SMED30013041

Overview
NameSMED30013041
Smed IDSMED30013041
Length (bp)2768
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30013041 (SMED30013041) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30013041 (SMED30013041) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30013041 (SMED30013041) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30013041 (SMED30013041) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30013041

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30013041h1SMcG0009137 Contig49723newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013041h1SMcG0009137 Contig49723uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30013041h1SMcG0009137 dd_Smed_v4_4914_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30013041h1SMcG0009137 dd_Smed_v4_4914_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30013041h1SMcG0009137 dd_Smed_v4_4914_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30013041h1SMcG0009137 dd_Smed_v4_4914_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30013041h1SMcG0009137 dd_Smed_v4_4914_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30013041h1SMcG0009137 dd_Smed_v4_4914_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30013041h1SMcG0009137 dd_Smed_v6_4914_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30013041h1SMcG0009137 dd_Smed_v4_4914_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Category 4 cellSMED30013041h1SMcG0009137 dd_Smed_v4_4914_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30013041h1SMcG0009137 dd_Smed_v4_4914_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30013041 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000071:109718..116517 +13607
Homology
BLAST of SMED30013041 vs. Planmine SMEST
Match: SMESG000049878.1 (SMESG000049878.1)

HSP 1 Score: 1388.25 bits (3592), Expect = 0.000e+0
Identity = 765/765 (100.00%), Postives = 765/765 (100.00%), Query Frame = 3
Query:  372 GSIFLNKKSSFRTKINEYCHYKLENLTPTDDYRSLPNRKRTTMNVTFNAYKNEVDPYVHALNWLPRFKARCKGHLARKNAEELKKKHEAVRIIQNAAEEFLDPLMQIFYTIRPTLKILKIDELKKYLRECIIQIKKLNAERMFIENQLDDVEGTLDQSLNINTNNNCLSLEGRKSPNIFGKMSEIKANANNDTFLKECLDLKIDGLENLEVNFDPASCQNSKIHAVYEKSDKLQLKIHENQHKIKDLTNKCNNLEKEIKDLRYNNTTLDSTGRIMKGIIHKNDREKDELINKMADYEDKVKSXXXXXXXXXXXXXXXXXDMSXXXXXXXXXXXXCEYLDNEVDKKTKAVIALNQEKMISDKKIKELEETISYNNEIQQENESNYELQKTRMKDMKKRYLEQIKTSEEDHLSEKNGLKFHLQDLTEQNESLQEKIKQCELTIWRLEREIADSKDRDGSDEDIDHEQERKLSMLLKNAKADSDALKEEMKIIENNYEETKKKNHELESNLDAITIQATEYRKQIERLKAQILTLKSTFSETELNLNRANREKNYTNQKYLQSKEQNVQXXXXXXXXXXXVTTQSQNVSIKLKEIENLESDIHSLRREYESKMSVVQELNYDLQSQITAANEKHNQTQEEMKRITEALRQEIDEQKQMLEEKEIEQENTLMENLKHQQTIKKLQKRISEQDDIQNDSEKTIMXXXXXXXXXXXXXXXXIVTSPTEYRTLDYLRTENKLLRXXXXXXXXXXXRDNTGISTLPRMGSNRY 2666
            GSIFLNKKSSFRTKINEYCHYKLENLTPTDDYRSLPNRKRTTMNVTFNAYKNEVDPYVHALNWLPRFKARCKGHLARKNAEELKKKHEAVRIIQNAAEEFLDPLMQIFYTIRPTLKILKIDELKKYLRECIIQIKKLNAERMFIENQLDDVEGTLDQSLNINTNNNCLSLEGRKSPNIFGKMSEIKANANNDTFLKECLDLKIDGLENLEVNFDPASCQNSKIHAVYEKSDKLQLKIHENQHKIKDLTNKCNNLEKEIKDLRYNNTTLDSTGRIMKGIIHKNDREKDELINKMADYEDKVKSLIEENKKLLNKLKETETDMSKKTETLEELKTKCEYLDNEVDKKTKAVIALNQEKMISDKKIKELEETISYNNEIQQENESNYELQKTRMKDMKKRYLEQIKTSEEDHLSEKNGLKFHLQDLTEQNESLQEKIKQCELTIWRLEREIADSKDRDGSDEDIDHEQERKLSMLLKNAKADSDALKEEMKIIENNYEETKKKNHELESNLDAITIQATEYRKQIERLKAQILTLKSTFSETELNLNRANREKNYTNQKYLQSKEQNVQLEEEILKLKEEVTTQSQNVSIKLKEIENLESDIHSLRREYESKMSVVQELNYDLQSQITAANEKHNQTQEEMKRITEALRQEIDEQKQMLEEKEIEQENTLMENLKHQQTIKKLQKRISEQDDIQNDSEKTIMKLKDENKNLKEERDNEIVTSPTEYRTLDYLRTENKLLRSSSSANSTINSRDNTGISTLPRMGSNRY
Sbjct:   18 GSIFLNKKSSFRTKINEYCHYKLENLTPTDDYRSLPNRKRTTMNVTFNAYKNEVDPYVHALNWLPRFKARCKGHLARKNAEELKKKHEAVRIIQNAAEEFLDPLMQIFYTIRPTLKILKIDELKKYLRECIIQIKKLNAERMFIENQLDDVEGTLDQSLNINTNNNCLSLEGRKSPNIFGKMSEIKANANNDTFLKECLDLKIDGLENLEVNFDPASCQNSKIHAVYEKSDKLQLKIHENQHKIKDLTNKCNNLEKEIKDLRYNNTTLDSTGRIMKGIIHKNDREKDELINKMADYEDKVKSLIEENKKLLNKLKETETDMSKKTETLEELKTKCEYLDNEVDKKTKAVIALNQEKMISDKKIKELEETISYNNEIQQENESNYELQKTRMKDMKKRYLEQIKTSEEDHLSEKNGLKFHLQDLTEQNESLQEKIKQCELTIWRLEREIADSKDRDGSDEDIDHEQERKLSMLLKNAKADSDALKEEMKIIENNYEETKKKNHELESNLDAITIQATEYRKQIERLKAQILTLKSTFSETELNLNRANREKNYTNQKYLQSKEQNVQLEEEILKLKEEVTTQSQNVSIKLKEIENLESDIHSLRREYESKMSVVQELNYDLQSQITAANEKHNQTQEEMKRITEALRQEIDEQKQMLEEKEIEQENTLMENLKHQQTIKKLQKRISEQDDIQNDSEKTIMKLKDENKNLKEERDNEIVTSPTEYRTLDYLRTENKLLRSSSSANSTINSRDNTGISTLPRMGSNRY 782          
BLAST of SMED30013041 vs. Planmine SMEST
Match: SMESG000049878.1 (SMESG000049878.1)

HSP 1 Score: 1386.32 bits (3587), Expect = 0.000e+0
Identity = 765/765 (100.00%), Postives = 765/765 (100.00%), Query Frame = 3
Query:  372 GSIFLNKKSSFRTKINEYCHYKLENLTPTDDYRSLPNRKRTTMNVTFNAYKNEVDPYVHALNWLPRFKARCKGHLARKNAEELKKKHEAVRIIQNAAEEFLDPLMQIFYTIRPTLKILKIDELKKYLRECIIQIKKLNAERMFIENQLDDVEGTLDQSLNINTNNNCLSLEGRKSPNIFGKMSEIKANANNDTFLKECLDLKIDGLENLEVNFDPASCQNSKIHAVYEKSDKLQLKIHENQHKIKDLTNKCNNLEKEIKDLRYNNTTLDSTGRIMKGIIHKNDREKDELINKMADYEDKVKSXXXXXXXXXXXXXXXXXDMSXXXXXXXXXXXXCEYLDNEVDKKTKAVIALNQEKMISDKKIKELEETISYNNEIQQENESNYELQKTRMKDMKKRYLEQIKTSEEDHLSEKNGLKFHLQDLTEQNESLQEKIKQCELTIWRLEREIADSKDRDGSDEDIDHEQERKLSMLLKNAKADSDALKEEMKIIENNYEETKKKNHELESNLDAITIQATEYRKQIERLKAQILTLKSTFSETELNLNRANREKNYTNQKYLQSKEQNVQXXXXXXXXXXXVTTQSQNVSIKLKEIENLESDIHSLRREYESKMSVVQELNYDLQSQITAANEKHNQTQEEMKRITEALRQEIDEQKQMLEEKEIEQENTLMENLKHQQTIKKLQKRISEQDDIQNDSEKTIMXXXXXXXXXXXXXXXXIVTSPTEYRTLDYLRTENKLLRXXXXXXXXXXXRDNTGISTLPRMGSNRY 2666
            GSIFLNKKSSFRTKINEYCHYKLENLTPTDDYRSLPNRKRTTMNVTFNAYKNEVDPYVHALNWLPRFKARCKGHLARKNAEELKKKHEAVRIIQNAAEEFLDPLMQIFYTIRPTLKILKIDELKKYLRECIIQIKKLNAERMFIENQLDDVEGTLDQSLNINTNNNCLSLEGRKSPNIFGKMSEIKANANNDTFLKECLDLKIDGLENLEVNFDPASCQNSKIHAVYEKSDKLQLKIHENQHKIKDLTNKCNNLEKEIKDLRYNNTTLDSTGRIMKGIIHKNDREKDELINKMADYEDKVKSLIEENKKLLNKLKETETDMSKKTETLEELKTKCEYLDNEVDKKTKAVIALNQEKMISDKKIKELEETISYNNEIQQENESNYELQKTRMKDMKKRYLEQIKTSEEDHLSEKNGLKFHLQDLTEQNESLQEKIKQCELTIWRLEREIADSKDRDGSDEDIDHEQERKLSMLLKNAKADSDALKEEMKIIENNYEETKKKNHELESNLDAITIQATEYRKQIERLKAQILTLKSTFSETELNLNRANREKNYTNQKYLQSKEQNVQLEEEILKLKEEVTTQSQNVSIKLKEIENLESDIHSLRREYESKMSVVQELNYDLQSQITAANEKHNQTQEEMKRITEALRQEIDEQKQMLEEKEIEQENTLMENLKHQQTIKKLQKRISEQDDIQNDSEKTIMKLKDENKNLKEERDNEIVTSPTEYRTLDYLRTENKLLRSSSSANSTINSRDNTGISTLPRMGSNRY
Sbjct:   23 GSIFLNKKSSFRTKINEYCHYKLENLTPTDDYRSLPNRKRTTMNVTFNAYKNEVDPYVHALNWLPRFKARCKGHLARKNAEELKKKHEAVRIIQNAAEEFLDPLMQIFYTIRPTLKILKIDELKKYLRECIIQIKKLNAERMFIENQLDDVEGTLDQSLNINTNNNCLSLEGRKSPNIFGKMSEIKANANNDTFLKECLDLKIDGLENLEVNFDPASCQNSKIHAVYEKSDKLQLKIHENQHKIKDLTNKCNNLEKEIKDLRYNNTTLDSTGRIMKGIIHKNDREKDELINKMADYEDKVKSLIEENKKLLNKLKETETDMSKKTETLEELKTKCEYLDNEVDKKTKAVIALNQEKMISDKKIKELEETISYNNEIQQENESNYELQKTRMKDMKKRYLEQIKTSEEDHLSEKNGLKFHLQDLTEQNESLQEKIKQCELTIWRLEREIADSKDRDGSDEDIDHEQERKLSMLLKNAKADSDALKEEMKIIENNYEETKKKNHELESNLDAITIQATEYRKQIERLKAQILTLKSTFSETELNLNRANREKNYTNQKYLQSKEQNVQLEEEILKLKEEVTTQSQNVSIKLKEIENLESDIHSLRREYESKMSVVQELNYDLQSQITAANEKHNQTQEEMKRITEALRQEIDEQKQMLEEKEIEQENTLMENLKHQQTIKKLQKRISEQDDIQNDSEKTIMKLKDENKNLKEERDNEIVTSPTEYRTLDYLRTENKLLRSSSSANSTINSRDNTGISTLPRMGSNRY 787          
BLAST of SMED30013041 vs. Planmine SMEST
Match: SMESG000006536.1 (SMESG000006536.1)

HSP 1 Score: 54.6842 bits (130), Expect = 4.469e-7
Identity = 35/107 (32.71%), Postives = 61/107 (57.01%), Query Frame = 3
Query:  549 ALNWLPRFKARCKGHLARKNAEELKKKHEAVRIIQNAAEEFLD----PLMQIFYTIRPTLKILKIDELKKYLRECIIQIKK----LNAERMF-------IENQLDDV 824
            +LN++ + ++ C+  LA KN E+LK + EA+RIIQ  A  +L     P  ++F  IRP + +L++DE  K  +E + +I+     +  ER +       +EN +DD+
Sbjct: 1067 SLNFIIKLQSLCRRFLAHKNFEKLKLQTEAIRIIQRNALMYLKIKEWPWWKLFSRIRPLMSVLRVDEELKITKEKLNEIQSRSIAIETERDYFKQENRKLENSIDDL 1173          
The following BLAST results are available for this feature:
BLAST of SMED30013041 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013041 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013041 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013041 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013041 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013041 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013041 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013041 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013041 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013041 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013041 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013041 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013041 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30013041 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000049878.10.000e+0100.00SMESG000049878.1[more]
SMESG000049878.10.000e+0100.00SMESG000049878.1[more]
SMESG000006536.14.469e-732.71SMESG000006536.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013041 ID=SMED30013041|Name=SMED30013041|organism=Schmidtea mediterranea sexual|type=transcript|length=2768bp
CAATAGATTTGCAGTGCTATTGTTAAATGGCACAAGAGGCCGCCACTGGC
ACACGCGACTTGATCAAGTAGTCAATGAATCTGAATCAAACACAACGACA
AGCACACATAGCACATCACAATAAATGATCCACTGATCAAGTGTGCTCTG
ATCGCACAACTGTTGCTGTGTGCTGATCAATGAGATCGATCGACAGAAAG
TGATAGTGCATCAAGCACACTGCTCAGTTCTTTAACGCATTGATTAACTC
ATTAAAATTTATTTTCAGATTTCGGTTTTTCTTTTTTTCACAATTTATTG
TTTATATGTGTACACCTAACATGCCACCAACTCCGACTTTGACATCAACT
CTGACAAACTTGTCGACTCCGGGATCTATTTTCCTCAACAAAAAATCGAG
TTTTCGAACAAAAATCAACGAGTACTGCCATTACAAGCTGGAAAATCTAA
CCCCAACAGATGACTACCGCTCTTTGCCGAACAGGAAGCGGACGACAATG
AATGTGACGTTTAATGCATACAAAAATGAAGTTGATCCCTACGTCCATGC
TCTCAACTGGCTGCCGAGATTCAAGGCCCGTTGTAAAGGACATCTTGCAA
GAAAGAATGCCGAAGAACTCAAGAAAAAACACGAAGCGGTGAGGATCATC
CAAAACGCAGCCGAGGAATTCCTGGATCCTTTGATGCAAATATTCTACAC
GATTCGGCCAACTCTAAAAATTTTAAAAATCGACGAATTAAAAAAATATT
TGAGAGAATGCATCATCCAAATCAAGAAACTGAACGCCGAAAGAATGTTT
ATCGAAAACCAACTTGATGATGTGGAGGGTACGTTGGATCAGTCATTGAA
TATCAACACGAATAACAACTGCCTTTCACTGGAAGGAAGGAAATCTCCAA
ACATTTTCGGGAAAATGTCCGAGATCAAGGCAAACGCCAACAACGATACT
TTCCTCAAAGAATGTCTTGATTTGAAAATCGACGGCTTGGAAAACTTAGA
GGTAAACTTTGATCCAGCAAGTTGCCAAAATAGCAAGATTCACGCAGTTT
ATGAAAAGTCTGACAAGTTGCAACTGAAAATCCACGAAAACCAACATAAG
ATAAAAGACCTGACGAATAAATGCAACAATTTGGAGAAGGAAATCAAAGA
TCTAAGATATAACAATACAACTCTTGACTCTACCGGGAGAATAATGAAGG
GAATTATTCACAAGAACGACAGAGAAAAGGACGAACTCATCAATAAGATG
GCTGATTATGAAGACAAAGTTAAAAGTTTGATTGAAGAAAACAAGAAACT
TTTAAACAAATTAAAGGAAACCGAAACCGATATGTCTAAAAAAACTGAAA
CTCTCGAAGAATTGAAAACAAAATGCGAATATCTGGACAATGAAGTCGAC
AAGAAAACCAAAGCTGTCATTGCACTGAACCAGGAGAAGATGATTTCAGA
CAAAAAGATTAAAGAGCTTGAAGAAACGATCAGCTATAACAATGAAATTC
AGCAGGAAAACGAATCGAACTATGAGCTGCAAAAAACTCGAATGAAAGAT
ATGAAAAAACGATATCTAGAGCAAATCAAAACGTCCGAAGAAGACCATTT
GTCAGAAAAGAACGGCTTGAAATTTCATCTGCAAGATTTGACAGAACAAA
ACGAATCACTACAAGAGAAAATCAAACAATGCGAATTAACAATCTGGAGA
TTAGAAAGAGAAATTGCCGACTCAAAAGACAGAGATGGAAGCGATGAAGA
TATTGATCATGAACAAGAACGAAAGTTGAGTATGTTGTTGAAAAATGCAA
AAGCAGATAGCGACGCGCTGAAAGAAGAAATGAAAATAATCGAGAACAAT
TACGAAGAAACAAAAAAGAAAAATCACGAACTGGAAAGCAATCTAGACGC
AATAACCATACAAGCAACGGAATACAGAAAGCAAATAGAACGATTGAAAG
CTCAGATTTTGACTTTGAAGTCCACTTTTAGTGAAACGGAATTAAATCTG
AATAGAGCCAACAGAGAAAAAAATTACACAAACCAAAAGTATTTACAATC
CAAAGAACAAAATGTGCAACTGGAAGAGGAGATATTGAAACTTAAAGAAG
AAGTTACGACGCAATCGCAAAATGTGAGTATAAAGTTAAAAGAAATAGAA
AATCTCGAAAGCGACATTCACAGCTTGAGGAGAGAGTATGAATCCAAAAT
GTCAGTGGTTCAAGAATTGAATTACGATCTGCAATCCCAAATCACTGCCG
CAAATGAGAAGCACAATCAAACTCAAGAAGAAATGAAAAGAATTACAGAA
GCGCTAAGACAAGAAATTGATGAACAAAAACAAATGCTCGAGGAGAAAGA
GATAGAACAAGAAAATACTCTGATGGAGAACTTGAAACATCAGCAGACCA
TTAAAAAACTGCAAAAAAGAATTTCCGAACAAGATGATATCCAAAATGAT
TCCGAGAAAACGATTATGAAATTAAAAGATGAAAATAAAAATCTTAAAGA
GGAGAGAGATAATGAAATTGTCACCTCTCCGACAGAATATCGCACCTTGG
ACTACCTACGAACAGAGAACAAACTGCTCAGGTCCTCCAGCTCCGCCAAT
TCCACCATAAATTCCAGAGACAATACCGGGATCTCGACACTGCCAAGAAT
GGGGAGCAACAGATATTAATGGAATAAATCAACTGAAAGTTGTCTATTAA
AATATTATTATGAATTTTAGTAATTTCTTTTCGTTTGCACTATGAAGAAT
GAATAAATTATTCCTTGT
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protein sequence of SMED30013041-orf-1

>SMED30013041-orf-1 ID=SMED30013041-orf-1|Name=SMED30013041-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=788bp
MCTPNMPPTPTLTSTLTNLSTPGSIFLNKKSSFRTKINEYCHYKLENLTP
TDDYRSLPNRKRTTMNVTFNAYKNEVDPYVHALNWLPRFKARCKGHLARK
NAEELKKKHEAVRIIQNAAEEFLDPLMQIFYTIRPTLKILKIDELKKYLR
ECIIQIKKLNAERMFIENQLDDVEGTLDQSLNINTNNNCLSLEGRKSPNI
FGKMSEIKANANNDTFLKECLDLKIDGLENLEVNFDPASCQNSKIHAVYE
KSDKLQLKIHENQHKIKDLTNKCNNLEKEIKDLRYNNTTLDSTGRIMKGI
IHKNDREKDELINKMADYEDKVKSLIEENKKLLNKLKETETDMSKKTETL
EELKTKCEYLDNEVDKKTKAVIALNQEKMISDKKIKELEETISYNNEIQQ
ENESNYELQKTRMKDMKKRYLEQIKTSEEDHLSEKNGLKFHLQDLTEQNE
SLQEKIKQCELTIWRLEREIADSKDRDGSDEDIDHEQERKLSMLLKNAKA
DSDALKEEMKIIENNYEETKKKNHELESNLDAITIQATEYRKQIERLKAQ
ILTLKSTFSETELNLNRANREKNYTNQKYLQSKEQNVQLEEEILKLKEEV
TTQSQNVSIKLKEIENLESDIHSLRREYESKMSVVQELNYDLQSQITAAN
EKHNQTQEEMKRITEALRQEIDEQKQMLEEKEIEQENTLMENLKHQQTIK
KLQKRISEQDDIQNDSEKTIMKLKDENKNLKEERDNEIVTSPTEYRTLDY
LRTENKLLRSSSSANSTINSRDNTGISTLPRMGSNRY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000018Category 4 cell
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000054dorsal region of the epidermis
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR027401Myosin_IQ_contain_sf
IPR000048IQ_motif_EF-hand-BS
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
Vocabulary: biological process
TermDefinition
GO:0006886intracellular protein transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 252..293
NoneNo IPR availableCOILSCoilCoilcoord: 435..476
NoneNo IPR availableCOILSCoilCoilcoord: 579..634
NoneNo IPR availableCOILSCoilCoilcoord: 639..696
NoneNo IPR availableCOILSCoilCoilcoord: 301..391
NoneNo IPR availableCOILSCoilCoilcoord: 713..740
NoneNo IPR availableCOILSCoilCoilcoord: 488..564
NoneNo IPR availableCOILSCoilCoilcoord: 142..176
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 759..787
NoneNo IPR availablePANTHERPTHR45615FAMILY NOT NAMEDcoord: 86..428
IPR027401Myosin IQ motif-containing domain superfamilyGENE3DG3DSA:4.10.270.10coord: 84..137
e-value: 3.7E-6
score: 28.7
IPR000048IQ motif, EF-hand binding sitePROSITEPS50096IQcoord: 86..111
score: 6.614