SMED30012893

Overview
NameSMED30012893
Smed IDSMED30012893
Length (bp)3020
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30012893 (SMED30012893) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30012893 (SMED30012893) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30012893 (SMED30012893) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30012893 (SMED30012893) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012893

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30012893SMESG000038297.1 SMESG000038295.1 SMESG000035869.1 SmedASXL_017422SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30012893 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002571:28418..31104 -11523
v31.008274:25641..28434 -11510
Homology
[Close]
BLAST of SMED30012893 vs. Planmine SMEST
Match: SMESG000038297.1 (SMESG000038297.1)

HSP 1 Score: 776.548 bits (2004), Expect = 0.000e+0
Identity = 410/422 (97.16%), Postives = 414/422 (98.10%), Query Frame = 2 
Query:  374 MFTIFGVFQFIQLNCRKSPLILPKFRYAIPVCLVLCILSEIMIVNQFVKINHDIRNIPKSKEHFTNQLINQINLSIDNTKSAFVESFKMKKYELFSLLDQAVNDAIRIVNKTSKIDSHSSKQDFGIFTNNNKFIRIMKSANLHVNSTHQMRYALIKLARKCDVNFFETRLLCLLFKNECHSIFDELKKLKNIFEIRDKSNKRFTITFGKIVPLIKEIAEIFNVSHFLLTPVQLYNEGSVRIASTVDKIINKHFNEILSKARHFIKPFSDEVLIKYQTEIDNITIFIFLIHFVMLVLIILSILQKVNNSLKFNLYFTDVFNFANCCICLFYLILFSVALTFQWXXXXXXXXXXXXXXXXXXNIIVSNRNRITLKIYLIGMSILVFGVAGTSVLLNSQTEVCRYLADIRQSSKIADQFIDSFLD 1639
            MFTIFGVFQFIQLNCRKSPLILPKFRYAIPVCLVLCILSEIMIVNQFVKINHDIRNI KSKEHFTNQLINQINLSIDNTKSAFVESFKMKKYELFSLLDQAVNDAIRIVNKTSKIDSHSSKQDFGIFTNNNKFIR MK ANLHVNSTHQMRYALIKLARKCDVNFFETRLLCLLFKNECHS+FDELKK KNIFEIRDKSNKRFTITFGKIVPLIKEIAEIFNVSHFLLTPVQLY+EGSVRIASTVDKIINKHFNEILSKARHFIKPFSDEVLIKYQTEI+NITIFIFLIHFVMLVLIILSILQKVNNSLKFN YFTDVFNFANCCICLFYLILFS+ALTFQWHFI IIIIVLHIIFCVTINIIVSNRNRITLKIYLIGMSILVF VAGTSVLLNSQTEVCRYLADI QSSKIADQFIDSFLD
Sbjct:    1 MFTIFGVFQFIQLNCRKSPLILPKFRYAIPVCLVLCILSEIMIVNQFVKINHDIRNIAKSKEHFTNQLINQINLSIDNTKSAFVESFKMKKYELFSLLDQAVNDAIRIVNKTSKIDSHSSKQDFGIFTNNNKFIRTMKLANLHVNSTHQMRYALIKLARKCDVNFFETRLLCLLFKNECHSMFDELKKFKNIFEIRDKSNKRFTITFGKIVPLIKEIAEIFNVSHFLLTPVQLYDEGSVRIASTVDKIINKHFNEILSKARHFIKPFSDEVLIKYQTEINNITIFIFLIHFVMLVLIILSILQKVNNSLKFNRYFTDVFNFANCCICLFYLILFSLALTFQWHFINIIIIVLHIIFCVTINIIVSNRNRITLKIYLIGMSILVFSVAGTSVLLNSQTEVCRYLADISQSSKIADQFIDSFLD 422

HSP 2 Score: 312.768 bits (800), Expect = 1.276e-126
Identity = 164/208 (78.85%), Postives = 173/208 (83.17%), Query Frame = 3 
Query: 1506 VCQYLCSVSQEHRYC*ILKQKSVDISQIFANRQKLPINLLIHFLIPDKILSIPKLNIKNPKGILTTVIRQCPAKRILFPSLKLYRPINTTAIIFSEFFRQDILNPGLSKVNQSIYENFEDILKIYKKVFDSKIHLLELLKYFPQS*EKTDFSFKKLNQSIEMLNYRRNKVLEDYINQKLKFESVKFYKCFDFIGNSTIFKGNENVLFD 2129
            VC+YL  +SQ  +    +  + +D       +  L  N      IPDKILSIPKLNIKNPKGILTTVIRQCPAKRILFPSLKLYRPINTTAIIFSEFF QDILNPGLSKVN+SIYENFEDILKIY KVFDSKIHLLELLKYFPQS EKTDFSFKKLNQSIEMLNYRRNKV EDYINQ+LKFESVKF KCFDFI NSTIFK NENVLFD
Sbjct:  399 VCRYLADISQSSK----IADQFIDSFLDLQGKSILAKN------IPDKILSIPKLNIKNPKGILTTVIRQCPAKRILFPSLKLYRPINTTAIIFSEFFHQDILNPGLSKVNESIYENFEDILKIYNKVFDSKIHLLELLKYFPQSGEKTDFSFKKLNQSIEMLNYRRNKVFEDYINQRLKFESVKFDKCFDFISNSTIFKENENVLFD 596

HSP 3 Score: 162.155 bits (409), Expect = 1.276e-126
Identity = 81/94 (86.17%), Postives = 85/94 (90.43%), Query Frame = 1 
Query: 2125 LIKEFVVIMNNMKESIESEREKFFTNFLQSAVEPVKVYANQLITLLEDDTLSRVIPCNELSTFYLETVNRLCTNQLGNFLVLMIFETLTFTTSV 2406
            LIKEFVVIMNNMKESIESEREKFFTNFLQSAVEPVKVYANQLITLLEDDTLSRVIPCNELSTFYLETVN LCTN +G+FLV M+   L FT  +
Sbjct:  607 LIKEFVVIMNNMKESIESEREKFFTNFLQSAVEPVKVYANQLITLLEDDTLSRVIPCNELSTFYLETVNGLCTNHIGSFLVFMMSVNLYFTFGI 700
[Close]
BLAST of SMED30012893 vs. Planmine SMEST
Match: SMESG000035869.1 (SMESG000035869.1)

HSP 1 Score: 311.227 bits (796), Expect = 4.136e-126
Identity = 163/208 (78.37%), Postives = 172/208 (82.69%), Query Frame = 3 
Query: 1506 VCQYLCSVSQEHRYC*ILKQKSVDISQIFANRQKLPINLLIHFLIPDKILSIPKLNIKNPKGILTTVIRQCPAKRILFPSLKLYRPINTTAIIFSEFFRQDILNPGLSKVNQSIYENFEDILKIYKKVFDSKIHLLELLKYFPQS*EKTDFSFKKLNQSIEMLNYRRNKVLEDYINQKLKFESVKFYKCFDFIGNSTIFKGNENVLFD 2129
            VC+YL  +SQ  +    +  + +D       +  L  N      IPDKILSIPKLNIKNPKGIL TVIRQCPAKRILFPSLKLYRPINTTAIIFSEFF QDILNPGLSKVN+SIYENFEDILKIY KVFDSKIHLLELLKYFPQS EKTDFSFKKLNQSIEMLNYRRNKV EDYINQ+LKFESVKF KCFDFI NSTIFK NENVLFD
Sbjct:   21 VCRYLADISQSSK----IADQFIDSFLDLQGKSILAKN------IPDKILSIPKLNIKNPKGILATVIRQCPAKRILFPSLKLYRPINTTAIIFSEFFHQDILNPGLSKVNESIYENFEDILKIYNKVFDSKIHLLELLKYFPQSGEKTDFSFKKLNQSIEMLNYRRNKVFEDYINQRLKFESVKFDKCFDFISNSTIFKENENVLFD 218

HSP 2 Score: 161.77 bits (408), Expect = 4.136e-126
Identity = 79/94 (84.04%), Postives = 84/94 (89.36%), Query Frame = 1 
Query: 2125 LIKEFVVIMNNMKESIESEREKFFTNFLQSAVEPVKVYANQLITLLEDDTLSRVIPCNELSTFYLETVNRLCTNQLGNFLVLMIFETLTFTTSV 2406
            LIKEFVVIMNNMKESIESEREKFFTNFLQSA+EPVKVYANQL+TLLEDDTLSRVIPCNELSTFYLETVN LCTN +G FLV M+   L FT  +
Sbjct:  229 LIKEFVVIMNNMKESIESEREKFFTNFLQSAIEPVKVYANQLVTLLEDDTLSRVIPCNELSTFYLETVNGLCTNHIGGFLVFMMSVNLYFTIGI 322

HSP 3 Score: 86.2705 bits (212), Expect = 8.069e-18
Identity = 42/44 (95.45%), Postives = 42/44 (95.45%), Query Frame = 2 
Query: 1508 MSILVFGVAGTSVLLNSQTEVCRYLADIRQSSKIADQFIDSFLD 1639
            MSILVF VAGTSVLLNSQTEVCRYLADI QSSKIADQFIDSFLD
Sbjct:    1 MSILVFSVAGTSVLLNSQTEVCRYLADISQSSKIADQFIDSFLD 44
[Close]
BLAST of SMED30012893 vs. Planmine SMEST
Match: SMESG000038295.1 (SMESG000038295.1)

HSP 1 Score: 337.035 bits (863), Expect = 2.237e-102
Identity = 192/472 (40.68%), Postives = 287/472 (60.81%), Query Frame = 2 
Query:  227 MFQKLLIETSMILQPNINGGLFQYLTSTDKNLLFMLIGKFTIFYIVLYIMFTIFGVFQFIQLNCRKSPLILPKFRYAIPVCLVLCILSEIMIVNQFVKINHDIRNIPKSKEHFTNQLINQINLSIDNTKSAFVESFKMKKYELFSLLDQAVNDAIRIVNKTSKIDS-HSSKQDFGIFTNNNKFIRIMKSANLHVNSTHQMRYALIKLARKCDVNFFETRLLCLLFKNECHSIFDELKKLKNIFEIRDKSNKRFTITFGKIVPLIKEIAEIFNVSHFLLTPVQLYNEGSVRIASTVDKIINKHFNEILSKARHFIKPFSDEVLIKYQTEIDNITIFIFLIHFVMLVLIILSILQKVNNSLKFNLYFTDVFNFANCCICLFYLILFSVALTFQWXXXXXXXXXXXXXXXXXXNIIVSNRNRITLKIYLIGMSILVFGVAGTSVLLNSQTEVCRYLADIRQSSKIADQFIDSFLD 1639
            M+Q   IE SMILQ N NGGLF+YL++ ++ L  ML       Y++L  +F IF     +    R+  L  PKFR  IP+ ++ C+    ++  Q  +IN D+  I KS +++TN++I Q++LSI+NTK +F+++ K KK+ELF L+D+A+ D ++I NK+ K ++  +S+  F +F  ++  + I+K A+++++ST++MR AL+KL R C V+ F+ RLLC L++ EC   FDEL+K++  F+IRD +NKRFTITFGKIVPLIKE+A+ FN+S FL+TP+Q Y+ G  R+   +DK+INK  NE+L K  H  +P   + L K+Q  +      I +++ + +VL+++S+      +    + +  ++N    CI  F                                             Y +G++ILV  VAGTSVLLNSQTEVCRYLADI QSSKIADQFIDSFLD
Sbjct:    1 MWQNKFIEISMILQSNANGGLFKYLSNVNEMLNIMLWSWLINIYLILLPIF-IFTPLCLLFPPIRRY-LFFPKFRITIPLLVIFCVTCGTVVFVQLSEINQDVGKIQKSSDYYTNKVIQQLSLSINNTKLSFIKALKKKKFELFPLVDKAITDVVKIANKSLKGNNLKNSEFSFELFGKSDVVLDILKRADVYIDSTYEMRNALVKLPRICLVHLFKNRLLCYLYRRECQEFFDELEKMQKFFKIRDLANKRFTITFGKIVPLIKEVAKSFNISQFLMTPIQFYDYGVDRMDGILDKVINKQINEVLMKNLHQNRPIISKYLQKFQNYVKYFLYSIVIMNVIFIVLVLISVGLNCVTTSNMKVIYPLIWN----CIHFF--------------------------------------------AYFVGLTILVLFVAGTSVLLNSQTEVCRYLADISQSSKIADQFIDSFLD 422

HSP 2 Score: 315.849 bits (808), Expect = 1.726e-94
Identity = 192/329 (58.36%), Postives = 214/329 (65.05%), Query Frame = 3 
Query: 1506 VCQYLCSVSQEHRYC*ILKQKSVDISQIFANRQKLPINLLIHFLIPDKILSIPKLNIKNPKGILTTVIRQCPAKRILFPSLKLYRPINTTAIIFSEFFRQDILNPGLSKVNQSIYENFEDILKIYKKVFDSKIHLLELLKYFPQS*EKTDFSFKKLNQSIEMLNYRRNKVLEDYINQKLKFESVKFYKCFDFIGNSTIFKGNENVLFDKGICCDHEQYE-------------RKY*E*KRE-IFHKFPSISCRTGKSLRQSI-----DNAFRR*YIIPSDSM**IVHFLPRNRQQIVYKSAGKFSSPYDF*NFDFYNISAILLVIMMYY 2435
            VC+YL  +SQ  +    +  + +D       +  L  N      IPDKILSIPKLNIKNPKGILTTVIRQCPAKRILFPSLKLYRPINTTAIIFSEFF QDILNPGLSKVN+SIYENFEDILKIY KVFDSKIHLLELLKYFPQS EKTDFSFKKLNQSIEMLNYRRNKV EDYINQ+LKFESVKF KCFDFI NSTIFK NENVLFD  +  D + +E             ++  E +RE  F  F   +    K     +     D+   R  +IP + +     F       +     G F     F    F  ISAILLVIMM Y
Sbjct:  399 VCRYLADISQSSK----IADQFIDSFLDLQGKSILAKN------IPDKILSIPKLNIKNPKGILTTVIRQCPAKRILFPSLKLYRPINTTAIIFSEFFHQDILNPGLSKVNESIYENFEDILKIYNKVFDSKIHLLELLKYFPQSGEKTDFSFKKLNQSIEMLNYRRNKVFEDYINQRLKFESVKFDKCFDFISNSTIFKENENVLFD--LVEDMKYFENLIKEFVVIMNNMKESIESEREKFFTNFLQSAVEPVKVYANQLITLLEDDTLSR--VIPCNEL---STFYLETVNGLCTNQLGNFLVLMIFETLTFTTISAILLVIMMSY 710

HSP 3 Score: 181.415 bits (459), Expect = 3.078e-47
Identity = 91/92 (98.91%), Postives = 91/92 (98.91%), Query Frame = 1 
Query: 2125 LIKEFVVIMNNMKESIESEREKFFTNFLQSAVEPVKVYANQLITLLEDDTLSRVIPCNELSTFYLETVNRLCTNQLGNFLVLMIFETLTFTT 2400
            LIKEFVVIMNNMKESIESEREKFFTNFLQSAVEPVKVYANQLITLLEDDTLSRVIPCNELSTFYLETVN LCTNQLGNFLVLMIFETLTFTT
Sbjct:  607 LIKEFVVIMNNMKESIESEREKFFTNFLQSAVEPVKVYANQLITLLEDDTLSRVIPCNELSTFYLETVNGLCTNQLGNFLVLMIFETLTFTT 698
The following BLAST results are available for this feature:
BLAST of SMED30012893 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012893 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012893 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012893 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012893 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012893 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012893 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012893 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012893 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012893 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012893 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012893 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012893 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012893 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000038297.11.276e-12697.16SMESG000038297.1[more]
SMESG000035869.14.136e-12678.37SMESG000035869.1[more]
SMESG000038295.12.237e-10240.68SMESG000038295.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012893 ID=SMED30012893|Name=SMED30012893|organism=Schmidtea mediterranea sexual|type=transcript|length=3020bp
TCTTATTAGTTTGAAAAGCGTAAATATTTGTTTAATCGATTTATTTTTAA
GAGAAATTAAAATGTTTTATAAACTTAAAAATTAAAAAAGATTTTTAAAA
ATTCGATTTTAATATGTAGAATTTTTTAATTCGATTCGAATTTAATTTGC
ATTAAAAAATAACTTAATTTTAAAATCATTGTTTCTATTTCTAAAATAAT
TAAAAGTTGTTAATAGTTTATCAAAAATGTTTCAGAAATTACTTATTGAA
ACTTCTATGATTTTACAGCCAAATATTAATGGCGGATTGTTTCAATACTT
AACTAGTACTGACAAAAATTTGTTATTTATGTTAATTGGTAAATTTACTA
TATTTTACATCGTTTTGTATATTATGTTTACAATTTTCGGAGTTTTTCAA
TTTATTCAATTAAATTGTAGAAAATCTCCCCTTATTTTACCGAAATTTCG
ATATGCTATACCAGTTTGTTTAGTATTGTGTATATTAAGCGAAATAATGA
TAGTTAATCAATTTGTTAAAATAAATCATGATATTAGGAATATACCAAAA
TCGAAAGAGCATTTTACAAATCAGTTGATAAATCAAATTAATTTATCGAT
AGATAATACAAAATCAGCCTTTGTTGAATCATTTAAAATGAAAAAATACG
AATTATTTTCACTACTCGATCAAGCTGTTAATGATGCTATCAGAATTGTT
AATAAAACTTCCAAAATAGATTCACACTCATCTAAGCAGGATTTTGGAAT
TTTTACTAATAATAACAAATTTATTAGAATAATGAAGTCAGCAAATTTAC
ATGTTAATTCCACTCATCAAATGCGATACGCGCTAATTAAGCTTGCAAGA
AAATGCGATGTTAATTTTTTTGAAACTCGACTACTTTGCCTTCTTTTTAA
AAATGAATGCCATTCTATTTTTGATGAACTGAAAAAGTTAAAAAATATAT
TCGAAATCCGTGATAAAAGTAATAAACGTTTCACAATTACATTTGGTAAA
ATCGTTCCTCTAATTAAAGAAATAGCAGAGATATTTAACGTTAGCCATTT
TCTTCTGACACCTGTTCAACTCTATAATGAAGGATCTGTTAGGATTGCTA
GTACAGTCGATAAAATTATCAATAAGCATTTCAATGAAATATTATCTAAG
GCTCGTCATTTTATTAAACCATTTTCAGACGAAGTTCTAATAAAATATCA
AACAGAAATTGATAATATTACGATATTTATTTTTCTGATACATTTTGTTA
TGTTAGTTTTGATTATATTGTCTATTTTGCAGAAAGTCAATAACAGTCTA
AAATTCAATCTATATTTCACAGATGTTTTCAATTTTGCTAACTGTTGCAT
CTGTTTGTTCTATTTGATTTTATTTTCAGTAGCTTTAACTTTTCAATGGC
ATTTTATTATTATAATTATTATTGTATTACATATTATATTTTGCGTTACT
ATTAATATAATCGTATCCAATAGAAATCGTATTACTTTAAAAATATATTT
AATCGGTATGTCAATACTTGTGTTCGGTGTCGCAGGAACATCGGTATTGC
TGAATTCTCAAACAGAAGTCTGTCGATATCTCGCAGATATTCGCCAATCG
TCAAAAATTGCCGATCAATTTATTGATTCATTTCTTGATCCCAGATAAAA
TCCTATCAATTCCAAAGCTCAACATAAAGAATCCAAAAGGAATTTTAACC
ACGGTAATTCGTCAATGTCCTGCTAAACGAATTCTTTTTCCGAGTTTGAA
ATTATATCGGCCGATCAATACAACTGCAATCATATTTTCGGAGTTCTTCC
GCCAAGATATTCTAAATCCCGGCTTATCGAAAGTCAATCAAAGTATTTAT
GAGAATTTCGAAGATATTTTGAAAATTTACAAAAAAGTTTTCGATTCGAA
AATCCATCTGTTGGAATTATTGAAATATTTTCCTCAGAGTTGAGAAAAAA
CCGATTTTTCATTCAAGAAACTCAACCAATCTATCGAAATGTTGAATTAT
CGGAGAAATAAAGTACTTGAAGATTATATCAATCAGAAATTGAAATTTGA
AAGCGTGAAATTCTATAAATGTTTCGATTTTATTGGTAATTCTACGATAT
TTAAGGGAAACGAAAATGTTCTATTTGATAAAGGAATTTGTTGTGATCAT
GAACAATATGAAAGAAAGTATTGAGAGTGAAAGAGAGAAATTTTTCACAA
ATTTCCTTCAATCAGCTGTCGAACCGGTAAAAGTTTACGCCAATCAATTG
ATAACGCTTTTAGAAGATGATACATTATCCCGAGTGATTCCATGTAATGA
ATTGTCCACTTTTTACCTAGAAACCGTCAACAGATTGTGTACAAATCAGC
TGGGAAATTTTCTAGTCCTTATGATTTTTGAAACTTTGACTTTTACAACA
TCAGTGCAATATTGCTTGTAATAATGATGTATTATTGATTTTTTTTAAGA
AGTTTTTACAAAACTACCTAGATACTTTCCCAAACTATTTATGTAAATAC
ATGATATCTATTTTCTGTTTTATTTGTAACTGTTTTATATTTGAGCACAT
TTAATCCGATATTTTCAGATGTTTTATCGATTTTACATATTATATTTTAG
AGCATTTAACAAATTGTTACTTGGACTTGTATTGCAGTTTTGGTGGTTTC
ATACTTGTGAAGTTTTCATGTTTATATTTATAGTTTATTCGGATTGAATA
CACCAAAGTGCTTTTATAAACTTTTTTTGTTACATAAATTTATGTCAAGC
CGCTTGTAATAATGCAAAAGTTATTTTATTTTATCTAAATTTTGTAAAGT
TATTTTAAGAGTAATTTTAGTGTTTTTTATTTAATAGGAATTTTTGAAAA
TTTACGACGGAAAATAAAGGTGAGATGTTTATTTTAATATGCAAACAACA
CTCTTGTTAATAACAGAACATTTGTGTTATTTTTGAAATGTAGTTTAACT
CTAGGGTATAACATTTTATTTTTAATTTATAATGAAAACAAAATTTTATT
AAATTAACATTTGTTTACGG
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protein sequence of SMED30012893-orf-1

>SMED30012893-orf-1 ID=SMED30012893-orf-1|Name=SMED30012893-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=474bp
MFQKLLIETSMILQPNINGGLFQYLTSTDKNLLFMLIGKFTIFYIVLYIM
FTIFGVFQFIQLNCRKSPLILPKFRYAIPVCLVLCILSEIMIVNQFVKIN
HDIRNIPKSKEHFTNQLINQINLSIDNTKSAFVESFKMKKYELFSLLDQA
VNDAIRIVNKTSKIDSHSSKQDFGIFTNNNKFIRIMKSANLHVNSTHQMR
YALIKLARKCDVNFFETRLLCLLFKNECHSIFDELKKLKNIFEIRDKSNK
RFTITFGKIVPLIKEIAEIFNVSHFLLTPVQLYNEGSVRIASTVDKIINK
HFNEILSKARHFIKPFSDEVLIKYQTEIDNITIFIFLIHFVMLVLIILSI
LQKVNNSLKFNLYFTDVFNFANCCICLFYLILFSVALTFQWHFIIIIIIV
LHIIFCVTINIIVSNRNRITLKIYLIGMSILVFGVAGTSVLLNSQTEVCR
YLADIRQSSKIADQFIDSFLDPR*
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protein sequence of SMED30012893-orf-2

>SMED30012893-orf-2 ID=SMED30012893-orf-2|Name=SMED30012893-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=102bp
MFYLIKEFVVIMNNMKESIESEREKFFTNFLQSAVEPVKVYANQLITLLE
DDTLSRVIPCNELSTFYLETVNRLCTNQLGNFLVLMIFETLTFTTSVQYC
L*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 79..101
NoneNo IPR availableTMHMMTMhelixcoord: 50..72
NoneNo IPR availableTMHMMTMhelixcoord: 27..46