40S ribosomal protein S6

Overview
Name40S ribosomal protein S6
Smed IDSMED30013018
Length (bp)811
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 40S ribosomal protein S6 (SMED30013018) t-SNE clustered cells

Violin plots show distribution of expression levels for 40S ribosomal protein S6 (SMED30013018) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 40S ribosomal protein S6 (SMED30013018) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 40S ribosomal protein S6 (SMED30013018) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30013018

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 17

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30013018SMESG000000674.1 SmedASXL_006750SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30013018SMESG000000674.1 dd_Smed_v4_178_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30013018SMESG000000674.1 dd_Smed_v4_178_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30013018SMESG000000674.1 dd_Smed_v4_178_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30013018SMESG000000674.1 dd_Smed_v4_178_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30013018SMESG000000674.1 dd_Smed_v4_178_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30013018SMESG000000674.1 dd_Smed_v4_178_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30013018SMESG000000674.1 dd_Smed_v4_178_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30013018SMESG000000674.1 dd_Smed_v4_178_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30013018SMESG000000674.1 dd_Smed_v4_178_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30013018SMESG000000674.1 dd_Smed_v4_178_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013018SMESG000000674.1 Contig46642newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013018SMESG000000674.1 Contig46642uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013018SMESG000000674.1 Contig33365newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30013018SMESG000000674.1 Contig33365uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
neoblastSMED30013018SMESG000000674.1 dd_Smed_v6_178_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gamma neoblastSMED30013018SMESG000000674.1 dd_Smed_v6_178_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of 40S ribosomal protein S6 vs. Ensembl Human
Match: RPS6 (ribosomal protein S6 [Source:HGNC Symbol;Acc:HGNC:10429])

HSP 1 Score: 242.276 bits (617), Expect = 9.559e-80
Identity = 139/215 (64.65%), Postives = 171/215 (79.53%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            MKLNIS+PA G QK IEVDD  KL+ F +K ++  V+AD+LG+ WKGY+++I+GGNDKQGFPM QGVLT+ RVRLLL KG  CYR RR GERKRKSVRGCIVD NLSV++  I +KG  +IPGLTDT VP+RLGPKRAS+IRKLFNL+ +DDVR+YV+R+ L  K+GKK ++KAPKIQ LVTP  LQ +RR  ALK+QR  K   +  EY K++A
Sbjct:    1 MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPL-NKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLA 214          
BLAST of 40S ribosomal protein S6 vs. Ensembl Human
Match: RPS6 (ribosomal protein S6 [Source:HGNC Symbol;Acc:HGNC:10429])

HSP 1 Score: 202.601 bits (514), Expect = 1.351e-64
Identity = 119/180 (66.11%), Postives = 146/180 (81.11%), Query Frame = 3
Query:  168 VSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            V+AD+LG+ WKGY+++I+GGNDKQGFPM QGVLT+ RVRLLL KG  CYR RR GERKRKSVRGCIVD NLSV++  I +KG  +IPGLTDT VP+RLGPKRAS+IRKLFNL+ +DDVR+YV+R+ L  K+GKK ++KAPKIQ LVTP  LQ +RR  ALK+QR  K   +  EY K++A
Sbjct:    5 VAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPL-NKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLA 183          
BLAST of 40S ribosomal protein S6 vs. Ensembl Human
Match: RPS6 (ribosomal protein S6 [Source:HGNC Symbol;Acc:HGNC:10429])

HSP 1 Score: 202.601 bits (514), Expect = 1.351e-64
Identity = 119/180 (66.11%), Postives = 146/180 (81.11%), Query Frame = 3
Query:  168 VSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            V+AD+LG+ WKGY+++I+GGNDKQGFPM QGVLT+ RVRLLL KG  CYR RR GERKRKSVRGCIVD NLSV++  I +KG  +IPGLTDT VP+RLGPKRAS+IRKLFNL+ +DDVR+YV+R+ L  K+GKK ++KAPKIQ LVTP  LQ +RR  ALK+QR  K   +  EY K++A
Sbjct:    5 VAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPL-NKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLA 183          
BLAST of 40S ribosomal protein S6 vs. Ensembl Human
Match: RPS6 (ribosomal protein S6 [Source:HGNC Symbol;Acc:HGNC:10429])

HSP 1 Score: 59.6918 bits (143), Expect = 3.554e-11
Identity = 27/46 (58.70%), Postives = 35/46 (76.09%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWK 200
            MKLNIS+PA G QK IEVDD  KL+ F +K ++  V+AD+LG+ WK
Sbjct:    1 MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWK 46          
BLAST of 40S ribosomal protein S6 vs. Ensembl Celegans
Match: rps-6 (40S ribosomal protein S6 [Source:UniProtKB/Swiss-Prot;Acc:Q9NEN6])

HSP 1 Score: 224.172 bits (570), Expect = 3.752e-73
Identity = 116/185 (62.70%), Postives = 140/185 (75.68%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRE--LPEXXXXXXXXXXXXIQNLVTP 611
            M+LN +YPA G QK  EVD+  KL+ F +K +S  V+ D+LGD WKGY+++I GGNDKQGFPM QG+LTN RVRLLLKKG  CYRER+ GERKRKSVRGCIVD N+S +S  I +KG  EI GLTD+ +P++LGPKRASKIRKLFNLT  DDV KYVI  +   P+      K+ APKIQ L+TP
Sbjct:    1 MRLNFAYPATGLQKSFEVDEEKKLRLFFEKRMSQEVAIDALGDEWKGYVVRIGGGNDKQGFPMKQGILTNGRVRLLLKKGQSCYRERKNGERKRKSVRGCIVDANMSALSLVIVKKGDGEIEGLTDSVLPRKLGPKRASKIRKLFNLTKHDDVTKYVITHDKTFPD---GVTKTIAPKIQRLITP 182          
BLAST of 40S ribosomal protein S6 vs. Ensembl Celegans
Match: rps-6 (40S ribosomal protein S6 [Source:UniProtKB/Swiss-Prot;Acc:Q9NEN6])

HSP 1 Score: 152.91 bits (385), Expect = 1.179e-45
Identity = 90/185 (48.65%), Postives = 110/185 (59.46%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRE--LPEXXXXXXXXXXXXIQNLVTP 611
            M+LN +YPA G QK  EVD+  KL+ F +K +S  V+ D+LGD WKG                                   CYRER+ GERKRKSVRGCIVD N+S +S  I +KG  EI GLTD+ +P++LGPKRASKIRKLFNLT  DDV KYVI  +   P+      K+ APKIQ L+TP
Sbjct:    1 MRLNFAYPATGLQKSFEVDEEKKLRLFFEKRMSQEVAIDALGDEWKG---------------------------------QSCYRERKNGERKRKSVRGCIVDANMSALSLVIVKKGDGEIEGLTDSVLPRKLGPKRASKIRKLFNLTKHDDVTKYVITHDKTFPD---GVTKTIAPKIQRLITP 149          
BLAST of 40S ribosomal protein S6 vs. Ensembl Celegans
Match: rps-6 (40S ribosomal protein S6 [Source:UniProtKB/Swiss-Prot;Acc:Q9NEN6])

HSP 1 Score: 144.05 bits (362), Expect = 1.222e-42
Identity = 80/123 (65.04%), Postives = 93/123 (75.61%), Query Frame = 3
Query:  249 MMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRE--LPEXXXXXXXXXXXXIQNLVTP 611
            M QG+LTN RVRLLLKKG  CYRER+ GERKRKSVRGCIVD N+S +S  I +KG  EI GLTD+ +P++LGPKRASKIRKLFNLT  DDV KYVI  +   P+      K+ APKIQ L+TP
Sbjct:    1 MKQGILTNGRVRLLLKKGQSCYRERKNGERKRKSVRGCIVDANMSALSLVIVKKGDGEIEGLTDSVLPRKLGPKRASKIRKLFNLTKHDDVTKYVITHDKTFPD---GVTKTIAPKIQRLITP 120          
BLAST of 40S ribosomal protein S6 vs. Ensembl Celegans
Match: rps-6 (40S ribosomal protein S6 [Source:UniProtKB/Swiss-Prot;Acc:Q9NEN6])

HSP 1 Score: 144.05 bits (362), Expect = 1.222e-42
Identity = 80/123 (65.04%), Postives = 93/123 (75.61%), Query Frame = 3
Query:  249 MMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRE--LPEXXXXXXXXXXXXIQNLVTP 611
            M QG+LTN RVRLLLKKG  CYRER+ GERKRKSVRGCIVD N+S +S  I +KG  EI GLTD+ +P++LGPKRASKIRKLFNLT  DDV KYVI  +   P+      K+ APKIQ L+TP
Sbjct:    1 MKQGILTNGRVRLLLKKGQSCYRERKNGERKRKSVRGCIVDANMSALSLVIVKKGDGEIEGLTDSVLPRKLGPKRASKIRKLFNLTKHDDVTKYVITHDKTFPD---GVTKTIAPKIQRLITP 120          
BLAST of 40S ribosomal protein S6 vs. Ensembl Fly
Match: RpS6 (gene:FBgn0261592 transcript:FBtr0071135)

HSP 1 Score: 247.669 bits (631), Expect = 3.750e-82
Identity = 126/185 (68.11%), Postives = 149/185 (80.54%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPIT 617
            MKLN+SYPA G QK  EV D HKL+ F +K +  +V AD LGD WKGY L+I GGNDKQGFPM QGVLT+ RVRLLLKKG  CYR RR GERKRKSVRGCIVD N+SV++  + +KG  +IPGLTDT +P+RLGPKRASKIRKL+NL+ +DDVR++V+RR LP KD KKA SKAPKIQ L+TP+ 
Sbjct:    1 MKLNVSYPATGCQKLFEVVDEHKLRVFYEKRMGQVVEADILGDEWKGYQLRIAGGNDKQGFPMKQGVLTHGRVRLLLKKGHSCYRPRRTGERKRKSVRGCIVDANMSVLALVVLKKGEKDIPGLTDTTIPRRLGPKRASKIRKLYNLSKEDDVRRFVVRRPLPAKDNKKATSKAPKIQRLITPVV 185          
BLAST of 40S ribosomal protein S6 vs. Ensembl Fly
Match: RpS6 (gene:FBgn0261592 transcript:FBtr0071136)

HSP 1 Score: 209.534 bits (532), Expect = 1.423e-67
Identity = 107/153 (69.93%), Postives = 127/153 (83.01%), Query Frame = 3
Query:  156 ISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPI 614
            +  +V AD LGD WKGY L+I GGNDKQGFPM QGVLT+ RVRLLLKKG  CYR RR GERKRKSVRGCIVD N+SV++  + +KG  +IPGLTDT +P+RLGPKRASKIRKL+NL+ +DDVR++V+RR LP KD KKA SKAPKIQ L+TP+
Sbjct:    1 MGQVVEADILGDEWKGYQLRIAGGNDKQGFPMKQGVLTHGRVRLLLKKGHSCYRPRRTGERKRKSVRGCIVDANMSVLALVVLKKGEKDIPGLTDTTIPRRLGPKRASKIRKLYNLSKEDDVRRFVVRRPLPAKDNKKATSKAPKIQRLITPV 153          
BLAST of 40S ribosomal protein S6 vs. Ensembl Zebrafish
Match: rps6 (ribosomal protein S6 [Source:ZFIN;Acc:ZDB-GENE-040801-8])

HSP 1 Score: 242.276 bits (617), Expect = 6.352e-80
Identity = 126/183 (68.85%), Postives = 152/183 (83.06%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTP 611
            MKLNIS+PA G QK IEVDD  KL+ F +K ++  V+ADSLGD WKGY+++I+GGNDKQGFPM QGVLT+ RVRLLL KG  CYR RR GERKRKSVRGCIVD NLSV++  I +KG  +IPGLTD+ VP+RLGPKRAS+IRKLFNL+ +DDVR+YV+RR L + +GKK ++KAPKIQ LVTP
Sbjct:    1 MKLNISFPATGCQKLIEVDDERKLRIFYEKRMATEVAADSLGDEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVRKGEKDIPGLTDSTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRRPLTK-EGKKPRTKAPKIQRLVTP 182          
BLAST of 40S ribosomal protein S6 vs. Ensembl Xenopus
Match: rps6 (ribosomal protein S6 [Source:NCBI gene;Acc:394757])

HSP 1 Score: 239.965 bits (611), Expect = 7.738e-79
Identity = 137/215 (63.72%), Postives = 169/215 (78.60%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            MKLNIS+PA G QK IEV+D  KL+ F +K ++  V+AD LGD WKGY+++I+GGNDKQGFPM QGVLT+ RVRLLL KG  CYR RR GERKRKSVRGCIVD NLSV++  I +KG  +IPGLTD  VP+RLGPKRAS+IRKLFNL+ +DDVR+YV+R+ L + +GKK ++KAPKIQ LVTP  LQ +RR  ALK+QR  K   +  EY K++A
Sbjct:    1 MKLNISFPATGCQKLIEVEDERKLRTFYEKRMATEVAADPLGDEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVRKGEKDIPGLTDNTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLAK-EGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTQKNKEEASEYAKLLA 214          
BLAST of 40S ribosomal protein S6 vs. Ensembl Xenopus
Match: rps6 (ribosomal protein S6 [Source:NCBI gene;Acc:394757])

HSP 1 Score: 233.417 bits (594), Expect = 2.743e-76
Identity = 134/216 (62.04%), Postives = 168/216 (77.78%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVAL 710
            ++LNIS+PA G QK IEV+D  KL+ F +K ++  V+AD LGD WKGY+++I+GGNDKQGFPM QGVLT+ RVRLLL KG  CYR RR GERKRKSVRGCIVD NLSV++  I +KG  +IPGLTD  VP+RLGPKRAS+IRKLFNL+ +DDVR+YV+R+ L + +GKK ++KAPKIQ LVTP  LQ +RR  ALK+QR  K   +  EY K++  
Sbjct:    6 LQLNISFPATGCQKLIEVEDERKLRTFYEKRMATEVAADPLGDEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVRKGEKDIPGLTDNTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLAK-EGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTQKNKEEASEYAKLLQF 220          
BLAST of 40S ribosomal protein S6 vs. Ensembl Mouse
Match: Rps6 (ribosomal protein S6 [Source:MGI Symbol;Acc:MGI:98159])

HSP 1 Score: 242.276 bits (617), Expect = 6.563e-80
Identity = 139/215 (64.65%), Postives = 171/215 (79.53%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            MKLNIS+PA G QK IEVDD  KL+ F +K ++  V+AD+LG+ WKGY+++I+GGNDKQGFPM QGVLT+ RVRLLL KG  CYR RR GERKRKSVRGCIVD NLSV++  I +KG  +IPGLTDT VP+RLGPKRAS+IRKLFNL+ +DDVR+YV+R+ L  K+GKK ++KAPKIQ LVTP  LQ +RR  ALK+QR  K   +  EY K++A
Sbjct:    1 MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPL-NKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLA 214          
BLAST of 40S ribosomal protein S6 vs. UniProt/SwissProt
Match: sp|Q9BMX5|RS6_APLCA (40S ribosomal protein S6 OS=Aplysia californica OX=6500 GN=RPS6 PE=2 SV=1)

HSP 1 Score: 265.774 bits (678), Expect = 2.624e-88
Identity = 151/215 (70.23%), Postives = 175/215 (81.40%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            MKLNISYPA G QK IEVDD  KL+PF DK IS  +SAD LGD WKGY+++I+GGNDKQGFPM QGVLT  RVRLLL K   CYR RR GERKRKSVRGCIVD+NLSV+S  I +KG  +IPGLTD  +P+RLGPKRASKIRKLFNL+ +DDVR+YV+RR LPE+DGKKAKSKAPKIQ LVTP+ LQR+R   ALK++R +KK  D  EY K++A
Sbjct:    1 MKLNISYPATGCQKLIEVDDEKKLRPFYDKRISAELSADCLGDEWKGYVVRISGGNDKQGFPMKQGVLTATRVRLLLDKNHSCYRPRRTGERKRKSVRGCIVDSNLSVLSLVIIKKGEQDIPGLTDKTIPRRLGPKRASKIRKLFNLSKEDDVRQYVVRRPLPERDGKKAKSKAPKIQRLVTPVVLQRKRARMALKKKRVTKKREDHAEYTKLLA 215          
BLAST of 40S ribosomal protein S6 vs. UniProt/SwissProt
Match: sp|O01727|RS6_BRAFL (40S ribosomal protein S6 OS=Branchiostoma floridae OX=7739 GN=RPS6 PE=3 SV=1)

HSP 1 Score: 256.144 bits (653), Expect = 1.403e-84
Identity = 140/215 (65.12%), Postives = 173/215 (80.47%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            MK+NISYPA G QK IEVDD  KL+PF +K +S  ++A+SLGD WKGYL++I+GGNDKQGFPM QGVLTN RVRLLL KG  CYR RR GERKRKSVRGCIVD+NLSV++  I +KG  +IPGLTDT +P+RLGPKRA +IRKLFNL  +DDVR+YV+RR LP+K+GKK K K PKIQ L+TP  LQR+R + A+KR+R +K+  +   Y K++A
Sbjct:    1 MKINISYPATGCQKLIEVDDERKLRPFYEKRMSHQMTAESLGDEWKGYLVRISGGNDKQGFPMKQGVLTNGRVRLLLGKGHSCYRPRRTGERKRKSVRGCIVDSNLSVLNLVILKKGEQDIPGLTDTTIPRRLGPKRAGRIRKLFNLNKEDDVRQYVVRRPLPQKEGKKQKFKTPKIQRLITPQRLQRKRHMRAVKRRRYAKQREEEATYAKLLA 215          
BLAST of 40S ribosomal protein S6 vs. UniProt/SwissProt
Match: sp|Q2PQM1|RS6_GLOMM (40S ribosomal protein S6 OS=Glossina morsitans morsitans OX=37546 GN=RpS6 PE=2 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 2.915e-84
Identity = 131/185 (70.81%), Postives = 151/185 (81.62%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPIT 617
            MKLN+SYPA G QK  EV+D HKL+ F +KH+  +V AD LGD WKGY+L+I+GGNDKQGFPM QGVLT+ RVRLLLKKG  CYR RR GERKRKSVRGCIVD N+SV++  I +KG  +IPGLTDT VP+RLGPKRASKIRKL+NL+  DDVR YVIRR LP KD KKA SKAPKIQ L+TP+ 
Sbjct:    1 MKLNVSYPATGCQKLFEVNDEHKLRVFYEKHMGQVVEADILGDEWKGYMLRISGGNDKQGFPMKQGVLTHDRVRLLLKKGHSCYRPRRTGERKRKSVRGCIVDANMSVLALVIVKKGEKDIPGLTDTSVPRRLGPKRASKIRKLYNLSKVDDVRPYVIRRPLPAKDNKKAISKAPKIQRLITPVV 185          
BLAST of 40S ribosomal protein S6 vs. UniProt/SwissProt
Match: sp|Q9U761|RS6_AEDAE (40S ribosomal protein S6 OS=Aedes aegypti OX=7159 GN=RpS6 PE=2 SV=2)

HSP 1 Score: 252.292 bits (643), Expect = 1.319e-81
Identity = 139/216 (64.35%), Postives = 170/216 (78.70%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVAL 710
            MKLN+S+PA G+QK  EV D HKL+ F DK +   ++AD LGD WKGY+ KI GGNDKQGFPM QGVLTN RVRLLLKKG  CYR RR GERKRKSVRGCIVD NLS ++  + +KG  +IPGLTDT VP+RLGPKRAS IRKL+NLT +DDVR++V++R LPEKDGKKA++KAPKIQ L+TP+ LQR+R   A+K++R   +     EY+KI+ L
Sbjct:    1 MKLNVSFPATGAQKTFEVMDDHKLRHFYDKRMGAEITADHLGDEWKGYVFKIAGGNDKQGFPMKQGVLTNTRVRLLLKKGHSCYRPRRTGERKRKSVRGCIVDQNLSALALIVVKKGEKDIPGLTDTTVPRRLGPKRASNIRKLYNLTKEDDVRQFVVKRPLPEKDGKKARTKAPKIQRLITPVVLQRKRHRLAIKKRRVESRKEAEAEYMKILHL 216          
BLAST of 40S ribosomal protein S6 vs. UniProt/SwissProt
Match: sp|P29327|RS6_DROME (40S ribosomal protein S6 OS=Drosophila melanogaster OX=7227 GN=RpS6 PE=1 SV=1)

HSP 1 Score: 247.669 bits (631), Expect = 3.759e-81
Identity = 126/185 (68.11%), Postives = 149/185 (80.54%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPIT 617
            MKLN+SYPA G QK  EV D HKL+ F +K +  +V AD LGD WKGY L+I GGNDKQGFPM QGVLT+ RVRLLLKKG  CYR RR GERKRKSVRGCIVD N+SV++  + +KG  +IPGLTDT +P+RLGPKRASKIRKL+NL+ +DDVR++V+RR LP KD KKA SKAPKIQ L+TP+ 
Sbjct:    1 MKLNVSYPATGCQKLFEVVDEHKLRVFYEKRMGQVVEADILGDEWKGYQLRIAGGNDKQGFPMKQGVLTHGRVRLLLKKGHSCYRPRRTGERKRKSVRGCIVDANMSVLALVVLKKGEKDIPGLTDTTIPRRLGPKRASKIRKLYNLSKEDDVRRFVVRRPLPAKDNKKATSKAPKIQRLITPVV 185          
BLAST of 40S ribosomal protein S6 vs. TrEMBL
Match: A0A0B7BUC2 (Uncharacterized protein (Fragment) OS=Arion vulgaris OX=1028688 GN=ORF212646 PE=4 SV=1)

HSP 1 Score: 263.077 bits (671), Expect = 1.330e-84
Identity = 148/216 (68.52%), Postives = 178/216 (82.41%), Query Frame = 3
Query:   60 IMKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            +MKLNIS+PA G QK IEVDD  KL+PF DK +S  VSAD+LGD WKGY+++I+GGNDKQGFPM QGVLT  RVRLLL KG  CYR RR GERKRKSVRGCIVD+ LSV++  I +KG  +IPGLTD+ +P+RLGPKRASKIRKLFNL+ +DDVR+YV+RR LPEKDGKKAKSKAPKIQ LVTP+ LQR+R   ALK++R +KK  +  +Y K++A
Sbjct:    8 VMKLNISFPATGCQKLIEVDDEKKLRPFYDKRMSAEVSADNLGDEWKGYIVRISGGNDKQGFPMKQGVLTTGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDSTLSVLALVIIKKGEQDIPGLTDSTIPRRLGPKRASKIRKLFNLSKEDDVRQYVVRRPLPEKDGKKAKSKAPKIQRLVTPVVLQRKRHRLALKKKRVTKKREENADYTKLLA 223          
BLAST of 40S ribosomal protein S6 vs. TrEMBL
Match: A0A0B6YBR1 (40S ribosomal protein S6 OS=Arion vulgaris OX=1028688 GN=ORF19665 PE=3 SV=1)

HSP 1 Score: 261.536 bits (667), Expect = 4.171e-84
Identity = 136/184 (73.91%), Postives = 156/184 (84.78%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPI 614
            MKLNIS+PA G QK IEVDD  KL+PF DK +S  VSAD+LGD WKGY+++I+GGNDKQGFPM QGVLT  RVRLLL KG  CYR RR GERKRKSVRGCIVD+ LSV++  I +KG  EIPGLTDT +P+RLGPKRASKIRKLFNL+ +DDVR+YV+RR LPEK+GKKAKSKAPKIQ LVTP+
Sbjct:    1 MKLNISFPATGCQKLIEVDDEKKLRPFYDKRMSAEVSADNLGDEWKGYIVRISGGNDKQGFPMKQGVLTTGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDSTLSVLALVIIKKGEQEIPGLTDTTIPRRLGPKRASKIRKLFNLSKEDDVRQYVVRRPLPEKEGKKAKSKAPKIQRLVTPV 184          
BLAST of 40S ribosomal protein S6 vs. TrEMBL
Match: A0A2C9JFP6 (40S ribosomal protein S6 OS=Biomphalaria glabrata OX=6526 GN=106077334 PE=3 SV=1)

HSP 1 Score: 260.766 bits (665), Expect = 1.761e-83
Identity = 144/216 (66.67%), Postives = 174/216 (80.56%), Query Frame = 3
Query:   60 IMKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            + +LNISYPA G QK +EVDD  KL+PF DK I+  V+ADSLGD WKGY+++I+GGNDKQGFPM QGVLT  RVRLLL KG  CYR RR GERKRKSVRGCIVD NLSV++  I +KG  +IPGLTD  +P+RLGPKRASKIRKLFNL  +DDVR+YV+RR LPEK+GKKAKSKAPKIQ LVTP+ LQR+R   A+K++   +K  ++ EY K++A
Sbjct:   17 LFQLNISYPATGCQKLVEVDDERKLRPFYDKRIAAEVAADSLGDEWKGYVVRISGGNDKQGFPMKQGVLTTGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDGNLSVLALVIIKKGEQDIPGLTDKTIPRRLGPKRASKIRKLFNLAKEDDVRQYVVRRPLPEKEGKKAKSKAPKIQRLVTPVVLQRKRFKLAIKKKALLRKREEQAEYTKLLA 232          
BLAST of 40S ribosomal protein S6 vs. TrEMBL
Match: A0A0R3RYT7 (40S ribosomal protein S6 OS=Elaeophora elaphi OX=1147741 PE=3 SV=1)

HSP 1 Score: 258.455 bits (659), Expect = 5.655e-83
Identity = 145/215 (67.44%), Postives = 171/215 (79.53%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            MKLNI+YP  G QK  E+DD  KL+ F +K ++  V AD+LGD WKGY+L+ITGGNDKQGFPM QGVLTN RVRLLL KG  CYR RR GERKRKSVRGCIVD NLS ++  I +KG  EIPGLTDT VP+RLGPKRASKIRKLFNLT  DDVRKYVIRR+LPEK+GKK +SKAPKIQ L+TP+ LQR+RR  A+K +R  K+  +  +Y K++A
Sbjct:    1 MKLNIAYPTTGCQKVFEIDDEKKLRIFYEKRMAQEVEADALGDEWKGYVLRITGGNDKQGFPMKQGVLTNGRVRLLLSKGHSCYRSRRDGERKRKSVRGCIVDANLSALALIIVKKGEQEIPGLTDTTVPRRLGPKRASKIRKLFNLTKNDDVRKYVIRRKLPEKEGKKPRSKAPKIQRLITPVVLQRKRRRLAMKIKRSVKRREEEAQYHKMMA 215          
BLAST of 40S ribosomal protein S6 vs. TrEMBL
Match: A0A2G8KW88 (40S ribosomal protein S6 OS=Stichopus japonicus OX=307972 GN=BSL78_10836 PE=3 SV=1)

HSP 1 Score: 258.84 bits (660), Expect = 7.519e-83
Identity = 139/213 (65.26%), Postives = 172/213 (80.75%), Query Frame = 3
Query:   69 LNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            LNIS+PA G QK IEVDD HKL+ F DK ++  VSA+SLGD WKGY+++I+GGNDKQGFPM QGVL + RVRLLL KG  CYR RR GERKRKSVRGC+VD+NLSV+S  + +KG NEIPGLTD+ +P+RLGPKRA KIRKLFNL+ +DDVR+YV+RR LP K+G K K K+PKIQ LVTP+ LQR+R L A+K++R  K+  D  +Y K++A
Sbjct:   21 LNISFPATGCQKLIEVDDEHKLRAFFDKRMAQEVSAESLGDEWKGYVVRISGGNDKQGFPMKQGVLVHTRVRLLLSKGHSCYRPRRKGERKRKSVRGCVVDSNLSVLSLVVVKKGENEIPGLTDSTIPRRLGPKRAGKIRKLFNLSKEDDVRQYVVRRPLPLKEGHKQKYKSPKIQRLVTPVRLQRKRHLKAMKKRRAEKRREDAADYAKLLA 233          
BLAST of 40S ribosomal protein S6 vs. Ensembl Cavefish
Match: rps6 (ribosomal protein S6 [Source:NCBI gene;Acc:103029232])

HSP 1 Score: 243.817 bits (621), Expect = 1.320e-80
Identity = 140/215 (65.12%), Postives = 169/215 (78.60%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            MKLNIS+PA G QK IEVDD HKL+ F +K ++  V AD LGD WKGY ++I+GGNDKQGFPM QGVLT+ RVRLLL KG  CYR RR GERKRKSVRGCIVD NLSV++  I +KG  +IPGLTD+ VP+RLGPKRAS+IRKLFNL+ +DDVR+YV+RR L + +GKK ++KAPKIQ LVTP  LQ +R+  ALKRQR  K   +  EY K++A
Sbjct:    1 MKLNISFPATGCQKLIEVDDEHKLRSFYEKRMAAEVPADPLGDEWKGYYVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIIKKGEKDIPGLTDSTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRRPLTK-EGKKPRTKAPKIQRLVTPRVLQHKRQRIALKRQRTQKNKEEAAEYAKMLA 214          
BLAST of 40S ribosomal protein S6 vs. Ensembl Sea Lamprey
Match: rps6 (ribosomal protein S6 [Source:ZFIN;Acc:ZDB-GENE-040801-8])

HSP 1 Score: 246.899 bits (629), Expect = 1.897e-82
Identity = 130/183 (71.04%), Postives = 151/183 (82.51%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTP 611
            MKLNIS+PA G QK IEVDD  KL+ F +K ++  VSAD LGD WKGY+++I+GGNDKQGFPM QGVLTN RVRLLL KG  CYR RR GERKRKSVRGCIVD NLSV++  I +KG  +IPGLTDT +P+RLGPKRASKIRKLFNL+ +DDVRKYV+RR L + +GKK +SKAPKIQ LVTP
Sbjct:    1 MKLNISFPATGCQKLIEVDDERKLRSFYEKRMATEVSADCLGDEWKGYVVRISGGNDKQGFPMKQGVLTNGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTIPRRLGPKRASKIRKLFNLSKEDDVRKYVVRRPLTK-EGKKPRSKAPKIQRLVTP 182          
BLAST of 40S ribosomal protein S6 vs. Ensembl Yeast
Match: RPS6A (Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication [Source:SGD;Acc:S000006011])

HSP 1 Score: 210.305 bits (534), Expect = 1.425e-68
Identity = 111/183 (60.66%), Postives = 137/183 (74.86%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTP 611
            MKLNISYP  GSQK  E+DD H+++ F DK I   V  +++GD +KGY+ KI+GGNDKQGFPM QGVL   R++LLL K   CYR RR GERKRKSVRG IV  +L+V++  I +KG  E+ GLTDT VPKRLGPKRA+ IRK F L+ +DDVR +VIRRE+ +  G+K  +KAPKIQ LVTP
Sbjct:    1 MKLNISYPVNGSQKTFEIDDEHRIRVFFDKRIGQEVDGEAVGDEFKGYVFKISGGNDKQGFPMKQGVLLPTRIKLLLTKNVSCYRPRRDGERKRKSVRGAIVGPDLAVLALVIVKKGEQELEGLTDTTVPKRLGPKRANNIRKFFGLSKEDDVRDFVIRREVTK--GEKTYTKAPKIQRLVTP 181          
BLAST of 40S ribosomal protein S6 vs. Ensembl Yeast
Match: RPS6B (Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication [Source:SGD;Acc:S000000385])

HSP 1 Score: 210.305 bits (534), Expect = 1.425e-68
Identity = 111/183 (60.66%), Postives = 137/183 (74.86%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTP 611
            MKLNISYP  GSQK  E+DD H+++ F DK I   V  +++GD +KGY+ KI+GGNDKQGFPM QGVL   R++LLL K   CYR RR GERKRKSVRG IV  +L+V++  I +KG  E+ GLTDT VPKRLGPKRA+ IRK F L+ +DDVR +VIRRE+ +  G+K  +KAPKIQ LVTP
Sbjct:    1 MKLNISYPVNGSQKTFEIDDEHRIRVFFDKRIGQEVDGEAVGDEFKGYVFKISGGNDKQGFPMKQGVLLPTRIKLLLTKNVSCYRPRRDGERKRKSVRGAIVGPDLAVLALVIVKKGEQELEGLTDTTVPKRLGPKRANNIRKFFGLSKEDDVRDFVIRREVTK--GEKTYTKAPKIQRLVTP 181          
BLAST of 40S ribosomal protein S6 vs. Ensembl Nematostella
Match: EDO37221 (40S ribosomal protein S6 [Source:UniProtKB/TrEMBL;Acc:A7SGC3])

HSP 1 Score: 245.358 bits (625), Expect = 1.209e-81
Identity = 140/215 (65.12%), Postives = 169/215 (78.60%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            MKLNISYP  G QK IE++D  K++ F DK ++  VS + LGD WKGY+ +ITGGNDKQGFPM QG++TN RVRLLL KG  CYR RR GERKRKSVRGCIVD+ LSV+S  I +KG  +IPGLTD  +P+RLGPKR  KIRK+FNL+ +DDVR+YVIRR LPEK+GKKAKSKAPKIQ LVTP+ LQR+R+  ALKRQR  K   +  +Y K++A
Sbjct:    1 MKLNISYPVTGCQKLIEIEDERKVRSFYDKRMAMEVSGECLGDEWKGYVFRITGGNDKQGFPMKQGIMTNGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDSQLSVLSLVIVKKGEQDIPGLTDNTIPRRLGPKRVGKIRKMFNLSKEDDVRQYVIRRPLPEKEGKKAKSKAPKIQRLVTPVVLQRKRKRLALKRQRAQKCKQEAADYAKLLA 215          
BLAST of 40S ribosomal protein S6 vs. Ensembl Medaka
Match: rps6 (ribosomal protein S6 [Source:NCBI gene;Acc:101162903])

HSP 1 Score: 243.047 bits (619), Expect = 2.287e-80
Identity = 140/215 (65.12%), Postives = 168/215 (78.14%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            MKLNIS+PA G QK IEVDD  KL+ F +K ++  V+AD LGD WKGY+++I+GGNDKQGFPM QGVLT  RVRLLL KG  CYR RR GERKRKSVRGCIVD NLSV++  I +KG  +IPGLTD+ VP+RLGPKRASKIRKLFNL  +DDVR+YV+RR L + +GKK ++KAPKIQ LVTP  LQ +RR  ALK+QR  K   +  EY K++A
Sbjct:    1 MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADPLGDEWKGYVVRISGGNDKQGFPMKQGVLTQGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIIKKGEKDIPGLTDSTVPRRLGPKRASKIRKLFNLAKEDDVRQYVVRRPLTK-EGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTQKNKEEASEYAKLLA 214          
BLAST of 40S ribosomal protein S6 vs. Ensembl Medaka
Match: rps6 (ribosomal protein S6 [Source:NCBI gene;Acc:101162903])

HSP 1 Score: 240.736 bits (613), Expect = 1.604e-78
Identity = 139/216 (64.35%), Postives = 168/216 (77.78%), Query Frame = 3
Query:   60 IMKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVA 707
            + KLNIS+PA G QK IEVDD  KL+ F +K ++  V+AD LGD WKGY+++I+GGNDKQGFPM QGVLT  RVRLLL KG  CYR RR GERKRKSVRGCIVD NLSV++  I +KG  +IPGLTD+ VP+RLGPKRASKIRKLFNL  +DDVR+YV+RR L + +GKK ++KAPKIQ LVTP  LQ +RR  ALK+QR  K   +  EY K++A
Sbjct:   59 VNKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADPLGDEWKGYVVRISGGNDKQGFPMKQGVLTQGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIIKKGEKDIPGLTDSTVPRRLGPKRASKIRKLFNLAKEDDVRQYVVRRPLTK-EGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTQKNKEEASEYAKLLA 273          
BLAST of 40S ribosomal protein S6 vs. Planmine SMEST
Match: SMESG000000674.1 (SMESG000000674.1)

HSP 1 Score: 377.481 bits (968), Expect = 1.104e-133
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 3
Query:   63 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEXXXXXXXXXXXXIQNLVTPITXXXXXXXXXXXXXXXSKKIADREEYVKIVAL 710
            MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEKDGKKAKSKAPKIQNLVTPITLQRRRRLDALKRQRKSKKIADREEYVKIVAL
Sbjct:    1 MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLLKITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGCIVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPKDDVRKYVIRRELPEKDGKKAKSKAPKIQNLVTPITLQRRRRLDALKRQRKSKKIADREEYVKIVAL 216          
The following BLAST results are available for this feature:
BLAST of 40S ribosomal protein S6 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
RPS69.559e-8064.65ribosomal protein S6 [Source:HGNC Symbol;Acc:HGNC:... [more]
RPS61.351e-6466.11ribosomal protein S6 [Source:HGNC Symbol;Acc:HGNC:... [more]
RPS61.351e-6466.11ribosomal protein S6 [Source:HGNC Symbol;Acc:HGNC:... [more]
RPS63.554e-1158.70ribosomal protein S6 [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of 40S ribosomal protein S6 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
rps-63.752e-7362.7040S ribosomal protein S6 [Source:UniProtKB/Swiss-... [more]
rps-61.179e-4548.6540S ribosomal protein S6 [Source:UniProtKB/Swiss-... [more]
rps-61.222e-4265.0440S ribosomal protein S6 [Source:UniProtKB/Swiss-... [more]
rps-61.222e-4265.0440S ribosomal protein S6 [Source:UniProtKB/Swiss-... [more]
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BLAST of 40S ribosomal protein S6 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
RpS63.750e-8268.11gene:FBgn0261592 transcript:FBtr0071135[more]
RpS61.423e-6769.93gene:FBgn0261592 transcript:FBtr0071136[more]
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BLAST of 40S ribosomal protein S6 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rps66.352e-8068.85ribosomal protein S6 [Source:ZFIN;Acc:ZDB-GENE-040... [more]
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BLAST of 40S ribosomal protein S6 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rps67.738e-7963.72ribosomal protein S6 [Source:NCBI gene;Acc:394757][more]
rps62.743e-7662.04ribosomal protein S6 [Source:NCBI gene;Acc:394757][more]
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BLAST of 40S ribosomal protein S6 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Rps66.563e-8064.65ribosomal protein S6 [Source:MGI Symbol;Acc:MGI:98... [more]
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BLAST of 40S ribosomal protein S6 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9BMX5|RS6_APLCA2.624e-8870.2340S ribosomal protein S6 OS=Aplysia californica OX... [more]
sp|O01727|RS6_BRAFL1.403e-8465.1240S ribosomal protein S6 OS=Branchiostoma floridae... [more]
sp|Q2PQM1|RS6_GLOMM2.915e-8470.8140S ribosomal protein S6 OS=Glossina morsitans mor... [more]
sp|Q9U761|RS6_AEDAE1.319e-8164.3540S ribosomal protein S6 OS=Aedes aegypti OX=7159 ... [more]
sp|P29327|RS6_DROME3.759e-8168.1140S ribosomal protein S6 OS=Drosophila melanogaste... [more]
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BLAST of 40S ribosomal protein S6 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0B7BUC21.330e-8468.52Uncharacterized protein (Fragment) OS=Arion vulgar... [more]
A0A0B6YBR14.171e-8473.9140S ribosomal protein S6 OS=Arion vulgaris OX=1028... [more]
A0A2C9JFP61.761e-8366.6740S ribosomal protein S6 OS=Biomphalaria glabrata ... [more]
A0A0R3RYT75.655e-8367.4440S ribosomal protein S6 OS=Elaeophora elaphi OX=1... [more]
A0A2G8KW887.519e-8365.2640S ribosomal protein S6 OS=Stichopus japonicus OX... [more]
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BLAST of 40S ribosomal protein S6 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rps61.320e-8065.12ribosomal protein S6 [Source:NCBI gene;Acc:1030292... [more]
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BLAST of 40S ribosomal protein S6 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rps61.897e-8271.04ribosomal protein S6 [Source:ZFIN;Acc:ZDB-GENE-040... [more]
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BLAST of 40S ribosomal protein S6 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
RPS6A1.425e-6860.66Protein component of the small (40S) ribosomal sub... [more]
RPS6B1.425e-6860.66Protein component of the small (40S) ribosomal sub... [more]
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BLAST of 40S ribosomal protein S6 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO372211.209e-8165.1240S ribosomal protein S6 [Source:UniProtKB/TrEMBL... [more]
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BLAST of 40S ribosomal protein S6 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rps62.287e-8065.12ribosomal protein S6 [Source:NCBI gene;Acc:1011629... [more]
rps61.604e-7864.35ribosomal protein S6 [Source:NCBI gene;Acc:1011629... [more]
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BLAST of 40S ribosomal protein S6 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000000674.11.104e-133100.00SMESG000000674.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30013018 ID=SMED30013018|Name=40S ribosomal protein S6|organism=Schmidtea mediterranea sexual|type=transcript|length=811bp
ATATAGTAAAAATTGTTTAAATAAATTGATTAAATTTGGTATAAAATTTT
TAAAATTAGATAATGAAGCTTAATATATCTTATCCCGCATTGGGCTCTCA
AAAATGCATAGAAGTTGATGACATCCATAAACTTAAGCCTTTTATGGACA
AACACATCTCAGACATTGTATCTGCTGATTCTCTTGGAGATGCATGGAAA
GGTTATCTTCTAAAAATTACTGGTGGTAACGACAAACAAGGTTTCCCAAT
GATGCAAGGAGTTTTGACCAATCTCCGTGTCAGACTCCTTTTGAAAAAAG
GATCGAAATGCTACAGAGAAAGAAGAGCTGGTGAACGTAAAAGGAAAAGT
GTTCGTGGTTGCATTGTTGACAACAATCTCTCTGTAATCAGTTTTACTAT
TGAACAAAAAGGTACCAATGAAATTCCCGGTTTGACCGATACTGAAGTTC
CAAAGAGACTTGGACCAAAAAGAGCATCTAAGATCCGAAAACTTTTTAAC
TTAACTCCAAAAGATGATGTTCGAAAATACGTTATCAGACGCGAGTTACC
AGAAAAAGATGGCAAAAAAGCCAAAAGCAAGGCACCAAAAATTCAAAATT
TGGTTACTCCAATTACTTTGCAACGAAGAAGACGATTAGACGCTCTTAAG
AGACAAAGAAAAAGCAAGAAAATTGCTGATAGAGAAGAATATGTCAAAAT
TGTTGCTCTCAGAAACCGTGATGAGAGAGATCGTAAGAGATCAAGATCTC
GTTCAATGAGAGAAAGTCAAAATGCTTAATTATTTATCAGGCTTAATAAA
TTATTGGATTT
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protein sequence of SMED30013018-orf-1

>SMED30013018-orf-1 ID=SMED30013018-orf-1|Name=SMED30013018-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=239bp
MKLNISYPALGSQKCIEVDDIHKLKPFMDKHISDIVSADSLGDAWKGYLL
KITGGNDKQGFPMMQGVLTNLRVRLLLKKGSKCYRERRAGERKRKSVRGC
IVDNNLSVISFTIEQKGTNEIPGLTDTEVPKRLGPKRASKIRKLFNLTPK
DDVRKYVIRRELPEKDGKKAKSKAPKIQNLVTPITLQRRRRLDALKRQRK
SKKIADREEYVKIVALRNRDERDRKRSRSRSMRESQNA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000039gamma neoblast
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR001377Ribosomal_S6e
IPR014401Ribosomal_S6_euk
IPR018282Ribosomal_S6e_CS
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
GO:0005840ribosome
GO:0030529intracellular ribonucleoprotein complex
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
Vocabulary: biological process
TermDefinition
GO:0006412translation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001377Ribosomal protein S6eSMARTSM01405Ribosomal_S6e_2coord: 1..126
e-value: 1.3E-71
score: 253.9
IPR001377Ribosomal protein S6ePFAMPF01092Ribosomal_S6ecoord: 1..126
e-value: 1.5E-52
score: 176.8
IPR001377Ribosomal protein S6ePANTHERPTHR1150240S RIBOSOMAL PROTEIN S6coord: 1..235
IPR014401Ribosomal protein S6, eukaryoticPIRSFPIRSF002129RPS6ecoord: 1..238
e-value: 2.8E-108
score: 358.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 216..232
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 216..238
IPR018282Ribosomal protein S6e, conserved sitePROSITEPS00578RIBOSOMAL_S6Ecoord: 52..63