++ SFE ++ E+ DL+R + + NG + +E +V + + AL ++H RILHRD+KPQN+ L KN KL DFG+A+ M T + + GTP Y +PE+ PY++ +D+WSLGCI YE + F I L IK + K PS ++ R+ + + + P R + +L PF+ +I E+P N + K Q P + ++ +D G S ++ RD+
Sbjct: 10 VGQGSFGCVYKATRKDDSKVVAIKVISKRGRATKELKNLRRECDIQARLKHPHVIEMIESFESKTDLFVVTEFALM-DLHRYL-SYNGAMGEEPARRVTGHLVS---ALYYLHSNRILHRDLKPQNVLLDKNMHAKLCDFGLARNMTLGTHVLTSIKGTPLYMAPELLAEQPYDHHADMWSLGCIAYESMAGQPPFCASSILHLVKMIKHEDVK-WPSTLTSECRSFLQGLLEKDPGLRISWTQLLCHPFVEGRIFIAETQAEAAKESPFTNPEAKVKSSKQSDPEVGDLDEALAALDFGESRQENLTTSRDS 316
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: polo (gene:FBgn0003124 transcript:FBtr0074839) HSP 1 Score: 136.346 bits (342), Expect = 6.932e-33 Identity = 87/269 (32.34%), Postives = 144/269 (53.53%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAW-LVESKID---CKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFIL-KKIDKFLP 916
+KR+ G+G F + +++ + D K V K++ + K ++KE+ +E+++ +NHPNIV ++N FE+ +I++E C+ + K + + E + Y QI +K++HD RI+HRD+K N+FL VK+GDFG+A ++ KT GTP Y +PEI ++ + DIWS+GC++Y L F + + +IKK Y+ VPS P +V M + P+ RP I +L F+ K+ FLP
Sbjct: 25 YKRMRFFGKGGFAKCYEIIDVETDDVFAGKIVSKKL-MIKHNQKEK--TAQEITIHRSLNHPNIVKFHNYFEDSQNIYIVLELCKKRSMMELHKRRKSI--TEFECRYYIYQIIQGVKYLHDNRIIHRDLKLGNLFLNDLLHVKIGDFGLATRIEYEGERKKTLCGTPNYIAPEILTKKGHSFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKIKKCEYR-VPSYLRKPAADMVIAMLQPNPESRPAIGQLLNFEFLKGSKVPMFLP 287
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: polo (gene:FBgn0003124 transcript:FBtr0100318) HSP 1 Score: 136.346 bits (342), Expect = 6.932e-33 Identity = 87/269 (32.34%), Postives = 144/269 (53.53%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAW-LVESKID---CKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFIL-KKIDKFLP 916
+KR+ G+G F + +++ + D K V K++ + K ++KE+ +E+++ +NHPNIV ++N FE+ +I++E C+ + K + + E + Y QI +K++HD RI+HRD+K N+FL VK+GDFG+A ++ KT GTP Y +PEI ++ + DIWS+GC++Y L F + + +IKK Y+ VPS P +V M + P+ RP I +L F+ K+ FLP
Sbjct: 25 YKRMRFFGKGGFAKCYEIIDVETDDVFAGKIVSKKL-MIKHNQKEK--TAQEITIHRSLNHPNIVKFHNYFEDSQNIYIVLELCKKRSMMELHKRRKSI--TEFECRYYIYQIIQGVKYLHDNRIIHRDLKLGNLFLNDLLHVKIGDFGLATRIEYEGERKKTLCGTPNYIAPEILTKKGHSFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKIKKCEYR-VPSYLRKPAADMVIAMLQPNPESRPAIGQLLNFEFLKGSKVPMFLP 287
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: nek1 (NIMA-related kinase 1 [Source:ZFIN;Acc:ZDB-GENE-040730-1]) HSP 1 Score: 347.436 bits (890), Expect = 2.931e-110 Identity = 154/275 (56.00%), Postives = 216/275 (78.55%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNETKK 949
++RL KIG+G+FG A LV+S+ D ++YV+KEIG+ +M KER E+RKEV++L+ M+HPNIV Y SFEE GC +I+M+YCEGGDL++KI Q G LF E+Q+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +V+LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYE+C+L +AF ++ L ++I + +Y PV +S LR+L+ +F+R P ERP+++TIL KPF+ ++I KFL P+ +E +
Sbjct: 4 YERLKKIGEGSFGKAILVKSRTDGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKPFLARRIHKFLSPQVTYTHEQTR 278
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: CABZ01040556.1 (NIMA related kinase 3 [Source:NCBI gene;Acc:100536894]) HSP 1 Score: 270.011 bits (689), Expect = 1.004e-79 Identity = 130/274 (47.45%), Postives = 187/274 (68.25%), Query Frame = 2
Query: 143 IGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNETKKQIQKR 964
IG+G+FG A LV+ + K YV+KEI L K E ++R E LLSKM HPNIV + +SFE G +I+ME+C GGDL ++I+ QN +LF E+Q+L +F Q+CL KHIHD+R+LHRD+K +NIFLT + +VK+GDFG A +++S A +T VGTP+Y SPEI + PYNNKSD+WSLGC+LYELC+L + F + L +++ + +Y P+PS F L L+ +MF+ P +RP+I+TIL + + + K L EN L + + + +R
Sbjct: 10 IGEGSFGRALLVQPIHEEKHYVMKEIRLPKA-ESGMKKSRSEAVLLSKMKHPNIVAFRDSFEADGHLYIVMEFCSGGDLMQRIRQQNRLLFSEEQILKWFAQMCLGTKHIHDKRVLHRDLKSKNIFLTDSGTVKVGDFGSACSLNSAKAYAQTYVGTPYYVSPEIWDNKPYNNKSDVWSLGCVLYELCTLQHPFQARSWKSLILQVCRGSYAPLPSHFPYELHYLIKHMFKTNPKDRPSIHTILSSHRVSRLLHKHLTLENQELLQERTRHWRR 282
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: nek4 (NIMA-related kinase 4 [Source:ZFIN;Acc:ZDB-GENE-040426-1392]) HSP 1 Score: 250.366 bits (638), Expect = 4.733e-69 Identity = 113/258 (43.80%), Postives = 173/258 (67.05%), Query Frame = 2
Query: 143 IGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGC-FHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFL 913
+G+G++G LV K D K+YV+K++ L +ER A +E LLS++ HPNIV Y S+E C +I+M +CEGGDLY ++K Q G L E QV+++F+QI +AL+++H++ ILHRD+K QNIFLTK N +K+GD GIA+ +++ + T +GTP+Y SPE+ PYN KSD+W+LGC +YE+ +L +AF +D+ L RI + +PS + L L+ M ++P++RP++ ILR+P+I +I FL
Sbjct: 10 VGKGSYGEVNLVRHKSDRKQYVIKKLNLRTSSRRERRAAEQEAQLLSQLKHPNIVMYRESWEGEDCQLYIVMGFCEGGDLYHRLKQQKGELLPERQVVEWFVQIAMALQYLHEKHILHRDLKTQNIFLTKTNIIKVGDLGIARVLENQNDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIVEGKLPQMPSKYDPQLGELIKRMLCKKPEDRPDVKHILRQPYIKHQISMFL 267
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: nek4 (NIMA-related kinase 4 [Source:ZFIN;Acc:ZDB-GENE-040426-1392]) HSP 1 Score: 250.366 bits (638), Expect = 5.500e-69 Identity = 113/258 (43.80%), Postives = 173/258 (67.05%), Query Frame = 2
Query: 143 IGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGC-FHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFL 913
+G+G++G LV K D K+YV+K++ L +ER A +E LLS++ HPNIV Y S+E C +I+M +CEGGDLY ++K Q G L E QV+++F+QI +AL+++H++ ILHRD+K QNIFLTK N +K+GD GIA+ +++ + T +GTP+Y SPE+ PYN KSD+W+LGC +YE+ +L +AF +D+ L RI + +PS + L L+ M ++P++RP++ ILR+P+I +I FL
Sbjct: 10 VGKGSYGEVNLVRHKSDRKQYVIKKLNLRTSSRRERRAAEQEAQLLSQLKHPNIVMYRESWEGEDCQLYIVMGFCEGGDLYHRLKQQKGELLPERQVVEWFVQIAMALQYLHEKHILHRDLKTQNIFLTKTNIIKVGDLGIARVLENQNDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIVEGKLPQMPSKYDPQLGELIKRMLCKKPEDRPDVKHILRQPYIKHQISMFL 267
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: nek8 (NIMA-related kinase 8 [Source:NCBI gene;Acc:171094]) HSP 1 Score: 223.402 bits (568), Expect = 6.715e-61 Identity = 122/312 (39.10%), Postives = 175/312 (56.09%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNS-VKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKI---------DKFLPPENPLLNETKKQIQKRPSTASVVSRVDSGLSNKKKARV 1030
++++ +G+GAFGI L + D +LKEI + +M ER A+ E +L ++HPNI+ YY +F E I MEY GG L I+ + L ED +L +F+QI LAL H+H++ ILHRD+K QNI L K+ VK+GDFGI+K + S + T VGTP Y SPE+CEG PYN KSDIW+LGC+LYEL SL AF ++ L ++I + P+ +S LR L+ NM P +RP +N I+ ++ + K E PL N + RP +R GLS+ +++
Sbjct: 4 YEKIKVVGRGAFGIVHLCRRRTDSALVILKEIPVEQMTRDERLAAQNECQVLKLLSHPNIIEYYENFLEDKALMIAMEYAPGGTLADYIQKRCNSLLDEDTILHFFVQILLALYHVHNKLILHRDLKTQNILLDKHQMIVKIGDFGISKILVSKSK-AYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLILNMLNLDPSKRPQLNEIMAHAICIRPLLNLYTDIGNVKMRRIEKPLSN-VQAGPHGRPGGWITSTRTRGGLSSLTSSKM 313
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: nek1 (NIMA-related kinase 1 [Source:NCBI gene;Acc:100036653]) HSP 1 Score: 382.489 bits (981), Expect = 4.380e-112 Identity = 238/650 (36.62%), Postives = 367/650 (56.46%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPEN--------PLLNETKKQIQ----KRPSTASVV-------------SRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHDKRVSPRIVKQDA------NPLVVRQQELNNKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPA-AIKQKINSPAYDRYYAELDRIKEQQVLRENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKI----------DASKPYDE----RSKMNELARIHGKIGGN------AADQA-VIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQ--SPIPRQTPRSIEEETTERLNKIRQKNFEIRQGMGFE-DFVKNSDIDGNGNFENFNQLEARKALEDLKKRV 1897
++ + R++KIG+G+FG A LV+SK D K+YV+KEIG+ KM KER E+R+EV++L+ M HPNIV Y SFEE GC +I+M+YCEGGDL+++I +Q GVLF EDQ+LD+F+Q+CLALKH+HD++ILHRDIK QNIFLTK +++LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYE+C+L +AF ++ L ++I + +Y PV +S LR L+ +F+R P +RP++N+IL KPFI ++I+KFL P++ +N K IQ KRP+ A VV ++ L+ KK + + + P ++ KR+ R ++++ N L Q K ++E IN RE GW+NV+SS S + K +G A Q + Y+ Y++ D++++Q +EN A+ + + + D ++PD Y ++ A P+ + R ++ L +H + GN +A++A ++EF++RK +A K R M + C + S + E+E RL +IR +NF RQ + + K + D +G + + RK +E LK +
Sbjct: 1 MNKYVRVSKIGEGSFGKAILVKSKDDGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFLSPQHIAEEFGLGAFVNHPKPGIQAAAAKRPTPAQVVPASPPAHKITKPAAKYGVPLTTKKSSDAAKKHH-DKKPQIRHRQDGAKKKRMDVRQLEKEKRQREQFNLLRAEQMRRLEKERLERINRAREQGWRNVISSGGSG--------DIKAPYFGGGGGVAPPQGMGRGPYEHYHSIFDQMQQQ---KENIKAAVEKEVKLKADIQNPD----------TYSRVPKHPEQENCPSAHSPFPDGDAVRRELKRLQEVHNQAIGNKIQGHVSAEKARQVEEFLQRKHEAMQNKARAEGHMDAVHNLAAICGGRPNSAGGGKARNREEKEYLARLRQIRLQNFNERQQIKAKLRGAKGDEGDADGKESSEDAELRRKKIEALKAQA 628
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: nek1 (NIMA-related kinase 1 [Source:NCBI gene;Acc:100036653]) HSP 1 Score: 371.318 bits (952), Expect = 4.526e-108 Identity = 232/622 (37.30%), Postives = 348/622 (55.95%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPEN--------PLLNETKKQIQ----KRPSTASVVSRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHDK------RVSPRIVKQDANP----------------LVVRQQELN------------------NKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPA-AIKQKINSPAYDRYYAELDRIKEQQVLRENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDASKPYDE----RSKMNELARIHGKIGGN------AADQA-VIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQ--SPIPRQTPRSIEEETTERLNKIRQKNFEIRQ 1783
++ + R++KIG+G+FG A LV+SK D K+YV+KEIG+ KM KER E+R+EV++L+ M HPNIV Y SFEE GC +I+M+YCEGGDL+++I +Q GVLF EDQ+LD+F+Q+CLALKH+HD++ILHRDIK QNIFLTK +++LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYE+C+L +AF ++ L ++I + +Y PV +S LR L+ +F+R P +RP++N+IL KPFI ++I+KFL P++ +N K IQ KRP+ A VV S ++K + + + + HDK R +P + + NP + VRQ E K ++E IN RE GW+NV+SS S + K +G A Q + Y+ Y++ D++++Q +EN A+ + + + D ++PD A P+ + R ++ L +H + GN +A++A ++EF++RK +A K R M + C + S + E+E RL +IR +NF RQ
Sbjct: 1 MNKYVRVSKIGEGSFGKAILVKSKDDGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFLSPQHIAEEFGLGAFVNHPKPGIQAAAAKRPTPAQVVPA--SPPAHKITKPAAKYGVPLTTKKSSDAAKKHHDKKPQIRHRQAPAVPLKKVNPVDERRKMFEDGAKKKRMDVRQLEKEKRQREQFNLLRAEQMRRLEKERLERINRAREQGWRNVISSGGSG--------DIKAPYFGGGGGVAPPQGMGRGPYEHYHSIFDQMQQQ---KENIKAAVEKEVKLKADIQNPDTYSRVPKHPEQENCPSAHSPFPDGDAVRRELKRLQEVHNQAIGNKIQGHVSAEKARQVEEFLQRKHEAMQNKARAEGHMDAVHNLAAICGGRPNSAGGGKARNREEKEYLARLRQIRLQNFNERQ 609
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: nek1 (NIMA-related kinase 1 [Source:NCBI gene;Acc:100036653]) HSP 1 Score: 368.237 bits (944), Expect = 4.058e-107 Identity = 221/579 (38.17%), Postives = 331/579 (57.17%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPEN--------PLLNETKKQIQ----KRPSTASVVSRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHDK------RVSPRIVKQDANP----------------LVVRQQELN------------------NKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPA-AIKQKINSPAYDRYYAELDRIKEQQVLRENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDASKPYDE----RSKMNELARIHGKIGGN------AADQA-VIQEFIERKRQAKNIKNRNGSGMALL 1660
++ + R++KIG+G+FG A LV+SK D K+YV+KEIG+ KM KER E+R+EV++L+ M HPNIV Y SFEE GC +I+M+YCEGGDL+++I +Q GVLF EDQ+LD+F+Q+CLALKH+HD++ILHRDIK QNIFLTK +++LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYE+C+L +AF ++ L ++I + +Y PV +S LR L+ +F+R P +RP++N+IL KPFI ++I+KFL P++ +N K IQ KRP+ A VV S ++K + + + + HDK R +P + + NP + VRQ E K ++E IN RE GW+NV+SS S + K +G A Q + Y+ Y++ D++++Q +EN A+ + + + D ++PD A P+ + R ++ L +H + GN +A++A ++EF++RK +A K R M L
Sbjct: 1 MNKYVRVSKIGEGSFGKAILVKSKDDGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFLSPQHIAEEFGLGAFVNHPKPGIQAAAAKRPTPAQVVPA--SPPAHKITKPAAKYGVPLTTKKSSDAAKKHHDKKPQIRHRQAPAVPLKKVNPVDERRKMFEDGAKKKRMDVRQLEKEKRQREQFNLLRAEQMRRLEKERLERINRAREQGWRNVISSGGSG--------DIKAPYFGGGGGVAPPQGMGRGPYEHYHSIFDQMQQQ---KENIKAAVEKEVKLKADIQNPDTYSRVPKHPEQENCPSAHSPFPDGDAVRRELKRLQEVHNQAIGNKIQGHVSAEKARQVEEFLQRKHEAMQNKARAEGHMEYL 566
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: nek1 (NIMA-related kinase 1 [Source:NCBI gene;Acc:100036653]) HSP 1 Score: 368.237 bits (944), Expect = 4.234e-107 Identity = 221/576 (38.37%), Postives = 329/576 (57.12%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPEN--------PLLNETKKQIQ----KRPSTASVVSRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHDK------RVSPRIVKQDANP----------------LVVRQQELN------------------NKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPA-AIKQKINSPAYDRYYAELDRIKEQQVLRENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDASKPYDE----RSKMNELARIHGKIGGN------AADQA-VIQEFIERKRQAKNIKNRNGSGMALL 1660
+ R++KIG+G+FG A LV+SK D K+YV+KEIG+ KM KER E+R+EV++L+ M HPNIV Y SFEE GC +I+M+YCEGGDL+++I +Q GVLF EDQ+LD+F+Q+CLALKH+HD++ILHRDIK QNIFLTK +++LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYE+C+L +AF ++ L ++I + +Y PV +S LR L+ +F+R P +RP++N+IL KPFI ++I+KFL P++ +N K IQ KRP+ A VV S ++K + + + + HDK R +P + + NP + VRQ E K ++E IN RE GW+NV+SS S + K +G A Q + Y+ Y++ D++++Q +EN A+ + + + D ++PD A P+ + R ++ L +H + GN +A++A ++EF++RK +A K R M L
Sbjct: 4 YVRVSKIGEGSFGKAILVKSKDDGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFLSPQHIAEEFGLGAFVNHPKPGIQAAAAKRPTPAQVVPA--SPPAHKITKPAAKYGVPLTTKKSSDAAKKHHDKKPQIRHRQAPAVPLKKVNPVDERRKMFEDGAKKKRMDVRQLEKEKRQREQFNLLRAEQMRRLEKERLERINRAREQGWRNVISSGGSG--------DIKAPYFGGGGGVAPPQGMGRGPYEHYHSIFDQMQQQ---KENIKAAVEKEVKLKADIQNPDTYSRVPKHPEQENCPSAHSPFPDGDAVRRELKRLQEVHNQAIGNKIQGHVSAEKARQVEEFLQRKHEAMQNKARAEGHMEYL 566
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: sez6l2 (seizure related 6 homolog like 2 [Source:Xenbase;Acc:XB-GENE-921456]) HSP 1 Score: 305.834 bits (782), Expect = 2.320e-94 Identity = 142/261 (54.41%), Postives = 196/261 (75.10%), Query Frame = 2
Query: 143 IGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSV-KLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE 922
IG+GAFG A+L + K D + V+KEI L KM KE+ + KEV LL+KM HPNIVT+++S EE +I+MEYC+GGDL +++ Q GVLF+EDQ+L +F+QI L LKHIHD+++LHRDIK QNIFL+ N ++ KLGDFGIA+ +++T L +T VGTP+Y SPEICE PYNNK+DIWSLGC+LYELC+L + F + L ++I + Y+P+P+ +S LR LV +F+ +RP+IN+IL+KPF+ K+I+K L PE
Sbjct: 10 IGEGAFGKAYLAKGKSDNMQCVIKEINLSKMPTKEKEASHKEVVLLAKMKHPNIVTFFSSIEERNKLYIVMEYCDGGDLMKRVNKQRGVLFEEDQILSWFVQISLGLKHIHDRKVLHRDIKAQNIFLSNNGTLAKLGDFGIARMLNNTMELARTCVGTPYYLSPEICENKPYNNKTDIWSLGCVLYELCALKHPFEASSLRQLVLKICRGRYEPIPTKYSYDLRILVSQLFKISSRDRPSINSILKKPFLEKRINKHLSPE 270
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Nek1 (NIMA (never in mitosis gene a)-related expressed kinase 1 [Source:MGI Symbol;Acc:MGI:97303]) HSP 1 Score: 369.392 bits (947), Expect = 1.224e-107 Identity = 244/652 (37.42%), Postives = 354/652 (54.29%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPP-----ENPLLNETKKQIQ----KRPSTASVVSR-VDSGLSNKKKARVG--------RDNALI-------NSPYMQIPNRNIHDKRVSPRIVKQDANP------------------LVVRQQELNNKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPAAIKQKINSP--AYDRYYAELDRIKEQQVLRENEGRALMQHMLKQN---DNKSPDGKLLKVG----ADGLYRKIDAS------KPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALLMG-GHQFCAEQSPIPRQTPRSIEEET-TERLNKIRQKNFEIRQGMGFEDFVKNSDIDGNGNFENFNQLEAR-KALEDLKKRV 1897
+ RL KIG+G+FG A LV+S D + YV+KEI + +M +KER E+R+EV++L+ M HPNIV Y SFEE G +I+M+YCEGGDL+++I Q G LF+EDQ+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +V+LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYELC+L +AF ++ L ++I ++ PV +S LR+L+ +F+R P +RP++N+IL K FI K+I+KFL P E L +K Q KRP++ VS V + K A+ G D L+ + QIP + ++ ++ ++ A L Q + K ++E IN RE GW+NVL + S K + +G+ A+ SP Y+ Y+A D++ Q LR + A + + + P G ++ G A G Y DA K SK R G + A+Q ++EF++RKR+A K R + LL + S PR+ EEE RL +IR +NF RQ + + +N + DG E + + R K +E LK +
Sbjct: 4 YVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSG--------EVKASFFGIG-GAVSPSPCSPRGQYEHYHAIFDQM---QRLRAEDNEARWKGGIYGRWLPERGVPPG--VRPGFPSEASGHYHSPDAGAIRKTLKRLKAVSKQASTNRQKGHLAVERANQ--VEEFLQRKREAMQNKARAEGHVGLLQNLASLYGGRPSSSRGGKPRNNEEEVYLARLRQIRLQNFNERQQIKAKLRGENKEADGTKGQEATEETDMRLKKMESLKAQT 639
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Nek1 (NIMA (never in mitosis gene a)-related expressed kinase 1 [Source:MGI Symbol;Acc:MGI:97303]) HSP 1 Score: 346.665 bits (888), Expect = 4.326e-100 Identity = 198/474 (41.77%), Postives = 285/474 (60.13%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNE-------TKKQIQ----KRPSTASVVSR-VDSGLSNKKKARVG--------RDNALI-------NSPYMQIPNRNIHDKRVSPRIVKQDANP------------------LVVRQQELNNKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPAAIKQKINSP--AYDRYYAELDRIKEQQVLR--ENEGR 1399
+ RL KIG+G+FG A LV+S D + YV+KEI + +M +KER E+R+EV++L+ M HPNIV Y SFEE G +I+M+YCEGGDL+++I Q G LF+EDQ+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +V+LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYELC+L +AF ++ L ++I ++ PV +S LR+L+ +F+R P +RP++N+IL K FI K+I+KFL P+ L+ E +K Q KRP++ VS V + K A+ G D L+ + QIP + ++ ++ ++ A L Q + K ++E IN RE GW+NVL + S K + +G+ A+ SP Y+ Y+A D++ Q LR +NE R
Sbjct: 4 YVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQ--LIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSG--------EVKASFFGIG-GAVSPSPCSPRGQYEHYHAIFDQM---QRLRAEDNEAR 463
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Nek1 (NIMA (never in mitosis gene a)-related expressed kinase 1 [Source:MGI Symbol;Acc:MGI:97303]) HSP 1 Score: 347.051 bits (889), Expect = 4.400e-100 Identity = 198/474 (41.77%), Postives = 285/474 (60.13%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNE-------TKKQIQ----KRPSTASVVSR-VDSGLSNKKKARVG--------RDNALI-------NSPYMQIPNRNIHDKRVSPRIVKQDANP------------------LVVRQQELNNKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPAAIKQKINSP--AYDRYYAELDRIKEQQVLR--ENEGR 1399
+ RL KIG+G+FG A LV+S D + YV+KEI + +M +KER E+R+EV++L+ M HPNIV Y SFEE G +I+M+YCEGGDL+++I Q G LF+EDQ+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +V+LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYELC+L +AF ++ L ++I ++ PV +S LR+L+ +F+R P +RP++N+IL K FI K+I+KFL P+ L+ E +K Q KRP++ VS V + K A+ G D L+ + QIP + ++ ++ ++ A L Q + K ++E IN RE GW+NVL + S K + +G+ A+ SP Y+ Y+A D++ Q LR +NE R
Sbjct: 4 YVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQ--LIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSG--------EVKASFFGIG-GAVSPSPCSPRGQYEHYHAIFDQM---QRLRAEDNEAR 463
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Nek1 (NIMA (never in mitosis gene a)-related expressed kinase 1 [Source:MGI Symbol;Acc:MGI:97303]) HSP 1 Score: 343.584 bits (880), Expect = 3.362e-99 Identity = 150/266 (56.39%), Postives = 208/266 (78.20%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE 922
+ RL KIG+G+FG A LV+S D + YV+KEI + +M +KER E+R+EV++L+ M HPNIV Y SFEE G +I+M+YCEGGDL+++I Q G LF+EDQ+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +V+LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYELC+L +AF ++ L ++I ++ PV +S LR+L+ +F+R P +RP++N+IL K FI K+I+KFL P+
Sbjct: 4 YVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQ 269
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Nek5 (NIMA (never in mitosis gene a)-related expressed kinase 5 [Source:MGI Symbol;Acc:MGI:2142824]) HSP 1 Score: 277.33 bits (708), Expect = 4.446e-80 Identity = 129/270 (47.78%), Postives = 187/270 (69.26%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSV-KLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE 922
+ +F + IG+G FG +L + K + V+KEI L K E+ ++ EV LL++M HPNIVT+++SF+E G I+MEYC+GGDL ++I+ Q GV+F EDQ+L +F+QI L LKHIHD++ILHRDIK QNIFL+KN V KLGDFG A+ ++ + L +T GTP+Y SPEIC+ PYNNK+DIWSLGC+LYELC+L + F + L ++I + P+ FS L++L+ +FR P +RP++ ++L++PF+ I + L PE
Sbjct: 1 MDNFHLIKIIGEGTFGKVYLAKDKSESSHCVIKEISLTK----EKEASKNEVILLARMEHPNIVTFFSSFQENGRLFIVMEYCDGGDLMQRIQRQRGVMFSEDQILCWFVQISLGLKHIHDRKILHRDIKSQNIFLSKNGMVAKLGDFGTARTLNDSMELAQTCAGTPYYLSPEICQNRPYNNKTDIWSLGCVLYELCTLKHPFESNNFHHLVLKICQGRVAPISPHFSRDLQSLIPQLFRVSPQDRPSVTSLLKRPFLETLIARSLYPE 266
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|Q96PY6|NEK1_HUMAN (Serine/threonine-protein kinase Nek1 OS=Homo sapiens OX=9606 GN=NEK1 PE=1 SV=2) HSP 1 Score: 348.977 bits (894), Expect = 9.576e-100 Identity = 215/573 (37.52%), Postives = 318/573 (55.50%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNE-----TKKQIQKRPSTASVVSRVDSGLSNKKKARVGRDNALINSP--YMQIPNRNIHDKR---------VSP----------RIVKQDA--------------------NPLVVRQQELNNKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMT--PAAIKQKINSPAYDRYYAELDRIKEQQVL-------RENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDAS------KPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALL 1660
+ RL KIG+G+FG A LV+S D ++YV+KEI + +M KER E+R+EV++L+ M HPNIV Y SFEE G +I+M+YCEGGDL+++I Q GVLF+EDQ+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +V+LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYELC+L +AF + L ++I ++ PV +S LR+LV +F+R P +RP++N+IL K FI K+I+KFL P+ L+ E T + +P A + + +S ++ + A P Y + ++ +H+K+ +P R + ++A + + Q + K ++E IN RE GW+NVL S ++ A G T P++ + Y+ Y+A D++++Q+ RE GR L + + GA G + DA K SK R G++ A Q ++EF++RKR+A K R M L
Sbjct: 4 YVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQ--LIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVL------SAGGSGEVKAPFLGSGGTIAPSSFSSR---GQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPG-----FPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQ--VEEFLQRKREAMQNKARAEGHMVYL 558
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|P51954|NEK1_MOUSE (Serine/threonine-protein kinase Nek1 OS=Mus musculus OX=10090 GN=Nek1 PE=1 SV=2) HSP 1 Score: 346.665 bits (888), Expect = 3.026e-99 Identity = 198/474 (41.77%), Postives = 285/474 (60.13%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNE-------TKKQIQ----KRPSTASVVSR-VDSGLSNKKKARVG--------RDNALI-------NSPYMQIPNRNIHDKRVSPRIVKQDANP------------------LVVRQQELNNKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPAAIKQKINSP--AYDRYYAELDRIKEQQVLR--ENEGR 1399
+ RL KIG+G+FG A LV+S D + YV+KEI + +M +KER E+R+EV++L+ M HPNIV Y SFEE G +I+M+YCEGGDL+++I Q G LF+EDQ+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +V+LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYELC+L +AF ++ L ++I ++ PV +S LR+L+ +F+R P +RP++N+IL K FI K+I+KFL P+ L+ E +K Q KRP++ VS V + K A+ G D L+ + QIP + ++ ++ ++ A L Q + K ++E IN RE GW+NVL + S K + +G+ A+ SP Y+ Y+A D++ Q LR +NE R
Sbjct: 4 YVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESFEENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQ--LIAEEFCLKTLSKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMNSGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSG--------EVKASFFGIG-GAVSPSPCSPRGQYEHYHAIFDQM---QRLRAEDNEAR 463
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6P3R8|NEK5_HUMAN (Serine/threonine-protein kinase Nek5 OS=Homo sapiens OX=9606 GN=NEK5 PE=2 SV=1) HSP 1 Score: 306.605 bits (784), Expect = 6.476e-89 Identity = 141/264 (53.41%), Postives = 194/264 (73.48%), Query Frame = 2
Query: 134 LNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSV-KLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE 922
+ IGQGAFG A+L + K D K V+KEI KM +E+ ++KEV LL KM HPNIV ++NSF+E G I+MEYC+GGDL ++I Q GVLF EDQ+L +F+QI L LKHIHD++ILHRDIK QNIFL+KN V KLGDFGIA+ ++++ L +T +GTP+Y SPEIC+ PYNNK+DIWSLGC+LYELC+L + F G ++ L ++I + ++ P+ FS L +L+ +F+ P +RP+IN+IL++PF+ I K+L PE
Sbjct: 7 IKAIGQGAFGKAYLAKGKSDSKHCVIKEINFEKMPIQEKEASKKEVILLEKMKHPNIVAFFNSFQENGRLFIVMEYCDGGDLMKRINRQRGVLFSEDQILGWFVQISLGLKHIHDRKILHRDIKAQNIFLSKNGMVAKLGDFGIARVLNNSMELARTCIGTPYYLSPEICQNKPYNNKTDIWSLGCVLYELCTLKHPFEGNNLQQLVLKICQAHFAPISPGFSRELHSLISQLFQVSPRDRPSINSILKRPFLENLIPKYLTPE 270
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|P51956|NEK3_HUMAN (Serine/threonine-protein kinase Nek3 OS=Homo sapiens OX=9606 GN=NEK3 PE=1 SV=2) HSP 1 Score: 275.789 bits (704), Expect = 4.174e-80 Identity = 139/306 (45.42%), Postives = 189/306 (61.76%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE------NPLLNETKKQIQKRPSTASVVSRVDSGLSNK 1015
+ D+ L IG+G+FG A LV+ + + + +KEI L K + +RKE LL+KM HPNIV + SFE G +I+MEYC+GGDL +KIK Q G LF ED +L++F Q+CL + HIH +R+LHRDIK +NIFLT+N VKLGDFG A+ + + A T VGTP+Y PEI E PYNNKSDIWSLGCILYELC+L + F L +++ + P+PS +S L+ LV MF+R P RP+ T+L + + + + K LPPE +L E K P + SR+ L N+
Sbjct: 1 MDDYMVLRMIGEGSFGRALLVQHESSNQMFAMKEIRLPKSFSNTQ-NSRKEAVLLAKMKHPNIVAFKESFEAEGHLYIVMEYCDGGDLMQKIKQQKGKLFPEDMILNWFTQMCLGVNHIHKKRVLHRDIKSKNIFLTQNGKVKLGDFGSARLLSNPMAFACTYVGTPYYVPPEIWENLPYNNKSDIWSLGCILYELCTLKHPFQANSWKNLILKVCQGCISPLPSHYSYELQFLVKQMFKRNPSHRPSATTLLSRGIVARLVQKCLPPEIIMEYGEEVLEEIKNSKHNTPRKKTNPSRIRIALGNE 305
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|Q7TSC3|NEK5_MOUSE (Serine/threonine-protein kinase Nek5 OS=Mus musculus OX=10090 GN=Nek5 PE=2 SV=1) HSP 1 Score: 277.33 bits (708), Expect = 3.110e-79 Identity = 129/270 (47.78%), Postives = 187/270 (69.26%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSV-KLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE 922
+ +F + IG+G FG +L + K + V+KEI L K E+ ++ EV LL++M HPNIVT+++SF+E G I+MEYC+GGDL ++I+ Q GV+F EDQ+L +F+QI L LKHIHD++ILHRDIK QNIFL+KN V KLGDFG A+ ++ + L +T GTP+Y SPEIC+ PYNNK+DIWSLGC+LYELC+L + F + L ++I + P+ FS L++L+ +FR P +RP++ ++L++PF+ I + L PE
Sbjct: 1 MDNFHLIKIIGEGTFGKVYLAKDKSESSHCVIKEISLTK----EKEASKNEVILLARMEHPNIVTFFSSFQENGRLFIVMEYCDGGDLMQRIQRQRGVMFSEDQILCWFVQISLGLKHIHDRKILHRDIKSQNIFLSKNGMVAKLGDFGTARTLNDSMELAQTCAGTPYYLSPEICQNRPYNNKTDIWSLGCVLYELCTLKHPFESNNFHHLVLKICQGRVAPISPHFSRDLQSLIPQLFRVSPQDRPSVTSLLKRPFLETLIARSLYPE 266
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: A0A2D4I327 (Protein kinase domain-containing protein (Fragment) OS=Micrurus lemniscatus lemniscatus OX=129467 PE=4 SV=1) HSP 1 Score: 383.259 bits (983), Expect = 1.383e-115 Identity = 225/590 (38.14%), Postives = 338/590 (57.29%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNETKKQIQ---------KRPSTASVVSRVDSGLS-NKKKARVG--------RDNA--LINSPYMQIPNRNIHDKRVSPRIVKQDANPLVVRQQELNNKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPAAIKQKI-NSPAYDRYYAELDRIKEQQVLRENEGRALM---QHMLKQNDNKSPDGKLLKV--GADGLYRKIDAS------KPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQ--SPIPRQTPRSIEEETTERLNKIRQKNFEIRQ 1783
+ + ++ KIG+G+FG A LV++K + K+YV+KEI + KM KER E+R+EV++L+ M HPNIV Y SFEE GC +I+M+YCEGGDL++KI Q GV F EDQ++D+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +++LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSD+W+LGC+LYE+C+L +AF ++ L ++I ++ PV + +S LR+L+ +F+R P ERP++N+IL K FI+K+I+KFL P+ + K Q KRP+ ++ V K A+ G D+A P + + KR+ + ++ + + V Q K ++E IN RE GW+NVLS S K +G A + + + Y+ Y+A D+I++Q+ + EG+ + + ++ +SP G + G+ + DA K +E S+ R G + A Q ++EF +RKR+A+ K R M L C + S R+ EEE RL +IR +NF RQ
Sbjct: 1 MDKYIKVQKIGEGSFGKAILVKAKENGKQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEETGCLYIVMDYCEGGDLFKKINAQKGVFFSEDQIMDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDMWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSTRYSHELRSLISQLFKRNPRERPSVNSILEKMFIVKRIEKFLTPQLIAEEFSHKMFQKFGPHAGPAKRPAQGQNLASVAPAHKITKPAAKYGVPLILKKSGDSAKKFFEKPITKCKQEAVKKKRLEEKQQREQISYMKVEQMRRLEKERMERINRAREQGWRNVLSISGSS--------EIKAQYFGGGGAIMPLPVPDRGPYEHYHAIFDQIQQQKENLKIEGKDVKLNEKPCVEPIHRRSPHGIHAGIHKGSAAYHHSSDADAIRKGMKRLEEVSRQANANRQKGWLAAERAKQ--VEEFWQRKREARQNKARAEGHMDTLQNLTAICGGRSISSRGRKARNKEEEEYLTRLRQIRLQNFNERQ 580
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: A0A2D4I310 (Protein kinase domain-containing protein (Fragment) OS=Micrurus lemniscatus lemniscatus OX=129467 PE=4 SV=1) HSP 1 Score: 377.867 bits (969), Expect = 9.194e-114 Identity = 222/574 (38.68%), Postives = 331/574 (57.67%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNETKKQIQKRPSTASVVSRVDSG---LSNKKKARVGRDNALINSPYMQIPNRNIHDKRVSPRIVKQDANPL-VVRQQELNNKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPAAIKQKI-NSPAYDRYYAELDRIKEQQVLRENEGRALM---QHMLKQNDNKSPDGKLLKV--GADGLYRKIDAS------KPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQ--SPIPRQTPRSIEEETTERLNKIRQKNFEIRQ 1783
+ + ++ KIG+G+FG A LV++K + K+YV+KEI + KM KER E+R+EV++L+ M HPNIV Y SFEE GC +I+M+YCEGGDL++KI Q GV F EDQ++D+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +++LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSD+W+LGC+LYE+C+L +AF ++ L ++I ++ PV + +S LR+L+ +F+R P ERP++N+IL K FI+K+I+KFL P+ + K QK A R G S ++ + A P + + + K I K + R +E + ++E IN RE GW+NVLS S K +G A + + + Y+ Y+A D+I++Q+ + EG+ + + ++ +SP G + G+ + DA K +E S+ R G + A Q ++EF +RKR+A+ K R M L C + S R+ EEE RL +IR +NF RQ
Sbjct: 1 MDKYIKVQKIGEGSFGKAILVKAKENGKQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEETGCLYIVMDYCEGGDLFKKINAQKGVFFSEDQIMDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDMWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSTRYSHELRSLISQLFKRNPRERPSVNSILEKMFIVKRIEKFLTPQLIAEEFSHKMFQKFGPHAGPAKRPAQGQNLASVAPAHKITKPAAKYGVPLILKKSGDSAKKFFEKPITKCKQEAVKKKRLEEKQQREQMERINRAREQGWRNVLSISGSS--------EIKAQYFGGGGAIMPLPVPDRGPYEHYHAIFDQIQQQKENLKIEGKDVKLNEKPCVEPIHRRSPHGIHAGIHKGSAAYHHSSDADAIRKGMKRLEEVSRQANANRQKGWLAAERAKQ--VEEFWQRKREARQNKARAEGHMDTLQNLTAICGGRSISSRGRKARNKEEEEYLTRLRQIRLQNFNERQ 564
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: A0A5G3JN56 (Protein kinase domain-containing protein OS=Xenopus tropicalis OX=8364 GN=nek1 PE=4 SV=1) HSP 1 Score: 382.489 bits (981), Expect = 6.877e-109 Identity = 238/650 (36.62%), Postives = 367/650 (56.46%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPEN--------PLLNETKKQIQ----KRPSTASVV-------------SRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHDKRVSPRIVKQDA------NPLVVRQQELNNKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPA-AIKQKINSPAYDRYYAELDRIKEQQVLRENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKI----------DASKPYDE----RSKMNELARIHGKIGGN------AADQA-VIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQ--SPIPRQTPRSIEEETTERLNKIRQKNFEIRQGMGFE-DFVKNSDIDGNGNFENFNQLEARKALEDLKKRV 1897
++ + R++KIG+G+FG A LV+SK D K+YV+KEIG+ KM KER E+R+EV++L+ M HPNIV Y SFEE GC +I+M+YCEGGDL+++I +Q GVLF EDQ+LD+F+Q+CLALKH+HD++ILHRDIK QNIFLTK +++LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYE+C+L +AF ++ L ++I + +Y PV +S LR L+ +F+R P +RP++N+IL KPFI ++I+KFL P++ +N K IQ KRP+ A VV ++ L+ KK + + + P ++ KR+ R ++++ N L Q K ++E IN RE GW+NV+SS S + K +G A Q + Y+ Y++ D++++Q +EN A+ + + + D ++PD Y ++ A P+ + R ++ L +H + GN +A++A ++EF++RK +A K R M + C + S + E+E RL +IR +NF RQ + + K + D +G + + RK +E LK +
Sbjct: 1 MNKYVRVSKIGEGSFGKAILVKSKDDGKQYVIKEIGISKMSNKEREESRREVAVLANMKHPNIVQYQESFEESGCLYIVMDYCEGGDLFKRINSQKGVLFSEDQILDWFVQLCLALKHVHDRKILHRDIKSQNIFLTKGGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENRPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSYDLRNLLSMLFKRNPRDRPSVNSILEKPFIYRRIEKFLSPQHIAEEFGLGAFVNHPKPGIQAAAAKRPTPAQVVPASPPAHKITKPAAKYGVPLTTKKSSDAAKKHH-DKKPQIRHRQDGAKKKRMDVRQLEKEKRQREQFNLLRAEQMRRLEKERLERINRAREQGWRNVISSGGSG--------DIKAPYFGGGGGVAPPQGMGRGPYEHYHSIFDQMQQQ---KENIKAAVEKEVKLKADIQNPD----------TYSRVPKHPEQENCPSAHSPFPDGDAVRRELKRLQEVHNQAIGNKIQGHVSAEKARQVEEFLQRKHEAMQNKARAEGHMDAVHNLAAICGGRPNSAGGGKARNREEKEYLARLRQIRLQNFNERQQIKAKLRGAKGDEGDADGKESSEDAELRRKKIEALKAQA 628
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: I3J4T0 (NIMA-related kinase 1 OS=Oreochromis niloticus OX=8128 GN=nek1 PE=4 SV=1) HSP 1 Score: 382.874 bits (982), Expect = 1.448e-108 Identity = 242/650 (37.23%), Postives = 367/650 (56.46%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE--------NPLLNETKKQIQKRPSTAS--------------VVSRVDSGL---SNKKKARVGRDNALINSPYMQIPN----RNIHDKRVSPRIV-------KQDANPLVVRQQELN--NKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAK------LNVYGMTPAAIKQKINSPAYDRYYAELDRIKE---QQVLRENE--GRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDASKPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNR------NGSGMALLMGGHQF---CAEQSPIPRQTPRSIEEETTERLNKIRQKNFEIRQGMGFEDFVKNSDIDGNGNFENFNQLEA-RKALEDLKKRV 1897
++++ KIG+G+FG A LV+SK D +YV+KEIG+ M KER E+RKEV++L+ M+HPNIV Y SFEE GC +I+M+YCEGGDL++KI +Q GVLF E+Q+LD+ +QICLALKH+HD++ILHRDIK QNIFLTK+ +V+LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYE+C+L +AF ++ L ++I + +Y PV +S LR+L+ +F+R P ERP++N+IL KPF+ +I+KFL P+ L + K + + PS ++ V +V G ++KK A + L +P ++ R H+ + + + KQ +++ +++ K K+ IN RE GWK+VLSS S ++ + V G TPA + K SP Y+ Y+A LD++ + + V R++ G + P+G ++ D + R++ SK ++R G++ A Q ++EF++R+R+A K R +A + GG C R P EEE RL +IR +NF RQ + + D DG+ + E+ + E RK +E LK +
Sbjct: 4 YEKVKKIGEGSFGKAILVKSKDDGHQYVIKEIGISGMSSKERQESRKEVAVLANMSHPNIVQYKESFEEGGCLYIVMDYCEGGDLFKKINSQKGVLFSEEQILDWLVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSCHYSQELRSLLAQLFKRNPRERPSVNSILDKPFLSCRIEKFLTPQIIAQEFRHTFLHKQPKLGVAQMPSASAQKITKPAAKYGVPLTVRKVPDGARKPADKKPAGKHKPAPLPATPQRRVSQVEEERKKHEDSIRKKRMEMIEKERKQREQMFMLKAEQMKRYEKEKINRINQAREQGWKHVLSSSGGSSPERKCFVGGGKRGAHVAAVQGSTPAPLPGK--SP-YEHYHAILDQMAKPTPKDVSRKSSSAGPCSPIRVPAAAGPVLPNGPARRLDPDVIKRELHR---LQHVSKQAHISRQRGQMAAERAFQ--VEEFLQRRREAMLNKARAEGQLGTRQNLAAIYGGRAGGVRCG------RSKPNKEEEEYLARLRQIRLQNFNERQQIKARLRGEKYDSDGSDSQESSEEAELRRKKIEALKAQA 639
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: A0A2Y9Q561 (serine/threonine-protein kinase Nek1 isoform X6 OS=Delphinapterus leucas OX=9749 GN=NEK1 PE=4 SV=1) HSP 1 Score: 376.711 bits (966), Expect = 5.887e-107 Identity = 242/652 (37.12%), Postives = 364/652 (55.83%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNE-----------TKKQIQKRPSTA-SVVSRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHD---------------KRVSPRIVKQD--ANPLVVRQQELNNKYK---------------------VEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPAAIKQKINSPA-YDRYYAELDRIKEQQVLRENEGRALMQ-HMLKQNDNKSPDG--KLLKVGADGLYRKIDAS---KPYDERSKMNELARIHGKIGGNAADQA-VIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQSPIPR-QTPRSIEEET-TERLNKIRQKNFEIRQGMGFEDFVKNSDIDGNGNFENFNQLEA-RKALEDLKKRV 1897
+ R+ KIG+G+FG A LV+S D ++YV+KEI + M KER E+R+EV++L+ M HPNIV Y SFEE G +I+M+YCEGGDL+++I Q G+LF+EDQ+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +++LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYE+C+L +AF ++ L ++I ++ PV +S LR+L+ +F+R P +RP++N+IL K FI K+I+KFL P+ L+ E T+ KRP + S+ S V + K A+ G + Y + ++ +H+ K+V+P ++ P R+ ELN K K +E IN RE GW+NVLS+ S K ++G A +S Y+ Y+A D++ +QQ +NE + + H + + P G GA G + DA K + +++ A I+ + G A ++A ++EF++R+R+A+ K R M L C + R PRS EEE RL +IR +NF RQ + + + + D + E + + RK +E LK +V
Sbjct: 4 YVRVQKIGEGSFGKAILVKSIEDGRQYVIKEINISSMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGILFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELVRTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLISQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQ--LIAEEFCLKTFSRFGTQPIPAKRPGSGQSLASVVSAQKITKPAAKYG-----VPLTYKKYGDKKLHEKKPLQKHKQAHQSPVKKVNPGEERRKLFEEPARKRRLELNEKEKKQKDQISLMKAEQMKRQEKERLEKINRAREQGWRNVLSAGGSG--------EMKAPLFGSGGAVAPSSFSSRGQYEHYHAIFDQM-QQQRAEDNEAKWKGERHGRRLPERGIPPGVHPGFPKGAAGHHHSSDADALRKTWKRLKAVSKQANINRQKGQLAVERAKQVEEFLQRRREARQNKARAEGHMGTLHNLAALCEGRRSSSRGGKPRSKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKKEADSSEGQEGSEEADVRRKKVEVLKAQV 639
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: nek1 (NIMA related kinase 1 [Source:NCBI gene;Acc:103028272]) HSP 1 Score: 356.681 bits (914), Expect = 8.448e-103 Identity = 180/398 (45.23%), Postives = 261/398 (65.58%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE-------NPLLNETKKQIQ----KRPSTASV-----------VSRVDSGLSNKKKARVGR---DNA---LINSPYMQIPNRNIHDKRVS--PRIVKQDANPLVVRQQELN--NKYKVEMINVDRENGWKNVL 1222
++++ KIG+G+FG A LV SK D +YV+KEIG+ +M KER E+RKEV++L+ M+HPNIV Y SFEE GC +I+M+YCEGGDL++KI Q GVLF E+Q+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +++LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYE+C+L +AF ++ L ++I + +Y PV +S LR+L+ +F+R P ERP++ TIL KPF+ +I +FL P+ LL + K Q KRP+ ++ L+ K+ A GR D+A + P +++ + KR+ R KQ +++ ++ + K+ IN RE GW++VL
Sbjct: 4 YEKVKKIGEGSFGKAILVRSKEDGHQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEEGGCLYIVMDYCEGGDLFKKINNQKGVLFTEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSMHYSQDLRSLLGLLFKRNPRERPSVCTILDKPFLAHRIHRFLSPQLIAQEFSQSLLRQPKTPAQGLPAKRPAPGPSPITPAQKITKPAAKYGVPLTVKRPADAGRKVADSAKKQVERKPGIKLKQEGVRKKRMELMERERKQREQMFILKAAQVKRFEREKLNRINRAREQGWRHVL 401
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: nek1 (NIMA related kinase 1 [Source:NCBI gene;Acc:103028272]) HSP 1 Score: 348.591 bits (893), Expect = 6.138e-100 Identity = 151/266 (56.77%), Postives = 210/266 (78.95%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE 922
++++ KIG+G+FG A LV SK D +YV+KEIG+ +M KER E+RKEV++L+ M+HPNIV Y SFEE GC +I+M+YCEGGDL++KI Q GVLF E+Q+LD+F+QICLALKH+HD++ILHRDIK QNIFLTK+ +++LGDFGIA+ ++ST L +T +GTP+Y SPEICE PYNNKSDIW+LGC+LYE+C+L +AF ++ L ++I + +Y PV +S LR+L+ +F+R P ERP++ TIL KPF+ +I +FL P+
Sbjct: 4 YEKVKKIGEGSFGKAILVRSKEDGHQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEEGGCLYIVMDYCEGGDLFKKINNQKGVLFTEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSMHYSQDLRSLLGLLFKRNPRERPSVCTILDKPFLAHRIHRFLSPQ 269
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000049860.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000129.1:61559:73217:-1 gene:ENSAMXG00000043136.1 transcript:ENSAMXT00000049860.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 306.22 bits (783), Expect = 4.271e-89 Identity = 153/329 (46.50%), Postives = 215/329 (65.35%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLV-ESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNS-VKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE-------NPLLNETKKQIQKRP--STASVVSRVDSGLSNKKKARVGRDNALINSPYMQ 1069
+++++ + +IG GA+G A LV E + + V+K+I +M +ER +++KEV+LLSKM HPNIV ++ SF E +I+MEYC+GGDL +KI Q G FKE Q++D+F+QICL LKHIHD+++LHRDIK QNIFLT+ S VKLGDFGIA+ +++T L +T VGTP+Y SPEICE PYNNK+D+WSLGC+LYELC+L + F G + L +RI K Y PV +S+ LR LV +F+ P +RP++N++L++P + I K L P+ + +L+ K Q P S S VS G NK R A+ P Q
Sbjct: 1 MNEYELVRQIGHGAYGKALLVRERSGNARHCVIKQISWSRMSPREREDSKKEVTLLSKMKHPNIVAFFRSFNERNSLYIVMEYCDGGDLMKKISMQRGQPFKEQQIMDWFVQICLGLKHIHDRKVLHRDIKAQNIFLTQGGSKVKLGDFGIARLLNNTVELARTCVGTPYYLSPEICENRPYNNKTDMWSLGCVLYELCTLRHPFEGSSLRQLIVRICKGYYTPVSQRYSSDLRTLVSQLFKISPRDRPSVNSLLKRPLLHTLISKHLHPQLMEEEFSHTVLHRVKPVAQPGPKHSAVSKVSPRSGGAVNKLPPRPPWRRAVTKPPSPQ 329
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000040192.1 (NIMA related kinase 3 [Source:NCBI gene;Acc:103034307]) HSP 1 Score: 260.766 bits (665), Expect = 2.275e-76 Identity = 123/261 (47.13%), Postives = 177/261 (67.82%), Query Frame = 2
Query: 143 IGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPEN 925
IG G+FG A LV+++ +K+V+KEI L K R R+E LL+KM H NIV + SFE+ G I+ME+C GGDL ++I+ Q G LF ED +L +F Q+CL KHIHD+R+LHRD+K +NIFLT++ ++KLGDFG A ++S A +T VGTP+Y SPEI + PYNNKSD+WSLGC+LYELC+L++ F + L +++ + +Y P+P L L+ +MF+ P +RP+I+TIL + + + K LP E+
Sbjct: 10 IGTGSFGRALLVQTEGRGEKWVMKEIRLPKAESGMR-TTRREAVLLAKMKHSNIVAFRESFEDDGHLFIVMEFCSGGDLLQRIRQQKGSLFSEDVILRWFSQMCLGTKHIHDKRVLHRDLKSKNIFLTEHGTIKLGDFGSACTLNSARAYAQTYVGTPYYVSPEIWDNQPYNNKSDVWSLGCVLYELCTLHHPFQSRSWKNLILKVCRGSYAPLPRHLPYELHYLIKHMFKTNPRDRPSIHTILNSHRVSRLLLKHLPEES 269
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000010177.2 (NIMA related kinase 3 [Source:NCBI gene;Acc:103034307]) HSP 1 Score: 260.381 bits (664), Expect = 5.347e-76 Identity = 123/261 (47.13%), Postives = 177/261 (67.82%), Query Frame = 2
Query: 143 IGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPEN 925
IG G+FG A LV+++ +K+V+KEI L K R R+E LL+KM H NIV + SFE+ G I+ME+C GGDL ++I+ Q G LF ED +L +F Q+CL KHIHD+R+LHRD+K +NIFLT++ ++KLGDFG A ++S A +T VGTP+Y SPEI + PYNNKSD+WSLGC+LYELC+L++ F + L +++ + +Y P+P L L+ +MF+ P +RP+I+TIL + + + K LP E+
Sbjct: 10 IGTGSFGRALLVQTEGRGEKWVMKEIRLPKAESGMR-TTRREAVLLAKMKHSNIVAFRESFEDDGHLFIVMEFCSGGDLLQRIRQQKGSLFSEDVILRWFSQMCLGTKHIHDKRVLHRDLKSKNIFLTEHGTIKLGDFGSACTLNSARAYAQTYVGTPYYVSPEIWDNQPYNNKSDVWSLGCVLYELCTLHHPFQSRSWKNLILKVCRGSYAPLPRHLPYELHYLIKHMFKTNPRDRPSIHTILNSHRVSRLLLKHLPEES 269
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007414.1 (pep scaffold:Pmarinus_7.0:GL476981:230882:244405:1 gene:ENSPMAG00000006689.1 transcript:ENSPMAT00000007414.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 313.538 bits (802), Expect = 9.223e-98 Identity = 137/266 (51.50%), Postives = 198/266 (74.44%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE 922
+ +++IG+G+FG A LV+SK D YV+KEI + +M KER EARKEV +L+ M HPNIV+Y SFEE G +I+M+YCEGGDL+++I Q GVLF EDQ+LD+ +Q+CLALKH+HD++ILHRDIK QNIFLTK V+LGDFGIA+ ++STT L +T +GTP+Y SPEICE PYN+KSD+W+LGC+LYE+C+L +AF ++ L ++I + ++ PV + +S+ LR+ + + RR P RP + +L +P ++ + + + E
Sbjct: 4 YTMVSQIGEGSFGKAMLVKSKADGHLYVIKEINISRMSPKERDEARKEVCVLANMRHPNIVSYKESFEERGNLYIVMDYCEGGDLFKRINAQKGVLFSEDQILDWLVQLCLALKHVHDRKILHRDIKSQNIFLTKAGIVQLGDFGIARVLNSTTELARTCIGTPYYLSPEICENKPYNHKSDMWALGCVLYEMCTLRHAFEAGNMKNLVLKIIRGSFPPVSAHYSSELRSTLSGLLRRDPRARPPVAVLLSRPLLMARAQRLMVSE 269
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Match: NEK9 (NIMA related kinase 9 [Source:HGNC Symbol;Acc:HGNC:18591]) HSP 1 Score: 195.667 bits (496), Expect = 6.822e-52 Identity = 101/248 (40.73%), Postives = 152/248 (61.29%), Query Frame = 2
Query: 143 IGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRI----KKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTIL 874
+G+GAFG A L D V KEI L ++ +KER +++ E+ +LS + H NI++YYN F + I +EYC GG LY KI Q+G F E ++ YF Q+ A++ IH+ +ILHRDIK NIFLTK++ +K+GDFGI+K +DS + + ++ VGTP+Y SPE+ G YN KSDIW++GC+LYEL +L F + L I K+V + +S ++ +V ++ ++RP +L
Sbjct: 42 LGRGAFGEAALYRRNEDNSLVVWKEIDLTRLSDKERRDSQNEIDILSLLEHANIISYYNHFLDGNVLLIELEYCNGGTLYEKIIKQHGAFFPEKDIVWYFYQLVSAVEQIHNFKILHRDIKTLNIFLTKSDLIKVGDFGISKILDSRSQMAESYVGTPYYMSPELIRGDKYNTKSDIWAMGCVLYELLTLTRTFDATNPLKLCYEIIHGKKQVE---IDDQYSKEIKEVVQKCLQQDAEQRPTAEDLL 286
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000555.1 (pep scaffold:Pmarinus_7.0:GL477093:35206:42652:1 gene:ENSPMAG00000000500.1 transcript:ENSPMAT00000000555.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 166.392 bits (420), Expect = 1.495e-47 Identity = 74/173 (42.77%), Postives = 115/173 (66.47%), Query Frame = 2
Query: 410 LFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMR-----IKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFL 913
+ + V+ +F+Q+C+A+++IH +ILHRDIK N+FLTK +VK+GDFGI+KAM+ T + T VGTP+Y SPE+C+ PY++KSD+W+LGC+LYELC+L AF +I L R I + P+ +S +RALV + R P++RP+ + +L P+ + + F+
Sbjct: 4 IITKTNVMQWFVQVCMAVQYIHSVKILHRDIKTSNVFLTKAGTVKVGDFGISKAMEGTLDMAVTCVGTPYYLSPEMCQDLPYSSKSDVWALGCLLYELCALRPAFEASNIIRLTRRSLFYKIMRGEPDPLDESYSRDVRALVALLLSRSPEDRPSASAVLASPYAQRHLRLFV 176
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001750.1 (pep scaffold:Pmarinus_7.0:GL481695:1137:12922:1 gene:ENSPMAG00000001576.1 transcript:ENSPMAT00000001750.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 172.555 bits (436), Expect = 2.481e-45 Identity = 83/182 (45.60%), Postives = 113/182 (62.09%), Query Frame = 2
Query: 368 GGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSV-KLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKF 910
GG L I+ + G L ED +L +F+QI LAL H+H ILHRD+K NI L K +V K+GDFGI+K + S + T VGTP Y SPE+CEG PYN KSDIW+LGCILYEL SL AF +++ L ++I N+ PV +S LR LV ++ P +RP ++ +L P + + +
Sbjct: 1 GGTLAEYIQKRGGSLLDEDTILHFFVQIALALHHVHSNHILHRDLKTHNILLDKRQTVVKIGDFGISKILSSKSK-AYTVVGTPCYISPELCEGKPYNQKSDIWALGCILYELASLKRAFEAENLPALVLKIMSGNFAPVSDRYSGELRQLVVSLLNLDPSKRPQLSEVLSHPVCMSAMIRL 181
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Match: nek2 (NIMA-related kinase 2 [Source:ZFIN;Acc:ZDB-GENE-040426-752]) HSP 1 Score: 165.622 bits (418), Expect = 2.832e-44 Identity = 93/244 (38.11%), Postives = 138/244 (56.56%), Query Frame = 2
Query: 233 MHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVG--CFHILMEYCEGGDLYRKIKT--QNGVLFKEDQVLDYFIQICLALKHIH-----DQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDK---FLPPENP 928
M E E+ EV+LL ++ HPNIV Y++ + +I+MEYCE GDL I ++ +E+ VL Q+ LAL+ H + +LHRD+KP N+FL ++VKLGDFG+A+ + T+ KT VGTP+Y SPE YN KSDIWSLGC+LYELC+L F + LA +IK+ ++ +P +S L ++ M + RP+I I++ + + +D+ PE P
Sbjct: 13 MSEPEKQMLVSEVNLLRELKHPNIVRYFDRIIDRSNTTLYIVMEYCECGDLASLISRCRRDRRFLEEEFVLRVLAQLVLALRECHRRTAGGRTVLHRDLKPANVFLDARHNVKLGDFGLARILQHDTSFAKTFVGTPYYMSPEQFSRMSYNEKSDIWSLGCLLYELCALVPPFTAMNQKQLAEKIKEGKFRRIPYRYSDELNEIISKMLNLKDYLRPSIEQIMQYGLLAEVLDEEQARAAPEKP 256
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: KIN3 (Nonessential serine/threonine protein kinase; possible role in DNA damage response; influences tolerance to high levels of ethanol [Source:SGD;Acc:S000000071]) HSP 1 Score: 144.821 bits (364), Expect = 2.508e-37 Identity = 117/389 (30.08%), Postives = 174/389 (44.73%), Query Frame = 2
Query: 119 SDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYN-SFEEVG-CFHILMEYCEGGDLYRKIK--TQNGVLFKEDQVLDYFIQICLALKHIH---------------------DQRILHRDIKPQNIFLTKNNS---------------------------------------VKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTIL--------RKPFILKKID-KFLPPENPLLN----------ETKKQIQ--KRPSTASVVSRVDSGLSNKKKARV 1030
S+++ L +IG+G+FG V K V K+I M+ KER + E S+LS++ H NIV +YN F+E ++ MEYC GDL + IK Q E V Q+ AL H ++HRD+KP NIFL+ ++S VKLGDFG+AK+++++ T VGTP+Y SPE+ PY+ SDIWSLGC+++E+CSL+ F ++ L +IK VP +S L A++ +M RP+ +L RK L++ + K L EN L N E ++++ K T +V R +S KK +V
Sbjct: 23 SEYQVLEEIGRGSFGSVRKVIHIPTKKLLVRKDIKYGHMNSKERQQLIAECSILSQLKHENIVEFYNWDFDEQKEVLYLYMEYCSRGDLSQMIKHYKQEHKYIPEKIVWGILAQLLTALYKCHYGVELPTLTTIYDRMKPPVKGKNIVIHRDLKPGNIFLSYDDSDYNINEQVDGHEEVNSNYYRDHRVNSGKRGSPMDYSQVVVKLGDFGLAKSLETSIQFATTYVGTPYYMSPEVLMDQPYSPLSDIWSLGCVIFEMCSLHPPFQAKNYLELQTKIKNGKCDTVPEYYSRGLNAIIHSMIDVNLRTRPSTFELLQDIQIRTARKSLQLERFERKLLDYENELTNIEKILEKQAIEYERELSQLKEQFTQAVEERAREVISGKKVGKV 411
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: TPK3 (cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication [Source:SGD;Acc:S000001649]) HSP 1 Score: 134.035 bits (336), Expect = 5.085e-34 Identity = 78/248 (31.45%), Postives = 130/248 (52.42%), Query Frame = 2
Query: 113 SISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFE-ARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDER 853
S+SDF+ L +G G+FG L+ S + + Y LK + + + + ++ E E +LS ++HP I+ + +F++ ++M+Y EGG+L+ ++ F Y ++CLAL+++H + I++RD+KP+NI L KN +K+ DFG AK + +T T GTP Y +PE+ PYN D WS G ++YE+ + FY + I K P F + L+ + R ER
Sbjct: 84 SLSDFQILRTLGTGSFGRVHLIRSNHNGRFYALKTLKKHTIVKLKQVEHTNDERRMLSIVSHPFIIRMWGTFQDSQQVFMVMDYIEGGELFSLLRKSQ--RFPNPVAKFYAAEVCLALEYLHSKDIIYRDLKPENILLDKNGHIKITDFGFAKYVPD---VTYTLCGTPDYIAPEVVSTKPYNKSVDWWSFGVLIYEMLAGYTPFYNSNTMKTYENILNAELK-FPPFFHPDAQDLLKKLITRDLSER 325
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: TPK1 (cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; phosphorylates and inhibits Whi3p to promote G1/S phase passage; partially redundant with Tpk2p and Tpk3p; phosphorylates pre-Tom40p, which impairs its import into mitochondria under non-respiratory conditions; TPK1 has a paralog, TPK3, that arose from the whole genome duplication [Source:SGD;Acc:S000003700]) HSP 1 Score: 130.183 bits (326), Expect = 9.365e-33 Identity = 76/250 (30.40%), Postives = 132/250 (52.80%), Query Frame = 2
Query: 113 SISDFKRLNKIGQGAFGIAWLVESKIDCKKY---VLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDER 853
S+ DF+ L +G G+FG L+ S+ + + Y VLK+ + ++ + E E +LS + HP I+ + +F++ ++M+Y EGG+L+ ++ F Y ++CLAL+++H + I++RD+KP+NI L KN +K+ DFG AK + +T T GTP Y +PE+ PYN D WS G ++YE+ + FY + +I + P F+ ++ L+ + R +R
Sbjct: 83 SLQDFQILRTLGTGSFGRVHLIRSRHNGRYYAMKVLKKEIVVRLKQVEH--TNDERLMLSIVTHPFIIRMWGTFQDAQQIFMIMDYIEGGELFSLLRKSQ--RFPNPVAKFYAAEVCLALEYLHSKDIIYRDLKPENILLDKNGHIKITDFGFAKYVPD---VTYTLCGTPDYIAPEVVSTKPYNKSIDWWSFGILIYEMLAGYTPFYDSNTMKTYEKILNAELR-FPPFFNEDVKDLLSRLITRDLSQR 324
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: CDC15 (Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress [Source:SGD;Acc:S000000072]) HSP 1 Score: 131.724 bits (330), Expect = 1.719e-31 Identity = 78/249 (31.33%), Postives = 138/249 (55.42%), Query Frame = 2
Query: 143 IGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFI 889
IG+G++G+ + +K + +KE+ +Y+ +++E + E+SLL +NH NIV Y+ + +IL+EYC G L R++ +++ E++ Y Q L LK++H + ++HRDIK NI L+ +N+VKL DFG++ ++S+ T GT + +PEI + SDIWSLG + E+ + N ++ + ++ Y P PS FS PL+ + F + +RP + +L+ +I
Sbjct: 31 IGRGSYGVVYKAINKHTDQVVAIKEV-VYE-NDEELNDIMAEISLLKNLNHNNIVKYHGFIRKSYELYILLEYCANGSL-RRLISRSSTGLSENESKTYVTQTLLGLKYLHGEGVIHRDIKAANILLSADNTVKLADFGVSTIVNSSAL---TLAGTLNWMAPEILGNRGASTLSDIWSLGATVVEMLTKNPPYHNLTDANIYYAVENDTYYP-PSSFSEPLKDFLSKCFVKNMYKRPTADQLLKHVWI 272
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: CDC5 (Polo-like kinase; controls targeting and activation of Rho1p at cell division site via Rho1p guanine nucleotide exchange factors; regulates Spc72p; also functions in adaptation to DNA damage during meiosis; regulates the shape of the nucleus and expansion of the nuclear envelope during mitosis; similar to Xenopus Plx1 and S. pombe Plo1p; human homologs PLK1, PLK3 can each complement yeast cdc5 thermosensitive mutants [Source:SGD;Acc:S000004603]) HSP 1 Score: 129.798 bits (325), Expect = 2.998e-31 Identity = 78/255 (30.59%), Postives = 135/255 (52.94%), Query Frame = 2
Query: 122 DFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKM-HEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEG--CPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYK-PVPSVFSAPLRALVDNMFRRRPDERPNINTIL 874
D+ R + +G+G F + ++ + + K + + EK R + E+ + M+HPNIV + + FE+ +IL+E C G L +K + + E +V + QIC A+K++H +R++HRD+K NIF N ++K+GDFG+A + + + T GTP Y +PE+ G ++ + DIWSLG +LY L F +D+ + RIK ++ P S + L+ ++ P ERP++ I+
Sbjct: 81 DYHRGHFLGEGGFARCFQIKDD-SGEIFAAKTVAKASIKSEKTRKKLLSEIQIHKSMSHPNIVQFIDCFEDDSNVYILLEICPNGSLMELLKRRK--VLTEPEVRFFTTQICGAIKYMHSRRVIHRDLKLGNIFFDSNYNLKIGDFGLAAVLANESERKYTICGTPNYIAPEVLMGKHSGHSFEVDIWSLGVMLYALLIGKPPFQARDVNTIYERIKCRDFSFPRDKPISDEGKILIRDILSLDPIERPSLTEIM 332
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO49822 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG20]) HSP 1 Score: 323.939 bits (829), Expect = 5.450e-102 Identity = 145/266 (54.51%), Postives = 203/266 (76.32%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPE 922
+ R+ KIG+G+FG A LV+ + D K YV+KEI + +M +ER E+RKEV +LS++ HPNIV+Y SFEE G +I+M+YC+GGDLY+KI Q G+ F EDQV+D+F+QICL LKH+HD++ILHRDIK QNIFLT++ +K+GDFGIA+ + ST L +T +GTP+Y SPEI E PYNNKSDIWSLGC+LYE+ +L +AF ++ L ++I + +Y P+P +SA +R LV + +R P +RP++NT+L+K FI K+I+KFL E
Sbjct: 4 YVRVKKIGEGSFGKALLVKKRNDGKHYVIKEINICRMKPREREESRKEVKVLSQLKHPNIVSYQESFEESGNLYIVMDYCDGGDLYKKINGQRGIAFSEDQVMDWFVQICLGLKHVHDRKILHRDIKSQNIFLTRHGIIKMGDFGIARVLHSTVELARTCIGTPYYLSPEIVENRPYNNKSDIWSLGCVLYEMLTLKHAFEAGNMKNLVLKIIRGSYPPIPLRYSADIRMLVAQLLKRNPHDRPSVNTVLKKNFIQKRIEKFLSKE 269
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO49138 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RH03]) HSP 1 Score: 260.766 bits (665), Expect = 3.591e-79 Identity = 126/262 (48.09%), Postives = 176/262 (67.18%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKF 910
+++++ IG+G FG AWLVES+ + Y LKE+ M ++R A EV +LS + H NIV Y ++FEE G F+I+MEY EGGDL+ KIK Q F +++L++ Q+ LALKHIH Q ILHRD+K QNIFLTK++ VK+GDFGIA+ +DST +T VGTP+Y SPEIC+ PYNNKSDIW+LGC+LYEL + + F ++T L ++I NY P+P + L LV M + P +RP+ ++ P + IDK+
Sbjct: 4 YQKIDIIGKGTFGSAWLVESRQSKRLYALKELNATAMPSEDRHLALNEVKILSTLKHRNIVRYRDAFEETGRFYIVMEYAEGGDLHTKIK-QAVEPFSSERILNWLAQLLLALKHIHGQNILHRDLKTQNIFLTKDDVVKIGDFGIARILDSTCDHARTTVGTPYYLSPEICQRQPYNNKSDIWALGCVLYELTTRTHPFTADNLTNLVVKILHGNYPPIPRFYGPLLEDLVAVMLKINPADRPSAKQLIYVPGLTPYIDKY 264
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO49690 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG02]) HSP 1 Score: 258.07 bits (658), Expect = 5.821e-78 Identity = 116/258 (44.96%), Postives = 183/258 (70.93%), Query Frame = 2
Query: 143 IGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEE-VGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFL 913
IG+G++G +L + K D K+YVLK++ L K +ER A E LLS++ HPNIV+Y SF++ G +I M +CEGGDLY ++K Q G+ E+Q++++F+QI +AL+++H++ ILHRD+K QNIFLTK+ +K+GD GIA+ ++S++ + T +GTP+Y SPE+ PYN+KSD+W+LGC LYE+C+L +AF +D++ L +I K P+P +S L +++ +M + PD+RP+ + +LR P+I K+I FL
Sbjct: 12 IGKGSYGEVFLSKHKKDKKQYVLKKVDLQKASVRERKAAELEAKLLSQLRHPNIVSYRESFQDDTGFLYIAMNFCEGGDLYSRLKAQKGIPLDENQIVEWFVQIAMALQYMHEKHILHRDLKTQNIFLTKSKIIKVGDLGIARVLESSSDMATTLIGTPYYMSPELFSNKPYNHKSDVWALGCCLYEMCTLRHAFNAKDMSSLVYKILKGKTPPLPKQYSTDLCSIIKSMLDQDPDKRPSASRLLRHPYIKKQIALFL 269
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO37069 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SGU2]) HSP 1 Score: 225.328 bits (573), Expect = 1.501e-66 Identity = 116/263 (44.11%), Postives = 172/263 (65.40%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVES-KIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKT--QNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKID 904
++ + ++G G+FG +LVE K + ++ VLKEI + ++ E +A +E LLSK+NHPNIV + +SF + F I+ EYCEGGDL KI ++G F E +L +FIQI LA++H+H+ RILHRD+K +NIFL KNN VKLGDFGI++ + T+ + T GTP+Y SPE+ + YN+KSDIWSLG +LYELC+L +AF GQ + + +I + + +S+ L+A + + P RP+ N IL+ FI + I+
Sbjct: 6 YRVVRRLGSGSFGTVFLVEDLKANGERKVLKEIYVGELQPDETVDAMREAKLLSKLNHPNIVKFMDSFMDREYFCIVTEYCEGGDLDDKISAWRKSGKKFDESLILAWFIQIVLAVQHMHEMRILHRDLKTRNIFL-KNNIVKLGDFGISRILMGTSDMATTFTGTPYYMSPEVLKHEGYNHKSDIWSLGVVLYELCNLQHAFQGQSLMGVMYKIVEGELPALSDKYSSQLQAFYSRLMDKDPKLRPSANEILKHHFIAENIN 267
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO46185 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQY7]) HSP 1 Score: 226.868 bits (577), Expect = 9.380e-63 Identity = 108/263 (41.06%), Postives = 168/263 (63.88%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNS-VKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKI 901
+ +++++ +G+GA+G +L +D ++K+I + +M ++ER A EV +LS HPNI+ YY+SF + I+MEY +GG +Y ++ + G L ED++L F+QI LAL+H+H +ILHRD+K QNI L K VK+GDFGI+K + S + T +G+P Y SPE+CEG PYN KSD+WSLGC+LYEL +L AF ++ L ++I + + P+P +S LR L+ +M P +RP I I+ +P I+ +
Sbjct: 1 MQNYEKVRIVGRGAYGTVYLCRRLVDNFLVIIKQIPVEEMTKEERQSALNEVKVLSMFQHPNIIRYYDSFVQEKALMIVMEYAQGGTIYDYLQQRGGKLMDEDEILRLFVQILLALRHVHKGQILHRDLKTQNILLNKKRKVVKIGDFGISKILSSKSK-ANTVIGSPCYISPELCEGKPYNQKSDVWSLGCVLYELTTLKRAFEASNLPALVLKIMRGYFSPIPERYSEELRKLILDMLVLDPTKRPGIKEIMAQPVIVNAL 262
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000000545.2 (pep primary_assembly:ASM223467v1:4:1040495:1044496:1 gene:ENSORLG00000000439.2 transcript:ENSORLT00000000545.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 321.242 bits (822), Expect = 8.966e-101 Identity = 149/263 (56.65%), Postives = 206/263 (78.33%), Query Frame = 2
Query: 125 FKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFL 913
++ + +IG+G+FG A LV SK D ++YV+KEIG+ M KER E+RKEVS+L+ M+HPNIV Y SFE GC +I+M+YCEGGDL++KI +Q GVLF EDQ+LD+F+QICLALKH+H ++ILHRDIK QNIFLTK+ +V+LGDFGIA+ ++ T L +T +GTP+Y SPEICE PYNNK DIW+LGC+LYE+C+L +AF ++ L ++I + +Y PV +S LR+L+ +FRR P ERP++++IL KPF+ +I+KFL
Sbjct: 4 YENVKQIGEGSFGKAILVNSKEDGRQYVIKEIGISGMSTKERRESRKEVSVLANMSHPNIVQYKESFEG-GCLYIVMDYCEGGDLFKKINSQKGVLFSEDQILDWFVQICLALKHVHGRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLN-TVELARTCIGTPYYLSPEICENKPYNNK-DIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSPELRSLLAQLFRRNPRERPSVSSILEKPFLSCRIEKFL 263
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000034147.1 (serine/threonine-protein kinase Nek5 [Source:NCBI gene;Acc:101162253]) HSP 1 Score: 304.679 bits (779), Expect = 9.796e-89 Identity = 140/267 (52.43%), Postives = 197/267 (73.78%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDC--KKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNN-SVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDK 907
++D++ L +IG+GAFG A+LV SK + V+K+I L KM KER +RKEV+LLS+M HPNIV++ +SF E G I+ME+C+GGDL +KI Q GV F+E+++LD F+QICL LKHIHD+++LHRDIK QNIFLT KLGDFGIA+ ++++ L +T VGTP+Y SPEICE PYNNK+D+WSLGC+LYELC+L + F G + L +I + Y PVP+ ++ LR LV+ +F+ P +RP+I+++L++PF+ K I K
Sbjct: 1 MNDYEVLWQIGEGAFGKAFLVRSKTGGGDTQCVIKQINLQKMSAKERESSRKEVTLLSRMRHPNIVSFISSFLEKGSLFIVMEFCDGGDLLKKINMQRGVPFREEEILDLFVQICLGLKHIHDKKVLHRDIKCQNIFLTNGGMKAKLGDFGIARMLNNSMDLARTCVGTPYYLSPEICESRPYNNKTDVWSLGCVLYELCTLRHPFEGSSLRQLLGKICRGRYAPVPTRYTHELRLLVNQLFKVNPRDRPSISSVLQRPFLQKHISK 267
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: nek4 (NIMA related kinase 4 [Source:NCBI gene;Acc:101169778]) HSP 1 Score: 252.677 bits (644), Expect = 9.558e-70 Identity = 113/258 (43.80%), Postives = 179/258 (69.38%), Query Frame = 2
Query: 143 IGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFE-EVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFL 913
+G+G++G LV+ K D K+YV+K++ L ++ER A +E LLS++ HPNIVTY S+E + +I+M +CEGGDLY ++K + G L E QV+++F+QI +AL+++H++ ILHRD+K QNIFLTK N +K+GD GIA+ +++ + T +GTP+Y SPE+ PYN+KSD+W+LGC +YE+ +L +AF +D+ L RI + +PS + L L+ +M ++P+ERP++ ILR+P+I ++I FL
Sbjct: 10 VGKGSYGEVNLVKHKTDRKQYVIKKLNLSTSSKRERRSAEQEAQLLSQLRHPNIVTYRESWEGDDRQLYIVMGFCEGGDLYHRLKQRKGELLPERQVVEWFVQIAMALQYLHERNILHRDLKTQNIFLTKTNIIKVGDLGIARVLENQNDMASTLIGTPYYMSPELFSNKPYNHKSDVWALGCCVYEMSTLKHAFNAKDMNSLVYRIVEGKLPQMPSRYDPQLGDLIKSMLSKKPEERPDVKLILRQPYIKRQIAMFL 267
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000027525.1 (NIMA related kinase 3 [Source:NCBI gene;Acc:101171427]) HSP 1 Score: 241.506 bits (615), Expect = 1.956e-69 Identity = 117/267 (43.82%), Postives = 168/267 (62.92%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLP 916
+ + +L +G+G+FG A LV+ +KYV+KEI L K K R ++RKE LLS+M HPNIV + +FE I+MEYC GGDL ++I+ Q F + +L +F Q+C +HIHD+R+LHRD+K +NIFLT + SVKLGDFG A ++S+ A VGTP+Y +PEI + PYNNKSD+WSLGC+LYELC+L + F L +++ + Y P+P L L+ +F+ P +RP++ TIL + K + LP
Sbjct: 1 MEKYTQLKVVGEGSFGRAVLVQWISTQEKYVVKEIQLPKNESKLR-DSRKEAILLSRMKHPNIVAFREAFEMTDLLCIVMEYCSGGDLLQRIQQQKATNFCTENILRWFAQMCAGAQHIHDKRVLHRDLKSKNIFLTGDGSVKLGDFGSACVLNSSKAYAHAYVGTPYYVAPEIWDNKPYNNKSDVWSLGCVLYELCALQHPFQASSWKSLILKVCRGAYPPLPRHLPYELLHLMKQIFKTNPKDRPSVQTILTSHRVSKLLRSHLP 266
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000045610.1 (NIMA related kinase 3 [Source:NCBI gene;Acc:101171427]) HSP 1 Score: 241.506 bits (615), Expect = 1.956e-69 Identity = 117/267 (43.82%), Postives = 168/267 (62.92%), Query Frame = 2
Query: 116 ISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLP 916
+ + +L +G+G+FG A LV+ +KYV+KEI L K K R ++RKE LLS+M HPNIV + +FE I+MEYC GGDL ++I+ Q F + +L +F Q+C +HIHD+R+LHRD+K +NIFLT + SVKLGDFG A ++S+ A VGTP+Y +PEI + PYNNKSD+WSLGC+LYELC+L + F L +++ + Y P+P L L+ +F+ P +RP++ TIL + K + LP
Sbjct: 1 MEKYTQLKVVGEGSFGRAVLVQWISTQEKYVVKEIQLPKNESKLR-DSRKEAILLSRMKHPNIVAFREAFEMTDLLCIVMEYCSGGDLLQRIQQQKATNFCTENILRWFAQMCAGAQHIHDKRVLHRDLKSKNIFLTGDGSVKLGDFGSACVLNSSKAYAHAYVGTPYYVAPEIWDNKPYNNKSDVWSLGCVLYELCALQHPFQASSWKSLILKVCRGAYPPLPRHLPYELLHLMKQIFKTNPKDRPSVQTILTSHRVSKLLRSHLP 266
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000020466.1 (SMESG000020466.1) HSP 1 Score: 2123.21 bits (5500), Expect = 0.000e+0 Identity = 1124/1124 (100.00%), Postives = 1124/1124 (100.00%), Query Frame = 2
Query: 107 MSSISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNETKKQIQKRPSTASVVSRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHDKRVSPRIVKQDANPLVVRQQELNNKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPAAIKQKINSPAYDRYYAELDRIKEQQVLRENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDASKPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQSPIPRQTPRSIEEETTERLNKIRQKNFEIRQGMGFEDFVKNSDIDGNGNFENFNQLEARKALEDLKKRVXXXXXXXXXXXXXXXXXXXXXLPSVIDDDPPNNAILFPKRPPVVVNKPENSSNVSHPIAEPGIISVILERIHENITPAGDAPKVDNIELPESLSVILSQLDDKSRPSTASCSDQSEIRKRKDDILKRLNMKCNKVSGGXXXXXXXXXXXTPRKSWIFDMDENNICIKNNLDNTISDCETMVDVSILQHDEEKMRGKWQIDDEITSDTVVLQNIKLNQDSGGSTIVLDQPSIEIDVPRISESMNCNENSIMEISNLSTIIEVSQEIIEITLNDDKKIKSNFFEREEVWNQNVSKGNIKNQHQPICTLENKKEKSENEALEKVKNTQIIGLEXXXXXXXXXXXVSETAENSDIIDSSGSDTEDLIECFKNAXXXXXXXXXXXXXXXXXXXXTINIGIFNDQSKISNANSLQNLTEKPVTRIRRTKSYSEFEMNLNKEWSSEDTFSSEDFGDDEISNPGNIFLKIEKIRAELEEKIGIDSLMDAVEFVRENQDLNGDQLAEKLKDMIGDEASINDILQLVVLEEQYHNIE 3478
MSSISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNETKKQIQKRPSTASVVSRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHDKRVSPRIVKQDANPLVVRQQELNNKYKVEMINVDRENGWKNVLSSDDSKSDCKESKLNAKLNVYGMTPAAIKQKINSPAYDRYYAELDRIKEQQVLRENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDASKPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQSPIPRQTPRSIEEETTERLNKIRQKNFEIRQGMGFEDFVKNSDIDGNGNFENFNQLEARKALEDLKKRVKQKTEELKQKLERKEKERNKELPSVIDDDPPNNAILFPKRPPVVVNKPENSSNVSHPIAEPGIISVILERIHENITPAGDAPKVDNIELPESLSVILSQLDDKSRPSTASCSDQSEIRKRKDDILKRLNMKCNKVSGGEKENEEAKEKETPRKSWIFDMDENNICIKNNLDNTISDCETMVDVSILQHDEEKMRGKWQIDDEITSDTVVLQNIKLNQDSGGSTIVLDQPSIEIDVPRISESMNCNENSIMEISNLSTIIEVSQEIIEITLNDDKKIKSNFFEREEVWNQNVSKGNIKNQHQPICTLENKKEKSENEALEKVKNTQIIGLEDDIIITNIDDDVSETAENSDIIDSSGSDTEDLIECFKNALSIDISNSSSSKSVDNLSDKTINIGIFNDQSKISNANSLQNLTEKPVTRIRRTKSYSEFEMNLNKEWSSEDTFSSEDFGDDEISNPGNIFLKIEKIRAELEEKIGIDSLMDAVEFVRENQDLNGDQLAEKLKDMIGDEASINDILQLVVLEEQYHNIE
Sbjct: 1 MSSISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNETKKQIQKRPSTASVVSRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHDKRVSPRIVKQDANPLVVRQQELNNKYKVEMINVDRENGWKNVLSSDDSKSDCKESKLNAKLNVYGMTPAAIKQKINSPAYDRYYAELDRIKEQQVLRENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDASKPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQSPIPRQTPRSIEEETTERLNKIRQKNFEIRQGMGFEDFVKNSDIDGNGNFENFNQLEARKALEDLKKRVKQKTEELKQKLERKEKERNKELPSVIDDDPPNNAILFPKRPPVVVNKPENSSNVSHPIAEPGIISVILERIHENITPAGDAPKVDNIELPESLSVILSQLDDKSRPSTASCSDQSEIRKRKDDILKRLNMKCNKVSGGEKENEEAKEKETPRKSWIFDMDENNICIKNNLDNTISDCETMVDVSILQHDEEKMRGKWQIDDEITSDTVVLQNIKLNQDSGGSTIVLDQPSIEIDVPRISESMNCNENSIMEISNLSTIIEVSQEIIEITLNDDKKIKSNFFEREEVWNQNVSKGNIKNQHQPICTLENKKEKSENEALEKVKNTQIIGLEDDIIITNIDDDVSETAENSDIIDSSGSDTEDLIECFKNALSIDISNSSSSKSVDNLSDKTINIGIFNDQSKISNANSLQNLTEKPVTRIRRTKSYSEFEMNLNKEWSSEDTFSSEDFGDDEISNPGNIFLKIEKIRAELEEKIGIDSLMDAVEFVRENQDLNGDQLAEKLKDMIGDEASINDILQLVVLEEQYHNIE 1124
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000020466.1 (SMESG000020466.1) HSP 1 Score: 2118.58 bits (5488), Expect = 0.000e+0 Identity = 1124/1125 (99.91%), Postives = 1124/1125 (99.91%), Query Frame = 2
Query: 107 MSSISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNETKKQIQKRPSTASVVSRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHDKRVSPRIVKQDANPLVVRQQELNNKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPAAIKQKINSPAYDRYYAELDRIKEQQVLRENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDASKPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQSPIPRQTPRSIEEETTERLNKIRQKNFEIRQGMGFEDFVKNSDIDGNGNFENFNQLEARKALEDLKKRVXXXXXXXXXXXXXXXXXXXXXLPSVIDDDPPNNAILFPKR-PPVVVNKPENSSNVSHPIAEPGIISVILERIHENITPAGDAPKVDNIELPESLSVILSQLDDKSRPSTASCSDQSEIRKRKDDILKRLNMKCNKVSGGXXXXXXXXXXXTPRKSWIFDMDENNICIKNNLDNTISDCETMVDVSILQHDEEKMRGKWQIDDEITSDTVVLQNIKLNQDSGGSTIVLDQPSIEIDVPRISESMNCNENSIMEISNLSTIIEVSQEIIEITLNDDKKIKSNFFEREEVWNQNVSKGNIKNQHQPICTLENKKEKSENEALEKVKNTQIIGLEXXXXXXXXXXXVSETAENSDIIDSSGSDTEDLIECFKNAXXXXXXXXXXXXXXXXXXXXTINIGIFNDQSKISNANSLQNLTEKPVTRIRRTKSYSEFEMNLNKEWSSEDTFSSEDFGDDEISNPGNIFLKIEKIRAELEEKIGIDSLMDAVEFVRENQDLNGDQLAEKLKDMIGDEASINDILQLVVLEEQYHNIE 3478
MSSISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNETKKQIQKRPSTASVVSRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHDKRVSPRIVKQDANPLVVRQQELNNKYKVEMINVDRENGWKNVLSSDDSKSDCKESKLNAKLNVYGMTPAAIKQKINSPAYDRYYAELDRIKEQQVLRENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDASKPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQSPIPRQTPRSIEEETTERLNKIRQKNFEIRQGMGFEDFVKNSDIDGNGNFENFNQLEARKALEDLKKRVKQKTEELKQKLERKEKERNKELPSVIDDDPPNNAILFPKR PPVVVNKPENSSNVSHPIAEPGIISVILERIHENITPAGDAPKVDNIELPESLSVILSQLDDKSRPSTASCSDQSEIRKRKDDILKRLNMKCNKVSGGEKENEEAKEKETPRKSWIFDMDENNICIKNNLDNTISDCETMVDVSILQHDEEKMRGKWQIDDEITSDTVVLQNIKLNQDSGGSTIVLDQPSIEIDVPRISESMNCNENSIMEISNLSTIIEVSQEIIEITLNDDKKIKSNFFEREEVWNQNVSKGNIKNQHQPICTLENKKEKSENEALEKVKNTQIIGLEDDIIITNIDDDVSETAENSDIIDSSGSDTEDLIECFKNALSIDISNSSSSKSVDNLSDKTINIGIFNDQSKISNANSLQNLTEKPVTRIRRTKSYSEFEMNLNKEWSSEDTFSSEDFGDDEISNPGNIFLKIEKIRAELEEKIGIDSLMDAVEFVRENQDLNGDQLAEKLKDMIGDEASINDILQLVVLEEQYHNIE
Sbjct: 1 MSSISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNETKKQIQKRPSTASVVSRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHDKRVSPRIVKQDANPLVVRQQELNNKYKVEMINVDRENGWKNVLSSDDSKSDCKESKLNAKLNVYGMTPAAIKQKINSPAYDRYYAELDRIKEQQVLRENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDASKPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQSPIPRQTPRSIEEETTERLNKIRQKNFEIRQGMGFEDFVKNSDIDGNGNFENFNQLEARKALEDLKKRVKQKTEELKQKLERKEKERNKELPSVIDDDPPNNAILFPKRSPPVVVNKPENSSNVSHPIAEPGIISVILERIHENITPAGDAPKVDNIELPESLSVILSQLDDKSRPSTASCSDQSEIRKRKDDILKRLNMKCNKVSGGEKENEEAKEKETPRKSWIFDMDENNICIKNNLDNTISDCETMVDVSILQHDEEKMRGKWQIDDEITSDTVVLQNIKLNQDSGGSTIVLDQPSIEIDVPRISESMNCNENSIMEISNLSTIIEVSQEIIEITLNDDKKIKSNFFEREEVWNQNVSKGNIKNQHQPICTLENKKEKSENEALEKVKNTQIIGLEDDIIITNIDDDVSETAENSDIIDSSGSDTEDLIECFKNALSIDISNSSSSKSVDNLSDKTINIGIFNDQSKISNANSLQNLTEKPVTRIRRTKSYSEFEMNLNKEWSSEDTFSSEDFGDDEISNPGNIFLKIEKIRAELEEKIGIDSLMDAVEFVRENQDLNGDQLAEKLKDMIGDEASINDILQLVVLEEQYHNIE 1125
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000020466.1 (SMESG000020466.1) HSP 1 Score: 2070.43 bits (5363), Expect = 0.000e+0 Identity = 1106/1125 (98.31%), Postives = 1106/1125 (98.31%), Query Frame = 2
Query: 107 MSSISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNETKKQIQKRPSTASVVSRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHDKRVSPRIVKQDANPLVVRQQELNNKYKVEMINVDRENGWKNVLXXXXXXXXXXXXXLNAKLNVYGMTPAAIKQKINSPAYDRYYAELDRIKEQQVLRENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDASKPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQSPIPRQTPRSIEEETTERLNKIRQKNFEIRQGMGFEDFVKNSDIDGNGNFENFNQLEARKALEDLKKRVXXXXXXXXXXXXXXXXXXXXXLPSVIDDDPPNNAILFPKR-PPVVVNKPENSSNVSHPIAEPGIISVILERIHENITPAGDAPKVDNIELPESLSVILSQLDDKSRPSTASCSDQSEIRKRKDDILKRLNMKCNKVSGGXXXXXXXXXXXTPRKSWIFDMDENNICIKNNLDNTISDCETMVDVSILQHDEEKMRGKWQIDDEITSDTVVLQNIKLNQDSGGSTIVLDQPSIEIDVPRISESMNCNENSIMEISNLSTIIEVSQEIIEITLNDDKKIKSNFFEREEVWNQNVSKGNIKNQHQPICTLENKKEKSENEALEKVKNTQIIGLEXXXXXXXXXXXVSETAENSDIIDSSGSDTEDLIECFKNAXXXXXXXXXXXXXXXXXXXXTINIGIFNDQSKISNANSLQNLTEKPVTRIRRTKSYSEFEMNLNKEWSSEDTFSSEDFGDDEISNPGNIFLKIEKIRAELEEKIGIDSLMDAVEFVRENQDLNGDQLAEKLKDMIGDEASINDILQLVVLEEQYHNIE 3478
MSSISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNETKKQIQKRPSTASVVSRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHDKRVSPRIVKQDANPLVVRQQELNNKYKVEMINVDRENGWKNVLSSDDSKSDCKESKLNAKLNVYGMTPAAIKQKINSPAYDRYYAELDRIKEQQVLRENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDASKPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQSPIPRQTPRSIEEETTERLNKIRQKNFEIRQGMGFEDFVKNSDIDGNGNFENFNQLEARKALEDLKKRVKQKTEELKQKLERKEKERNKELPSVIDDDPPNNAILFPKR PPVVVNKPENSSNVSHPIAEPGIISVILERIHENITPAGDAPKVDNIELPESLSVILSQLDDKSRPSTASCSDQSEIRKRKDDILKRLNMKCNKVSGGEKENEEAKEKETPRKSWIFDMDENNI VSILQHDEEKMRGKWQIDDEITSDTVVLQNIKLNQDSGGSTIVLDQPSIEIDVPRISESMNCNENSIMEISNLSTIIEVSQEIIEITLNDDKKIKSNFFEREEVWNQNVSKGNIKNQHQPICTLENKKEKSENEALEKVKNTQIIGLEDDIIITNIDDDVSETAENSDIIDSSGSDTEDLIECFKNALSIDISNSSSSKSVDNLSDKTINIGIFNDQSKISNANSLQNLTEKPVTRIRRTKSYSEFEMNLNKEWSSEDTFSSEDFGDDEISNPGNIFLKIEKIRAELEEKIGIDSLMDAVEFVRENQDLNGDQLAEKLKDMIGDEASINDILQLVVLEEQYHNIE
Sbjct: 1 MSSISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFLPPENPLLNETKKQIQKRPSTASVVSRVDSGLSNKKKARVGRDNALINSPYMQIPNRNIHDKRVSPRIVKQDANPLVVRQQELNNKYKVEMINVDRENGWKNVLSSDDSKSDCKESKLNAKLNVYGMTPAAIKQKINSPAYDRYYAELDRIKEQQVLRENEGRALMQHMLKQNDNKSPDGKLLKVGADGLYRKIDASKPYDERSKMNELARIHGKIGGNAADQAVIQEFIERKRQAKNIKNRNGSGMALLMGGHQFCAEQSPIPRQTPRSIEEETTERLNKIRQKNFEIRQGMGFEDFVKNSDIDGNGNFENFNQLEARKALEDLKKRVKQKTEELKQKLERKEKERNKELPSVIDDDPPNNAILFPKRSPPVVVNKPENSSNVSHPIAEPGIISVILERIHENITPAGDAPKVDNIELPESLSVILSQLDDKSRPSTASCSDQSEIRKRKDDILKRLNMKCNKVSGGEKENEEAKEKETPRKSWIFDMDENNIY-----------------VSILQHDEEKMRGKWQIDDEITSDTVVLQNIKLNQDSGGSTIVLDQPSIEIDVPRISESMNCNENSIMEISNLSTIIEVSQEIIEITLNDDKKIKSNFFEREEVWNQNVSKGNIKNQHQPICTLENKKEKSENEALEKVKNTQIIGLEDDIIITNIDDDVSETAENSDIIDSSGSDTEDLIECFKNALSIDISNSSSSKSVDNLSDKTINIGIFNDQSKISNANSLQNLTEKPVTRIRRTKSYSEFEMNLNKEWSSEDTFSSEDFGDDEISNPGNIFLKIEKIRAELEEKIGIDSLMDAVEFVRENQDLNGDQLAEKLKDMIGDEASINDILQLVVLEEQYHNIE 1108
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000005635.1 (SMESG000005635.1) HSP 1 Score: 239.195 bits (609), Expect = 6.639e-67 Identity = 118/261 (45.21%), Postives = 169/261 (64.75%), Query Frame = 2
Query: 134 LNKIGQGAFGIAWLVESK-IDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRKPFILKKIDKFL 913
+ KIGQG++G WLV+ K D KK+VLK+I + ++E AR E LL + HPNIV Y +SFEE +I M +CE GDLY ++K + G L E Q++++ IQI +AL +IH++ ILHRD+K QNIFLTK + +K+GD GIAK ++S + KT +GTP+Y SPE+ E PYN KSDIWSLGC+ YE+ +L +AF ++ T L+ +I +PS ++ L L+ M + P++RP+ IL FI I F+
Sbjct: 11 IKKIGQGSYGEVWLVKHKDYDHKKFVLKKISFARSSDREMEAARLECQLLKSLEHPNIVQYKDSFEENNSLYIAMAFCESGDLYTRLKERKGSLLCEKQIIEWLIQITMALSYIHERNILHRDLKTQNIFLTK-SIIKIGDLGIAKQLESKYDMAKTVIGTPYYMSPELFEKKPYNFKSDIWSLGCVTYEMATLKHAFNAKNYTALSYKILMGQTPDMPSKYNKELINLIQAMLNKSPEKRPSTGKILCNNFIRDHIHMFV 270
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000043302.1 (SMESG000043302.1) HSP 1 Score: 222.631 bits (566), Expect = 1.164e-61 Identity = 116/311 (37.30%), Postives = 181/311 (58.20%), Query Frame = 2
Query: 104 VMSSISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEARKEVSLLSKMNHPNIVTYYNSFEEVGCFHILMEYCEGGDLYRKIKTQNGVLFKEDQVLDYFIQICLALKHIHDQRILHRDIKPQNIFLTKNNSVKLGDFGIAKAMDSTTALTKTQVGTPFYFSPEICEGCPYNNKSDIWSLGCILYELCSLNYAFYGQDITILAMRIKKVNYKPVPSVFSAPLRALVDNMFRRRPDERPNINTILRK--PFILKKIDKFLPPENPLLNETKKQIQKRPSTASVVSRVDSGLSNKKKARV 1030
+ +I+ F+++ +G+GA+G A L K D +LKEI ++ + ER A EV +LS+++HPNIV Y++SFEE G I MEY + G L + + Q L +E ++L+ F QI A+ +IH+ +ILHRD+K NIFLTK +K+GDFGI+K + +T + +GTP+Y SPE+C+G YN KSD+W+LGCILYE+ L+ F G ++ L +I K ++PV +S L+ L+ ++ + PD+R + IL P ++K + + +P T SVV +D N R+
Sbjct: 53 IQQTINGFEKIRTVGKGAYGTAVLYRKKDDDSLVILKEINMHDLSATERQLAMNEVKVLSQLDHPNIVAYFDSFEEDGVLMIEMEYADNGTLAQFLSNQEKPL-EEKKILEMFFQIVSAIHYIHEHKILHRDLKTVNIFLTKEEIIKIGDFGISKMLSTTHEGANSVIGTPYYISPEMCQGKLYNEKSDVWALGCILYEMTCLHKTFEGSNLPALVNKILKGQFEPVKGSYSDELKELISSLLEKNPDDRLSTEVILHHKLPMMMKTFHT---------DSSDNVDTAKPQTRSVVYVMDINTLNLSPVRL 353 The following BLAST results are available for this feature:
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30013054 ID=SMED30013054|Name=Protein kinase domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=3643bpback to top protein sequence of SMED30013054-orf-1 >SMED30013054-orf-1 ID=SMED30013054-orf-1|Name=SMED30013054-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1125bp MSSISDFKRLNKIGQGAFGIAWLVESKIDCKKYVLKEIGLYKMHEKERFEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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