Rapunzel

Overview
NameRapunzel
Smed IDSMED30012932
Length (bp)1366
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012932

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30012932h1SMcG0002320 SmedASXL_007120SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30012932h1SMcG0002320 SmedASXL_007120SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30012932h1SMcG0002320 dd_Smed_v4_811_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012932h1SMcG0002320 dd_Smed_v4_811_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30012932h1SMcG0002320 dd_Smed_v4_811_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30012932h1SMcG0002320 dd_Smed_v4_811_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30012932h1SMcG0002320 dd_Smed_v6_811_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
body wall musculatureSMED30012932h1SMcG0002320 dd_Smed_v4_811_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Rapunzel vs. Ensembl Zebrafish
Match: rpz6 (rapunzel 6 [Source:ZFIN;Acc:ZDB-GENE-170530-5])

HSP 1 Score: 55.4546 bits (132), Expect = 6.262e-8
Identity = 62/255 (24.31%), Postives = 112/255 (43.92%), Query Frame = 2 
Query:  101 QAVLASSKEVFSNLNEVIQAINPGKLFAKLSTVLGALGPAAMAISAVLSIVSMFLPKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALED---AQRENTKEGYQFFAKQYENS-FEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEA---KAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRE 844
            + V+A  KE    + EV++    G     L++ +G L P   A + VL    +FL   +S E+  +K  F  V +KLD+++S+I ++   I+     + +     NI N ++   D   A+ E  +   + F + +  +  E     +YDA+ G++  FG  ++ + +     N   L  FC     L+  G+    A +GY   +   EA   K + W  KI+ +  +    IE C  S+ +      K   +E
Sbjct:    6 ERVVAQKKEA---IEEVMEMFERGA--EVLASAVGGLSPLFEASAPVLR---LFLDNVESKEVTYVKDQFLVVRSKLDVLSSQIEDINCEIKKGRLDSQFFTVEENICNQFRKYIDILEAKPEYKEVRKRLFLQHFPKTGGEKNLYMLYDAVMGNST-FGEPILEVVEQYEARNRRVLEDFCVRLKELLCLGII---ALLGYCFLTQGEEAEQEKIQEWSTKIEEIETKMKETIEKCVQSFPEQAELDIKRLLKE 248
[Close]
BLAST of Rapunzel vs. Ensembl Zebrafish
Match: rpz3 (rapunzel 3 [Source:ZFIN;Acc:ZDB-GENE-030131-4784])

HSP 1 Score: 50.8322 bits (120), Expect = 1.831e-6
Identity = 59/252 (23.41%), Postives = 106/252 (42.06%), Query Frame = 2 
Query:  128 VFSNLNEVIQAINPGKLFAK----LSTVLGALGPAAMAISAVLSIVSMFLPKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALEDAQRENTKEGYQFFAKQYENSFEFATEN------IYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEA---KAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRE 844
            V +   E I+A+   ++F K    L++ +G L P   A + VL +V   L   +S E+  +K  F  V +KLD+++ ++ ++++ I        +     N+ N ++   D      K  Y+   K+      F T        +YDA+ G++  FG  ++ + +     N   L  FC     L+  G+    A +GY   +   EA   K + W  KI  +  +    IE C  S+ +      K   +E
Sbjct:    8 VVAQKKEAIEAVM--EMFEKGAEVLASAVGGLSPLFEAAAPVLKLV---LNNVESKEVTYVKDQFLVVRSKLDVLSCQLQDIDSEIRRRRLDTQFFSVEENMRNQFRKYIDIL--EAKPEYKEVKKRLFLDHFFITGGEKNLCLLYDAVMGNST-FGEPILEVVEQYEARNRRVLEDFCVRLKELLCLGII---ALLGYCFLTQGEEAEQEKIQEWSTKIQEIETKMKETIEKCVQSFPEQAELDIKRLLKE 248
[Close]
BLAST of Rapunzel vs. Ensembl Zebrafish
Match: rpz4 (rapunzel 4 [Source:ZFIN;Acc:ZDB-GENE-050417-472])

HSP 1 Score: 50.0618 bits (118), Expect = 3.262e-6
Identity = 65/294 (22.11%), Postives = 120/294 (40.82%), Query Frame = 2 
Query:  101 QAVLASSKEVFSNLNEVIQAINPGKLFAKLSTVLGALGPAAMAISAVLSIVSMFLPKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALEDAQRENTKEGYQFFAKQ-YENSFEFA-----TENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVR-LSQFCTMGISLINKGLTIEGAYIGY--LNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRELGG-DNQKFAEKIAQDLNNVVPQRSWLAVVYNDASGF 952
            Q ++A  K+V  N+ EV +     ++ A   ++ G L P     S    IV + L   +S E + +K  F+KV ++L++++ ++  +   I+ S   A Y     N+ N ++   D    N K  ++   K+ + + F           +Y A+TG    F    V     N +    R + ++C     L   GL    A +G+  L    + E   + W  K+  +  +   +IE C NS+        K   R+     NQ+ A+ + + L        W   ++N  +G 
Sbjct:    6 QKLVAQKKDVVENVMEVFE--QGAEVVA---SIAGDLFPV---FSIAAPIVRLALDNVESKEAEYMKEQFQKVRDRLEVVSEEVQQINQEIKKSGVDATYFTVEENLTNQFRKFMDVL--NAKPKFREVRKKTFLDHFNRTGGDKNLHTLYSAVTGDN--FSGESVLEITLNYEQKSRRAVEEYCATLKKLFCVGLI---ALLGHAALKGDDEEEKLLQEWSEKMKVVQSKMIVVIEDCINSFPTQSELDAKRIVRDQSDKSNQQLADMLVEHLKKKYDWVCWSVRIFNSPTGL 284
[Close]
BLAST of Rapunzel vs. TrEMBL
Match: A0A3Q0T9T1 (EGF-like domain-containing protein OS=Amphilophus citrinellus OX=61819 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 3.340e-22
Identity = 88/304 (28.95%), Postives = 151/304 (49.67%), Query Frame = 2 
Query:   71 NFADESIKKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTV--LGALGPA-AMAISAVLSIVSMFLPKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKAL-------EDAQRENTKEGYQFFAKQYENS-FEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRELGGDN-QKFAEKIAQDLNNVVPQRSWLAVVYNDAS 946
            +  ++S  ++  VL  +K+  S   +V++ INP K+   + ++    +L P     +S+V+S+V  F+P+ED P LKA+K GF +VN KLD ++ +I +L   +E  S  + Y +    I N ++         E  QRE+ +   Q F   YEN+  E    N+Y  +T S+     N+  L K   KCN  ++ ++     SL+ KG+ +   Y   + F++ A+    +   K   + +   S +E C N+++ Y      +T + L  DN Q  A ++ Q L+      SW+ VVY DAS
Sbjct:   44 DLPEDSRDQADQVLNVAKDSLSLFKDVMENINPDKVVGVIKSLSNFASLAPGIGGVVSSVISMVLAFIPQED-PVLKAVKEGFAEVNRKLDSLSIQISDLATDVEWFSYVSVYSQDELRILNAWRKFNEFRESGELMQREDKQRLAQTFTNYYENTGAETGMTNLYHYLTVSSTSLSGNINELLKRKFKCNVSQIGKYNLYLSSLLWKGMVVNQFYWKLMGFNTAAKEAGHTQMFK--TVSEAQISTVEFCLNNYEQYMKNDVIETAKALSHDNKQDIAVQVKQVLDQKYSWYSWVVVVY-DAS 343
[Close]
BLAST of Rapunzel vs. TrEMBL
Match: A0A3Q2EDW5 (EGF-like domain-containing protein OS=Cyprinodon variegatus OX=28743 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 6.067e-16
Identity = 84/321 (26.17%), Postives = 156/321 (48.60%), Query Frame = 2 
Query:   47 SLVNVINCNFADESIKKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTV--LGALGPAAMAIS-AVLSIVSMFLPKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALEDAQREN-----TKEGYQF---FAKQYENS-FEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAY--IGYLNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRELGGDNQK-FAEKIAQDLNNVVPQRSWLAVVYNDASGFGEHT 964
            S +N+I      E+ +K+Q  L  +K+  S   +V+  +N  KL   +  +    +L P   A + +V+++V  F+P++D P L  +K GF +VN KLD ++ KI NL   +E  +  + Y +    I N +K   D    +      K+G      F + YENS  E +  N Y  +TG++     N++NL K  +KC+  ++ ++ +   SL+ KG+ +   Y  +  +N   K +  A+  K   +A + +    +E C  +++DY  +   +  ++L  DN+K  A+++ + L+N      W+ +V         HT
Sbjct:   31 STLNIIKRQLPPETREKTQKTLNVAKDSLSLFKDVVNMMNSEKLTTVMKGISRFASLAPGVAATAFSVVNMVLAFIPQDD-PVLNEVKKGFNEVNRKLDSLSVKISNLATDVEWFNYVSVYSRDEATILNAWKRFNDLHLHSNLVKRAKDGLTLSEKFIRYYENSGVESSVSNFYRYLTGTSISLHGNLINLLKKKLKCDINKMGRYNSYLSSLLWKGMVLNQFYWKMAGVNMKGKEDQPAQLLKKVSEAQLAE----VEFCLQNYEDYLKKDVVEIVKDLSPDNKKAIADQVKEALDNKYSWYCWVVLVIGKEKNHLLHT 346
[Close]
BLAST of Rapunzel vs. TrEMBL
Match: A0A3P9P6N7 (EGF-like domain-containing protein OS=Poecilia reticulata OX=8081 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.261e-15
Identity = 77/310 (24.84%), Postives = 145/310 (46.77%), Query Frame = 2 
Query:   53 VNVINCNFADESIKKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTV--LGALGPAAMAISAVLSIVSM---FLPKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKAL-------EDAQRENTKEGYQFFAKQYENS-FEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRELGGDNQKFAEKIAQDLNNVVPQRSWLAVVYNDA 943
            ++ +      ES++ ++  L  +KE  S   +V+ +++  KL A +  +    +  P   A+  V S+V+M   F+P+ED P L  +K GF +VN KLD ++ KI NL   +E  +  + Y +    I N ++         E    E T +  + F + YE+S  E +  N+Y  +T ++     N+ +L K   KC+   +S++     SL  KG+ +   Y       SKA    + +K   +A +    S +E C  ++K Y  +   +  ++ G D    A ++ + L+      +W+ VV++ +
Sbjct:   43 ISALRRELVSESMELTEKTLDVAKESLSLFKDVMGSMDSKKLSAVMKGISSFSSFAPGVGAV--VFSVVNMVLAFIPQED-PVLNEVKKGFAEVNQKLDSLSFKISNLAADVEWFNYASIYSRDELAIFNTWEKFGEFLHRTESQSPEETSKKIKIFTRYYESSGAESSVSNLYRYLTVNSTSLSENLNSLLKKKFKCDVYMISRYNLYLSSLFWKGMVLNQFYWKLTGVKSKAAEPGQMFKKVSEAQL----SAVEFCLTNYKQYLQKDVVEITKDQGSDQNSLALQVKEALDKKYSWYNWVVVVFDKS 345
[Close]
BLAST of Rapunzel vs. TrEMBL
Match: A0A3B4EJ69 (EGF-like domain-containing protein OS=Pygocentrus nattereri OX=42514 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 4.023e-15
Identity = 84/351 (23.93%), Postives = 168/351 (47.86%), Query Frame = 2 
Query:   83 ESIKKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTV--LGALGPAAMA-ISAVLSIVSMFLPKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALEDAQR---ENTKEGYQFFAKQYENS-FEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRELGG-----DNQKFAEKIAQDLNNVVPQRSWLAVVYNDASGFGEHTYSSYYETEKVWDQKDHDVTIFSTPRQEELTGSNLENLIKPINEQK 1099
            E+ +K++ VL ++    S LN+V+  I+   L + +  +    +L P   A IS++++ V +F+P+ED P L  ++ GF +VN+KLD ++++I +L   +E  +  + Y +    I N +    +  R   +N +   Q F   Y+++  E + + +Y  +TG T   G N+ +L +   KC+   ++++      L+ KGL ++  Y   ++ +S  EAK       + +L +   + +E C     D   +Q K   +++G      +    AEK+   L+      +W+ +VY+      ++ Y        + D+    VT       ++ TG N++N++K    QK
Sbjct:   46 ETREKTEKVLETATNSLSLLNDVMGKIDTNNLKSMMKNLANFASLAPGVGALISSIVNTVLVFIPQED-PVLTKLEQGFSEVNSKLDSLSTQISDLATDVEFFNYVSVYSQNEVRILNAWSKFVELNRHGFKNFQASAQTFKDYYKDTQTEASVDALYLYLTGRTMILGKNLNDLLRNKFKCDLNEINKYNFYLSGLLWKGLVLKEVYGKLIDSTSDKEAKHAQ---MLKSLYEVQRAAVEYC----IDNSEEQMKTDVKDIGTAHISEEKTAVAEKVKNALDKKYSWYNWVVLVYDKKQEEDKYIYVYDMTKIPISDKIIVAVTYTLKADSDKDTGENVKNIVKTCFRQK 388
[Close]
BLAST of Rapunzel vs. TrEMBL
Match: A0A3B5R579 (EGF-like domain-containing protein OS=Xiphophorus maculatus OX=8083 PE=4 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 7.193e-15
Identity = 76/300 (25.33%), Postives = 140/300 (46.67%), Query Frame = 2 
Query:   83 ESIKKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTV--LGALGPAAMAISAVLSIVSM---FLPKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKAL-------EDAQRENTKEGYQFFAKQYENS-FEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRELGGDNQKFAEKIAQDLNNVVPQRSWLAVVYNDA 943
            ES +K++  L  +KE  +   +V+ +++  KL   +  +    +  P   A+  V S+V+M   F+P++D P L  +K GF +VN KLD ++ KI NL   +E  +  + Y +    I N ++         E    E T +  + F   YE+S  E +  N+Y  +T  +     N+ +L K   KC+   +S++     SL  KG+ +   Y       SKA    + +K   +A +    S +E C  ++K Y  +   +  ++ G D    A ++ + L+N     +W+ VV++ +
Sbjct:   38 ESREKTEKTLNVAKESLTLFKDVMGSMDSKKLSDVMKGISSFASFAPGVGAV--VFSVVNMVLAFIPQDD-PVLSEVKKGFVEVNQKLDSLSIKISNLATDVEWFNYASVYSRDELTILNTFEKFGEFLHRTESQSPEETSKKTKIFTSYYESSGAESSVSNLYRYLTVDSTSLSENLNSLLKKKFKCDVYMISRYNLYLSSLFWKGMILNQFYWKLTGVKSKAAEPGQMFKKVSEAQL----SAVEFCLTNYKQYLKKDVVEITKDQGSDQNALALQVKEALDNKYSWYNWVVVVFDKS 330
[Close]
BLAST of Rapunzel vs. Ensembl Cavefish
Match: rpz4 (uncharacterized LOC103039774 [Source:NCBI gene;Acc:103039774])

HSP 1 Score: 50.0618 bits (118), Expect = 2.746e-6
Identity = 70/295 (23.73%), Postives = 125/295 (42.37%), Query Frame = 2 
Query:   92 KKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTVLGALGPAAMAISAVLSIVSMFLPKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALED---AQRENTKEGYQFFAKQYENSFEFATENI-YDAMTGSTGPFGFNMVNLYKTNVKCNPVR-LSQFCTMGISLINKGLTIEGAYIGY--LNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRE-LGGDNQKFAEKIAQDLNNVVPQRSWLAVVYNDASGF 952
            +K Q  +A  K+V   + EV +     +    ++++ G L P   AI+A   IV + L   +S E + +K  F++V  +L++++ +I  +   ++ S A AAY     N+ N ++   D   A+ +  +   + F + +  +      NI Y+A+TG    F    V     N +    R +  FC     L   GL    A +G+  L      E   + W  K+  +  + +++IE C NS+        K   R+     NQ+ A  I + L        W   V+N  +G 
Sbjct:    3 EKLQKYVADKKDVVETVMEVFE-----QGAEVVASIAGDLFPV-FAIAA--PIVKLALDNVESKEAEYMKEQFQRVRERLEVVSEEIQRINEEVKKSGADAAYFSVEENLTNQFRKYMDILNAKPKFREVKKKLFLEHFSKTGGDKNLNILYNAVTGDN--FSGESVLEISLNYEQKSRRAVEDFCARLKQLFCVGLI---ALMGHAALKGGEDEEGLLKDWADKMKEVQLKMNAVIEDCINSFPSQAEIDIKRLVRDNRDKPNQQLANMILEALKKKYDWIGWSIRVFNSPTGL 284
[Close]
BLAST of Rapunzel vs. Planmine SMEST
Match: SMESG000037569.1 (SMESG000037569.1)

HSP 1 Score: 894.419 bits (2310), Expect = 0.000e+0
Identity = 434/434 (100.00%), Postives = 434/434 (100.00%), Query Frame = 2 
Query:    2 FNKLETFVLIAICLVSLVNVINCNFADESIKKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTVLGALGPAAMAISAVLSIVSMFLPKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALEDAQRENTKEGYQFFAKQYENSFEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRELGGDNQKFAEKIAQDLNNVVPQRSWLAVVYNDASGFGEHTYSSYYETEKVWDQKDHDVTIFSTPRQEELTGSNLENLIKPINEQKNKIFNTNECGSSRLRNNEARRQVETIREKFSGGVVYAVFGDKRPGHAATGNNMIVSDQKWKCNNYTYR 1303
            FNKLETFVLIAICLVSLVNVINCNFADESIKKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTVLGALGPAAMAISAVLSIVSMFLPKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALEDAQRENTKEGYQFFAKQYENSFEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRELGGDNQKFAEKIAQDLNNVVPQRSWLAVVYNDASGFGEHTYSSYYETEKVWDQKDHDVTIFSTPRQEELTGSNLENLIKPINEQKNKIFNTNECGSSRLRNNEARRQVETIREKFSGGVVYAVFGDKRPGHAATGNNMIVSDQKWKCNNYTYR
Sbjct:    4 FNKLETFVLIAICLVSLVNVINCNFADESIKKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTVLGALGPAAMAISAVLSIVSMFLPKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALEDAQRENTKEGYQFFAKQYENSFEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRELGGDNQKFAEKIAQDLNNVVPQRSWLAVVYNDASGFGEHTYSSYYETEKVWDQKDHDVTIFSTPRQEELTGSNLENLIKPINEQKNKIFNTNECGSSRLRNNEARRQVETIREKFSGGVVYAVFGDKRPGHAATGNNMIVSDQKWKCNNYTYR 437
[Close]
BLAST of Rapunzel vs. Planmine SMEST
Match: SMESG000027656.1 (SMESG000027656.1)

HSP 1 Score: 606.29 bits (1562), Expect = 0.000e+0
Identity = 280/436 (64.22%), Postives = 356/436 (81.65%), Query Frame = 2 
Query:   20 FVLIAICLVSLVNVINCNFADESIKKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTVLGALGPAAMAISAVLSIVSMFLPKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALEDAQRENTKEGYQFFAKQYENSFEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRELGGDNQKFAEKIAQDLNNVVPQRSWLAVVYNDASGFGEHTYSSYYETEKVWDQKDHDVTIFSTPRQEELTGSNLENLIKPINEQKNKIFNTNECGSS--------RLRNNEARRQVETIREKFSGGVVYAVFGDKRPGHAATGNNMIVSDQKWKCNNYTYR 1303
            F+ IA CL SL +VI C++  ++IKK+QAVLASSK+VF+NL+E I + NPGKLFAKLS VLG+LGPAAMAISA+LSIV+MFLPKEDSPELKAIK GFEKVNNKLD I+SKIG+LENIIELSSARAAYVKTVT+INNNY+ L +A++ N+KEGY +FAKQYENSFEFAT N+YDAMTGSTGPFGFNMVNLYKT+V+CNPV+LSQFCTMG+ LI KGLT+EGAY+GY+NFS+KA+ K E+W  KI+ + KQCS++IE+CE++WK++ YQQ+K+T+REL GDNQ FAE++A+DL NV PQRSWL + YNDASGFG HTYS+YYE   +W++++HD ++FS P+  +LTGS+ +N +K IN QK  I   ++C  +        R  N +AR  V  IRE++  GVVYA+  D +PG AATGN +  ++QKW+C    +R
Sbjct:    7 FLFIA-CLFSLFSVIKCSYEADTIKKTQAVLASSKDVFANLSEFITSANPGKLFAKLSGVLGSLGPAAMAISAILSIVTMFLPKEDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSINNNYETLNEAKKSNSKEGYDYFAKQYENSFEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDNQAFAERLAKDLENVAPQRSWLTIAYNDASGFGPHTYSTYYEVHTIWNERNHDTSVFSVPKSPKLTGSDYDNFVKLINGQKEAIARVDDCKRTEFWLIKKDRYENEDARTIVNKIRERYMTGVVYAIRLDYKPGLAATGNTLYATEQKWQCTGEQFR 441
[Close]
BLAST of Rapunzel vs. Planmine SMEST
Match: SMESG000046431.1 (SMESG000046431.1)

HSP 1 Score: 452.21 bits (1162), Expect = 8.552e-157
Identity = 211/435 (48.51%), Postives = 308/435 (70.80%), Query Frame = 2 
Query:   26 LIAICLVSLVNVINCNFADESIKKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTVLGALGPAAMAISAVLSIVSMFL--PKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALEDAQRENTKEGYQFFAKQYENSFEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRELGGDNQKFAEKIAQDLNNVVPQRSWLAVVYNDASGFGEHTYSSYYETEKVWDQKDHDVTIFSTPRQEELTGSNLENLIKPINEQKNKIFNTNECGSS------RLRNNEARRQV-ETIREKFSGGVVYAVFGDKRPGHAATGNNMIVSDQKWKCNNYTYR 1303
            ++A+ L+ +  ++  N    S+   +  L S + + +N+  ++++  P ++F+K+S VL  LGP  M+ISA++S  + F   PKE+S ELKAI  GFEK+N++LD++T K+GNL+ ++E+S+ARAAYV+TV+ IN+ Y++L D  ++NTKE Y FFAKQYE S++FA  N+YDA+TGS+G FGFN++NLYK +VKCN V L +FC MGI LINKGL+IEGAY+G+++F++K EAK E+WK K+D + ++C  L+  CE SWK+Y Y+QF  T+ E+G DNQ FA K+A++L+ VVP RSWLAV YNDASGF +H YSSYYE EK+W++K HDVTIFSTP++E LTG +L NL+  I  QK ++     C           R  EA R + + IR+ +  G VY +     PG++ATGN +  ++QK+ C + TY+
Sbjct:   11 IVALLLLIVCYIVIANAGSTSLAGHKETLGSGEGLINNIKNLVKSGKPKEMFSKISGVLKKLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEEALTGGDLSNLVNLIEAQKQQVLKLQNCNGKYYWLVRHKRGIEAARAIADKIRDNYYRGTVYTINMRFSPGYSATGNTLYATEQKFHCGSETYK 445
[Close]
BLAST of Rapunzel vs. Planmine SMEST
Match: SMESG000027653.1 (SMESG000027653.1)

HSP 1 Score: 452.21 bits (1162), Expect = 8.552e-157
Identity = 211/435 (48.51%), Postives = 308/435 (70.80%), Query Frame = 2 
Query:   26 LIAICLVSLVNVINCNFADESIKKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTVLGALGPAAMAISAVLSIVSMFL--PKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALEDAQRENTKEGYQFFAKQYENSFEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRELGGDNQKFAEKIAQDLNNVVPQRSWLAVVYNDASGFGEHTYSSYYETEKVWDQKDHDVTIFSTPRQEELTGSNLENLIKPINEQKNKIFNTNECGSS------RLRNNEARRQV-ETIREKFSGGVVYAVFGDKRPGHAATGNNMIVSDQKWKCNNYTYR 1303
            ++A+ L+ +  ++  N    S+   +  L S + + +N+  ++++  P ++F+K+S VL  LGP  M+ISA++S  + F   PKE+S ELKAI  GFEK+N++LD++T K+GNL+ ++E+S+ARAAYV+TV+ IN+ Y++L D  ++NTKE Y FFAKQYE S++FA  N+YDA+TGS+G FGFN++NLYK +VKCN V L +FC MGI LINKGL+IEGAY+G+++F++K EAK E+WK K+D + ++C  L+  CE SWK+Y Y+QF  T+ E+G DNQ FA K+A++L+ VVP RSWLAV YNDASGF +H YSSYYE EK+W++K HDVTIFSTP++E LTG +L NL+  I  QK ++     C           R  EA R + + IR+ +  G VY +     PG++ATGN +  ++QK+ C + TY+
Sbjct:   11 IVALLLLIVCYIVIANAGSTSLAGHKETLGSGEGLINNIKNLVKSGKPKEMFSKISGVLKKLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEEALTGGDLSNLVNLIEAQKQQVLKLQNCNGKYYWLVRHKRGIEAARAIADKIRDNYYRGTVYTINMRFSPGYSATGNTLYATEQKFHCGSETYK 445
[Close]
BLAST of Rapunzel vs. Planmine SMEST
Match: SMESG000062792.1 (SMESG000062792.1)

HSP 1 Score: 88.5817 bits (218), Expect = 9.252e-19
Identity = 89/376 (23.67%), Postives = 161/376 (42.82%), Query Frame = 2 
Query:   26 LIAICLVSLVNVINCNFADESIKKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTVLGALGPAAMAISAVLSIVSMFLPKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNI---NNNYKALEDAQRENTKEGYQFFAKQYENSFEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYREL-----GGDNQKFAEKIAQDLNNVVPQRSWLAVVYNDASGFGEHTYSSYYETEKVWDQKDHDVTIFSTPRQEELTGSNLENLIKPINEQKNKIFNTNECG 1129
            ++ +CL SL+ +   + +   I +  + L  +KE+F+++ E          F K +  +   G   +      S V M   + +S EL  +K  F+ +N+K+D +  +   ++  I  +  +  Y    T I    +   ++ DA     +     F   Y+ ++  A + +Y++ T     F  N+V         +  ++ +F    I LI KGL +E  Y+   N +S  +  AE W  KI+    Q S+++E   NS K   + Q +    E      G  N+++ E+    L +    R W  VVYN  +G G++ Y S Y        + H++ + S  R +E    N  NLI  + +   + FNT   G
Sbjct:    7 ILCLCLSSLIPIYGLDTSPGKILEEASKLEKAKEIFNSIKE--------SDFVKTTQAISKWGLPLLKSIGYFSKVIMAQFETESKELTYMKEQFKIINDKMDTVDQQFDEVKKAINWNVVKVQYFNHETAIGLLQDMILSISDAPSNVRQMMMDRFRITYDQTYYNAADKLYESFTNPQRIFSENLVTASCQTFNFHRRKVEKFFLGNIQLILKGLEVESFYLTTKNLTSNQDYWAEQWNKKIN----QISAIMEETINSIKGNWHSQLQKDAEEFNVKNYGKGNKEYLEEFYNFLTDKFSWREWFLVVYNPITG-GDNHYVSVYGGIINLRSRGHNIAVAS--RDKEDNNFN-ANLISQVLDNLPRSFNTRGKG 366
The following BLAST results are available for this feature:
BLAST of Rapunzel vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rpz66.262e-824.31rapunzel 6 [Source:ZFIN;Acc:ZDB-GENE-170530-5][more]
rpz31.831e-623.41rapunzel 3 [Source:ZFIN;Acc:ZDB-GENE-030131-4784][more]
rpz43.262e-622.11rapunzel 4 [Source:ZFIN;Acc:ZDB-GENE-050417-472][more]
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BLAST of Rapunzel vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3Q0T9T13.340e-2228.95EGF-like domain-containing protein OS=Amphilophus ... [more]
A0A3Q2EDW56.067e-1626.17EGF-like domain-containing protein OS=Cyprinodon v... [more]
A0A3P9P6N71.261e-1524.84EGF-like domain-containing protein OS=Poecilia ret... [more]
A0A3B4EJ694.023e-1523.93EGF-like domain-containing protein OS=Pygocentrus ... [more]
A0A3B5R5797.193e-1525.33EGF-like domain-containing protein OS=Xiphophorus ... [more]
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BLAST of Rapunzel vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpz42.746e-623.73uncharacterized LOC103039774 [Source:NCBI gene;Acc... [more]
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BLAST of Rapunzel vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000037569.10.000e+0100.00SMESG000037569.1[more]
SMESG000027656.10.000e+064.22SMESG000027656.1[more]
SMESG000046431.18.552e-15748.51SMESG000046431.1[more]
SMESG000027653.18.552e-15748.51SMESG000027653.1[more]
SMESG000062792.19.252e-1923.67SMESG000062792.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012932 ID=SMED30012932|Name=Rapunzel|organism=Schmidtea mediterranea sexual|type=transcript|length=1366bp
GTTCAATAAATTAGAGACTTTTGTTTTAATTGCAATTTGTTTGGTCAGTT
TGGTCAATGTAATTAATTGCAATTTTGCCGACGAATCAATCAAGAAATCT
CAAGCTGTGCTCGCCTCGAGTAAAGAAGTCTTTTCGAATTTGAATGAAGT
AATTCAGGCAATAAATCCTGGCAAACTGTTTGCAAAGCTTAGTACAGTTC
TTGGAGCTTTGGGTCCCGCCGCAATGGCCATTTCTGCAGTTTTAAGTATT
GTTTCGATGTTCTTACCGAAAGAGGATAGTCCAGAACTCAAAGCCATCAA
AGCTGGATTTGAAAAAGTTAACAATAAATTGGACATAATAACCTCAAAAA
TTGGTAATCTTGAAAACATAATTGAGCTAAGTAGTGCCAGAGCTGCGTAC
GTCAAAACCGTCACAAATATCAACAATAATTACAAAGCTCTTGAAGATGC
CCAGAGAGAAAACACAAAGGAAGGATATCAATTCTTTGCTAAGCAATATG
AAAATTCATTTGAATTTGCAACGGAAAATATTTATGATGCAATGACTGGA
TCAACAGGACCTTTCGGATTCAATATGGTAAATTTATACAAAACAAATGT
AAAATGCAATCCAGTTCGTTTGAGTCAATTCTGCACAATGGGAATATCTT
TAATAAACAAAGGACTGACTATTGAAGGTGCCTATATTGGTTATCTTAAC
TTCTCCTCAAAGGCTGAAGCGAAAGCAGAATCATGGAAAGTGAAAATCGA
TGCATTGATGAAACAATGTTCCTCGCTGATTGAATCTTGTGAAAACAGTT
GGAAAGATTATGGCTATCAACAGTTCAAAGATACATACCGAGAACTTGGA
GGAGACAATCAAAAATTCGCAGAGAAAATTGCACAAGATTTGAACAATGT
TGTCCCTCAAAGAAGTTGGTTGGCAGTTGTATATAATGACGCCAGTGGAT
TTGGAGAACATACATATTCATCCTATTATGAAACAGAAAAGGTTTGGGAT
CAGAAAGATCACGACGTTACAATATTTTCAACACCGAGACAAGAGGAGTT
AACCGGATCAAATTTAGAGAATTTAATTAAACCAATTAACGAACAAAAAA
ACAAGATATTTAACACAAACGAATGTGGCTCTTCACGATTACGTAACAAT
GAAGCTCGAAGACAAGTGGAAACAATTCGAGAAAAGTTCTCAGGAGGAGT
TGTCTACGCAGTGTTTGGAGATAAAAGACCTGGTCACGCCGCAACTGGAA
ATAATATGATCGTTTCAGATCAAAAATGGAAATGCAATAATTACACTTAT
AGAATAATCATTTTCATTTAAACAAAAATTTAATGAATAAATAAATATTT
TAAATTCAAAAAAAAA
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protein sequence of SMED30012932-orf-1

>SMED30012932-orf-1 ID=SMED30012932-orf-1|Name=SMED30012932-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=440bp
FNKLETFVLIAICLVSLVNVINCNFADESIKKSQAVLASSKEVFSNLNEV
IQAINPGKLFAKLSTVLGALGPAAMAISAVLSIVSMFLPKEDSPELKAIK
AGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALEDA
QRENTKEGYQFFAKQYENSFEFATENIYDAMTGSTGPFGFNMVNLYKTNV
KCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEAKAESWKVKID
ALMKQCSSLIESCENSWKDYGYQQFKDTYRELGGDNQKFAEKIAQDLNNV
VPQRSWLAVVYNDASGFGEHTYSSYYETEKVWDQKDHDVTIFSTPRQEEL
TGSNLENLIKPINEQKNKIFNTNECGSSRLRNNEARRQVETIREKFSGGV
VYAVFGDKRPGHAATGNNMIVSDQKWKCNNYTYRIIIFI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000240body wall musculature
PLANA:0000418head
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR039051SE-CTX-like
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 235..255
NoneNo IPR availableSIGNALP_EUKSignalP-TMSignalP-TMcoord: 1..23
score: 0.521
NoneNo IPR availableTMHMMTMhelixcoord: 66..88
NoneNo IPR availableTMHMMTMhelixcoord: 4..26
IPR039051SE-cephalotoxin-likePANTHERPTHR40472FAMILY NOT NAMEDcoord: 32..428