NPHS1-2

Overview
NameNPHS1-2 (AKN21718.1)
Smed IDSMED30012922
Length (bp)433
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012922

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30012922h1SMcG0015241 SmedASXL_019305SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30012922h1SMcG0015241 SmedASXL_000724SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30012922h1SMcG0015241 SmedASXL_000300SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30012922h1SMcG0015241 SmedASXL_002095SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30012922h1SMcG0015241 SmedASXL_018902SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30012922h1SMcG0015241 dd_Smed_v4_16056_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012922h1SMcG0015241 dd_Smed_v4_16056_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30012922h1SMcG0015241 dd_Smed_v4_16056_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30012922h1SMcG0015241 dd_Smed_v4_16056_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30012922h1SMcG0015241 dd_Smed_v6_16056_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30012922h1SMcG0015241 OX_Smed_1.0.10682ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of NPHS1-2 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1])

HSP 1 Score: 53.5286 bits (127), Expect = 1.203e-8
Identity = 25/61 (40.98%), Postives = 36/61 (59.02%), Query Frame = 3 
Query:    9 RNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHLTFHRPN 191
            R++P  PR+SMTGD++ G   L+I +VT+ D G YECQV R    +  + R   L    P+
Sbjct:   70 RSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPS 130
[Close]
BLAST of NPHS1-2 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1])

HSP 1 Score: 53.5286 bits (127), Expect = 1.206e-8
Identity = 25/61 (40.98%), Postives = 36/61 (59.02%), Query Frame = 3 
Query:    9 RNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHLTFHRPN 191
            R++P  PR+SMTGD++ G   L+I +VT+ D G YECQV R    +  + R   L    P+
Sbjct:  115 RSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPS 175
[Close]
BLAST of NPHS1-2 vs. Ensembl Xenopus
Match: NPHS1 (NPHS1 nephrin [Source:NCBI gene;Acc:100494620])

HSP 1 Score: 48.9062 bits (115), Expect = 6.919e-7
Identity = 22/42 (52.38%), Postives = 27/42 (64.29%), Query Frame = 3 
Query:    6 NRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTR 131
            +RNIP   R+SMTGD   G+ +LRIE   + D  EY CQV R
Sbjct:   61 DRNIPGFLRYSMTGDPHKGEYNLRIERSELDDDAEYHCQVGR 102
[Close]
BLAST of NPHS1-2 vs. Ensembl Mouse
Match: Nphs1 (nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:1859637])

HSP 1 Score: 45.8246 bits (107), Expect = 5.285e-6
Identity = 22/46 (47.83%), Postives = 26/46 (56.52%), Query Frame = 3 
Query:    6 NRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYG 143
            N  IP  PR+S+ GD   G+  L IE   +SD  EYECQV R   G
Sbjct:   88 NPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELG 133
[Close]
BLAST of NPHS1-2 vs. Ensembl Mouse
Match: Nphs1 (nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:1859637])

HSP 1 Score: 45.8246 bits (107), Expect = 5.701e-6
Identity = 22/46 (47.83%), Postives = 26/46 (56.52%), Query Frame = 3 
Query:    6 NRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYG 143
            N  IP  PR+S+ GD   G+  L IE   +SD  EYECQV R   G
Sbjct:   74 NPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELG 119
[Close]
BLAST of NPHS1-2 vs. TrEMBL
Match: A0A0H3YKL8 (NPHS1-2 (Fragment) OS=Schmidtea mediterranea OX=79327 GN=NPHS1-2 PE=2 SV=1)

HSP 1 Score: 220.32 bits (560), Expect = 5.544e-72
Identity = 106/106 (100.00%), Postives = 106/106 (100.00%), Query Frame = 2 
Query:  116 MSSYSRKLRQKRIAQINPFDFPPSQPLIYENNNRQPSSTILITISAKMYDNHNKKLNLKCESKEGQPDPTFEWYHNSQMLKEQELLVKIIDYKTHSFLTISFTQLM 433
            MSSYSRKLRQKRIAQINPFDFPPSQPLIYENNNRQPSSTILITISAKMYDNHNKKLNLKCESKEGQPDPTFEWYHNSQMLKEQELLVKIIDYKTHSFLTISFTQLM
Sbjct:    1 MSSYSRKLRQKRIAQINPFDFPPSQPLIYENNNRQPSSTILITISAKMYDNHNKKLNLKCESKEGQPDPTFEWYHNSQMLKEQELLVKIIDYKTHSFLTISFTQLM 106
[Close]
BLAST of NPHS1-2 vs. TrEMBL
Match: A0A0H3YFQ1 (NPHS1-1 OS=Schmidtea mediterranea OX=79327 GN=NPHS1-1 PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 6.127e-13
Identity = 30/58 (51.72%), Postives = 38/58 (65.52%), Query Frame = 3 
Query:    3 FNRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHLT 176
            F+RNIP  PR  MTG+   GD SL IENV +SD+G YECQ++     +   RR+T LT
Sbjct:  100 FDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAA-SQPPRRRMTKLT 156

HSP 2 Score: 41.2022 bits (95), Expect = 6.127e-13
Identity = 24/59 (40.68%), Postives = 31/59 (52.54%), Query Frame = 2 
Query:  182 PSQPLIYENNNRQPSSTILITISAKMYDNHNKKLNLKCESKEGQPDPTFEWYHNSQMLK 358
            PS P+IY+N      +   I++S K  D  +  +NL C S  G P PTFEW  N   LK
Sbjct:  161 PSTPIIYQNGQSNKQN---ISVSIKK-DEFDSNINLTCVSLNGNPVPTFEWSVNRSPLK 215
[Close]
BLAST of NPHS1-2 vs. TrEMBL
Match: A0A2H1CX96 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_01106 PE=4 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 5.891e-12
Identity = 30/59 (50.85%), Postives = 36/59 (61.02%), Query Frame = 3 
Query:    3 FNRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHLTF 179
            + RNIP  PR+SM GD   G+  L I NVT  DAG YECQV+    G+  LRR T L+ 
Sbjct:   27 YKRNIPSWPRYSMQGDPRQGEHDLVITNVTHEDAGTYECQVS-PVAGQSPLRRQTTLSI 84

HSP 2 Score: 40.4318 bits (93), Expect = 5.891e-12
Identity = 21/60 (35.00%), Postives = 31/60 (51.67%), Query Frame = 2 
Query:  182 PSQPLIYENNNR-QPSSTILITISAKMYDNHNKKL-NLKCESKEGQPDPTFEWYHNSQML 355
            PSQP+I   +   QP+    +T+  +      + L  L C+S  G P P FEW+HN  +L
Sbjct:   88 PSQPVIVAPSGEPQPTPNGRLTVPLRHSTPGERNLLRLSCQSHGGNPPPIFEWFHNGLVL 147
[Close]
BLAST of NPHS1-2 vs. TrEMBL
Match: A0A4E0RLR1 (Nephrin OS=Fasciola hepatica OX=6192 GN=D915_000783 PE=4 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 1.858e-11
Identity = 30/58 (51.72%), Postives = 36/58 (62.07%), Query Frame = 3 
Query:    3 FNRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHLT 176
            + RNIP  PR+SM GD   G+  L I NVT  DAG YECQV+    G+  LRR T L+
Sbjct:  167 YKRNIPSWPRYSMQGDPRQGEHDLVITNVTHEDAGTYECQVSP-VAGQSPLRRQTTLS 223

HSP 2 Score: 38.891 bits (89), Expect = 1.858e-11
Identity = 21/62 (33.87%), Postives = 32/62 (51.61%), Query Frame = 2 
Query:  182 PSQPLIYENNNR-QPSSTILITISAKMYDNHNKKL-NLKCESKEGQPDPTFEWYHNSQMLKE 361
            PSQP+I   +   QP+    +T+  +      + L  L C+S  G P P FEW+HN  +L +
Sbjct:  228 PSQPVIVAPSGEPQPTPNGRLTVPLRHSTPGERNLLRLSCQSHGGNPPPIFEWFHNGLVLNK 289
[Close]
BLAST of NPHS1-2 vs. TrEMBL
Match: A0A4S2M8S8 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_001836 PE=4 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 2.357e-11
Identity = 28/59 (47.46%), Postives = 37/59 (62.71%), Query Frame = 3 
Query:    3 FNRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHLTF 179
            + R+IP  PR+S+ GD   G+  L I NVT SDAG +ECQV+  + G   LRR T L+ 
Sbjct:   89 YVRSIPNWPRYSIQGDARLGNHDLLIRNVTKSDAGSFECQVSPAD-GHPPLRRQTTLSI 146

HSP 2 Score: 40.0466 bits (92), Expect = 2.357e-11
Identity = 20/69 (28.99%), Postives = 35/69 (50.72%), Query Frame = 2 
Query:  182 PSQPLIYENNNRQ---PSSTILI---TISAKMYDNHNKKLNLKCESKEGQPDPTFEWYHNSQMLKEQEL 370
            PSQP++   +      P+  +++    +++         L L CES+ G P PTFEW+HN   +K + +
Sbjct:  150 PSQPVVIAPSGEPQPLPNGQLIVPRHAVNSSTTSEMQNYLRLICESRGGVPPPTFEWFHNGISVKPERV 218
[Close]
BLAST of NPHS1-2 vs. Ensembl Cavefish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1])

HSP 1 Score: 52.7582 bits (125), Expect = 1.885e-8
Identity = 24/60 (40.00%), Postives = 34/60 (56.67%), Query Frame = 3 
Query:    9 RNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHLTFHRP 188
            R++P  PR+SMTGD++ G   L+IE+V + D   YECQV R    +  + R   L    P
Sbjct:   86 RSLPGHPRYSMTGDQDRGQYHLQIEDVRLEDDSPYECQVGRSKTSRPIVSRTVWLNVQIP 145
[Close]
BLAST of NPHS1-2 vs. Ensembl Cavefish
Match: ENSAMXT00000035639.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001167.1:20064:29865:-1 gene:ENSAMXG00000041886.1 transcript:ENSAMXT00000035639.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 44.669 bits (104), Expect = 2.747e-6
Identity = 20/50 (40.00%), Postives = 33/50 (66.00%), Query Frame = 3 
Query:   21 FKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTH 170
            ++ R S+ GD + G+ SL++EN+T++D GEY C  +RG      L R++H
Sbjct:   90 YRGRASLVGDLQKGNVSLKLENLTVADRGEYVCLFSRG-----PLFRVSH 134
[Close]
BLAST of NPHS1-2 vs. Planmine SMEST
Match: SMESG000021277.1 (SMESG000021277.1)

HSP 1 Score: 179.104 bits (453), Expect = 9.011e-53
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 2 
Query:  179 PPSQPLIYENNNRQPSSTILITISAKMYDNHNKKLNLKCESKEGQPDPTFEWYHNSQMLKEQELLVKIIDYKTHSFLTISFTQLM 433
            PPSQPLIYENNNRQPSSTILITISAKMYDNHNKKLNLKCESKEGQPDPTFEWYHNSQMLKEQELLVKIIDYKTHSFLTISFTQLM
Sbjct:  175 PPSQPLIYENNNRQPSSTILITISAKMYDNHNKKLNLKCESKEGQPDPTFEWYHNSQMLKEQELLVKIIDYKTHSFLTISFTQLM 259

HSP 2 Score: 126.331 bits (316), Expect = 2.672e-34
Identity = 63/77 (81.82%), Postives = 64/77 (83.12%), Query Frame = 3 
Query:    3 FNRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHLTFHRPNR*YMKTITDNQARQ 233
            FNRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHLT   P       I +N  RQ
Sbjct:  114 FNRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHLTVLVPPS--QPLIYENNNRQ 188
[Close]
BLAST of NPHS1-2 vs. Planmine SMEST
Match: SMESG000003359.1 (SMESG000003359.1)

HSP 1 Score: 60.4622 bits (145), Expect = 2.795e-16
Identity = 30/58 (51.72%), Postives = 38/58 (65.52%), Query Frame = 3 
Query:    3 FNRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHLT 176
            F+RNIP  PR  MTG+   GD SL IENV +SD+G YECQ++     +   RR+T LT
Sbjct:  100 FDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAA-SQPPRRRMTKLT 156

HSP 2 Score: 40.817 bits (94), Expect = 2.795e-16
Identity = 24/59 (40.68%), Postives = 31/59 (52.54%), Query Frame = 2 
Query:  182 PSQPLIYENNNRQPSSTILITISAKMYDNHNKKLNLKCESKEGQPDPTFEWYHNSQMLK 358
            PS P+IY+N      +   I++S K  D  +  +NL C S  G P PTFEW  N   LK
Sbjct:  161 PSTPIIYQNGQSNKQN---ISVSIKK-DEFDSNINLTCVSLNGNPVPTFEWSVNRSPLK 215
[Close]
BLAST of NPHS1-2 vs. Planmine SMEST
Match: SMESG000003359.1 (SMESG000003359.1)

HSP 1 Score: 60.4622 bits (145), Expect = 2.960e-16
Identity = 30/58 (51.72%), Postives = 38/58 (65.52%), Query Frame = 3 
Query:    3 FNRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHLT 176
            F+RNIP  PR  MTG+   GD SL IENV +SD+G YECQ++     +   RR+T LT
Sbjct:   95 FDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAA-SQPPRRRMTKLT 151

HSP 2 Score: 41.2022 bits (95), Expect = 2.960e-16
Identity = 24/59 (40.68%), Postives = 31/59 (52.54%), Query Frame = 2 
Query:  182 PSQPLIYENNNRQPSSTILITISAKMYDNHNKKLNLKCESKEGQPDPTFEWYHNSQMLK 358
            PS P+IY+N      +   I++S K  D  +  +NL C S  G P PTFEW  N   LK
Sbjct:  156 PSTPIIYQNGQSNKQN---ISVSIKK-DEFDSNINLTCVSLNGNPVPTFEWSVNRSPLK 210
[Close]
BLAST of NPHS1-2 vs. Planmine SMEST
Match: SMESG000003359.1 (SMESG000003359.1)

HSP 1 Score: 60.4622 bits (145), Expect = 3.005e-16
Identity = 30/58 (51.72%), Postives = 38/58 (65.52%), Query Frame = 3 
Query:    3 FNRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHLT 176
            F+RNIP  PR  MTG+   GD SL IENV +SD+G YECQ++     +   RR+T LT
Sbjct:   95 FDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAA-SQPPRRRMTKLT 151

HSP 2 Score: 41.2022 bits (95), Expect = 3.005e-16
Identity = 24/59 (40.68%), Postives = 31/59 (52.54%), Query Frame = 2 
Query:  182 PSQPLIYENNNRQPSSTILITISAKMYDNHNKKLNLKCESKEGQPDPTFEWYHNSQMLK 358
            PS P+IY+N      +   I++S K  D  +  +NL C S  G P PTFEW  N   LK
Sbjct:  156 PSTPIIYQNGQSNKQN---ISVSIKK-DEFDSNINLTCVSLNGNPVPTFEWSVNRSPLK 210
[Close]
BLAST of NPHS1-2 vs. Planmine SMEST
Match: SMESG000003549.1 (SMESG000003549.1)

HSP 1 Score: 51.6026 bits (122), Expect = 3.012e-11
Identity = 29/68 (42.65%), Postives = 39/68 (57.35%), Query Frame = 3 
Query:    3 FNRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHL-TFHRPNR*YM 203
            F+R IP KPRF +TGD    +  L I  +  SD G+YECQV    +    LRR THL  + +P+  Y+
Sbjct:   86 FDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKH-HPLLRRRTHLFVYKKPSFPYI 152

HSP 2 Score: 32.7278 bits (73), Expect = 3.012e-11
Identity = 20/59 (33.90%), Postives = 30/59 (50.85%), Query Frame = 2 
Query:  182 PSQPLIYENNNRQPSSTILITISAKMYDNHNKKLNLKCESKEGQPDPTFEWYHNSQMLK 358
            PS P I  N+    +  ILI       D   K ++L+C SK G P PT+ W  +++ L+
Sbjct:  147 PSFPYIVYNSKNVSNMDILI-------DQQKKCIHLECISKHGFPIPTYSWIVDNKFLE 198
The following BLAST results are available for this feature:
BLAST of NPHS1-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NPHS1-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NPHS1-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NPHS1-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
nphs11.203e-840.98NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:... [more]
nphs11.206e-840.98NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:... [more]
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BLAST of NPHS1-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
NPHS16.919e-752.38NPHS1 nephrin [Source:NCBI gene;Acc:100494620][more]
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BLAST of NPHS1-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Nphs15.285e-647.83nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:18... [more]
Nphs15.701e-647.83nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:18... [more]
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BLAST of NPHS1-2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NPHS1-2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0H3YKL85.544e-72100.00NPHS1-2 (Fragment) OS=Schmidtea mediterranea OX=79... [more]
A0A0H3YFQ16.127e-1351.72NPHS1-1 OS=Schmidtea mediterranea OX=79327 GN=NPHS... [more]
A0A2H1CX965.891e-1250.85Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A4E0RLR11.858e-1151.72Nephrin OS=Fasciola hepatica OX=6192 GN=D915_00078... [more]
A0A4S2M8S82.357e-1147.46Uncharacterized protein OS=Opisthorchis felineus O... [more]
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BLAST of NPHS1-2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
nphs11.885e-840.00NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:... [more]
ENSAMXT00000035639.12.747e-640.00pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of NPHS1-2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NPHS1-2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NPHS1-2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NPHS1-2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NPHS1-2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000021277.19.011e-53100.00SMESG000021277.1[more]
SMESG000003359.12.795e-1651.72SMESG000003359.1[more]
SMESG000003359.12.960e-1651.72SMESG000003359.1[more]
SMESG000003359.13.005e-1651.72SMESG000003359.1[more]
SMESG000003549.13.012e-1142.65SMESG000003549.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30012922
GenBankAKN21718.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012922 ID=SMED30012922|Name=NPHS1-2|organism=Schmidtea mediterranea sexual|type=transcript|length=433bp
GATTCAATAGGAACATTCCATTCAAGCCTCGGTTTTCAATGACTGGAGAT
GAAGAAGCAGGAGATCAAAGTTTAAGAATTGAAAACGTAACAATATCCGA
TGCTGGAGAATATGAATGTCAAGTTACTCGAGGAAACTACGGCAAAAAAG
AATTGCGCAGATTAACCCATTTGACTTTCCACCGTCCCAACCGCTGATAT
ATGAAAACAATAACAGACAACCAAGCTCGACAATTCTAATAACGATATCG
GCGAAAATGTACGACAATCACAACAAAAAGCTTAATCTGAAATGTGAATC
CAAAGAAGGTCAACCGGATCCAACCTTTGAATGGTATCACAATTCCCAAA
TGTTGAAAGAACAAGAACTTTTAGTTAAAATAATCGACTACAAAACTCAT
AGTTTTTTGACAATCAGTTTCACTCAGTTAATG
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protein sequence of SMED30012922-orf-1

>SMED30012922-orf-1 ID=SMED30012922-orf-1|Name=SMED30012922-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=106bp
MSSYSRKLRQKRIAQINPFDFPPSQPLIYENNNRQPSSTILITISAKMYD
NHNKKLNLKCESKEGQPDPTFEWYHNSQMLKEQELLVKIIDYKTHSFLTI
SFTQLM
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000140anterior region of the whole animal
PLANA:0000418head
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR036179Ig-like_dom_sf
IPR013783Ig-like_fold
IPR013106Ig_V-set
IPR007110Ig-like_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 21..105
e-value: 6.5E-7
score: 31.5
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 36..106
score: 7.649
NoneNo IPR availableCDDcd00096Igcoord: 56..101
e-value: 5.84966E-4
score: 33.7549
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 36..104