SMED30012842

Overview
NameSMED30012842
Smed IDSMED30012842
Length (bp)1032
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30012842 (SMED30012842) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30012842 (SMED30012842) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012842

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30012842h1SMcG0020377 SmedASXL_010360SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
gutSMED30012842h1SMcG0004821 dd_Smed_v4_11532_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012842h1SMcG0004821 dd_Smed_v4_11532_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30012842h1SMcG0020377 dd_Smed_v4_11532_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012842h1SMcG0020377 dd_Smed_v4_11532_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30012842h1SMcG0004821 dd_Smed_v6_11532_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30012842h1SMcG0020377 dd_Smed_v6_11532_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30012842h1SMcG0020377 OX_Smed_1.0.16980ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30012842 vs. TrEMBL
Match: A0A419Q1H5 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_8277s PE=4 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 3.022e-42
Identity = 75/190 (39.47%), Postives = 115/190 (60.53%), Query Frame = -1 
Query:  130 ANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRN-GMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSI--PIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGH 690
            +     +  ++ WI IA  + I WW+  G+L+GSW+  +MIP+DSD+DI +   +   +  ++TDRK I   K +++TRPG HC  + G+RLDC+G+ V +L D CSFCGP AR+FH + + +DI+L +LE+  +      E  + D+ + R    P    FPL  C +MGL+VPCP D E+ +   YG 
Sbjct:  213 SRRESLYRTLQEWIRIANQSGILWWITYGTLLGSWRDGNMIPYDSDVDISILGSYEKRLRQLATDRKSIQKGKFNLVTRPGEHCVTSPGLRLDCDGHNVTSLQDTCSFCGPLARMFHQYGEYVDIYLVHLEVRVDVANNPIEFGYFDEGMDRDRVEPASVIFPLRTCKYMGLSVPCPNDPERFLTSVYGE 402
[Close]
BLAST of SMED30012842 vs. TrEMBL
Match: G7YSX8 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CLF_109802 PE=4 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 3.086e-42
Identity = 75/190 (39.47%), Postives = 115/190 (60.53%), Query Frame = -1 
Query:  130 ANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRN-GMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSI--PIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGH 690
            +     +  ++ WI IA  + I WW+  G+L+GSW+  +MIP+DSD+DI +   +   +  ++TDRK I   K +++TRPG HC  + G+RLDC+G+ V +L D CSFCGP AR+FH + + +DI+L +LE+  +      E  + D+ + R    P    FPL  C +MGL+VPCP D E+ +   YG 
Sbjct:  213 SRRESLYRTLQEWIRIANQSGILWWITYGTLLGSWRDGNMIPYDSDVDISILGSYEKRLRQLATDRKSIQKGKFNLVTRPGEHCVTSPGLRLDCDGHNVTSLQDTCSFCGPLARMFHQYGEYVDIYLVHLEVRVDVANNPIEFGYFDEGMDRDRVEPASVIFPLRTCKYMGLSVPCPNDPERFLTSVYGE 402
[Close]
BLAST of SMED30012842 vs. TrEMBL
Match: A0A4S2L6M5 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009663 PE=4 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 3.610e-42
Identity = 75/189 (39.68%), Postives = 114/189 (60.32%), Query Frame = -1 
Query:  133 ANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRN-GMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSI--PIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYG 690
            +     +  ++ WI +A  + I WW+  G+L+GSW+  +MIP+DSD+DI +   +   +  ++TDRK I   K +++TRPG HC  + G+RLDCNG  V +L D CSFCGP AR+FH + + +DI+  +LE+H +      E  + D+ + R    P    FPL  C +MGL+VPCP D E+ +   YG
Sbjct:  213 SRRESLYQTLQEWIKMANQSKILWWITYGTLLGSWRDGNMIPYDSDVDISILGSYEKRLRELATDRKAIQKGKFNLVTRPGEHCVTSPGLRLDCNGRNVTSLQDTCSFCGPLARMFHQYGEYVDIYPVHLEVHVDVANNPIEFGYLDEGMDRDRVDPASVIFPLRTCKYMGLSVPCPNDPERFLTSVYG 401
[Close]
BLAST of SMED30012842 vs. TrEMBL
Match: A0A075A604 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_12530 PE=4 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 9.649e-40
Identity = 74/190 (38.95%), Postives = 110/190 (57.89%), Query Frame = -1 
Query:  130 ANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRN-GMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSI--PIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGH 690
            +     +  ++ WI IA  + I WW+  G+L+GSW+  +MIP+DSD+DI +   +   +  ++TDRK I   K +++TRPG HC  + G+RLDC G  V +L D CSFCGP AR+F  + + IDI+  +LE+  +      E  + D+ + R    P    FPL  C +MGL VPCP D E+ +   YG 
Sbjct:  209 SRRESLYRTLQDWIRIANQSKILWWITYGTLLGSWRDGNMIPYDSDVDISILGSYEKRLRELATDRKSIQKGKFNLVTRPGEHCVTSPGLRLDCGGRNVTSLRDTCSFCGPLARMFRQYGEYIDIYPVHLEVRMDVANNPIEFGYLDEGMDRDRVEPASVIFPLRNCKYMGLNVPCPNDPERFLTSVYGK 398
[Close]
BLAST of SMED30012842 vs. TrEMBL
Match: A0A1S8WX06 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=X801_05152 PE=4 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 6.143e-39
Identity = 74/190 (38.95%), Postives = 110/190 (57.89%), Query Frame = -1 
Query:  130 ANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRN-GMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSI--PIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGH 690
            +     +  ++ WI IA  + I WW+  G+L+GSW+  +MIP+DSD+DI +   +   +  ++TDRK I   K +++TRPG HC  + G+RLDC G  V +L D CSFCGP AR+F  + + IDI+  +LE+  +      E  + D+ + R    P    FPL  C +MGL VPCP D E+ +   YG 
Sbjct:  209 SRRESLYRTLQDWIRIANQSKILWWITYGTLLGSWRDGNMIPYDSDVDISILGSYEKRLRELATDRKSIQKGKFNLVTRPGEHCVTSPGLRLDCGGRNVTSLRDTCSFCGPLARMFRQYGEYIDIYPVHLEVRMDVANNPIEFGYLDEGMDRDRVEPASVIFPLRNCKYMGLNVPCPNDPERFLTSVYGK 398
[Close]
BLAST of SMED30012842 vs. Ensembl Nematostella
Match: EDO45540 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSB7])

HSP 1 Score: 78.1814 bits (191), Expect = 9.891e-17
Identity = 65/206 (31.55%), Postives = 92/206 (44.66%), Query Frame = -1 
Query:   94 LMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKF---MNLIHNISTDRK--DISANKTSIITRPGPH---CPRNGMRLDCNGNPVHALVDGCSFCGPFAR-----VFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSIPIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGHFKYD--QLCINGTW 666
            L++ W  IA ++NIT+ L+ GSL+G  +  D++PWD D+DI V       +  I NI   R   DI+     ++  P         +  R DC G  V  LVD CS   P AR     VF D       F  Y E  DN + V   +       K  +     FPL +C  MG+   CPKD  +++   YG  +    + C +G W
Sbjct:   59 LLRTWNLIASHHNITYSLMYGSLLGVERTADILPWDHDLDIMVSYDGILKLERIANICDPRGHFDINDGLPHLVVLPNSEHNVFMDDRRRFDCAGKEVAKLVDACSIQEPLARLIAGSVFMD-------FYHYTEKEDNLISVPEGKTVT--YAKGEV-----FPLKECFLMGVKSWCPKDPIRVLETWYGKGRLGSHKSCQSGEW 250
[Close]
BLAST of SMED30012842 vs. Ensembl Nematostella
Match: EDO31192 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYN3])

HSP 1 Score: 78.1814 bits (191), Expect = 1.620e-16
Identity = 58/206 (28.16%), Postives = 98/206 (47.57%), Query Frame = -1 
Query:   85 DLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANK----TSIITRPGPH---CPRNGMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSIPIEWN-FPLHQCPFMGLTVPCPKDLEKIINFQYGHFKYDQ---LCINGTWKNN 669
            +L+  W     NNNI++ ++ GSL+G  +  D++ WD DID+ V  K +  +  ++        N      +++  P            RL C G  V  ++D CSF  P AR+       IDI+ +Y+E+        G+++F     KR +  + + FPL +C +MGL   CP++ E+++   YG    D     C +G W+N 
Sbjct:   90 ELLNYWNKFTKNNNISYAIMWGSLLGLLRTNDIMAWDYDIDVIVSLKDLVKLRKLAKAEGSFDYNDGNIHLAVMAEPEKSFNTSADKRARLSCRGEAVDKVMDPCSFVDPLARLLLKW-FFIDIY-TYVEV--------GDKLFALPEGKRFVMNKADMFPLQRCHYMGLECLCPRNPERLLEDWYGPGCVDANEFKCRDGEWQNQ 285
[Close]
BLAST of SMED30012842 vs. Ensembl Nematostella
Match: EDO45603 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSB6])

HSP 1 Score: 76.6406 bits (187), Expect = 6.751e-16
Identity = 60/223 (26.91%), Postives = 103/223 (46.19%), Query Frame = -1 
Query:   88 QSFIGDCSYPAN--HNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKD---ISANKTSIITRPGPHCPR---NGMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGE--EMFNDDLIKRSIPIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYG--HFKYDQLCINGTWKN 720
            +   GD + P N        +++ W +I   +NIT+ L+ GSLIG+ +  D+IPWD D+D+ VP K + ++  +   R      S  +T I   P     +   + +R DC+G    + VD CS   P  R++++    +D+F  Y +         G   EM  +D           +P+ +C F+G+   CP +   +++  YG    K  + C +G W N
Sbjct:  103 KEIYGDTNCPDNGRRKPLTAILQYWTEITAKHNITYALMWGSLIGAIRTNDVIPWDHDMDLVVPYKDILVLEKLGWPRSSDPYKSDGRTYINAIPSSEHNKSMDDRLRWDCSGKLTPSHVDLCSIQEPIVRLYNNG-AFLDVFHYYDQGETVVTIPEGHKLEMKRNDF----------YPIRECLFLGIKSKCPNNPASLLSKWYGLNFIKSPKTCKDGHWVN 314
[Close]
BLAST of SMED30012842 vs. Ensembl Nematostella
Match: EDO38600 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCH0])

HSP 1 Score: 75.485 bits (184), Expect = 3.623e-15
Identity = 73/261 (27.97%), Postives = 124/261 (47.51%), Query Frame = -1 
Query:   94 VVNKCEICQNRISVSVPEQGSLFTPQQCQIMNSNVSYQGYLDQSFIGDCSYPANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRK---DISANKTSIITR----PGPHCPRNGMRLDCNGNPV-HALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSIPIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYG-HFKYD-QLCINGTW 846
            +VN+ E+  +++S +V   GS        I  SN S   +        C    + N   DL+  W  +A  + I +++ SGSL+G ++ +D+IP+DSD+D+ V     + + +I++ R      + +KT ++ +      P   R  +R  C+G  V    VD CSF  P AR+    R  +DIF  +    +  +  +   M+ D   K+    E  FP+  C F+G    CP++ E ++   YG HF    ++C NG W
Sbjct:  223 MVNRVELPNSKLSGAVGASGS----TSRLIGTSNNSPPDW------PLCKDNPHRNTLRDLLASWHLLAAKHKIRYFITSGSLLGYYRNRDIIPYDSDVDVYVDVDSYSTLRSIASPRDFKFKTADSKTRLVVQMDYWGTPMDSR--IRTSCDGRTVDRGSVDPCSFQDPMARLIRRGRY-VDIFAYFTT--NGVVNTTPTPMYYDPTTKKYFKPEDFFPIKPCTFIGTPTYCPRNAETMLKTMYGEHFATPYKICKNGLW 468
[Close]
BLAST of SMED30012842 vs. Ensembl Nematostella
Match: EDO35576 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SL37])

HSP 1 Score: 54.6842 bits (130), Expect = 2.527e-8
Identity = 48/190 (25.26%), Postives = 82/190 (43.16%), Query Frame = -1 
Query:  130 LMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVP-SKFMNLIHNISTD-RKDI--SANKTSIITRPGPHCPRNGMRLDCNG------NPVHALVDGC-SFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSIPIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGH 666
            +++I+  IA +N + +WL SG+L+G+ + +  IPWD D DI +P   ++ L  N S +  +D+    +++    RPG       +R    G      NP       C  +C  +   +HD    ID+F++     D     SG                  FPL +  F G  +  P + + I+  +YG 
Sbjct:  130 MLRIFDQIARSNGLKYWLTSGTLLGAVRHRGFIPWDGDADIEMPLEDYIKLFTNHSQEFPRDVFFQNSESDPFLRPGDPAKERPLRHPVVGLYRRTWNPRLRDRKSCVGYCLEYGCKWHDG-IMIDVFIA----EDTLGDTSG-----------------VFPLKEMEFEGFPLYAPNNWKDILESKYGE 297
[Close]
BLAST of SMED30012842 vs. Planmine SMEST
Match: SMESG000043342.1 (SMESG000043342.1)

HSP 1 Score: 609.757 bits (1571), Expect = 0.000e+0
Identity = 295/300 (98.33%), Postives = 296/300 (98.67%), Query Frame = -1 
Query:   82 MTLLSQYFHKNAVTFFLIFCLVQINLICIFIYLNTYLSINQTNKSVVNKCEICQNRISVSVPEQGSLFTPQQCQIMNSNVSYQGYLDQSFIGDCSYPANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRNGMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSIPIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGHFKYDQLCINGTWKNNL 981
            MTLLSQYFHKNAVTFFLIFCLVQINLICIFIYLNTYLSINQTNKSVVNKCEI QN ISVS PEQG LFTPQQCQIMNSNVSYQGYLDQSFIGDCS+PANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRNGMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSIPIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGHFKYDQLCINGTWKNNL
Sbjct:    1 MTLLSQYFHKNAVTFFLIFCLVQINLICIFIYLNTYLSINQTNKSVVNKCEIFQNPISVSAPEQGGLFTPQQCQIMNSNVSYQGYLDQSFIGDCSFPANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRNGMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSIPIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGHFKYDQLCINGTWKNNL 300
[Close]
BLAST of SMED30012842 vs. Planmine SMEST
Match: SMESG000043342.1 (SMESG000043342.1)

HSP 1 Score: 469.544 bits (1207), Expect = 7.184e-169
Identity = 224/225 (99.56%), Postives = 225/225 (100.00%), Query Frame = -1 
Query:   82 MNSNVSYQGYLDQSFIGDCSYPANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRNGMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSIPIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGHFKYDQLCINGTWKNNL 756
            MNSNVSYQGYLDQSFIGDCS+PANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRNGMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSIPIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGHFKYDQLCINGTWKNNL
Sbjct:    1 MNSNVSYQGYLDQSFIGDCSFPANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRNGMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSIPIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGHFKYDQLCINGTWKNNL 225
[Close]
BLAST of SMED30012842 vs. Planmine SMEST
Match: SMESG000043345.1 (SMESG000043345.1)

HSP 1 Score: 262.307 bits (669), Expect = 7.316e-87
Identity = 124/240 (51.67%), Postives = 160/240 (66.67%), Query Frame = -1 
Query:   94 ISVSVPEQGSLFTPQQCQIMNSNVSYQGYLDQSFIGDCSYPANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRNGMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSIPIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGHFKYDQLCINGTW 813
            IS+    + S     + +IMN N+SYQGY+D S+  +C  P  H   F +MK WI+I   NNITWW+I GSLIGS + +DMIPWDSDIDIG+PS++ N++  IST R +IS +K +IITRPGPHCP NGMR++C G PV +  DGCSFCGPFAR+F     RIDI+L Y++IH+NQL++  E ++         P+ W FPL  C FMGL V CP   E I+N  YG FK    C+NG W
Sbjct:    9 ISLESIRKISQLESDKIKIMNENISYQGYMDSSYDRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYNGMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYGDFKMRSKCVNGKW 248
[Close]
BLAST of SMED30012842 vs. Planmine SMEST
Match: SMESG000043345.1 (SMESG000043345.1)

HSP 1 Score: 263.848 bits (673), Expect = 1.248e-86
Identity = 124/240 (51.67%), Postives = 160/240 (66.67%), Query Frame = -1 
Query:   94 ISVSVPEQGSLFTPQQCQIMNSNVSYQGYLDQSFIGDCSYPANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRNGMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSIPIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGHFKYDQLCINGTW 813
            IS+    + S     + +IMN N+SYQGY+D S+  +C  P  H   F +MK WI+I   NNITWW+I GSLIGS + +DMIPWDSDIDIG+PS++ N++  IST R +IS +K +IITRPGPHCP NGMR++C G PV +  DGCSFCGPFAR+F     RIDI+L Y++IH+NQL++  E ++         P+ W FPL  C FMGL V CP   E I+N  YG FK    C+NG W
Sbjct:   67 ISLESIRKISQLESDKIKIMNENISYQGYMDSSYDRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYNGMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYGDFKMRSKCVNGKW 306
[Close]
BLAST of SMED30012842 vs. Planmine SMEST
Match: SMESG000043355.1 (SMESG000043355.1)

HSP 1 Score: 248.825 bits (634), Expect = 1.186e-80
Identity = 129/299 (43.14%), Postives = 178/299 (59.53%), Query Frame = -1 
Query:   94 FHKNAVTF-FLIFCLVQINLICIFIYLNTYLSINQTNKSVVNKCEICQ---------NRISVSVPEQGSLFTPQQCQIMNSNVSYQGYLDQSFIGDCSYPANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRNGMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSIPIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGHFKYDQLCINGTW 960
            F +N   F F++ CL  +  + ++ Y    +SI  + K   +   +C+         N     V   G + TP Q Q+ NSN+SY+GY D  F  DC  P  H + F LMK WI    ++NI WWL  GSLIGS + KDM+ WDSDID+G+P+++ +L+ N+ST R +IS  + +IITRPGPHCP NG R+DC GN V + VDGC+FCGP ARVF++  KRIDI+L  ++IH+N+LK+  E  F         P+ W FPL  C FM L V CPK+LE ++   Y  F+    C  G+W
Sbjct:   14 FKENPKRFLFVLLCLFGLVFLSVYYYFFD-ISIFNSPKLEFSNSYLCKYFINNNETWNFTEFDVTTIGDM-TPIQLQLHNSNISYKGYSDLKFSKDCGIPGEHEQLFQLMKFWIKFTSDHNIPWWLAYGSLIGSIRHKDMVAWDSDIDLGMPTQYEHLLRNLSTSRLNISNKQVNIITRPGPHCPYNGPRMDCKGNLVTSQVDGCAFCGPLARVFYNSWKRIDIYLFDVQIHENKLKIVEEGTFEYMSEAPRSPLNWYFPLRNCSFMNLMVLCPKNLEAVLTDYYKTFQSLYKCSKGSW 310
The following BLAST results are available for this feature:
BLAST of SMED30012842 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012842 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012842 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012842 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012842 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012842 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012842 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012842 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A419Q1H53.022e-4239.47Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
G7YSX83.086e-4239.47Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
A0A4S2L6M53.610e-4239.68Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A075A6049.649e-4038.95Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A1S8WX066.143e-3938.95Uncharacterized protein OS=Opisthorchis viverrini ... [more]
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BLAST of SMED30012842 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012842 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012842 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012842 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO455409.891e-1731.55Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO311921.620e-1628.16Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO456036.751e-1626.91Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO386003.623e-1527.97Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO355762.527e-825.26Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30012842 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012842 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000043342.10.000e+098.33SMESG000043342.1[more]
SMESG000043342.17.184e-16999.56SMESG000043342.1[more]
SMESG000043345.17.316e-8751.67SMESG000043345.1[more]
SMESG000043345.11.248e-8651.67SMESG000043345.1[more]
SMESG000043355.11.186e-8043.14SMESG000043355.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012842 ID=SMED30012842|Name=SMED30012842|organism=Schmidtea mediterranea sexual|type=transcript|length=1032bp
TTAGTATCTAAAATTGATTAAAAATAACATTGTATAATTCTACGATTATT
ATCAGAAATAATTTCAAATTCAGAATTTTTACAAATTGTTTTTCCAAGTT
CCATTTATACAAAGTTGATCATATTTAAAATGTCCATATTGGAAATTTAT
TATTTTTTCAAGGTCTTTTGGACAGGGGACCGTTAATCCCATGAATGGAC
ATTGATGAAGCGGAAAATTCCATTCAATGGGAATTGACCGTTTGATGAGG
TCATCATTGAACATTTCTTCACCGCTAACTTTTAGTTGGTTGTCGTGAAT
TTCTAAATATGACAGGAAGATATCAATCCGTTTACGATGGTCATGAAATA
CTCTTGCAAATGGGCCACAGAATGAACAACCGTCAACCAAGGCATGGACT
GGATTACCGTTACAGTCTAATCTCATTCCGTTACGTGGGCAATGTGGACC
TGGTCTGGTAATAATACTTGTTTTATTGGCTGATATATCTTTTCGATCTG
TGCTGATATTGTGAATCAAATTCATGAACTTGGAGGGCACTCCAATATCA
ATATCACTGTCCCATGGTATCATATCTTTAAACTTCCAGGAACCAATTAA
AGATCCAGAAATAAGCCACCAAGTGATATTATTATTCCCAGCAATATCTA
TCCATATTTTCATCAAGTCGAAATACTCATTATGATTCGCCGGATAACTG
CAATCTCCGATGAAGCTCTGGTCCAAGTAGCCTTGATAAGAAACATTGGA
ATTCATAATTTGACATTGCTGCGGTGTAAATAAGCTTCCTTGTTCCGGAA
CTGAAACGGAAATTCGATTTTGACAAATCTCACATTTGTTTACCACTGAT
TTATTTGTTTGGTTTATTGATAAATATGTGTTTAGGTAAATAAAAATACA
AATTAAATTAATTTGTACTAAACAAAATATCAAGAAAAATGTGACAGCAT
TTTTATGGAAATATTGAGAGAGAAGAGTCATAATAAGTAATTCATTTTCA
GTTGCTTCGCTATTTATACCGGATTAATCCGA
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protein sequence of SMED30012842-orf-1

>SMED30012842-orf-1 ID=SMED30012842-orf-1|Name=SMED30012842-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=301bp
MTLLSQYFHKNAVTFFLIFCLVQINLICIFIYLNTYLSINQTNKSVVNKC
EICQNRISVSVPEQGSLFTPQQCQIMNSNVSYQGYLDQSFIGDCSYPANH
NEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPS
KFMNLIHNISTDRKDISANKTSIITRPGPHCPRNGMRLDCNGNPVHALVD
GCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSI
PIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGHFKYDQLCINGTWKNNL
*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000418head
PLANA:0000419prepharyngeal region
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR007074LicD_fam
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007074LicD familyPFAMPF04991LicDcoord: 116..222
e-value: 6.1E-9
score: 36.3
NoneNo IPR availablePANTHERPTHR13627:SF31FUKUTIN-RELATED PROTEINcoord: 102..289
NoneNo IPR availablePANTHERPTHR13627FUKUTIN RELATED PROTEINcoord: 102..289
NoneNo IPR availableTMHMMTMhelixcoord: 15..37