Spondin-1
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30012850 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Homology
BLAST of Spondin-1 vs. Ensembl Human
Match: SPON1 (spondin 1 [Source:HGNC Symbol;Acc:HGNC:11252]) HSP 1 Score: 220.32 bits (560), Expect = 9.695e-60 Identity = 160/549 (29.14%), Postives = 259/549 (47.18%), Query Frame = 2
Query: 350 DRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHK---MIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPM-VKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEET--IKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIK---------PKHNWECTKMNAKQVKEFDE-----MNSNKNGNSQFDGSSLTKKRRHINK--KSPLD---------------DPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
D ++PG SYR+ L+ A FR F++ A E + + E H I DE F+ N CP + + T ++ WIAP C+ +A +V+ + ++ G L K +C E++ FD +T + +CCACG A Y++TF G WS+KTHPKD+P++ + HW+ +IG +H+ +Y ++ GG AS+ V+ + G ++++ +++ + +K+ W + + S SV+RT H SFLTMMGPSPDW G++A D+C +C W DL P+DAG +G TY E+ ++ IP + IR + + Q PF + VARV+ ++I P + + A + K+ D+ + SN + S S+ K +R + K+ LD P ++ C S W W+ CS++CG+G R R + + + LP E+ C + C S+ C + W W C+ATC M G ++R RM
Sbjct: 70 DFYKPGTSYRVTLSAAPPSY-FRGFTLIALRENREGDKEEDHAGTFQIIDEEETQFMSN---------CPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLC---------EQDST------FDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRAN--HWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPA-WQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTY--ESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEEKDEDDTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSISCGMGMRSRERYVKQFPEDGSVCTLPTEETEKCTVNE-ECSPSS-CLMTEWGEWDECSATCGM-GMKKRHRMI 585 HSP 2 Score: 53.9138 bits (128), Expect = 1.370e-6 Identity = 34/90 (37.78%), Postives = 44/90 (48.89%), Query Frame = 2
Query: 1598 TFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
T C+ SPWSEW+ CSVTCG G R R + A+ C L + C + D C L WS+W CN +C G+ R+RM
Sbjct: 612 TIPCLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNE-DLEQVEKCMLPECPID----CELTEWSQWSECNKSC-GKGHVIRTRMI 695
BLAST of Spondin-1 vs. Ensembl Human
Match: SPON2 (spondin 2 [Source:HGNC Symbol;Acc:HGNC:11253]) HSP 1 Score: 108.227 bits (269), Expect = 8.089e-25 Identity = 82/303 (27.06%), Postives = 129/303 (42.57%), Query Frame = 2
Query: 800 CCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPEN--SDRQDI------PDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCG-IGTEMRSR 1681
C A +A Y +TF G WS+ PK +P + W++L+GA H+ DY ++ S ++ G++ L ++ E L+S S + V R H SF+ + PSPDW GV ++D+C D W++ +DL+P+DAG +GFT+ N + QD P N+ Y + A P+ARV ++ + + A + D N D +S+ ++PLD C S WS W C CG +GT+ R+R
Sbjct: 35 CSARALAKYSITFTGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEI--EAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGD-RWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFYYPR--LKALPPIARVTLVRL--RQSPRAFIPPAPVLPSRD--------NEIVDSASV--------PETPLD-----------CEVSLWSSWGLCGGHCGRLGTKSRTR 303
BLAST of Spondin-1 vs. Ensembl Human
Match: SPON2 (spondin 2 [Source:HGNC Symbol;Acc:HGNC:11253]) HSP 1 Score: 108.227 bits (269), Expect = 8.089e-25 Identity = 82/303 (27.06%), Postives = 129/303 (42.57%), Query Frame = 2
Query: 800 CCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPEN--SDRQDI------PDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCG-IGTEMRSR 1681
C A +A Y +TF G WS+ PK +P + W++L+GA H+ DY ++ S ++ G++ L ++ E L+S S + V R H SF+ + PSPDW GV ++D+C D W++ +DL+P+DAG +GFT+ N + QD P N+ Y + A P+ARV ++ + + A + D N D +S+ ++PLD C S WS W C CG +GT+ R+R
Sbjct: 35 CSARALAKYSITFTGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEI--EAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGD-RWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFYYPR--LKALPPIARVTLVRL--RQSPRAFIPPAPVLPSRD--------NEIVDSASV--------PETPLD-----------CEVSLWSSWGLCGGHCGRLGTKSRTR 303
BLAST of Spondin-1 vs. Ensembl Human
Match: SPON2 (spondin 2 [Source:HGNC Symbol;Acc:HGNC:11253]) HSP 1 Score: 108.227 bits (269), Expect = 8.089e-25 Identity = 82/303 (27.06%), Postives = 129/303 (42.57%), Query Frame = 2
Query: 800 CCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPEN--SDRQDI------PDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCG-IGTEMRSR 1681
C A +A Y +TF G WS+ PK +P + W++L+GA H+ DY ++ S ++ G++ L ++ E L+S S + V R H SF+ + PSPDW GV ++D+C D W++ +DL+P+DAG +GFT+ N + QD P N+ Y + A P+ARV ++ + + A + D N D +S+ ++PLD C S WS W C CG +GT+ R+R
Sbjct: 35 CSARALAKYSITFTGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEI--EAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGD-RWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFYYPR--LKALPPIARVTLVRL--RQSPRAFIPPAPVLPSRD--------NEIVDSASV--------PETPLD-----------CEVSLWSSWGLCGGHCGRLGTKSRTR 303
BLAST of Spondin-1 vs. Ensembl Human
Match: TFPI (tissue factor pathway inhibitor [Source:HGNC Symbol;Acc:HGNC:11760]) HSP 1 Score: 67.3958 bits (163), Expect = 2.511e-11 Identity = 42/141 (29.79%), Postives = 66/141 (46.81%), Query Frame = 2
Query: 2009 CGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANK-----CRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKAC 2416
C + +YFY+ C F+Y GC G++NNF + C+ C N + L + S + + K+P++ C G + + R+YY++ G+CR FKY+GC GN NNF S C++AC
Sbjct: 134 CRGYITRYFYNNQTKQCERFKYGGCLGNMNNFETLEECKNICEDGPNGFQV--DNYGTQLNAVNNSLTPQSTKVPSLFEFHGPSWCLTPADRGLCRAN-----ENRFYYNSVIGKCRPFKYSGCGGNENNFTSKQECLRAC 267 HSP 2 Score: 55.8398 bits (133), Expect = 1.616e-7 Identity = 42/138 (30.43%), Postives = 61/138 (44.20%), Query Frame = 2
Query: 2015 KPVMK-YFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANK---CRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKAC 2416
K +MK +F++ C F Y GC+G+ N F S C+K C ++ N+++ K K C LE G C G + RY+Y+N+ +C +FKY GC GN NNF + C C
Sbjct: 64 KAIMKRFFFNIFTRQCEEFIYGGCEGNQNRFESLEECKKMC---------------------TRDNANRIIKTTLQQEKPDFCFLEEDPG--ICRGYI---TRYFYNNQTKQCERFKYGGCLGNMNNFETLEECKNIC 175
BLAST of Spondin-1 vs. Ensembl Celegans
Match: spon-1 (SPONdin (Extracellular matrix glycoprotein) family [Source:UniProtKB/TrEMBL;Acc:Q19305]) HSP 1 Score: 255.758 bits (652), Expect = 1.483e-72 Identity = 206/800 (25.75%), Postives = 340/800 (42.50%), Query Frame = 2
Query: 344 MADRFEPGESYRIILNN--AMYGMD-FRDFSIFAAYEMEDSLQMESHKMIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFL---TYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPF----TANVP-VARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCR-HLPLLESRTCEGRKRSCD----FSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF-----------ARFEEKEKCLKNKERRFKIFEEKRCK----KDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMP-----------DQI--DFMKTAPIKA-----CQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSE 2596
++ F PGE Y++ + Y + FR F + + +E S G + + K G R G + L T V W AP C+ F A V++ ++ + L +C K +I KP D+ + CCAC +A Y + F G+WSK THPKD+P + + H+T+++G++H+ +Y ++ GG ++ ++ I +G++ K + + ++S G+W D ++S F+ VN+ HHF S TM GPSPDWC G+++V++C+ DCTW ++ T +L PFDAG +G TY N + + +PI I T PF + +P +A+VI ++ + + + K D + + + + K RR C+ + W W+ CS TCG G +RSR + K ++ H E + C + C+ FS+ C + SW WG C+ C HG + R+R F E K+ C+ + + CK D + C C EG+ + + +FY + C+ N K +C M + + NS++ + A +C G RY+Y++ +C +F +TGC GN N F + C + C+P +Q+ DF T C+VS+W+ WG CS +CG G++ + R V++ A NGG C E + E
Sbjct: 59 ISKGFVPGEIYKVSIRGWRTQYTVKTFRGFVVSSLFEDNTSA-------------GSWQVVKGHGDARISPGCRQSGVSHANLKSKTSVHMMWKAPEVSSGCVVFRASVIETKYIWFTEAEGLTVKLCI----------QKGTQILKPVDDPSAT---CCACDIAQYDLEFTGIWSKNTHPKDYPTLEHLTHFTDMLGSSHSSNYSLWTIGGISTDGMKEIAEWGNT--YKAEAEAKAKASEVRSLMKVKGLWFPDVQGTTKSQFV-VNKYHHFVSLATMFGPSPDWCVGLSSVNLCLPDCTWAEERTFELQPFDAGTDSGPTYMSPN-EPTEPREPIHWITTKLNPLSPFYNSKSDTIPTLAKVILRR---------KNVTSSECKSDDILKAEAHNITNTSEDEEYKDRRE-------------------CMMTQWEPWSLCSATCGKGIRIRSRVYVFPIKAQVFHCHRQTTEKQFCNAKINECENSEAFSSKCQVSSWGSWGECSVQC-GHGMRSRNRTFLNPATKSGDCSVDLERKDICVGENGDDCNVTPDPLCKTTAWSDWSPCSAS--CDEGVRVRTRL------FFYSEHEKRCMHV----------NLQEKDTC-----------------VMQSCRRFIEINSEEICQEDKQAGQC--------------AGNFPRYWYNHEKTQCERFIFTGCKGNRNQFETEEECKQICLPGYEKSKSLIPNNQLLEDFGNTEVDDGGERVDCEVSKWTAWGSCSVSCGRGKKSRSRHVVKLARNGGHQCSEHLMQE 750 HSP 2 Score: 53.5286 bits (127), Expect = 6.217e-7 Identity = 29/78 (37.18%), Postives = 40/78 (51.28%), Query Frame = 2
Query: 1607 CITSPWSEWTKCSVTCGIGTEMRSRDMLK---NAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATC 1831
C S W+ W CSV+CG G + RSR ++K N + H L++ C R R C C + WSRW PC+ +C
Sbjct: 706 CEVSKWTAWGSCSVSCGRGKKSRSRHVVKLARNGGHQCSEH--LMQELQC--RLRPCPVKLTCQVGPWSRWSPCSVSC 779
BLAST of Spondin-1 vs. Ensembl Celegans
Match: ZC84.1 (pep chromosome:WBcel235:III:9199450:9206285:-1 gene:WBGene00013846.1 transcript:ZC84.1.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZC84.1) HSP 1 Score: 83.1889 bits (204), Expect = 6.433e-16 Identity = 67/219 (30.59%), Postives = 97/219 (44.29%), Query Frame = 2
Query: 1829 CEMHGYQ---QRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDP--VIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALP-------QWRKDKMAHL-----------QFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKAC 2416
CEM+ + +R EE ++C N + CK D +C P IC++ L +G C + V +Y+Y+A C FEY GC+G+ NNF S M C+ C NA+P Q KD + ++ + + PT A C L G+ C G +YYY+ + C F+Y GCDGNSNNF + +C C
Sbjct: 480 CEMYCARLQCERGSPLRIGEEAQRCQNNAQ----CPSSHECKADQGVCCPRKQTICAQPLRIGD-CTENVKRYWYNARTRQCQMFEYTGCQGNDNNFDSIMDCQNFCK---NAIPEPKCIQGQAYKDMFGNFVTCSNGMGCPANYECYFDGSQWGCCPTKAFTCSLNTD-SGIQCGA--GSTFKYYYNPQTQNCESFQYNGCDGNSNNFANRDACESYC 687
BLAST of Spondin-1 vs. Ensembl Celegans
Match: F30H5.3 (pep chromosome:WBcel235:III:505241:512217:-1 gene:WBGene00017937.1 transcript:F30H5.3.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F30H5.3) HSP 1 Score: 80.8777 bits (198), Expect = 3.792e-15 Identity = 62/212 (29.25%), Postives = 92/212 (43.40%), Query Frame = 2
Query: 1937 RCKKDTALC--DPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTC--LAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMP----------------TMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKAC---------MPDQIDFMKTAPIKACQVSEWSH 2485
C+ D +C P ICS+ L +G C + V +Y+Y+A +C F+Y GC+G+ NNF + + C+ TC + PQ K + SNS P T A C L + G+ C G RYYY+++ C ++Y GCDGNSNNF + C C P++ +F + QV +H
Sbjct: 547 ECQSDHNVCCPRPQAICSQPLRLGD-CKQSVRRYWYNAVTRACEIFDYTGCQGNDNNFETLLECQNTCENIIPEPQCPQGDAYKDYQGNYYVCSNSGAGNSCPVNYECYFDGYVWGCCPTKAYTCTLS-PHKGVTCGS--GSSYRYYYNSQTQECESYQYNGCDGNSNNFATREDCEGYCGVGGCPNGGTPERNEFGQLMVCSTTQVCPGTH 754 HSP 2 Score: 55.4546 bits (132), Expect = 1.968e-7 Identity = 42/138 (30.43%), Postives = 56/138 (40.58%), Query Frame = 2
Query: 2027 KYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTC--LAAVNALP------QWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKAC 2416
+Y+YDA + C+ F Y G G+ NNF S CE C L P R A + SD P C ++ G +C ++ RY+Y+ C F YTGC GN NNF + C C
Sbjct: 481 RYYYDAEQGRCIAFTYNGALGNYNNFKSSADCELFCAKLQCKYGTPLKIGSSNQRCSASADCPSTHECQSDHNVCCPRPQAICSQPLRLG--DCKQSV---RRYWYNAVTRACEIFDYTGCQGNDNNFETLLECQNTC 613
BLAST of Spondin-1 vs. Ensembl Celegans
Match: ZC84.6 (pep chromosome:WBcel235:III:9192983:9199507:-1 gene:WBGene00013849.1 transcript:ZC84.6.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZC84.6) HSP 1 Score: 78.9518 bits (193), Expect = 1.162e-14 Identity = 60/185 (32.43%), Postives = 84/185 (45.41%), Query Frame = 2
Query: 1937 RCKKDTALCDPVI--ICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWR-------KDKMAHL--------------QFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDI--RYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKAC 2416
CK D A+C P + IC++ L +G+ C + V +Y+Y AA C FEY GC+G+ NNF + + C+ C NA P+ R KD F+ + + PT A C L G T GP + +Y+Y+ + C F+Y GCDGNSN F S C C
Sbjct: 440 ECKMDQAVCCPRMQTICTQPLRVGN-CDRSVRRYWYSAATRECQSFEYTGCQGNDNNFETLVDCQTFCR---NAAPEPRCPQGQAYKDNQGKFVTCTTNRQSSSCPANFECYFDGNMHGCCPTKAFTCSLSPSPG-----KTCGPGVSFKYHYNPQTQECESFEYLGCDGNSNTFASRAECENYC 615 HSP 2 Score: 73.1738 bits (178), Expect = 8.620e-13 Identity = 52/169 (30.77%), Postives = 67/169 (39.64%), Query Frame = 2
Query: 1970 VIICSEGLIMGSSCGKPV-MKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTC-LAAVNALPQWRKDKMAHLQFQSQSNSD-----------------KRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACM 2419
CS G +CG V KY Y+ C FEYLGC G+ N F S+ CE C + +D LQ S+ + + PT C L + G C G + RYY++ +C F Y GCDGN NNF S + C CM
Sbjct: 560 AFTCSLSPSPGKTCGPGVSFKYHYNPQTQECESFEYLGCDGNSNTFASRAECENYCGVGGCANGGSPLRDSNGALQSCSERDGGCPSSHECYGVSLGPDMMSYRCCPTKTYICGLPPQQGSSLCSGGLTVVTRYYFNIVTRKCSPFVYNGCDGNPNNFASLNQCNNFCM 728 HSP 3 Score: 67.0106 bits (162), Expect = 5.663e-11 Identity = 46/138 (33.33%), Postives = 60/138 (43.48%), Query Frame = 2
Query: 2027 KYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTC--LAAVNALPQWRKDKMAHLQFQSQSNSDKRKKM------PTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKAC 2416
++FYD C PF YLG G+ NNF S++ CE C L P D + S KM P M C ++ G NCD ++ RY+Y C+ F+YTGC GN NNF + C C
Sbjct: 374 RFFYDPQSGKCSPFTYLGAGGNYNNFLSRIDCELYCARLQCDRGNPLRIGDVTQSCASNNDCPSSHECKMDQAVCCPRMQTICTQPLRVG--NCDRSV---RRYWYSAATRECQSFEYTGCQGNDNNFETLVDCQTFC 506
BLAST of Spondin-1 vs. Ensembl Celegans
Match: Y43F8B.3 (pep chromosome:WBcel235:V:19503108:19519950:1 gene:WBGene00012814.1 transcript:Y43F8B.3e.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y43F8B.3) HSP 1 Score: 75.0998 bits (183), Expect = 2.043e-13 Identity = 68/270 (25.19%), Postives = 103/270 (38.15%), Query Frame = 2
Query: 1832 EMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEG--------------LIMGSSCGKPV---------MKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVN------ALPQWRKDKM-------AHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQI----DFM----------KTAPIKACQVSEWSHWG 2491
E G + S F E EK + KI E C P +C G + G CG+P+ +++++ C+PF Y G KG+ NNF +K C++TC N ALP A++ +N D P+ + C L + G G D R+YY+ + G C+QF Y+G GN NNF + +C + C P+ F+ +A C ++ W H G
Sbjct: 626 EYRGMKGNSNNFLTRENCEKRCPVFQNPCKIGEPHIQNNQYMQCSPQQVCPGGHYCHVGTEANYCCKALGGDPCGQPLDRGVGGSQLSRWYWNQQSQCCLPFSYCGQKGTQNNFLTKQDCDRTCYVFTNPCNQPIALPATLCSGTGSSDTCGANMWCHIGANQDSTVCCPSEGDPCSLPLARG----SGNQFMD-RFYYNQQTGSCQQFTYSGLHGNQNNFLTQQACEEQCGPNPCFEGRPFVGADGRTQTCSASANFNTCPLNHWCHIG 890 HSP 2 Score: 63.929 bits (154), Expect = 4.513e-10 Identity = 55/206 (26.70%), Postives = 86/206 (41.75%), Query Frame = 2
Query: 1979 CSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTC------------LAAVNALP-QWRKDKMAHLQFQSQSNSDKRKKM--PTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSC-------IKACMPDQIDFM--------KTAPIKACQVSEWSHWGPCSTT 2506
C E + G + +++YDA++ C+ F YLG KG+ NNF SK CE TC + V P Q + + D + + + C L VK G N + R++Y++ +C+ F YTG GN NNF C I AC + + +A + +C ++ W H GP ++T
Sbjct: 281 CEEEMTQGEG-PSALTRFYYDASQRKCLAFNYLGLKGNRNNFQSKEHCESTCPVWTNPCAIGQPIMTVGQKPFQCHQGATCSTGYFCHLGYDDATTVCCQSEGDPCSLVVKEGSGNHHLS-----RWFYNSNTRQCQPFTYTGQGGNENNFLLREHCEATCPVWINACPSGEPYLLPNGKPQPCDSANVNSCPLTHWCHPGPDAST 480 HSP 3 Score: 62.3882 bits (150), Expect = 1.471e-9 Identity = 48/187 (25.67%), Postives = 75/187 (40.11%), Query Frame = 2
Query: 2021 VMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAH---------------LQFQSQSNSDKRKKM--PTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQ-------------IDFMKTAPIKACQVSEWSHWG 2491
+ ++FY++ C PF Y G G+ NNF + CE TC +NA P + + L D M P A+ C + G +G + R+Y++ ++ C +F + G GNSNNF+S C KAC Q + + K+C +W H+G
Sbjct: 398 LSRWFYNSNTRQCQPFTYTGQGGNENNFLLREHCEATCPVWINACPSGEPYLLPNGKPQPCDSANVNSCPLTHWCHPGPDASTTMCCPGKADPCTQTLAQG----EGPLS-VARFYFNAQSRTCDEFMFRGLKGNSNNFKSQEDCEKACPVQQNPCPITMSSLKHSAKLVPCSATKSCPSQQWCHYG 579 HSP 4 Score: 60.8474 bits (146), Expect = 3.878e-9 Identity = 67/242 (27.69%), Postives = 92/242 (38.02%), Query Frame = 2
Query: 1943 KKDTALCDPV--IICSEGLIMGSSCGKPVM-KYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMP-------------------TMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMP--------------DQIDFMKTAPIKACQVSEWSHWG--PCSTTCGVGERFKWRSVLRPA 2554
+D+ +C P CS L GS G M +++Y+ SC F Y G G+ NNF ++ +CE+ C N + R A + Q+ S S P N C L + G +G D R+YYD + CR F Y G GN NNF S +C +C P Q+ F +C V+ W H G P +T C G L P
Sbjct: 787 NQDSTVCCPSEGDPCSLPLARGS--GNQFMDRFYYNQQTGSCQQFTYSGLHGNQNNFLTQQACEEQC--GPNPCFEGRPFVGADGRTQTCSASANFNTCPLNHWCHIGSDLSTTVCCPGASTNACNLPMSTG----EGNARLD-RFYYDQQTKTCRPFVYNGLKGNQNNFISLRACQLSCQPLDNPCIGQPATTAAGQVLFCSITNKDSCPVNFWCHIGATPETTVCCPGATNPCSVPLAPG 1019 HSP 5 Score: 56.6102 bits (135), Expect = 9.794e-8 Identity = 44/155 (28.39%), Postives = 66/155 (42.58%), Query Frame = 2
Query: 2027 KYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVN-ALPQWRKDKMAHLQFQSQSNSDK---------------RKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMK 2443
+++YD +C PF Y G KG+ NNF S +C+ +C N + Q + F S +N D P N C + + G G G RYYY+ + +C F+Y G GN NNF + C + C P+Q+ +
Sbjct: 922 RFYYDQQTKTCRPFVYNGLKGNQNNFISLRACQLSCQPLDNPCIGQPATTAAGQVLFCSITNKDSCPVNFWCHIGATPETTVCCPGATNPCSVPLAPG----TGNAG-LARYYYNPDDRQCLPFQYNGKRGNQNNFENQADCERTC-PEQLCLLS 1070 HSP 6 Score: 54.299 bits (129), Expect = 4.919e-7 Identity = 41/142 (28.87%), Postives = 62/142 (43.66%), Query Frame = 2
Query: 2021 VMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNAL----PQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCD-----GTMGPDI-RYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKAC 2416
++ +D + CVPFEY G KG+ NNF ++ +CEK C N P + ++ Q + T AN C + GG C G G + R+Y++ ++ C F Y G G NNF + C + C
Sbjct: 610 ATRWAFDGSARKCVPFEYRGMKGNSNNFLTRENCEKRCPVFQNPCKIGEPHIQNNQYMQCSPQQVCPGGHYCHVGTEANYCCKAL--GGDPCGQPLDRGVGGSQLSRWYWNQQSQCCLPFSYCGQKGTQNNFLTKQDCDRTC 749
BLAST of Spondin-1 vs. Ensembl Fly
Match: fat-spondin (gene:FBgn0026721 transcript:FBtr0086975) HSP 1 Score: 234.572 bits (597), Expect = 2.749e-65 Identity = 208/852 (24.41%), Postives = 342/852 (40.14%), Query Frame = 2
Query: 188 ILWMLLCLHWMIQESFAMNTRTNCFKMHKKTYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF-IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKT------------ELC-RHLPLLESRTCEGRKRSCD-----------------FSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKK------DTALCDPVIICSE---GLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDF-MKTAPIKACQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPE-LFLSEPCFATTC 2617
+L L+ + W+ R H + + D +++++ D + + PG++Y ++L + + + F+ F + P +G F + + + +C N + D++ T V+ W+AP + C+ A V + + G L VIC K ++ KECCAC A Y F G+WS +THPKD+P + H++++IGA+H ++ + A+ +S+ +G L+ + P L++ +G+W + + Q+ S V+R H S ++M GPSPDW G++ +D+C DC+WK+ DLFP+DAG +G +Y NS+ Q P R T M +P P +++ P K+ ++ K ++N + +F L + ++ + D T A C +S W+ CSV+CG G MRSR L A E+C +P + + R D + C WS W C+A+C + G R+R F +++C I E+ +C + L DP S+ S+CG+ V + L L N P K SC + + + K N+++ K + A C GT +RY YD +N C F Y GC GN NNF + + C+ C + + + +AC +S+WS W PCS +CGVG R V+ NGG C + L S C C
Sbjct: 7 LLIRLIVVIWLTISMVTACPRAPTHLNHGRRTRG----DNGYKLIVADGP----NGYVPGKTYNLLLLGSRTHLKVQHFTHFTITAEAHTGARRPQAASPRR-VGRFQLFSDSLTQFNDRCVNTVSEADDLPKTEVQVMWVAPESGSGCVSLSAMVYEGPRAWFADDGNLSTVICERKPDAAAAQ------------------KECCACDEAKYSFVFEGIWSNETHPKDYPFAIWLTHFSDVIGASHESNFSFWGENHIATAGFRSLAEWGSPAALETELRAN--GPKLRTLIKAAGLWYPN-VNQNTSSKFRVDRKHPKVSLVSMFGPSPDWVVGISGLDLCTEDCSWKESMDFDLFPWDAGTDSGISYMSPNSETQ---PPERMYRITTM--YPEDPRAPFYNPKSREMTP-----LAKLYLRREKIV-----SRNCDDEF----LQALQLEVSDDAEEQD-TRAE-----CRVGDYSAWSPCSVSCGKGIRMRSRQYLYPAAADQNKCARQLVAKEMCVAAIPECADGPAQSKDRDDDEGENLANSQSLVGSNGEGAGLCKTSPWSVWSECSASCGI-GITMRTRTFVNHLGRKRC-----PHITIVEKNKCMRPDCTYEQVELPDPQCPTSQWSDWSPCSSTCGRGVT-----------IRTRLL----LLENGPDKESCTQRM--------ELHQQKECVNPIDCHINAEQAKDICVQAPD--------PGPCRGTY---MRYAYDPQNQHCYSFTYGGCRGNRNNFLTENDCLNTCNVLRSPYSSRVDQPRACVLSDWSVWSPCSVSCGVGVSESRRYVVTEPQNGGQPCSKRLVKSRSCAMPAC 763
BLAST of Spondin-1 vs. Ensembl Fly
Match: CG17739 (gene:FBgn0033710 transcript:FBtr0087893) HSP 1 Score: 195.282 bits (495), Expect = 2.120e-51 Identity = 199/836 (23.80%), Postives = 322/836 (38.52%), Query Frame = 2
Query: 356 FEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPD-EGLGHF-IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNP-INCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKP----------KHNWECTKMNAKQV---------KEFDEMNSNKNGNSQ-----FDGSSLTKKRRHINK----------------------KSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLP---LLESRTCE-----------------------------GRKRSC------------DFSAP-CSLLSWSRWGPCNATCEMHGYQQRSR------MFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVI--ICSEGLIMGSSCGKPVMKY-FYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRK----------DKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDG----NSNNFRSYHSCIKACMPDQIDFMKTAPIKACQVSEWSHWGPCSTTCG 2512
+ PG+ Y + L+ A G+ F F + +L +ES D LG + I + +CPN + + T V+ W+AP +P CI A V++ + + G L K +C ++ + D +V CCAC A Y++TF G WS+ THPKD+P ++++IGA+HT DY + G AS+ ++ + +G + L+ + +++ H+++ G+ + ++ + F V+ HH S ++M+ PSPDW GV+ +++C+ +C+W ++ +L+P+DAG +G +Y + Q PD +R I + FP P ++KP + C +++QV +DE + Q F +L + R N P P+ + C S WSEW+ C+VTCG G RSR L + C+ L E++ CE KRS D+ P C + WS + PC C HG +QR R + ++ R K+ EE C + CD ++ C + L V + +YD C + C + N F SK CE+TC LP+ ++ D + N+ + KYGG C + R RC Y CD + F ++A P+ P C+ SEWS W PC+ TCG
Sbjct: 52 YVPGQRYNVSLS-AFSGLSFISFML--------ALDLESGDGDGDANALGTWEIADLAETRFSPRCPNLVENTNTNVKTRVDVFWVAPSSPGQGCILLRATVMQHRDVWFMDDGFLTKRMCEEEVDDI--------------DTQPSIVDPCCACDEAKYELTFEGKWSRHTHPKDFPANSWRTRFSDIIGASHTLDYRFWQYGELASEGLREVAEHGSTRTLESELKDQS--EHIRTIIKARGIAYPNVTGKTFAVF-RVDSNHHLISLVSMVDPSPDWIVGVSGLELCLPNCSWVENKVHNLYPWDAGTDSGPSYMSADQP-QVPPDVVRRIK----SNFPNDPRSPFYDPTGAQMKPLATLHINRRRLYEKNCESSDSEQVPPECATNSWSRWDECTTKCGPGKQYRIREFKNPALASRHRCNNALREEKNCVGHKCAGFNEETAEGGEPEVAPSPGSSDDPQCGLSDWSEWSSCTVTCGTGEMTRSRHYLNKKAKKKCQKASRARLHETKICEAMECGGDIENEGGAGEPEEEQAGGDGGGSAEKRSIFRNFESYSQHREDYIPPVCGVTPWSDFSPCMGPCGGHGKRQRIRKVWNNNQVYGVTDPNDDGQDPCRHIKLHEEVNCTNPS--CDTIVPGFCYDELTDSPCRDNDVANFWYYDHVSDQCAIYWSDRCDTNRNKFKSKEECEETC-----RLPRHKQELQHDGIPAIDCLVSDWVSHSCNASCGDGFQLRTRRVLRTPKYGGKPCPKHL---------VRLDRC----YQRCDDMYSISGRGFGERRHVVRAPQPE--------PQDECRYSEWSAWTPCTATCG 828
BLAST of Spondin-1 vs. Ensembl Fly
Match: CG30046 (gene:FBgn0050046 transcript:FBtr0087895) HSP 1 Score: 176.407 bits (446), Expect = 3.357e-45 Identity = 156/633 (24.64%), Postives = 262/633 (41.39%), Query Frame = 2
Query: 365 GESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF----IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNP-INCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDI-PDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCR----HLPLLESRTCEGR--------------------------KRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKK-----------DTALCDPVIICSEGLIMGSSCG---KPVMKYF--YDAAKHSCV-PFEYLGCKGSLNNF 2104
G+ Y + LN A G + F + +L+ E+ ++ LG F +I +F C N + + T++ TW+AP P C+ A V + + + G L K IC + S+ + P CCAC A Y++ F G+WS+ HPKD+P + + L+GA H+ DY + +G AS A++ + S +L+++F+ +++ G + I + V+ HH SF + + PSPDW GV +++C+ +CTW ++ ++L+P+D G +G TY +SD+ + PD IR + + FP P + +KP + K+ + ++ ++++ N +DP + C T PWS W+ CS CG+G + R R + EL R + E R C+G + S A C L SW W PC+ TC GY+ R R + + + C R ++ E ++C T + P S G I G+ G +P + F YD +++ V FE KG++ N+
Sbjct: 60 GQEYNVSLN-AFNGHRYISFIM--------ALENENGDFSYNDDLGRFELSDLIETRFSP---NCINMVENTNTNSKTHMHLTWVAPSEPGSGCVLIRATVQQHREVWHMDDGGLTKRICEEVTDDVESQPTAPAAVDVP----------CCACDEARYELIFEGVWSRNLHPKDFPTRGWETRFCELLGAAHSSDYRFWESGELASVAMKEYAEHCSSRLLEREFSINFRDQKIRTIIKARGP-SYPNISSKTMASVRVDPIHHMVSFASKIEPSPDWIVGVTGLELCLRNCTWMEEKVINLYPWDVGTDSGPTY--TSSDQPQVPPDVIRRMR----SDFPNDPRSPFYDISGTPMKP-----MAILTVKRQRIYERRCADEDSN---------------------NDPDVPRE----CFTHPWSSWSDCSSKCGVGMQYRRRVY---KQPELARVYNCNEAQYEERECQGEMCGQNNLMREPEEFEDLEPDPRRIGYQPSQQRRAECELSSWGSWSPCSVTC-GDGYEMRQRQYLNPQAEFDC--QGVHRMELQETRKCSGRDCLGSLPGSYSTDMDSPYGGASPGRIGGNMYGPQLEPEAENFGDYDDSRNGAVGGFEVGNLKGNIGNW 627
BLAST of Spondin-1 vs. Ensembl Fly
Match: CG30046 (gene:FBgn0050046 transcript:FBtr0339627) HSP 1 Score: 176.407 bits (446), Expect = 3.357e-45 Identity = 156/633 (24.64%), Postives = 262/633 (41.39%), Query Frame = 2
Query: 365 GESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF----IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNP-INCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDI-PDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCR----HLPLLESRTCEGR--------------------------KRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKK-----------DTALCDPVIICSEGLIMGSSCG---KPVMKYF--YDAAKHSCV-PFEYLGCKGSLNNF 2104
G+ Y + LN A G + F + +L+ E+ ++ LG F +I +F C N + + T++ TW+AP P C+ A V + + + G L K IC + S+ + P CCAC A Y++ F G+WS+ HPKD+P + + L+GA H+ DY + +G AS A++ + S +L+++F+ +++ G + I + V+ HH SF + + PSPDW GV +++C+ +CTW ++ ++L+P+D G +G TY +SD+ + PD IR + + FP P + +KP + K+ + ++ ++++ N +DP + C T PWS W+ CS CG+G + R R + EL R + E R C+G + S A C L SW W PC+ TC GY+ R R + + + C R ++ E ++C T + P S G I G+ G +P + F YD +++ V FE KG++ N+
Sbjct: 60 GQEYNVSLN-AFNGHRYISFIM--------ALENENGDFSYNDDLGRFELSDLIETRFSP---NCINMVENTNTNSKTHMHLTWVAPSEPGSGCVLIRATVQQHREVWHMDDGGLTKRICEEVTDDVESQPTAPAAVDVP----------CCACDEARYELIFEGVWSRNLHPKDFPTRGWETRFCELLGAAHSSDYRFWESGELASVAMKEYAEHCSSRLLEREFSINFRDQKIRTIIKARGP-SYPNISSKTMASVRVDPIHHMVSFASKIEPSPDWIVGVTGLELCLRNCTWMEEKVINLYPWDVGTDSGPTY--TSSDQPQVPPDVIRRMR----SDFPNDPRSPFYDISGTPMKP-----MAILTVKRQRIYERRCADEDSN---------------------NDPDVPRE----CFTHPWSSWSDCSSKCGVGMQYRRRVY---KQPELARVYNCNEAQYEERECQGEMCGQNNLMREPEEFEDLEPDPRRIGYQPSQQRRAECELSSWGSWSPCSVTC-GDGYEMRQRQYLNPQAEFDC--QGVHRMELQETRKCSGRDCLGSLPGSYSTDMDSPYGGASPGRIGGNMYGPQLEPEAENFGDYDDSRNGAVGGFEVGNLKGNIGNW 627
BLAST of Spondin-1 vs. Ensembl Fly
Match: CG30203 (gene:FBgn0050203 transcript:FBtr0273303) HSP 1 Score: 174.096 bits (440), Expect = 2.126e-44 Identity = 144/607 (23.72%), Postives = 246/607 (40.53%), Query Frame = 2
Query: 203 LCLHWMIQESFAMNTRTNCFKMHKKTYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMED-SLQMESHKMIPDEGLGHFIINKKFGLVDYK----CPNRMHGNDNMFLTYVESTWIAPHNP-INCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLK-NAKTELCRHLPLLESRTCEGRK--------------------RSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRC 1942
+ H ++ + R C ++ + D F+I+I + + P Y + L+ + M F F++ E ED S M GHF + G+ D + C N + + Y+ +WIAP NP C+ +A VV+ + + G L K ICP ++ ++ S +TP ++ CCAC A Y++ W++ TH KD+P + +IGA+H+ Y + GG+AS+ ++ + +G + L+ + E T +++ G+ + + ++ HH S + PSPDW GVA +++C+++CTW + ++L+P+D G +G +Y + + Q PD +R I ++Y P P +KP T+ ++E +E + ++ PL+ C PW+EWT CS CG G R R N L E R C+G + S A C L +W W PC TC G R+R + + +++CL R + E + C
Sbjct: 1 MLRHIVLLILLSSEVRGVCNRIPQGASGPRSPVDDNFKILI----DGNPETYVPEHQYNVSLSCPI-NMKFVSFTLVV--EAEDPSAAFGGQDMT-----GHFEL---LGVGDTRFSTSCENMVENTNTNAKIYIAVSWIAPRNPDSGCVLIKAGVVQHRDVWFLDDGFLTKRICPEEIDELNS--------------LTPPLETCCACDEAKYEIVLERKWARNTHWKDFPSEDWRTRLGEVIGASHSHSYRYWAYGGRASKGMKELAEHGATRTLENEIRENTQNGEVRTIIKAPGIPYRPKTFGTTLANARLDPVHHQISLAAKIDPSPDWILGVAGLELCLSNCTWLERKVLNLYPWDIGTDSGPSYM-SSDEPQVPPDVVRRITSSY----PSDHRSPFYDDTGAPMKPLATLYLTRKKL-YIRECEE----------------------VPQEGPLE-----------CAVHPWNEWTNCSTRCGQGYSHRQRSFKNPNLAANFNCDRRLEEFRQCQGTQCGAAEEELEGEEPGLGMENPPSSGSMAECQLTNWGEWSPCTKTC-GRGSSTRTRDYYNPQARQRCL--SVMRIPLEETREC 536
BLAST of Spondin-1 vs. Ensembl Zebrafish
Match: spon1b (spondin 1b [Source:ZFIN;Acc:ZDB-GENE-000427-10]) HSP 1 Score: 224.172 bits (570), Expect = 2.735e-61 Identity = 165/572 (28.85%), Postives = 264/572 (46.15%), Query Frame = 2
Query: 275 KTYQKVMMKDG--EFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF-IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMW---NEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKC-SVTCGIGTEMRSRDMLKNAKTELCRHLPLLESR---TCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARF-----------EEKEKCLKNKE 1909
+T + +DG EFR+ + + ++PG +YR+ L + FR F++ A E + + PD+ G F +I++ CP + + T ++ W AP + C+ +A VV+ + ++ G L K +C E++ KP +ECCACG A Y+VTF G WS+K HPKD+P++ + HW+ LIGA+HT Y ++ GG AS+ V+ + YG ++++ ++ + + T W I + S SV+R H SFLTM+GPSPDW G++ D+C DC W DL P+DAG +G +Y E+ ++ IP + IR + + Q PF P+ RV+ ++I K +C + D++ ++ + +G + + CI S WS W+ C S TC G MR R ML K +L +LP L ++ C G S + ++ C + W W PC+A+C M G + R R +F EE EKC+ N +
Sbjct: 44 RTQARSARRDGQNEFRLKVEGE----PETYQPGSTYRVSLYASSPSY-FRGFTLIALKEGREGVS-------PDDYAGTFKLIDESDTQFMTNCPPAVTESIPRRRTRIQVFWTAPPSGSGCVTLKASVVQRRIIFFQDEGSLTKRLC---------EKDPVAITEKP-------AQECCACGTAKYRVTFYGNWSEKLHPKDYPRRAN--HWSALIGASHTRSYVLWEYGGFASEGVKQVAEYGSPVKMEEEIRQK--GDDVMTVIKTKAQWPAWQPLNIRAAPSAEFSVDRVRHLMSFLTMLGPSPDWNVGLSGEDLCTRDCGWVQKLVKDLVPWDAGTDSGVSY--ESPNKPSIPQERIRPLTSLDHPQSPFYDPSGGPMTPLGRVVVERIARKGE-QCNTVP----DTIDDIVADIAQEEKEEGDNTPE----------------------TCIYSNWSPWSACSSSTCDKGRRMRQR-ML---KAQLDPNLPCLHTQDFEPCMGPGCSEEEASTCMMSEWISWSPCSASCGM-GMRSRERYVKQFPEDGSSCTLPTEETEKCVVNDD 549 HSP 2 Score: 51.6026 bits (122), Expect = 4.366e-6 Identity = 32/90 (35.56%), Postives = 43/90 (47.78%), Query Frame = 2
Query: 1598 TFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
+C+ SPWS+W +CSV+CG+G R R + K C L + C + D C L WS W CN +C G+ RSRM
Sbjct: 608 AVVCMLSPWSDWGECSVSCGVGMRTRQRMLKTPVKPGQCPD-ELEQVEKCMLPECPTD----CMLSEWSAWSECNKSC-GKGHMIRSRMV 691
BLAST of Spondin-1 vs. Ensembl Zebrafish
Match: spon1b (spondin 1b [Source:ZFIN;Acc:ZDB-GENE-000427-10]) HSP 1 Score: 223.787 bits (569), Expect = 4.120e-61 Identity = 165/572 (28.85%), Postives = 264/572 (46.15%), Query Frame = 2
Query: 275 KTYQKVMMKDG--EFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF-IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMW---NEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKC-SVTCGIGTEMRSRDMLKNAKTELCRHLPLLESR---TCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARF-----------EEKEKCLKNKE 1909
+T + +DG EFR+ + + ++PG +YR+ L + FR F++ A E + + PD+ G F +I++ CP + + T ++ W AP + C+ +A VV+ + ++ G L K +C E++ KP +ECCACG A Y+VTF G WS+K HPKD+P++ + HW+ LIGA+HT Y ++ GG AS+ V+ + YG ++++ ++ + + T W I + S SV+R H SFLTM+GPSPDW G++ D+C DC W DL P+DAG +G +Y E+ ++ IP + IR + + Q PF P+ RV+ ++I K +C + D++ ++ + +G + + CI S WS W+ C S TC G MR R ML K +L +LP L ++ C G S + ++ C + W W PC+A+C M G + R R +F EE EKC+ N +
Sbjct: 44 RTQARSARRDGQNEFRLKVEGE----PETYQPGSTYRVSLYASSPSY-FRGFTLIALKEGREGVS-------PDDYAGTFKLIDESDTQFMTNCPPAVTESIPRRRTRIQVFWTAPPSGSGCVTLKASVVQRRIIFFQDEGSLTKRLC---------EKDPVAITEKP-------AQECCACGTAKYRVTFYGNWSEKLHPKDYPRRAN--HWSALIGASHTRSYVLWEYGGFASEGVKQVSEYGSPVKMEEEIRQKGD--DVMTVIKTKAQWPAWQPLNIRAAPSAEFSVDRVRHLMSFLTMLGPSPDWNVGLSGEDLCTRDCGWVQKLVKDLVPWDAGTDSGVSY--ESPNKPSIPQERIRPLTSLDHPQSPFYDPSGGPMTPLGRVVVERIARKGE-QCNTVP----DTIDDIVADIAQEEKEEGDNTPE----------------------TCIYSNWSPWSACSSSTCDKGRRMRQR-ML---KAQLDPNLPCLHTQDFEPCMGPGCSEEEASTCMMSEWISWSPCSASCGM-GMRSRERYVKQFPEDGSSCTLPTEETEKCVVNDD 549 HSP 2 Score: 51.2174 bits (121), Expect = 6.130e-6 Identity = 32/90 (35.56%), Postives = 43/90 (47.78%), Query Frame = 2
Query: 1598 TFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
+C+ SPWS+W +CSV+CG+G R R + K C L + C + D C L WS W CN +C G+ RSRM
Sbjct: 608 AVVCMLSPWSDWGECSVSCGVGMRTRQRMLKTPVKPGQCPDE-LEQVEKCMLPECPTD----CMLSEWSAWSECNKSC-GKGHMIRSRMV 691
BLAST of Spondin-1 vs. Ensembl Zebrafish
Match: spon1b (spondin 1b [Source:ZFIN;Acc:ZDB-GENE-000427-10]) HSP 1 Score: 223.402 bits (568), Expect = 4.653e-61 Identity = 165/572 (28.85%), Postives = 264/572 (46.15%), Query Frame = 2
Query: 275 KTYQKVMMKDG--EFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF-IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMW---NEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKC-SVTCGIGTEMRSRDMLKNAKTELCRHLPLLESR---TCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARF-----------EEKEKCLKNKE 1909
+T + +DG EFR+ + + ++PG +YR+ L + FR F++ A E + + PD+ G F +I++ CP + + T ++ W AP + C+ +A VV+ + ++ G L K +C E++ KP +ECCACG A Y+VTF G WS+K HPKD+P++ + HW+ LIGA+HT Y ++ GG AS+ V+ + YG ++++ ++ + + T W I + S SV+R H SFLTM+GPSPDW G++ D+C DC W DL P+DAG +G +Y E+ ++ IP + IR + + Q PF P+ RV+ ++I K +C + D++ ++ + +G + + CI S WS W+ C S TC G MR R ML K +L +LP L ++ C G S + ++ C + W W PC+A+C M G + R R +F EE EKC+ N +
Sbjct: 44 RTQARSARRDGQNEFRLKVEGE----PETYQPGSTYRVSLYASSPSY-FRGFTLIALKEGREGVS-------PDDYAGTFKLIDESDTQFMTNCPPAVTESIPRRRTRIQVFWTAPPSGSGCVTLKASVVQRRIIFFQDEGSLTKRLC---------EKDPVAITEKP-------AQECCACGTAKYRVTFYGNWSEKLHPKDYPRRAN--HWSALIGASHTRSYVLWEYGGFASEGVKQVSEYGSPVKMEEEIRQKGD--DVMTVIKTKAQWPAWQPLNIRAAPSAEFSVDRVRHLMSFLTMLGPSPDWNVGLSGEDLCTRDCGWVQKLVKDLVPWDAGTDSGVSY--ESPNKPSIPQERIRPLTSLDHPQSPFYDPSGGPMTPLGRVVVERIARKGE-QCNTVP----DTIDDIVADIAQEEKEEGDNTPE----------------------TCIYSNWSPWSACSSSTCDKGRRMRQR-ML---KAQLDPNLPCLHTQDFEPCMGPGCSEEEASTCMMSEWISWSPCSASCGM-GMRSRERYVKQFPEDGSSCTLPTEETEKCVVNDD 549 HSP 2 Score: 51.2174 bits (121), Expect = 6.232e-6 Identity = 32/90 (35.56%), Postives = 43/90 (47.78%), Query Frame = 2
Query: 1598 TFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
+C+ SPWS+W +CSV+CG+G R R + K C L + C + D C L WS W CN +C G+ RSRM
Sbjct: 608 AVVCMLSPWSDWGECSVSCGVGMRTRQRMLKTPVKPGQCPDE-LEQVEKCMLPECPTD----CMLSEWSAWSECNKSC-GKGHMIRSRMV 691
BLAST of Spondin-1 vs. Ensembl Zebrafish
Match: spon1b (spondin 1b [Source:ZFIN;Acc:ZDB-GENE-000427-10]) HSP 1 Score: 218.009 bits (554), Expect = 2.671e-59 Identity = 168/572 (29.37%), Postives = 266/572 (46.50%), Query Frame = 2
Query: 275 KTYQKVMMKDG--EFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF-IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMW---NEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKC-SVTCGIGTEMRSRDMLKNAKTELCRHLPLLESR---TCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARF-----------EEKEKCLKNKE 1909
+T + +DG EFR+ + + ++PG +YR+ L + FR F++ A E + + PD+ G F +I++ CP + + T ++ W AP + C+ +A VV+ + ++ G L K +C +E+ N + T VK CCACG A Y+VTF G WS+K HPKD+P++ + HW+ LIGA+HT Y ++ GG AS+ V+ + YG ++++ ++ + + T W I + S SV+R H SFLTM+GPSPDW G++ D+C DC W DL P+DAG +G +Y E+ ++ IP + IR + + Q PF P+ RV+ ++I K +C + D++ ++ I ++ +D T CI S WS W+ C S TC G MR R ML K +L +LP L ++ C G S + ++ C + W W PC+A+C M G + R R +F EE EKC+ N +
Sbjct: 29 RTQARSARRDGQNEFRLKVEGE----PETYQPGSTYRVSLYASSPSY-FRGFTLIALKEGREGVS-------PDDYAGTFKLIDESDTQFMTNCPPAVTESIPRRRTRIQVFWTAPPSGSGCVTLKASVVQRRIIFFQDEGSLTKRLC--------EKESLN-------EHCTNKVK-CCACGTAKYRVTFYGNWSEKLHPKDYPRRAN--HWSALIGASHTRSYVLWEYGGFASEGVKQVAEYGSPVKMEEEIRQK--GDDVMTVIKTKAQWPAWQPLNIRAAPSAEFSVDRVRHLMSFLTMLGPSPDWNVGLSGEDLCTRDCGWVQKLVKDLVPWDAGTDSGVSY--ESPNKPSIPQERIRPLTSLDHPQSPFYDPSGGPMTPLGRVVVERIARKGE-QCNTVP----DTIDDIVAD------------------IAQEEKEEDNTPET-----CIYSNWSPWSACSSSTCDKGRRMRQR-ML---KAQLDPNLPCLHTQDFEPCMGPGCSEEEASTCMMSEWISWSPCSASCGM-GMRSRERYVKQFPEDGSSCTLPTEETEKCVVNDD 533 HSP 2 Score: 51.6026 bits (122), Expect = 4.678e-6 Identity = 32/90 (35.56%), Postives = 43/90 (47.78%), Query Frame = 2
Query: 1598 TFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
+C+ SPWS+W +CSV+CG+G R R + K C L + C + D C L WS W CN +C G+ RSRM
Sbjct: 592 AVVCMLSPWSDWGECSVSCGVGMRTRQRMLKTPVKPGQCPD-ELEQVEKCMLPECPTD----CMLSEWSAWSECNKSC-GKGHMIRSRMV 675
BLAST of Spondin-1 vs. Ensembl Zebrafish
Match: spon1a (spondin 1a [Source:ZFIN;Acc:ZDB-GENE-000427-9]) HSP 1 Score: 216.083 bits (549), Expect = 1.834e-58 Identity = 166/589 (28.18%), Postives = 266/589 (45.16%), Query Frame = 2
Query: 308 EFRIVIRDMKGKMADRFEPGESYRIIL--NNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF-IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMT--PMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGM------WNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIK---------PKHNWECTKMNAKQVKEFDE-----MNSNKNGNSQFDGSSLTKKRR-----------------HINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF-----------ARFEEKEKCL 1897
EFR+ + + ++PG +YR+ L N Y FR F++ A E + Q E + G+F II+++ CP + + T ++ W AP + C+ +A +V+ + ++ G L K +C E +++ + E T P++ +CCACG A Y+VTF G WS+K HPKD+P++ + HW+ LIGA+H+ +Y ++ GG AS+ V+ + G + EE I+ M W + + S SV+RT H SFLTM+GPSPDW G+++ D+C +C W DL P+DAG +G +Y E+ ++ P + IR + + Q PF A PVAR++ ++I P + + A++ KE D+ + SN + S S+ K +R H P P ++ + C+ S W W+ CSV+CG G R R + + P E+ C + C S+ C + W W C+ATC + G ++R RM A E EKC+
Sbjct: 60 EFRLRVEGD----PEHYQPGSTYRVTLYATNPAY---FRGFTLIALKEGHNGDQEEDYA-------GNFQIIDEEDTQFMTSCPPAVTESTPRRRTRIQVFWTAPPSGHGCVLLKASIVQRKIISFQDEGSLTKRMC----------EKESL-----YGETTDKPLL-DCCACGTAKYRVTFYGNWSEKLHPKDYPRRAN--HWSALIGASHSKNYILWEYGGYASEGVKQVAELGSPVKM-----EEEIRQKGDEVLTVIKMKAQWPAWQPLNVRAAPSAEFSVDRTRHLMSFLTMLGPSPDWNVGLSSEDLCTRECGWVQKVVQDLIPWDAGTDSGVSY--ESPNKSTAPQEKIRPLTSLDHPQSPFYDPEGGAITPVARLVVERIARKGEQCNIVPDNVDDIVADIAQEEKEEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPHTQDFEPCMGPGCSDEDSSTCMMSEWITWSPCSVSCGSGLRSRERYVKQFPDDGFACTHPTEETEPCTVNEE-CSPSS-CLVTEWGEWDACSATCGL-GMKRRERMVKMPPSDGSICGAEVVEVEKCM 606 HSP 2 Score: 50.8322 bits (120), Expect = 6.977e-6 Identity = 36/93 (38.71%), Postives = 48/93 (51.61%), Query Frame = 2
Query: 1598 TFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSC---DFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
T C+ SPWS+W+ CSVTCG GT +R R MLK+A EL LE + C + C + WS W C+ +C G+ R+RM
Sbjct: 612 TIPCLLSPWSDWSDCSVTCGKGTRVRQR-MLKSA-VELGECNEDLE------QMEKCMLPECPMDCIMSEWSEWSECSKSC-GKGHLLRTRMI 695
BLAST of Spondin-1 vs. Ensembl Xenopus
Match: spon1 (spondin 1, extracellular matrix protein [Source:NCBI gene;Acc:100036727]) HSP 1 Score: 218.779 bits (556), Expect = 2.813e-59 Identity = 160/540 (29.63%), Postives = 253/540 (46.85%), Query Frame = 2
Query: 356 FEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF-IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAE--ETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKC-SVTCGIGTEMRSRDMLKNAKTELCRHLPLLES-RTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARF-----------EEKEKCLKNKE 1909
++PG +YR+ L A FR F++ A E ++ + E H G F II+ + CP + + T ++ W AP C+ +A +V+ + ++ G L K +C + + E N K + +CCACG A Y++TF G WS+KTHPKD+P++ + HW+ +IG +H+ +Y ++ GGQAS V+ + G ++++ + + + +K+ W + + S SV+R+ H SFL MMGPSPDW G+++ D+C +C W DL P+DAG +G TY E+ ++ IP D IR + + Q PF +PVARV+ ++I ++ D+ N + +T+++ DD CI S WS W+ C S TC G MR R MLK A+ +L P + C G S D ++ C + W W PC+A+C M G + R R +F EE EKC+ N+E
Sbjct: 68 YKPGNTYRVTLLAASPAY-FRGFTLIALKEGKEGEKEEDHA-------GTFQIIDDEDTQFMSNCPVAVTESTPRRRTRIQVLWTAPPIGSGCVILKASIVQKKIIYFQDEGSLTKRMCEPDL----TLEGGNEKP----------IPDCCACGTAKYRLTFYGNWSEKTHPKDYPRRAN--HWSAIIGGSHSKEYVLWEYGGQASDGVKQVAELGSPVKMEEEIRQKGDEVLTVIKAKAQWPA-WQPLNVRAAPSAEFSVDRSRHLMSFLAMMGPSPDWNVGLSSEDLCTKECGWVQKVIQDLIPWDAGTDSGVTY--ESPNKPSIPQDKIRPLTSLDHPQSPFYDPEGGPIIPVARVVIERI---------------ARKGDQCNIVPDNVDDIVPDLVTEEKEE-------DD------TPETCIYSNWSPWSACSSATCDKGKRMRQR-MLK-AQLDLSVPCPDTQDFEPCMGPGCSDDEASTCMMSEWITWSPCSASCGM-GMRSRERYVKQFPEDGSMCKVPTEETEKCIVNEE 549 HSP 2 Score: 60.4622 bits (145), Expect = 1.186e-8 Identity = 53/166 (31.93%), Postives = 67/166 (40.36%), Query Frame = 2
Query: 1535 KKRRHINKKSPLD------DPT------LANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFA-------------------RFEEKEKCLKNKERRFKIFEEKR 1939
KKR + K +P D D T + T C+ SPWSEW+ CSVTCG GT R R + A+ C L + C C S C L WS W CN +C G+ R+RM R + +K N+ RR K EKR
Sbjct: 575 KKRHRMIKMTPADGSMCKADTTEVEKCMMPECHTIPCVLSPWSEWSDCSVTCGKGTRTRQRMLKSPAELGDCNE-ELKQVEKC--MLPECPIS--CELTEWSYWSECNKSC-GKGHMIRTRMITMEPQFGGAVCPETVQRKKCRLRKCQKSSGNERRRLKEAREKR 734
BLAST of Spondin-1 vs. Ensembl Xenopus
Match: spon1 (spondin 1, extracellular matrix protein [Source:NCBI gene;Acc:100036727]) HSP 1 Score: 218.009 bits (554), Expect = 4.329e-59 Identity = 158/540 (29.26%), Postives = 250/540 (46.30%), Query Frame = 2
Query: 356 FEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF-IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAE--ETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKC-SVTCGIGTEMRSRDMLKNAKTELCRHLPLLES-RTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARF-----------EEKEKCLKNKE 1909
++PG +YR+ L A FR F++ A E ++ + E H G F II+ + CP + + T ++ W AP C+ +A +V+ + ++ G L K +C + K + +CCACG A Y++TF G WS+KTHPKD+P++ + HW+ +IG +H+ +Y ++ GGQAS V+ + G ++++ + + + +K+ W + + S SV+R+ H SFL MMGPSPDW G+++ D+C +C W DL P+DAG +G TY E+ ++ IP D IR + + Q PF +PVARV+ ++I ++ D+ N + +T+++ DD CI S WS W+ C S TC G MR R MLK A+ +L P + C G S D ++ C + W W PC+A+C M G + R R +F EE EKC+ N+E
Sbjct: 64 YKPGNTYRVTLLAASPAY-FRGFTLIALKEGKEGEKEEDHA-------GTFQIIDDEDTQFMSNCPVAVTESTPRRRTRIQVLWTAPPIGSGCVILKASIVQKKIIYFQDEGSLTKRMCEPDRLGLTGGNEKP-------------IPDCCACGTAKYRLTFYGNWSEKTHPKDYPRRAN--HWSAIIGGSHSKEYVLWEYGGQASDGVKQVAELGSPVKMEEEIRQKGDEVLTVIKAKAQWPA-WQPLNVRAAPSAEFSVDRSRHLMSFLAMMGPSPDWNVGLSSEDLCTKECGWVQKVIQDLIPWDAGTDSGVTY--ESPNKPSIPQDKIRPLTSLDHPQSPFYDPEGGPIIPVARVVIERI---------------ARKGDQCNIVPDNVDDIVPDLVTEEKEE-------DD------TPETCIYSNWSPWSACSSATCDKGKRMRQR-MLK-AQLDLSVPCPDTQDFEPCMGPGCSDDEASTCMMSEWITWSPCSASCGM-GMRSRERYVKQFPEDGSMCKVPTEETEKCIVNEE 546 HSP 2 Score: 60.4622 bits (145), Expect = 1.334e-8 Identity = 53/166 (31.93%), Postives = 67/166 (40.36%), Query Frame = 2
Query: 1535 KKRRHINKKSPLD------DPT------LANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFA-------------------RFEEKEKCLKNKERRFKIFEEKR 1939
KKR + K +P D D T + T C+ SPWSEW+ CSVTCG GT R R + A+ C L + C C S C L WS W CN +C G+ R+RM R + +K N+ RR K EKR
Sbjct: 572 KKRHRMIKMTPADGSMCKADTTEVEKCMMPECHTIPCVLSPWSEWSDCSVTCGKGTRTRQRMLKSPAELGDCNE-ELKQVEKC--MLPECPIS--CELTEWSYWSECNKSC-GKGHMIRTRMITMEPQFGGAVCPETVQRKKCRLRKCQKSSGNERRRLKEAREKR 731
BLAST of Spondin-1 vs. Ensembl Xenopus
Match: spon1 (spondin 1, extracellular matrix protein [Source:NCBI gene;Acc:100036727]) HSP 1 Score: 215.312 bits (547), Expect = 2.104e-58 Identity = 161/540 (29.81%), Postives = 252/540 (46.67%), Query Frame = 2
Query: 356 FEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF-IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAE--ETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKC-SVTCGIGTEMRSRDMLKNAKTELCRHLPLLES-RTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARF-----------EEKEKCLKNKE 1909
++PG +YR+ L A FR F++ A E ++ + E H G F II+ + CP + + T ++ W AP C+ +A +V+ + ++ G L K +C + + E N K + +CCACG A Y++TF G WS+KTHPKD+P++ + HW+ +IG +H+ +Y ++ GGQAS V+ + G ++++ + + + +K+ W + + S SV+R+ H SFL MMGPSPDW G+++ D+C +C W DL P+DAG +G TY E+ ++ IP D IR + + Q PF +PVARV+ ++I ++ D+ N + +T+++ DD CI S WS W+ C S TC G MR R MLK A+ +L P + C G S D S C + W W PC+A+C M G + R R +F EE EKC+ N+E
Sbjct: 68 YKPGNTYRVTLLAASPAY-FRGFTLIALKEGKEGEKEEDHA-------GTFQIIDDEDTQFMSNCPVAVTESTPRRRTRIQVLWTAPPIGSGCVILKASIVQKKIIYFQDEGSLTKRMCEPDL----TLEGGNEK----------PIPDCCACGTAKYRLTFYGNWSEKTHPKDYPRRAN--HWSAIIGGSHSKEYVLWEYGGQASDGVKQVAELGSPVKMEEEIRQKGDEVLTVIKAKAQWPA-WQPLNVRAAPSAEFSVDRSRHLMSFLAMMGPSPDWNVGLSSEDLCTKECGWVQKVIQDLIPWDAGTDSGVTY--ESPNKPSIPQDKIRPLTSLDHPQSPFYDPEGGPIIPVARVVIERI---------------ARKGDQCNIVPDNVDDIVPDLVTEEKEE-------DD------TPETCIYSNWSPWSACSSATCDKGKRMRQR-MLK-AQLDLSVPCPDTQDFEPCMGPGCSDDAST-CMMSEWITWSPCSASCGM-GMRSRERYVKQFPEDGSMCKVPTEETEKCIVNEE 548 HSP 2 Score: 60.077 bits (144), Expect = 1.408e-8 Identity = 45/133 (33.83%), Postives = 56/133 (42.11%), Query Frame = 2
Query: 1598 TFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFA-------------------RFEEKEKCLKNKERRFKIFEEKR 1939
T C+ SPWSEW+ CSVTCG GT R R + A+ C L + C C S C L WS W CN +C G+ R+RM R + +K N+ RR K EKR
Sbjct: 551 TIPCVLSPWSEWSDCSVTCGKGTRTRQRMLKSPAELGDCNE-ELKQVEKC--MLPECPIS--CELTEWSYWSECNKSC-GKGHMIRTRMITMEPQFGGAVCPETVQRKKCRLRKCQKSSGNERRRLKEAREKR 677
BLAST of Spondin-1 vs. Ensembl Xenopus
Match: spon1 (spondin 1, extracellular matrix protein [Source:NCBI gene;Acc:100036727]) HSP 1 Score: 216.083 bits (549), Expect = 2.580e-58 Identity = 162/540 (30.00%), Postives = 252/540 (46.67%), Query Frame = 2
Query: 356 FEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF-IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAE--ETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKC-SVTCGIGTEMRSRDMLKNAKTELCRHLPLLES-RTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARF-----------EEKEKCLKNKE 1909
++PG +YR+ L A FR F++ A E ++ + E H G F II+ + CP + + T ++ W AP C+ +A +V+ + ++ G L K +C EE+ KP + +CCACG A Y++TF G WS+KTHPKD+P++ + HW+ +IG +H+ +Y ++ GGQAS V+ + G ++++ + + + +K+ W + + S SV+R+ H SFL MMGPSPDW G+++ D+C +C W DL P+DAG +G TY E+ ++ IP D IR + + Q PF +PVARV+ ++I ++ D+ N + +T+++ DD CI S WS W+ C S TC G MR R MLK A+ +L P + C G S D S C + W W PC+A+C M G + R R +F EE EKC+ N+E
Sbjct: 68 YKPGNTYRVTLLAASPAY-FRGFTLIALKEGKEGEKEEDH-------AGTFQIIDDEDTQFMSNCPVAVTESTPRRRTRIQVLWTAPPIGSGCVILKASIVQKKIIYFQDEGSLTKRMCE-------PEESIYWGNEKP-------IPDCCACGTAKYRLTFYGNWSEKTHPKDYPRRAN--HWSAIIGGSHSKEYVLWEYGGQASDGVKQVAELGSPVKMEEEIRQKGDEVLTVIKAKAQWPA-WQPLNVRAAPSAEFSVDRSRHLMSFLAMMGPSPDWNVGLSSEDLCTKECGWVQKVIQDLIPWDAGTDSGVTY--ESPNKPSIPQDKIRPLTSLDHPQSPFYDPEGGPIIPVARVVIERI---------------ARKGDQCNIVPDNVDDIVPDLVTEEKEE-------DD------TPETCIYSNWSPWSACSSATCDKGKRMRQR-MLK-AQLDLSVPCPDTQDFEPCMGPGCSDDAST-CMMSEWITWSPCSASCGM-GMRSRERYVKQFPEDGSMCKVPTEETEKCIVNEE 548 HSP 2 Score: 60.4622 bits (145), Expect = 1.280e-8 Identity = 53/166 (31.93%), Postives = 67/166 (40.36%), Query Frame = 2
Query: 1535 KKRRHINKKSPLD------DPT------LANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFA-------------------RFEEKEKCLKNKERRFKIFEEKR 1939
KKR + K +P D D T + T C+ SPWSEW+ CSVTCG GT R R + A+ C L + C C S C L WS W CN +C G+ R+RM R + +K N+ RR K EKR
Sbjct: 574 KKRHRMIKMTPADGSMCKADTTEVEKCMMPECHTIPCVLSPWSEWSDCSVTCGKGTRTRQRMLKSPAELGDCNE-ELKQVEKC--MLPECPIS--CELTEWSYWSECNKSC-GKGHMIRTRMITMEPQFGGAVCPETVQRKKCRLRKCQKSSGNERRRLKEAREKR 733
BLAST of Spondin-1 vs. Ensembl Xenopus
Match: spon1 (spondin 1, extracellular matrix protein [Source:NCBI gene;Acc:100036727]) HSP 1 Score: 215.698 bits (548), Expect = 3.514e-58 Identity = 161/540 (29.81%), Postives = 252/540 (46.67%), Query Frame = 2
Query: 356 FEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF-IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAE--ETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKC-SVTCGIGTEMRSRDMLKNAKTELCRHLPLLES-RTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARF-----------EEKEKCLKNKE 1909
++PG +YR+ L A FR F++ A E ++ + E H G F II+ + CP + + T ++ W AP C+ +A +V+ + ++ G L K +C + + E N K + +CCACG A Y++TF G WS+KTHPKD+P++ + HW+ +IG +H+ +Y ++ GGQAS V+ + G ++++ + + + +K+ W + + S SV+R+ H SFL MMGPSPDW G+++ D+C +C W DL P+DAG +G TY E+ ++ IP D IR + + Q PF +PVARV+ ++I ++ D+ N + +T+++ DD CI S WS W+ C S TC G MR R MLK A+ +L P + C G S D S C + W W PC+A+C M G + R R +F EE EKC+ N+E
Sbjct: 75 YKPGNTYRVTLLAASPAY-FRGFTLIALKEGKEGEKEEDHA-------GTFQIIDDEDTQFMSNCPVAVTESTPRRRTRIQVLWTAPPIGSGCVILKASIVQKKIIYFQDEGSLTKRMCEPDL----TLEGGNEK----------PIPDCCACGTAKYRLTFYGNWSEKTHPKDYPRRAN--HWSAIIGGSHSKEYVLWEYGGQASDGVKQVAELGSPVKMEEEIRQKGDEVLTVIKAKAQWPA-WQPLNVRAAPSAEFSVDRSRHLMSFLAMMGPSPDWNVGLSSEDLCTKECGWVQKVIQDLIPWDAGTDSGVTY--ESPNKPSIPQDKIRPLTSLDHPQSPFYDPEGGPIIPVARVVIERI---------------ARKGDQCNIVPDNVDDIVPDLVTEEKEE-------DD------TPETCIYSNWSPWSACSSATCDKGKRMRQR-MLK-AQLDLSVPCPDTQDFEPCMGPGCSDDAST-CMMSEWITWSPCSASCGM-GMRSRERYVKQFPEDGSMCKVPTEETEKCIVNEE 555 HSP 2 Score: 60.077 bits (144), Expect = 1.411e-8 Identity = 53/166 (31.93%), Postives = 67/166 (40.36%), Query Frame = 2
Query: 1535 KKRRHINKKSPLD------DPT------LANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFA-------------------RFEEKEKCLKNKERRFKIFEEKR 1939
KKR + K +P D D T + T C+ SPWSEW+ CSVTCG GT R R + A+ C L + C C S C L WS W CN +C G+ R+RM R + +K N+ RR K EKR
Sbjct: 581 KKRHRMIKMTPADGSMCKADTTEVEKCMMPECHTIPCVLSPWSEWSDCSVTCGKGTRTRQRMLKSPAELGDCNE-ELKQVEKC--MLPECPIS--CELTEWSYWSECNKSC-GKGHMIRTRMITMEPQFGGAVCPETVQRKKCRLRKCQKSSGNERRRLKEAREKR 740
BLAST of Spondin-1 vs. Ensembl Mouse
Match: Spon1 (spondin 1, (f-spondin) extracellular matrix protein [Source:MGI Symbol;Acc:MGI:2385287]) HSP 1 Score: 219.935 bits (559), Expect = 8.947e-60 Identity = 160/549 (29.14%), Postives = 260/549 (47.36%), Query Frame = 2
Query: 350 DRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHK---MIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPM-VKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEET--IKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIK---------PKHNWECTKMNAKQVKEFDE-----MNSNKNGNSQFDGSSLTKKRRHINK--KSPLD---------------DPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
D ++PG SYR+ L+ A FR F++ A E ++ + E H I DE F+ N CP + + T ++ WIAP C+ +A +V+ + ++ G L K +C E++ + D +T + +CCACG A Y++TF G WS+KTHPKD+P++ + HW+ +IG +H+ +Y ++ GG AS+ V+ + G ++++ +++ + +K+ W + + S SV+RT H SFLTMMGPSPDW G++A D+C +C W DL P+DAG +G TY E+ ++ IP + IR + + Q PF + VARV+ ++I P + + A + K+ D+ + SN + S S+ K +R + K+ LD P ++ C S W W+ CSV+CG+G R R + + + LP E+ C + C S+ C + W W C+ATC M G ++R RM
Sbjct: 70 DFYKPGSSYRVTLSAAPPSY-FRGFTLIALKENQEGDKEEDHAGTFQIIDEEETQFMSN---------CPVAVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLC---------EQDPTL------DGVTDRPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRAN--HWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPA-WQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTY--ESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEEKDEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSVSCGMGMRSRERYVKQFPEDGSVCMLPTEETEKCTVNEE-CSPSS-CLVTEWGEWDDCSATCGM-GMKKRHRMV 585 HSP 2 Score: 53.5286 bits (127), Expect = 1.416e-6 Identity = 40/123 (32.52%), Postives = 53/123 (43.09%), Query Frame = 2
Query: 1535 KKRRHINKKSPLDDPT------------LANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
KKR + K SP D + T C+ SPWSEW+ CSVTCG G R R + A+ C L ++ C + D C L WS+W CN +C G+ R+R
Sbjct: 579 KKRHRMVKMSPADGSMCKAETSQAEKCMMPECHTIPCLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNE-DLEQAEKCMLPECPID----CELSEWSQWSECNKSC-GKGHMIRTRTI 695
BLAST of Spondin-1 vs. Ensembl Mouse
Match: Spon1 (spondin 1, (f-spondin) extracellular matrix protein [Source:MGI Symbol;Acc:MGI:2385287]) HSP 1 Score: 117.087 bits (292), Expect = 1.858e-28 Identity = 72/242 (29.75%), Postives = 122/242 (50.41%), Query Frame = 2
Query: 350 DRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHK---MIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPM-VKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSY 1063
D ++PG SYR+ L+ A FR F++ A E ++ + E H I DE F+ N CP + + T ++ WIAP C+ +A +V+ + ++ G L K +C E++ + D +T + +CCACG A Y++TF G WS+KTHPKD+P++ + HW+ +IG +H+ +Y ++ GG AS+ V+ + G + EE I+ +++Y
Sbjct: 70 DFYKPGSSYRVTLSAAPPSY-FRGFTLIALKENQEGDKEEDHAGTFQIIDEEETQFMSN---------CPVAVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLC---------EQDPTL------DGVTDRPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRAN--HWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKM-----EEEIRQQVRAY 279
BLAST of Spondin-1 vs. Ensembl Mouse
Match: Spon2 (spondin 2, extracellular matrix protein [Source:MGI Symbol;Acc:MGI:1923724]) HSP 1 Score: 102.449 bits (254), Expect = 4.071e-23 Identity = 76/303 (25.08%), Postives = 128/303 (42.24%), Query Frame = 2
Query: 800 CCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPEN--SDRQDI------PDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCG-IGTEMRSR 1681
C A +A Y +TF+G WS+ PK +P + W++L+GA H+ DY ++ S ++ G++ L ++ E L+S S + V+ H SF+ + PSPDW G+ ++D+C WK+ +DL+P DAG +GFT+ N + QD P N+ Y + + P+A+V + +++ + + ++ GN D S+ ++PLD C S WS W C CG +G + R+R
Sbjct: 34 CTARPLARYSITFIGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNEYVSNGLRDFAERGEAWALMKEI--EAAGEKLQSVHAVFSAPAIPSGTGQTSTELEVHPRHSLVSFVVRIVPSPDWFVGIDSLDLCEGG-RWKEQVVLDLYPHDAGTDSGFTFSSPNFATIPQDTVTEITASSPSHPANSFYYPRL--KSLPPIAKVTFVRLQ----------QSPRAFAPPSLDLASRGNEIVDSLSV--------PETPLD-----------CEVSLWSSWGLCGGPCGKLGAKSRTR 302
BLAST of Spondin-1 vs. Ensembl Mouse
Match: Spon2 (spondin 2, extracellular matrix protein [Source:MGI Symbol;Acc:MGI:1923724]) HSP 1 Score: 72.7886 bits (177), Expect = 3.404e-14 Identity = 40/147 (27.21%), Postives = 68/147 (46.26%), Query Frame = 2
Query: 800 CCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKD 1240
C A +A Y +TF+G WS+ PK +P + W++L+GA H+ DY ++ S ++ G++ L ++ E L+S S + V+ H SF+ + PSPDW G+ ++D+C WK+
Sbjct: 34 CTARPLARYSITFIGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNEYVSNGLRDFAERGEAWALMKEI--EAAGEKLQSVHAVFSAPAIPSGTGQTSTELEVHPRHSLVSFVVRIVPSPDWFVGIDSLDLCEGG-RWKE 177
BLAST of Spondin-1 vs. Ensembl Mouse
Match: Tfpi (tissue factor pathway inhibitor [Source:MGI Symbol;Acc:MGI:1095418]) HSP 1 Score: 64.3142 bits (155), Expect = 8.310e-11 Identity = 48/165 (29.09%), Postives = 75/165 (45.45%), Query Frame = 2
Query: 2009 CGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMKTAPIKACQVSEWSHWGPCST 2503
C + YF++ H C F Y GC+G+ N F + C+KTC+ P + K + ++ S +++ + C LE G C G M RY Y+N+ +C +F Y GC GN NNF + C K C + +P+ Q+S++ G T
Sbjct: 59 CKAMIRSYFFNMYTHQCEEFIYGGCEGNENRFDTLEECKKTCI------PGYEKTAV-----KAASGAER-------PDFCFLEEDPGL--CRGYMK---RYLYNNQTKQCERFVYGGCLGNRNNFETLDECKKIC---ENPVHSPSPVNEVQMSDYVTDGNTVT 197
BLAST of Spondin-1 vs. UniProt/SwissProt
Match: sp|Q9HCB6|SPON1_HUMAN (Spondin-1 OS=Homo sapiens OX=9606 GN=SPON1 PE=1 SV=2) HSP 1 Score: 220.32 bits (560), Expect = 4.656e-59 Identity = 160/549 (29.14%), Postives = 259/549 (47.18%), Query Frame = 2
Query: 350 DRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHK---MIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPM-VKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEET--IKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIK---------PKHNWECTKMNAKQVKEFDE-----MNSNKNGNSQFDGSSLTKKRRHINK--KSPLD---------------DPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
D ++PG SYR+ L+ A FR F++ A E + + E H I DE F+ N CP + + T ++ WIAP C+ +A +V+ + ++ G L K +C E++ FD +T + +CCACG A Y++TF G WS+KTHPKD+P++ + HW+ +IG +H+ +Y ++ GG AS+ V+ + G ++++ +++ + +K+ W + + S SV+RT H SFLTMMGPSPDW G++A D+C +C W DL P+DAG +G TY E+ ++ IP + IR + + Q PF + VARV+ ++I P + + A + K+ D+ + SN + S S+ K +R + K+ LD P ++ C S W W+ CS++CG+G R R + + + LP E+ C + C S+ C + W W C+ATC M G ++R RM
Sbjct: 70 DFYKPGTSYRVTLSAAPPSY-FRGFTLIALRENREGDKEEDHAGTFQIIDEEETQFMSN---------CPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLC---------EQDST------FDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRAN--HWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPA-WQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTY--ESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEEKDEDDTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSISCGMGMRSRERYVKQFPEDGSVCTLPTEETEKCTVNE-ECSPSS-CLMTEWGEWDECSATCGM-GMKKRHRMI 585 HSP 2 Score: 53.9138 bits (128), Expect = 6.578e-6 Identity = 34/90 (37.78%), Postives = 44/90 (48.89%), Query Frame = 2
Query: 1598 TFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
T C+ SPWSEW+ CSVTCG G R R + A+ C L + C + D C L WS+W CN +C G+ R+RM
Sbjct: 612 TIPCLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNE-DLEQVEKCMLPECPID----CELTEWSQWSECNKSC-GKGHVIRTRMI 695
BLAST of Spondin-1 vs. UniProt/SwissProt
Match: sp|Q9GLX9|SPON1_BOVIN (Spondin-1 OS=Bos taurus OX=9913 GN=SPON1 PE=1 SV=1) HSP 1 Score: 219.935 bits (559), Expect = 5.689e-59 Identity = 161/549 (29.33%), Postives = 258/549 (46.99%), Query Frame = 2
Query: 350 DRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHK---MIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPM-VKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEET--IKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIK---------PKHNWECTKMNAKQVKEFDE-----MNSNKNGNSQFDGSSLTKKRRHINK--KSPLD---------------DPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
D ++PG SYR+ L+ A FR F++ A E + + E H I DE F+ N CP + + T ++ WIAP C+ +A +V+ + ++ G L K +C E++ FD +T + +CCACG A Y++TF G WS+KTHPKD+P++ + HW+ +IG +H+ +Y ++ GG AS+ V+ + G ++++ +++ + +K+ W + + S SV+RT H SFLTMMGPSPDW G++A D+C +C W DL P+DAG +G TY E+ ++ IP + IR + + Q PF + VARV+ ++I P + + A + K+ D+ + SN + S S+ K +R + K+ LD P ++ C S W W+ CS++CG GT R R + + + LP E+ C + C S+ C W W C+ATC M G ++R RM
Sbjct: 70 DFYKPGTSYRVTLSAAPPSY-FRGFTLIALKENREGDKEEDHAGTFQIIDEEETQFMSN---------CPVAVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLC---------EQDST------FDGVTDKPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRAN--HWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPA-WQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTY--ESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEEKDEDDTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSISCGTGTRSRERYVKQFPEDGSVCTLPTEETEKCTVNEE-CSPSS-CLTTEWGEWDECSATCGM-GMKKRHRMV 585
BLAST of Spondin-1 vs. UniProt/SwissProt
Match: sp|Q8VCC9|SPON1_MOUSE (Spondin-1 OS=Mus musculus OX=10090 GN=Spon1 PE=1 SV=1) HSP 1 Score: 219.935 bits (559), Expect = 6.259e-59 Identity = 160/549 (29.14%), Postives = 260/549 (47.36%), Query Frame = 2
Query: 350 DRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHK---MIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPM-VKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEET--IKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIK---------PKHNWECTKMNAKQVKEFDE-----MNSNKNGNSQFDGSSLTKKRRHINK--KSPLD---------------DPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
D ++PG SYR+ L+ A FR F++ A E ++ + E H I DE F+ N CP + + T ++ WIAP C+ +A +V+ + ++ G L K +C E++ + D +T + +CCACG A Y++TF G WS+KTHPKD+P++ + HW+ +IG +H+ +Y ++ GG AS+ V+ + G ++++ +++ + +K+ W + + S SV+RT H SFLTMMGPSPDW G++A D+C +C W DL P+DAG +G TY E+ ++ IP + IR + + Q PF + VARV+ ++I P + + A + K+ D+ + SN + S S+ K +R + K+ LD P ++ C S W W+ CSV+CG+G R R + + + LP E+ C + C S+ C + W W C+ATC M G ++R RM
Sbjct: 70 DFYKPGSSYRVTLSAAPPSY-FRGFTLIALKENQEGDKEEDHAGTFQIIDEEETQFMSN---------CPVAVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLC---------EQDPTL------DGVTDRPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRAN--HWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPA-WQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTY--ESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEEKDEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSVSCGMGMRSRERYVKQFPEDGSVCMLPTEETEKCTVNEE-CSPSS-CLVTEWGEWDDCSATCGM-GMKKRHRMV 585 HSP 2 Score: 53.5286 bits (127), Expect = 9.904e-6 Identity = 40/123 (32.52%), Postives = 53/123 (43.09%), Query Frame = 2
Query: 1535 KKRRHINKKSPLDDPT------------LANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
KKR + K SP D + T C+ SPWSEW+ CSVTCG G R R + A+ C L ++ C + D C L WS+W CN +C G+ R+R
Sbjct: 579 KKRHRMVKMSPADGSMCKAETSQAEKCMMPECHTIPCLLSPWSEWSDCSVTCGKGMRTRQRMLKSLAELGDCNE-DLEQAEKCMLPECPID----CELSEWSQWSECNKSC-GKGHMIRTRTI 695
BLAST of Spondin-1 vs. UniProt/SwissProt
Match: sp|P35446|SPON1_RAT (Spondin-1 OS=Rattus norvegicus OX=10116 GN=Spon1 PE=1 SV=1) HSP 1 Score: 219.164 bits (557), Expect = 1.109e-58 Identity = 160/549 (29.14%), Postives = 259/549 (47.18%), Query Frame = 2
Query: 350 DRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHK---MIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPM-VKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEET--IKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIK---------PKHNWECTKMNAKQVKEFDE-----MNSNKNGNSQFDGSSLTKKRRHINK--KSPLD---------------DPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
D ++PG SYR+ L+ A FR F++ A E + + E H I DE F+ N CP + + T ++ WIAP C+ +A +V+ + ++ G L K +C E++ + D +T + +CCACG A Y++TF G WS+KTHPKD+P++ + HW+ +IG +H+ +Y ++ GG AS+ V+ + G ++++ +++ + +K+ W + + S SV+RT H SFLTMMGPSPDW G++A D+C +C W DL P+DAG +G TY E+ ++ IP + IR + + Q PF + VARV+ ++I P + + A + K+ D+ + SN + S S+ K +R + K+ LD P ++ C S W W+ CSV+CG+G R R + + + LP E+ C + C S+ C + W W C+ATC M G ++R RM
Sbjct: 70 DFYKPGSSYRVTLSAAPPSY-FRGFTLIALKENREGDKEEDHAGTFQIIDEEETQFMSN---------CPVAVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLC---------EQDPTL------DGVTDRPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRAN--HWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPS-WQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTY--ESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEEKDEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSVSCGMGMRSRERYVKQFPEDGSVCMLPTEETEKCTVNEE-CSPSS-CLVTEWGEWDDCSATCGM-GMKKRHRMV 585
BLAST of Spondin-1 vs. UniProt/SwissProt
Match: sp|Q9W770|SPON1_CHICK (Spondin-1 OS=Gallus gallus OX=9031 GN=SPON1 PE=2 SV=1) HSP 1 Score: 213.386 bits (542), Expect = 9.389e-57 Identity = 157/542 (28.97%), Postives = 248/542 (45.76%), Query Frame = 2
Query: 356 FEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHK---MIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEET--IKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKC-SVTCGIGTEMRSRDMLKNAKTELCRHLPLLES-RTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARF-----------EEKEKCLKNKE 1909
++PG SYR+ L+ A FR F++ A E ++ + E H I DE F+ N CP + + T ++ W AP C+ +A +V+ + ++ G L K IC + KP +CCACG A Y++TF G WS+KTHPKD+P++ + HW+ +IG++H+ +Y ++ GG AS+ V+ + G ++++ +++ + +K+ W + + S SV+R H SFLTM+GPSPDW G++A D+C DC W DL P+DAG +G TY E+ ++ +P + IR + + Q PF + VARV+ ++I + K F ++ + + +D T CI S WS W+ C S TC G MR R MLK A+ +L P + + C G S + + C + W W PC+ +C M G + R R +F EE EKC+ N+E
Sbjct: 67 YKPGNSYRVTLSAATPAY-FRGFTLIALKEGKEGDKEEDHAGTFQIIDEEETQFMSN---------CPVAVTESTPRRRTRIQVFWTAPPTGTGCVILKASIVQKRIIYFQDEGSLTKRICE-------QDSASEGVTDKP-------TLDCCACGTAKYRLTFYGNWSEKTHPKDFPRRTN--HWSAIIGSSHSKNYILWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPA-WQPLNVRAAPSAEFSVDRHRHLMSFLTMLGPSPDWNVGLSAEDLCTKDCGWVQKVVQDLIPWDAGTDSGVTY--ESPNKPTVPQEKIRPLTSLDHPQSPFYDPEGGSIKLVARVVLERI-----------------------ARKGEQCNFVPDNIDDIVADLAPEEKEEDDTPET-----CIYSNWSPWSACSSSTCEKGKRMRQR-MLK-AQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCMMSDWITWSPCSVSCGM-GTRSRERYVKQFPEDGSMCKVPTEETEKCIVNEE 548 HSP 2 Score: 56.9954 bits (136), Expect = 6.848e-7 Identity = 36/90 (40.00%), Postives = 47/90 (52.22%), Query Frame = 2
Query: 1598 TFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
T C+ SPWSEW+ CSVTCG G R R MLK+A + L ++ C + D C L WS+W CN +C G+ R+RM
Sbjct: 607 TIPCLLSPWSEWSDCSVTCGKGMRTRQR-MLKSAAELGDCNEELEQAEKCMLPECPID----CELTEWSQWSECNTSC-GKGHMIRTRMI 690
BLAST of Spondin-1 vs. TrEMBL
Match: A0A1Z1R177 (F-spondin (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 611.683 bits (1576), Expect = 0.000e+0 Identity = 291/295 (98.64%), Postives = 293/295 (99.32%), Query Frame = 2
Query: 1733 ESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMKTAPIKACQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC 2617
ESRTCEGRKRSCDFSA CSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEK CLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGN+NNFRSYHSCIKACMPDQIDFMKTAPIKACQVS+WSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC
Sbjct: 1 ESRTCEGRKRSCDFSALCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKACLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNNNNFRSYHSCIKACMPDQIDFMKTAPIKACQVSQWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC 295
BLAST of Spondin-1 vs. TrEMBL
Match: A0A5J4P3I4 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0011300 PE=4 SV=1) HSP 1 Score: 567.385 bits (1461), Expect = 0.000e+0 Identity = 341/937 (36.39%), Postives = 471/937 (50.27%), Query Frame = 2
Query: 299 KDGEFRIVIRDMK-GKMADR-FEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHFIINKKFGLVDYKCP------NRMHGNDNM-------------------FLTYVESTWIAPH-----------NP-INCIRFEAQVVKFDF--MVYESIGRLVKVICP--------------TKMYKMISEENK-------NMKISKP----FDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWP-KQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQ-------------------QFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFD---------------EMNSNKNGNS---QFDGSSLTKKRRH---------INKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATC-EMHGYQQRSRMFARFEEKEKC----LKNKERRFK-IFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQ---SNSDKRKKMPTM--ANKCRLEVKYGGMNCDGTMGPDI-RYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMP-----------------------------------DQI---DFMKT----APIKACQVSEWSHWGPCSTTCG--VGERFKWRSVLRPALNGGPACPELFLSEPC 2602
+D F + +R G M +EPG Y + L N + FRD ++ +DS ++ + GH NK+ Y + + GN ++T + +W AP NP + CI ++ V VY + G L K +CP T+ +++ + +MK+S D M PM +CCACG A+Y +TF GLW+++THP+DWP ++HWTNLIGA+H + IY G AS V ++C+YGD+TVLKQ Q I P L S +T GMW+E+ + +SRS + VNRTH SFL+M+GPSP+WC GVA+ +C DCTW +DLFP+DAG++NG TY P+ +DR+DIPDPIR I +M PFT N+PVARV +++ P+ W+CT + V+ FD E ++ NS G + K R H + SPL DP+LA MATFLCIT WS+W++CSVTCG+G R R ML N K ELC+H+PL+E CEGRKR+CDFS PCSLL W+ W PCNATC + +G Q R R R EE+++C + KE++ + +++C CDPV IC EG G +CG V ++Y A HSC+PFEY GCKG N F +K CEKTCL V +LP WR+++MA LQ+Q+ S+ R+KM M A C ++ G C+ + I +Y+ R RC +F Y GC GN N + Y SCI C+P DQ+ D + P + CQV+ W W PCS T +G R +WR ++RPA +GG C LF E C
Sbjct: 75 EDNGFELRVRRASDGVMVSGWYEPGREYVVTLTNRYQWVGFRDAILWLEVGRQDSHMKADPSLMKESNAGHIWTNKQGSQCQYHPSYLGAWIHHLTGNSATQSRFGCHSLTAERLNQARSWVTQLVLSWRAPSTFQTPVSGCPANPQLGCITIKSAVRARGGHNSVYAATGGLRKTLCPSVDRPPSPVLPAYMTEPEQLVGPSSTVSKRATGSMKLSSKDTMHMDNMQPM--DCCACGSATYNLTFQGLWTRETHPRDWPVHNPGLLHWTNLIGASHMPGFRIYQFGEPASAGVSAVCAYGDTTVLKQSLGIAASNTGSISSVPTGTQRGAMGIGP-LHSLISTPGMWSEETLTESRSTLVGVNRTHPLISFLSMLGPSPNWCAGVASQSVCQADCTWVKQLQIDLFPWDAGVRNGDTYIPKEADRKDIPDPIRYITADWMPNHPFTPNLPVARVTLERVLPREQWQCTSKLSDGVELFDSDGSTTVVGGRTAVKESERSQPVNSVVGDVTGGMMKKSRSHKSGSPSSGGLAGDSPLSDPSLAQMATFLCITDSWSQWSQCSVTCGVGRRERRRVMLVNKKNELCQHVPLVEEEPCEGRKRTCDFSLPCSLLPWTEWTPCNATCLDRNGQQTRRRYLVRPEERQQCEHLFVTTKEKQSNTVIAQRQCGPTDIECDPVTICGEGRKDGIACGNKVQSFYYSAVDHSCMPFEYSGCKGGRNRFATKQVCEKTCLGVVESLPAWRRERMALLQYQTSQIASDDTDREKMKYMKPAEHCG-QLMDPGEPCEYSRNQRIVAWYFSPRTRRCFEFVYLGCGGNKNRYADYKSCISDCLPQEWEKSQQMARALAATSKRSKYKADLDAHAPDSEVDQVTPTDLKRIDSLWGPKQDCQVTPWGGWSPCSVTRSYQIGMRMRWRHIVRPARHGGQPCGILFEQEHC 1007
BLAST of Spondin-1 vs. TrEMBL
Match: A0A183AG11 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS5896 PE=4 SV=1) HSP 1 Score: 559.296 bits (1440), Expect = 0.000e+0 Identity = 305/751 (40.61%), Postives = 396/751 (52.73%), Query Frame = 2
Query: 719 MYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWP-KQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQ----------------------QFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEF--------------DEMNSNKNGNSQFDGSSLTKK-----------------------RRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATC-EMHGYQQRSRMFARFEEKEKC-----LKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQ-SQSNSDK----RKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQ-----------------IDFMKTA----------------------------PIKACQVSEWSHWGPCSTTC--GVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC 2617
M+K+ + NM I +P D CCACG A+Y +TF GLW+++THP+DWP ++HWTNLIGA+H Y IY G AS V ++C+YGD+TVLKQ Q I P L S +T GMW+E + +SRS + VNRTH SFLTM+GPSP+WC GVA+ +C DCTW +DLFP+DAG+++G TY P+N+DR+D+PDPIR I +M PFT N PVARV +I PK +W+C K V+ F D S G +SL +++ +PL DP+LA MATFLCIT WS W++CSVTCG+G R R ML N K ELC+H+PL E CEGRKR+CDFS+PCSLL W+ W PCNATC +G Q R R AR E+ +C L ++ +FEE++C CDPV IC EG G CGK V Y Y A H+C+PF+YLGCKG N F +K CE+TCL AV +LP WR+++MA LQ+Q SQ SD+ RKK A C ++ G DG P +R+YY R RC +F Y GC GN N + +Y +CI CMP++ D+ A P + CQ++ W W PCS C G + +WR + RP +GG C LF C T C
Sbjct: 290 MHKISPKSTMNMDIMQPQD--------CCACGTATYNLTFQGLWTRETHPRDWPVHNPGLLHWTNLIGASHMPSYRIYQFGEPASAGVSAVCAYGDTTVLKQSLTMAAATAGSSGSVSSVPTGTQRGAIGIGP-LHSLISTPGMWSEATLNESRSTLVGVNRTHPLISFLTMLGPSPNWCAGVASQSVCQADCTWVKHLEIDLFPWDAGVRHGDTYVPKNADRKDVPDPIRYITADWMPNHPFTPNKPVARVTLDRILPKESWQCVSDVEKCVEMFRADGNTEAVGCTKRDIAKSQDAGTGGRLDNSLAADISSGTLPKKSRSKSGGGGGGSGGSGGLDQGNPLSDPSLAQMATFLCITDGWSAWSQCSVTCGVGKRERRRTMLLNKKNELCQHVPLREEEPCEGRKRTCDFSSPCSLLPWTEWTPCNATCANRNGLQTRRRYLARPAERTECVHLFKLPDERAMGMVFEERQCGPTDNECDPVTICGEGRKDGIPCGKKVRVYHYSAVDHACMPFDYLGCKGGRNRFATKELCERTCLKAVESLPGWRRERMALLQYQTSQLASDEENGGRKKDMKPAKHCTEQMDTGVSCDDGVTQPSVRWYYSPRTRRCFEFVYLGCGGNGNRYDNYTTCIADCMPEEWERSQQMAKALAATSKATDYRDGASGYDAQREDVAEDPLSASDVKQLSSLWGPRQDCQLTAWGGWSPCSAECRHQTGTQIRWRHIERPPRHGGTPCGMLFEKRSCRGTMC 1031
BLAST of Spondin-1 vs. TrEMBL
Match: A0A0R3U498 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS1462 PE=4 SV=2) HSP 1 Score: 557.755 bits (1436), Expect = 0.000e+0 Identity = 302/795 (37.99%), Postives = 429/795 (53.96%), Query Frame = 2
Query: 581 LTYVESTWIAPH-NPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKM--ISEENKN------MKISKPFD-------EMTP----MVKECCACGVASYKVTFMGLWSKKTHPKDWP-KQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQ-------------------QFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMN----SNKNGNSQFDGSSLTKKRRH-----------------------------INKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEM-HGYQQRSRMFARFEEKEKC-----LKNKERRFK--IFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQ----SQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDF--------------------------------MKTAPIKACQVSEWSHWGPCSTTCGV--GERFKWRSVLRPALNGGPACPELFLSEPCFA 2608
L +V + W P N NC+ A +VK V + +G L K +CP++M M + EN+ + S+ FD EM P K+CCACG A+YK+TF G+W+ THP+DWP K +++HWTNLIGA+H + IY G AS VQS+C+YGD+T+L++ Q A + P L+S T GMW E+ + + RS SVNRTH S LTM+GPSPDWCTG++ +C DCTW ++ T+ L+P+DAGI+ G TY P+ SDR++IPDPIR I+ ++M PF PVA V Q+++PK++W CT + V+ F + K + + D SSL +K R I +PL DP+LA MATFLCIT WS W+ CSVTCG+GT+ R+R ++ N K ELC+H+PL+ +CEGRKR+CD+SAPCSLL W+ W PC TC+ G++ R R AR EE +C +N+E R + + E + C+ + C+P IC EG G CG + +++Y A H C+ FEYLGCKG N F +K CEK C+ AV ALP WR+++M LQF+ + S+ D + C EV G CD +Y+ +R RCR F Y GC G N F ++ +C+ ACMP+++ + +P + C+V++W WGPCS C G++ + R++ RP+ GG AC L+ C+
Sbjct: 186 LPHVVAIWRTPSTNTSNCVTIHA-MVKSSTSVLKEVGDLTKTLCPSEMRPMEPVLSENRGSVDADAIDPSREFDRPAPDPVEMRPRDSMQPKDCCACGTATYKLTFQGVWNSTTHPQDWPYKHPNLLHWTNLIGASHAPSFQIYAIGQTASAGVQSVCAYGDTTILREALSLAASKAEGAAGVPTGSQSATTDVSP-LRSLITTPGMWGEETLMEERSTLFSVNRTHPLVSMLTMLGPSPDWCTGISGQSLCKADCTWVNNLTLHLYPWDAGIREGNTYMPKESDRREIPDPIRYIDESWMPGNPFRERKPVAVVKIQRVRPKYDWACTNTDEDGVQLFKMGGGIYVTGKKNDKESDESSLKEKAREQRCTTITAGKQKGKRTKSVGFGGPGASGTIGSNNPLSDPSLAQMATFLCITDEWSAWSACSVTCGVGTKTRTRRLIANKKPELCQHVPLVMEESCEGRKRTCDYSAPCSLLPWTLWSPCTGTCDSPRGHRSRKRALARPEEAAQCGHLWEGRNQEGREQGIMSEVEECQVPSETCEPATICGEGPKQGYPCGPKLRRFYYSAIDHECLEFEYLGCKGGRNMFETKQDCEKLCIPAVEALPAWRRERMGLLQFKTSQLTSSSPDDLEDSLKRDPVCYYEVNT-GYECDPPKELGNYWYFCSRTNRCRDFFYLGCGGTPNRFANHTACMAACMPNELRHSQLLAKVRSATASRKAAANATDADEEAPDGLEVGVSPRQDCRVTQWGAWGPCSVRCSSQRGQQIRLRAIERPSRYGGEACGPLYERRSCYG 977
BLAST of Spondin-1 vs. TrEMBL
Match: A0A5K3F8X7 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1) HSP 1 Score: 557.37 bits (1435), Expect = 1.026e-180 Identity = 300/792 (37.88%), Postives = 428/792 (54.04%), Query Frame = 2
Query: 581 LTYVESTWIAPH-NPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKM--ISEENKN------MKISKPFD-------EMTP----MVKECCACGVASYKVTFMGLWSKKTHPKDWP-KQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQ-------------------QFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEF---------DEMNSNKNGNSQFDGSSLTKK---------------------RRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEM-HGYQQRSRMFARFEEKEKC-----LKNKERRFK--IFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQ----SQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDF--------------------------------MKTAPIKACQVSEWSHWGPCSTTCGV--GERFKWRSVLRPALNGGPACPELFLSEPCFA 2608
L +V + W P N NC+ A +VK V + +G L K +CP++M M + EN+ + S+ FD EM P K+CCACG A+YK+TF G+W+ THP+DWP K +++HWTNLIGA+H + IY G AS VQS+C+YGD+T+L++ Q A + P L+S T GMW E+ + + RS SVNRTH S LTM+GPSPDWCTG++ +C DCTW ++ T+ L+P+DAGI+ G TY P+ SDR++IPDPIR I+ ++M PF PVA V Q+++PK++W CT + V+ F + N ++ S G S T++ I +PL DP+LA MATFLCIT WS W+ CSVTCG+GT+ R+R ++ N K ELC+H+PL+ +CEGRKR+CD+SAPCSLL W+ W PC TC+ G++ R R AR EE +C +N+E R + + E + C+ + C+P IC EG G CG + +++Y A H C+ FEYLGCKG N F +K CEK C+ AV ALP WR+++M LQF+ + S+ D + C EV G CD +Y+ +R RCR F Y GC G N F ++ +C+ ACMP+++ + +P + C+V++W WGPCS C G++ + R++ RP+ GG AC L+ C+
Sbjct: 186 LPHVVAIWRTPSTNTSNCVTIHA-MVKSSTSVLKEVGDLTKTLCPSEMRPMEPVLSENRGSVDADAIDPSREFDRPAPDPVEMRPRDSMQPKDCCACGTATYKLTFQGVWNSTTHPQDWPYKHPNLLHWTNLIGASHAPSFQIYAIGQTASAGVQSVCAYGDTTILREALSLAASKAEGAAGVPTGSQSATTDVSP-LRSLITTPGMWGEETLMEERSTLFSVNRTHPLVSMLTMLGPSPDWCTGISGQSLCKADCTWVNNLTLHLYPWDAGIREGNTYMPKESDRREIPDPIRYIDESWMPGNPFRERKPVAVVKIQRVRPKYDWACTNTDEDGVQLFKMGGGIYVTGKKNDKESDESSLKGGSATQQITAGKQKGKRTKSVGFGGPGASGTIGSNNPLSDPSLAQMATFLCITDEWSAWSACSVTCGVGTKTRTRRLIANKKPELCQHVPLVMEESCEGRKRTCDYSAPCSLLPWTLWSPCTGTCDSPRGHRSRKRALARPEEAAQCGHLWEGRNQEGREQGIMSEVEECQVPSETCEPATICGEGPKQGYPCGPKLRRFYYSAIDHECLEFEYLGCKGGRNMFETKQDCEKLCIPAVEALPAWRRERMGLLQFKTSQLTSSSPDDLEDSLKRDPVCYYEVNT-GYECDPPKELGNYWYFCSRTNRCRDFFYLGCGGTPNRFANHTACMAACMPNELRHSQLLAKVRSATASRKAAANATDADEEAPDGLEVGVSPRQDCRVTQWGAWGPCSVRCSSQRGQQIRLRAIERPSRYGGEACGPLYERRSCYG 974
BLAST of Spondin-1 vs. Ensembl Cavefish
Match: spon1b (spondin 1b [Source:ZFIN;Acc:ZDB-GENE-000427-10]) HSP 1 Score: 214.927 bits (546), Expect = 2.764e-58 Identity = 162/571 (28.37%), Postives = 269/571 (47.11%), Query Frame = 2
Query: 275 KTYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF-IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMT-PMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMW---NEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPFTAN-----VPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKC-SVTCGIGTEMRSRDMLKNAKTELCRHLPLLESR---TCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARF-----------EEKEKCLKNKE 1909
+T ++ +DG +R ++G D ++PG +YR+ L + FR F++ A E + + PD+ G+F II+++ CP + + T ++ W AP + C+ +A +V+ + ++ G L + +C E K++ F +T +++CCACG A Y+VTF G WS+K HPKD+P++ + HW+ LIGA+H+ +Y ++ GG AS+ V+ + G ++++ ++ + + T W I + S SV+R HH SFLTM+GPSPDW G++ ++C +C W DL P+DAG +G TY E+ ++ P + IR + + Q PF + P+ARV+ ++I ++ ++ N ++ I P +D T + CI S WS W+ C S TC G MR R ML K +L +P L ++ C G + + C L W W PC+A+C M G + R R +F EE EKC+ N++
Sbjct: 37 RTQRRGARRDGHNDYRLR-VEGD-PDTYQPGSTYRVSLYASSPSY-FRGFTLIALKEGREGVS-------PDDYAGNFKIIDEEETQFMTSCPPAVTESIPRRRTRIQVFWTAPPSGSGCVILKASIVQRRIIFFQDEGSLTRRLC---------EVQKSI-----FGGITLKPLQDCCACGTAKYRVTFYGNWSEKVHPKDYPRRAN--HWSALIGASHSRNYVLWEYGGYASEGVRQVAELGSPVKMEEEIRQK--GDDVMTVIKTKAQWPAWQPLNIRAAPSAEFSVDRAHHLMSFLTMLGPSPDWNVGISGENLCTQECGWVQRLVKDLLPWDAGTDSGVTY--ESPNKPTNPQERIRPLTSLDHPQSPFYSPHGGPITPLARVVVERI---------------ARKGEQCN------------TVPDTVDDIVADIPPED-TEEDNTPETCIYSNWSPWSACSSSTCDKGRRMRQR-ML---KAQLDATVPCLHTQDFEPCMGPGCREEEPSTCMLSEWISWSPCSASCGM-GMRSRERYIKQFPEDGSSCREPTEETEKCVVNED 544 HSP 2 Score: 58.151 bits (139), Expect = 3.237e-8 Identity = 37/87 (42.53%), Postives = 43/87 (49.43%), Query Frame = 2
Query: 1607 CITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
C+ SPWSEW CSVTCG+G +R R M A C PL E C + D C L WS W CN +C G+ RSRM
Sbjct: 606 CMVSPWSEWGHCSVTCGVGMRIRRRMMKTPALPNQCGD-PLEEVEKCMLPECPVD----CMLSDWSDWSECNKSCG-KGHMIRSRMV 686
BLAST of Spondin-1 vs. Ensembl Cavefish
Match: spon1a (spondin 1a [Source:ZFIN;Acc:ZDB-GENE-000427-9]) HSP 1 Score: 172.555 bits (436), Expect = 3.938e-44 Identity = 154/590 (26.10%), Postives = 248/590 (42.03%), Query Frame = 2
Query: 308 EFRIVIRDMKGKMADRFEPGESYRIIL--NNAMYGMDFRDFSIFAAYEMEDSLQMESHK---MIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMT--PMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGM------WNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPF-----TANVPVARVIYQKIK---------PKHNWECTKMNAKQVKEFDE-----MNSNKNGNSQFDGSSLTKKRR-----------------HINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF-----------ARFEEKEKCL 1897
EFR+ + + ++P +YR+ L N Y FR F++ A E + + E + I DE F+ N CP + + T ++ W AP + CI +A +V+ + ++ G L K +C E +++ + E T P++ +CCACG A Y+VTF G WS+K HPKD+P++ + HW+ +IG++H+ +Y ++ GG AS V+ + G + EE I+ M W + + S SV+R+ H SFLTM+GPSPDW G+ + + C W P+++ P+ IR + + Q PF A VARV+ ++I P + + A++ KE D+ + SN + S S+ K +R H P P ++ C+ S W W+ CSV+CG+G R R + + + LP E+ C + C S+ C + W W C+ATC + G ++R RM A E EKC+
Sbjct: 66 EFRLRVEGD----PEHYQPASTYRVTLYATNPAY---FRGFTLIALKEGRNGEKEEDYAGNFQIIDEEDTQFMTN---------CPPAVTESTPRRRTRIQVFWTAPPSGSGCILLKASIVQRKIISFQDEGSLTKRMC----------EKESL-----YGESTDKPLL-DCCACGTAKYRVTFYGNWSEKLHPKDYPRRAN--HWSAIIGSSHSKNYVLWEYGGYASDGVKQVAELGSPVKM-----EEEIRQKGDEVLTVIKMKAQWPAWQPLNVRAAPSAEFSVDRSRHLMSFLTMLGPSPDWNVGLLSSP--VGRCGW---------PWNSICSPNKPTVPQEK--------IRPLTSLDHPQSPFYDPEGGAITQVARVVVERIARKGEQCNIVPDNVDDIVADIAQEEKEEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPHTQDFEPCMGPGCSDEDATTCMMSEWITWSPCSVSCGLGMRSRERYVKQFPEDGFACTLPTEETEPCTVNE-DCSPSS-CLVTEWGEWDACSATCGL-GMKRRERMVKMPPSDGSICGAEVVEVEKCM 594 HSP 2 Score: 53.5286 bits (127), Expect = 9.828e-7 Identity = 31/78 (39.74%), Postives = 41/78 (52.56%), Query Frame = 2
Query: 1598 TFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATC 1831
T C+ SPWS+W+ CSVTCG GT MR R +LK+ R L + T S ++ C + WS W CN +C
Sbjct: 600 TIPCLLSPWSDWSDCSVTCGKGTRMRQR-ILKSP-----RGLSKCDQTTLISFCPSVSAASDCMMSDWSEWSECNKSC 671
BLAST of Spondin-1 vs. Ensembl Cavefish
Match: spon2b (spondin 2b, extracellular matrix protein [Source:ZFIN;Acc:ZDB-GENE-990415-161]) HSP 1 Score: 118.242 bits (295), Expect = 2.560e-28 Identity = 94/329 (28.57%), Postives = 152/329 (46.20%), Query Frame = 2
Query: 800 CCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDS-TVLKQ-QFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQ------FPFTANV-PVARVIYQKIKPKH--NWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCG-IGTEMRSRDMLKNAKTELCRHLPLLESRTC 1747
C A A Y +TF G WS+ PK +P + W+ LIG TH+ DY I+ AS V+ G++ T++K+ + A E I+ + F+T + D Q+ + F V H F SF+ + PSPDW GV ++++C D WK++ ++DLFP+DAG +GFT+ + + + IP D + I +++ + +P ++ P+A+V KIK + + Q+ +E+N + IN +PLD C S WS W C CG G R+R + + PL + + C
Sbjct: 51 CSAPSTAKYSLTFTGKWSQTAFPKQYPVYRPPAQWSPLIGVTHSSDYHIWQKNEYASNGVREFSEKGEAWTLIKEVEAAGERIQ-SVYGLFSTPAVL--DGTSQTTTEF-EVYARHSFLSFIMRLVPSPDWFVGVDSLNLCEGD-HWKENISLDLFPYDAGTDSGFTF--SSPNFETIPQDKVMQITSSFPSHPANSFYYPRLKHLPPIAKVTLTKIKSNQIFSLPIEPTQSNQIPTGNEINESL-----------------IN--TPLD-----------CEVSAWSPWGLCKGQCGEKGVRHRTRYIHIHPANNGAVCPPLEDEKPC 342
BLAST of Spondin-1 vs. Ensembl Cavefish
Match: spon2a (spondin-2-like [Source:NCBI gene;Acc:103039648]) HSP 1 Score: 104.76 bits (260), Expect = 5.575e-24 Identity = 72/236 (30.51%), Postives = 107/236 (45.34%), Query Frame = 2
Query: 761 SKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYF--------PENSDRQDIPDPIRTINTTY---MTQFPFTANVPVARVIYQKIKPKHN 1435
S F T V C A G ASY + F G WS +T PK +P + W+ L+ TH Y ++ G AS VQ+ G + L ++ E + S + T+G+ I S + I ++ + S L + PSPDW GV ++++C WK + T DL PFDAG +GFT+ PEN P N+ Y + Q P A + + R ++ +HN
Sbjct: 25 SSAFPLRTSNVTTCSARGPASYILVFTGHWSPQTFPKQYPLFRPPAQWSKLVAVTHNDQYRLWQEGSPASPGVQNFAELGVTVELVKEAKEARKTRAVGSMYRTAGI--PSGIGHSSTELI-LHPRNPLLSLLVKVIPSPDWFVGVDSLNLCEGG-QWKQEVTFDLHPFDAGTDSGFTFSSPNFPTMPPENITMITSQKPNHPANSFYYPRLPQLPPLATIWLRRQARSPVR-QHN 255
BLAST of Spondin-1 vs. Ensembl Cavefish
Match: ENSAMXT00000041207.1 (pep primary_assembly:Astyanax_mexicanus-2.0:11:5185373:5196079:-1 gene:ENSAMXG00000033686.1 transcript:ENSAMXT00000041207.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 73.559 bits (179), Expect = 2.111e-14 Identity = 51/170 (30.00%), Postives = 75/170 (44.12%), Query Frame = 2
Query: 1955 ALCDPVIICSEGLIM--GSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGT--MGP----DIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFM 2440
A C I + L+M G+ CG+ V +++YD A +C PF Y GC G+ N F ++ C TC+ K ++H + N M T N C E M T GP ++++YD + C +F Y GC GN N F S C C+ Q ++
Sbjct: 14 ATCFSHIPTTHLLVMDSGTPCGQYVQRWYYDKAVGACSPFWYGGCSGNSNRFKTEFECINTCILT-------SKSSVSHRHHSIRDNQCYYLDMTTGLNGCLNERNLFRMTLINTNDQGPCRAYLLKWFYDTQQNECARFWYGGCKGNGNRFDSQEECEDRCVTLQTPWV 176
BLAST of Spondin-1 vs. Ensembl Sea Lamprey
Match: spon2b (spondin 2b, extracellular matrix protein [Source:ZFIN;Acc:ZDB-GENE-990415-161]) HSP 1 Score: 87.0409 bits (214), Expect = 9.708e-19 Identity = 69/306 (22.55%), Postives = 133/306 (43.46%), Query Frame = 2
Query: 797 ECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDS-TVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQS---RSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHIN----KKSPLDDPTLANMATFLCITSPWSEWTKCSV-TCG-IGTEMRSR 1681
+C A G Y++ F G WS+ + PK +P + W++L+ H + ++ AS V+ + G++ ++++ A + +G+++ + S + S + + PSPDW GV ++++C + WK+ ++DL P+DAG +GFT+ + + IP +PI + ++ + + P Y K+K ++ M + + D + +T++ + K++PLD C + WS W CSV TCG G + R+R
Sbjct: 9 KCSAGGHPKYQLVFTGRWSQTSFPKQYPLFRPPAQWSSLVAVVHGRPFQMWRASRFASPGVREMSERGEAWGIMREVDAHAQARTAAARPPAVAGVFSAPALPNGTGQSSTELEFTAKQPLLSLMVRLVPSPDWFVGVDSLNLCEGE-RWKERLSLDLQPYDAGTDSGFTF--SSPNYATIPQEPITQMTSS-------SPSHPANSFYYPKLK--------QLPPLARLVLTRMGKRPHSSKHPDANLVTRQNEPASGVYIKQTPLD-----------CEVAKWSPWGLCSVDTCGQPGVKRRTR 285
BLAST of Spondin-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005038.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005038.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 64.3142 bits (155), Expect = 1.073e-11 Identity = 40/148 (27.03%), Postives = 69/148 (46.62%), Query Frame = 2
Query: 2009 CGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQS---NSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMK 2443
C + +YF++ SC F Y GC+G+LNNF SC + C+ L ++ K + +++ N + + + C ++ G C + R++Y+ + C F Y GC GN NNF S SC+ C Q+ +++
Sbjct: 84 CRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHCVHRAVPLKIKKRSKQTNNNNNNKAARCNGESNSEQFVLPRICLMKADPG--ECTAVI---PRFHYNPKTSNCESFTYGGCGGNDNNFSSERSCMSVC--GQVGYVQ 224 HSP 2 Score: 63.929 bits (154), Expect = 1.344e-11 Identity = 43/131 (32.82%), Postives = 60/131 (45.80%), Query Frame = 2
Query: 2027 KYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACM 2419
+ FY+ C PF Y GC G+ NNF S LA NA W D L +RK+ CRL+++ G C + RY+++ +G C +F Y GC GN NNF+ SC + C+
Sbjct: 18 RVFYNRYTQRCEPFHYGGCFGNGNNFES--------LAECNAKCAWIPDHRLQL---------RRKQTDIAPKACRLDMEVG--VCRALL---RRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHCV 126
BLAST of Spondin-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005043.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005043.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 63.929 bits (154), Expect = 1.193e-11 Identity = 40/148 (27.03%), Postives = 69/148 (46.62%), Query Frame = 2
Query: 2009 CGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQS---NSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMK 2443
C + +YF++ SC F Y GC+G+LNNF SC + C+ L ++ K + +++ N + + + C ++ G C + R++Y+ + C F Y GC GN NNF S SC+ C Q+ +++
Sbjct: 83 CRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHCVHRAVPLKIKKRSKQTNNNNNNKAARCNGESNSEQFVLPRICLMKADPG--ECTAVI---PRFHYNPKTSNCESFTYGGCGGNDNNFSSERSCMSVC--GQVGYVQ 223 HSP 2 Score: 58.9214 bits (141), Expect = 7.158e-10 Identity = 44/132 (33.33%), Postives = 60/132 (45.45%), Query Frame = 2
Query: 2027 KYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANK-CRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACM 2419
+ FY+ C PF Y GC G+ NNF S LA NA W D N P +A K CRL+++ G C + RY+++ +G C +F Y GC GN NNF+ SC + C+
Sbjct: 18 RVFYNRYTQRCEPFHYGGCFGNGNNFES--------LAECNAKCAWIPD-----------NKRLASPCPLVAPKACRLDMEVG--VCRALL---RRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHCV 125
BLAST of Spondin-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009465.1 (pep scaffold:Pmarinus_7.0:GL478507:22936:25713:-1 gene:ENSPMAG00000008558.1 transcript:ENSPMAT00000009465.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 63.929 bits (154), Expect = 2.519e-11 Identity = 42/148 (28.38%), Postives = 65/148 (43.92%), Query Frame = 2
Query: 2009 CGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQS-----NSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKAC-MPDQID 2434
C ++ YDA +C F Y GC G+ NNF + C TC ++ L R + + + S + +++ + + C LE C G+ R+ YD +G C++F Y GC GN NNF C+ C + D D
Sbjct: 81 CRGSFTRWIYDAESSNCKEFTYGGCGGNFNNFLEETECMVTCGRSLGTLRTCRYHRTLNYLVVNNSCFLISSCWMTRRLFHVQDVCLLEAVTS--PCRGSF---TRWTYDAESGHCKEFTYGGCGGNFNNFLEETECVAMCGLQDGFD 223 HSP 2 Score: 53.9138 bits (128), Expect = 4.507e-8 Identity = 45/137 (32.85%), Postives = 59/137 (43.07%), Query Frame = 2
Query: 2009 CGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKD-KMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKAC 2416
C ++ YDAA +C F Y GC+G+ NNF + C C R D KMA +Q S P + C LE G C G+ R+ YD + C++F Y GC GN NNF C+ C
Sbjct: 10 CRGSFTRWTYDAATSTCKEFTYGGCRGNQNNFLEEAECLMDC---------GRSDVKMAVYCWQFGS-------WPYI---CLLEEAVG--PCRGSF---TRWIYDAESSNCKEFTYGGCGGNFNNFLEETECMVTC 122
BLAST of Spondin-1 vs. Ensembl Sea Lamprey
Match: spon2a (spondin 2a, extracellular matrix protein [Source:ZFIN;Acc:ZDB-GENE-990415-160]) HSP 1 Score: 58.9214 bits (141), Expect = 3.394e-10 Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 2
Query: 2462 CQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC 2617
C+VS+W+ WG CS TCG+G + R V++ NGG CPEL + E C C
Sbjct: 134 CEVSDWAAWGFCSRTCGIGVKRSTRFVIQTPANGGRPCPELHMEESCVDRAC 185 HSP 2 Score: 51.9878 bits (123), Expect = 9.208e-8 Identity = 46/177 (25.99%), Postives = 70/177 (39.55%), Query Frame = 2
Query: 1151 SFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSR 1681
S + + PSPDW GV+++D+C N W + DL P+D G +GF + N + +PI I TA P P +++ ++ A F E + ++ L K I + A C S W+ W CS TCGIG + +R
Sbjct: 2 SIVVRLIPSPDWFVGVSSLDLCSNAGGWAPLVSHDLQPWDGGTDSGFAFSSPNYASEP-QEPISLI----------TAQRPS--------HPANSFYYPRLQALPRIGFLEFHLQPADHAFQRPDDLICKHCQIVRSDSGQREEAAG-TPLDCEVSDWAAWGFCSRTCGIGVKRSTR 158
BLAST of Spondin-1 vs. Ensembl Nematostella
Match: EDO49895 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RF43]) HSP 1 Score: 170.629 bits (431), Expect = 1.083e-46 Identity = 115/362 (31.77%), Postives = 176/362 (48.62%), Query Frame = 2
Query: 797 ECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNK---NGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFAR 1873
ECCACG A+YK+TF G WS++TH + P M W+ LIG +H+ Y ++ GG AS+AVQ + +G + L ++ + + ++ S T +W + +S S V+ T H S ++ M PSPDW GV V++C +CTWK + T+DL P+DAG +G T+ N+ R PI I TT + P + Y Q++ F + + +G D HI K+P + A C + WS+WT CSVTCG+G ++R RD+++ + + L E R C R+R C + C + WS W C +C + G + R+R R
Sbjct: 1 ECCACGKATYKLTFKGKWSQETHARHHPG--PMARWSPLIGCSHSNKYVMWRYGGIASRAVQMMSEWGATEYLDEEMRNQ--QENVMSIIQTPSLWYG--VGES-SAVFRVDSTRHLLSMISRMIPSPDWNVGVDRVNLCTTNCTWKHEETIDLIPWDAGTDDGITFKSPNA-RSTPKQPISRI-TTRLHDHPRAS-------FYLNTN------------DQIRPFASLKLERLAVSGGCSNDA--------HIAPKNPYPPKS----ARIDCQLTHWSKWTSCSVTCGVGEQIRGRDIIRVPEHDGIPCPNLYEHRPC-ARQR-CSSTKKCEVTPWSAWSSCRGSCGV-GRKVRTRRIIR 319 HSP 2 Score: 53.1434 bits (126), Expect = 2.759e-7 Identity = 22/52 (42.31%), Postives = 30/52 (57.69%), Query Frame = 2
Query: 2462 CQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC 2617
CQ++ WS W CS TCGVGE+ + R ++R + G CP L+ PC C
Sbjct: 234 CQLTHWSKWTSCSVTCGVGEQIRGRDIIRVPEHDGIPCPNLYEHRPCARQRC 285
BLAST of Spondin-1 vs. Ensembl Nematostella
Match: EDO47504 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLY4]) HSP 1 Score: 59.3066 bits (142), Expect = 1.582e-9 Identity = 34/87 (39.08%), Postives = 42/87 (48.28%), Query Frame = 2
Query: 2441 KTAPIKACQVSEWSHWGPCSTTCGVGERFKWRSVL-RPALNGGPACPELFLSEPCFATTC*KIYINFVSIFDDIYNNFMGKYILVLP 2698
K ++ C VS WS W PCS CGVG R + R V+ RP+ GG CP L CF C K +I IY +Y +LP
Sbjct: 86 KLQGVQDCVVSTWSRWSPCSHRCGVGMRKRSRRVIVRPSQRGGKECPPLRQKRGCFLEHCGK-ESGLANILPKIYKRLPNQYEEILP 171
BLAST of Spondin-1 vs. Ensembl Nematostella
Match: EDO48831 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIN8]) HSP 1 Score: 58.151 bits (139), Expect = 7.129e-9 Identity = 36/90 (40.00%), Postives = 44/90 (48.89%), Query Frame = 2
Query: 1622 WSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEG---RKRSCDFSAPCSLL--SWSRWGPCNATCEMHGYQQRSRMFARF 1876
W EW+ CSVTCG GT MR R+ C + L +C G SC + C + WSRWGPCNATC G + R+ F F
Sbjct: 12 WGEWSACSVTCGTGTRMRMRN---------CTNPAPLYGDSCHGDDKESESCSVTPSCKGIFSEWSRWGPCNATCG-PGVKMRTMNFTSF 91
BLAST of Spondin-1 vs. Ensembl Nematostella
Match: EDO38466 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCV3]) HSP 1 Score: 48.521 bits (114), Expect = 2.101e-7 Identity = 20/41 (48.78%), Postives = 27/41 (65.85%), Query Frame = 2
Query: 2294 MGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKAC 2416
M IR++YD R+ C+ F Y GC GN+NNF S +C+K C
Sbjct: 25 MARFIRWHYDMRSSECKMFTYGGCQGNANNFESKAACLKKC 65
BLAST of Spondin-1 vs. Ensembl Nematostella
Match: EDO26072 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TCY8]) HSP 1 Score: 48.1358 bits (113), Expect = 2.386e-7 Identity = 20/41 (48.78%), Postives = 27/41 (65.85%), Query Frame = 2
Query: 2294 MGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKAC 2416
M IR++YD R+ C+ F Y GC GN+NNF S +C+K C
Sbjct: 25 MARFIRWHYDMRSSECKMFTYGGCQGNANNFESKAACLKKC 65
BLAST of Spondin-1 vs. Ensembl Medaka
Match: spon1a (spondin 1 [Source:NCBI gene;Acc:101161963]) HSP 1 Score: 217.624 bits (553), Expect = 4.730e-59 Identity = 199/791 (25.16%), Postives = 325/791 (41.09%), Query Frame = 2
Query: 302 DGEFRIVIRDMKGKMADRFEPGESYRI--ILNNAMYGMDFRDFSIFAAYEMEDSLQMESHK---MIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGM------WNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIK---------PKHNWECTKMNAKQVKEFDE-----MNSNKNGNSQFDGSSLTKKRR-----------------HINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIIC--SEGLIMGSSCGKPVMKYFYDAAKHSCV--PFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMKTAPIKACQVSEWSHWGPCSTTCGVG 2518
+ EFR+ + D ++PG +YR+ I ++ Y FR F++ A E + + + I DE F+ N CP+ + + ++ W AP C+ +A +V+ + ++ G L K +C K S D + +CCACG A Y+VTF G WS+K HPKD+P++ + HW+ +IGA+H+ +Y ++ GG AS V+ + G + EE I+ M W + + S SV+R H SFLTMMGPSPDW G++A ++C +C W DL P+DAG +G +Y E+S++ +P + IR + + Q PF A PVARV+ ++I P + + A++ KE D+ + SN + S ++ K +R H P P ++ C+ S W W+ CS++CG G R R + + LP E+ C + C S+ C + W W C+ATC + G ++R RM + + + E + E ++C P ++ SE +CGK +H + P E C L M E A++ +W S+ N K +E ++GG C T+ + +C+ K + GNS++ + + Q+ T+ C++ WS W C+ CG G
Sbjct: 56 NNEFRLRVEGD----PDSYQPGSTYRVNVIASSPSY---FRGFTLIALKEGAGGDKDDDYAGNFRIIDEDETQFMTN---------CPHAVTESTPRRRNNIQVFWTAPPTGSGCVLLKASIVQKKIIFFQDEGPLTKQMC--------------EKESLYGDATDKPLLDCCACGTAKYRVTFYGNWSEKIHPKDYPRRAN--HWSAIIGASHSKNYVLWEYGGSASDGVKQVAELGSPVKM-----EEEIRQKGDDVLTVIKMKAQWPAWQPLNVRAAPSAEFSVDRIRHLMSFLTMMGPSPDWNVGLSAENLCTKECGWVQKVVQDLIPWDAGTDSGVSY--ESSNKPTVPQEKIRPLTSLDHPQSPFYDPEGGAITPVARVVVERIARKGEQCNIVPDNVDDIVADIAQEEKEEDDTPETCIYSNWSPWSACSSATCEKGKRMRQRMLKAQLDLSVPCPHTQDFEPCMGPGCSDEDGSTCMMSEWITWSPCSMSCGAGIRSRERYVKQFPDDGSICTLPTEETENCVVNEE-CSPSS-CLVTEWGEWDACSATCGL-GMKRRERMV-KMPPADGSICGAE----VLEVEKCMMPECHTIPCLLTPWSEWSDCSVTCGK------GSRTRHRVLKSPLELGECTEDLEQVEKCMLPECPIDCAMSDWTEW-----------SECNKSCGKGHTIRTRVVTMEPQFGGDPCPETI----------QRKKCKVRKCSRGQGNSDDKKRRKEQREKRRSKQVKTEVTSEHPGCKMRPWSSWTECTKQCGGG 772
BLAST of Spondin-1 vs. Ensembl Medaka
Match: spon1b (spondin-1 [Source:NCBI gene;Acc:101161803]) HSP 1 Score: 214.542 bits (545), Expect = 2.831e-58 Identity = 163/607 (26.85%), Postives = 270/607 (44.48%), Query Frame = 2
Query: 188 ILWMLLCLHWMIQESFAMNTRTNCFKMHKKTYQKVMMKDG--EFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHF-IINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMW---NEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPF-----TANVPVARVIYQKIKPKHNWECTKMN----------AKQVKEFDE-----MNSNKNGNSQFDGSSLTKKRR-----------------HINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTE---LCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
+L + C ++E + C ++ + Q+ K+G EFR+ + + + + PG +Y+++L A FR F++ A L+ + PD+ +G F I++K CP + T ++ W AP + C+ +A +V+ + ++ G L +C + ++ D + ECCACG A Y++TF G WS+K HPKD+P++ + HW+ LIGA+H+ +Y ++ GG AS+ V+ + +G ++ + ++ + + T W + + S SV+R H SFLTM+GPSPDW G++ D+C DC W DL P+DAG +G TY E+ ++ IP D IR + + Q PF P+ARV+ ++I K +C + ++ KE D+ + S + S ++ K RR H P P + C+ S W W+ CSV+CG+G MRSR+ E LC L E+ C + C + W W PC+ TC + G ++R RM
Sbjct: 11 VLSCVYCAVAFMEEPSGGRSDGFCERIPRAQIQRTR-KEGHHEFRLKVEGEQ----ENYNPGTTYKVLLL-ATSPAYFRGFTLIA-------LKEGTEGTTPDDYIGQFQIVDKDDTQFMTSCPPAVTETTPRRRTKIQVFWTAPPSGSGCVILKASIVQKRVIFFQDKGSLTVRLCEKEPVQL--------------DTTSAPPIECCACGTAKYRLTFYGNWSEKVHPKDYPRRAN--HWSALIGASHSRNYVLWEYGGYASEGVRQVAEFGSPIKMEGEIRQK--GDDVLTVIKTKAQWPAWQPLNLRAAPSAEFSVDRARHLMSFLTMLGPSPDWNVGLSGEDLCTKDCGWIQRLVADLIPWDAGTDSGVTY--ESPNKPTIPQDKIRPLTSFDHPQSPFYDPQGGPISPLARVVVERIARKGE-QCNVVPDNIDDIVADIGQEEKEEDDTPETCIYSAWSPWSACSSATCDKGRRMRQRMLKAQLDLSVPCPHTQDFQPCMAPGCSMEEPSTCMMSEWISWSACSVSCGMG--MRSRERYVKQYPEDGSLC-QLHTEETEKCVVNDEC--LPSSCVVTEWGEWDPCSVTCGL-GMRRRERMV 577 HSP 2 Score: 55.0694 bits (131), Expect = 2.916e-7 Identity = 32/87 (36.78%), Postives = 44/87 (50.57%), Query Frame = 2
Query: 1607 CITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMF 1867
C+ +PWSEW+KCS TCG G +R+R ++ CR E+ E R C C + WS W CN +C G+ R+RM
Sbjct: 607 CVPTPWSEWSKCSATCGRG--IRTRQRMRKTNQADCRE----ETEQKECRLPECSID--CMVSEWSEWSECNKSC-GKGHTIRTRMV 684
BLAST of Spondin-1 vs. Ensembl Medaka
Match: spon2b (spondin 2 [Source:NCBI gene;Acc:101164320]) HSP 1 Score: 112.464 bits (280), Expect = 1.926e-26 Identity = 89/334 (26.65%), Postives = 145/334 (43.41%), Query Frame = 2
Query: 761 SKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGF---ISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIP-DPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCG-IGTEMRSRDMLKNAKTELCRHLPLLESRTC 1747
S P PM C A A Y +TF G W++ PK +P + W+NLIG TH+ DY ++ AS V+ ++ L ++ ET ++S + G+ + + V H SF+ + PSPDW GV ++++C D WK+ T++LFP+DAG +GFT+ + + + IP D I I ++Y + P Y ++ KH K+ ++K+ +++ S +Q + + +PLD C S WS W C CG G + R+R ++ + L E R C
Sbjct: 30 SVPVPTDLPM---CTASEPAQYSLTFSGKWTRAAFPKQYPVYRPPAQWSNLIGVTHSLDYHMWQRNEFASNGVREFAEKSEAWTLMKEV--ETAGERIQSVY---GILSAPAVVGGTGQMDTEFEVFARHSLMSFMVRIIPSPDWFVGVDSINLCNGD-KWKESVTLELFPYDAGTDSGFTF--SSPNFETIPQDRITQITSSYPSH-------PANSFFYPRL--KHLPPIAKVKLTKIKKTNQIISLPMEPTQSNLLPTGNEIEETLINTPLD-----------CEVSVWSPWGLCKGKCGESGVQHRTRYVIMHPANNGVACPLLEEERKC 332
BLAST of Spondin-1 vs. Ensembl Medaka
Match: spon2a (spondin-2 [Source:NCBI gene;Acc:101163419]) HSP 1 Score: 96.2857 bits (238), Expect = 4.309e-21 Identity = 84/311 (27.01%), Postives = 130/311 (41.80%), Query Frame = 2
Query: 797 ECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTY----FPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMAT--------FLCITSPWSEWTKCSVTCGI-GTEMRSRDML 1690
EC A ASY + F G WS ++ PK +P + W+ LI +H + ++ G AS VQ G + L + E K + + T+G+ N I S S + + S + M PSPDW GV ++++C + WK + T++L P+DAG +GFT+ FP + P+ I I +Q P N P Y ++ KE + S K +T++ R N+++ L + L N + C S WS W C C G R+R +L
Sbjct: 37 ECTAKSPASYILVFTGHWSPQSFPKQYPLFRPPAQWSKLIAVSHNHHFRLWEEGAAASLGVQKFAELGLTVELMKAGKEARKKRTVGAMQRTAGIPN--GIGHS-STEVHMQPRSSLLSLMVKMIPSPDWFVGVNSLNLCEGN-HWKQEVTVNLQPYDAGTNSGFTFSSPKFPTSP-----PENISKIT----SQVP---NHPANSFYYPRL----------------KELPAIASIK----------ITRQHRSANRQTVLSNHILPNSISPRRFSETPLDCEVSLWSSWGLCLGPCSRGGIRHRTRYIL 305
BLAST of Spondin-1 vs. Ensembl Medaka
Match: COL6A3 (collagen type VI alpha 3 chain [Source:NCBI gene;Acc:101161016]) HSP 1 Score: 75.0998 bits (183), Expect = 3.482e-13 Identity = 47/155 (30.32%), Postives = 75/155 (48.39%), Query Frame = 2
Query: 1952 TALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKAC 2416
+ L DP CS G G C K++Y+ C F Y GC G+ N F S+ C K CL + L + ++ +Q ++Q +++ + P + C+L + G +C +++YYD + C +F Y GCDGN N F + C +AC
Sbjct: 2545 SELADP---CSLGFDPGMPCKDYQAKWYYERQNGFCTQFWYGGCGGNDNRFDSEALCLKRCLRSEVQLAG-KPEEKPQMQPEAQ-QTEQVEIPPAPVDICQLPKQEG--SCAEFA---LKWYYDTTSKSCTRFWYGGCDGNQNRFDTQEECKEAC 2689 HSP 2 Score: 51.2174 bits (121), Expect = 6.233e-6 Identity = 25/75 (33.33%), Postives = 37/75 (49.33%), Query Frame = 2
Query: 2213 DKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDF 2437
D + + +A+ C L GM C ++YY+ +NG C QF Y GC GN N F S C+K C+ ++
Sbjct: 2539 DNPQTVSELADPCSLGFD-PGMPCKDYQA---KWYYERQNGFCTQFWYGGCGGNDNRFDSEALCLKRCLRSEVQL 2609
BLAST of Spondin-1 vs. Planmine SMEST
Match: SMESG000009946.1 (SMESG000009946.1) HSP 1 Score: 1699.49 bits (4400), Expect = 0.000e+0 Identity = 807/807 (100.00%), Postives = 807/807 (100.00%), Query Frame = 2
Query: 197 MLLCLHWMIQESFAMNTRTNCFKMHKKTYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMKTAPIKACQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC 2617
MLLCLHWMIQESFAMNTRTNCFKMHKKTYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMKTAPIKACQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC
Sbjct: 1 MLLCLHWMIQESFAMNTRTNCFKMHKKTYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMKTAPIKACQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC 807
BLAST of Spondin-1 vs. Planmine SMEST
Match: SMESG000009946.1 (SMESG000009946.1) HSP 1 Score: 1684.08 bits (4360), Expect = 0.000e+0 Identity = 800/800 (100.00%), Postives = 800/800 (100.00%), Query Frame = 2
Query: 218 MIQESFAMNTRTNCFKMHKKTYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMKTAPIKACQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC 2617
MIQESFAMNTRTNCFKMHKKTYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMKTAPIKACQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC
Sbjct: 1 MIQESFAMNTRTNCFKMHKKTYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMKTAPIKACQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC 800
BLAST of Spondin-1 vs. Planmine SMEST
Match: SMESG000009946.1 (SMESG000009946.1) HSP 1 Score: 1651.34 bits (4275), Expect = 0.000e+0 Identity = 784/784 (100.00%), Postives = 784/784 (100.00%), Query Frame = 2
Query: 266 MHKKTYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMKTAPIKACQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC 2617
MHKKTYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMKTAPIKACQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC
Sbjct: 1 MHKKTYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFLTYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMISEENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWPKQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANMATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMKTAPIKACQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFATTC 784
BLAST of Spondin-1 vs. Planmine SMEST
Match: SMESG000039905.1 (SMESG000039905.1) HSP 1 Score: 263.848 bits (673), Expect = 2.637e-75 Identity = 223/781 (28.55%), Postives = 332/781 (42.51%), Query Frame = 2
Query: 236 AMNTRTNCFKMHKK---TYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFL----TYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMIS-EENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWP-KQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQF---AEETIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANM-ATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSC--GKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMP-TMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQIDFMKTAPI-------KACQVSEWSHWGPCSTTC 2509
A +TR N + H+K T + D + I + M ++ E Y ++ N EME+ + H + G + N CP R + +L +V WIAP P CIR + D + IC K KM + ++ S + T VK+C A Y++T W+ H KDWP K T +GA+H+ ++ IY+ GG A++ V +C G T L++QF + L + S N+ RS I VN T H SFL + PSPDWCTG++ D+C C W++ F + L+P+DAG+ +G TY +N + PI I +++ PFT V+ KIK + + E N N N Q+ S + + + P +N T C+ S WSEW+ CS +CG G + R R +LK K+E C L + +C + + CS W +W PCNATC G R R+F +EK +C++ E + + C + C P +IC E + G SC P +++YD + +C F Y GC G LNNF + C C + Q +R P T N+C + + + G+ CD + P R+YYDN N C +F++ GC GN+NNFR+ C C+ ++ T I K C S WS W C C
Sbjct: 35 AFSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSN--------------VHTEMEE---LSEHDW---KNAGEVVFNVTHASKTEYCPQRAYV---QYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDASSKTNK------RSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQT--PICPITSSWPKDNPFT-------VMNGKIK--------NIGVVTFQLIQEYNKNDN---QYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLSEWSEWSSCSESCGKGEKTRYRKLLK-GKSEQCIESSLTNTESCSSKCSTAISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASL----ECALASERCKPDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQ------------------RRFNTPETYPNRCGVSMTW-GIFCD-SQSPSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCLREKSTSTTTTTISQPVTVRKVCIYSNWSDWSSCDDNC 741
BLAST of Spondin-1 vs. Planmine SMEST
Match: SMESG000032462.1 (SMESG000032462.1) HSP 1 Score: 62.003 bits (149), Expect = 2.149e-9 Identity = 85/372 (22.85%), Postives = 132/372 (35.48%), Query Frame = 2
Query: 1607 CITSPWSEWTKCSVTCG--IGTEMRSRDMLKNAKTELC--RHLPLL-ESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEE--KEKCLKNKER--RFKIFEEKRCKKDTALCDPVIICSEGLIMGSSCGKPVMKYFYDAAKHSCVPFEYLGCKG-SLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMPTMANKCRLEVKYGGMNCDGTMGP----DIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYH-----------SCIKAC-MP-----------DQIDFMKTAPIKACQVSEWSHWGPCSTTCGVGERFKWRSVLRPALNGGPACPELFLSEPCFAT 2611
C PW W+ C CG GT RSR ++ KT C + P L E+ +C C + C WS W C+ +C G +R+R F + E E C+ E F+ K C + D S ++ CG ++ KH C G +N + C+ C + QW + +++ + N+ R C G+ P DIR Y + C +T SN+ S + +C +C MP D +D C VS WS W C C G +++ R++++ G +CP L + C +
Sbjct: 79 CEPDPWQSWSSCQGPCGNRTGTRNRSR-IVSPEKTNQCGGKGCPTLNETESC--LTNCCVVN--CQWTIWSVWSECSNSCG-DGESKRNRTFLKKAECGGEICVGETEELVNCSFFKNKDC-----IMDSWSEWSNCILANGKCGTGLVYRNRSIIKHE-------ECNGIPCSNIYESILCQGECCMQNCTISQWSEWSFSNVTC-------------GIGNQTRTRFILDKAECGGSSCPMDLIDIRQYEKSDYRNCSVSAWTNWSPCSNSCGSGYQVSSRYVVIDEACGGSCSMPLTRNQTCSSFADNLD---------CLVSPWSEWSECIKNCNNGIKYRTRNIIQLPKCKGISCPNLQDNSTCMES 410 The following BLAST results are available for this feature:
BLAST of Spondin-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Spondin-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Spondin-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Spondin-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Spondin-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30012850 ID=SMED30012850|Name=Spondin-1|organism=Schmidtea mediterranea sexual|type=transcript|length=2699bpback to top protein sequence of SMED30012850-orf-1 >SMED30012850-orf-1 ID=SMED30012850-orf-1|Name=SMED30012850-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=820bp MKIYILIWNILWMLLCLHWMIQESFAMNTRTNCFKMHKKTYQKVMMKDGEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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