G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30012990
Length (bp)1193
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30012990) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30012990) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30012990) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30012990) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012990

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30012990h1SMcG0008864 dd_Smed_v4_17171_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012990h1SMcG0008864 dd_Smed_v4_17171_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30012990h1SMcG0008864 dd_Smed_v4_17171_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30012990h1SMcG0008864 dd_Smed_v4_17171_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30012990h1SMcG0008864 dd_Smed_v6_17171_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
enteric muscle cellSMED30012990h1SMcG0008864 dd_Smed_v4_17171_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A4S2LRN3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005307 PE=4 SV=1)

HSP 1 Score: 218.009 bits (554), Expect = 3.604e-64
Identity = 135/310 (43.55%), Postives = 180/310 (58.06%), Query Frame = 1 
Query:   46 LNSIYPPIFLLIGVSGNFLSMVIWIKRIRTSPGSTCLFLALMCGTDTYVLVHGCMRHWLKSLMV-GFDIRTILDCKIPLMIFTLFSDLSVWLIVLLCIERSVCVFVPVDAKRICRLRNGTLAFMFTVCCLIAINFHFILTVKVSATRDYCVATSAVAAKFHRAWPYIDFGLYSLVPXXXXXXXXXXXXXXXHXXXXXXXXXXXXX------------------------XXXIFWTVLLMAIAHFLFTMPIGIYFLIPDEHCADAE----VACEIISNMFTMLQQTNHLMHFFLYSITSTLFISDLKRMF 888
            L S+ PPI LLIG+ GN +S+VIW +RI T+ GST LFL+ M  TD YVL+HGC R W   L    FD+RT L CK PL +FT  SDLS W+IVLLCIER +C + P+  ++ICRLRNG++A   T+  L  +N HF  TV V A  D C    A   +F   W Y+DF +YS VPL  + ILNS I++++      + R+  S                           RI  TV  MAI HF  TMPI ++FL  D+ C          C+++  +   LQ TNH++HFF+YS+TS++F++DLKRM 
Sbjct:   16 LYSVVPPILLLIGIPGNLISLVIWSRRITTTLGSTSLFLSAMSLTDAYVLLHGCTRQWSIGLSNRQFDLRTFLGCKCPLFLFTFSSDLSTWIIVLLCIERVICTWQPIKFRQICRLRNGSVALALTIIVLAGVNLHFFWTVLVIA--DSC-DPKAEYNEFANYWHYLDFAIYSFVPLALMLILNSTIILRIQMTQKSMKRTTASVDNSVIIRSQNDGKPSANHSRATARSMRILRTVSCMAICHFTLTMPIVLHFLFQDKFCLTTSGEFLSQCDLVEVLVVSLQMTNHMVHFFIYSLTSSVFVADLKRML 322
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A419PFT4 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Clonorchis sinensis OX=79923 GN=CSKR_651s PE=4 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 6.050e-63
Identity = 133/309 (43.04%), Postives = 179/309 (57.93%), Query Frame = 1 
Query:   46 LNSIYPPIFLLIGVSGNFLSMVIWIKRIRTSPGSTCLFLALMCGTDTYVLVHGCMRHWLKSLMV-GFDIRTILDCKIPLMIFTLFSDLSVWLIVLLCIERSVCVFVPVDAKRICRLRNGTLAFMFTVCCLIAINFHFILTVKVSATRDYCVATSAVAAKFHRAWPYIDFGLYSLVPXXXXXXXXXXXXXXXHXXXXXXXXX------------------------XXXXXXXIFWTVLLMAIAHFLFTMPIGIYFLIPDEHC----ADAEVACEIISNMFTMLQQTNHLMHFFLYSITSTLFISDLKRM 885
            L S+ PPI LLIG+ GN +S VIW +RI ++ GST LFL+ M  TD YVL+HGC R W   L    FD+RT L CK PL +FT  SDLS W+IVLLCIER +C + P+  ++ICRLRNG++A   T+  L  +N HF  TV V A  D C    A   +F   W Y+DF +YS VPL  + ILNS I++++      + R+                          +   RI  TV  MAI HF  TMPI ++FL  D+ C     D    C+++  +   LQ TNH++HFF+YS TS++F++DL+RM
Sbjct:   16 LYSVVPPILLLIGIPGNLISFVIWSRRITSTLGSTSLFLSAMSLTDAYVLLHGCSRQWSIGLSNRQFDLRTFLGCKCPLFLFTFSSDLSTWIIVLLCIERVICTWQPIKFRQICRLRNGSVALALTIIVLAGVNLHFFWTVLVIA--DSC-DPKAEYNEFASYWHYLDFAIYSFVPLALMLILNSTIILRIQMTQKSMKRTGASTDNSVTIRSQHDMKPSANHSRATARSMRILRTVSCMAICHFTLTMPIVLHFLFQDKFCLTTSGDFLSQCDLVEVLVVSLQMTNHMVHFFIYSFTSSVFVADLRRM 321
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A5J4NID3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Paragonimus westermani OX=34504 GN=DEA37_0005246 PE=4 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 4.409e-62
Identity = 120/315 (38.10%), Postives = 178/315 (56.51%), Query Frame = 1 
Query:   52 SIYPPIFLLIGVSGNFLSMVIWIKRIRTSPGSTCLFLALMCGTDTYVLVHGCMRHWLKSLMVG-FDIRTILDCKIPLMIFTLFSDLSVWLIVLLCIERSVCVFVPVDAKRICRLRNGTLAFMFTVCCLIAINFHFILTVKVSATRDYCVATSAVAAKFHRAWPYIDFGLYSLVPXXX-----------------------XXXXXXXXXXXXHXXXXXXXXXXXXXXXXIFWTVLLMAIAHFLFTMPIGIYFLIPDEHCADAE----VACEIISNMFTMLQQTNHLMHFFLYSITSTLFISDLKRMFP---WLIK 903
            SI+P IFL++G+ GN +S++IW++RI ++PGST LFL+ M  T+TYV++HGC RHWL  L     D+R +L C+ P+ +FT+ SDLS WLI+LLC+ER++C + P+  K+ICRL+ G +A +  +  L A N HF  TV+   +R   V    +   FH  W Y DF +YS +PL+                             ++ +   +R   + +  +     RI  TV  MA  HF  TMP+ I+FL  D  C DA       C ++  +   LQ  NH+ HFF+YS TS++F+SDLK++ P   WL +
Sbjct:   24 SIFPVIFLVVGIPGNLISLIIWLRRISSTPGSTSLFLSAMSVTNTYVILHGCARHWLLGLTNERIDLRVLLGCRGPVFVFTVISDLSSWLIILLCVERAICTWKPIQFKQICRLKYGGIALILVIFSLTAFNMHFFWTVETVESRCETVKRFRI---FHIYWTYADFAVYSFLPLIVILVVNSSIILRLRHTQKCVKRDNSSKTTTISMPSENREPLQRTTRKTDGSARILRTVFCMATCHFALTMPVVIFFLCEDTFCVDASGQFIPQCNVLEKLTISLQIANHVTHFFIYSFTSSIFLSDLKKLIPCVAWLFR 335
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A074Z9D1 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_15440 PE=4 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 1.195e-59
Identity = 132/309 (42.72%), Postives = 178/309 (57.61%), Query Frame = 1 
Query:   46 LNSIYPPIFLLIGVSGNFLSMVIWIKRIRTSPGSTCLFLALMCGTDTYVLVHGCMRHWLKSLMV-GFDIRTILDCKIPLMIFTLFSDLSVWLIVLLCIERSVCVFVPVDAKRICRLRNGTLAFMFTVCCLIAINFHFILTVKVSATRDYCVATSAVAAKFHRAWPYIDFGLYSLVPXXXXXXXXXXXXXXXHXXXXXXXXXXXXX------------------------XXXIFWTVLLMAIAHFLFTMPIGIYFLIPDEHCADAE----VACEIISNMFTMLQQTNHLMHFFLYSITSTLFISDLKRM 885
            L S+ PPI LLIG+ GN +S VIW +RI ++ GST LFL+ M  TD YVL+HGC R W   L    FD+RT L CK PL +FT  SDLS W+IVLLCIER +C + P+  ++ICRLRNG++A   T+  L  +N HF  TV V    D C    A   +F   W Y+DF +YS VPL  + ILNS I++++      + R+  S                           RI  TV  MAI HF  TMPI ++FL  D+ C  +       C+++  +   LQ TNH++HFF+YS TS++F++DL+RM
Sbjct:  211 LYSVVPPILLLIGIPGNLISFVIWSRRITSTLGSTSLFLSAMSLTDAYVLLHGCSRQWSIGLSNRQFDLRTFLGCKCPLFLFTFSSDLSTWIIVLLCIERVICTWEPIKFRQICRLRNGSVALALTIIVLAGVNLHFFWTVLV--IEDSC-DPKAEYNEFANYWHYLDFAIYSFVPLALMLILNSTIILRIQMTQKSMKRTSASVDNSVTIRSQHDGKPSTNHSRATARSMRILRTVSCMAICHFTLTMPIVLHFLFQDKFCLTSSGEFLSQCDLVEVLVVSLQMTNHMVHFFIYSFTSSVFVADLRRM 516

HSP 2 Score: 157.918 bits (398), Expect = 1.587e-39
Identity = 88/177 (49.72%), Postives = 110/177 (62.15%), Query Frame = 1 
Query:   46 LNSIYPPIFLLIGVSGNFLSMVIWIKRIRTSPGSTCLFLALMCGTDTYVLVHGCMRHWLKSLMV-GFDIRTILDCKIPLMIFTLFSDLSVWLIVLLCIERSVCVFVPVDAKRICRLRNGTLAFMFTVCCLIAINFHFILTVKVSATRDYCVATSAVAAKFHRAWPY--IDFGLYSLV 567
            L S+ PPI LLIG+ GN +S VIW +RI ++ GST LFL+ M  TD YVL+HGC R W   L    FD+RT L CK PL +FT  SDLS W+IVLLCIER +C + P+  ++ICRLRNG++A   T+  L  +N HF  TV V    D C    A   +F   W Y  I FG+ S V
Sbjct:   16 LYSVVPPILLLIGIPGNLISFVIWSRRITSTLGSTSLFLSAMSLTDAYVLLHGCSRQWSIGLSNRQFDLRTFLGCKCPLFLFTFSSDLSTWIIVLLCIERVICTWEPIKFRQICRLRNGSVALALTIIVLAGVNLHFFWTVLV--IEDSC-DPKAEYNEFANYWHYLCIPFGMCSSV 189
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183A5F6 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS2191 PE=4 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 2.144e-59
Identity = 128/326 (39.26%), Postives = 179/326 (54.91%), Query Frame = 1 
Query:   19 NETLWHLKILN---SIY---PPIFLLIGVSGNFLSMVIWIKRIRTSPGSTCLFLALMCGTDTYVLVHGCMRHWLKSLMVGF-DIRTILDCKIPLMIFTLFSDLSVWLIVLLCIERSVCVFVPVDAKRICRLRNGTLAFMFTVCCLIAINFHFILTVKVSATRDYCVATSAVAAKFHRAWPYIDFGLYSL-----------------------VPXXXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXIFWTVLLMAIAHFLFTMPIGIYFLIPDEHCADAE-----VACEIISNMFTMLQQTNHLMHFFLYSITSTLFISDLKRMFP 891
            NET  H +  N    +Y   PPIF+++G+ GN +SM IW +R+  +PGST LFL  MC TDTYVL HGC+R WL  L     D+R  L C+IPL++FT  +DLS W+I+LLC+ER +    P+  K IC LRNG +A +  V CL+ IN HF+ TV+     + CV  +A +  F  +W +ID  +YS                        V         ++ L  +        R+R+    R+  TVL M I HF FTMPI IY+L     C   +     + C+I+  + T+LQ  NH+ HFF+YS TST+F+SDLK++ P
Sbjct:    8 NETETHQQKSNISLGLYASIPPIFMIVGIPGNLVSMYIWSRRMPMTPGSTSLFLVAMCITDTYVLTHGCLRLWLLGLSKQLIDVRIRLGCRIPLLLFTFTTDLSSWIIILLCVERVISTRFPIRFKSICHLRNGLIALIGLVVCLMGINAHFLWTVQ--RKNNECVPVNAFS-NFMYSWQFIDASIYSFIPLACILALNTCIIIRLRRTAKCVRTGNRVDKGTVKLHSVDIRGGFGVRAREQRSARLLRTVLCMVICHFTFTMPIVIYYLFDAHFCESQDEKSVYLHCDIVETVTTLLQLANHVTHFFIYSFTSTVFLSDLKKLIP 330
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000032545.1 (SMESG000032545.1)

HSP 1 Score: 382.874 bits (982), Expect = 6.786e-134
Identity = 221/222 (99.55%), Postives = 221/222 (99.55%), Query Frame = 1 
Query:  412 MFTVCCLIAINFHFILTVKVSATRDYCVATSAVAAKFHRAWPYIDFGLYSLVPXXXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXIFWTVLLMAIAHFLFTMPIGIYFLIPDEHCADAEVACEIISNMFTMLQQTNHLMHFFLYSITSTLFISDLKRMFPWLIKLKFFRQRVDTSRSRSKAVMDEFECRIKADNQVEKSELITMSHYPIASSVEINMQLTSL 1077
            MFTVCCLIAINFHFILTVKVSATRDYCVATSAVAAKFHRAWPYIDFGLYSLVPLLFIFILNSLILIKLHRAHSRLSRSRQSSIGRIFWTVLLMAIAHFLFTMPIGIYFLIPDEHCADAEVACEIISNMFTMLQQTNHLMHFFLYSITSTLFISDLKRMFPWLIKLKFFRQRVDTSRSRSKAVMDEFECR KADNQVEKSELITMSHYPIASSVEINMQLTSL
Sbjct:    1 MFTVCCLIAINFHFILTVKVSATRDYCVATSAVAAKFHRAWPYIDFGLYSLVPLLFIFILNSLILIKLHRAHSRLSRSRQSSIGRIFWTVLLMAIAHFLFTMPIGIYFLIPDEHCADAEVACEIISNMFTMLQQTNHLMHFFLYSITSTLFISDLKRMFPWLIKLKFFRQRVDTSRSRSKAVMDEFECRTKADNQVEKSELITMSHYPIASSVEINMQLTSL 222
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000061235.1 (SMESG000061235.1)

HSP 1 Score: 80.1073 bits (196), Expect = 3.322e-16
Identity = 54/183 (29.51%), Postives = 93/183 (50.82%), Query Frame = 1 
Query:   52 SIYPPIFLLIGVSGNFLSMVIWIKRIRTSPGSTCLFLALMCGTDTYVLVHGCMRHWLKSLMVG-FDIRTILDCKIPLMIFTLFSDLSVWLIVLLCIERSVCVFVPVDAKRICRLRNGTLAFMFTVCCLIAINFHFILTVKVSATRDYCVAT----SAVAAK-----FHRAWPYIDFGLYSLVP 570
            ++ PPI LLIG  GN L+ ++    +     S+ ++LA+M   D  +LV G +R ++  + +   +  ++L C++   I    S +SVWLIV++ IER + +++P+ AK+I + +        T+ C   +  HF  TV    T+ +            AK     F+R W  +D  LYSL+P
Sbjct:   72 TVIPPIILLIGSIGNVLACIVLCCYL-IDKCSSYIYLAVMVIVDEMILVTGLLRRYIGYIFIKHIENESVLFCRLTQFIGVSSSLVSVWLIVVMTIERVIIIYLPLRAKQITQKKIARAVISSTIICSSLVASHFFFTVDTHVTKQFNKTNLIHFGECTAKPQFHYFYRVWTILDASLYSLIP 253
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000075029.1 (SMESG000075029.1)

HSP 1 Score: 65.855 bits (159), Expect = 1.546e-11
Identity = 53/192 (27.60%), Postives = 93/192 (48.44%), Query Frame = 1 
Query:   40 KILNSIYPPIFLLIGVSGNFLSMVIWIKRIRTSPGSTCLFLALMCGTDTYVLVHGCMRHWLKSLM-VGFDIRTILDCKIPLMIFTLFSDLSVWLIVLLCIERSVCVFVPVDAKRI-CRLRNGTLAFMFTVCCLIAINFHFILTVKVSATRDYCVATSAVAAK-------------FHRAWPYIDFGLYSLVP 570
            K L  I P I L++G  GN  S +I  KR      S+ ++L+ +   D  VL+ G MRH +  ++    +      C +  +I +  S LS WLIV +  ER++ +  P  + R+  R R   +  + ++ CL+A + HF+L+V++   + Y    +  + +             F +AW ++D  LYS +P
Sbjct:   79 KYLWKIVPLILLILGTIGNCTSFIILTKR----RVSSYVYLSALAMADQGVLITGLMRHCIDRILDYRLEDSHHWTCVLTQLIGSSLSYLSAWLIVAVTAERAIVIMFPFSSIRLYSRFRETLVVLLISLACLLA-SSHFLLSVRLETGQMYRFNLNNGSVQNYSQCQLRNELHSFSKAWYWLDGLLYSYLP 265
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000020718.1 (SMESG000020718.1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.380e-10
Identity = 47/182 (25.82%), Postives = 82/182 (45.05%), Query Frame = 1 
Query:   52 SIYPPIFLLIGVSGNFLSMVIWIKRIRTSPGSTCLFLALMCGTDTYVLVHGCMRHWLKSLMV-GFDIRTILDCKIPLMIFTLFSDLSVWLIVLLCIERSVCVFVPVDAKRICRLRNGTLAFMFTVCCLIAINFHFILTVKV--------SATRDYCVATSAVAAKFHRAWPYIDFGLYSLVP 570
            +I P I L  G  GN +S ++ +        ST ++L+L+   D +VL+ G ++ W+  L+    + R    C +     T  S LSVWLIV + +ER++ +  P  A  + +     +  +     ++ +N HFI T+ +        S   ++    S     F   W  ID  +YS +P
Sbjct:   85 TIIPAIILFFGTIGNVMSFIV-LCNFAGKTVSTFIYLSLLAIVDQFVLIFGLLKRWVDYLLKRKIENRNKTFCILSQYFGTSASVLSVWLIVAVTLERAIVISFPFKAHNLIQKSRAIVISISMASVIMILNCHFIFTLDLTNSTQLFESTDNNFVCGFSESYRNFKTVWLLIDAIIYSFLP 265
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000042142.1 (SMESG000042142.1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.364e-9
Identity = 54/191 (28.27%), Postives = 88/191 (46.07%), Query Frame = 1 
Query:   64 PIFLL-IGVSGNFLSMVIWIKRIRTSPGSTCLFLALMCGTDTYVLVHGCMRHWLKSLMV-GFDIRTILDCKIPLMIFTLFSDLSVWLIVLLCIERSVCVFVPVDAKRICRLRNGTLAFMFTVCCLIAINF----HFILTVKVSATRDYCVATSAVA----------------AKFHRAWPYIDFGLYSLVP 570
            PIF++ +G  GNFLS V+  ++   S  S+ ++LA +   D  +L+ G  R W++ +     +  +I  CK+  +I    S +SVWLIV L +ER + +  P +A +  +         + +  LI I+F    HF  TV +       V T+  +                 KF  AW +ID   YS +P
Sbjct:   75 PIFIVAMGSIGNFLSAVVLCRK-SMSHISSYIYLATLAIFDELILLCGLFRKWIEHVTDWSLENHSISFCKLFQVIGVSSSFISVWLIVTLTVERVLIIKRPFEASKYAKASRAK----YIIVSLIIISFTTSCHFFETVTLQNLNHKNVNTNFSSDKGSNSIKKCQFKTEYTKFTNAWFFIDGAFYSWIP 260
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2LRN33.604e-6443.55G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A419PFT46.050e-6343.04G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A5J4NID34.409e-6238.10G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A074Z9D11.195e-5942.72Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A183A5F62.144e-5939.26G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000032545.16.786e-13499.55SMESG000032545.1[more]
SMESG000061235.13.322e-1629.51SMESG000061235.1[more]
SMESG000075029.11.546e-1127.60SMESG000075029.1[more]
SMESG000020718.11.380e-1025.82SMESG000020718.1[more]
SMESG000042142.12.364e-928.27SMESG000042142.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012990 ID=SMED30012990|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1193bp
GAAAAGGGGAAATAAATGAATGAAACTTTATGGCATCTTAAAATTTTAAA
TAGCATTTATCCTCCGATCTTTCTTCTTATCGGAGTTTCAGGAAACTTTC
TGTCAATGGTGATCTGGATCAAACGTATCCGAACGAGTCCCGGATCGACC
TGTCTGTTTCTAGCTCTGATGTGCGGCACTGACACTTACGTGCTCGTCCA
CGGATGCATGAGGCATTGGTTGAAGAGTCTCATGGTAGGTTTCGATATCA
GAACGATATTAGACTGTAAAATCCCATTAATGATTTTTACTCTTTTCAGC
GATTTATCCGTCTGGTTAATCGTTCTGCTTTGTATAGAACGCAGCGTTTG
CGTTTTTGTGCCCGTCGATGCCAAACGGATTTGCCGGTTGCGAAATGGAA
CTCTAGCGTTTATGTTCACTGTCTGCTGTTTGATTGCGATAAATTTCCAC
TTCATCCTCACAGTGAAGGTGTCGGCCACCCGTGATTATTGTGTCGCTAC
GAGTGCCGTTGCCGCCAAATTCCACAGAGCCTGGCCGTACATAGATTTCG
GTTTATATTCTCTTGTGCCGTTGCTTTTTATTTTTATACTCAACTCGCTA
ATACTCATCAAACTGCACCGAGCACACTCGAGATTGAGCAGGTCAAGACA
GTCGAGCATCGGAAGGATATTCTGGACCGTTCTCTTAATGGCTATTGCAC
ATTTTCTCTTTACAATGCCTATTGGGATATATTTCCTCATACCGGATGAG
CATTGCGCAGACGCAGAGGTGGCCTGTGAGATCATATCGAATATGTTCAC
CATGTTACAACAAACGAATCATTTAATGCATTTCTTTTTGTACTCAATCA
CCTCTACCTTGTTCATATCAGACCTGAAACGAATGTTTCCGTGGCTTATA
AAATTGAAATTTTTTCGACAAAGAGTTGATACGTCGAGGTCTCGGTCAAA
AGCTGTGATGGATGAGTTTGAGTGCCGGATCAAAGCTGACAATCAAGTAG
AGAAATCCGAACTGATCACGATGTCCCATTACCCAATTGCCAGTTCCGTA
GAGATCAACATGCAACTCACAAGCTTGTAAAGACGGCCCTTGTCAGGGAA
ACTGATTTGTATATTTGTTGTGATAAAGACAAAATGTCCGAACAAGTCCG
GAAAAGTAAATAAAATGAATATTTTTATGCCATTGAATTGATC
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protein sequence of SMED30012990-orf-1

>SMED30012990-orf-1 ID=SMED30012990-orf-1|Name=SMED30012990-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=106bp
MVNIFDMISQATSASAQCSSGMRKYIPIGIVKRKCAIAIKRTVQNILPML
DCLDLLNLECARCSLMSISELSIKIKSNGTREYKPKSMYGQALWNLAATA
LVATQ*
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protein sequence of SMED30012990-orf-2

>SMED30012990-orf-2 ID=SMED30012990-orf-2|Name=SMED30012990-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=355bp
MNETLWHLKILNSIYPPIFLLIGVSGNFLSMVIWIKRIRTSPGSTCLFLA
LMCGTDTYVLVHGCMRHWLKSLMVGFDIRTILDCKIPLMIFTLFSDLSVW
LIVLLCIERSVCVFVPVDAKRICRLRNGTLAFMFTVCCLIAINFHFILTV
KVSATRDYCVATSAVAAKFHRAWPYIDFGLYSLVPLLFIFILNSLILIKL
HRAHSRLSRSRQSSIGRIFWTVLLMAIAHFLFTMPIGIYFLIPDEHCADA
EVACEIISNMFTMLQQTNHLMHFFLYSITSTLFISDLKRMFPWLIKLKFF
RQRVDTSRSRSKAVMDEFECRIKADNQVEKSELITMSHYPIASSVEINMQ
LTSL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0003104enteric muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR0194277TM_GPCR_serpentine_rcpt_Srw
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: molecular function
TermDefinition
GO:0008528G-protein coupled peptide receptor activity
GO:0004930G-protein coupled receptor activity
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 1..298
e-value: 4.9E-27
score: 96.7
NoneNo IPR availablePANTHERPTHR46641:SF12CNMAMIDE RECEPTORcoord: 9..334
NoneNo IPR availablePANTHERPTHR46641FAMILY NOT NAMEDcoord: 9..334
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 10..283
e-value: 4.41848E-15
score: 72.6656
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 5..299
NoneNo IPR availableTMHMMTMhelixcoord: 218..240
NoneNo IPR availableTMHMMTMhelixcoord: 13..35
NoneNo IPR availableTMHMMTMhelixcoord: 89..107
NoneNo IPR availableTMHMMTMhelixcoord: 175..197
NoneNo IPR availableTMHMMTMhelixcoord: 270..287
NoneNo IPR availableTMHMMTMhelixcoord: 47..69
NoneNo IPR availableTMHMMTMhelixcoord: 128..150
IPR000276G protein-coupled receptor, rhodopsin-likePROSITEPS00237G_PROTEIN_RECEP_F1_1coord: 97..113
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 26..276
score: 15.758