Cytochrome b-c1 complex subunit 6

Overview
NameCytochrome b-c1 complex subunit 6
Smed IDSMED30012993
Length (bp)331
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Cytochrome b-c1 complex subunit 6 (SMED30012993) t-SNE clustered cells

Violin plots show distribution of expression levels for Cytochrome b-c1 complex subunit 6 (SMED30012993) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Cytochrome b-c1 complex subunit 6 (SMED30012993) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Cytochrome b-c1 complex subunit 6 (SMED30012993) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012993

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30012993SMESG000040616.1 SmedASXL_018742SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
gutSMED30012993SMESG000040616.1 dd_Smed_v4_277_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012993SMESG000040616.1 dd_Smed_v4_277_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30012993SMESG000040616.1 dd_Smed_v4_277_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012993SMESG000040616.1 Contig34282newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012993SMESG000040616.1 Contig34282uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30012993SMESG000040616.1 SMESG000061905.1 OX_Smed_1.0.10144ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Human
Match: UQCRH (ubiquinol-cytochrome c reductase hinge protein [Source:HGNC Symbol;Acc:HGNC:12590])

HSP 1 Score: 50.8322 bits (120), Expect = 4.195e-9
Identity = 23/62 (37.10%), Postives = 35/62 (56.45%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P   ++E+C++ + C+K  +R + C +RV       E C EE FD LH  DHC A ++F  L
Sbjct:   29 PLTTVREQCEQLEKCVKARERLELCDERVSSRSHTEEDCTEELFDFLHARDHCVAHKLFNNL 90
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Human
Match: UQCRHL (ubiquinol-cytochrome c reductase hinge protein like [Source:HGNC Symbol;Acc:HGNC:51714])

HSP 1 Score: 45.0542 bits (105), Expect = 7.203e-7
Identity = 21/62 (33.87%), Postives = 33/62 (53.23%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P   ++E+C++ + C+K  +R +   + V       E C EE FD LH  DHC A ++F  L
Sbjct:   29 PLTTVREQCEQLEKCVKARERLELYDEHVSSRSHTEEDCTEELFDFLHAKDHCVAHKLFNNL 90
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Fly
Match: UQCR-11L (gene:FBgn0050354 transcript:FBtr0088702)

HSP 1 Score: 47.7506 bits (112), Expect = 2.756e-8
Identity = 23/62 (37.10%), Postives = 31/62 (50.00%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P   L+EKC+   H      +Y  C  RV    +  E+C EE FD +  +DHC A  +F KL
Sbjct:   24 PQAALREKCQAKGHIASLYNKYQECNDRVNGKSKTTETCMEELFDFVAELDHCVAHSLFSKL 85
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Fly
Match: UQCR-11L (gene:FBgn0050354 transcript:FBtr0333002)

HSP 1 Score: 47.7506 bits (112), Expect = 2.756e-8
Identity = 23/62 (37.10%), Postives = 31/62 (50.00%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P   L+EKC+   H      +Y  C  RV    +  E+C EE FD +  +DHC A  +F KL
Sbjct:   24 PQAALREKCQAKGHIASLYNKYQECNDRVNGKSKTTETCMEELFDFVAELDHCVAHSLFSKL 85
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Fly
Match: UQCR-11 (gene:FBgn0260008 transcript:FBtr0301785)

HSP 1 Score: 44.2838 bits (103), Expect = 6.500e-7
Identity = 22/62 (35.48%), Postives = 31/62 (50.00%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P   L+EKC+   H      +Y  C  RV    +  E+C EE FD +  +DHC +  +F KL
Sbjct:   23 PQAVLREKCQAKGHIESLYNKYQECNDRVNGRSKTTETCIEELFDYVAELDHCVSHSLFTKL 84
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Fly
Match: UQCR-11 (gene:FBgn0260008 transcript:FBtr0301783)

HSP 1 Score: 44.2838 bits (103), Expect = 6.500e-7
Identity = 22/62 (35.48%), Postives = 31/62 (50.00%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P   L+EKC+   H      +Y  C  RV    +  E+C EE FD +  +DHC +  +F KL
Sbjct:   23 PQAVLREKCQAKGHIESLYNKYQECNDRVNGRSKTTETCIEELFDYVAELDHCVSHSLFTKL 84
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Fly
Match: UQCR-11 (gene:FBgn0260008 transcript:FBtr0301784)

HSP 1 Score: 44.2838 bits (103), Expect = 6.500e-7
Identity = 22/62 (35.48%), Postives = 31/62 (50.00%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P   L+EKC+   H      +Y  C  RV    +  E+C EE FD +  +DHC +  +F KL
Sbjct:   23 PQAVLREKCQAKGHIESLYNKYQECNDRVNGRSKTTETCIEELFDYVAELDHCVSHSLFTKL 84
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Zebrafish
Match: uqcrh (ubiquinol-cytochrome c reductase hinge protein [Source:ZFIN;Acc:ZDB-GENE-051030-93])

HSP 1 Score: 55.0694 bits (131), Expect = 6.257e-11
Identity = 22/62 (35.48%), Postives = 37/62 (59.68%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P + +++KC++++HC    +R + C  RV    +  E C EE FD LH  DHC A ++F+ +
Sbjct:   27 PLETVRQKCEETEHCAHARERLESCETRVNSRSQTTEDCTEELFDFLHARDHCVAHKVFQSI 88
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Zebrafish
Match: uqcrh (ubiquinol-cytochrome c reductase hinge protein [Source:ZFIN;Acc:ZDB-GENE-051030-93])

HSP 1 Score: 55.0694 bits (131), Expect = 6.325e-11
Identity = 22/62 (35.48%), Postives = 37/62 (59.68%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P + +++KC++++HC    +R + C  RV    +  E C EE FD LH  DHC A ++F+ +
Sbjct:   27 PLETVRQKCEETEHCAHARERLESCETRVNSRSQTTEDCTEELFDFLHARDHCVAHKVFQSI 88
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Zebrafish
Match: uqcrh (ubiquinol-cytochrome c reductase hinge protein [Source:ZFIN;Acc:ZDB-GENE-051030-93])

HSP 1 Score: 55.0694 bits (131), Expect = 6.405e-11
Identity = 22/62 (35.48%), Postives = 37/62 (59.68%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P + +++KC++++HC    +R + C  RV    +  E C EE FD LH  DHC A ++F+ +
Sbjct:   28 PLETVRQKCEETEHCAHARERLESCETRVNSRSQTTEDCTEELFDFLHARDHCVAHKVFQSI 89
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Xenopus
Match: ticrr (TOPBP1-interacting checkpoint and replication regulator [Source:Xenbase;Acc:XB-GENE-963895])

HSP 1 Score: 45.0542 bits (105), Expect = 7.978e-7
Identity = 23/63 (36.51%), Postives = 34/63 (53.97%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAA-KEIFKKL 236
            P   ++E C++S+ C+K  +  + C  RV       E C EE FD LH  DHC + +EI K +
Sbjct:   29 PLTTVREHCEQSEKCVKAREILELCETRVNSRSHTEEECTEELFDFLHARDHCVSNREICKNI 91
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Mouse
Match: Uqcrh (ubiquinol-cytochrome c reductase hinge protein [Source:MGI Symbol;Acc:MGI:1913826])

HSP 1 Score: 52.373 bits (124), Expect = 7.897e-10
Identity = 24/62 (38.71%), Postives = 35/62 (56.45%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P   ++E C++ + C+K  +R + C  RV    +  E C EE FD LH  DHC A ++FK L
Sbjct:   27 PLTTVREHCEQLEKCVKARERLELCDNRVSSRSQTEEDCTEELFDFLHARDHCVAHKLFKNL 88
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. UniProt/SwissProt
Match: sp|Q5M9I5|QCR6_RAT (Cytochrome b-c1 complex subunit 6, mitochondrial OS=Rattus norvegicus OX=10116 GN=Uqcrh PE=3 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 2.894e-9
Identity = 24/62 (38.71%), Postives = 36/62 (58.06%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P   ++E C++ + C+K  +R + C +RV    +  E C EE FD LH  DHC A ++FK L
Sbjct:   27 PLTTVREHCEQLEKCVKARERLESCDRRVSSRSQTEEDCTEELFDFLHARDHCVAHKLFKSL 88
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. UniProt/SwissProt
Match: sp|P99028|QCR6_MOUSE (Cytochrome b-c1 complex subunit 6, mitochondrial OS=Mus musculus OX=10090 GN=Uqcrh PE=1 SV=2)

HSP 1 Score: 52.373 bits (124), Expect = 5.524e-9
Identity = 24/62 (38.71%), Postives = 35/62 (56.45%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P   ++E C++ + C+K  +R + C  RV    +  E C EE FD LH  DHC A ++FK L
Sbjct:   27 PLTTVREHCEQLEKCVKARERLELCDNRVSSRSQTEEDCTEELFDFLHARDHCVAHKLFKNL 88
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. UniProt/SwissProt
Match: sp|P07919|QCR6_HUMAN (Cytochrome b-c1 complex subunit 6, mitochondrial OS=Homo sapiens OX=9606 GN=UQCRH PE=1 SV=2)

HSP 1 Score: 50.8322 bits (120), Expect = 2.015e-8
Identity = 23/62 (37.10%), Postives = 35/62 (56.45%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P   ++E+C++ + C+K  +R + C +RV       E C EE FD LH  DHC A ++F  L
Sbjct:   29 PLTTVREQCEQLEKCVKARERLELCDERVSSRSHTEEDCTEELFDFLHARDHCVAHKLFNNL 90
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. UniProt/SwissProt
Match: sp|Q8SPH5|QCR6_MACFA (Cytochrome b-c1 complex subunit 6, mitochondrial OS=Macaca fascicularis OX=9541 GN=UQCRH PE=3 SV=1)

HSP 1 Score: 50.447 bits (119), Expect = 2.967e-8
Identity = 23/62 (37.10%), Postives = 34/62 (54.84%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P   ++E+C++ + C+K  +R + C KRV       E C EE  D LH  DHC A ++F  L
Sbjct:   29 PLTTVREQCEQLEKCVKARERLELCDKRVSSRSRTEEDCTEELLDFLHARDHCVAYKLFNNL 90
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. UniProt/SwissProt
Match: sp|P00126|QCR6_BOVIN (Cytochrome b-c1 complex subunit 6, mitochondrial OS=Bos taurus OX=9913 GN=UQCRH PE=1 SV=2)

HSP 1 Score: 50.0618 bits (118), Expect = 4.609e-8
Identity = 22/62 (35.48%), Postives = 35/62 (56.45%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P   ++E+C++ + C+K  +R + C +RV    +  E C EE  D LH  DHC A ++F  L
Sbjct:   29 PLTTVREQCEQLEKCVKARERLELCDERVSSRSQTEEDCTEELLDFLHARDHCVAHKLFNSL 90
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. TrEMBL
Match: A0A267GDQ2 (Cytochrome b-c1 complex subunit 6 OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig011022g1 PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.165e-14
Identity = 32/60 (53.33%), Postives = 43/60 (71.67%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFK 230
            P D ++EKC+ S HC K + ++++C +RV + P   E CEEE FD+LHC DHC AKEIFK
Sbjct:   18 PIDSIREKCRDSSHCAKYLDKFNKCEERVARRPNTTEDCEEELFDLLHCRDHCVAKEIFK 77
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. TrEMBL
Match: A0A267G9K1 (Cytochrome b-c1 complex subunit 6 OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008739g2 PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 3.737e-14
Identity = 31/60 (51.67%), Postives = 42/60 (70.00%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFK 230
            P D ++EKC+ S HC   + ++++C +RV + P   E CEEE FD+LHC DHC AKEIFK
Sbjct:   18 PMDSIREKCRDSSHCANYLDKFNKCEERVARRPNTTEDCEEELFDLLHCRDHCVAKEIFK 77
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. TrEMBL
Match: A0A267FHK4 (Cytochrome b-c1 complex subunit 6 OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008739g1 PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 3.737e-14
Identity = 31/60 (51.67%), Postives = 42/60 (70.00%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFK 230
            P D ++EKC+ S HC   + ++++C +RV + P   E CEEE FD+LHC DHC AKEIFK
Sbjct:   18 PIDSIREKCRDSSHCANYLDKFNKCEERVARRPNTTEDCEEELFDLLHCRDHCVAKEIFK 77
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. TrEMBL
Match: A0A023FMW3 (Putative ubiquinol-cytochrome c reduct (Fragment) OS=Amblyomma cajennense OX=34607 PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.425e-11
Identity = 31/62 (50.00%), Postives = 37/62 (59.68%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P D +KEKC+    C     +   C  RV    +  E+C EE FD LHCVDHCAAKEIFK+L
Sbjct:   31 PLDTIKEKCRAEGKCSAYGDKLQECNDRVNSRTQTLETCTEELFDFLHCVDHCAAKEIFKQL 92
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. TrEMBL
Match: A0A023FYE4 (Putative ubiquinol-cytochrome c reduct (Fragment) OS=Amblyomma parvum OX=251391 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.919e-11
Identity = 31/62 (50.00%), Postives = 37/62 (59.68%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P D +KEKC+  + C     +   C  RV    +  E+C EE FD LHCVDHCAAKEIFK L
Sbjct:   28 PLDTVKEKCRAENKCAAYGDKLQECNDRVNSRSQTLETCTEELFDFLHCVDHCAAKEIFKHL 89
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Cavefish
Match: ENSAMXT00000051949.1 (cytochrome b-c1 complex subunit 6, mitochondrial-like [Source:NCBI gene;Acc:107197010])

HSP 1 Score: 58.5362 bits (140), Expect = 2.843e-12
Identity = 25/62 (40.32%), Postives = 37/62 (59.68%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P + ++EKC++++HC+   +R + C  RV    E  E C EE FD LH  DHC A ++F  L
Sbjct:   39 PLESIREKCEQTEHCVHARQRLEACEARVGSRSETTEDCTEELFDFLHARDHCVAHKVFSSL 100
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Cavefish
Match: uqcrh (cytochrome b-c1 complex subunit 6, mitochondrial [Source:NCBI gene;Acc:103026398])

HSP 1 Score: 53.9138 bits (128), Expect = 1.403e-10
Identity = 22/62 (35.48%), Postives = 37/62 (59.68%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P + +++KC++++HC+   ++ + C  RV    E  E C EE FD LH  DHC A ++F  +
Sbjct:   28 PLETIRQKCEETEHCVHAREKLEACETRVGSRSETTEDCTEELFDFLHARDHCVAHKVFHSV 89
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Nematostella
Match: EDO39977 (Cytochrome b-c1 complex subunit 6 [Source:UniProtKB/TrEMBL;Acc:A7S8G0])

HSP 1 Score: 50.447 bits (119), Expect = 1.852e-9
Identity = 22/62 (35.48%), Postives = 32/62 (51.61%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P D ++E C++   C K       C +RV       E+C +E  D +HC DHC +K +F KL
Sbjct:   42 PMDAIRESCEEKPECSKLKLELSNCEERVNSKSSTTETCAQELLDFMHCRDHCVSKSLFNKL 103
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Medaka
Match: uqcrh (cytochrome b-c1 complex subunit 6, mitochondrial [Source:NCBI gene;Acc:101155183])

HSP 1 Score: 53.9138 bits (128), Expect = 1.383e-10
Identity = 23/62 (37.10%), Postives = 35/62 (56.45%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P + +++KC+ S+HC+    R ++C  RV       E C EE FD LH  DHC A ++F  +
Sbjct:   30 PLEAVRQKCEASEHCVHAQARLEQCETRVGSRSATDEDCTEELFDFLHARDHCVAHKLFHSV 91
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Medaka
Match: ENSORLT00000010742.3 (cytochrome b-c1 complex subunit 6, mitochondrial [Source:NCBI gene;Acc:101166083])

HSP 1 Score: 52.7582 bits (125), Expect = 4.394e-10
Identity = 22/62 (35.48%), Postives = 35/62 (56.45%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P + ++ KC++++HC+   +R + C  RV       E C EE FD LH  DHC A ++F  +
Sbjct:   24 PLEAIRSKCEQTEHCVHAKERLEMCEARVSSRSNTEEDCTEELFDFLHARDHCVAHKLFHNV 85
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Planmine SMEST
Match: SMESG000040616.1 (SMESG000040616.1)

HSP 1 Score: 127.872 bits (320), Expect = 5.025e-40
Identity = 77/77 (100.00%), Postives = 77/77 (100.00%), Query Frame = 3 
Query:    9 MAXXXXXXXXXXXXPFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKLI 239
            MADNDVNNEEDLVDPFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKLI
Sbjct:    1 MADNDVNNEEDLVDPFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSPERGESCEEEFFDMLHCVDHCAAKEIFKKLI 77
[Close]
BLAST of Cytochrome b-c1 complex subunit 6 vs. Planmine SMEST
Match: SMESG000026771.1 (SMESG000026771.1)

HSP 1 Score: 54.6842 bits (130), Expect = 3.423e-11
Identity = 30/64 (46.88%), Postives = 34/64 (53.12%), Query Frame = 3 
Query:   51 PFDELKEKCKKSDHCIKDVKRYDRCAKRVEQSP--ERGESCEEEFFDMLHCVDHCAAKEIFKKL 236
            P D ++EKCK  DHC K      RC  R+        GE C EE FD LHC DHC A  IF +L
Sbjct:    7 PLDMIQEKCK--DHCTKLNDILVRCTDRINGGKVYAPGEDCSEELFDFLHCRDHCVAGSIFSQL 68
The following BLAST results are available for this feature:
BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
UQCRH4.195e-937.10ubiquinol-cytochrome c reductase hinge protein [So... [more]
UQCRHL7.203e-733.87ubiquinol-cytochrome c reductase hinge protein lik... [more]
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BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
UQCR-11L2.756e-837.10gene:FBgn0050354 transcript:FBtr0088702[more]
UQCR-11L2.756e-837.10gene:FBgn0050354 transcript:FBtr0333002[more]
UQCR-116.500e-735.48gene:FBgn0260008 transcript:FBtr0301785[more]
UQCR-116.500e-735.48gene:FBgn0260008 transcript:FBtr0301783[more]
UQCR-116.500e-735.48gene:FBgn0260008 transcript:FBtr0301784[more]
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BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
uqcrh6.257e-1135.48ubiquinol-cytochrome c reductase hinge protein [So... [more]
uqcrh6.325e-1135.48ubiquinol-cytochrome c reductase hinge protein [So... [more]
uqcrh6.405e-1135.48ubiquinol-cytochrome c reductase hinge protein [So... [more]
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BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ticrr7.978e-736.51TOPBP1-interacting checkpoint and replication regu... [more]
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BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Uqcrh7.897e-1038.71ubiquinol-cytochrome c reductase hinge protein [So... [more]
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BLAST of Cytochrome b-c1 complex subunit 6 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5M9I5|QCR6_RAT2.894e-938.71Cytochrome b-c1 complex subunit 6, mitochondrial O... [more]
sp|P99028|QCR6_MOUSE5.524e-938.71Cytochrome b-c1 complex subunit 6, mitochondrial O... [more]
sp|P07919|QCR6_HUMAN2.015e-837.10Cytochrome b-c1 complex subunit 6, mitochondrial O... [more]
sp|Q8SPH5|QCR6_MACFA2.967e-837.10Cytochrome b-c1 complex subunit 6, mitochondrial O... [more]
sp|P00126|QCR6_BOVIN4.609e-835.48Cytochrome b-c1 complex subunit 6, mitochondrial O... [more]
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BLAST of Cytochrome b-c1 complex subunit 6 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267GDQ21.165e-1453.33Cytochrome b-c1 complex subunit 6 OS=Macrostomum l... [more]
A0A267G9K13.737e-1451.67Cytochrome b-c1 complex subunit 6 OS=Macrostomum l... [more]
A0A267FHK43.737e-1451.67Cytochrome b-c1 complex subunit 6 OS=Macrostomum l... [more]
A0A023FMW32.425e-1150.00Putative ubiquinol-cytochrome c reduct (Fragment) ... [more]
A0A023FYE42.919e-1150.00Putative ubiquinol-cytochrome c reduct (Fragment) ... [more]
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BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSAMXT00000051949.12.843e-1240.32cytochrome b-c1 complex subunit 6, mitochondrial-l... [more]
uqcrh1.403e-1035.48cytochrome b-c1 complex subunit 6, mitochondrial [... [more]
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BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO399771.852e-935.48Cytochrome b-c1 complex subunit 6 [Source:UniProt... [more]
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BLAST of Cytochrome b-c1 complex subunit 6 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
uqcrh1.383e-1037.10cytochrome b-c1 complex subunit 6, mitochondrial [... [more]
ENSORLT00000010742.34.394e-1035.48cytochrome b-c1 complex subunit 6, mitochondrial [... [more]
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BLAST of Cytochrome b-c1 complex subunit 6 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000040616.15.025e-40100.00SMESG000040616.1[more]
SMESG000026771.13.423e-1146.88SMESG000026771.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012993 ID=SMED30012993|Name=Cytochrome b-c1 complex subunit 6|organism=Schmidtea mediterranea sexual|type=transcript|length=331bp
CAGTTTTTATGGCTGACAACGATGTTAATAATGAAGAAGATTTAGTCGAT
CCATTTGATGAGCTGAAGGAAAAATGCAAAAAGAGTGACCATTGCATCAA
GGATGTCAAACGGTATGACAGATGCGCAAAACGGGTGGAACAAAGTCCTG
AACGAGGAGAAAGTTGCGAAGAAGAATTCTTTGACATGCTTCATTGCGTT
GATCACTGCGCTGCAAAAGAAATATTTAAGAAATTAATATAGGAATTTTT
TTGATAATATAGATACTAGTTAGCTTTCAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000418head
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR036811Ubol_cytC_Rdtase_hinge_dom_sf
IPR012317Poly(ADP-ribose)pol_cat_dom
IPR023184Ubol_cytC_Rdtase_hinge_dom
IPR003422Cyt_b-c1_6
Vocabulary: molecular function
TermDefinition
GO:0003950NAD+ ADP-ribosyltransferase activity
GO:0008121ubiquinol-cytochrome-c reductase activity
Vocabulary: biological process
TermDefinition
GO:0006122mitochondrial electron transport, ubiquinol to cytochrome c
GO:0055114oxidation-reduction process
GO:1902600hydrogen ion transmembrane transport
Vocabulary: cellular component
TermDefinition
GO:0005739mitochondrion
GO:0005743mitochondrial inner membrane
GO:0016020membrane
GO:0070469respiratory chain
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023184Ubiquinol-cytochrome C reductase hinge domainPFAMPF02320UCR_hingecoord: 16..78
e-value: 8.3E-22
score: 77.2
IPR036811Ubiquinol-cytochrome C reductase hinge domain superfamilyGENE3DG3DSA:1.10.287.20coord: 4..79
e-value: 2.4E-25
score: 90.4
IPR036811Ubiquinol-cytochrome C reductase hinge domain superfamilySUPERFAMILYSSF81531Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)coord: 7..78
IPR003422Cytochrome b-c1 complex, subunit 6PIRSFPIRSF000019Bc1_11Kcoord: 1..79
e-value: 1.5E-20
score: 70.8
IPR003422Cytochrome b-c1 complex, subunit 6PANTHERPTHR15336UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 7.8 KDA PROTEINcoord: 9..78